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Platelet cell indices

Guangjie Zhao, Wanling Wu, Xiaoqin Wang, Jingwen Gu
Acute megakaryocytic leukemia (AMKL) is a rare subtype of acute myeloid leukemia (AML), which is challenging to diagnose due to frequent myelofibrosis (MF) and a low percentage of blast cells. In the present study, clinical characteristics and experimental observations in 9 adult patients diagnosed with AMKL, who were recruited by the Sino-U.S. Shanghai Leukemia Co-operative Group, were analyzed in order to summarize the diagnostic experience and provide recommendations on diagnosing AMKL. All the patients were diagnosed according to the 2008 World Health Organization diagnostic criteria...
December 2018: Oncology Letters
Yongxu Mu, Xiaoyun Wu, Zhiming Hao
BACKGROUND: Within the past years, umbilical cord (UC) and amniotic membrane (AM) expanded in human platelet lysate (PL) have been found to become increasingly candidate of mesenchymal stromal cells (MSCs) in preclinical and clinical studies. Different sources of MSCs have different properties, and lead to different therapeutic applications. However, the similarity and differences between the AMMSCs and UCMSCs in PL remain unclear. RESULTS: In this study, we conduct a direct head-to-head comparison with regard to biological characteristics (morphology, immunophenotype, self-renewal capacity, and trilineage differentiation potential) and immunosuppression effects of AMMSCs and UCMSCs expanded in PL...
December 13, 2018: BMC Cell Biology
Christopher C Nguyen, Jeremy P Kamil
The past few years have brought substantial progress toward understanding how human cytomegalovirus (HCMV) enters the remarkably wide spectrum of cell types and tissues that it infects. Neuropilin-2 and platelet-derived growth factor receptor alpha (PDGFRα) were identified as receptors, respectively, for the trimeric and pentameric glycoprotein H/glycoprotein L (gH/gL) complexes that in large part govern HCMV cell tropism, while CD90 and CD147 were also found to play roles during entry. X-ray crystal structures for the proximal viral fusogen, glycoprotein B (gB), and for the pentameric gH/gL complex (pentamer) have been solved...
December 11, 2018: Viruses
Igor Giarretta, Ilaria Gatto, Margherita Marcantoni, Giulia Lupi, Diego Tonello, Eleonora Gaetani, Dario Pitocco, Roberto Iezzi, Addolorata Truma, Angelo Porfidia, Adriana Visonà, Paolo Tondi, Roberto Pola
Sonic hedgehog (Shh) is a prototypical angiogenic agent with a crucial role in the regulation of angiogenesis. Experimental studies have shown that Shh is upregulated in response to ischemia. Also, Shh may be found on the surface of circulating microparticles (MPs) and MPs bearing Shh (Shh + MPs) have shown the ability to contribute to reparative neovascularization after ischemic injury in mice. The goal of this study was to test the hypothesis that, in humans with peripheral artery disease (PAD), there is increased number of circulating Shh + MPs...
December 9, 2018: International Journal of Molecular Sciences
Jiang Qiu, Wang Lingn, Hu Jinghong, Zhang Yongqing
ETHNOPHARMACOLOGICAL RELEVANCE: Increased platelet aggregation plays a significant role in the aetiology of cardiovascular diseases (CVDs), which is the leading cause of death worldwide. Anti-platelet aggregation drugs such as aspirin, clopidogrel and prasugrel have been widely used in clinical, but some adverse reactions limit the clinical application. Leech, as an important Chinese medicine, is reported to prevent CVDs by multiple effects through oral administration, one of which is the significant inhibition of platelet aggregation, and its ability to do this has been extensively investigated in vivo and in clinical practice...
December 10, 2018: Journal of Ethnopharmacology
Marceau Quatredeniers, Morad Kamel Nakhleh, Sébastien Joël Dumas, Audrey Courboulin, Maria Candida Vinhas, Fabrice Antigny, Carole Phan, Christophe Guignabert, Imane Bendifallah, Matthieu Vocelle, Elie Fadel, Peter Dorfmüller, Marc Humbert, Sylvia Cohen-Kaminsky
In this study, we explored the complex interactions between platelet-derived growth factor (PDGF) and N-methyl D-aspartate receptor (NMDAR), and their effect on the excessive proliferation and migration of smooth muscle cells leading to obstructed arteries in Pulmonary Arterial Hypertension (PAH). We report lower expression of GluN2B, a subunit composing NMDARs expected to affect cell survival/proliferation of pulmonary artery smooth muscle cells (PASMCs), in PAH patients' lungs. PASMCs exposure to PDGF-BB, stimulated immediate increased levels of phosphorylated Src family kinases (SFKs) together with increased phosphorylated GluN2B (its active form) and cell surface relocalization, suggesting a cross-talk between PDGFR-recruited SFKs and NMDAR...
December 13, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
Fanjiu Kong, Yingbo Li, Xiaoxin Liu
Influence and clinical value of coagulation test on adverse reactions of blood transfusion in patients with major bleeding of ectopic pregnancy were analyzed. Six hundred and twenty-seven cases of ectopic pregnancy admitted to Zibo First Hospital from July 2014 to March 2017 were retrospectively analyzed. The 369 patients who had adverse reactions after blood transfusion were selected as the experimental group. Another 258 patients without adverse reaction after blood transfusion were selected as the control group...
December 2018: Experimental and Therapeutic Medicine
Xin Chen, Wansheng Peng, Zhen Zhang, Qian Zhao, Yan Zhou, Li Chen, Jiahua Pan
OBJECTIVE: To evaluate the efficacy and safety of selective brain hypothermia (SBH) in the treatment of neonates with moderate or severe neonatal hypoxic-ischemic encephalopathy (HIE), and the effect of SBH treatment on serum levels of neuron-specific enolase (NSE) and central nervous specific protein S100. METHODS: A prospective randomized controlled trial was conducted. From January 2015 to June 2017, 42 children with moderate to severe HIE in the neonatal intensive care unit (NICU) of the First Affiliated Hospital of Bengbu Medical College were enrolled, and they were randomly divided into SBH treatment group and routine treatment group after obtaining the consent of the guardian of the children...
November 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Preeti Kumari Chaudhary, Soochong Kim
Platelet activation plays a major role in hemostasis and thrombosis. Various agonists including ADP and thrombin interact with GPCRs which transduce signals from the cell surface to intracellular effectors through various G proteins. Recent studies have elucidated the role of these receptors and their corresponding G proteins in the regulation of events involved in platelet activation. However, these agonists-induced platelet activation in companion animals is poorly understood. This study was designed to characterize the platelet response to various agonists in dog platelets...
December 4, 2018: Journal of Veterinary Science
Léanne Swart, Elise Schapkaitz, Johnny N Mahlangu
INTRODUCTION: Thrombotic thrombocytopenic purpura (TTP) is associated with high mortality if not managed timeously with therapeutic plasma exchange (TPE). TTP secondary to human immunodeficiency virus (HIV) infection is unique to sub-Saharan Africa. The management and outcome of TTP in the era of improved access to therapy has not been described. METHODS: The present study describes the clinical presentation, treatment, therapeutic endpoints, and outcome of TTP patients at the Charlotte Maxeke Johannesburg Academic Hospital, South Africa...
December 8, 2018: Journal of Clinical Apheresis
Ruifang Zhou, Haishan Long, Bei Zhang, Zhizhao Lao, Quanyu Zheng, Tiancheng Wang, Yongxin Zhang, Qingguang Wu, Xiaoping Lai, Geng Li, Lizhu Lin
Salvianolic acid B (SB) is an antioxidant derived from Salvia militarize, and is one of the most widely used herbs in traditional Chinese medicine. SB is a potent antioxidant that has been well documented as a scavenger of oxygen free radicals, and has been used for the prevention and treatment of atherosclerosis‑associated disorders. To explore its potential therapeutic effects in treating radiation damage, in this study, mice were treated with SB at different doses of 5, 12.5 and 20 mg/kg, subsequent to receiving γ‑irradiation...
December 3, 2018: Molecular Medicine Reports
José Juan-Colás, Ian S Hitchcock, Mark Coles, Steven Johnson, Thomas F Krauss
Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution...
December 10, 2018: Proceedings of the National Academy of Sciences of the United States of America
Marcel Orth, Takhirjan Shadmanov, Claudia Scheuer, Benedikt J Braun, Tobias Fritz, Jörg H Holstein, Tina Histing, Matthias W Laschke, Tim Pohlemann, Michael D Menger
Approximately 10% of all fractures result in delayed healing or non-unions. Bone healing can be improved by the application of osteoconductive and osteoinductive biomaterials. Microcalcite (MCA) as a naturally available calcium carbonate-based biomaterial derived from marble may have the potential to improve bone healing. Herein, we studied for the first time, if MCA in combination with platelet-rich plasma (PRP) can be used as a bone graft material for bone healing in vivo. For this purpose, osteotomies were induced in CD-1 mice (n = 60)...
December 7, 2018: Biomedical Materials
Zhimeng Lv, Ming Guo, Chenghua Li, Yina Shao, Xuelin Zhao, Weiwei Zhang
Vascular endothelial growth factor (VEGF) is a key conservative regulator of inflammation response by promoting cell proliferation, migration, and vascular permeability. It also induces the release of inflammatory factors in vertebrates. We previously characterized NLR family pyrin domain containing 3 and HMGB3 homology in Apostichopus japonicus, providing the occurrence of inflammation in this species. However, to our knowledge, other inflammation-related molecules, such as VEGF, have rarely been investigated...
December 2, 2018: Developmental and Comparative Immunology
Wen-Chao Wang, Xiao-Feng Zhang, Jian Peng, Xi-Feng Li, Ai-Li Wang, Ya-Qin Bie, Le-Hua Shi, Mou-Bin Lin, Xiao-Feng Zhang
Circulating tumor cells (CTCs) are cancer cells shed from either the primary tumor or its metastases that circulate in the peripheral blood. The CTCs are regarded as the source of tumor recurrence and metastasis and speculated as the indicators of residual tumors, thereby indicating a poor prognosis. Although CTCs play a vital role in tumor metastasis and recurrence, little is known about the underlying survival mechanisms in the blood circulation. The accumulating evidence has revealed that CTCs might survive in the peripheral blood by overcoming the mechanical damage due to shear stress, resistance to anoikis, evasion of immune destruction, and resistance to chemotherapy...
2018: BioMed Research International
Violet Kasabri, Esraa Shawakri, Amal Akour, Randa Naffa, Nahla Khawaja, Ibrahim Al-Sarraf, Jameel Bzour
Background: Oxytocin (OXT), fetuin-A and interleukin-18 (IL-18) are involved in the development and progression of metabolic syndrome (MetS) and prediabetes (pre/T2DM). Aims participants and methods: This study aimed to compare and correlate the plasma levels of OXT, fetuin-A and IL-18 with clinical parameters, haematological indices and adiposity indices in Jordanian MetS subjects. In a cross-sectional study, 30 normoglycaemic lean study participants (control), 30 MetS study participants, and 29 MetS pre/T2DM study participants were recruited...
December 2018: Therapeutic Advances in Endocrinology and Metabolism
Jan Rossaint, Andreas Margraf, Alexander Zarbock
Platelets are most often recognized for their crucial role in the control of acute hemorrhage. However, current research has greatly expanded the appreciation of platelets beyond their contribution to primary hemostasis, indicating that platelets also actively participate in leukocyte recruitment and the regulation of the host defense in response to exogenous pathogens and sterile injury. Early recruitment of leukocytes, especially neutrophils, is the evolutionary stronghold of the innate immune response to successfully control exogenous infections...
2018: Frontiers in Immunology
Khairul Syahputra, Per W Kania, Azmi Al-Jubury, Rzgar M Jafaar, Ron P Dirks, Kurt Buchmann
The parasite Ichthyophthirius multifiliis infecting skin, fins and gills of a wide range of freshwater fish species, including rainbow trout, is known to induce a protective immune response in the host. Although a number of studies have reported activation of several immune genes in infected fish host, the immune response picture is still considered incomplete. In order to address this issue, a comparative transcriptomic analysis was performed on infected versus uninfected rainbow trout gills and it showed that a total of 3352 (7...
December 1, 2018: Fish & Shellfish Immunology
Juan Martin-Liberal, Ezequiel Pérez, Xavier García Del Muro
Soft-tissue sarcomas (STS) are a heterogeneous group of diseases that are characterized by a historic lack of active treatment options. However, several new drugs and indications have become available in recent years. Areas covered: This article reviews the most relevant phase II studies that utilize chemotherapy agents (aldoxorubicin, amrubicin, trabectedin alone or in combination with doxorubicin, and gemcitabine plus docetaxel), targeted therapies (Imatinib, dasatinib, regorafenib, tivozanib, palbociclib and selinexor), a combination of chemotherapy plus targeted therapies (fucusing on doxorubicin plus olaratumab) and immunotherapies (pembrolizumab, combination of nivolumab plus ipilimumab and adaptive cell therapy) in STS (other than gastrointestinal stromal tumors) (GIST) published from 2015...
December 4, 2018: Expert Opinion on Investigational Drugs
Mari Kawamura, Tadashi Yamamoto, Keisuke Yamashiro, Shinsuke Kochi, Chiaki Yoshihara-Hirata, Hidetaka Ideguchi, Hiroaki Aoyagi, Kazuhiro Omori, Shogo Takashiba
The recruitment of tissue-resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, co-ordinated by growth factors and the extracellular matrix (ECM). Integrin-mediated cell adhesion to the ECM provides essential signals for migration. We have been suggested that PDL cell migration could be enhanced by selective expression of integrins...
December 3, 2018: Journal of Cellular and Molecular Medicine
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