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Immunoelectrophoresis

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https://www.readbyqxmd.com/read/28723285/case-244-systemic-amyloidosis-a-complication-of-waldenstr%C3%A3-m-macroglobulinemia
#1
Maja Hrabak-Paar, Marko Kralik
History A 68-year-old man was admitted to the hospital for work-up because of generalized fatigue, anorexia, chronic diarrhea, and weight loss. Laboratory work-up revealed an erythrocyte sedimentation rate of 58 mm/h (reference range, 3-23 mm/h), a hemoglobin level of 14.1 g/dL (reference range, 13.8-17.5 g/dL), a leukocyte count of 8.1 × 10(9)/L (reference range, [3.4-9.7] × 10(9)/L), a platelet count of 223 × 10(9)/L (reference range, [158-424] × 10(9)/L), an alkaline phosphatase level of 85 U/L (1.42 μkat/L) (normal level, <142 U/L [2...
August 2017: Radiology
https://www.readbyqxmd.com/read/28716013/long-term-renal-survival-of-%C3%AE-3-heavy-chain-deposition-disease-a-case-report
#2
Takayuki Katsuno, Shige Mizuno, Masatsuna Mabuchi, Naotake Tsuboi, Atsushi Komatsuda, Shoichi Maruyama
BACKGROUND: Monoclonal immunoglobulin deposition disease (MIDD) is characterized by the non-amyloid deposition of monoclonal immunoglobulin fragments in the basement membranes. Heavy chain deposition disease (HCDD) is a type of MIDD. HCDD is an extremely rare disease, and only three cases have been reported in Japan up to the present. The prognosis of HCDD is very poor, and optimal treatment has not been established. Only a few cases of HCDD with favorable long-term renal prognosis have been reported to date...
July 17, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28637843/poems-syndrome-a-rare-cause-of-exudative-ascites-and-chronic-peripheral-neuropathy
#3
Omar Al-Mayoof, Hana Al Sughaiyer, Wuroud Abuomar, Maria Khan
POEMS syndrome (Peripheral neuropathy, Organomegaly, Endocrinopathy, M protein, Skin changes) is a rare plasma cell disorder with multisystem involvementA 40-year-old man with a chronic history of unexplained peripheral neuropathy , presented with exudative ascites.He was found to have an incidental osteolytic lesion of the sacrum proven to be a plasmacytoma. Immunoelectrophoresis for monoclonal protein was negative.In addition, the patient was found to have hepatosplenomegaly, erectile dysfunction, hyperprolactinaemia and hypothyroidism...
June 20, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28620259/standardization-of-dot-enzyme-linked-immmunosorbent-assay-for-the-diagnosis-of-bovine-visceral-schistosomiasis
#4
Kommu Sudhakar, G S Sreenivasa Murthy, Gaddam Rajeshwari
AIM: Bovine visceral schistosomiasis has been reported as an important disease entity as it affects animal health, productivity, causes economic losses due to liver condemnation, and produces a high morbidity. This study was conducted to standardize an easy, reliable dot-enzyme-linked immmunosorbent assay (ELISA) for the diagnosis of visceral schistosomiasis caused by Schistosoma spindale and to know the prevalence rate in and around Hyderabad. MATERIALS AND METHODS: A dot-ELISA was standardized in the laboratory using whole worm antigen (WWA) and excretory-secretory antigen (ESA) of S...
May 2017: Veterinary World
https://www.readbyqxmd.com/read/28541149/characterization-of-equine-vitamin-d-binding-protein-development-of-an-assay-and-assessment-of-plasma-concentrations-of-the-protein-in-healthy-horses-and-horses-with-gastrointestinal-disease
#5
Tina H Pihl, Stine Jacobsen, Dorthe T Olsen, Peter Højrup, Astrid Grosche, David E Freeman, Pia H Andersen, Gunnar Houen
OBJECTIVE To purify and characterize equine vitamin D-binding protein (VDBP) from equine serum and to evaluate plasma concentrations of VDBP in healthy horses and horses with gastrointestinal injury or disease. ANIMALS 13 healthy laboratory animals (8 mice and 5 rabbits), 61 healthy horses, 12 horses with experimentally induced intestinal ischemia and reperfusion (IR), and 59 horses with acute gastrointestinal diseases. PROCEDURES VDBP was purified from serum of 2 healthy horses, and recombinant equine VDBP was obtained through a commercial service...
June 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28496948/evaluation-of-igy-antibody-as-a-polyspecific-coombs-reagent
#6
Esteban Justo Gutiérrez Calzado, Marlene Toledano Heredia, Jeorge Fernadez Duharte, Amir-Hassan Zarnani
BACKGROUND: During the last twenty years, the extraction of specific egg yolk (IgY) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. The aim of the present study was immunizing the chickens with Human Umbilical Cord Serum (HUCS) and the extraction of specific anti-human globulins (IgG, C3b, and C3d) antibodies from egg yolk in order to obtain polyspecific Coombs reagent. METHODS: The novelty of this work was the achievement of a polyclonal reagent through a very cheap alternative method in accordance with all ethical regulations required for obtaining it...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28480388/isolation-characterization-and-immunochemical-studies-on-fibrous-proteins-from-cowry-shell-cypraea-moneta-linnaeus
#7
R A Togun, R O Balogun, D O Adeyemi, T A Esan, G M Oyatogun, E O Oziegbe, R E Okonji, A Kuku
BACKGROUND: Biomaterials are non-drug substances used to treat, enhance or replace functions of body tissues or organs. Natural sources of biomaterials have recently become the focus of several research activities. Cowry shell constitutes one of the most promising natural sources of biomaterials because of its chemical stability, biodegradability and biocompatibility in the body. However, its applications may be limited due to immunogenic and toxic responses that may occur following implantation, hence this study...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28467313/igg-kappa-monoclonal-gammopathy-of-unknown-significance-with-al-amyloidosis-simulating-giant-cell-arteritis
#8
V M Pompilian, S Tanaseanu, Camelia Badea, Sabina Zurac, C Socoliuc, Sorina Badelita, Elena Botez, Mihaela Antohe
Monoclonal gammopathies complicated by AL amyloidosis can mimic giant cell arteritis (GCA).We hereby present the case of a 63 year old woman in whom symptoms consistent with GCA were the first manifestations of a monoclonal gammopathy of unknown significance (MGUS) associated with amyloidosis. A 63 year old woman was admitted for temporal headache, maseterine claudication, neck and shoulder stiffness. She was recently diagnosed with carpal tunnel syndrome. On physical examination she had prominent temporal arteries, macroglosia and orthostatic hypotension...
May 3, 2017: Romanian Journal of Internal Medicine, Revue Roumaine de Médecine Interne
https://www.readbyqxmd.com/read/28380026/the-role-of-subcutaneous-adipose-tissue-in-supporting-the-copper-balance-in-rats-with-a-chronic-deficiency-in-holo-ceruloplasmin
#9
Ekaterina Y Ilyechova, Nadezhda V Tsymbalenko, Ludmila V Puchkova
We have previously shown that (1) an acute deficiency in blood serum holo-ceruloplasmin (Cp) developed in rats that were fed fodder containing silver ions (Ag-fodder) for one month and (2) the deficiency in holo-Cp was compensated by non-hepatic holo-Cp synthesis in rats that were chronically fed Ag-fodder for 6 months (Ag-rats). The purpose of the present study is to identify the organ(s) that compensate for the hepatic holo-Cp deficiency in the circulation. This study was performed on rats that were fed Ag-fodder (40 mg Ag·kg-1 body mass daily) for 6 months...
2017: PloS One
https://www.readbyqxmd.com/read/28367947/a-case-of-amyloid-myopathy-diagnosed-during-the-treatment-of-myopathy-associated-with-anti-signal-recognition-particle-antibodies
#10
Nobuko Kawakami, Yusuke Katsuyama, Yuka Hagiwara, Hidefumi Yoshida, Kang Kim, Kiyoshi Harada
A 78-year-old man presented with subacute progressive proximal weakness and dysphagia. A biopsy specimen from the left biceps femoris revealed evidence of necrotic and regenerating muscle fibers, but lymphocyte infiltration was not noted. The patient was diagnosed with necrotizing myopathy with anti-signal recognition particle (SRP) antibodies. Concomitant therapy with prednisolone and azathioprine caused the serum CK level to return to normal and it caused clinical manifestations to abate. One year later, however, muscle weakness worsened...
April 28, 2017: Rinshō Shinkeigaku, Clinical Neurology
https://www.readbyqxmd.com/read/28249174/reduced-severity-of-histopathological-lesions-in-mink-selected-for-tolerance-to-aleutian-mink-disease-virus-infection
#11
A Hossain Farid, Linda E Ferns
The objective of this study was to measure the effect of selection for tolerance on the severity of the Aleutian disease (AD) lesions in mink. Sensitivity and specificity of antibody detection in the blood by counter-immunoelectrophoresis (CIEP) relative to the presence of Aleutian mink disease virus (AMDV) in the spleen by PCR in naturally infected farmed mink were also estimated. Carcasses of 680 sero-positive (CIEP-P) black mink from 28 farms in Nova Scotia, Canada, and from 132 sero-negative (CIEP-N) mink from 14 of these farms were collected at pelting time...
April 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/27904188/cutaneous-manifestations-of-multiple-myeloma
#12
Binodini Behera, Monali Pattnaik, Bharti Sahu, Prasenjeet Mohanty, Swapna Jena, Liza Mohapatra
Multiple myeloma (MM) is a proliferative disorder of plasma cells which produce abnormal immunoglobulin proteins. Skin involvement is rarely found in this disorder. They are either specific or nonspecific lesions. We report four such interesting patients who presented to us initially with common dermatoses such as leukocytoclastic vasculitis, pyoderma gangrenosum, and vesiculobullous disorders and were subsequently diagnosed to have MM. There were no skeletal involvements or renal function abnormality at the time of presentation...
November 2016: Indian Journal of Dermatology
https://www.readbyqxmd.com/read/27703021/aspergillus-antibody-detection-diagnostic-strategy-and-technical-considerations-from-the-soci%C3%A3-t%C3%A3-fran%C3%A3-aise-de-mycologie-m%C3%A3-dicale-french-society-for-medical-mycology-expert-committee
#13
F Persat, C Hennequin, J P Gangneux
Until now, there has been no consensus on the best method for the detection of anti-Aspergillus antibodies, a key diagnostic tool for chronic aspergilloses. To better appreciate the usage of and confidence in these techniques, the Société Française de Mycologie Médicale (French Society for Medical Mycology; SFMM) performed a two-step survey of French experts. First, we administered an initial survey to French labs performing Aspergillus serology to depict usage of the different techniques available for Aspergillus serology...
April 1, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27688770/a-high-throughput-size-exclusion-chromatography-method-to-determine-the-molecular-size-distribution-of-meningococcal-polysaccharide-vaccine
#14
Imran Khan, K M Taufiqur Rahman, S M Saad Us Siraj, Mahbubul Karim, Abdul Muktadir, Arpan Maheshwari, Md Azizul Kabir, Zebun Nahar, Mohammad Mainul Ahasan
Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd...
2016: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/27680225/a-case-of-intravenous-immunoglobulin-dependent-chronic-inflammatory-demyelinating-polyneuropathy-of-pure-motor-form-responsive-to-mycophenolate-mofetil-therapy
#15
Motomi Arai
This report concerns a case of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) with frequent treatment-dependent relapses. A 75-year-old male presented with a 2-month history of progressive weakness of the limbs with no sensory symptoms. Neurological examination revealed normal cranial nerves, MRC grade 4 power in the proximal and distal muscles of the limbs, and generalized areflexia. However, the sensory examination results, serum immunoelectrophoresis, anti-HIV antibody, and vitamins B1 and B12 levels were normal...
October 28, 2016: Rinshō Shinkeigaku, Clinical Neurology
https://www.readbyqxmd.com/read/27604415/two-cases-of-feline-paraneoplastic-alopecia-associated-with-a-neuroendocrine-pancreatic-neoplasia-and-a-hepatosplenic-plasma-cell-tumour
#16
Chiara Caporali, Francesco Albanese, Diana Binanti, Francesca Abramo
BACKGROUND: Feline paraneoplastic alopecia (FPA) is a rare condition listed among the cutaneous paraneoplastic syndromes, which occurs in association with pancreatic carcinoma, cholangiocarcinoma, hepatocellular carcinoma and metastatic intestinal carcinoma. OBJECTIVES: To describe the clinicopathological findings of paraneoplastic alopecia in two cats each with an uncommon tumour not previously reported in association with FPA. ANIMALS: Paraneoplastic alopecia was associated with neuroendocrine pancreatic neoplasia in a Persian cat and with a hepatosplenic plasma cell tumour in a domestic short hair cat...
December 2016: Veterinary Dermatology
https://www.readbyqxmd.com/read/27387591/serial-multiple-crossed-immunoelectrophoresis-at-a-microscale-a-stamp-sized-2d-immunoanalysis-of-protein-c3-activation-caused-by-nanoparticles
#17
Jean-Baptiste Coty, Fanny Varenne, Jean-Jacques Vachon, Christine Vauthier
Crossed immunoelectrophoresis (C-IE) is used to detect and quantify specific proteins. An application allowed the evaluation of complement system activation by nanomaterials. The work aimed to improve the C-IE toward a higher throughput and less tedious method. A new concept was implemented to prepare and run agarose gels. The first and the second dimension of electrophoresis were performed on a single gel plate, prepared before the beginning of the analysis. Several samples were migrated simultaneously on the same migration line...
September 2016: Electrophoresis
https://www.readbyqxmd.com/read/27314477/recombinant-production-of-native-human-%C3%AE-1-antitrypsin-protein-in-the-liver-hepg2-cells
#18
Hajar Jaberie, Fakhraddin Naghibalhossaini
OBJECTIVES: Alpha-1 antitrypsin (A1AT) deficiency is associated with emphysema and liver disease. Only plasma-derived A1AT protein is available for augmentation therapy. Recombinant A1AT (recA1AT) protein expressed in various types of available hosts are either non-glycosylated or aberrantly glycosylated resulting into reduced stability and biological activity. To overcome these limitations, we have used the human liver HepG2 cell line to produce recA1AT protein. RESULTS: HepG2 cells were transfected by A1AT cDNA and cell populations were generated that stably overexpressed A1AT protein...
October 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27283885/accuracy-of-enzyme-linked-immunosorbent-assays-for-quantification-of-antibodies-against-aleutian-mink-disease-virus
#19
A H Farid, P P Rupasinghe
There is a growing interest among mink ranchers to select their stock for tolerance to the Aleutian mink disease virus (AMDV). Enzyme-linked immunosorbent assays (ELISA) are used to identify mink which have low anti-AMDV antibody titres and are expected to tolerate the AMDV infection. The objective of this study was to calculate the accuracy of three ELISA systems which were performed on blood or serum of AMDV-inoculated American mink (Neovison vison) at five laboratories in Canada, USA, Finland, the Netherlands and Denmark...
September 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/26954940/fractionation-of-source-materials-leads-to-a-high-reproducibility-of-the-sq-house-dust-mite-slit-tablets
#20
Helene Henmar, Sofie Marie Toft Frisenette, Karin Grosch, Kim Nielsen, Gary Smith, Susanne Sønderkær, Jørgen Nedergaard Larsen
BACKGROUND: The production of house dust mite (HDM) allergen products for allergy immunotherapy has traditionally been based on purified mite bodies or whole-mite culture, which are quite different source materials with a limited possibility for adjusting the chemical composition. The SQ HDM SLIT-tablet is a fast-dissolving pharmaceutical formulation that has been developed for sublingual immunotherapy (SLIT) of HDM respiratory allergic disease. OBJECTIVE: The objective of the present study was to establish a process for the production of drug substances for the SQ HDM SLIT-tablet offering a high reproducibility and independent control of the major allergens...
2016: International Archives of Allergy and Immunology
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