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Sperm ZP receptors

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https://www.readbyqxmd.com/read/29751768/the-preclinical-evaluation-of-immunocontraceptive-vaccines-based-on-canine-zona-pellucida-3-czp3-in-a-mouse-model
#1
Ying Wang, Yijie Li, Beibei Zhang, Fuchun Zhang
BACKGROUND: Stray dogs are the reservoirs and carriers of rabies and are definitive hosts of echinococcosis. To control the overpopulation of stray dogs, zona pellucida 3 (ZP3), a primary receptor for sperm, is a potential antigen for developing contraceptive vaccines. To enhance the immune responses and contraceptive effects of canine ZP3 (cZP3), dog gonadotropin-releasing hormone (GnRH) and a T cell epitope of chicken ovalbumin (OVA) were selected to construct two fusion proteins with cZP3, ovalbumin-GnRH-ZP3 (OGZ) and ovalbumin-ZP3 (OZ), and their contraceptive effects were evaluated in mice...
May 11, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29708094/urea-changes-oocyte-competence-and-gene-expression-in-resultant-bovine-embryo-in-vitro
#2
Rasoul Kowsar, Fatemeh Izadi, Nima Sadeghi, Ahmad Riasi, Faezeh Ghazvini Zadegan, Mehdi Hajian, Mohammad Hossein Nasr-Esfahani, Hossein Farrokhpour, Akio Miyamoto
SummaryNutrition influences the microenvironment in the proximity of oocyte and affects early embryonic development. Elevated blood urea nitrogen, even in healthy dairy cows, is associated with reduced fertility and there is high correlation between blood urea levels and follicular fluid urea levels. Using a docking calculation (in silico), urea showed a favorable binding activity towards the ZP-N domain of ZP3, that of ZP2, and towards the predicted full-length sperm receptor ZP3. Supplementation of oocyte maturation medium with nutrition-related levels of urea (20 or 40 mg/dl as seen in healthy dairy cows fed on low or high dietary protein, respectively) dose-dependently increased: (i) the proportion of oocytes that remained uncleaved; and (ii) oocyte degeneration; and reduced cleavage, blastocyst and hatching rates...
June 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28460669/pig-sperm-preincubation-and-gamete-coincubation-with-glutamate-enhance-sperm-oocyte-binding-and-in-vitro-fertilization
#3
M Spinaci, D Bucci, B Gadani, E Porcu, C Tamanini, G Galeati
As the taste receptor for monosodium glutamate (umami) is expressed in both murine and human spermatozoa and the presence of α-gustducin and α-transducin, G proteins involved in the umami taste signaling, has been described in boar germ cells, the aim of this study was to evaluate if monosodium glutamate (MSG) would exert any effect on sperm-oocyte binding, in vitro fertilization (IVF) and sperm parameters during in vitro induced capacitation. For sperm-zona pellucida binding assay, boar spermatozoa were preincubated for 1 h and then coincubated for 1 h with denuded in vitro matured oocytes in presence of different concentrations of MSG (0, 0...
June 2017: Theriogenology
https://www.readbyqxmd.com/read/28175305/the-roles-of-protein-disulphide-isomerase-family-a-member-3-erp57-and-surface-thiol-disulphide-exchange-in-human-spermatozoa-zona-pellucida-binding
#4
Chi-Wai Wong, Kevin K W Lam, Cheuk-Lun Lee, William S B Yeung, Wei E Zhao, Pak-Chung Ho, Jian-Ping Ou, Philip C N Chiu
Study question: Are multimeric sperm plasma membrane protein complexes, ERp57 and sperm surface thiol content involved in human spermatozoa-zona pellucida (ZP) interaction? Summary answer: ERp57 is a component of a multimeric spermatozoa-ZP receptor complex involved in regulation of human spermatozoa-ZP binding via up-regulation of sperm surface thiol content. What is known already: A spermatozoon acquires its fertilization capacity within the female reproductive tract by capacitation...
April 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28145526/zp3-is-required-for-germinal-vesicle-breakdown-in-mouse-oocyte-meiosis
#5
Lei-Lei Gao, Chun-Xiang Zhou, Xiao-Lan Zhang, Peng Liu, Zhen Jin, Gang-Yi Zhu, Yang Ma, Jing Li, Zhi-Xia Yang, Dong Zhang
ZP3 is a principal component of the zona pellucida (ZP) of mammalian oocytes and is essential for normal fertility, and knockout of ZP3 causes complete infertility. ZP3 promotes fertilization by recognizing sperm binding and activating the acrosome reaction; however, additional cellular roles for ZP3 in mammalian oocytes have not been yet reported. In the current study, we found that ZP3 was strongly expressed in the nucleus during prophase and gradually translocated to the ZP. Knockdown of ZP3 by a specific siRNA dramatically inhibited germinal vesicle breakdown (GVBD) (marking the beginning of meiosis), significantly reducing the percentage of MII oocytes...
February 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/26935599/plasminogen-improves-mouse-ivf-by-interactions-with-inner-acrosomal-membrane-bound-mmp2-and-samp14
#6
Marvin J S Ferrer, Wei Xu, Jagathpala Shetty, John Herr, Richard Oko
Spermatozoa must penetrate the outer investments of the oocyte, the cumulus oophorus and the zona pellucida (ZP), in order for fertilization to occur. This may require exposure of enzymes on the sperm's inner acrosomal membrane (IAM), one of which is matrix metalloproteinase (MMP) 2, to factors in oviductal fluid. Plasminogen is present in oviductal fluid and activates MMP2 in somatic tissues. The objectives of this study were: 1) to examine possible interactions between plasminogen and IAM-bound plasminogen activator receptor (SAMP14) and -MMP2, 2) to demonstrate plasminogen's presence in the extracellular environment at the site of fertilization, and 3) to provide evidence that plasminogen plays a role in fertilization...
April 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/26811476/a-structured-interdomain-linker-directs-self-polymerization-of-human-uromodulin
#7
Marcel Bokhove, Kaoru Nishimura, Martina Brunati, Ling Han, Daniele de Sanctis, Luca Rampoldi, Luca Jovine
Uromodulin (UMOD)/Tamm-Horsfall protein, the most abundant human urinary protein, plays a key role in chronic kidney diseases and is a promising therapeutic target for hypertension. Via its bipartite zona pellucida module (ZP-N/ZP-C), UMOD forms extracellular filaments that regulate kidney electrolyte balance and innate immunity, as well as protect against renal stones. Moreover, salt-dependent aggregation of UMOD filaments in the urine generates a soluble molecular net that captures uropathogenic bacteria and facilitates their clearance...
February 9, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26610590/the-hinge-region-of-bovine-zona-pellucida-glycoprotein-zp3-is-involved-in-the-formation-of-the-sperm-binding-active-zp3-zp4-complex
#8
Kaori Suzuki, Nanami Tatebe, Sayuri Kojima, Ayumi Hamano, Misaki Orita, Naoto Yonezawa
The zona pellucida (ZP) surrounds the mammalian oocyte and mediates species-selective sperm-oocyte interactions. Bovine ZP consists of glycoproteins ZP2, ZP3, and ZP4. Neither ZP3 nor ZP4 alone shows inhibitory activity for the binding of sperm to the ZP; however, this activity is seen with the ZP3/ZP4 heterocomplex. Here, we constructed a series of bovine ZP3 mutants to identify the ZP4-binding site on ZP3. Each ZP3 mutant was co-expressed with ZP4 using a baculovirus-Sf9 cell expression system and examined for interaction with ZP4 as well as inhibitory activity for sperm-ZP binding...
November 23, 2015: Biomolecules
https://www.readbyqxmd.com/read/26329136/human-sperm-degradation-of-zona-pellucida-proteins-contributes-to-fertilization
#9
Analilia Saldívar-Hernández, María E González-González, Ana Sánchez-Tusié, Israel Maldonado-Rosas, Pablo López, Claudia L Treviño, Fernando Larrea, Mayel Chirinos
BACKGROUND: The mammalian oocyte extracellular matrix known as the zona pellucida (ZP) acts as a barrier to accomplish sperm fusion with the female gamete. Although penetration of the ZP is a limiting event to achieve fertilization, this is one of the least comprehended stages of gamete interaction. Even though previous studies suggest that proteases of sperm origin contribute to facilitate the passage of sperm through the ZP, in human this process is not yet fully understood. The aim of this study was to determine the ability of human sperm to degrade recombinant human ZP (rhZPs) proteins and to characterize the proteases involved in this process...
September 2, 2015: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/26154327/in-silico-identification-of-the-genes-for-sperm-egg-interaction-in-the-internal-fertilization-of-the-newt-cynops-pyrrhogaster
#10
Akihiko Watanabe, Eriko Takayama-Watanabe
A specific sperm-egg interaction in the oviductal matrix is crucial for internal fertilization of the red-bellied newt, Cynops pyrrhogaster. An understanding of the molecular basis of this interaction is expected to elucidate the evolutionary history of internal fertilization in amphibians. Recently, deep sequencing technology has provided global gene information even in nonmodel animals, allowing us to understand specific features of the molecular mechanisms underlying fertilization in C. pyrrhogaster. In the present study, we screened de novo assembled RNAseq from ovary, testis, and oviduct samples in C...
2014: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/25623231/four-glycoproteins-are-expressed-in-the-cat-zona-pellucida
#11
I Stetson, M Avilés, C Moros, F A García-Vázquez, L Gimeno, A Torrecillas, C Aliaga, M V Bernardo-Pisa, J Ballesta, M J Izquierdo-Rico
The mammalian oocyte is surrounded by a matrix called the zona pellucida (ZP). This envelope participates in processes such as acrosome reaction induction, sperm binding and may be involved in speciation. In cat (Felis catus), this matrix is composed of at least three glycoproteins called ZP2, ZP3, and ZP4. However, recent studies have pointed to the presence of a fourth protein in several mammals (rat, human, hamster or rabbit), meaning that a reevaluation of cat ZP is needed. For this reason, the objective of this research was to analyze the protein composition of cat ZP by means of proteomic analysis...
April 15, 2015: Theriogenology
https://www.readbyqxmd.com/read/25529998/panel-of-monoclonal-antibodies-to-sperm-surface-proteins-as-a-tool-for-monitoring-localization-and-identification-of-sperm-zona-pellucida-receptors
#12
Michal Zigo, Andriy Dorosh, Alžběta Pohlová, Věra Jonáková, Miroslav Šulc, Pavla Maňásková-Postlerová
Primary binding of the sperm to the zona pellucida (ZP) is one of the many steps necessary for successful fertilization. Sperm bind ZP by means of membrane receptors which recognize carbohydrate moieties on ZP glycoproteins according to a well-defined sequential process. Primary binding receptors, many of which have been disclosed in various mammals, are localized throughout the acrosomal region of the sperm surface. A panel of monoclonal antibodies against proteins from the sperm surface was prepared. Antibodies were screened by immunofluorescence for protein localization and Western blotting...
March 2015: Cell and Tissue Research
https://www.readbyqxmd.com/read/25445843/role-of-zona-pellucida-glycoproteins-during-fertilization-in-humans
#13
REVIEW
Satish Kumar Gupta
In the last decade, scientific investigations pertaining to the role of zona pellucida (ZP) glycoproteins during fertilization in humans have led to new insights. This has been achieved using purified native/recombinant human zona proteins and transgenic mice expressing human ZP glycoproteins. The proposed model in mice of ZP glycoprotein-3 (ZP3) acting as primary sperm receptor and ZP glycoprotein-2 (ZP2) as secondary sperm receptor has been modified for sperm-egg binding in humans. ZP glycoprotein-1 (ZP1), ZP3, and ZP glycoprotein-4 (ZP4) have been shown to bind to the capacitated human sperm...
April 2015: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/25253730/glycosylated-chicken-zp2-accumulates-in-the-egg-coat-of-immature-oocytes-and-remains-localized-to-the-germinal-disc-region-of-mature-eggs
#14
Shunsuke Nishio, Yoshinori Kohno, Yuki Iwata, Mayumi Arai, Hiroki Okumura, Kenzi Oshima, Daita Nadano, Tsukasa Matsuda
Vertebrate eggs are surrounded by an egg coat, which is a specific extracellular egg matrix consisting of several glycoproteins with a conserved zona pellucida (ZP) domain. Two mammalian egg coat subunits, ZP2 and ZP3, have been suggested to act as sperm receptors. In bird eggs, however, ZP2 has never been identified in the egg coat of mature oocytes and ovulated eggs. Here we report that chicken ZP2 is expressed in immature small follicles and remains as an egg-coat component locally in the germinal disc region of mature eggs...
November 2014: Biology of Reproduction
https://www.readbyqxmd.com/read/25168311/an-in-vitro-study-of-the-effect-of-mifepristone-and-ulipristal-acetate-on-human-sperm-functions
#15
J K Y Ko, V W Huang, R H W Li, W S B Yeung, P C Ho, P C N Chiu
Ulipristal acetate (UPA) and mifepristone are currently well-established agents for emergency contraception. Both drugs are selective progestogen receptor modulators which have been shown to have better efficacy than the widely used levonorgestrel in prevention of pregnancy. However, there is only limited information on the action of UPA on sperm function. The present study compared the in vitro biological effects of mifepristone and UPA on human sperm functions. Spermatozoa from semen samples with normal semen parameters were isolated...
November 2014: Andrology
https://www.readbyqxmd.com/read/25078272/proteomic-characterization-of-pig-sperm-anterior-head-plasma-membrane-reveals-roles-of-acrosomal-proteins-in-zp3-binding
#16
Kessiri Kongmanas, Hathairat Kruevaisayawan, Arpornrad Saewu, Clarissa Sugeng, Jason Fernandes, Puneet Souda, Jonathan B Angel, Kym F Faull, R John Aitken, Julian Whitelegge, Daniel Hardy, Trish Berger, Mark A Baker, Nongnuj Tanphaichitr
The sperm anterior head plasma membrane (APM) is the site where sperm first bind to the zona pellucida (ZP). This binding reaches the maximum following the sperm capacitation process. To gain a better understanding of the sperm-ZP binding mechanisms, we compared protein profiles obtained from mass spectrometry of APM vesicles isolated from non-capacitated and capacitated sperm. The results revealed that ZP-binding proteins were the most abundant group of proteins, with a number of them showing increased levels in capacitated sperm...
February 2015: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/25054321/molecular-and-cellular-mechanisms-of-sperm-oocyte-interactions-opinions-relative-to-in-vitro-fertilization-ivf
#17
REVIEW
George Anifandis, Christina Messini, Konstantinos Dafopoulos, Sotiris Sotiriou, Ioannis Messinis
One of the biggest prerequisites for pregnancy is the fertilization step, where a human haploid spermatozoon interacts and penetrates one haploid oocyte in order to produce the diploid zygote. Although fertilization is defined by the presence of two pronuclei and the extraction of the second polar body the process itself requires preparation of both gametes for fertilization to take place at a specific time. These preparations include a number of consecutive biochemical and molecular events with the help of specific molecules and with the consequential interaction between the two gametes...
July 22, 2014: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/25030762/posttranslational-modifications-of-zona-pellucida-proteins
#18
REVIEW
Naoto Yonezawa
The zona pellucida (ZP), which surrounds the mammalian oocyte, functions in various aspects of fertilization. The ZP consists of three or four glycoproteins, which are derived from transmembrane proteins that lack the ability to self-assemble. Following posttranslational processing at specific sites, ectodomains of ZP precursor proteins are released from the membrane and begin to form a matrix. Glycosylational modification is thought to be involved in species-selective sperm recognition by ZP proteins. However, in mice, the supramolecular structure of the zona matrix is also important in sperm recognition...
2014: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/25023718/erk1-2-mediates-sperm-acrosome-reaction-through-elevation-of-intracellular-calcium-concentration
#19
Yael Jaldety, Haim Breitbart
Mammalian sperm acquire fertilization capacity after residing in the female reproductive tract for a few hours in a process called capacitation. Only capacitated sperm can bind the zona pellucida (ZP) of the egg and undergo the acrosome reaction, a process that allows penetration and fertilization. Extracellular signal regulated kinase (ERK1/2) mediates signalling in many cell types, however its role in sperm function is largely unknown. Here we show that ERK1/2 is highly phosphorylated/activated after a short incubation of mouse sperm under capacitation conditions and that this phosphorylation is reduced after longer incubation...
October 2015: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/25020059/significance-of-egg-s-zona-pellucida-glycoproteins-in-sperm-egg-interaction-and-fertilization
#20
REVIEW
A Abou-Haila, M Bendahmane, D R Tulsiani
Mammalian fertilization is a highly programmed process by which sperm and egg unite to form a zygote, a cell with somatic chromosome numbers. To fertilize an egg, the capacitated (acrosome-intact) spermatozoa recognize and bind to the egg's extracellular glycocalyx coat, the zona pellucida (ZP). The tight and irreversible binding of the opposite gametes in the mouse and many other species studied, including man, results in the opening of Ca2+ channels on sperm plasma membrane (PM) and influx of Ca2+. The transient rise in Ca2+ and other second messengers, such as cAMP and IP3, initiates a cascade of signaling events that elevate sperm pH and triggers the fusion of the sperm PM and underlying outer acrosomal membrane at multiple sites (induction of the acrosomal reaction)...
August 2014: Minerva Ginecologica
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