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Nitika S Deshmukh, Vasudha A Belgaumkar
BACKGROUND: Acne has a prevalence of 90% among adolescents. Facial scarring affects 75% of patients. Autologous platelet-rich plasma (PRP) is a novel treatment option for acne scar management and can be used as an adjuvant to acne scar revision procedures. Owing to its remodeling properties, PRP when used as an adjuvant reduces the total number of sittings. OBJECTIVE: To compare efficacy of autologous PRP and subcision against subcision alone in acne scars. METHODOLOGY: It was an experimental analytical study conducted over a period of 2 years...
August 10, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
Matteo De March, Susana Barrera-Vilarmau, Emmanuele Crespan, Elisa Mentegari, Nekane Merino, Amaia Gonzalez-Magaña, Miguel Romano-Moreno, Giovanni Maga, Ramon Crehuet, Silvia Onesti, Francisco J Blanco, Alfredo De Biasio
p15PAF is an oncogenic intrinsically disordered protein that regulates DNA replication and lesion bypass by interacting with the human sliding clamp PCNA. In the absence of DNA, p15PAF traverses the PCNA ring via an extended PIP-box that contacts the sliding surface. Here, we probed the atomic-scale structure of p15PAF-PCNA-DNA ternary complexes. Crystallography and MD simulations show that, when p15PAF occupies two subunits of the PCNA homotrimer, DNA within the ring channel binds the unoccupied subunit. The structure of PCNA-bound p15PAF in the absence and presence of DNA is invariant, and solution NMR confirms that DNA does not displace p15PAF from the ring wall...
August 8, 2018: Nucleic Acids Research
Krister J Barkovich, Megan K Moore, Qi Hu, Kevan M Shokat
DEAD-box proteins are an essential class of enzymes involved in all stages of RNA metabolism. The study of DEAD-box proteins is challenging in a native setting since they are structurally similar, often essential and display dosage sensitivity. Pharmacological inhibition would be an ideal tool to probe the function of these enzymes. In this work, we describe a chemical genetic strategy for the specific inactivation of individual DEAD-box proteins with small molecule inhibitors using covalent complementarity...
August 8, 2018: Nucleic Acids Research
Jeffrey Kaye, Christina Reynolds, Molly Bowman, Nicole Sharma, Thomas Riley, Ona Golonka, Jonathan Lee, Charlie Quinn, Zachary Beattie, Johanna Austin, Adriana Seelye, Katherine Wild, Nora Mattek
An end-to-end suite of technologies has been established for the unobtrusive and continuous monitoring of health and activity changes occurring in the daily life of older adults over extended periods of time. The technology is aggregated into a system that incorporates the principles of being minimally obtrusive, while generating secure, privacy protected, continuous objective data in real-world (home-based) settings for months to years. The system includes passive infrared presence sensors placed throughout the home, door contact sensors installed on exterior doors, connected physiological monitoring devices (such as scales), medication boxes, and wearable actigraphs...
July 27, 2018: Journal of Visualized Experiments: JoVE
Vivekjot Brar, Gurpreet Kaur
OBJECTIVE: Esculin loaded nanoparticles for nasal delivery were prepared by ionic gelation technique using okra gum and chitosan Method: The preparation of nanoparticles was optimized using Box-Behnken experimental design employing particle size, entrapment efficiency and zeta potential as dependable variables. Okra concentration, chitosan concentration, pH and stirring speed were taken as independent variables. RESULTS: The formulations were found to depict particle size in the range of 294...
August 13, 2018: Pharmaceutical Nanotechnology
Anders Pape Møller, Javier Balbontín, André A Dhondt, Vladimir Remeš, Frank Adriaensen, Clotilde Biard, Jordi Camprodon, Mariusz Cichoń, Blandine Doligez, Anna Dubiec, Marcel Eens, Tapio Eeva, Anne E Goodenough, Andrew G Gosler, Lars Gustafsson, Philipp Heeb, Shelley A Hinsley, Staffan Jacob, Rimvydas Juškaitis, Toni Laaksonen, Bernard Leclercq, Bruno Massa, Tomasz D Mazgajski, Ruedi G Nager, Jan-Åke Nilsson, Sven G Nilsson, Ana C Norte, Rianne Pinxten, Hugo Robles, Tapio Solonen, Alberto Sorace, Arie J van Noordwijk, Marcel M Lambrechts
1.Co-existence between great tits Parus major and blue tits Cyanistes caeruleus, but also other hole nesting taxa, constitutes a classic example of species co-occurrence resulting in potential interference and exploitation competition for food and for breeding and roosting sites. However, the spatial and temporal variation in co-existence and its consequences for competition remain poorly understood. 2.We used an extensive database on reproduction in nest boxes by great and blue tits based on 87 study plots across Europe and Northern Africa during 1957-2012 for a total of 19,075 great tit and 16,729 blue tit clutches to assess correlative evidence for a relationship between laying date and clutch size, respectively, and density consistent with effects of intraspecific and interspecific competition...
August 12, 2018: Journal of Animal Ecology
Cheng Xiong, Dan Luo, Aihua Lin, Chunli Zhang, Libo Shan, Ping He, Bo Li, Qiaomei Zhang, Bin Hua, Zilv Yuan, Hanxia Li, Junhong Zhang, Changxian Yang, Yongen Lu, Zhibiao Ye, Taotao Wang
Carotenoids play important roles in many biological processes, such as light harvesting, photoprotection and visual attraction in plants. However, the regulation of carotenoid biosynthesis is still not fully understood. Here, we demonstrate that SlBBX20, a B-box (BBX) zinc-finger transcription factor, is a positive regulator of carotenoid accumulation in tomato (Solanum lycopersicum). Overexpression of SlBBX20 leads to dark green fruits and leaves and higher levels of carotenoids relative to the wild-type. Interactions between SlBBX20 and DE-ETIOLATED 1 (SlDET1) lead to the ubiquitination and 26S proteasome-mediated degradation of SlBBX20...
August 13, 2018: New Phytologist
Jon Ander Novella, Payam Emami Khoonsari, Stephanie Herman, Daniel Whitenack, Marco Capuccini, Joachim Burman, Kim Kultima, Ola Spjuth
Motivation: Computational biologists face many challenges related to data size, and they need to manage complicated analyses often including multiple stages and multiple tools, all of which must be deployed to modern infrastructures. To address these challenges and maintain reproducibility of results, researchers need (i) a reliable way to run processing stages in any computational environment, (ii) a well-defined way to orchestrate those processing stages, and (iii) a data management layer that tracks data as it moves through the processing pipeline...
August 8, 2018: Bioinformatics
Madhurendra Singh, Laura Bergmann, Alexander Lang, Katja Pexa, Fabian Kuck, Dennis Stibane, Linda Janke, Hakima Ezzahoini, Antje Lindecke, Constanze Wiek, Helmut Hanenberg, Karl Köhrer, Charlotte von Gall, Hans Reinke, Roland P Piekorz
The albumin D-box binding protein (DBP) is a member of the PAR bZip (proline and acidic amino acid-rich basic leucine zipper) transcription factor family and functions as important regulator of circadian core and output gene expression. Gene expression of DBP itself is under the control of E-box-dependent binding by the Bmal1-Clock heterodimer and CRE-dependent binding by the cAMP responsive element binding protein (CREB). However, the signaling mechanism mediating CREB-dependent regulation of DBP expression in the peripheral clock remains elusive...
July 13, 2018: Oncotarget
Xiaoting Fang, YongLiang Jiang, Kim Li, Qinglu Zeng
Background: There are six known families of homing endonucleases, LAGLIDADG, GIY-YIG, HNH, His-Cys box, PD-(D/E)-XK, and EDxHD, which are characterized by their conserved residues. Previously, we discovered a novel homing endonuclease F-CphI encoded by ORF177 of cyanophage S-PM2. F-CphI does not resemble any characterized homing endonucleases. Instead, the C-terminus of F-CphI aligns well with the N-terminal catalytic domain of a Holliday junction DNA resolvase, phage T4 endonuclease VII (Endo VII)...
2018: Mobile DNA
Robert Hotman Sirait, Mochammad Hatta, Muhammad Ramli, Andi Asadul Islam, Syafrie Kamsul Arief
Background: Severe musculoskeletal trauma can trigger an inflammatory response, and an excessive inflammatory response can lead to systemic inflammatory response syndrome and multiorgan failure. High-mobility group box 1 (HMGB1) is an early mediator pro-inflammatory cytokine in sterile injuries and a late cytokine mediator in infection and sepsis. Previous research has shown that administration of systemic lidocaine can inhibit HMGB1 expression in macrophages of septic rats. The aim of this study was to demonstrate the efficacy of systemic lidocaine to inhibit HMGB1 mRNA and protein in a BALB/c mouse model of sterile inflammation due to closed fracture musculoskeletal injury...
July 2018: Saudi Journal of Anaesthesia
Luca Campone, Rita Celano, Anna Lisa Piccinelli, Imma Pagano, Sonia Carabetta, Rosa Di Sanzo, Mariateresa Russo, Elena Ibañez, Alejandro Cifuentes, Luca Rastrelli
Food industry produces a large amount of onion wastes. Due to the high amount of bioactive compounds in onion by-products an idea for their reuse, could be use them as source of high-value functional and health ingredients. In this study, outer dry layers of coppery onion "Ramata di Montoro" were used as source of bioactive compounds. Firstly, the chemical profile of secondary metabolites of exhaustive extract, obtained by ultrasound assisted extraction was established by UHPLC-UV-HRMS/MS analysis...
December 15, 2018: Food Chemistry
Heloisa Gabriel Falcão, Cíntia Ladeira Handa, Mariah Benine Ramos Silva, Adriano Costa de Camargo, Fereidoon Shahidi, Louise Emy Kurozawa, Elza Iouko Ida
Ultrasound may convert conjugated isoflavones into their corresponding aglycones, the best form for absorption in the human body. However, ultrasound may also influence the activity of endogenous β-glucosidase. Therefore, the present work evaluated the effects of soybean ultrasound pre-treatment by applying the Box-Behnken design prior to soaking, a step that is important for industries to prepare certain soy products. Furthermore, a multi-response optimisation is provided. The best conditions for soybean ultrasound pre-treatment were established as temperature, X1  = 55 °C; exposure time, X2  = 5 min and ultrasound intensity, X3  = 19...
December 15, 2018: Food Chemistry
Ylenia Riciputi, Elixabet Diaz-de-Cerio, Hazal Akyol, Esra Capanoglu, Lorenzo Cerretani, Maria Fiorenza Caboni, Vito Verardo
Potato processing generates large amounts of by-products, which include potato peels and the outer layers of flesh, which contain phenolic compounds. The purpose of this study was to establish an extraction method for phenolic compounds from industrial potato by-products by using response surface methodology (RSM). Box-Behnken design (BBD) was performed to optimize the extraction conditions of phenolic compounds considering different extraction temperature, ratios of ethanol/water, time of extraction and sample/solvent ratio...
December 15, 2018: Food Chemistry
Michäel Duruisseaux, Anna Martínez-Cardús, Maria E Calleja-Cervantes, Sebastian Moran, Manuel Castro de Moura, Veronica Davalos, David Piñeyro, Montse Sanchez-Cespedes, Nicolas Girard, Marie Brevet, Etienne Giroux-Leprieur, Coraline Dumenil, Monica Pradotto, Paolo Bironzo, Enrica Capelletto, Silvia Novello, Alexis Cortot, Marie-Christine Copin, Niki Karachaliou, Maria Gonzalez-Cao, Sergio Peralta, Luis M Montuenga, Ignacio Gil-Bazo, Iosune Baraibar, Maria D Lozano, Mar Varela, Jose C Ruffinelli, Ramon Palmero, Ernest Nadal, Teresa Moran, Lidia Perez, Immaculada Ramos, Qingyang Xiao, Agustin F Fernandez, Mario F Fraga, Marta Gut, Ivo Gut, Cristina Teixidó, Noelia Vilariño, Aleix Prat, Noemi Reguart, Amparo Benito, Pilar Garrido, Isabel Barragan, Jean-François Emile, Rafael Rosell, Elisabeth Brambilla, Manel Esteller
BACKGROUND: Anti-programmed death-1 (PD-1) treatment for advanced non-small-cell lung cancer (NSCLC) has improved the survival of patients. However, a substantial percentage of patients do not respond to this treatment. We examined the use of DNA methylation profiles to determine the efficacy of anti-PD-1 treatment in patients recruited with current stage IV NSCLC. METHODS: In this multicentre study, we recruited adult patients from 15 hospitals in France, Spain, and Italy who had histologically proven stage IV NSCLC and had been exposed to PD-1 blockade during the course of the disease...
August 9, 2018: Lancet Respiratory Medicine
Masashi Minamino, Shoin Tei, Lumi Negishi, Masato T Kanemaki, Atsunori Yoshimura, Takashi Sutani, Masashige Bando, Katsuhiko Shirahige
Sister chromatid cohesion, mediated by cohesin, is required for accurate chromosome segregation [1, 2]. This process requires acetylation of cohesin subunit SMC3 by evolutionarily conserved cohesin acetyltransferases: Eco1 in budding yeast; XEco1 and XEco2 in Xenopus; and ESCO1 and ESCO2 in human [3-10]. Eco1 is recruited to chromatin through physical interaction with PCNA [11] and is degraded by the Skp1/Cul1/F-box protein complex after DNA replication to prevent ectopic cohesion formation [12]. In contrast, XEco2 recruitment to chromatin requires prereplication complex formation [13] and is degraded by the anaphase-promoting complex (APC) [14]...
August 1, 2018: Current Biology: CB
Fathollah Qafarizadeh, Minoo Kalantari, Noureddin Nakhostin Ansari, Alireza Akbarzadeh Baghban, Aliasqar Jamebozorgi
Upper extremity motor impairment is one of the most prevalent problems following stroke. Considering the functional importance of the upper extremity in the daily life, the purpose of this study was to investigate the effect of kinesiotaping (KT) on hand function and spasticity in individuals following a stroke. Eight individuals who had experienced a stroke, with their age ranging from 47 to 66, participated in this pretest-posttest clinical study. An I- strip of tape was placed on the extensor muscles of the forearm...
July 2018: Journal of Bodywork and Movement Therapies
Iman Vahdat, Farhad Tabatabai Ghomsheh
The main objective of this study was to investigate the effects of task execution variables on the value and point of application of the resultant vertical ground reaction force acting on the foot sole during squat lifting. This can be of particular importance since it may help to recognize the roles of the foot sole parts in bearing the support force during industrial and labor lifting activities. Twelve healthy men lifted a box with 4, 8 and 12 kg masses at fast and slow speeds. Ground reaction force and center of pressure were measured using two parallel Kistler force-plates...
July 2018: Journal of Bodywork and Movement Therapies
Hugo M Oliveira, Alice Q Pinheiro, António J M Fonseca, Ana R J Cabrita, Margarida R G Maia
The present report studied the role of ultrasound (US) energy in the amyloglucosidase-based starch hydrolysis using two complementary approaches: (i) in the activity of six commercially-available amyloglucosidases (using soluble starch as substrate), and (ii) in the hydrolysis of four pure starches from different botanical sources. This corresponds to the first systematic evaluation of the role of US in starch hydrolysis mediated by amyloglucosidase, being a consequence of our previous report that assessed the effect of US in the activity of alpha-amylase (LWT - Food Science and Technology 84 (2017) 674-685)...
July 29, 2018: Ultrasonics Sonochemistry
Yang Li, Tao Zhang, Yanhui Zhang, Xingkai Zhao, Wenbo Wang
Long non-coding RNAs (lncRNAs) play an important role in the proliferation and metastasis of osteosarcoma. Identification of the pathogenesis osteosarcoma and development of new therapeutic strategies against osteosarcoma are urgently needed. In this study, we evaluated the expression of TUG1 (Taurine Upregulated Gene 1) in osteosarcoma tissues and selected it as our target for further analyses. In vitro, we found that TUG1 was up-regulated by FOXM1 (Forkhead Box M1) in osteosarcoma cells. TUG1 accelerated osteosarcoma proliferation, migration, and invasion by competitively sponging miR-219a-5p, leading to up-regulation of PIK3CA and activation of the AKT signaling pathway...
August 12, 2018: Cancer Science
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