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Rui-Gang Zhang, Kewu Pan, Yuan Hao, Chung-Yin Yip, Wing-Hung Ko
Carbon monoxide (CO) is an anti-inflammatory gaseous molecule produced endogenously by heme oxygenases (HOs) HO-1 and HO-2. However, the mechanisms underlying the anti-inflammatory effects of CO in the human bronchial epithelium are still not fully understood. In this study, the cationic peptide poly-l-arginine (PLA) was utilized to induce bronchial epithelial damage and subsequent pro-inflammatory cytokine release in the human bronchial epithelial cell line 16HBE14o-. Expression of both HO-1 and HO-2 after PLA exposure was examined...
December 12, 2018: Molecular Immunology
Luckika Panthiya, Rungusa Pantan, Jiraporn Tocharus, Archawin Nakaew, Apichart Suksamrarn, Chainarong Tocharus
Tiliacorinine 12'-O-acetate is a modified analog of Tiliacorinine, a major compound in Tiliacora triandra. The present study explored the vasorelaxation property of tiliacorinine 12'-O-acetate and its mechanism in isolated rat aorta using the organ bath technique. Tiliacorinine 12'-O-acetate exhibited concentration-dependent (10-15 -10-3.5 M) vasorelaxation in endothelium-intact rings (Emax  = 93.53 ± 2.79%) and endothelium-denuded rings (Emax  = 74.31 ± 5.09%). The effects of tiliacorinine 12'-O-acetate were attenuated by pre-incubation with N(ω)-nitro-l-arginine methyl ester (L-NAME, endothelium nitric oxide synthase inhibitor) (100 μM), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, soluble quanylylcyclase inhibitor) (1 μM), and 4-aminopyridine (1 mM, Kv channel blocker)...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Akram Assali, Shayan Razzazan, Omid Akhavan, Fatemeh Mottaghitalab, Mohsen Adeli, Fatemeh Atyabi
Interaction of nanoplatforms with biomolecules in biological fluids alters nanoplatforms approach to target tissue and deliver their cargo. Here in, three nanoplatforms were utilized as a carrier to detect the effects of subsequent biomolecules on gene delivery using NIR thermal therapy. Nanoplatforms included; graphene oxide coated gold nanorods (NR@GO), PEGylated NR@GO (NR@GO-PEG) and poly L arginine functionalized NR@GO-PEG (NR@GO-PEG-PLArg). Results indicated that incubation of nanoplatforms in different concentrations of human plasma induced the evolution of layer of proteins (corona) with different thickness on the surface of nanoplatforms...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
Othman A Al-Shboul, Ayman G Mustafa, Amal Abu Omar, Ahmed N Al-Dwairi, Mohammad A Alqudah, Mona S Nazzal, Mahmoud A Alfaqih, Rami A Al-Hader
Previous studies have shown that progesterone could inhibit muscle contraction in various sites of the gastrointestinal tract. The underlying mechanisms responsible for these inhibitory effects of progesterone are not fully known. The aim of the current study was to investigate the effect of progesterone on the nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) pathway and muscle contraction in the stomach. Single gastric smooth muscle cells from female Sprague-Dawley rats were used. The expression of progesterone receptor (PR) mRNA was analyzed by reverse transcription polymerase chain reaction...
December 2018: Biomedical Reports
Chu Shan Tan, Yean Chun Loh, Yung Sing Ch'ng, Chiew Hoong Ng, Zhao Qin Yeap, Mariam Ahmad, Mohd Zaini Asmawi, Mun Fei Yam
ETHNOPHARMACOLOGICAL RELEVANCE: Citrus reticulatae Pericarpium (Chen pi) was widely used as an important ingredient in the prescription of TCM to treat phlegm fluid retention type hypertension. Since Chen pi is involved in treatment as antihypertensive TCM formula, we have reasonable expectation in believing that it might possess vasorelaxant activity. AIM OF THE STUDY: This study is designed to investigate the vasorelaxant effect of Chen pi and to study its pharmacology effects...
December 10, 2018: Journal of Ethnopharmacology
Sarah Ekeloef, Camilla Godthaab, Anne Marie V Schou-Pedersen, Jens Lykkesfeldt, Ismail Gögenur
BACKGROUND: Myocardial injury after noncardiac surgery is common and associated with major adverse cardiac events. Surgery induces acute endothelial dysfunction, which might be central in the pathophysiology of myocardial injury; however, the relationship between surgical stress and endothelial function remains incompletely understood. OBJECTIVES: This study aimed to assess the acute peri-operative changes in endothelial function after minor elective abdominal surgery...
December 12, 2018: European Journal of Anaesthesiology
Simon Lambden, James Tomlinson, Sophie Piper, Anthony C Gordon, James Leiper
BACKGROUND: Dimethylarginine dimethylaminohydrolase 2 (DDAH2) regulates the synthesis of nitric oxide (NO) through the metabolism of the endogenous inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA). Pilot studies have associated the rs805305 SNP of DDAH2 with ADMA concentrations in sepsis. This study explored the impact of the rs805305 polymorphism on DDAH activity and outcome in septic shock. METHODS: We undertook a secondary analysis of data and samples collected during the Vasopressin versus noradrenaline as initial therapy in septic shock (VANISH) trial...
December 11, 2018: Critical Care: the Official Journal of the Critical Care Forum
Alexander Bollenbach, Dimitrios Tsikas
Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are endogenous inhibitors of nitric oxide (NO) synthase. SDMA is excreted in the urine without major metabolization. About 10% of daily produced ADMA are excreted unchanged in the urine. The major elimination route of ADMA (about 90%) involves its hydrolysis to dimethylamine (DMA) and L-citrulline by dimethylarginine dimethylaminohydrolase (DDAH) and excretion of DMA in the urine. High circulating and low excretory concentrations of ADMA are considered risk factors...
December 8, 2018: Amino Acids
Hong-Hua Qiao, Lin-Nan Zhu, Yue Wang, Jia-Liang Hui, Wei-Bing Xie, Chao Liu, Ling Chen, Ping-Ming Qiu
Methamphetamine is an amphetamine-type psychostimulant that can damage dopaminergic neurons and cause characteristic pathological changes similar to neurodegenerative diseases such as Parkinson's disease. However, its specific mechanism of action is still unclear. In the present study, we established a Parkinson's disease pathology model by exposing SH-SY5Y cells and C57BL/6J mice to methamphetamine. In vitro experiments were performed with 0, 0.5, 1.0, 1.5, 2.0 or 2.5 mM methamphetamine for 24 hours or 2.0 mM methamphetamine for 0-, 2-, 4-, 8-, 16-, and 24-hour culture of SH-SY5Y cells...
February 2019: Neural Regeneration Research
Yi-Xuan Guo, Guo-Min Zhang, Xiao-Lei Yao, Ran Tong, Chun-Yu Cheng, Ting-Ting Zhang, Shu-Ting Wang, Hua Yang, Feng Wang
The aim of this study was to investigate effects of nitric oxide (NO) on steroidogenesis and apoptosis in goat luteinized granulosa cells (LGCs). We cultured goat LGCs from healthy follicles in culture medium supplemented with the NO donor sodium nitroprusside (SNP) or the NO synthase inhibitor Nω -Nitro-l-arginine methyl ester hydrochloride (l-NAME), then examined steroid synthesis, oxidative stress and apoptosis in vitro. The results showed that SNP treatment significantly increased the cGMP concentration in the LGCs (P < 0...
December 3, 2018: Theriogenology
Tatsuo Chiba, Kiyoshi Sakuma, Tetsuya Komatsu, Xin Cao, Megumi Aimoto, Yoshinobu Nagasawa, Kazuhiro Shimizu, Mao Takahashi, Yuichi Hori, Kohji Shirai, Akira Takahara
We assessed effects of acetylcholine and Nω-Nitro-l-arginine methyl ester hydrochloride (l-NAME) on the cardio-ankle vascular index (CAVI), an indicator of arterial stiffness from origin of aorta to tibial artery, in halothane-anesthetized rabbits. Acetylcholine decreased the blood pressure, femoral vascular resistance and CAVI, whereas l-NAME did not affect the CAVI at a hypertensive dose. The acetylcholine-induced decrement of CAVI was completely suppressed by l-NAME. These results suggest that the arterial stiffness in rabbits may be independent from homeostatic production of nitric oxide, however, it can be decreased by large amounts of nitric oxide that are intrinsically produced by exogenously administered acetylcholine...
November 24, 2018: Journal of Pharmacological Sciences
Ali Mahdi, John Pernow, Oskar Kovamees
BACKGROUND: Reduced bioavailability of nitric oxide (NO) is accompanied by endothelial dysfunction, which precedes clinical signs of atherosclerosis. The metalloenzyme arginase reciprocally inhibits the formation of NO and available data demonstrate that arginase contributes to reduced bioavailability of NO and increases the formation of reactive oxygen species. Emerging evidence suggest that arginase thereby plays a key role in the pathophysiology of age-associated vascular complications in animal models...
December 10, 2018: Rejuvenation Research
A Ran Jeon, Ji-Eun Kim
Redox modulation and S -nitrosylation of cysteine residues are the post-translational modifications of N -methyl-D-aspartate receptor (NMDAR) to regulate its functionality. Recently, we have reported that protein disulfide isomerase (PDI) reduces disulfide bond (S-S) to free thiol (-SH) on NMDAR. Since PDI is a modulator of S -nitrosylation on various proteins, it is noteworthy whether PDI affects S -nitrosylation of NMDAR in acute seizure and chronic epilepsy models. In the present study, we found that acute seizures in response to pilocarpine and spontaneous seizures in chronic epilepsy rats led to the reduction in S -nitrosylated thiol (SNO-thiol)-to-total thiol ratio on NMDAR, while they elevated nitric oxide (NO) level and S -nitrosylation on NMDAR...
2018: Frontiers in Cellular Neuroscience
Yolanda Corbett, Sarah D'Alessandro, Silvia Parapini, Diletta Scaccabarozzi, Parisa Kalantari, Stefania Zava, Flavio Giavarini, Donatella Caruso, Irma Colombo, Timothy J Egan, Nicoletta Basilico
BACKGROUND: Plasmodium falciparum haemozoin, a detoxification product of digested haemoglobin from infected erythrocytes, is released into the bloodstream upon schizont rupture and accumulates in leukocytes. High levels of haemozoin correlate with disease severity. Some studies have shown that concentrations of the substrate of inducible nitric oxide synthase (iNOS), L-arginine, as well as nitric oxide are low in patients infected with P. falciparum malaria. The present study investigates, in vitro, the role of P...
December 6, 2018: Malaria Journal
Consuelo Pasten, Cristóbal Alvarado, Jocelyn Rocco, Luis Contreras, Paula Aracena, Jéssica Liberona, Cristian Suazo, Luis Fernando Michea, Carlos E Irarrázabal
Upon renal ischemia-reperfusion (IR) injury, recruitment of neutrophils during the inflammatory process promotes local generation of oxygen and nitrogen reactive species which, in turn, are likely to exacerbate tissue damage. The mechanism by which iNOS is involved in IR has not been completely elucidated. In this work, the selective iNOS inhibitor L-N6 -(1-iminoethyl)lysine (L-NIL) and the NOS substrate L-Arginine (L-Arg), were employed to understand the role of NOS activity on the expression of particular target genes and the oxidative stress elicited after a 30 min of bilateral renal ischemia, followed by 48h reperfusion in BALB/c mice...
December 5, 2018: American Journal of Physiology. Renal Physiology
Merhan Mamdouh Ragy, Sabreen Mahmoud Ahmed
Diabetes and cardiometabolic risk factors including hypertension and dyslipidemia are the major threats to human health in the 21st century. Apoptosis in pancreatic tissue is one of the major causes of diabetes type 1 progression. The aim of this study was to investigate the effects of C-peptide or l-arginine on some cardiometabolic risk factors, pancreatic morphology, function and apoptosis, and the mechanisms of their actions. Forty adult male albino rats were divided into four equal groups: 1-control nondiabetic, 2-diabetic (no treatment), 3-diabetic + C-peptide, and 4-diabetic +  l-arginine...
December 4, 2018: Journal of Cellular Physiology
Kamil Kus, Agnieszka Kij, Agnieszka Zakrzewska, Agnieszka Jasztal, Marta Stojak, Maria Walczak, Stefan Chlopicki
BACKGROUND: The early detection of metastasis based on biomarkers in plasma may improve cancer prognosis and guide treatment. The aim of this work was to characterize alterations in metabolites of the arginine pathway, energy metabolism, and structural and signalling lipids in plasma in the early and late stages of murine breast cancer metastasis. METHODS: Mice were orthotopically inoculated with 4T1 metastatic breast cancer cells, and plasma was analysed along the pulmonary metastasis progression using LC-MS/MS-based targeted metabolomics and lipidomics...
December 4, 2018: Breast Cancer Research: BCR
Wen-Tsan Weng, Chieh-Shan Wu, Feng-Sheng Wang, Chang-Yi Wu, Yi-Ling Ma, Hoi-Hung Chan, Den-Chiung Wu, Jian-Ching Wu, Tian-Huei Chu, Shih-Chung Huang, Ming-Hong Tai
α-melanocyte-stimulating hormone (α-MSH) has been characterized as a novel angiogenesis inhibitor. The homeostasis of nitric oxide (NO) plays an important role in neovascularization. However, it remains unclear whether α-MSH mitigates angiogenesis through modulation of NO and its signaling pathway. The present study elucidated the function and mechanism of NO signaling in α-MSH-induced angiogenesis inhibition using cultured human umbilical vein endothelial cells (HUVECs), rat aorta rings, and transgenic zebrafish...
November 30, 2018: International Journal of Molecular Sciences
Xin Yu, Liang Ge, Liang Niu, Xin Lian, Haichun Ma, Lei Pang
Nitric oxide synthases (NOSs) are a family of enzymes that are responsible for the synthesis of nitric oxide (NO) from the amino acid L-arginine in the body. Among the three key NOSs, the expression of inducible NOS (iNOS) can only be induced by inflammatory stimuli and contribute to the large amount of NO production. iNOS-derived NO plays an important role in various physiological and pathophysiological conditions, including the ischemic heart disease. Nowadays, the development of specific iNOS inhibitors and the availability of iNOS knockout mice have provided substantial evidence to support the role of iNOS/NO signaling in the myocardium...
2018: Oxidative Medicine and Cellular Longevity
Chen Lu, Xu Huang, Hong-Li Lu, Shao-Hua Liu, Jing-Yu Zang, Yu-Jia Li, Jie Chen, Wen-Xie Xu
AIM: To investigate the distribution and function of interstitial cells of Cajal (ICCs) and platelet-derived growth factor receptor-α positive (PDGFRα+ ) cells in the proximal and distal colon. METHODS: The comparison of colonic transit in the proximal and distal ends was performed by colonic migrating motor complexes (CMMCs). The tension of the colonic smooth muscle was examined by smooth muscle spontaneous contractile experiments with both ends of the smooth muscle strip tied with a silk thread...
November 28, 2018: World Journal of Gastroenterology: WJG
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