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Multiple methods research

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https://www.readbyqxmd.com/read/30117418/-comparison-of-different-single-cell-whole-genome-amplification-methods-and-malbac-applications-in-assisted-reproduction
#1
Ya Xin Yao, Yong Fu La, Ran Di, Qiu Yue Liu, Wen Ping Hu, Xiang Yu Wang, Ming Xing Chu
Single-cell whole genome amplification (WGA) is a new technology, which can amplify small amounts of DNA from single cell and obtain the high coverage whole genome DNA template for revealing cell heterogeneity. Single cell WGA methods mainly include primer extension preamplification PCR (PEP-PCR), degenerate oligonucleotide primed PCR (DOP-PCR), multiple displacement amplification (MDA), and multiple annealing and looping-based amplification cycles (MALBAC). In this review, we describe the principles and applications of different single cell genome wide amplification, and we evaluate and compare their amplification efficiency, including the coverage of genome, homogeneity, reproducibility, and detection power of single-nucleotide variants (SNV) and copy number variants (CNV)...
August 16, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/30116702/daily-minutes-of-unprotected-sun-exposure-muse-inventory-measure-description-and-comparisons-to-uvr-sensor-and-sun-protection-survey-data
#2
Tammy K Stump, Lisa G Aspinwall, Elizabeth L Gray, Shuai Xu, Nenita Maganti, Sancy A Leachman, Nabil Alshurafa, June K Robinson
One in five US adults will be diagnosed with skin cancer. As most skin cancers are attributable to sun exposure, this risk factor is an important target for research and intervention. Most sun exposure measures assess frequency of specific sun-protection behaviors, which does not account for the use of multiple, potentially overlapping sun-protection methods. In contrast, the Daily Minutes of Unprotected Sun Exposure (MUSE) Inventory assesses sun-protection behavior during self-reported activities, providing several useful metrics, including duration of unprotected sun exposure on 17 body sites, combined to yield an overall MUSE score weighted by percent of body exposed...
September 2018: Preventive Medicine Reports
https://www.readbyqxmd.com/read/30116651/pharmacokinetics-and-bioavailability-study-of-tubeimoside-i-in-icr-mice-by-uplc-ms-ms
#3
Lianguo Chen, Qinghua Weng, Feifei Li, Jinlai Liu, Xueliang Zhang, Yunfang Zhou
The aim of this study is to establish and validate a rapid, selective, and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine tubeimoside I (TBMS-I) in ICR (Institute of Cancer Research) mouse whole blood and its application in the pharmacokinetics and bioavailability study. The blood samples were precipitated by acetonitrile to extract the analytes. Chromatographic separation was performed on a UPLC BEH C18 column (2.1 mm × 50 mm, 1.7  μ m)...
2018: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/30116096/anti-racism-methods-for-big-data-research-lessons-learned-from-the-hiv-testing-linkage-retention-in-care-hiv-tlr-study
#4
Chandra L Ford, Lois M Takahashi, P Paul Chandanabhumma, Maria Elena Ruiz, William E Cunningham
Public Health Critical Race Praxis (PHCRP) contributes three functional elements to health equity studies: a race conscious orientation; an antiracism lexicon based on Critical Race Theory (CRT); and an integrated, reflexive approach. Few big data studies employ all three functional elements. Therefore, this article describes the application of PHCRP to the Human Immunodeficiency Virus Testing, Linkage and Retention in care (HIV TLR) study (N=3,476,741), which connects multiple large datasets to electronic medical records to examine contextual determinants of racial/ethnic disparities in HIV care continuum outcomes in southern California...
2018: Ethnicity & Disease
https://www.readbyqxmd.com/read/30115811/remote-ischemic-conditioning-a-promising-therapeutic-intervention-for-multi-organ-protection
#5
Da Zhou, Jiayue Ding, Jingyuan Ya, Liqun Pan, Yuan Wang, Xunming Ji, Ran Meng
Despite decades of formidable exploration, multi-organ ischemia-reperfusion injury (IRI) encountered, particularly amongst elderly patients with clinical scenarios, such as age-related arteriosclerotic vascular disease, heart surgery and organ transplantation, is still an unsettled conundrum that besets clinicians. Remote ischemic conditioning (RIC), delivered via transient, repetitive noninvasive IR interventions to distant organs or tissues, is regarded as an innovative approach against IRI. Based on the available evidence, RIC holds the potential of affording protection to multiple organs or tissues, which include not only the heart and brain, but also others that are likely susceptible to IRI, such as the kidney, lung, liver and skin...
August 16, 2018: Aging
https://www.readbyqxmd.com/read/30115030/construction-of-high-density-linkage-maps-for-mapping-quantitative-trait-loci-for-multiple-traits-in-field-pea-pisum-sativum-l
#6
Krishna K Gali, Yong Liu, Anoop Sindhu, Marwan Diapari, Arun S K Shunmugam, Gene Arganosa, Ketema Daba, Carolyn Caron, Reddy V B Lachagari, Bunyamin Tar'an, Thomas D Warkentin
BACKGROUND: The objective of this research was to map quantitative trait loci (QTLs) of multiple traits of breeding importance in pea (Pisum sativum L.). Three recombinant inbred line (RIL) populations, PR-02 (Orb x CDC Striker), PR-07 (Carerra x CDC Striker) and PR-15 (1-2347-144 x CDC Meadow) were phenotyped for agronomic and seed quality traits under field conditions over multiple environments in Saskatchewan, Canada. The mapping populations were genotyped using genotyping-by-sequencing (GBS) method for simultaneous single nucleotide polymorphism (SNP) discovery and construction of high-density linkage maps...
August 16, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/30115026/simultaneous-detection-of-lung-fusions-using-a-multiplex-rt-pcr-next-generation-sequencing-based-approach-a-multi-institutional-research-study
#7
Cecily P Vaughn, José Luis Costa, Harriet E Feilotter, Rosella Petraroli, Varun Bagai, Anna Maria Rachiglio, Federica Zito Marino, Bastiaan Tops, Henriette M Kurth, Kazuko Sakai, Andrea Mafficini, Roy R L Bastien, Anne Reiman, Delphine Le Corre, Alexander Boag, Susan Crocker, Michel Bihl, Astrid Hirschmann, Aldo Scarpa, José Carlos Machado, Hélène Blons, Orla Sheils, Kelli Bramlett, Marjolijn J L Ligtenberg, Ian A Cree, Nicola Normanno, Kazuto Nishio, Pierre Laurent-Puig
BACKGROUND: Gene fusion events resulting from chromosomal rearrangements play an important role in initiation of lung adenocarcinoma. The recent association of four oncogenic driver genes, ALK, ROS1, RET, and NTRK1, as lung tumor predictive biomarkers has increased the need for development of up-to-date technologies for detection of these biomarkers in limited amounts of material. METHODS: We describe here a multi-institutional study using the Ion AmpliSeq™ RNA Fusion Lung Cancer Research Panel to interrogate previously characterized lung tumor samples...
August 16, 2018: BMC Cancer
https://www.readbyqxmd.com/read/30114381/sereega-simulating-event-related-eeg-activity
#8
Laurens R Krol, Juliane Pawlitzki, Fabien Lotte, Klaus Gramann, Thorsten O Zander
BACKGROUND: Electroencephalography (EEG) is a popular method to monitor brain activity, but it is difficult to evaluate EEG-based analysis methods because no ground-truth brain activity is available for comparison. Therefore, in order to test and evaluate such methods, researchers often use simulated EEG data instead of actual EEG recordings. Simulated data can be used, among other things, to assess or compare signal processing and machine learning algorithms, to model EEG variabilities, and to design source reconstruction methods...
August 13, 2018: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/30113859/comparative-effectiveness-study-in-multiple-sclerosis-patients-using-instrumental-variable-analysis
#9
Hamed Hosseini, Mohammad Ali Mansournia, Seyed Massood Nabavi, Ali Asghar Akhlaghi, Jaleh Gholami, Kazem Mohammad, Reza Majdzadeh
Randomized clinical trials are considered the ideal source for generation of robust evidence for clinical and public health decision making. Estimation of treatment effect in observational studies is always subject to varying degrees of bias due to lack of random allocation, blindness, precise definition of intervention, as well as the existence of potential unknown and unmeasured confounding variables. Unlike other conventional methods, instrumental variable analysis (IVA), as a method for controlling confounding bias in non-randomized studies, attempts to estimate the treatment effect with the least bias even without knowing and measuring the potential confounders in the causal pathway...
August 1, 2018: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/30113753/modelling-white-matter-with-spherical-deconvolution-how-and-why
#10
Flavio Dell'Acqua, J-Donald Tournier
Since the realization that diffusion MRI can probe the microstructural organization and orientation of biological tissue in vivo and non-invasively, a multitude of diffusion imaging methods have been developed and applied to study the living human brain. Diffusion tensor imaging was the first model to be widely adopted in clinical and neuroscience research, but it was also clear from the beginning that it suffered from limitations when mapping complex configurations, such as crossing fibres. In this review, we highlight the main steps that have led the field of diffusion imaging to move from the tensor model to the adoption of diffusion and fibre orientation density functions as a more effective way to describe the complexity of white matter organization within each brain voxel...
August 16, 2018: NMR in Biomedicine
https://www.readbyqxmd.com/read/30111531/virtual-medical-modality-implementation-strategies-for-patient-aligned-care-teams-to-promote-veteran-centered-care-protocol-for-a-mixed-methods-study
#11
Jolie Haun, Margeaux Chavez, Wendy Hathaway, Nicole Antinori, Christine Melillo, Bridget A Cotner, Julie McMahon-Grenz, Brian Zilka, Shilpa Patel-Teague, William Messina, Kim Nazi
BACKGROUND: The Veterans Health Administration (VHA) is making system-wide efforts to increase integrated use of health information technology (HIT), including My HealtheVet (MHV), the Veterans Affairs (VA) electronic patient portal, Vet Link kiosks, telehealth, and mobile apps. Integrated use of HIT can increase individual and system efficiency, maximize resources, and enhance patient outcomes. Prior research indicates that provider endorsement and reinforcement are key determinants of patient adoption of HIT...
August 15, 2018: JMIR Research Protocols
https://www.readbyqxmd.com/read/30111048/-morphological-microscopic-multiple-component-assay-and-fingerprinting-based-systematic-research-on-quality-evaluation-of-moutan-cortex-paeonia-suffruticosa
#12
Zhi-Qiang Wang, Yong Chen, Shuang-Shuang Liu, Hong-Xiang Li, Wei-Wen Li, Ling Dong, Chun-Nian He, Fei-Hu Chen, Pei-Gen Xiao
The purpose of this study was to combine morphological, microscopic, UHPLC multiple-component assay and fingerprinting studies in order to evaluate the quality of Moutan Cortex (MC) systematically. The root system of Paeonia suffruticosa was measured to compare the morphological variation and the chemical composition of different grades of MC was discussed according to previous studies. The difference between the main microscopic features of MC powder and the xylem powder is dramatic, the MC powder contains great amount of starch granules and clusters of calcium oxalate, while the xylem powder displays considerable vessels...
July 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/30109417/toxoplasma-gondii-infection-and-behavioral-outcomes-in-humans-a-systematic-review
#13
REVIEW
Victor Otero Martinez, Fernanda Washington de Mendonça Lima, Chrissie Ferreira de Carvalho, José Antônio Menezes-Filho
Studies suggest that the protozoan Toxoplasma gondii can disturb human behavior. This study aimed to systematically review the scientific literature on the possible associations between Toxoplasma gondii infection and neurobehavioral abnormalities in humans. We reviewed and summarized the studies published since 1990. The descriptors used were related to T. gondii infection and behavioral outcomes in humans; the main databases of the medical literature were accessed. The results of eight original articles published between 1994 and 2016 were evaluated and described...
August 14, 2018: Parasitology Research
https://www.readbyqxmd.com/read/30107788/defining-the-process-to-literature-searching-in-systematic-reviews-a-literature-review-of-guidance-and-supporting-studies
#14
REVIEW
Chris Cooper, Andrew Booth, Jo Varley-Campbell, Nicky Britten, Ruth Garside
BACKGROUND: Systematic literature searching is recognised as a critical component of the systematic review process. It involves a systematic search for studies and aims for a transparent report of study identification, leaving readers clear about what was done to identify studies, and how the findings of the review are situated in the relevant evidence. Information specialists and review teams appear to work from a shared and tacit model of the literature search process. How this tacit model has developed and evolved is unclear, and it has not been explicitly examined before...
August 14, 2018: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/30107438/does-the-dospert-scale-predict-risk-taking-behaviour-during-travel-a-study-using-smartphones
#15
Andrea Farnham, Sarah Ziegler, Ulf Blanke, Emily Stone, Christoph Hatz, Milo A Puhan
Background: Despite the continuing growth of international tourism, very little research has been done on the link between individual risk attitudes and health behaviours during travel. Our study uses a validated risk-taking questionnaire (DOSPERT) and data from a smartphone application to study the association between pre-travel risk attitudes and the occurrence of behaviours during travel. Methods: A prospective cohort of travellers to Thailand used a smartphone application to answer a daily questionnaire about health behaviours and events...
August 10, 2018: Journal of Travel Medicine
https://www.readbyqxmd.com/read/30107199/extracellular-biosynthesis-of-biocompatible-cadmium-sulfide-quantum-dots-using-trametes-versicolor
#16
Zhijie Qin, Qiulin Yue, Yan Liang, Jingjing Zhang, Lin Zhou, Orlando Borrás Hidalgo, Xinli Liu
Quantum dots are important fluorescent semiconductor nano-crystals with distinguished electrical and optical properties and have gained great interest in many fields. The chemical and physical synthetic methods are usually not favorable for biological application due to high energy-consumption procedure and residual toxic chemicals. The development of novel "green" routes to prepare bio-compatible cadmium sulfide quantum dots constitutes a promising substituted approach. We used the white rot fungus Trametes versicolor for the biosynthesis of cadmium sulfide quantum dots taking account of the adsorption property of this fungus...
August 11, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/30104743/pmlpr-a-novel-method-for-predicting-subcellular-localization-based-on-recommender-systems
#17
Elnaz Mirzaei Mehrabad, Reza Hassanzadeh, Changiz Eslahchi
The importance of protein subcellular localization problem is due to the importance of protein's functions in different cell parts. Moreover, prediction of subcellular locations helps to identify the potential molecular targets for drugs and has an important role in genome annotation. Most of the existing prediction methods assign only one location for each protein. But, since some proteins move between different subcellular locations, they can have multiple locations. In recent years, some multiple location predictors have been introduced...
August 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30103695/the-swiss-multiple-sclerosis-registry-smsr-study-protocol-of-a-participatory-nationwide-registry-to-promote-epidemiological-and-patient-centered-ms-research
#18
Nina Steinemann, Jens Kuhle, Pasquale Calabrese, Jürg Kesselring, Giulio Disanto, Doron Merkler, Caroline Pot, Vladeta Ajdacic-Gross, Stephanie Rodgers, Milo Alan Puhan, Viktor von Wyl
BACKGROUND: Multiple sclerosis (MS) is one of the most frequently observed neurological conditions in Switzerland, but data sources for country-wide epidemiological trend monitoring are lacking. Moreover, while clinical and laboratory MS research are generally well established, there is a gap in patient-centered MS research to inform care management, or treatment decisions and policy making not only in Switzerland but worldwide. METHODS: In light of these research gaps, the Swiss Multiple Sclerosis Society initiated and funded the Swiss Multiple Sclerosis Registry (SMSR) an open-ended, longitudinal and prospective, nationwide, patient-centered study...
August 13, 2018: BMC Neurology
https://www.readbyqxmd.com/read/30103153/qualitative-findings-from-an-australian-study-of-inter-partner-violence-content-within-undergraduate-health-curriculum
#19
Renee Lovi, John Hurley, Marie Hutchinson
Health care professionals are frequently one of the first points of contact in episodes of intimate partner violence (IPV). This paper presents qualitative data from a larger research project on IPV-related content in undergraduate curriculum for Australian health care professionals. IPV is a serious public health issue with findings from this paper supporting the proposition that key health care workers such as nurses, midwives and paramedics are ill prepared to respond to the challenges it poses. OBJECTIVE: This study sought to better understand the barriers and facilitators to the inclusion of IPV-related content into undergraduate curricula for nurses, midwives and paramedics...
August 1, 2018: Nurse Education Today
https://www.readbyqxmd.com/read/30102286/key-elements-of-photo-attraction-bioassay-for-insect-studies-or-monitoring-programs
#20
Lee W Cohnstaedt, Jordan C Disberger, Eric Paulsen, Adrian J Duehl
Optimized visual attractants will increase insect trapping efficiency by using the target insect's innate behaviors (positive photo-taxis) as a means to lure the insect into a population control or monitoring trap. Light emitting diodes (LEDs) have created customizable lighting options with specific wavelengths (colors), intensities, and bandwidths, all of which can be customized to the target insects. Photo-attraction behavioral bioassays can use LEDs to optimize the attractive color(s) for an insect species down to specific life history stages or behaviors (mating, feeding, or seeking shelter)...
July 26, 2018: Journal of Visualized Experiments: JoVE
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