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follicular development

Xiao-Gang Weng, Yan Liu, Si-Han Zhou, Yu-Ting Zhang, Yu-Tong Shao, Qian-Qian Xu, Zhong-Hua Liu
In vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) are important breeding techniques for livestock. High-quality MII oocytes produced from in vitro maturation (IVM) are required for the two techniques listed above. The ovaries used for IVM operations are primarily acquired from commercial abattoirs, and the pathogen status of slaughtered animals becomes an unavoidable issue. Our previous monitoring data showed that porcine circovirus type 2 (PCV-2) is the main pathogen present in ovaries from abattoirs...
December 4, 2018: Theriogenology
Elena V Fedorova, Natalja V Dorogova, Elena U Bolobolova, Svetlana A Fedorova, Dmitry A Karagodin, Anna A Ogienko, Asja S Khruscheva, Elina M Baricheva
Investigation of Drosophila oogenesis provides the opportunity to understand conservative genetic mechanisms underlying fertile female gamete development. In this work, we showed that the Drosophila DNA-binding protein GAGA factor (GAF) had a multifunctional role in oogenesis and it is involved in the regulation of this process genetic program. We studied the influence on Drosophila oogenesis of a number of mutations in the 5' region of the Trl gene that encodes GAF. We found that our originally generated Trl mutations lead to a decrease in transcriptional gene activity and levels of GAF expression in both germline and follicular cells...
December 10, 2018: Genesis: the Journal of Genetics and Development
Haoran Shen, Yao Wang
OBJECTIVE: To investigate the role of the transforming growth factor-β1 (TGF-β1)/Smad3 signaling pathway in development of ovarian follicle by promoting apoptosis of granulosa cells in polycystic ovary syndrome (PCOS). METHODS: A total of 54 female rats were obtained and randomly assigned into the PCOS (n = 27) and control groups ( n = 27). PCOS rat models were constructed using the dehydroepi-androsterone method to observe ovarian morphology and ultrastructure...
December 7, 2018: Journal of Cellular Physiology
Chloe Broughton, Jane Mears, Adam Williams, Kathryn Lonnen
Pituitary adenomas can be classified as functioning or non-functioning adenomas. Approximately 64% of clinically non-functioning pituitary adenomas are found to be gonadotroph adenomas on immunohistochemistry. There are reported cases of gonadotroph adenomas causing clinical symptoms, but this is unusual. We present the case of a 36-year-old female with abdominal pain. Multiple large ovarian cysts were identified on ultrasound requiring bilateral cystectomy. Despite this, the cysts recurred resulting in further abdominal pain, ovarian torsion and right oophorectomy and salpingectomy...
December 11, 2018: Endocrinology, Diabetes & Metabolism Case Reports
S Wohlres-Viana, E K N Arashiro, T P Minare, C A C Fernandes, J G V Grazia, L G B Siqueira, M A Machado, J H M Viana
The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH...
December 3, 2018: Theriogenology
M Saeed Qureshi, Jawaher Alsughayyir, Manu Chhabra, Jason M Ali, Martin J Goddard, Chris Devine, Thomas M Conlon, Michelle A Linterman, Reza Motallebzadeh, Gavin J Pettigrew
The development of humoral autoimmunity following organ transplantation is increasingly recognised, but of uncertain significance. We examine whether autoimmunity contributes independently to allograft rejection. In a MHC class II-mismatched murine model of chronic humoral rejection, we report that effector antinuclear autoantibody responses were initiated upon graft-versus-host allorecognition of recipient B cells by donor CD4 T-cells transferred within heart allografts. Consequently, grafts were rejected more rapidly, and with markedly augmented autoantibody responses, upon transplantation of hearts from donors previously primed against recipient...
December 6, 2018: Journal of Autoimmunity
Wei Wang, Shi-Ming Luo, Jun-Yu Ma, Wei Shen, Shen Yin
Organophosphorus insecticide diazinon (DZN) is diffusely used in agriculture, home gardening and crop peats. Much work so far has focused on the link between DZN exposure and the occurrence of neurological diseases, while, it is little-known that reproductive toxicological assessment on DZN exposure. This research aimed to investigate the underlying mechanisms of toxic hazard for DZN exposure on the cultured porcine ovarian granulosa cells. We analyzed the oxidative stress, energy metabolism, DNA damage, apoptosis and autophagy by using high-throughput RNA-seq, immunofluorescence, western blotting and real-time PCR...
December 11, 2018: Journal of Agricultural and Food Chemistry
Kevin W Ng, Erin A Marshall, Katey Ss Enfield, Spencer D Martin, Katy Milne, Michelle E Pewarchuk, Ninan Abraham, Wan L Lam
T follicular helper cells (Tfh) play crucial roles in the development of humoral immunity. In the B cell-rich germinal center of lymphoid organs, they select for high-affinity B cells and aid in their maturation. While Tfh have known roles in B cell malignancies and have prognostic value in some epithelial cancers, their role in lung tumour initiation and development is unknown. Through immune cell deconvolution, we observed significantly increased Tfh in tumours from two independent cohorts of lung adenocarcinomas and found that this upregulation occurs early in tumour development...
2018: Oncoimmunology
Brodie Miles, Elizabeth Connick
Follicular regulatory T cells (Tfr) are a unique subset of CD4 T cells that control and impact adaptive immune responses in the lymphoid follicles and germinal centers (GC). Since their relatively recent discovery, several studies have revealed that Tfr interact with other cells within this niche and shape ensuing responses. Recent advances defining the functional and developmental characteristics of Tfr have revealed key characteristics of Tfr differentiation, GC recruitment and retention, and regulatory properties...
2018: Frontiers in Immunology
Daisuke Ennishi, Aixiang Jiang, Merrill Boyle, Brett Collinge, Bruno M Grande, Susana Ben-Neriah, Christopher Rushton, Jeffrey Tang, Nicole Thomas, Graham W Slack, Pedro Farinha, Katsuyoshi Takata, Tomoko Miyata-Takata, Jeffrey Craig, Anja Mottok, Barbara Meissner, Saeed Saberi, Ali Bashashati, Diego Villa, Kerry J Savage, Laurie H Sehn, Robert Kridel, Andrew J Mungall, Marco A Marra, Sohrab P Shah, Christian Steidl, Joseph M Connors, Randy D Gascoyne, Ryan D Morin, David W Scott
PURPOSE: High-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements (HGBL-DH/TH) has a poor outcome after standard chemoimmunotherapy. We sought to understand the biologic underpinnings of HGBL-DH/TH with BCL2 rearrangements (HGBL-DH/TH- BCL2) and diffuse large B-cell lymphoma (DLBCL) morphology through examination of gene expression. PATIENTS AND METHODS: We analyzed RNA sequencing data from 157 de novo germinal center B-cell-like (GCB)-DLBCLs, including 25 with HGBL-DH/TH- BCL2, to define a gene expression signature that distinguishes HGBL-DH/TH- BCL2 from other GCB-DLBCLs...
December 3, 2018: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
Daolun Yu, Li Zhang, Hejian Wang, Fanghui Chen, Jie Chen, Zongmeng Zhang, Jie Li, Chaofeng Xing, Honglin Li, Jun Li, Yafei Cai
The egg production of poultry depends on follicular development and selection. Nonetheless, the mechanism underlying the priority of selecting of hierarchical follicles is completely unknown. SMAD9 is one of the important transcription factors in the BMP/SMAD pathway and is involved in goose follicular initiation. To identify its potential role in determination of the goose follicle hierarchy, we used BMP type I receptor inhibitor LDN-193189 both in vivo and in vitro and found that SMAD9 mRNA expression decreased in the presence of LDN-193189...
November 22, 2018: Theriogenology
Shirdi E Schmiel, Lokesh A Kalekar, Na Zhang, Thomas W Blankespoor, Londyn J Robinson, Daniel L Mueller
OBJECTIVE: CD4 germinal center (GC)-T follicular helper (Tfh) cells are important in the pathogenesis of autoimmune arthritis. Previous studies have shown that adenosine 2a receptor (A2aR, Adora2a) signaling can divert CD4 T cells away from the GC-Tfh cell lineage during the primary response to foreign antigens. Therefore, we examined the effects of A2aR signaling on CD4 T cells during the recognition of a self-antigen using a mouse model of autoimmune arthritis. METHODS: Wild type and Adora2a-deficient KRN TCR-transgenic CD4 T cells specific for glucose-6-phosphate isomerase (GPI)/I-Ag7 were transferred into immunodeficient Tcra-/- I-Ag7 -expressing mice to induce arthritis...
December 5, 2018: Arthritis & Rheumatology
Karin Hohloch, Christiane Windemuth-Kieselbach, Julia Kolz, Pier Luigi Zinzani, Roberto Cacchione, Woijciech Jurczak, Angelika Bischof Delaloye, Lorenz Trümper, Christian W Scholz
To assess efficacy of radioimmunotherapy (RIT) in follicular lymphoma, data from 281 patients collected in the RIT Network, with a median follow-up of 8·2 years after RIT were analysed. RIT was given at first line in 18·5% and at relapse in 81·5%. Following first line therapy, 76·9% achieved complete remission (CR), 9·6% partial remission (PR), 1·9% stable disease (SD) and 1·9% had progressive disease (PD); response was not documented in 9·7%. At relapse, the rate of CR was 48·5% and that of PR was 16·6%, SD 2·6% and PD 10·5%; response was not documented in 21·8%...
December 4, 2018: British Journal of Haematology
Anusara Daenthanasanmak, Supinya Iamsawat, Paramita Chakraborty, Hung D Nguyen, David Bastian, Chen Liu, Shikhar Mehrotra, Xue-Zhong Yu
Graft-versus-host-disease (GVHD) remains one of the major complications after allogeneic bone marrow transplantation (allo-BMT). Sirtuin-1 (Sirt-1) plays a crucial role in various biological processes including cellular senescence, metabolism and inflammatory responses. Sirt-1 deacetylation regulates different transcription factors that are important for modulating immune responses. In the current study, we addressed the role of Sirt-1 in GVHD induction by employing Sirt-1 conditional knock-out mice as well as a pharmacological Sirt-1 inhibitor...
December 4, 2018: Blood
Yaolin Song, Ana C Boncompagni, Sang-Seok Kim, Heather R Gochnauer, Yuhang Zhang, Gabriela G Loots, Dianqing Wu, Yulin Li, Mingang Xu, Sarah E Millar
Haired skin is a defining characteristic of mammals. However, some specialized skin regions, such as human palms, soles and ventral wrist, and mouse plantar foot, are entirely hairless. Using mouse plantar skin as a model system, we show that the endogenous secreted Wnt inhibitor DKK2 suppresses plantar hair follicle development and permits the formation of hairless skin. Plantar skin retains all of the mechanistic components needed for hair follicle development, as genetic deletion of Dkk2 permits formation of fully functional plantar hair follicles that give rise to external hair, contain sebaceous glands and a stem cell compartment, and undergo regenerative growth...
November 29, 2018: Cell Reports
Leandro M Velez, Giselle A Abruzzese, María F Heber, Silvana R Ferreira, Alicia B Motta
BACKGROUND: Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear factor that may act on the early development of ovarian follicles and on follicular steroidogenesis. However, the exact mechanism of PPARG action remains unknown. We have previously found that androgen excess alters early ovarian function and the PPARG system. The aim of the present study was to evaluate whether PPARG activation (using the synthetic ligand pioglitazone (PGZ)) ameliorates the alterations in early ovarian function induced by androgen excess...
September 22, 2018: Pharmacological Reports: PR
Seema R Patel, David R Gibb, Kathryn Girard-Pierce, Xiaoxi Zhou, Lilian Cataldi Rodrigues, Connie M Arthur, Ashley L Bennett, Ryan P Jajosky, Megan Fuller, Cheryl L Maier, Patricia E Zerra, Satheesh Chonat, Nicole H Smith, Christopher A Tormey, Jeanne E Hendrickson, Sean R Stowell
Red blood cell (RBC) alloimmunization represents a significant immunological challenge for some patients. While a variety of immune constituents likely contribute to the initiation and orchestration of alloantibodies to RBC antigens, identification of key immune factors that initiate alloantibody formation may aid in the development of a therapeutic modality to minimize or prevent this process. To define the immune factors that may be important in driving alloimmunization to an RBC antigen, we determined the specific immune compartment and distinct cells that may initially engage transfused RBCs and facilitate subsequent alloimmunization...
2018: Frontiers in Immunology
Igor Govorov, Katarina Bremme, Tomas L Lindahl, Margareta Holmström, Eduard Komlichenko, Roza Chaireti, Miriam Mints
Fluctuations of the sex steroids during the menstrual cycle might significantly influence hemostasis. This association, derived from a number of the observations on healthy women, is yet to be described in females affected by bleeding disorders. The aim of the current study was to assess the changes in hemostatic variables in women with vWD during two phases of the menstrual cycle (follicular and luteal) and to compare it with healthy controls. The study group included 12 vWD-affected females with regular menstrual cycle, with none of them being prescribed any hormonal treatment...
November 30, 2018: Scientific Reports
Kauê R Martins, Cristina S Haas, Juliana G Ferst, Monique T Rovani, André L F Goetten, Raj Duggavathi, Vilceu Bordignon, Valério V M Portela, Rogério Ferreira, Paulo B D Gonçalves, Bernardo G Gasperin, T Lucia
Oncostatin M (OSM) and its receptor (OSMR) are members of the interleukin-6 family cytokines. Although OSM and OSMR expression was detected in human ovaries, their function and regulation during follicle development, ovulation and luteolysis have not been studied in any species. The aim of the present study was to investigate the levels of OSM and OSMR mRNA in bovine ovaries and the effect of OSM treatment on cultured granulosa cells. OSM mRNA was not detected in granulosa cells obtained from follicles around the time of follicular deviation and from pre-ovulatory follicles, whereas OSMR transcript levels were greater in granulosa cells of atretic subordinate follicles (P&#x202F;<&#x202F;0...
November 22, 2018: Theriogenology
Gonzalo Oubiña, Natalia Pascuali, Leopoldina Scotti, Mariana Di Pietro, Florenza A La Spina, Mariano G Buffone, Javier Higuera, Dalhia Abramovich, Fernanda Parborell
It is known that LLLT has beneficial effects on several pathological conditions including wound healing, pain and inflammation. LLLT modulates biological processes, including cell proliferation, apoptosis and angiogenesis. In the present study, we examined the effect of local application of LLLT on follicular dynamics, ovarian reserve, AMH expression, progesterone levels, apoptosis, angiogenesis, and reproductive outcome in adult mice. LLLT (200 J/cm2 ) increased the percentage of primary and preantral follicles, whilst decreasing the percentage of corpora lutea compared to control ovaries...
November 27, 2018: Progress in Biophysics and Molecular Biology
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