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Methylation tumor

Tie-Gang Meng, Xukun Lu, Lei Guo, Guan-Mei Hou, Xue-Shan Ma, Qian-Nan Li, Lin Huang, Li-Hua Fan, Zheng-Hui Zhao, Xiang-Hong Ou, Ying-Chun OuYang, Heide Schatten, Lei Li, Zhen-Bo Wang, Qing-Yuan Sun
N6-methyladenosine (m6 A) is the most prevalent and reversible internal modification of mammalian messenger and noncoding RNAs mediated by specific m6 A writer, reader, and eraser proteins. As an m6 A writer, the methyltransferase-like 3-methyltransferase-like 14 (METTL14)-Wilms tumor 1-associated protein complex dynamically regulates m6 A modification and plays important roles in diverse biologic processes. However, our knowledge about the complete functions of this RNA methyltransferase complex, the contributions of each component to the methylation, and their effects on different biologic pathways are still limited...
August 15, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Yasser Hussein Eissa Mohammed, Shaukath Ara Khanum
In modern biology, one of the major topics of importance is progress in anti-cancer drugs with specific targets. The angiopreventive and in vitro tumor inhibition activities of novel synthetic benzophenone analogs have been investigated intensively and explored in a very systematic way. Novel benzophenone analogs ( 9a-d and 10a-d ) substituted with methyl, chloro and fluoro groups at different positions on an identical chemical backbone and incorporating variations in the number of substituents have been synthesized in a multistep process and characterized...
April 1, 2018: MedChemComm
Kannika Permpoonputtana, Patlada Tangweerasing, Sujira Mukda, Parichart Boontem, Chutikorn Nopparat, Piyarat Govitrapong
Aging is often accompanied by a decline in cognitive function in conjunction with a variety of neurobiological changes, including neuroinflammation. Melatonin is a key endogenous indoleamine secreted by the pineal gland that plays a crucial role in the regulation of circadian rhythms, is a potent free radical scavenger, has anti-inflammatory activity and serves numerous other functions. However, the role of melatonin in sterile inflammation in the brain has not been fully investigated. In the present study, we investigated the neuroinflammation status in aged mouse brains...
2018: EXCLI Journal
Thomas R Pisanic, Leslie Cope, Shiou-Fu Lin, Ting-Tai Yen, Pornpat Athamanolap, Ryoichi Asaka, Kentaro Nakayama, Amanda N Fader, Tza-Huei Wang, Ie-Ming Shih, Tian-Li Wang
PURPOSE: High-grade serous ovarian carcinoma (HGSOC) typically remains undiagnosed until advanced stages when peritoneal dissemination has already occurred. Here, we sought to identify HGSOC-specific alterations in DNA methylation and assess their potential to provide sensitive and specific detection HGSOC at its earliest stages. EXPERIMENTAL DESIGN: MethylationEPIC genome-wide methylation analysis was performed on a discovery cohort comprising 23 HGSOC, 37 non-HGSOC malignant and 36 cancer-free gynecologic tissue samples...
August 14, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Mohammed Jaber, Christian Ewelt, Johannes Wölfer, Benjamin Brokinkel, Christian Thomas, Martin Hasselblatt, Oliver Grauer, Walter Stummer
BACKGROUND: Approximately 20% of low-grade gliomas (LGG) display visible protoporphyrin fluorescence during surgery after 5-aminolevulinic acid (5-ALA) administration. OBJECTIVE: To determine if fluorescence represents a prognostic marker in LGG. METHODS: Seventy-four consecutive patients with LGG (World Health Organization 2016) were operated on with 5-ALA. Fluorescent tissue was specifically biopsied. Tumor size, age, Karnofsky index, contrast-enhancement, fluorescence, and molecular factors (IDH1/IDH2-mutations, Ki67/MIB1 Index, 1p19q codeletions, ATRX, EGFR, p53 expression, and O6-methylguanine DNA methyltransferase promotor methylation), were related to progression-free survival (PFS), malignant transformation-free survival (MTFS) and overall survival (OS)...
August 9, 2018: Neurosurgery
Felipe Pantoja Mesquita, Laine Celestino Pinto, Bruno Moreira Soares, Adrhyann Jullyanne de Sousa Portilho, Emerson Lucena da Silva, Ingryd Nayara de Farias Ramos, André Salim Khayat, Caroline Aquino Moreira-Nunes, Mirna Marques Bezerra, Eliza de Lucas Chazin, Thatyana Rocha Alves Vasconcelos, Rommel Mario Rodríguez Burbano, Maria Elisabete Amaral de Moraes, Raquel Carvalho Montenegro
Chemo-resistance has been reported as a relevant barrier for the efficiency of gastric cancer treatment. Therefore, the development of effective and safe drugs for cancer chemotherapy is still a challenge. The purpose of this study was to evaluate the anticancer potential of (E)-2-((2-(benzo[d]thiazo-2-yl)hydrazono)methyl)-4-nitrophenol) (AFN01) against gastric cancer cell lines. Our results showed promising anticancer activity against gastric cancer cells ACP-02 (IC50  = 1.0 μM) and mild activity against other cell lines including non-malignant gastric cell MNP-01 (IC50  = 3...
August 11, 2018: Chemico-biological Interactions
Ye Gu, Xinling Wang, Hong Liu, Guimei Li, Weiping Yu, Qing Ma
Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies worldwide. Histone‑lysine N‑methyltransferase SET7/9 is a protein lysine monomethylase that methylates histone H3K4 as well as various non‑histone proteins. Deregulation of SET7/9 is frequently detected in human cancers. However, the role of SET7/9 in HCC development remains unclear. In the present study, upregulation of SET7/9 and E2F transcription factor 1 (E2F1) expression was detected in 68 samples of HCC tissues compared with these levels noted in the paired healthy liver samples...
August 2, 2018: Oncology Reports
Ke Jin, Weixin Zhao, Xuan Xie, Yuejiang Pan, Kexi Wang, Huizhong Zhang
BACKGROUND: MicroRNAs (miRNAs) function as tumor promoting or tumor suppressing factors in many cancers. MiR-520b contributes to progression in head-neck and liver cancers, spinal osteosarcoma, and glioma; however, the association of miR-520b with lung cancer progression remains unknown. In this investigation, we explore the effect of miR-520b targeting HDAC4 on lung cancer growth. METHODS: The regulation of miR-520b or its inhibitor on HDAC4 expression was analyzed using Western blot analysis...
August 14, 2018: Thoracic Cancer
Yu Xiong, Wei Kuang, Shigang Lu, Hua Guo, Miaojing Wu, Minhua Ye, Lei Wu
Dysregulation of long noncoding RNAs (lncRNAs) has been implicated in human diseases, in particular, cancers. In this study, we determined the expression of an lncRNA, HOXB13-AS1, involving in glioma. We showed that HOXB13-AS1 was significantly upregulated in glioma tissues and cells and was negatively correlated with its surrounding gene HOXB13 levels. Functional experiments in vitro and in vivo revealed that high level of HOXB13-AS1 increased cell proliferation and tumor growth by promoting cell cycle progression...
August 13, 2018: Cancer Medicine
Alain Brice Tueche, Stephane Zingue, Edwige Nana Tchoupang, Telesphore Nanbo Gueyo, Abel Joël Gbaweng Yaya, Amstrong Nang Njuh, Dieudonnée Mireille Ntsa, Dieudonné Njamen
There is a long standing interest in the identification of medicinal plants and derived natural products for developing cancer therapeutics. The present study was designed to evaluate the in vitro and in vivo antiproliferative effects of Abyssinone V-4' methyl ether (AVME) on breast tissue of mice. The cytotoxicity of AVME was evaluated using MTT assay in four cancer cell lines (DU145, PC3, HepG2, and MCF-7). Further, a protective effect of AVME was evaluated on 7,12-dimethylbenz(a)anthracene- (DMBA-) induced breast tumor in Swiss mice...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Luciano Pirola, Oskar Ciesielski, Aneta Balcerczyk
Approximately 50 years ago, Judah Folkman raised the concept of inhibiting tumor angiogenesis for treating solid tumors. The development of anti-angiogenic drugs would decrease or even arrest tumor growth by restricting the delivery of oxygen and nutrient supplies, while at the same time display minimal toxic side effects to healthy tissues. Bevacizumab (Avastin)-a humanized monoclonal anti VEGF-A antibody-is now used as anti-angiogenic drug in several forms of cancers, yet with variable results. Recent years brought significant progresses in our understanding of the role of chromatin remodeling and epigenetic mechanisms in the regulation of angiogenesis and tumorigenesis...
August 10, 2018: Cancers
Jianping Lu, Juan Xu, Junyi Li, Tao Pan, Jing Bai, Liqiang Wang, Xiyun Jin, Xiaoyu Lin, Yunpeng Zhang, Yongsheng Li, Nidhi Sahni, Xia Li
Epigenetic alterations, a well-recognized cancer hallmark, are driven by chromatin regulators (CRs). However, little is known about the extent of CR deregulation in cancer, and less is known about their common and specialized roles across various cancers. Here, we performed genome-wide analyses and constructed molecular signatures and network profiles of functional CRs in over 10 000 tumors across 33 cancer types. By integration of DNA mutation, genome-wide methylation, transcriptional/post-transcriptional regulation, and protein interaction networks with clinical outcomes, we identified CRs associated with cancer subtypes and clinical prognosis as potential oncogenic drivers...
August 8, 2018: Nucleic Acids Research
Hjalte Sass, Per Cayé-Thomasen
In light of missing systematic reviews in the literature, the objective of this paper is to present the contemporary knowledge on the molecular biology of vestibular schwannomas (VS), based on a systematic literature search. In addition, current and prospected medical therapy based on molecular biology is addressed. A systematic literature search was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The systematic search was performed in the Pubmed and Embase databases...
August 3, 2018: Journal of International Advanced Otology
Suzana Dvojakovska, Danica Popovic-Monevska, Aleksandar Grcev, Goran Pancevski, Alberto Benedetti, Vladimir Popovski, Aleksandar Dimovski, Aleksandar Stamatoski
The advancements in epigenetics of oral squamous cell carcinoma (OSCC), are made in regard to DNA hypermethylation of MGMT, DAPK, ECAD (E-cadherin) and p16, as an important component of oral carcinogenesis and new potential biomarkers in molecular diagnostic strategies. The objective of the study was to evaluate the methylation status of the proposed genes and their possible role in the tumor genesis and diagnosis of OSCC. MATERIALS AND METHODS: From sixty surgically treated and molecularly analyzed patients, we obtained three groups of bioptical materials: tumor, normal contralateral and healthy tissues...
July 29, 2018: Journal of Cranio-maxillo-facial Surgery
Jean-Louis Pujol, Benoît Roch, Camille N Pujol, Catherine Goze
Small cell lung cancer accounts for 14% of all lung cancers. It remains a major challenge for oncology as the progresses made in the past three decades are modest. After a rapid overview of current knowledge regarding somatic genomic alterations, this state-of-art addresses pathways to improve small-cell lung cancer outcome such as the targeting of DNA damage repair mechanisms firstly anti-PARPs, inhibitory molecules of EZH2, derepression of the NOTCH pathway, rovalbituzumab-tesirine, inhibition of serine/threonine Aurora A kinase, temozolomide and its dependence on methylation of the MGMT promoter...
August 9, 2018: Bulletin du Cancer
Ana Carolina de Carvalho, Matias Eliseo Melendez, Cristina da Silva Sabato, Edenir Inez Palmero, Lidia Maria Rebolho Batista Arantes, Cristovam Scapulatempo Neto, André Lopes Carvalho
A better understanding of the clinical and molecular features of oropharyngeal squamous cell carcinomas (OPSCC) may help in the development of strategies for a better patient management, improving survival rates. This retrospective study conducted a clinical and molecular characterization of surgically treated OPSCC samples. Paraffin-embedded samples from a series of cases were screened for high-risk (HR) human papillomavirus (HPV) infection, methylation of a 5-gene panel, p53 expression, and TP53 mutation...
August 11, 2018: Pathology Oncology Research: POR
Meryem Gülfem Öner, Matjaz Rokavec, Markus Kaller, Nassim Bouznad, David Horst, Thomas Kirchner, Heiko Hermeking
BACKGROUND & AIMS: Combined inactivation of the microRNA 34a gene (MIR34A, by methylation) and the TP53 gene (by mutation or deletion) is observed in 50% of colorectal tumors that progress to distant metastases. We studied mice with intestinal disruption of Mir34a and Tp53 to investigate mechanisms of colorectal carcinogenesis and identify strategies to block these processes. METHODS: Mice with disruption of Mir34a and/or Tp53 specifically in intestinal epithelial cells (Mir34aΔIEC mice, Tp53ΔIEC mice, and Mir34aΔIEC /Tp53ΔIEC mice) and controls (Mir34aFl/Fl /Tp53Fl/Fl ) were given azoxymethane to induce colorectal carcinogenesis...
August 9, 2018: Gastroenterology
Chuanyong Lu, Daniel Ramirez, Sinchun Hwang, Achim Jungbluth, Denise Frosina, Peter Ntiamoah, John Healey, Guo Zhu, Wen Chen, Michael Klein, Meera Hameed
BACKGROUND: Histones are essential components of chromatin and mutations in histones lead to alterations in methylation and acetylation, which play an important role in tumorigenesis. Most of the chondroblastomas harbor H3K36M mutation. With the availability of mutation specific antibody, we aimed to assess the sensitivity of this antibody and the alterations of histone methylation in a series of chondroblastoma cases. DESIGN: Immunohistochemical stains using antibodies for H3K36M, trimethylated histones (H3K27me3 and H3K36me3), and osteoblastic marker (SATB2) were performed on 27 chondroblastomas from 27 patients...
August 11, 2018: Histopathology
Graeme Eisenhofer, Aleksander Prejbisz, Mirko Peitzsch, Christina Pamporaki, Jimmy Masjkur, Natalie Rogowski-Lehmann, Katharina Langton, Elena Tsourdi, Mariola Pęczkowska, Stephanie Fliedner, Timo Deutschbein, Felix Megerle, Henri J L M Timmers, Richard Sinnott, Felix Beuschlein, Martin Fassnacht, Andrzej Januszewicz, Jacques W M Lenders
BACKGROUND: Measurements of plasma or urinary metanephrines are recommended for diagnosis of pheochromocytoma and paraganglioma (PPGL). What test offers optimal diagnostic accuracy for patients at high and low risk of disease, whether urinary free metanephrines offer advantages over deconjugated metanephrines, and what are the utilities of inclusion of methoxytyramine in panels all remain unclear. METHODS: A population of 2056 patients with suspected PPGLs underwent prospective screening for disease using mass spectrometric-based measurements of plasma free, urinary deconjugated, and urinary free metanephrines and methoxytyramine...
August 10, 2018: Clinical Chemistry
Jianzhong Su, Yung-Hsin Huang, Xiaodong Cui, Xinyu Wang, Xiaotian Zhang, Yong Lei, Jianfeng Xu, Xueqiu Lin, Kaifu Chen, Jie Lv, Margaret A Goodell, Wei Li
BACKGROUND: Cancers have long been recognized to be not only genetically but also epigenetically distinct from their tissues of origin. Although genetic alterations underlying oncogene upregulation have been well studied, to what extent epigenetic mechanisms, such as DNA methylation, can also induce oncogene expression remains unknown. RESULTS: Here, through pan-cancer analysis of 4174 genome-wide profiles, including whole-genome bisulfite sequencing data from 30 normal tissues and 35 solid tumors, we discover a strong correlation between gene-body hypermethylation of DNA methylation canyons, defined as broad under-methylated regions, and overexpression of approximately 43% of homeobox genes, many of which are also oncogenes...
August 10, 2018: Genome Biology
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