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Tran T Q Lan, Marie-Lou Gaucher, Nguyen T M Nhan, Ann Letellier, Sylvain Quessy
This study was carried out to evaluate the prevalence of Salmonella serogroups and serotypes and their virulence gene carriage in pig fecal samples from farms and slaughterhouses in some southern provinces of Vietnam. The presence of Salmonella was assessed based on culture enrichment of the collected samples and biochemical and serological analyses; 27.7% (51) of 184 samples were posititve for Salmonella. Based on the availability of antisera, serogroups were determined for 61% (31) of 51 isolates. Twenty isolates belonging to Salmonella serotypes Typhimurium (10 isolates), Anatum (8 isolates), Senftenberg (7 isolates), Paratyphi B (3 isolates), Paratyphi A (1 isolate), Montevideo (1 isolate), and Saintpaul (1 isolate) were further characterized by a multiplex PCR protocol targeting Salmonella invasion A and virulence plasmid C genes ( invA and spvC, respectively)...
August 7, 2018: Journal of Food Protection
Tom S Edrington, J Armando Garcia Buitrago, G Robert Hagevoort, G H Loneragan, D M Bricta-Harhay, Todd R Callaway, Robin C Anderson, David J Nisbet
The objective of the current research was to determine if pasteurization of nonsaleable waste milk influences fecal Salmonella concentrations and prevalence, or antimicrobial susceptibility and serotype of the cultured isolates. Holstein dairy calves (n = 211) were housed on a single commercial dairy in the southwestern United States and randomly allotted to be fed either pasteurized (PWM; n = 128 calves) or nonpasteurized waste milk (NPWM; n = 83 calves). Fecal samples were collected via rectal palpation or from freshly voided, undisturbed fecal pats, weekly during the first 4 wk of the animal's life and then again at weaning...
August 1, 2018: Journal of Dairy Science
Ignacio Estevan, Ana Silva, Bettina Tassino
Morningness-Eveningness (M-E) has been associated with school performance when school activities are arranged early in the morning. This study aims to evaluate this association in 224 adolescents of a secondary school in Montevideo, Uruguay, attending either morning or afternoon shifts. Students' socio-demographic characteristics and performance indicators were similar across shifts, while afternoon-shift students exhibited later circadian preferences. After controlling for socio-demographic and other educational variables, eveningness was associated with lower grades only in morning-shift students...
August 1, 2018: Chronobiology International
Scott V Nguyen, Dayna M Harhay, James L Bono, Timothy P L Smith, Patricia I Fields, Blake A Dinsmore, Monica Santovenia, Rong Wang, Joseph M Bosilevac, Gregory P Harhay
Salmonella enterica serovar Montevideo has been linked to recent foodborne illness outbreaks resulting from contamination of products such as fruits, vegetables, seeds and spices. Studies have shown that Montevideo also is frequently associated with healthy cattle and can be isolated from ground beef, yet human salmonellosis outbreaks of Montevideo associated with ground beef contamination are rare. This disparity fuelled our interest in characterizing the genomic differences between Montevideo strains isolated from healthy cattle and beef products, and those isolated from human patients and outbreak sources...
July 27, 2018: Microbial Genomics
Govindaraj Dev Kumar, Robert C Williams, Nammalwar Sriranganathan, Renee R Boyer, Joseph D Eifert
Salmonella serotypes linked to tomato-associated outbreaks were evaluated for survival in soil and water over a 40-day period. Salmonella enterica serotypes Anatum, Baildon, Braenderup, Montevideo, Newport, and Javiana were inoculated separately into sterile soil and water, followed by plating onto TSAYE and XLT4 at 10-day intervals. Biofilm production by Salmonella serotypes was measured on both quartz particles (soil surrogate) and glass coverslips, and was evaluated using a crystal violet dye assay. Salmonella populations in soil and water over 40 days indicated no significant differences between Salmonella serotypes tested (p > 0...
July 16, 2018: Foodborne Pathogens and Disease
Katarzyna Kordas, Aditi Roy, Marie Vahter, Julia Ravenscroft, Nelly Mañay, Fabiana Peregalli, Gabriela Martínez, Elena I Queirolo
Oxidative stress (OS) is an important consequence of exposure to toxic metals but it is unclear to what extent low-level metal exposures contribute to OS in children. We examined the cross-sectional association between urinary concentrations of arsenic (As), cadmium (Cd), and lead (Pb) and urinary markers of OS: F2 -8α isoprostane and 8-hydroxy-2-deoxy-guanosine (8-OHdG). We also tested effect modification by dietary intakes. Of the 211 children aged 6-8 years living in Montevideo who were eligible for the study because they had at least one OS marker measured via ELISA, 143 were included in a complete-case analysis...
June 26, 2018: Environmental Research
Julie Haendiges, Tyann Blessington, Jie Zheng, Gordon Davidson, Jesse D Miller, Maria Hoffmann
A multistate outbreak of 11 Salmonella infections linked to pistachio nuts occurred in 2016. In this announcement, we report the complete genome sequences of four Salmonella enterica subsp. enterica serovar Senftenberg and S. enterica subsp. enterica serovar Montevideo isolates from pistachios collected during the 2016 outbreak investigation.
June 28, 2018: Genome Announcements
Caroline S Silveira, Deborah Cesar, M Kelly Keating, Marlene DeLeon-Carnes, Aníbal G Armién, Martín Luhers, Franklin Riet-Correa, Federico Giannitti
Protothecosis is a rare disease caused by environmental algae of the genus Prototheca. These are saprophytic, non-photosynthetic, aerobic, colorless algae that belong to the Chlorellaceae family. Seven different species have been described. Prototheca zopfii genotype 2 and P. wickerhamii are most commonly involved in pathogenic infections in humans and animals. The objective of this work is to describe, for the first time, a case of protothecosis caused by P. zopfii genotype 1 in a dog. The dog, a 4-year-old mix bred male, was presented to a veterinary clinic in Montevideo, Uruguay, with multiple skin nodules, one of which was excised by surgical biopsy...
June 6, 2018: Mycopathologia
Alyssa Zackler, Pamela Flood, Rori Dajao, Lauren Maramara, Laura Goetzl
OBJECTIVE: Chorioamnionitis is associated with an increased risk of cesarean delivery and uterine atony. We hypothesized that the onset of maternal fever is temporally associated with decreased uterine contractility. STUDY DESIGN: Retrospective cohort. SETTING: Academic center. PATIENTS: Term participants who developed a fever in the setting of an intrauterine pressure catheter. MAIN OUTCOME MEASURE: Montevideo units (MVUs) and oxytocin dose at time 0 (first oral temperature ≥38°C) and in the five 1--hour blocks preceding and following T0...
January 1, 2018: Reproductive Sciences
Xuetong Fan, Joshua B Gurtler, Kimberly J B Sokorai
A study was conducted to evaluate the effects of post-treatment rinsing with water on the inactivation efficacy of acid treatments against Salmonella inoculated onto stem scar areas of two types of tomatoes. In addition, impact on fruit quality was investigated during 21 days post-treatment storage at 10 °C. A four-strain cocktail of Salmonella enterica (S. Montevideo, S. Newport, S. Saintpaul, and S. Typhimurium) was inoculated onto stem scar areas of grape and large round tomatoes. The inoculated fruits were then treated for 2 min with the following solutions: water, 2% lactic acid +2% acetic acid +2% levulinic acid, 1...
September 2, 2018: International Journal of Food Microbiology
Piumi De Abrew Abeysundara, Nitin Dhowlaghar, Ramakrishna Nannapaneni, Mark W Schilling, Barakat Mahmoud, Chander S Sharma, Din-Pow Ma
Salmonella enterica is responsible for the highest number of foodborne disease outbreaks pertaining to cantaloupe industry. The objective of this study was to examine the growth and biofilm formation by outbreak strains of S. enterica ser. Poona (S. Poona), S. enterica ser. Stanley (S. Stanley) and S. enterica ser. Montevideo (S. Montevideo) on different food-contact processing surfaces in cantaloupe flesh and peel extracts at 22 °C and 10 °C. The generation time of all S. enterica strains tested was shorter in the high concentration (50 mg/ml) of cantaloupe extract and high temperature...
September 2, 2018: International Journal of Food Microbiology
Saranya Nair, Abdolvahab Farzan, Terry L O'Sullivan, Robert M Friendship
A longitudinal trial was conducted to determine the course of Salmonella shedding and antibody response in naturally infected grower-finisher pigs. Ten-week-old pigs ( n = 45) were transferred from a farm with history of salmonellosis and housed at a research facility. Weekly fecal samples (weeks 1 to 11) as well as tissue samples at slaughter were cultured for Salmonella. Serum samples were tested for presence of Salmonella antibody by enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a multilevel mixed-effects logistic regression model...
April 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Leandro Machín, Jessica Aschemann-Witzel, María Rosa Curutchet, Ana Giménez, Gastón Ares
OBJECTIVE: To evaluate how information about low nutrient content included in the traffic light labeling system influences consumers' perception of the healthfulness of products with high content of 1 key nutrient, and to compare the traffic light system with warnings in terms of the perception of healthfulness. DESIGN: Images of front-of-pack (FOP) nutrition labels (the traffic light labeling system with different numbers of nutrients with low content, and warnings) were evaluated in study 1, whereas product labels featuring the different FOP nutrition labels were evaluated in study 2...
July 2018: Journal of Nutrition Education and Behavior
Jianfa Bai, Valentina Trinetta, Xiaorong Shi, Lance W Noll, Gabriela Magossi, Wanglong Zheng, Elizabeth P Porter, Natalia Cernicchiaro, David G Renter, Tiruvoor G Nagaraja
Cattle lymph nodes can harbor Salmonella and potentially contaminate beef products. We have developed and validated a new real-time PCR (qPCR) assay for the detection and quantification of Salmonella enterica in cattle lymph nodes. The assay targets both the invA and pagC genes, the most conserved molecular targets in Salmonella enterica. An 18S rRNA gene assay that amplifies from cattle and other animal species was also included as an internal control. Available DNA sequences for invA, pagC and 18S rRNA genes were used for primer and probe selections...
May 2018: Journal of Microbiological Methods
Esmeralda Rangel-Vargas, Anais M Luna-Rojo, Arturo Cadena-Ramírez, Refugio Torres-Vitela, Carlos A Gómez-Aldapa, Angélica Villarruel-López, Alejandro Téllez-Jurado, José R Villagómez-Ibarra, Rosalía Reynoso-Camacho, Javier Castro-Rosas
The behavior of foodborne bacteria on whole and cut mangoes and the antibacterial effect of Hibiscus sabdariffa calyx extracts and chemical sanitizers against foodborne bacteria on contaminated mangoes were investigated. Mangoes var. Ataulfo and Kent were used in the study. Mangoes were inoculated with Listeria monocytogenes, Shigella flexneri, Salmonella Typhimurium, Salmonella Typhi, Salmonella Montevideo, Escherichia coli strains (O157:H7, non-O157:H7 Shiga toxin-producing, enteropathogenic, enterotoxigenic, enteroinvasive, and enteroaggregative)...
May 2018: Journal of Food Protection
Víctor-Guillermo Sequera, Marisa Mena, Malen Hollmann, Estefani Mani, Viviana Ramas, Sylvia Bonilla, Alicia Guerra, Fernando Borgia
OBJECTIVES: To identify factors associated with high-risk human papillomavirus (HPV) infection and high grade squamous intraepithelial lesion (HSIL) among a high-risk group of HPV-unvaccinated women in Montevideo. METHODS: Participants completed a questionnaire on socio-demographics, sexual behavior and gynecological history and received a gynecological examination. HPV DNA was detected by PCR using MY09/11 primers. Logistic regression analyses were performed to identify factors associated with high-risk HPV infection and HSIL...
June 2018: Papillomavirus Research
P F Amorim
The Jenynsia lineata species complex comprises J. lineata from Montevideo, Uruguay and Jenynsia multidentata, from coastal basins of Brazil, Uruguay and Argentina up to 1200 m a.s.l. Taxonomic divisions within this group were tested using three different species delimitation methods, which found the two existing names to be synonyms and revealed a new cryptic species. Jenynsia darwini sp. nov. is distinguished from all congeners by having a unique combination of character states, including the shape of the dorsal postcleithrum (three times higher than wide v...
May 2018: Journal of Fish Biology
Marília Leão Goettems, Mariana Ourens, Laura Cosetti, Susana Lorenzo, Ramon Álvarez-Vaz, Roger Keller Celeste
This study aims to estimate orthodontic treatment need among 15-24 year-old individuals in Montevideo, Uruguay, and the association of occlusal traits with demographic, clinical and socioeconomic factors, considering a life course approach. A cross-sectional study using data from the First National Oral Health Survey in Uruguay was conducted. A two-stage cluster procedure was used to select a sample of 278 individuals in Montevideo. Household interviews and oral examinations were performed by six dentists. Dental Aesthetic (DAI) and Decayed Missing and Filled Teeth Indices (DMFT) were used to assess orthodontic treatment need and dental caries, respectively...
March 5, 2018: Cadernos de Saúde Pública
Gauri Desai, Gabriel Barg, Elena I Queirolo, Marie Vahter, Fabiana Peregalli, Nelly Mañay, Katarzyna Kordas
BACKGROUND: Few studies have evaluated the association between low-level arsenic (As) exposure and cognitive performance among children. OBJECTIVES: In this cross-sectional study, we assessed the association between low-level As exposure and cognitive performance among 5-8 year-old children in Montevideo, and tested effect modification by As methylation capacity and children's dietary folate intake. METHODS: We measured total urinary As (UAs) concentrations and the proportion of monomethylarsonic acid (MMA) in the urine of 328 children...
July 2018: Environmental Research
Victor A Amadi, Harry Hariharan, Gitanjali Arya, Vanessa Matthew-Belmar, Roxanne Nicholas-Thomas, Rhonda Pinckney, Ravindra Sharma, Roger Johnson
Non-typhoidal salmonellosis remains an important public health problem worldwide. Dogs may harbour Salmonella in their intestines and can easily shed Salmonella in the environment with the possibility of transmission to humans. Thus, monitoring is essential to understand the role of dogs in zoonotic transmission. The objectives of this study were to determine the shedding of Salmonella by owned, apparently healthy dogs in Grenada, West Indies, to identify the serovars, and to examine their antimicrobial susceptibility profiles...
February 2018: Veterinary Medicine and Science
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