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Sperm maturation

C Jean, F Haghighirad, Y Zhu, M Chalbi, A Ziyyat, E Rubinstein, C Gourier, P Yip, J P Wolf, J E Lee, C Boucheix, V Barraud-Lange
STUDY QUESTION: Is JUNO protein present at the surface membrane of human oocytes and involved in the fertilisation process? SUMMARY ANSWER: JUNO protein is expressed on the plasma membrane of human oocytes and its inhibition by a monoclonal antibody completely blocks gamete fusion. WHAT IS KNOWN ALREADY: Fusion of gamete membranes is the culminating event of the fertilisation process, but its molecular mechanisms are poorly understood. Until now, three molecules have been shown to be essential: CD9 tetraspanin in the oocyte, Izumo1 protein on the sperm and Juno, its corresponding receptor on the oocyte...
December 5, 2018: Human Reproduction
Xiaoning Zhang, Peng Zhang, Dandan Song, Suping Xiong, Hongyan Zhang, Jianbo Fu, Fengxin Gao, Houyang Chen, Xuhui Zeng
Increasing studies have shown that specific mRNAs and miRNAs expressed in mature sperm may be related to sperm motility. However, the expression profiles and roles of lncRNAs in sperm remain unknown. In the present study, numerous lncRNAs were identified in human sperm, and some lncRNAs were expressed exclusively in sperm and testis. Compared with normal sperm, the lncRNA expression profiles in asthenozoospermia (AZS) sperm showed significant differences. Gene ontology and pathway analyses showed that function of differentially expressed lncRNA targets and mRNAs between AZS and normal sperm were closely-linked with many processes involved in spermatogenesis and sperm function...
December 4, 2018: Biology of Reproduction
Volodymyr Bondarenko, Miroslav Blecha, Tomas Policar
Sexually mature males (BW = 1600 ± 150 g and TL = 235 ± 30 mm) of northern pike (Esox lucius L.) were randomly selected from a pond to record changes in their sperm quality parameters (spermatozoa morphology, sperm volume, density, and motility parameters) during the spawning season. The morphological and motility parameters changed significantly during the reproductive season with following trends. Only, head width was not changed during the spawning season. The longest spermatozoa and its flagellar length were found at the middle of spawning period (TL = 38...
December 4, 2018: Fish Physiology and Biochemistry
Amanda Setti, Gabriela Halpern, Daniela Braga, Rita Figueira, Assumpto Iaconelli, Edson Borges
PURPOSE: To study the association between body mass index (BMI), waist circumference (WC), waist-to-hip ratio (WHR), and waist-to-height ratio (WHtR), and response to controlled ovarian stimulation, semen quality, and intracytoplasmic sperm injection (ICSI) outcomes. METHODS: This prospective observational study included data from 402 couples undergoing ICSI between January/2010-September/2016. Participants had their weight, height, waist, and hip measured before ICSI treatment...
December 5, 2018: Journal of Assisted Reproduction and Genetics
Carolina Briones, José J Nuñez, Montse Pérez, Daniela Espinoza-Rojas, Cristian Molina-Quiroz, Ricardo Guiñez
Perumytilus purpuratus is a marine mussel considered a bioengineer species with a broad distribution in the Pacific and Atlantic coast of South America. Studies have shown two geographically and genetically differentiated subpopulations at molecular level and in sperm morphological traits. To open avenues for molecular research on P. purpuratus , a global de novo transcriptome from gonadal tissue of mature males was sequenced using the Illumina platform. From a total of 126.38 million reads, 37,765 transcripts were successfully annotated...
2018: Journal of Genomics
Guillermo Carvajal, Nicolás Gastón Brukman, Mariana Weigel Muñoz, María A Battistone, Vanesa A Guazzone, Masahito Ikawa, Miyata Haruhiko, Livia Lustig, Sylvie Breton, Patricia S Cuasnicu
Epididymal Cysteine Rich Secretory Proteins 1 and 4 (CRISP1 and CRISP4) associate with sperm during maturation and play different roles in fertilization. However, males lacking each of these molecules individually are fertile, suggesting compensatory mechanisms between these homologous proteins. Based on this, in the present work, we generated double CRISP1/CRISP4 knockout (DKO) mice and examined their reproductive phenotype. Our data showed that the simultaneous lack of the two epididymal proteins results in clear fertility defects...
December 3, 2018: Scientific Reports
Mustafa Numan Bucak, Mustafa Bodu, Nuri Başpınar, Şükrü Güngör, Pınar İli, Begimay Acibaeva, Tohid Rezaei Topraggaleh, Şükrü Dursun
Objective: The purpose of the present study was to assess the effects of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Materials and Methods: In this experimental study, sixty ejaculates from six mature Merino rams were used. In experiment 1, the ejaculates were diluted in base extender contained ellagic acid at 0 (control), 0.5, 1, and 2 mM. In experiment 2, ebselen at 0 (control), 10, 20, and 40 μM were added to the extender...
April 2019: Cell Journal
Osamu Kobayashi, Shigekazu Tomizuka, Shota Shimizu, Ryuichiro Machida
Spermatogenesis in the freshwater pearl mussel Margaritifera laevis was investigated using light and electron microscopy. The testes of M. laevis are composed of numerous acini. We observed type A spermatogonia, large cells of irregular shape, solely near the acinus basal lamina. Type A spermatogonia proliferate and become type B spermatogonia, which are also irregular in shape and form clusters of germ cells of the same developmental stage. The numerous clusters differ with respect to developmental stage and are arranged randomly along the acinus periphery...
December 2018: Tissue & Cell
Artur Wdowiak, Paweł A Mazurek, Anita Wdowiak, Iwona Bojar
OBJECTIVES: Abnormalities in the timing and course of spermatozoa capacitation and hyperactivation underlie common pathologies related to male infertility. Recent data shows that low frequency electromagnetic waves may influence cell membrane potential and permeability. It is therefore possible that low frequency electromagnetic waves could affect the maturation and motility processes of spermatozoa. The 43-kHz wave generator was used for modeling the impact of environmental exposure to low frequency electromagnetic radiation on human sperm...
November 28, 2018: International Journal of Occupational Medicine and Environmental Health
Charalampos S Siristatidis, Abha Maheshwari, Dennis Vaidakis, Siladitya Bhattacharya
BACKGROUND: Polycystic ovarian syndrome (PCOS) occurs in 4% to 7% of all women of reproductive age and 50% of women presenting with subfertility. Subfertility affects 15% to 20% of couples trying to conceive. A significant proportion of these women ultimately need assisted reproductive technology (ART). In vitro fertilisation (IVF) is one of the assisted reproduction techniques employed to raise the chances of achieving a pregnancy. For the standard IVF technique, stimulating follicle development and growth before oocyte retrieval is essential, for which a large number of different methods combining gonadotrophins with a gonadotrophin-releasing hormone (GnRH) agonist or antagonist are used...
November 15, 2018: Cochrane Database of Systematic Reviews
Jose V Medrano, Saturnino Luján, María Del Mar Andrés, Antonio Pellicer
Some treatments for any cancer therapy and hematological diseases may have gonadotoxic side effects that can result in infertility, and thus sperm cryopreservation is routinely offered to patients as the strategy to preserve their fertility. However, there are many cases where sperm banking cannot be applied, as is the case of pre-pubertal cancer patients and others unable to produce mature gametes at the moment of diagnosis. Regarding this, recent breakthroughs have gained public attention to the fertility preservation options that Regenerative Medicine can offer to these patients...
November 23, 2018: Revista Internacional de Andrología
Thi Mong Diep Nguyen
Motility is an essential function of the mature male gamete and is widely used as an indicator of semen quality. Knowledge of signal transduction pathways regulating sperm motility is therefore important to gain insight into new methods to increase the fertilizing ability of semen samples, especially those subjected to biotechnological processes such as cryopreservation. In this article, we will briefly describe the roles of several signaling pathways involved in the motility of fowl sperm as compared to mammalian sperm...
November 23, 2018: Poultry Science
Fumie Magata, Kanami Tsuchiya, Haruna Okubo, Atsushi Ideta
Reduction in oocyte quality is a major factor responsible for declining fertility associated with maternal aging in cows. The objective of the present study was to determine whether intracytoplasmic sperm injection (ICSI) could increase the efficiency of embryo production in older cows. We used cows aged 30 to 50 months or >120 months, which were defined as young or aged, respectively. The distribution of cortical granules in oocytes was affected by age as older cows had lower proportion of oocytes with mature cytoplasm containing evenly dispersed cortical granules compared to young cows...
November 26, 2018: Journal of Veterinary Medical Science
Melissa Carvajal-Serna, Felipe Torres-Ruda, Jaime Antonio Cardozo, Henry Grajales-Lombana, José Álvaro Cebrián-Pérez, Teresa Muiño-Blanco, Rosaura Pérez-Pé, Adriana Casao
In temperate climates, photoperiod and melatonin regulate ram reproduction, modulating hormonal secretions, sperm quality, and seminal plasma composition. Information on the effect of an equatorial photoperiod (12L:12D) on ram reproduction, however, is scarce, and no data on hormonal concentrations and antioxidant enzyme activity in seminal plasma have been reported. Thus, the variation was investigated of melatonin and its relationship with testosterone and antioxidant enzyme activity in the seminal plasma of three sheep breeds in Colombia, when there was a consistent photoperiod during two dry and two rainy seasons per year...
November 16, 2018: Animal Reproduction Science
Keisuke Matsushima, Tomohiro Watanabe, Ken Sasaki
Dopamine is a potential integrator between the central nervous system and reproductive system in insects. To test for a possible action of dopamine on the male reproductive organ via hemolymph in honey bees, relative expression levels of dopamine receptor genes and second messenger levels responding to dopamine in the reproductive organ were quantified. Protein content of the three parts of the reproductive organ (testes, seminal vesicles, and mucus glands) differed depending on the age of bees: the protein content of the testes decreased, whereas that of the seminal vesicles and mucus glands increased as males aged...
November 22, 2018: Journal of Insect Physiology
Daniela R Chavez, Angela K Snow, Joseph R Smith, Gillian M Stanfield
The development of highly differentiated sperm cells specialized for navigating to and fusing with an oocyte is critical for sexual reproduction. As a major part of differentiation, sperm undergo extensive post-meiotic maturation en route to the oocyte. This is regulated largely by soma-derived cues. In C. elegans , this process is called sperm activation, and it transforms immotile spermatids into migratory, fertilization-competent cells. Here we show that the negative regulator of sperm activation, SWM-1, is produced in an unexpected cell type: body wall muscle...
November 23, 2018: Development
Jorge Merlet, Karinna Rubio-Peña, Sara Al Rawi, Vincent Galy
The nematode C. elegans represents a powerful experimental system with key properties and advantages to study the mechanisms underlying mitochondrial DNA maternal inheritance and paternal components sorting. First, the transmission is uniparental and maternal as in many animal species; second, at fertilization sperm cells contain both mitochondria and mtDNA; and third, the worm allows powerful genetics and cell biology approaches to characterize the mechanisms underlying the uniparental and maternal transmission of mtDNA...
November 23, 2018: Advances in Anatomy, Embryology, and Cell Biology
Yu Wen Ke, Jiang Liu
The chromatin accessibility and 3D high-order structure play an important role in gene expression and regulation, and are involved in numerous cellular processes such as differentiation, development and tumorigenesis. It is currently one of the hottest research fields in epigenetics. The animal embryonic development initiates from the terminally differentiated mature eggs, which are fertilized by sperms to establish totipotent zygotes. During this complex process, the epigenome encompassing chromatin accessibility and high-order structures changes dramatically, undergoes inheritance, reprogramming and re-establishment, so that the zygote could eventually develop into a new multicellular and multi-tissue organism...
November 20, 2018: Yi Chuan, Hereditas
Mahnaz Heidari, Sara Darbani, Mahsa Darbandi, Niknam Lakpour, Zohreh Fathi, Amir Hasan Zarnani, Hodjat Zeraati, Mohammad Mehdi Akhondi, Mohammad Reza Sadeghi
Selection of the best sperm, with the least defects, is a critical factor in the success of ART especially in male factor infertility. This study assessed the potential Heat shock protein (HSPA2) and metallopeptidase domain2 (ADAM2) biomarkers for sperm selection. Sperm were obtained from 72 asthenoteratozoospermic and 42 normospermic ejaculates. The semen characteristic, DNA fragmentation (DFI), chromatin maturation index (CMI), ADAM2 and HSPA2 levels on sperm, and their correlation with embryo quality were assessed in both groups...
November 22, 2018: Human Fertility: Journal of the British Fertility Society
Seyed-Mohammadreza Samaee, Nafiseh Manteghi, Alicia Estévez
Sperm quality is an important topic in general health, chemotherapy, and gamete preservation technology. Fatty acid (FA) composition of membranes, which is influenced by the diet, plays key roles in sperm biology and quality. Dietary supplementation with natural products can be used as a technique to screen potential agents to protect, modify, and recover sperm quality. In this study, zebrafish (male [♂-ZF] and female [♀-ZF]) were fed a single cultivar olive oil (OO) bioencapsulated in Artemia. OO-treated ♂-ZF had higher (p < 0...
November 20, 2018: Zebrafish
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