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Sperm acrosome reaction

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https://www.readbyqxmd.com/read/30328351/inhibition-of-tmem16a-impedes-embryo-implantation-and-decidualization-in-mice
#1
Qianrong Qi, Yifan Yang, Kailin Wu, Qingzhen Xie
Recent studies revealed that TMEM16A is involved in several reproductive processes, including ovarian estrogen secretion and ovulation, sperm motility and acrosome reaction, fertilization and myometrium contraction. However, little is known about the expression and function of TMEM16A in embryo implantation and decidualization. In this study, we focused on the expression and regulation of TMEM16A in mouse uterus during early pregnancy. We found that TMEM16A is upregulated in uterine endometrium in response to embryo implantation and decidualization...
October 2, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/30328350/deletion-of-eqtn-in-mice-reduces-male-fertility-and-sperm%C3%A2-egg-adhesion
#2
Chizuru Ito, Kenji Yamatoya, Keiichi Yoshida, Lisa Fujimura, Hajime Sugiyama, Akiko Suganami, Yutaka Tamura, Masahiko Hatano, Kenji Miyado, Kiyotaka Toshimori
A number of sperm proteins are involved in the processes from gamete adhesion to fusion, but the underlying mechanism is still unclear. Here, we established a mouse mutant, the EQUATORIN-knockout (EQTN-KO, Eqtn−/−) mouse model and found that the EQTN-KO males have reduced fertility and sperm–egg adhesion, while the EQTN-KO females are fertile. Eqtn−/− sperm were normal in morphology and motility. Eqtn−/−-Tg (Acr-Egfp) sperm, which were produced as the acrosome reporter by crossing Eqtn−/− with Eqtn+/+-Tg(Acr-Egfp) mice, traveled to the oviduct ampulla and penetrated the egg zona pellucida of WT females...
October 1, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/30328343/proteomic-changes-in-uterine-flushings-after-levonorgestrel-treatment-and-effects-on-sperm-function
#3
Gabriela Hernández-Silva, Marta Durand, Fernando Larrea, Mayel Chirinos
When levonorgestrel (LNG) is given for emergency contraception during the follicular phase, it not only inhibits or delays ovulation, but also induces changes in endometrial secretions that modulate sperm functionality. In order to characterize the female reproductive tract secreted molecules that may affect human spermatozoa, we analyzed changes in the protein content of uterine flushings obtained from women during the periovulatory phase of a control and a LNG-treated menstrual cycle. Lectin affinity analysis and 2D gel electrophoresis of uterine samples showed changes in protein glycosylation patterns and the presence of 31 differentially expressed proteins (8 upregulated and 23 downregulated)...
September 12, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/30304664/tissue-plasminogen-activator-tpa-of-paternal-origin-is-necessary-for-the-success-of-invitro-but-not-of-invivo-fertilisation-in-the-mouse
#4
Francisco A García-Vázquez, C Soriano-Úbeda, R Laguna-Barraza, M José Izquierdo-Rico, Felipe A Navarrete, Pablo E Visconti, A Gutiérrez-Adán, P Coy
Besides its fibrinolytic function, the plasminogen-plasmin (PLG-PLA) system is also involved in fertilisation, where plasminogen activators bind to plasminogen to produce plasmin, which modulates sperm binding to the zona pellucida. However, controversy exists, depending on the species, concerning the role of the different components of the system. This study focused its attention on the role of the PLG-PLA system on fertilisation in the mouse with special attention to tissue plasminogen activator (tPA). The presence of exogenous plasminogen reduced in vitro fertilisation (IVF) rates and this decline was attenuated by the presence of plasmin inhibitors in combination with plasminogen...
October 10, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/30291684/possible-mechanisms-for-the-effects-of-calcium-deficiency-on-male-infertility
#5
Asghar Beigi Harchegani, Ali Irandoost, Mahdiyeh Mirnamniha, Hamid Rahmani, Eisa Tahmasbpour, Alireza Shahriary
Calcium (Ca) is a significant element that acts as an intracellular second messenger. It is necessary for many physiological processes in spermatozoa including spermatogenesis, sperm motility, capacitation, acrosome reaction and fertilization. Although influences of Ca deficiency on sperm function and male infertility have been widely studied, mechanisms for these abnormalities are not well considered. Poor sperm motility, impairment of chemotaxis, capacitation, acrosome reaction and steroidogenesis are the major mechanisms by which Ca deficiency induces male infertility...
January 2019: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/30268736/seasonal-variations-in-sperm-acrosome-reaction-osmotic-tolerance-and-serum-testosterone-concentrations-in-rams
#6
Ejaz Ahmad, Zahid Naseer, Uğur Uçan, İlker Serin, Ahmet Ceylan, Melih Aksoy
The study was conducted to evaluate the seasonal dynamics in the sperm acrosome reaction (AR), osmotic tolerance and serum testosterone concentration in rams. Blood and semen samples were collected from six mature rams during the winter, spring, summer and autumn seasons. The AR in sperm samples was induced by using lysophosphatidylcholine (LPC) and calcium ionophore (CI). The AR was monitored at various incubation times (15, 30, 60, 120 and 180 min) post-induction. To determine the osmotic tolerance through hypo-osmotic swelling test (HOST) the sperm were exposed to different osmotic conditions (50, 100, 290, 500 and 1000 mOsm/L)...
September 20, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30243139/dietary-quercetin-maintains-the-semen-quality-in-rabbits-under-summer-heat-stress
#7
Zahid Naseer, Ejaz Ahmad, Hande Sultan Şahiner, Erkmen Tuğrul Epikmen, Muhammad Fiaz, Muhamad Rizwan Yousuf, Shahzad Akbar Khan, İlker Serin, Ahmet Ceylan, Melih Aksoy
This study focused to determine beneficial impact of feeding quercetin supplemented diet on semen quality in summer heat imposed rabbits. Twelve heat stressed (HS) adult rabbits bucks were either fed with basal diet (HS; n = 06) or quercetin supplemented diet (QU-HS; n = 06) for a period of 56 days. Semen samples were collected and evaluated for volume, osmolality, morphology, concentration, motility, motion kinetics, viability, acrosome integrity, mitochondrial potential, and seminal plasma MDA level...
September 15, 2018: Theriogenology
https://www.readbyqxmd.com/read/30225889/paraoxonase-1-pon1-attenuates-sperm-hyperactivity-and-spontaneous-acrosome-reaction
#8
M Efrat, A Stein, H Pinkas, H Breitbart, R Unger, R Birk
BACKGROUND: Sperm capacitation is essential for proper fertilization and is associated with increased sperm hyperactivity (HA) and acrosome reaction (AR). For successful fertilization, AR timing is critical; accordingly, early spontaneous AR may not facilitate fertilization. Paraoxonase 1 (PON1) possesses antioxidant properties which affect sperm capacitation. The association between PON1, semen parameters, and capacitation is not fully understood. OBJECTIVE: To study PON1 activity in relation to human sperm hyperactivity and AR...
September 17, 2018: Andrology
https://www.readbyqxmd.com/read/30223333/triphenyltin-exposition-induces-spermatic-parameter-alters-of-calomys-laucha-species
#9
Tiane Ferreira de Castro, Graciela Quintana Saalfeld, Antonio Sergio Varela, Francine Ferreira Padilha, Klebson Silva Santos, Diego Martins Pires, Jessica Ribeiro Pereira, Carine Dahl Corcini, Elton Pinto Colares
The present study aims to evaluate the influence of triphenyltin (TPT) exposure on reproductive physiology on Calomys laucha species, since this species inhabits regions susceptible to exposure to this contaminant. Animals exposed to the highest dose (10.0 mg/kg) presented signs of severe intoxication in only 7 days of exposure, demonstrating a higher sensitivity of this species to triphenyltin. The 10.0 mg TPT/kg dose was analyzed separately for short-term exposure and results suggest that exposure to this dose was severely detrimental to sperm activity...
November 2018: Chemosphere
https://www.readbyqxmd.com/read/30217679/ageing-reduces-the-reproductive-performance-of-male-white-shrimp-litopenaeus-vannamei-by-altering-sperm-intracellular-ca-2-concentrations-and-interfering-with-sperm-apoptosis
#10
Wei Shi, Shanjie Zha, Lihua Hu, Xianke Huang, Jiongming Zhang, Maocang Yan, Guangxu Liu
Although the effects of age on the reproductive performance of various invertebrates, including white shrimp Litopenaeus vannamei are increasingly well documented, the mechanisms manifesting these impacts remain poorly understood. To ascertain the mechanisms of age on reproductive performance, the sperm quality, intracellular contents of Ca2+ , insemination and hatching rates, and status of sperm apoptosis in terms of the expression of key regulatory genes were investigated in 11 and 16 month old male L. vannamei...
September 5, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30215807/the-functional-anatomy-of-the-human-spermatozoon-relating-ultrastructure-and-function
#11
David Mortimer
The Internet, magazine articles, and even biomedical journal articles, are full of cartoons of spermatozoa that bear minimal resemblance to real spermatozoa, especially human spermatozoa, and this had led to many misconceptions about what spermatozoa look like and how they are constituted. This review summarizes the historical and current state of knowledge of mammalian sperm ultrastructure, with particular emphasis on and relevance to human spermatozoa, combining information obtained from a variety of electron microscopic (EM) techniques...
September 12, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/30213858/catsper-channels-are-regulated-by-protein-kinase-a
#12
Gerardo Orta, José Luis Vega-Beltran, David Hidalgo, Celia M Santi, Pablo Visconti, Alberto Darszon
Mammalian sperm must undergo capacitation as a preparation for entering into hyperactivated motility, undergoing the acrosome reaction, and acquiring fertilizing ability. One of the initial capacitation events occurs when sperm encounter an elevated HCO3- concentration. This anion activates the atypical adenylyl cyclase Adcy10, increases intracellular cAMP, and stimulates protein kinase A (PKA). Moreover, an increase in intracellular Ca2+ concentration ([Ca2+]i) is essential for sperm capacitation. Although a crosstalk between cAMP-dependent pathways and Ca2+ clearly plays an essential role in sperm capacitation, the connection between these signaling events is incompletely understood...
September 13, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30213537/spermatogenesis-as-a-tool-for-staging-gonad-development-in-the-gonochoric-appendicularian-oikopleura-dioica-fol-1872
#13
Francesca Cima
Oikopleura dioica, the only gonochoric species among appendicularians, has a spematozoon with a mid-piece and a conspicuous acrosome that, during fertilisation, undergoes a reaction forming an acrosomal process. To provide more insight into the spermatogenesis of a holoplanktonic tunicate species that completes its life cycle in three to five days, changes in the testis during individual growth have been examined. Spermatogenesis has been subdivided into seven stages based on ultrastructural features during the formation and organisation of the male gonad and the relationships between its macroscopic anatomy and the events of sperm differentiation...
September 10, 2018: Developmental Biology
https://www.readbyqxmd.com/read/30190490/lysine-acetylation-modulates-mouse-sperm-capacitation
#14
Carla Ritagliati, Guillermina M Luque, Cintia Stival, Carolina Baro Graf, Mariano G Buffone, Dario Krapf
Mammalian sperm are unable to fertilize the egg immediately after ejaculation. To gain fertilization competence, they need to undergo a series of modifications inside the female reproductive tract, known as capacitation. Capacitation involves several molecular events such as phosphorylation cascades, hyperpolarization of the plasma membrane and intracellular Ca2+ changes, which prepare the sperm to develop two essential features for fertilization competence: hyperactivation and acrosome reaction. Since sperm cells lack new protein biosynthesis, post-translational modification of existing proteins plays a crucial role to obtain full functionality...
September 6, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30189323/rna-seq-coupled-to-proteomic-analysis-reveals-high-sperm-proteome-variation-between-two-closely-related-marine-mussel-species
#15
Mónica R Romero, Andrés Pérez-Figueroa, Mónica Carrera, Willie J Swanson, David O F Skibinski, Angel P Diz
Speciation mechanisms in marine organisms have attracted great interest because of the apparent lack of substantial barriers to genetic exchange in marine ecosystems. Marine mussels of the Mytilus edulis species complex provide a good model to study mechanisms underlying species formation. They hybridise extensively at many localities and both pre- and postzygotic isolating mechanisms may be operating. Mussels have external fertilisation and sperm cells should show specific adaptations for survival and successful fertilisation...
September 3, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/30173453/-resveratrol-protects-human-sperm-against-cryopreservation-induced-injury
#16
Shi-Jia Li, Wei-Dong Su, Li-Jun Qiu, Xiong Wang, Juan Liu
Objective: To investigate the effects of resveratrol in the cryopreservation medium on the quality and function of post-thaw sperm. METHODS: Semen samples were obtained from 50 normozoospermic and 50 oligoasthenozoospermic men, liquefied and then cryopreserved in the glycerol-egg yolk-citrate (GEYC) medium with or without 30 μmol/L resveratrol. Sperm motility, viability and acrosome reaction (AR) were examined before and after thawing. Sperm lipid peroxidation and the level of reactive oxygen species (ROS) were measured using commercial malondialdehyde (MDA) and the ROS assay kit...
June 2018: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/30167393/effect-of-catsper-and-hv1-channel-inhibition-on-progesterone-stimulated-human-sperm
#17
Sara Keshtgar, Hamideh Ghanbari, Esmaeel Ghani, Seyed Mostafa Shid Moosavi
Background: Intracellular calcium and proton concentrations are important factors for activating human sperm. Calcium ion (Ca2+ ) enters sperm through voltage-dependent calcium channel of sperm (CatSper). Proton was extruded from sperm through voltage-gated proton channel (Hv1). In the present study, the selective inhibitors of the CatSper and Hv1 channels, NNC 55-0396 (NNC) and zinc ion, respectively, were used to investigate functions of these channels. Methods: Normal semen samples (n=24) were washed and diluted to 20×106 sperm/ml ...
July 2018: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/30166971/itraq-based-analysis-of-sperm-proteome-from-normozoospermic-men-achieving-the-rescue-icsi-pregnancy-after-the-ivf-failure
#18
Xin Liu, Gensheng Liu, Juan Liu, Peng Zhu, Jiahui Wang, Yanwei Wang, Wenting Wang, Ning Li, Xuebo Wang, Chenglin Zhang, Xiaofang Shen, Fujun Liu
Background: In the assisted reproduction, the infertile molecules of spermatozoa from normozoospermic men who underwent the unexplained failure of in vitro fertilization (IVF) due to the lack of sperm binding to the normal zona pellucida, and then achieved pregnancy with the rescue intracytoplasmic sperm injection (R-ICSI) remain unclear. More works are still necessary to explore this male infertile mechanism. Methods: Normozoospermicmen with the IVF pregnancy and normozoospermic men with the R-ICSI pregnancy after the conventional IVF failure were collected...
2018: Clinical Proteomics
https://www.readbyqxmd.com/read/30143014/human-sperm-acrosome-function-assays-are-predictive-of-fertilization-rate-in-vitro-a-retrospective-cohort-study-and-meta-analysis
#19
REVIEW
Fang Xu, Ganggang Guo, Wenbing Zhu, Liqing Fan
OBJECTIVE: To determine whether acrosome function scoring-including acrosomal enzyme (AE) levels and acrosome reaction (AR) results-can predict fertilization rate in vitro. METHODS: We examined the predictive value of acrosomal enzymes (AE) determined by spectrophotometry/N-α-benzoyl-DL-arginine-p-nitroanilide for fertilization rate (FR) in vitro in a retrospective cohort study of 737 infertile couples undergoing IVF therapy. Additionally, a meta-analysis was done for prospective cohort or case-control studies; the following summary measures were reported to expand upon the findings: pooled spearman correlation coefficient (Rs), standardized mean difference (SMD), sensitivity (SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic score (DS), diagnostic odds ratio (DOR), and area under the summary receiver operating characteristic curve (AUC)...
August 24, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/30126978/characterization-of-the-sperm-proteome-and-reproductive-outcomes-with-in-vitro-fertilization-after-a-reduction-in-male-ejaculatory-abstinence-period
#20
Zi-Qi Shen, Bei Shi, Tian-Ren Wang, Jiao Jiao, Xuejun Shang, Qi-Jun Wu, Yi-Ming Zhou, Tie-Feng Cao, Qiang Du, Xiu-Xia Wang, Da Li
Semen samples from men after a short ejaculatory abstinence show improved sperm quality and result in increased pregnancy rates, but the underlying mechanisms remain unclear. Herein, we report that ejaculates from short (1-3 hours) compared with long (3-7 days) periods of abstinence showed increases in motile sperm count, sperm vitality, normal sperm morphology, acrosome reaction capacity, total antioxidant capacity, sperm mitochondrial membrane potential, high DNA stainability, and a decrease in the sperm DNA fragmentation index (P < 0...
August 20, 2018: Molecular & Cellular Proteomics: MCP
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