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Hossein Tavallali, Gohar Deilamy-Rad, Narges Mosallanejad
In the current study, we reported a novel label-free and facile colorimetric approach for the sequential detection of copper ion (Cu2+ ), L-arginine (Arg), and L-cysteine (Cys) in the H2 O (10.0 mmol L-1 HEPES buffer solution, pH 7.0) using Reactive Blue 4 (RB4). First, the presence of Cu2+ led to a naked-eye color and spectral changes according to the binding site-signaling subunit approach. Then, the RB4-Cu2+ complex was successfully applied for Cys and Arg through different recognition pathways. The optical signals for Arg were observed due to its association involving the amino group, as well as the participation of the carboxylate group in a bidentate form to the complex, while selective behavior for Cys was explained by a metal displacement mechanism...
August 13, 2018: Applied Biochemistry and Biotechnology
Meiyu Huang, Honglian Ai, Xiaoxiang Xu, Ke Chen, Hong Niu, Huihui Zhu, Jie Sun, Dongyun Du, Liang Chen
Tall fescue (Festuca arundinacea Schreb) was widely studied for phytoremediation of organic or heavy metal contaminated soils. However, there is still little information concerning toxicity of chromium (Cr) to tall fescue and roles of nitric oxide (NO) in plants against Cr(VI) stress. In this study, different Cr(VI) treatments (0, 1, 5 and 10 mg/L Cr(VI)) and NO treatments were applied with different combinations in hydroponics culture and their interactions to tall fescue were studied. Specifically, 100 µM sodium nitroprusside (SNP) and 100 µM NG -nitro-L-arginine-methyl ester (L-NAME) treatments were used to apply exogenous NO or inhibit synthesis of NO respectively...
August 7, 2018: Ecotoxicology and Environmental Safety
X Y Zhang, M K Zhu, C Yuan, X T Zou
The goal of this study was to investigate the influence of l-arginine (l-Arg) supplementation on diet-induced changes in hypothalamus and liver proteome of laying hens. Layers were fed either an isonitrogenous control diet or a l-Arg diet. The test included a 2-week acclimation period and a 12-week experimental period. Eight layers per group were sacrificed at terminal of the experiment underwent 12 fasting. Blood and tissue samples of hypothalamus and liver were collected for further analysis. The levels of serum nitric oxide and hypothalamus neuropeptide Y of layers in l-Arg group were increased in comparison with those in control group...
August 8, 2018: Journal of Animal Physiology and Animal Nutrition
Jian Ying Yang, Yong Fa Zhang, Yuan Xiao Li, Xiang Ping Meng, Jian Feng Bao
To explore the protective mechanism of l-arginine against T-2 toxin-induced oxidative damage in mouse Leydig cells, Leydig cells were isolated and cultured with control, T-2 toxin (10 nM), l-arginine (0.25, 0.5, and 1.0 mM), and T-2 toxin (10 nM T-2 toxin) with l-arginine (0.25, 0.5, or 1.0 mM) for 24 hours. Cells and supernatants were harvested to examine cell viability, activities, and messenger RNA (mRNA) expression of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), malondialdehyde (MDA) content, and DNA damage...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Chao Wang, Cuicui Kang, Yihan Xian, Mingyu Zhang, Xiaolin Chen, Mingcai Pei, Weiyun Zhu, Suqin Hang
Nutrients regulate the secretion of gut satiety hormones, which is related to the modulation of food intake and blood glucose levels. Calcium-sensing receptor (CaSR) is involved in regulating gut hormone secretion in response to l-amino acids and multivalent cations. Rodents are often used to investigate the effect of nutrients on these hormonal release. However, results obtained using rodent models are difficult to be applied in humans, we used pigs as a model in this study because their physiology is similar to that of humans...
August 8, 2018: Journal of Food Science
Bernardo J Krause, Paola Casanello, Ana C Dias, Paulina Arias, Victoria Velarde, German A Arenas, Marcelo D Preite, Rodrigo Iturriaga
Chronic intermittent hypoxia (CIH), the main attribute of obstructive sleep apnea (OSA), produces oxidative stress, endothelial dysfunction, and hypertension. Nitric oxide (NO) plays a critical role in controlling the vasomotor tone. The NO level depends on the L-arginine level, which can be reduced by arginase enzymatic activity, and its reaction with the superoxide radical to produce peroxynitrite. Accordingly, we hypothesized whether a combination of an arginase inhibitor and an antioxidant may restore the endothelial function and reduced arterial blood pressure (BP) in CIH-induced hypertensive rats...
2018: Frontiers in Physiology
Arshia, Almas Jabeen, Aisha Faheem, Khalid Mohammed Khan, Shazia Shah, Shahnaz Perveen
BACKGROUND: Inflammation is a biological rejoinder of vascular tissues against destructive agents e.g. irritants, damaged cell or pathogens. During inflammation respiratory burst occur by activated phagocytes helps to destroy invading pathogens. Phagocytic cells such as neutrophils and macrophages are one of the major sources of reactive oxygen species (ROS) and nitric oxide (NO). Normally the redox environment is maintained by various antioxidant defense systems, however, these reactive oxygen species may be destructive and can lead to various pathological conditions...
August 5, 2018: Medicinal Chemistry
Andrea E Vasconez, Patrick Janetzko, James A Oo, Beatrice Pflüger-Müller, Corina Ratiu, Lunda Gu, Kristian Helin, Gerd Geisslinger, Ingrid Fleming, Katrin Schröder, Christian Fork, Ralf P Brandes, Matthias S Leisegang
AIM: The histone demethylase Jarid1b limits gene expression by removing the active methyl-mark from histone3-lysine4 at gene promoter regions. A vascular function of Jarid1b is unknown, but a vasoprotective function to inflammatory and hypertrophic stimuli, like Angiotensin II (AngII) could be inferred. This hypothesis was tested using Jarid1b knockout mice and the inhibitor PBIT. METHODS: Mice or aortic segments were treated with AngII to induce endothelial dysfunction...
August 4, 2018: Acta Physiologica
Xiaofeng Ou, Yusi Hua, Xuelian Liao, Cansheng Gong, Yan Kang
Cognitive impairments induced by severe acute pancreatitis (SAP) are severe complications, for which there are a lack of effective pharmacological treatment strategies. Berberine is an isoquinoline alkaloid extracted from the Chinese herb, Coptis rhizome, which exhibits numerous biological effects on gastrointestinal disorders. However, the effects of berberine on SAP‑induced cognitive impairments remain unknown. The present study aimed to investigate the effects of berberine on cognitive impairments associated with SAP...
September 2018: Molecular Medicine Reports
Xiao-Xiao Gao, Xiao-He Li, Qi-Fan Zhang, Ming Zhu, Yi-Xuan Guo, Kai-Ping Deng, Guo-Min Zhang, Feng Wang
Nutrient deficiency in ruminants can lead to estrus cycle disorders, a decreased pregnancy rate, and reduce birth weight. l-arginine (L-Arg), an important amino acid, can improve uterine homeostasis in pregnant sheep and prevent intrauterine growth restriction (IUGR). However, most studies of L-Arg have been conducted on pregnant sheep and few have reported the effects of L-Arg on microvessel density (MVD) in the non-pregnant ovine endometrium. The processes of normal uterine cyclical development and implantation are dependent on a balanced of endometrial MVD...
July 19, 2018: Theriogenology
Sanaa Ben Djemaa, Stephanie David, Katel Hervé-Aubert, Annarita Falanga, Stefania Galdiero, Emilie Allard-Vannier, Igor Chourpa, Emilie Munnier
The development of an efficient small interfering RNA (siRNA) delivery system has held scientists interest since the discovery of the RNA interference mechanism (RNAi). This strategy gives hope for the treatment of many severe diseases. Herein, we developed hybrid nanovectors able to deliver siRNA to triple negative breast cancer cells. The nanovectors are based on PEGylated superparamagnetic iron oxide nanoparticles (SPION) functionalized with gH625 peptide, chitosan and poly-L-arginine. Every component has a key role and specific function: SPION is the core scaffolding the nanovector; PEG participates in the colloidal stability and the immune stealthiness; gH625 peptide promotes the nanovector internalization into cancer cells; cationic polymers provide the siRNA protection and favor siRNA endosomal escape and delivery to cytosol...
July 28, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Joshua A Beckman, Shelley Hurwitz, Naomi D L Fisher
Neurovascular coupling, the relationship between cerebral blood flow and neuronal activity, is attenuated in patients with impaired executive function. We tested the hypothesis that peripheral vascular function may associate with executive function in older subjects with cardiovascular risk factors and that treatment with the antioxidant L-arginine would improve both vascular and executive function. Nineteen subjects with type 2 diabetes mellitus and/or controlled hypertension were enrolled. Subjects were treated with L-arginine or placebo for 4 days in a randomized, double-blinded, cross-over study...
July 10, 2018: Journal of the American Society of Hypertension: JASH
Moshe Shashar, Tamar Hod, Tamara Chernichovski, Avital Angel, Shaul Kazan, Ayelet Grupper, Sivan Naveh, Orit Kliuk-Ben Bassat, Talia Weinstein, Idit F Schwartz
Blockade of the mineralocorticoid receptor (MCR) has been shown to improve endothelial function far beyond blood pressure control. In the current studies we have looked at the effect of MCR antagonists on cationic amino acid transporter-1 (CAT-1), a major modulator of endothelial nitric oxide (NO) generation. Using radio-labeled arginine, {[3 H] l-arginine} uptake was determined in human umbilical vein endothelial cells (HUVEC) following incubation with either spironolactone or eplerenone with or without silencing of MCR...
July 26, 2018: Nitric Oxide: Biology and Chemistry
Aric F Logsdon, James S Meabon, Marcella M Cline, Kristin M Bullock, Murray A Raskind, Elaine R Peskind, William A Banks, David G Cook
Mild blast-induced traumatic brain injury (TBI) is associated with blood-brain barrier (BBB) disruption. However, the mechanisms whereby blast disrupts BBB integrity are not well understood. To address this issue BBB permeability to peripherally injected 14 C-sucrose and 99m Tc-albumin was quantified in ten brain regions at time points ranging from 0.25 to 72 hours. In mice, repetitive (2X) blast provoked BBB permeability to 14 C-sucrose that persisted in specific brain regions from 0.25 to 72 hours. However, 99m Tc-albumin revealed biphasic BBB disruption (open-closed-open) over the same interval, which was most pronounced in frontal cortex and hippocampus...
July 27, 2018: Scientific Reports
Deyna Francélia Andrade Próspero, Antonio Carlos Reis Filho, Celyane Alves Piauilino, Everton Moraes Lopes, Damião Pergentino de Sousa, Fernanda Regina de Castro Almeida
Previous studies have shown that isopulegol has anxiolytic, anticonvulsant, gastro-protective and antioxidant activities in rodents, but until now there are no studies showing activity of isopulegol in animal models of nociception and inflammation. The objective of this study was to evaluate the antinociceptive effect of isopulegol and to propose possible mechanisms involved in its effects observed in mice. Groups of male and female Swiss mice (20-35 g, n = 5-8) were used in this test under the authorization of Ethics Committee on Animal Experimentation (CEEA/UFPI N° 82/2014)...
July 24, 2018: Chemico-biological Interactions
Andriy L Zagayko, Tetiana Ye Kolisnyk, Olena I Chumak, Olena A Ruban, Oleh M Koshovyi
Background Vaccinium myrtillus leaves are known to be rich in phenols and have been used in traditional medicine as an antidiabetic remedy. This study evaluated the powder extract of V. myrtillus leaves obtained with the use of L-arginine and myo-inositol for anti-obesity and lipid-lowering potential in hamsters. Methods Standard phytochemical methods were used to determine the total phenolic and total flavonoid contents of the extract. The obesity condition was induced in Syrian hamsters by feeding them with highly palatable fat- and sugar-rich diet (40...
July 27, 2018: Journal of Basic and Clinical Physiology and Pharmacology
Andrea Berenyiova, Ima Dovinova, Miroslava Kvandova, Frantisek Kristek, Eugene Jansen, Miroslava Majzunova, Sona Cacanyiova
Although the role of nitric oxide (NO) in essential hypertension is still unclear, the effects of long-term NO deficiency have not yet been investigated during the critical juvenile period in spontaneously hypertensive rats (SHR). We aimed to analyze the effects of chronic NO synthase (NOS) inhibition on systolic blood pressure (sBP), vasoactivity, morphological changes and superoxide level in the thoracic aorta (TA), NOS activity in different tissues, and general biomarkers of oxidative stress in plasma of young SHR...
2018: Oxidative Medicine and Cellular Longevity
Isabel Prieto, Ana B Segarra, Ana B Villarejo, Marc de Gasparo, María M Martínez-Cañamero, Manuel Ramírez-Sánchez
BACKGROUND AND OBJECTIVE: Hypertension can lead to mood disorders that may worsen or ameliorate depending on the type of antihypertensive prescribed. Depression is associated with modifications in basal brain asymmetry particularly that of the frontal cortex, which is involved in blood pressure control. Furthermore, different vasoactive drugs may change the brain's asymmetry in a manner that contributes to cognition status. We studied the bilateral activity of several neuropeptidases in frontal cortex as a reflect of the functional status of certain neuropeptides involved in mood...
July 23, 2018: Journal of Hypertension
Seiji Fujigaki, Shin Nishiumi, Takashi Kobayashi, Makoto Suzuki, Takao Iemoto, Takashi Kojima, Yoshinori Ito, Hiroyuki Daiko, Ken Kato, Hirokazu Shouji, Kazufumi Honda, Takeshi Azuma, Masaru Yoshida
AIM: To identify the serum metabolomics signature that is correlated with the chemoradiosensitivity of esophageal squamous cell carcinoma (ESCC). MATERIALS & METHODS: Untargeted and targeted metabolomics analysis of serum samples from 26 ESCC patients, which were collected before the neoadjuvant chemoradiotherapy, was performed. RESULTS: On receiving the results of untargeted metabolomics analysis, we performed the targeted metabolomics analysis of the six metabolites (arabitol, betaine, glycine, L-serine, L-arginine and L-aspartate)...
August 2018: Biomarkers in Medicine
Vaibhav Walia, Chanchal Garg, Munish Garg
Present study was carried out to investigate the 'anxiolytic-like' effect of pyridoxine in mice. Pyridoxine (90, 180 and 360 mg/kg) was administered by intraperitoneal (i.p.) route to the experimental mice and anxiety-related behavior was evaluated by light and dark box (LDB) and elevated plus maze (EPM) models. Glutamate, GABA and nitrite levels were also determined in the isolated whole brain of mice. It was observed that pyridoxine (180 mg/kg, i.p.) exerted 'anxiolytic-like' effect in mice in EPM and LDB models...
July 21, 2018: Pharmacology, Biochemistry, and Behavior
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