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Sperm acrosome reaction

Tereza Bosakova, Antonin Tockstein, Natasa Sebkova, Ondrej Simonik, Hana Adamusova, Jana Albrechtova, Tomas Albrecht, Zuzana Bosakova, Katerina Dvorakova-Hortova
17β-estradiol (estradiol) is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation-called capacitation-and sperm⁻egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis...
December 12, 2018: International Journal of Molecular Sciences
Zeinab Fereshteh, Pradeepthi Bathala, Deni S Galileo, Patricia A Martin-DeLeon
Extracellular vesicles (EVs) were isolated by ultracentrifugation of vaginal luminal fluid (VLF) from superovulated mice and identified for the first time using transmission electron microscopy. Characterized by size and biochemical markers (CD9 and HSC70), EVs were shown to be both microvesicular and exosomal and were dubbed as "Vaginosomes" (VGS). Vaginal cross-sections were analyzed to visualize EVs in situ: EVs were present in the lumen and also embedded between squamous epithelial and keratinized cells, consistent with their endogenous origin...
December 7, 2018: Journal of Cellular Physiology
Wen Chen, Shi-Qi Weng, Meng-Ge Lv, Wen-Qiong Chen, Zhuo-Fei Bi, Hou-Yang Chen, Tao Luo, Hai Hu, Wen-Bo Liao
BACKGROUND/AIMS: β-Elemene is a bioactive sesquiterpene compound that exhibits a potent anti-tumor effect and is used in various clinical applications. However, little is known about its effect on the male reproductive system. The objective of this study was to investigate the in vitro actions of β-elemene on human sperm function and elucidate the underlying mechanism. METHODS: The cytotoxicity of β-elemene toward MCF-10A, MDA-MD-231, and A549 cells was evaluated with cell proliferation and colony formation assays...
2018: Cellular Physiology and Biochemistry
Carla Moros-Nicolás, Sophie Fouchécourt, Ghylène Goudet, Philippe Monget
Oviductal proteins play an important role in mammalian fertilization, as proteins from seminal fluid. However, in contrast with the latter, their phylogenetic evolution has been poorly studied. Our objective was to study in 16 mammals the evolution of 16 genes that encode oviductal proteins involved in at least one of the following steps: (1) sperm-oviduct interaction, (2) acrosome reaction, and/or (3) sperm-zona pellucida interaction. Most genes were present in all studied mammals. However, some genes were lost along the evolution of mammals and found as pseudogenes: annexin A5 (ANXA5) and deleted in malignant brain tumor 1 (DMBT1) in tarsier; oviductin (OVGP1) in megabat; and probably progestagen-associated endometrial protein (PAEP) in tarsier, mouse, rat, rabbit, dolphin, and megabat; prostaglandin D2 synthase (PTGDS) in microbat; and plasminogen (PLG) in megabat...
December 2018: Journal of Molecular Evolution
Guowei Zhang, Wang Yang, Peng Zou, Fan Jiang, Yingfei Zeng, Qing Chen, Lei Sun, Huan Yang, Niya Zhou, Xiaogang Wang, Jinyi Liu, Jia Cao, Ziyuan Zhou, Lin Ao
STUDY QUESTION: In addition to sperm motility, which major biological characteristics of sperm fertility potential are associated with mitochondrial functionality? SUMMARY ANSWER: Sperm fertilization capacities, including acrosin activity, acrosome reaction (AR) capability and chromatin integrity, are related to the mitochondria functionality as evaluated by the mitochondrial membrane potential (MMP). WHAT IS KNOWN ALREADY: Correlative studies suggest a potential role of sperm MMP in predicting sperm fertilization ability and ensuring sperm motility...
November 14, 2018: Human Reproduction
Nana Wen, Meng-Fei Yu, Jing Liu, Congli Cai, Qing-Hua Liu, Jinhua Shen
Calcium signalling is critical for successful fertilization. In spermatozoa, capacitation, hyperactivation of motility and acrosome reactions are all mediated by increases in intracellular Ca2+. Our previous reports have shown that deficiency of MTMR14, a novel phosphoinositide phosphatase, induces a muscle disorder by disrupting Ca2+ homeostasis. Recently, we found that MTMR14 is also expressed in the testes; however, whether deficiency of MTMR14 in the testes also alters the Ca2+ concentration and impairs male fertility remains entirely unknown...
2018: PloS One
Juan Liu, Wenting Wang, Xin Liu, Xuebo Wang, Jiahui Wang, Yanwei Wang, Ning Li, Xiong Wang
OBJECTIVE: To investigate the potential effects of TAT-PRDX2 protein supplementation to the cryopreservation medium on post-thaw sperm quality and function. DESIGN: In vitro prospective study. SETTING: Medical university hospital. PATIENT(S): Fifty normozoospermic, 50 asthenozoospermic, and 50 oligoasthenozoospermic men undergoing semen analysis for couple infertility. INTERVENTION(S): Each semen sample was divided into three aliquots: fresh, cryopreserved control (without additive), and cryopreserved with TAT-PRDX2 protein...
November 2018: Fertility and Sterility
A Kumaresan, Anders Johannisson, Patrice Humblot, Ann-Sofi Bergqvist
This study was conducted to investigate the complex interactions between oviducts and cryopreserved spermatozoa. Herein we report the dynamic changes in bull sperm functions during in vitro incubation with bovine estrus and luteal oviductal fluid. Frozen-thawed bull spermatozoa was incubated either in non-capacitating medium, capacitating medium, non-capacitating medium containing 20% v/v estrus oviductal fluid or non-capacitating medium containing 20% v/v luteal oviductal fluid for 6 h at 38 °C under 5% CO2 ...
January 15, 2019: Theriogenology
Pamela Tello-Mora, Leticia Hernández-Cadena, Jeimy Pedraza, Esther López-Bayghen, Betzabet Quintanilla-Vega
BACKGROUND: Traditional semen parameters have shown little to none predictive value for fertilization and blastocyst viability for a successful pregnancy. Therefore, the purpose of this study was to explore the usefulness of incorporating the acrosome reaction (AR) and chromatin integrity to conventional semen analysis to individually predict the fertile potential of sperm samples. METHODS: A cross-sectional study was conducted in 69 participants undergoing IVF using oocyte donation...
October 19, 2018: Reproductive Biology and Endocrinology: RB&E
Qianrong Qi, Yifan Yang, Kailin Wu, Qingzhen Xie
Recent studies revealed that TMEM16A is involved in several reproductive processes, including ovarian estrogen secretion and ovulation, sperm motility and acrosome reaction, fertilization and myometrium contraction. However, little is known about the expression and function of TMEM16A in embryo implantation and decidualization. In this study, we focused on the expression and regulation of TMEM16A in mouse uterus during early pregnancy. We found that TMEM16A is upregulated in uterine endometrium in response to embryo implantation and decidualization...
October 2, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Chizuru Ito, Kenji Yamatoya, Keiichi Yoshida, Lisa Fujimura, Hajime Sugiyama, Akiko Suganami, Yutaka Tamura, Masahiko Hatano, Kenji Miyado, Kiyotaka Toshimori
A number of sperm proteins are involved in the processes from gamete adhesion to fusion, but the underlying mechanism is still unclear. Here, we established a mouse mutant, the EQUATORIN-knockout (EQTN-KO, Eqtn−/−) mouse model and found that the EQTN-KO males have reduced fertility and sperm–egg adhesion, while the EQTN-KO females are fertile. Eqtn−/− sperm were normal in morphology and motility. Eqtn−/−-Tg (Acr-Egfp) sperm, which were produced as the acrosome reporter by crossing Eqtn−/− with Eqtn+/+-Tg(Acr-Egfp) mice, traveled to the oviduct ampulla and penetrated the egg zona pellucida of WT females...
October 1, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Gabriela Hernández-Silva, Marta Durand, Fernando Larrea, Mayel Chirinos
When levonorgestrel (LNG) is given for emergency contraception during the follicular phase, it not only inhibits or delays ovulation, but also induces changes in endometrial secretions that modulate sperm functionality. In order to characterize the female reproductive tract secreted molecules that may affect human spermatozoa, we analyzed changes in the protein content of uterine flushings obtained from women during the periovulatory phase of a control and a LNG-treated menstrual cycle. Lectin affinity analysis and 2D gel electrophoresis of uterine samples showed changes in protein glycosylation patterns and the presence of 31 differentially expressed proteins (8 upregulated and 23 downregulated)...
September 12, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Francisco A García-Vázquez, C Soriano-Úbeda, R Laguna-Barraza, M José Izquierdo-Rico, Felipe A Navarrete, Pablo E Visconti, A Gutiérrez-Adán, P Coy
Besides its fibrinolytic function, the plasminogen-plasmin (PLG-PLA) system is also involved in fertilisation, where plasminogen activators bind to plasminogen to produce plasmin, which modulates sperm binding to the zona pellucida. However, controversy exists, depending on the species, concerning the role of the different components of the system. This study focused its attention on the role of the PLG-PLA system on fertilisation in the mouse with special attention to tissue plasminogen activator (tPA). The presence of exogenous plasminogen reduced in vitro fertilisation (IVF) rates and this decline was attenuated by the presence of plasmin inhibitors in combination with plasminogen...
October 10, 2018: Reproduction, Fertility, and Development
Asghar Beigi Harchegani, Ali Irandoost, Mahdiyeh Mirnamniha, Hamid Rahmani, Eisa Tahmasbpour, Alireza Shahriary
Calcium (Ca) is a significant element that acts as an intracellular second messenger. It is necessary for many physiological processes in spermatozoa including spermatogenesis, sperm motility, capacitation, acrosome reaction and fertilization. Although influences of Ca deficiency on sperm function and male infertility have been widely studied, mechanisms for these abnormalities are not well considered. Poor sperm motility, impairment of chemotaxis, capacitation, acrosome reaction and steroidogenesis are the major mechanisms by which Ca deficiency induces male infertility...
January 2019: International Journal of Fertility & Sterility
Ejaz Ahmad, Zahid Naseer, Uğur Uçan, İlker Serin, Ahmet Ceylan, Melih Aksoy
The study was conducted to evaluate the seasonal dynamics in the sperm acrosome reaction (AR), osmotic tolerance and serum testosterone concentration in rams. Blood and semen samples were collected from six mature rams during the winter, spring, summer and autumn seasons. The AR in sperm samples was induced by using lysophosphatidylcholine (LPC) and calcium ionophore (CI). The AR was monitored at various incubation times (15, 30, 60, 120 and 180 min) post-induction. To determine the osmotic tolerance through hypo-osmotic swelling test (HOST) the sperm were exposed to different osmotic conditions (50, 100, 290, 500 and 1000 mOsm/L)...
November 2018: Animal Reproduction Science
Zahid Naseer, Ejaz Ahmad, Hande Sultan Şahiner, Erkmen Tuğrul Epikmen, Muhammad Fiaz, Muhamad Rizwan Yousuf, Shahzad Akbar Khan, İlker Serin, Ahmet Ceylan, Melih Aksoy
This study focused to determine beneficial impact of feeding quercetin supplemented diet on semen quality in summer heat imposed rabbits. Twelve heat stressed (HS) adult rabbits bucks were either fed with basal diet (HS; n = 06) or quercetin supplemented diet (QU-HS; n = 06) for a period of 56 days. Semen samples were collected and evaluated for volume, osmolality, morphology, concentration, motility, motion kinetics, viability, acrosome integrity, mitochondrial potential, and seminal plasma MDA level...
September 15, 2018: Theriogenology
M Efrat, A Stein, H Pinkas, H Breitbart, R Unger, R Birk
BACKGROUND: Sperm capacitation is essential for proper fertilization and is associated with increased sperm hyperactivity (HA) and acrosome reaction (AR). For successful fertilization, AR timing is critical; accordingly, early spontaneous AR may not facilitate fertilization. Paraoxonase 1 (PON1) possesses antioxidant properties which affect sperm capacitation. The association between PON1, semen parameters, and capacitation is not fully understood. OBJECTIVE: To study PON1 activity in relation to human sperm hyperactivity and AR...
September 17, 2018: Andrology
Tiane Ferreira de Castro, Graciela Quintana Saalfeld, Antonio Sergio Varela, Francine Ferreira Padilha, Klebson Silva Santos, Diego Martins Pires, Jessica Ribeiro Pereira, Carine Dahl Corcini, Elton Pinto Colares
The present study aims to evaluate the influence of triphenyltin (TPT) exposure on reproductive physiology on Calomys laucha species, since this species inhabits regions susceptible to exposure to this contaminant. Animals exposed to the highest dose (10.0 mg/kg) presented signs of severe intoxication in only 7 days of exposure, demonstrating a higher sensitivity of this species to triphenyltin. The 10.0 mg TPT/kg dose was analyzed separately for short-term exposure and results suggest that exposure to this dose was severely detrimental to sperm activity...
November 2018: Chemosphere
Wei Shi, Shanjie Zha, Lihua Hu, Xianke Huang, Jiongming Zhang, Maocang Yan, Guangxu Liu
Although the effects of age on the reproductive performance of various invertebrates, including white shrimp Litopenaeus vannamei are increasingly well documented, the mechanisms manifesting these impacts remain poorly understood. To ascertain the mechanisms of age on reproductive performance, the sperm quality, intracellular contents of Ca2+ , insemination and hatching rates, and status of sperm apoptosis in terms of the expression of key regulatory genes were investigated in 11 and 16 month old male L. vannamei...
November 2018: Animal Reproduction Science
David Mortimer
The Internet, magazine articles, and even biomedical journal articles, are full of cartoons of spermatozoa that bear minimal resemblance to real spermatozoa, especially human spermatozoa, and this had led to many misconceptions about what spermatozoa look like and how they are constituted. This review summarizes the historical and current state of knowledge of mammalian sperm ultrastructure, with particular emphasis on and relevance to human spermatozoa, combining information obtained from a variety of electron microscopic (EM) techniques...
December 1, 2018: Molecular Human Reproduction
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