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https://www.readbyqxmd.com/read/28526792/environmental-spread-of-ndm-1-producing-multi-drug-resistant-bacteria-in-dhaka-bangladesh
#1
Mohammad Aminul Islam, Moydul Islam, Rashedul Hasan, M Iqbal Hossain, Ashikun Nabi, Mahdia Rahman, Wil H F Goessens, Hubert P Endtz, Alexandria B Boehm, Shah M Faruque
Resistance to carbapenem antibiotics through the production of New Delhi Metallo-β-lactamase-1 (NDM-1) constitutes an emerging challenge in the treatment of bacterial infections. To monitor possible source of spread of these organisms in Dhaka, Bangladesh, we conducted comparative analysis of waste water samples from hospital adjacent areas (HAR) and from community areas (COM) as well as public tap water samples for the occurrence and characteristics of NDM-1 producing bacteria. Of 72 HAR samples tested 51 (71%) were positive for NDM-1-producing bacteria as evidenced by phenotypic tests and the presence of blaNDM-1 gene, compared to 5 of 41 (12...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28515215/an-outbreak-of-ndm-1-producing-klebsiella-pneumoniae-in-a-dutch-hospital-with-interspecies-transfer-of-the-resistance-plasmid-and-unexpected-occurrence-in-unrelated-healthcare-centers
#2
Thijs Bosch, Suzanne P M Lutgens, Mirjam H A Hermans, Peter C Wever, Peter M Schneeberger, Nicole H M Renders, Alexander C A P Leenders, Jan A J W Kluytmans, Annelot Schoffelen, Daan Notermans, Sandra Witteveen, Erik Bathoorn, Leo M Schouls
Introduction. In the Netherlands, the number of cases with New Delhi metallo-beta-lactamase (NDM)-positive Enterobacteriaceae is low. Here, we report an outbreak of NDM-1 producing Klebsiella pneumoniae in a Dutch hospital with interspecies transfer of the resistance plasmid and unexpected occurrence in other unrelated healthcare centers (HCCs).Methods. Next-generation sequencing was performed on 250 carbapenemase-producing Enterobacteriaceae isolates including 42 NDM-positive isolates obtained from 29 persons at the outbreak site...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28512093/population-genomic-analysis-of-1-777-extended-spectrum-beta-lactamase-producing-klebsiella-pneumoniae-isolates-houston-texas-unexpected-abundance-of-clonal-group-307
#3
S Wesley Long, Randall J Olsen, Todd N Eagar, Stephen B Beres, Picheng Zhao, James J Davis, Thomas Brettin, Fangfang Xia, James M Musser
Klebsiella pneumoniae is a major human pathogen responsible for high morbidity and mortality rates. The emergence and spread of strains resistant to multiple antimicrobial agents and documented large nosocomial outbreaks are especially concerning. To develop new therapeutic strategies for K. pneumoniae, it is imperative to understand the population genomic structure of strains causing human infections. To address this knowledge gap, we sequenced the genomes of 1,777 extended-spectrum beta-lactamase-producing K...
May 16, 2017: MBio
https://www.readbyqxmd.com/read/28505330/acinetobacter-baumannii-transfers-the-blandm-1-gene-via-outer-membrane-vesicles
#4
Somdatta Chatterjee, Ayan Mondal, Shravani Mitra, Sulagna Basu
Objectives: To investigate the transmission of the gene encoding New Delhi metallo-β-lactamase-1 ( bla NDM-1 ) through outer membrane vesicles (OMVs) released from an Acinetobacter baumannii strain (A_115). Methods: Isolation and purification of OMVs by density gradient from a carbapenem-resistant clinical strain of A. baumannii harbouring plasmid-mediated bla NDM-1 and aac(6')-Ib-cr genes was performed. DNA was purified from the OMVs and used for PCR and dot-blot analysis...
May 12, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28490008/an-uncommon-st1224-ndm-1-producing-klebsiella-pneumoniae-isolated-from-the-bloodstream-of-a-leukemia-patient-in-china
#5
Xiaoxiao Zhang, Jia Du, Cui Zhou, Jianming Cao, Hong Lu, Haiyang Liu, Wenzi Bi, Tieli Zhou
AIMS: This study aimed to analyze the clinical data and characteristics of an NDM-1 (New Delhi metallo-β-lactamase-1)-producing Klebsiella pneumoniae isolated from the bloodstream of a leukemia patient. MATERIALS AND METHODS: A retrospective analysis was used for the clinical data of the patient. The modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA)-disk synergy test were used for detecting metallo-β-lactamase. Antibiotic resistance was determined using the agar dilution method...
May 11, 2017: Chemotherapy
https://www.readbyqxmd.com/read/28489087/hydrolysis-of-cephalexin-and-meropenem-by-new-delhi-metallo-%C3%AE-lactamase-the-substrate-protonation-mechanism-is-drug-dependent
#6
Chandan Kumar Das, Nisanth N Nair
Emergence of antibiotic resistance due to New Delhi metallo-β-lactamase (NDM-1) bacterial enzymes is of great concern due to their ability to hydrolyze a wide range of antibiotics. There are ongoing efforts to obtain the atomistic details of the hydrolysis mechanism in order to develop inhibitors for NDM-1. In particular, it remains elusive how drug molecules of different families of antibiotics are hydrolyzed by NDM-1 in an efficient manner. Here we report the detailed molecular mechanism of NDM-1 catalyzed hydrolysis of cephalexin, a cephalosporin family drug, and meropenem, a carbapenem family drug...
May 10, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28487071/diversity-oriented-sustainable-synthesis-of-antimicrobial-spiropyrrolidine-thiapyrrolizidine-oxindole-derivatives-new-ligands-for-a-metallo-%C3%AE-lactamase-from-klebsiella-pneumonia
#7
Anshu Dandia, Shahnawaz Khan, Pragya Soni, Aayushi Indora, Dinesh Kumar Mahawar, Prateek Pandya, Chetan Singh Chauhan
A simple, environmentally benign and highly proficient microwave assisted one-pot approach for the synthesis of antimicrobial spiropyrrolidine/thiapyrrolizidine oxindole derivatives is reported assembling two pharmacophoric moieties (1,3-indanedione and pyrrolidine/thiapyrrolizidine) in a single molecular framework via three-component 1,3-dipolar cycloaddition reaction of substituted isatin, sarcosine/1,3-thiazoles-4-carboxylic acid and Knoevenagel adduct (2-Cyano-3-phenyl-acrylic acid ethyl ester or 2-Benzylidene-malononitrile) in 2,2,2-trifluoroethanol as a reusable green solvent...
April 27, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28473912/high-burden-of-carbapenem-resistant-enterobacteriaceae-cre-fecal-carriage-at-a-teaching-hospital-cost-effectiveness-of-screening-in-low-resource-setting
#8
Abdul Rahman Zaidah, Nurul Izzah Mohammad, Siti Suraiya, Azian Harun
BACKGROUND: Infections by multidrug-resistant gram-negative bacteria (MDR-GNB) have been continuously growing and pose challenge to health institution globally. Carbapenem-resistant Enterobacteriacea (CRE) was identified as one of the MDR-GNB which has limited treatment options and higher mortality compared to those of sensitive strains. We report an increased burden of CRE fecal carriage at a hospital in the North-eastern region of Malaysia. METHODS: A retrospective descriptive study from August 2013 to December 2015 was conducted in the Medical Microbiology & Parasitology laboratory of Hospital Universiti Sains Malaysia, which is a tertiary teaching hospital with more than 700 beds...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28456489/evaluation-of-commercial-phenotypic-assays-for-the-detection-of-imp-or-new-delhi-metallo-%C3%AE-lactamase-producing-enterobacteriaceae-isolates-in-japan
#9
Daisuke Sakanashi, Makoto Kawachi, Yuki Uozumi, Mitsuru Nishio, Yuki Hara, Hiroyuki Suematsu, Mao Hagihara, Naoya Nishiyama, Nobuhiro Asai, Yusuke Koizumi, Yuka Yamagishi, Hiroshige Mikamo
OBJECTIVES: This study was designed to evaluate the sodium mercaptoacetic acid double disk synergy test (SMA-DDST), the Etest metallo-β-lactamase (MBL) MP/MPI (Etest MP/MPI), and the Mastdiscs ID Carbapenemase Detection Disc Set (MAST-CDS) for the detection of MBL-producing Enterobacteriaceae isolates in Japan. METHODS: Fifty-one clinical isolates and four reference strains were tested. These isolates included 40, 4, and 11 IMP-, New Delhi MBL (NDM)-, and non-MBL-producers, respectively...
April 26, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28449738/indistinguishable-ndm-producing-escherichia-coli-isolated-from-recreational-waters-sewage-and-a-clinical-specimen-in-ireland-2016-to-2017
#10
Bláthnaid M Mahon, Carina Brehony, Elaine McGrath, James Killeen, Martin Cormican, Paul Hickey, Shane Keane, Belinda Hanahoe, Ann Dolan, Dearbháile Morris
In this study, New Delhi metallo-beta-lactamase (NDM)-producing Enterobacteriaceae were identified in Irish recreational waters and sewage. Indistinguishable NDM-producing Escherichia coli by pulsed-field gel electrophoresis were isolated from sewage, a fresh water stream and a human source. NDM-producing Klebsiella pneumoniae isolated from sewage and seawater in the same area were closely related to each other and to a human isolate. This raises concerns regarding the potential for sewage discharges to contribute to the spread of carbapenemase-producing Enterobacteriaceae...
April 13, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28449650/structure-genetics-and-worldwide-spread-of-new-delhi-metallo-%C3%AE-lactamase-ndm-a-threat-to-public-health
#11
REVIEW
Asad U Khan, Lubna Maryam, Raffaele Zarrilli
BACKGROUND: The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. MAIN BODY: In this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species...
April 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28445009/evaluation-of-six-phenotypic-methods-for-the-detection-of-carbapenemases-in-gram-negative-bacteria-with-characterized-resistance-mechanisms
#12
Kunling Sun, Xiuyu Xu, Jinrong Yan, Liping Zhang
BACKGROUND: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. METHODS: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28432959/molecular-dynamics-simulation-studies-of-novel-%C3%AE-lactamase-inhibitor
#13
Farhan Ul Haq, Asma Abro, Saad Raza, Klaus R Liedl, Syed Sikander Azam
New Delhi Metallo-β-Lactamase-1 (NDM-1) has drawn great attention due to its diverse antibiotic resistant activity. It can hydrolyze almost all clinically available β-lactam antibiotics. To inhibit the activity of NDM-1 a new strategy is proposed using computational methods. Molecular dynamics (MD) simulations are used to analyze the molecular interactions between selected inhibitor candidates and NDM-1 structure. The enzyme-ligand complex is subject to binding free energy calculations using MM(PB/GB)SA methods...
April 4, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28417918/high-prevalence-of-blandm-1-blavim-qace-and-qace%C3%AE-1-genes-and-their-association-with-decreased-susceptibility-to-antibiotics-and-common-hospital-biocides-in-clinical-isolates-of-acinetobacter-baumannii
#14
Fatma Alzahraa M Gomaa, Zeinab H Helal, Mazhar I Khan
The objective of this study was to evaluate the susceptibility of metallo-β-lactamase (MBL)-producing Acinetobacter baumannii (A. baumannii) clinical isolates to biocides. We also determined the prevalence and correlation of efflux pump genes, class 1 integron and MBL encoding genes. In addition, blaVIM, blaNDM-1, qacE and qacEΔ1 nucleotide sequence analysis was performed and compared to sequences retrieved from GenBank at the National Center for Biotechnology Information database. A. baumannii had a resistance rate to carbapenem of 71...
April 12, 2017: Microorganisms
https://www.readbyqxmd.com/read/28416558/in-vitro-discordance-with-in-vivo-activity-humanized-exposures-of-ceftazidime-avibactam-aztreonam-and-tigecycline-alone-and-in-combination-against-new-delhi-metallo-%C3%AE-lactamase-producing-klebsiella-pneumoniae-in-a-murine-lung-infection-model
#15
M L Monogue, L M Abbo, R Rosa, J F Camargo, O Martinez, R A Bonomo, D P Nicolau
The management of infections with New Delhi Metallo-beta-lactamase-1 (NDM) producing bacteria remains clinically challenging given the multi-drug resistant (MDR) phenotype associated with these bacteria. Despite resistance in vitro, ceftazidime-avibactam previously demonstrated in vivo activity against NDM+ Enterobacteriaceae. Herein, we observed in vitro synergy with ceftazidime-avibactam and aztreonam against a MDR K. pneumoniae harboring NDM. In vivo, humanized doses of ceftazidime-avibactam monotherapy resulted in > 2 log10CFU bacterial reduction, therefore, no in vivo synergy was observed...
April 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28386246/virulence-and-genomic-feature-of-a-virulent-klebsiella-pneumoniae-sequence-type-14-strain-of-serotype-k2-harboring-blandm-5-in-china
#16
Yan-Fang Mei, Pan-Pan Liu, La-Gen Wan, Yang Liu, Lian-Hui Wang, Dan-Dan Wei, Qiong Deng, Xian-Wei Cao
The objective of this study was to reveal the molecular mechanism involved in carbapenem resistance and virulence of a K2 Klebsiella pneumoniae clinical isolate 24835. The virulence of the strain was determined by in vitro and in vivo methods. The de novo whole-genome sequencing technology and molecular biology methods were used to analyze the genomic features associated with the carbapenem resistance and virulence of K. pneumoniae 24835. Strain 24835 was highly resistant to carbapenems and belonged to ST14, exhibited hypermucoviscous and unique K2-aerobactin-kfu-rmpA positive phenotype...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28357923/performance-of-carbanp-and-cim-tests-in-oxa-48-carbapenemase-producing-enterobacteriaceae
#17
Serap Süzük Yıldız, Banu Kaşkatepe, Havva Avcıküçük, Şükran Öztürk
This study applied two phenotypic tests, namely "Carbapenemase Nordmann-Poirel" (CarbaNP) test and "Carbapenem Inactivation Method" (CIM), against the isolates carrying the carbapenem resistance genes. The study included 83 carbapenem-resistant Enterobacteriaceae isolates producing oxacillinase-48 (OXA-48) and 30 carbapenem-sensitive Enterobacteriaceae isolates. Out of the total isolates studied, 77 isolates (92.77%) were identified as Klebsiella pneumoniae and six isolates (7.23%) were identified as Escherichia coli by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry...
March 1, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28345184/spatial-molecular-epidemiology-of-carbapenem-resistant-and-new-delhi-metallo-beta-lactamase-blandm-producing-escherichia-coli-in-the-piglets-of-organized-farms-in-india
#18
B S Pruthvishree, O R Vinodh Kumar, D K Sinha, Y P S Malik, Z B Dubal, P A Desingu, M Shivakumar, N Krishnaswamy, B R Singh
AIM: A cross-sectional study was conducted in 10 government-organized pig farms between 2014 and 2016 representing seven states of India to understand the epidemiology of carbapenem resistance in the Escherichia coli. METHODS AND RESULTS: In this study, fecal sample (n = 673) from non-diarrheic (n = 501) and diarrheic (n = 172) piglets were processed for isolation of carbapenem resistant E. coli. Of 673, E. coli isolate (n = 112) was genotyped for confirming the carbapenem resistance and associated virulence factors...
March 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28337042/household-transmission-of-ndm-producing-e-coli-in-new-zealand
#19
Matthew Blakiston, Sally A Roberts, Joshua T Freeman, Helen Heffernan
This report describes the introduction of an extensively antibiotic-resistant carbapenemase-producing Escherichia coli into a hospital in Auckland, New Zealand, by a patient who was a household contact of recent travellers to the Indian subcontinent. The carbapenemase was identified as New Delhi metallo-β-lactamase (NDM) and reflects probable household transmission in the context of a recent upsurge in NDM-producing Enterobacteriaceae isolation in New Zealand. The observations in this report suggest that hospital screening practices to identify carbapenemase-producing Enterobacteriaceae (CPE) colonised patients may need to be extended to include travellers to high-risk countries who were not hospitalised during their trip, and possibly also their close contacts...
March 24, 2017: New Zealand Medical Journal
https://www.readbyqxmd.com/read/28319394/a-network-of-conformational-transitions-in-the-apo-form-of-ndm-1-enzyme-revealed-by-md-simulation-and-a-markov-state-model
#20
Kaifu Gao, Yunjie Zhao
New Delhi metallo-β-lactamase-1 (NDM-1) is a novel β-lactamase enzyme that confers enteric bacteria with nearly complete resistance to all β-lactam antibiotics, so it raises a formidable and global threat to human health. However, the binding mechanism between apo-NDM-1 and antibiotics as well as related conformational changes remains poorly understood, which largely hinders the overcoming of its antibiotic resistance. In our study, long-time conventional molecular dynamics simulation and Markov state models were applied to reveal both the dynamical and conformational landscape of apo-NDM-1: the MD simulation demonstrates that loop L3, which is responsible for antibiotic binding, is the most flexible and undergoes dramatic conformational changes; moreover, the Markov state model built from the simulation maps four metastable states including open, semiopen, and closed conformations of loop L3 as well as frequent transitions between the states...
March 30, 2017: Journal of Physical Chemistry. B
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