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Sperm acrosome reaction

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https://www.readbyqxmd.com/read/30109710/genetic-dissection-of-bull-fertility-in-us-jersey-dairy-cattle
#1
F M Rezende, G O Dietsch, F Peñagaricano
The service sire has been recognized as an important factor affecting herd fertility in dairy cattle. Recent studies suggest that genetic factors explain part of the difference in fertility among Holstein sires. The main objective of this study was to dissect the genetic architecture of sire fertility in US Jersey cattle. The dataset included 1.5 K Jersey bulls with sire conception rate (SCR) records and 96 K single nucleotide polymorphism (SNP) markers spanning the whole genome. The analysis included whole-genome scans for both additive and non-additive effects and subsequent functional enrichment analyses using KEGG Pathway, Gene Ontology (GO) and Medical Subject Headings (MeSH) databases...
August 14, 2018: Animal Genetics
https://www.readbyqxmd.com/read/30094771/a-model-for-the-acrosome-reaction-in-mammalian-sperm
#2
Julie Simons, Lisa Fauci
The acrosome reaction is a complex, calcium-dependent reaction that results in an exocytotic event required for successful fertilization of the egg. It has long been thought that the acrosome reaction occurs upon sperm binding to the zona pellucida, a viscoelastic layer surrounding the oocyte. Recent studies have suggested that the reaction may even occur before the sperm encounters the zona, perhaps mediated by progesterone or some other agonist. It has been particularly difficult to understand differences between progesterone-induced and zona-induced reactions experimentally and whether one substance is the more biologically relevant trigger...
August 9, 2018: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/30089752/chimeric-analysis-with-newly-established-egfp-dsred2-tagged-es-cells-identify-hydin-as-essential-for-spermiogenesis-in-mice
#3
Seiya Oura, Haruhiko Miyata, Taichi Noda, Keisuke Shimada, Takafumi Matsumura, Akane Morohoshi, Ayako Isotani, Masahito Ikawa
The CRISPR/Cas9 system can efficiently introduce biallelic mutations in ES cells (ESCs), and its application with fluorescently-tagged ESCs enables phenotype analysis in chimeric mice. We have utilized ESCs that express EGFP in the cytosol and acrosome [EGR-G101 129S2 x (CAG/Acr-EGFP) B6] in previous studies; however, the EGFP signal in the sperm cytosol is weak and the signal in the acrosome is lost after the acrosome reaction, precluding analysis between wild type and ESC derived spermatozoa. In this study, we established an ESC line from RBGS (Red Body Green Sperm) transgenic mice [B6D2-Tg(CAG/Su9-DsRed2, Acr3-EGFP)RBGS002Osb] whose spermatozoa exhibit green fluorescence in the acrosome and red fluorescence in the mitochondria within the flagellar midpiece that is retained after the acrosome reaction...
August 9, 2018: Experimental Animals
https://www.readbyqxmd.com/read/30063019/-capacitation-and-acrosome-reaction-are-associated-with-changes-in-sialic-acid-location-and-head-morphometry-in-human-sperm
#4
Paula Sáez-Espinosa, Stéphanie Torrijo-Boix, Natalia Huerta-Retamal, Manuel Avilés, Jon Aizpurua, Alejandro Romero, María José Gómez-Torres
OBJECTIVE: Assess changes in sialic acid distribution during capacitation and acrosome reaction processes, and evaluate head sperm morphometrics modifications in these physiological conditions in human sperm. MATERIAL AND METHOD: In this prospective study, we included 6 normozoospermics sperm samples. Sialic acid distribution was evaluated by Wheat germ agglutinin lectin in different physiological conditions: before, after capacitation and after acrosome reaction...
January 2018: Revista Internacional de Andrología
https://www.readbyqxmd.com/read/30034181/insights-into-ph-regulatory-mechanisms-in-mediating-spermatozoa-functions
#5
REVIEW
Abhishek Kumar Mishra, Akshay Kumar, Dilip Kumar Swain, Sarvajeet Yadav, Rajesh Nigam
Regulation of pH in spermatozoa is a complex and dynamic process as sperm cells encounter different pH gradients during their journey from testes to the site of fertilization in female genital tract. The precise regulations of pH in sperm cells regulate the sperm functions such as motility, hyperactivity, capacitation, and acrosome reaction. Electrophysiological, pharmacological, and molecular studies have revealed the presence of different ion channels and exchanger systems which regulate intracellular pH in sperm cells as well as regulate sperm functions...
June 2018: Veterinary World
https://www.readbyqxmd.com/read/30033774/lifestyle-and-demographic-factors-associated-with-human-semen-quality-and-sperm-function
#6
Xiao Shi, Carol Pui Shan Chan, Tarah Waters, Ling Chi, David Yiu Leung Chan, Tin-Chiu Li
In this study, we sought to investigate the effect of lifestyle and demographic factors on classic and functional semen parameters. Three hundred and twenty-eight subjects who underwent semen analysis were recruited. Routine SA, sperm vitality, acrosome reaction (AR) assay and sperm DNA fragmentation index (DFI) were analyzed. Demographic and lifestyle information, including (1) BMI, (2) current smoking and alcohol drinking frequency, (3) sleep habits, (4) daily fluid intake, (5) weekly meat intake, (6) sports frequency, (7) trouser cell phone use, (8) age, and (9) abstinence time, were collected...
July 23, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/30015247/characterization-and-potential-roles-of-calretinin-in-rodent-spermatozoa
#7
Cindy Dressen, Beat Schwaller, Grégory Vegh, Fabienne Leleux, David Gall, Philippe Lebrun, Pascale Lybaert
Calretinin has been detected in various excitable cells but the presence and putative roles of such a calcium-binding protein has never been characterized in sperm. Epididymal spermatozoa were collected from C57Bl6 (wild-type, WT) or calretinin knockout (CR-/- ) mice and Wistar rats. A specific staining for calretinin was detected by immunofluorescence in the principal piece of the flagellum, both in WT mouse and rat spermatozoa. Western blots confirmed the expression of calretinin in rat and WT spermatozoa as well as its absence in CR-/- mice...
June 1, 2018: Cell Calcium
https://www.readbyqxmd.com/read/30009954/rosmarinic-acid-compromises-human-sperm-functions-by-an-intracellular-ca-2-concentration-related-mechanism
#8
Meng-Ge Lv, Wen-Qiong Chen, Shi-Qi Weng, Hou-Yang Chen, Yi-Min Cheng, Tao Luo
Rosmarinic acid (RA), a natural phenolic ester, is cytoprotective for male reproduction in animal models. The present study investigated the in vitro actions of RA on human sperm functions. Human sperm were exposed to 1, 10, 100, and 1000 μM RA in vitro and sperm functions were examined. The results showed that although RA did not affect human sperm viability, RA at 10-1000 μM dose-dependently reduced sperm motility, penetration ability, capacitation, and spontaneous acrosome reaction. In addition, the intracellular Ca2+ concentration ([Ca2+ ]i ), which serve as a key regulator of sperm function, was decreased by RA (10-1000 μM) in a dose-dependent manner...
July 20, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/30009140/zinc-is-an-essential-element-for-male-fertility-a-review-of-zn-roles-in-men-s-health-germination-sperm-quality-and-fertilization
#9
REVIEW
Ali Fallah, Azadeh Mohammad-Hasani, Abasalt Hosseinzadeh Colagar
Zinc (Zn) is the second most abundant trace element in human, which can't be stored in the body, thus regular dietary intake is required. This review explained the physiological and pathogenesis roles of zinc in men's health and its potentials in germination, quality of sperm, and fertilization. Our investigation showed that Zn contained many unique properties in human, especially males. The antioxidant quality is one of them. Also, the increased reactive oxygen species levels in the seminal plasma of men who are both infertile and smokers influence the Zn content of seminal plasma in a way that physiology of spermatozoa can be affected as well...
April 2018: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/29991051/5-amp-activated-protein-kinase-regulates-goat-sperm-functions-via-energy-metabolism-in-vitro
#10
Zhendong Zhu, Rongnan Li, Gongzhen Ma, Wenjing Bai, Xiaoteng Fan, Yinghua Lv, Jun Luo, Wenxian Zeng
BACKGROUND/AIMS: ATP is essential for mammalian sperm to survive and maintain fertilizing capacity. AMP-activated protein kinase (AMPK) is a sensor of cellular energy status. The aims of the present study were to explore the localization of AMPK in goat sperm and to investigate whether and how AMPK regulates sperm functions in vitro. METHODS: Sperm were treated with AMPK modulators (AICAR, metformin and Compound C) during incubation. Sperm motility was assessed with a computer-assisted spermatozoa analysis system (CASA)...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29969085/human-cumulus-cell-sensitivity-to-vitrification-an-ultrastructural-study
#11
Neda Taghizabet, Mohammad Ali Khalili, Fatemeh Anbari, Azam Agha-Rahimi, Stefania Annarita Nottola, Guido Macchiarelli, Maria Grazia Palmerini
SummaryCumulus cells (CCs) play an important role in the regulation of female gamete development, meiotic maturation, oocyte-sperm interaction, capacitation and acrosome reaction. However, their role in maintaining oocyte competence after vitrification is unclear as controversial data on their protecting action against oocyte cryoinjuries are available. Here we described the effects of vitrification on the ultrastructure of human CCs collected from women undergoing assisted reproductive technologies (ARTs)...
June 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29954238/monitoring-dimeric-status-of-izumo1-during-the-acrosome-reaction-in-living-spermatozoon
#12
Naokazu Inoue, Ikuo Wada
The acrosome reaction (AR) is indispensable for successful spermatozoon-oocyte fusion. Recent studies have indicated that sperm IZUMO1 gradually gathers in the equatorial segment (EQ), which is the initiation site of sperm-egg fusion, after the AR. In addition, by examining the binding process of oocytes and Izumo1-expressing cultured cells to reconstitute the early steps of fertilization, we previously demonstrated that robust IZUMO1-dependent adhesion specifically occurs at the contact site along with the dimerization of IZUMO1...
July 25, 2018: Cell Cycle
https://www.readbyqxmd.com/read/29942931/testicular-histopathology-and-phosphorylated-protein-changes-in-mice-with-diabetes-induced-by-multiple-low-doses-of-streptozotocin-an-experimental-study
#13
Apichakan Sampannang, Supatcharee Arun, Jaturon Burawat, Wannisa Sukhorum, Sitthichai Iamsaard
Background: The streptozotocin (STZ)-induced diabetic model is widely used to evaluate the adverse effects of diabetes mellitus (DM) on spermatogenesis and testicular steroidogenesis. However, the actual mechanism of sub/infertility in DM males needs to be elucidated. Objective: To conduct a detailed examination of the testicular histopathology, sperm acrosome reaction (AR) status, and tyrosine-phosphorylated protein expression in the testis of male mice induced with STZ...
April 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29921626/activated-farnesoid-x-receptor-fxr-expressed-in-human-sperm-alters-its-fertilizing-ability
#14
Rocco Malivindi, Marta Santoro, Daniela De Rose, Salvatore Panza, Serena Gervasi, Vittoria Rago, Saveria Aquila
The farnesoid X receptor alpha (FXR) is a bile acid sensor activated by binding to endogenous bile acids including chenodeoxycholic acid (CDCA). Although, FXR is expressed in male reproductive tissue, the relevance of the receptor on reproduction is scarcely known. Here we demonstrated the FXR presence and its action on several human sperm features. Western blot and Immunofluorescence assays evidenced the FXR expression in human spermatozoa and the localization in the middle piece. CDCA increasing concentrations and GW4064, synthetic ligand of FXR, were used to study the FXR influence on sperm motility, survival, capacitation, acrosome reaction and on glucose as well as lipid metabolism...
June 19, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29913423/effect-of-aminopeptidase-n-on-functions-and-fertility-of-mouse-spermatozoa-in-vitro
#15
Amena Khatun, Kyu-Ho Kang, Do-Yeal Ryu, Md Saidur Rahman, Woo-Sung Kwon, Myung-Geol Pang
Aminopeptidase N (APN) is defined as a multifunctional enzyme, which regulate cellular physiology of a wide variety of cells in human. Earlier studies reported that mammalian semen shares this common enzyme as a major protein of seminal plasma that has correlation with male fertility, while the regulatory mechanisms of APN in spermatozoa are still far from being well understood. Present study was designed to investigate the role of APN in biological and chemical functions of spermatozoa using an in vitro antagonistic approach...
June 12, 2018: Theriogenology
https://www.readbyqxmd.com/read/29880644/lrrc8-vrac-anion-channels-are-required-for-late-stages-of-spermatid-development-in-mice
#16
Jennifer C Lück, Dmytro Puchkov, Florian Ullrich, Thomas J Jentsch
Spermatogenesis is a highly complex developmental process that occurs primarily in seminiferous tubules of the testes and requires additional maturation steps in the epididymis and beyond. Mutations in many different genes can lead to defective spermatozoa and hence to male infertility. Some of these genes encode for ion channels and transporters that play roles in various processes such as cellular ion homeostasis, signal transduction, sperm motility, and the acrosome reaction. Here we show that germ cell-specific, but not Sertoli cell-specific, disruption of Lrrc8a leads to abnormal sperm and male infertility in mice...
July 27, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29861341/nitric-oxide-in-frozen-thawed-equine-sperm-effects-on-motility-membrane-integrity-and-sperm-capacitation
#17
André F C de Andrade, Rubens P Arruda, Mariana A Torres, Naira C G Pieri, Ticiano G Leite, Eneiva Carla C Celeghini, Leticia Z Oliveira, Thayna P Gardés, Maria Clara C Bussiere, Daniela F Silva
Nitric oxide (NO) is a reactive nitrogen species (RSN) that, over the years, has been shown to be integrated with biological and physiological events, including reproductive processes. NO can affect the functionality of spermatozoa through free radical scavenging, deactivating and inhibiting the production of superoxide anions (O2 .- ). However, the role of NO in mammalian spermatozoa physiology seems paradoxical. The aim of this study was to investigate the effects of NO on motility, hyperactivation, membrane integrity, peroxidation, and capacitation in cryopreserved equine sperm...
May 22, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29807255/improved-embryo-development-using-high-cysteamine-concentration-during-ivm-and-sperm-co-culture-with-cocs-previous-to-icsi-in-bovine
#18
Natalia Gabriela Canel, Mariana Suvá, Romina Jimena Bevacqua, María Elena Arias, Ricardo Felmer, Daniel Felipe Salamone
In contrast to other species, intracytoplasmic sperm injection (ICSI) in bovine remains inefficient, resulting in low embryo developmental rates. It is unclear whether such inefficiency is due to the poor response of bovine ooplasms to the injection stimulus, or to the inability of bull sperm to induce oocyte activation. In order to facilitate these events, two strategies were assessed: the use of high concentration of cysteamine [Cys] during IVM; and the selection of sperm attached to cumulus cells after incubation with COCs for ICSI...
September 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/29788183/membrane-rafts-regulate-sperm-acrosome-reaction-via-camp-dependent-pathway-in-chickens-gallus-gallus-domesticus
#19
Chathura Priyadarshana, Atsushi Tajima, Naoto Ishikawa, Atsushi Asano
Both transcriptionally and translationally inactive sperm need preassembled pathways into specific cellular compartments to function. Although initiation of the acrosome reaction (AR) involves several signaling pathways including protein kinase A (PKA) activation, how these are regulated remains poorly understood in avian sperm. Membrane rafts are specific membrane regions enriched in sterols and functional proteins and play important roles in diverse cellular processes, including signal transduction. Our recent studies on chicken sperm demonstrated that membrane rafts exist and play a role in multistage fertilization...
May 18, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29783741/serine-protease-inhibitor-serpine2-reversibly-modulates-murine-sperm-capacitation
#20
Sheng-Hsiang Li, Yuh-Ming Hwu, Chung-Hao Lu, Ming-Huei Lin, Ling-Yu Yeh, Robert Kuo-Kuang Lee
SERPINE2 (serpin peptidase inhibitor, clade E, member 2), predominantly expressed in the seminal vesicle, can inhibit murine sperm capacitation, suggesting its role as a sperm decapacitation factor (DF). A characteristic of DF is its ability to reverse the capacitation process. Here, we investigated whether SERPINE2 can reversibly modulate sperm capacitation. Immunocytochemical staining revealed that SERPINE2 was bound onto both capacitated and uncapacitated sperm. It reversed the increase in BSA-induced sperm protein tyrosine phosphorylation levels...
May 19, 2018: International Journal of Molecular Sciences
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