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Hamed Nosrati, Marziyeh Salehiabar, Zahra Bagheri, Hamid Rashidzadeh, Soodabeh Davaran, Hossein Danafar
This study is a report about the synthesis iron oxide magnetic nanoparticles (IONPs) which modified with positive and negative charged amino acids (AAs). L-arginine (Arg) and L-aspartic acid (Asp) which have of guanidinium and carboxylic acid groups, respectively, were selected for this study. After loading Chrysin in amino acids modified iron oxide magnetic nanoparticles (F@AAs@Chrysin NPs), it was characterized by XRD, TGA, FTIR, VSM and TEM techniques. Finally, MTT assays on HFF-2 and HEK-293 cell lines were performed for determination of biocompatibility of AA coated IONPs...
October 15, 2018: Pharmaceutical Development and Technology
Li Ding, Xiang Gao, Jianlei Hu, Shenghui Yu
(‑)Epigallocatechin‑3‑gallate (EGCG) is a type of polyphenol monomer and is the predominant component of catechin compounds extractable from green tea. Previous studies have demonstrated that EGCG exhibits numerous bioactivities both in vitro and in vivo, including antitumor, antioxidant and anti‑inflammatory activities, as well as lowering blood lipid levels and protecting against radiation. The present study aimed to investigate whether administration of EGCG may attenuate anesthesia‑induced memory deficit in young mice and to reveal the associated underlying mechanisms...
October 10, 2018: Molecular Medicine Reports
Katharina Waldbauer, Günter Seiringer, Christina Sykora, Verena M Dirsch, Martin Zehl, Brigitte Kopp
Pomace, the press residue from different fruits accumulating as waste product in food industry, contains high amounts of secondary metabolites that could be utilized for health-related applications. This study aims at evaluating the potential of pomaces of apricot, bilberry, and elderberry to serve as a source for endothelial nitric oxide synthase (eNOS)-activating compounds. Five extracts obtained from the lyophilized pomace of apricot and elderberry with solvents of different polarity were found to enhance A23187-stimulated eNOS activity when tested at 50 μg/mL in an [14 C]-l-arginine to [14 C]-l-citrulline conversion assay in the human endothelium-derived cell line EA...
September 30, 2018: ACS Omega
Yudong Lu, Yisheng Lin, Zuci Zheng, Xiaoqiong Tang, Jinyong Lin, Xiujie Liu, Mengmeng Liu, Guannan Chen, Sufang Qiu, Ting Zhou, Yao Lin, Shangyuan Feng
Surface-enhanced Raman spectroscopy (SERS) was developed here for the non-invasive detection of the hepatitis B virus (HBV). Chronic hepatitis B virus (HBV) infection is a primary health problem in the world and may further develop into cirrhosis and hepatocellular carcinoma (HCC). SERS measurement was applied to two groups of serum samples. One group included 93 HBV patients and the other group included 94 healthy volunteers as control subjects. Tentative assignments of the Raman bands in the measured SERS spectra have shown the difference of the serum SERS spectra between HBV patients and healthy volunteers...
October 1, 2018: Biomedical Optics Express
Daniela Gentile, Matteo Fornai, Carolina Pellegrini, Rocchina Colucci, Laura Benvenuti, Emiliano Duranti, Stefano Masi, Sara Carpi, Paola Nieri, Anna Nericcio, Francesca Garelli, Agostino Virdis, Laura Pistelli, Corrado Blandizzi, Luca Antonioli
Purpose: Luteolin exerts beneficial effects against obesity-associated comorbidities, although its influence on vascular dysfunction remains undetermined. We examined the effects of luteolin on endothelial dysfunction in a mouse model of diet-induced obesity. Methods: Standard diet (SD) or high-fat diet (HFD)-fed mice were treated daily with luteolin intragastrically. After 8 weeks, body and epididymal fat weight, as well as blood cholesterol, glucose, and triglycerides were evaluated. Endothelium-dependent relaxations of resistance mesenteric vessels was assessed by a concentration-response curve to acetylcholine, repeated upon Nw -nitro-L-arginine methylester (L-NAME) or ascorbic acid infusion to investigate the influence of nitric oxide (NO) availability and reactive oxygen species (ROS) on endothelial function, respectively...
2018: Frontiers in Pharmacology
Akram Assali, Omid Akhavan, Fatemeh Mottaghitalab, Mohsen Adeli, Rassoul Dinarvand, Shayan Razzazan, Ehsan Arefian, Masoud Soleimani, Fatemeh Atyabi
Introduction: MicroRNA-101 (miR-101) is an intense cancer suppressor with special algorithm to target a wide range of pathways and genes which indicates the ability to regulate apoptosis, cellular stress, metastasis, autophagy, and tumor growth. Silencing of some genes such as Stathmin1 with miR-101 can be interpreted as apoptotic accelerator and autophagy suppressor. It is hypothesized that hybrid microRNA (miRNA) delivery structures based on cationized graphene oxide (GO) could take superiority of targeting and photothermal therapy to suppress the cancer cells...
2018: International Journal of Nanomedicine
Youakim Saliba, Victor Jebara, Joelle Hajal, Richard Maroun, Stéphanie Chacar, Viviane Smayra, Joel Abramowitz, Lutz Birnbaumer, Nassim Fares
AIMS: Cardiac fibroblasts (CFs) are emerging as major contributors to myocardial fibrosis (MF), a final common pathway of many etiologies of heart disease. Here, we studied the functional relevance of transient receptor potential canonical 3 (TRPC3) channels and nuclear factor of activated T-cells c3 (NFATc3) signaling in rodent and human ventricular CFs, and whether their modulation would limit MF. RESULTS: A positive-feedback loop between TRPC3 and NFATc3 drove a rat ventricular CF fibrotic phenotype...
October 15, 2018: Antioxidants & Redox Signaling
Nicolò Mauro, Giulia Di Prima, Paola Varvarà, Mariano Licciardi, Gennara Cavallaro, Gaetano Giammona
Chitosan oligosaccharide (C) was functionalized with L-arginine (A) and short hydrocarbon chains (C8 ) to design an amphiphilic copolymer, henceforth CAC8 , leading to microparticles consisting of an arginine-decorated hydrophilic shell and inner hydrophobic domains allowing the encapsulation of high amount hydrophobic drugs such as sorafenib tosylate (> 10 % w/w). L-arginine side chains were selected in order to impart the final microparticles enhanced transcorneal penetration properties, thus overcoming the typical biological barriers which hamper the absorption of drugs upon topical ocular administration...
October 9, 2018: Journal of Pharmaceutical Sciences
Alexander Bollenbach, Erik Hanff, Dimitrios Tsikas
NG ,NG -Dimethyl-L-arginine (asymmetric dimethylarginine, ADMA) is a cardiovascular risk factor. ADMA circulates in blood as a free acid (fADMA) and as constituent of not yet identified proteins (prADMA). We describe here a protocol for the GC-MS quantification of ADMA released from serum proteins using 6 M HCl (110 °C, 20 h). L-Homoarginine (hArg) is useful in measuring digestibility of amino acids in food proteins. We demonstrate that hArg is not present in human serum proteins and is useful in measuring serum prADMA...
October 9, 2018: Analytical Biochemistry
Noriyasu Kamei, Hideyuki Tamiwa, Mari Miyata, Yuta Haruna, Koyo Matsumura, Hideyuki Ogino, Serena Hirano, Kazuhiro Higashiyama, Mariko Takeda-Morishita
Cell-penetrating peptides (CPPs) have great potential to efficiently deliver drug cargos across cell membranes without cytotoxicity. Cationic arginine and hydrophobic tryptophan have been reported to be key component amino acids for cellular internalization of CPPs. We recently found that l-arginine could increase the oral delivery of insulin in its single amino acid form. Therefore, in the present study, we evaluated the ability of another key amino acid, tryptophan, to enhance the intestinal absorption of biopharmaceuticals...
October 10, 2018: Pharmaceutics
Manoel Miranda Neto, Luciana Tavares Toscano, Fabiano Ferreira de Lima, Taís Feitosa Da Silva, Cássia Surama Oliveira Da Silva, Alexandre Sérgio Silva
OBJECTIVE: to verify if one single dose of L-arginine improves post-exercise hypotension (PEH) in hypertensive. METHODS: double-blind, placebo, randomized with 20 hypertensive (51.47 ± 1.24 years). Two sessions of aerobic exercise were performed proceeded for the ingestion of one dose of 7 g of L-arginine (EX-LARG) or placebo (EX-PLA), plus one session only with L-arginine ingestion (L-ARG). Blood pressure (BP) was measured at rest, and each ten minutes for a period of 60 minutes recovery after exercise...
October 8, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
Marta Toral, Rosario Jimenez, Sebastián Montoro-Molina, Miguel Romero, Rosemary Wangensteen, Juan Duarte, Félix Vargas
Thyroid hormone activity is associated with L-arginine metabolism and nitric oxide (NO) production, which participate in the cardiovascular manifestations of thyroid disorders. L-arginine transporters play an important role in activating L-arginine uptake and NO production. However, the effects of thyroid hormones on L-arginine transporters in endothelial cells have not yet been evaluated. The following methods were used. We measured L-arginine uptake, mRNA expression of L-arginine transporters, endothelial nitric oxide synthase (eNOS) mRNA and NO generation after the administration of T3, T4 and the T3 analog, 3,3′,5-triiodothyroacetic acid TRIAC in human umbilical vein endothelial cells (HUVECs)...
October 1, 2018: Journal of Endocrinology
Qi Zhang, Jian Zhang, Song Xue, Mengjie Rui, Bin Gao, Ang Li, Jiashuai Bai, Zhichao Yin, Anochie Ebubedike Mitchelle
In the cyclodextrins family, the native α-cyclodextrin has almost been abandoned in CE chiral separation due to its much weaker enantioselectivity compared with β-cyclodextrin and their derivatives. In this work, several amino acid chiral ionic liquids were selected to establish synergistic enantioseparation systems with native α-cyclodextrin. Enhanced enantioselectivities were observed in the chiral ionic liquids/α-cyclodextrin synergistic systems compared with single α-cyclodextrin system. A series of comparison experiments were performed to demonstrate the superiority of the synergistic systems...
October 11, 2018: Journal of Separation Science
A K M Cavalcante, R C L Siqueira, V N Feitosa Júnior, C R de Andrade, A A Santos, M T B Silva
We previously found that acute exercise inhibited the gastric emptying of liquid in awake rats by causing an acid-base imbalance. In the present study, we investigated the involvement of the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) pathway, vasoactive intestinal peptide (VIP), and corticotropin-releasing factor (CRF) peptide in this phenomenon. Male rats were divided into exercise or sedentary group and were subjected to a 15-min swim session against a load (2.5 or 5% b.w.). The rate of gastric emptying was evaluated after 5, 10, or 20 min postprandially...
October 4, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Yuki Izawa-Ishizawa, Masaki Imanishi, Yoshito Zamami, Hiroki Toya, Tomoko Nagao, Marin Morishita, Koichi Tsuneyama, Yuya Horinouchi, Yoshitaka Kihira, Kenshi Takechi, Yasumasa Ikeda, Koichiro Tsuchiya, Masanori Yoshizumi, Toshiaki Tamaki, Keisuke Ishizawa
OBJECTIVE: Aortic dissection is a life-threatening disease. At present, the only therapeutic strategies available are surgery and antihypertensive drugs. Moreover, the molecular mechanisms underlying the onset of aortic dissection are still unclear. We established a novel aortic dissection model in mice using pharmacologically induced endothelial dysfunction. We then used the Japanese Adverse Drug Event Report database to investigate the role of pitavastatin in preventing the onset of aortic dissection...
July 24, 2018: Journal of Hypertension
Lin Yan, Qincai Tang, Xiaojing Quan, Haixia Ren, Wei Chen, Hong Xia, Hesheng Luo
BACKGROUND: Glucagon-like peptide-1 (GLP-1) receptor (GLP-1R) agonists modulate gastrointestinal motility; however, the effects of GLP-1R agonists on colonic motility are still controversial, and the molecular mechanism is unclear. Exendin-4 shares 53% homology with GLP-1 and is a full agonist of GLP-1R. In this study, our aims were to explore the role and mechanism of exendin-4 in isolated rat colonic tissues and cells. METHODS: An organ bath system was used to examine the spontaneous contractions of smooth muscle strips...
October 10, 2018: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
Ariandra G Sartim, Bianca M Brito, Pedro H Gobira, Sâmia R L Joca
The transient receptor potential vanilloid 1 (TRPV1) can modulate stress-related behaviours, thus representing an interesting target for new antidepressant drugs. TRPV1 can trigger glutamate release and nitric oxide synthesis in the brain, mechanisms also involved in the neurobiology of depression. However, it is not known if these mechanisms are involved in TRPV1-induced behavioural effects. Therefore, the aim of this study was to verify if the antidepressant-like effect induced by a TRPV1 antagonist in mice submitted to the forced swimming test (FST) would be facilitated by combined treatment with neuronal nitric oxide synthase (nNOS) inhibition and N-methyl-D-aspartate (NMDA) blockade...
October 5, 2018: Behavioural Pharmacology
M Nascimento, R Piran, R M Da Costa, M A Giordani, F S Carneiro, D H Aguiar, M C Dias, M M Sugizaki, R A Luvizotto, A F Nascimento, G F Bomfim
Liver cirrhosis is associated with a wide range of cardiovascular abnormalities including hyperdynamic circulation and cirrhotic cardiomyopathy. The pathogenic mechanisms of these cardiovascular changes are multifactorial and include vascular dysregulations. AIM: The present study tested the hypothesis that the systemic vascular hyporesponsiveness in thioacetamide (TAA)-induced liver injury model is dependent on nitric oxide (NO) and cyclooxygenase (COX) derivatives. MAIN METHODS: Wistar rats were treated with TAA for eight weeks to induce liver injury...
October 4, 2018: Life Sciences
Vaibhav Walia, Chanchal Garg, Munish Garg
Glutamate is an excitatory neurotransmitter implicated in the pathogenesis of psychiatric disorders. Glutamate results in the activation of an enzyme called glycogen synthase kinase-3 (GSK-3) acting through N-methyl-D-aspartate (NMDA) receptors. Impaired expression of GSK-3 affects behavior and neurochemicals level in the brain responsible for the pathogenesis of mood disorders. It has been reported that lithium acts as an inhibitor of GSK-3 and inhibit the enzyme GSK-3 in an uncompetitive manner. In the present study, anxiolytic like effect of lithium in mice is investigated through light and dark box (LDB) and elevated pus maze (EPM)...
October 4, 2018: Brain Research
Baruh Polis, Kolluru D Srikanth, Evan Elliott, Hava Gil-Henn, Abraham O Samson
The urea cycle is strongly implicated in the pathogenesis of Alzheimer's disease (AD). Arginase-I (ARGI) accumulation at sites of amyloid-beta (Aβ) deposition is associated with L-arginine deprivation and neurodegeneration. An interaction between the arginase II (ARGII) and mTOR-ribosomal protein S6 kinase β-1 (S6K1) pathways promotes inflammation and oxidative stress. In this study, we treated triple-transgenic (3×Tg) mice exhibiting increased S6K1 activity and wild-type (WT) mice with L-norvaline, which inhibits both arginase and S6K1...
October 4, 2018: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
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