keyword
MENU ▼
Read by QxMD icon Read
search

Immunoelectrophoresis

keyword
https://www.readbyqxmd.com/read/27904188/cutaneous-manifestations-of-multiple-myeloma
#1
Binodini Behera, Monali Pattnaik, Bharti Sahu, Prasenjeet Mohanty, Swapna Jena, Liza Mohapatra
Multiple myeloma (MM) is a proliferative disorder of plasma cells which produce abnormal immunoglobulin proteins. Skin involvement is rarely found in this disorder. They are either specific or nonspecific lesions. We report four such interesting patients who presented to us initially with common dermatoses such as leukocytoclastic vasculitis, pyoderma gangrenosum, and vesiculobullous disorders and were subsequently diagnosed to have MM. There were no skeletal involvements or renal function abnormality at the time of presentation...
November 2016: Indian Journal of Dermatology
https://www.readbyqxmd.com/read/27703021/aspergillus-antibody-detection-diagnostic-strategy-and-technical-considerations-from-the-soci%C3%A3-t%C3%A3-fran%C3%A3-aise-de-mycologie-m%C3%A3-dicale-french-society-for-medical-mycology-expert-committee
#2
F Persat, C Hennequin, J P Gangneux
Until now, there has been no consensus on the best method for the detection of anti-Aspergillus antibodies, a key diagnostic tool for chronic aspergilloses. To better appreciate the usage of and confidence in these techniques, the Société Française de Mycologie Médicale (French Society for Medical Mycology; SFMM) performed a two-step survey of French experts. First, we administered an initial survey to French labs performing Aspergillus serology to depict usage of the different techniques available for Aspergillus serology...
October 4, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27688770/a-high-throughput-size-exclusion-chromatography-method-to-determine-the-molecular-size-distribution-of-meningococcal-polysaccharide-vaccine
#3
Imran Khan, K M Taufiqur Rahman, S M Saad Us Siraj, Mahbubul Karim, Abdul Muktadir, Arpan Maheshwari, Md Azizul Kabir, Zebun Nahar, Mohammad Mainul Ahasan
Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd...
2016: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/27680225/a-case-of-intravenous-immunoglobulin-dependent-chronic-inflammatory-demyelinating-polyneuropathy-of-pure-motor-form-responsive-to-mycophenolate-mofetil-therapy
#4
Motomi Arai
This report concerns a case of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) with frequent treatment-dependent relapses. A 75-year-old male presented with a 2-month history of progressive weakness of the limbs with no sensory symptoms. Neurological examination revealed normal cranial nerves, MRC grade 4 power in the proximal and distal muscles of the limbs, and generalized areflexia. However, the sensory examination results, serum immunoelectrophoresis, anti-HIV antibody, and vitamins B1 and B12 levels were normal...
October 28, 2016: Rinshō Shinkeigaku, Clinical Neurology
https://www.readbyqxmd.com/read/27604415/two-cases-of-feline-paraneoplastic-alopecia-associated-with-a-neuroendocrine-pancreatic-neoplasia-and-a-hepatosplenic-plasma-cell-tumour
#5
Chiara Caporali, Francesco Albanese, Diana Binanti, Francesca Abramo
BACKGROUND: Feline paraneoplastic alopecia (FPA) is a rare condition listed among the cutaneous paraneoplastic syndromes, which occurs in association with pancreatic carcinoma, cholangiocarcinoma, hepatocellular carcinoma and metastatic intestinal carcinoma. OBJECTIVES: To describe the clinicopathological findings of paraneoplastic alopecia in two cats each with an uncommon tumour not previously reported in association with FPA. ANIMALS: Paraneoplastic alopecia was associated with neuroendocrine pancreatic neoplasia in a Persian cat and with a hepatosplenic plasma cell tumour in a domestic short hair cat...
December 2016: Veterinary Dermatology
https://www.readbyqxmd.com/read/27387591/serial-multiple-crossed-immunoelectrophoresis-at-a-microscale-a-stamp-sized-2d-immunoanalysis-of-protein-c3-activation-caused-by-nanoparticles
#6
Jean-Baptiste Coty, Fanny Varenne, Jean-Jacques Vachon, Christine Vauthier
Crossed immunoelectrophoresis (C-IE) is used to detect and quantify specific proteins. An application allowed the evaluation of complement system activation by nanomaterials. The work aimed to improve the C-IE toward a higher throughput and less tedious method. A new concept was implemented to prepare and run agarose gels. The first and the second dimension of electrophoresis were performed on a single gel plate, prepared before the beginning of the analysis. Several samples were migrated simultaneously on the same migration line...
September 2016: Electrophoresis
https://www.readbyqxmd.com/read/27314477/recombinant-production-of-native-human-%C3%AE-1-antitrypsin-protein-in-the-liver-hepg2-cells
#7
Hajar Jaberie, Fakhraddin Naghibalhossaini
OBJECTIVES: Alpha-1 antitrypsin (A1AT) deficiency is associated with emphysema and liver disease. Only plasma-derived A1AT protein is available for augmentation therapy. Recombinant A1AT (recA1AT) protein expressed in various types of available hosts are either non-glycosylated or aberrantly glycosylated resulting into reduced stability and biological activity. To overcome these limitations, we have used the human liver HepG2 cell line to produce recA1AT protein. RESULTS: HepG2 cells were transfected by A1AT cDNA and cell populations were generated that stably overexpressed A1AT protein...
October 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27283885/accuracy-of-enzyme-linked-immunosorbent-assays-for-quantification-of-antibodies-against-aleutian-mink-disease-virus
#8
A H Farid, P P Rupasinghe
There is a growing interest among mink ranchers to select their stock for tolerance to the Aleutian mink disease virus (AMDV). Enzyme-linked immunosorbent assays (ELISA) are used to identify mink which have low anti-AMDV antibody titres and are expected to tolerate the AMDV infection. The objective of this study was to calculate the accuracy of three ELISA systems which were performed on blood or serum of AMDV-inoculated American mink (Neovison vison) at five laboratories in Canada, USA, Finland, the Netherlands and Denmark...
September 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/26954940/fractionation-of-source-materials-leads-to-a-high-reproducibility-of-the-sq-house-dust-mite-slit-tablets
#9
Helene Henmar, Sofie Marie Toft Frisenette, Karin Grosch, Kim Nielsen, Gary Smith, Susanne Sønderkær, Jørgen Nedergaard Larsen
BACKGROUND: The production of house dust mite (HDM) allergen products for allergy immunotherapy has traditionally been based on purified mite bodies or whole-mite culture, which are quite different source materials with a limited possibility for adjusting the chemical composition. The SQ HDM SLIT-tablet is a fast-dissolving pharmaceutical formulation that has been developed for sublingual immunotherapy (SLIT) of HDM respiratory allergic disease. OBJECTIVE: The objective of the present study was to establish a process for the production of drug substances for the SQ HDM SLIT-tablet offering a high reproducibility and independent control of the major allergens...
2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/26688629/immunodiagnosis-of-echinococcus-granulosus-infection-in-dogs-by-counter-immunoelectrophoresis-ciep
#10
Konanur Javaregowda Ananda, Placid Eugine D'Souza
The present study was undertaken for the detection of Echinococcus granulosus specific antibodies in dogs by counter immunoelectrophoresis (CIEP) with two different antigens viz., somatic and excretory secretory (E/S) antigens of E. granulosus adult worm. Totally 100 field serum samples were examined for the detection of anti-E. granulosus antibodies using somatic and E/S antigens of E. granulosus. The CIEP can able detect nine and eight E. granulosus specific antibodies with E/S and somatic antigen of E. granulosus, respectively...
December 2015: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/26638898/bimodal-immunoglobulin-a-gammopathy-in-a-cat-with-feline-myeloma-related-disorders
#11
Masaya Igase, Takako Shimokawa Miyama, Satoshi Kambayashi, Yumiko Shimoyama, Hiroko Hiraoka, Yumi Hirata, Miki Iwata, Kenji Baba, Takuya Mizuno, Masaru Okuda
A 10-year-old female spayed mixed breed cat with a subcutaneous mass on the right hind limb was revealed with bimodal monoclonal gammopathy composed of IgA by immunoelectrophoresis and immunofixation. Approximately 1 month after referral, the cat died due to renal failure. Postmortem immunohistopathologic evaluation of the subcutaneous mass revealed neoplastic cell proliferation of plasma cells and giant myeloma cells. Neoplastic cells were also present in the liver and spleen. These results led to the diagnosis of a rare case of feline myeloma-related disorders with extramedullary plasmacytoma infiltrating in multiple locations...
May 3, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/26634133/polyneuropathy-organomegaly-endocrinopathy-m-protein-and-skin-changes-poems-syndrome-a-paraneoplastic-syndrome
#12
Sunil Kumar, Shruti Sharma
POEMS syndrome (Crow-Fukase syndrome) is a rare paraneoplastic disorder. It is characterized by peripheral neuropathy, elevated vascular endothelial growth factors (VEGFs), monoclonal gammopathy, sclerotic bone lesions and Castleman disease. Other important clinical features are organomegaly, edema, ascites, papilledema, endocrinopathy, skin changes and thrombocytosis. A high index of suspicion, a detailed clinical history and examination followed by appropriate laboratory investigations like VEGF level, radiological skeletal survey and bone marrow biopsy are required to diagnose POEMS syndrome...
March 2015: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/26631887/parathyroid-hormone-related-peptide-producing-multiple-myeloma-and-renal-impairment
#13
Masaru Kinomura, Noriaki Shimada, Mana Nishikawa, Kazuyoshi Omori, Tomoyasu Jo, Yasunori Ueda, Kenji Notohara, Riko Kitazawa, Sohei Kitazawa, Masaki Fukushima, Kenichiro Asano
A 68-year-old man was hospitalized and examined for renal impairment. A laboratory analysis showed hypercalcemia. Although the serum parathyroid hormone and serum 1-25(OH)2 vitamin D3 levels were not elevated, the serum parathyroid hormone-related peptide (PTHrP) level was increased. Immunoelectrophoresis of the urine and bone marrow aspiration indicated multiple myeloma (MM). He was diagnosed with the coexistence of cast nephropathy and light chain deposition disease by a renal biopsy. Notably, PTHrP expression was detected in the myeloma cells based on immunohistochemistry and in situ hybridization...
2015: Internal Medicine
https://www.readbyqxmd.com/read/26622847/multiple-myeloma-with-intracranial-extension-and-bilateral-renal-infiltration-a-case-report-and-review-of-the-literature
#14
Min-Juan Zhang, Guo-Hong Su, Jie Shen, Feng-Hai Liu, Yan-Feng Xu, Xiao-Ling Zhang, Qing Wang, Rui-Huan Wang, Chun-Yan Liu, Yu-Lei Zhao
Multiple myeloma (MM) is a rare hematological malignancy, characterized by uncontrolled proliferation of plasma cells in the bone marrow. MM is usually confined to the bone marrow, however, it may occasionally infiltrate other tissues, which is known as extramedullary plasmacytoma (EMP). The majority of EMPs involve the head and neck region, although different anatomical sites, including the gastrointestinal tract, central nervous system, thyroid gland and breast may also be affected. The simultaneous presentation of EMP in the kidney and head is rare, presenting diagnostic challenges due to its unusual location and non-specific or absent symptoms...
October 2015: Oncology Letters
https://www.readbyqxmd.com/read/26587475/rocket-and-two-dimensional-immunoelectrophoresis-in-diagnosis-of-caprine-brucellosis
#15
Davood Mehrabani, Zahra Gholami, Jamshid Kohanteb, Masood Sepehrimanesh, Seyed Mohammad Hossein Hosseini
BACKGROUND: Brucellosis is a major bacterial zoonosis of global importance with the causative organisms of Gram-negative facultative intracellular pathogens. The aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. METHODS: Sera from 15 sheep, without any history of brucellosis vaccination, infected with Brucella melitensis M16 subcutaneously, were employed in a comparison of culture, precipitating, and immunoelectrophoretic tests...
August 2015: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/26582828/development-of-an-enzyme-linked-immunosorbent-assay-based-on-fusion-vp2332-452-antigen-for-detecting-antibodies-against-aleutian-mink-disease-virus
#16
Xiaowei Chen, Cailing Song, Yun Liu, Liandong Qu, Dafei Liu, Yun Zhang, Ming Liu
For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452 ELISA may be a preferable method for detecting antibodies against AMDV...
February 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/26568005/light-chain-deposition-disease-successfully-treated-with-bortezomib-in-an-elderly-patient-a-case-report-and-review-of-the-literature
#17
REVIEW
Yukihiro Wada, Masayuki Iyoda, Tomohiro Saito, Noriko Arai-Nunota, Ken Iseri, Eiko Tomita, Misa Ikeda, Yutaka Yamaguchi, Takanori Shibata
We herein report the case of an elderly patient with light-chain deposition disease (LCDD) successfully treated with bortezomib. An 83-year-old woman was admitted because of nephrotic syndrome. She was diagnosed to have monoclonal gammopathy of undetermined significance (IgG-κ type) and LCDD, on the basis of serum and urinary immunoelectrophoresis and renal biopsy. She responded to a modified regimen of bortezomib-based chemotherapy with disappearance of proteinuria without any adverse effects. According to a literature review of 16 cases, including the present case, bortezomib-based chemotherapy appears to be a convincing strategy for the treatment of LCDD even in elderly patients...
2015: Internal Medicine
https://www.readbyqxmd.com/read/26561877/identification-and-characterization-of-phytohemagglutinins-from-white-kidney-beans-phaseolus-vulgaris-l-var-beldia-in-the-rat-small-intestine
#18
Nader Nciri, Namjun Cho, Faiçal El Mhamdi, Abderraouf Ben Mansour, Fayçal Haj Sassi, Fatma Ben Aissa-Fennira
Although kidney bean (Phaseolus vulgaris L.) lectin toxicity is widely known, its effects in the gastrointestinal tract require further study. This investigation aimed to identify and characterize phytohemagglutinins (PHAs) in the small intestine and sera of rats following oral challenge with ground white beans. Twenty young, adult male rats were divided randomly into two groups of 10 animals each. The control group underwent gavage with a suspension of 300 mg of rodent pellet flour. The experimental group was administered a 300 mg Beldia bean flour suspension (BBFS)...
January 2016: Journal of Medicinal Food
https://www.readbyqxmd.com/read/26507447/possible-paraneoplastic-syndrome-case-of-bullous-pemphigoid-with-immunoglobulin-g-anti-bp180-c-terminal-domain-antibodies-associated-with-psoriasis-and-primary-macroglobulinemia
#19
Nobuki Maki, Toshio Demitsu, Naoka Umemoto, Kazutaka Nagashima, Toshinobu Nakamura, Maki Kakurai, Satoshi Nakamura, Tomoko Yamada, Norito Ishii, Takashi Hashimoto
A 61-year-old Japanese man developed bullous skin lesions during topical therapy for psoriasis vulgaris. Physical examination demonstrated numerous tense bullae and scaly erythemas on the trunk and extremities. Histopathology of the skin biopsy demonstrated subepidermal bullae and lymphocytic infiltration with eosinophils in the dermis. Direct immunofluorescence revealed linear deposits of immunoglobulin (Ig)G, IgA and C3 along the basement membrane zone. Indirect immunofluorescence of 1 mol/L NaCl-split skin showed IgG reactivity with both epidermal and the dermal sides...
May 2016: Journal of Dermatology
https://www.readbyqxmd.com/read/26484033/fibrillary-glomerulonephritis-combined-with-chronic-inflammatory-demyelinating-polyneuropathy
#20
Woo Kyung Sung, Jin Uk Jeong, Ki Tae Bang, Jong Ho Shin, Ji Hyung Yoo, Nak Min Kim, Jun Hyung Park, Joo Heon Kim
A 58-yr-old man presented with leg edema and subacute weakness of his bilateral lower extremities. Urinary and serum immunoelectrophoresis revealed the presence of lambda-type Bence Jones proteins. He was ultimately diagnosed with monoclonal gammopathy of undetermined significance (MGUS). A renal biopsy specimen showed fibrillary glomerulonephritis (FGN), which was randomly arranged as 12-20 m nonbranching fibrils in the basement membranes. Immunofluorescence studies were negative for immunoglobulin (Ig)G, IgM, IgA, C3, and kappa light chains in the capillary walls and mesangial areas...
June 2015: Kidney Research and Clinical Practice
keyword
keyword
17023
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"