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Platelet cell indices

Xavier Charest-Morin, Jacques Hébert, Georges-Étienne Rivard, Arnaud Bonnefoy, Eric Wagner, François Marceau
Multiple pathways have been proposed to generate bradykinin (BK)-related peptides from blood. We applied various forms of activation to fresh blood obtained from 10 healthy subjects or 10 patients with hereditary angioedema (HAE-1 or -2 only) to investigate kinin formation. An enzyme immunoassay for BK was applied to extracts of citrated blood incubated at 37°C under gentle agitation for 0-2 h in the presence of activators and/or inhibitory agents. Biologically active kinins in extracts were corroborated by c-Fos accumulation in HEK 293a cells that express either recombinant human B2 or B1 receptors (B2 R, B1 R)...
2018: Frontiers in Immunology
Xiaoxuan Lei, Pengcheng Xu, Biao Cheng
BACKGROUND: In recent years, platelet-rich plasma (PRP) has been widely applied in orthopedics, maxillofacial surgery, burns, and plastic surgery, especially in facial rejuvenation. Research is ongoing into new indications and mechanisms of PRP to promote its wider, safer, and more effective use in the clinic. This article reviews the possible mechanisms of PRP in facial rejuvenation and related research. It is expected that the application of PRP in this field will increase. METHODS: The use of PRP in facial rejuvenation was screened using inclusion and exclusion criteria...
October 16, 2018: Aesthetic Plastic Surgery
Kazuhiro Araki, Yoshinori Ito, Ippei Fukada, Kokoro Kobayashi, Yoshimasa Miyagawa, Michiko Imamura, Ayako Kira, Yuichi Takatsuka, Chiyomi Egawa, Hirofumi Suwa, Shinji Ohno, Yasuo Miyoshi
BACKGROUND: Although peripheral blood-based parameters (PBBPs) are reported as prognostic indicators in patients with breast cancers, their utility has not been studied in human epidermal growth factor receptor 2 (HER2)-positive advanced breast cancer (ABC). Tumor-infiltrating lymphocytes (TILs) might be a predictive factor in patients with HER2-positive ABC treated with pertuzumab and trastuzumab (PT) plus docetaxel. We aimed to evaluate whether PBBPs could have predictive value in HER2-positive ABC treated with pertuzumab and trastuzumab (PT) combined with eribulin (ERI) or nab-paclitaxel (Nab-PTX)...
October 16, 2018: BMC Cancer
Christopher S Farrar, Denise C Hocking
Cellular responses to platelet-derived growth factor (PDGF) are altered in a variety of pathological conditions, including cancers, fibroses, and vascular diseases, making PDGF-induced signaling pathways important therapeutic targets. The limited success of therapies designed to impact PDGF pathways may be overcome with a clearer understanding of how cells integrate signals from PDGF and the extracellular matrix (ECM). Here, we assessed the effects of fibronectin matrix assembly on the responsiveness of mesenchymal cells to PDGF...
October 15, 2018: Journal of Biological Chemistry
Eunji Ko, Jung Min Youn, Hyung Sun Park, Myeongjin Song, Kyung Hee Koh, Choon Hak Lim
BACKGROUND: Sepsis is a medical emergency during which early detection is closely associated with mortality. In sepsis, red blood cell (RBC) abnormalities have been reported. However, it is not known how early RBC abnormalities are expressed compared with various clinical manifestations used in sepsis-related organ failure assessment (SOFA). OBJECTIVE: Therefore, using a lipopolysaccharide (LPS)-induced sepsis model we investigated the clinical significance of RBC abnormalities as an early indicator in the detection of septic injury compared with clinical variables...
October 4, 2018: Clinical Hemorheology and Microcirculation
Giuseppe Di Stolfo
BACKGROUND: Carotid stenting stimulates intimal proliferation through platelet and stem cell activation. OBJECTIVE: The aim of this study is to evaluate whether the administration before or after carotid stenting of clopidogrel loading dose may play a role on circulating endothelial progenitor cells, stromal cell-derived factor-1α (SDF-1α) and neointimal hyperplasia. METHODS: We recruited 13 patients (aged 74.52±7.23) with indication of carotid revascularization and in therapy with salicylic acid and statin...
September 29, 2019: Clinical Hemorheology and Microcirculation
Giovanni Giurdanella, Giuseppe Montalbano, Florinda Gennuso, Serena Brancati, Debora Lo Furno, Antonio Augello, Claudio Bucolo, Filippo Drago, Salvatore Salomone
The study of strial pericytes has gained great interest as they are pivotal for the physiology of stria vascularis. To provide an easily accessible in vitro model, here we described a growth medium-based approach to obtain and cultivate primary bovine cochlear pericytes (BCP) from the stria vascularis of explanted bovine cochleae. We obtained high-quality pericytes in 8-10 days with a > 90% purity after the second passage. Immunocytochemical analysis showed a homogeneous population of cells expressing typical pericyte markers, such as neural/glial antigen 2 (NG2), platelet-derived growth factor receptorβ (PDGFRβ), α-smooth muscle actin (α-SMA), and negative for the endothelial marker von Willebrand factor...
October 14, 2018: Journal of Cellular Physiology
Chris Hyunchul Jo, Seung Yeon Lee, Kang Sup Yoon, Sohee Oh, Sue Shin
BACKGROUND: Although platelet-rich plasma (PRP) is a popular option for rotator cuff disease, the underlying mechanism of PRP and its clinical indications are unclear. Further, some kinds of PRP might be detrimental to patients. Allogenic PRP prepared through a standardized process and fully characterized could eliminate variations in PRP as well as uncertainties regarding its use in each patient, which could provide clues about its mechanism of action and indications for its use. PURPOSE: To assess the effects of pure PRP on tenocytes with or without inflammation in an in vitro study and to evaluate the safety and efficacy of a fully characterized pure PRP injection in patients with rotator cuff disease in a clinical study...
October 12, 2018: American Journal of Sports Medicine
Zhigang Liu, Xiangxin Li, Meipin Zhang, Xiaofei Huang, Jun Bai, Zhiwei Pan, Xiuxian Lin, Dongni Yu, Huaping Zeng, Ruiping Wan, Xingguang Ye
Systemic inflammatory response has been implicated as a contributor to the onset of febrile seizures (FS). The four novel indices of the inflammatory response such as, neutrophil-to-lymphocyte ratio (NLR), mean platelet volume (MPV), platelet count (PLT) ratio and red blood cell distribution width (RDW) have been investigated in FS susceptibility and FS types (simple febrile seizure and complex febrile seizure). However, the potential role of these inflammatory markers and MPV/PLT ratio (MPR) in Chinese children with FS has yet to be fully determined...
October 11, 2018: Scientific Reports
Sanjay Khandelwal, Joann Ravi, Lubica Rauova, Alexandra Johnson, Grace M Lee, Jennifer B Gilner, Sreenivasulu Gunti, Abner L Notkins, Maragatha Kuchibhatla, Michael Frank, Mortimer Poncz, Douglas B Cines, Gowthami M Arepally
The mechanisms by which exposure to heparin initiates antibody responses in many, if not most, recipients are poorly understood. We recently demonstrated that antigenic platelet factor 4 (PF4)/heparin complexes activate complement in plasma and bind to B cells. Here, we describe how this process is initiated. We observed wide stable variation in complement activation when PF4/heparin was added to plasma of healthy donors, indicating a responder "phenotype" (high, intermediate or low). Proteomic analysis of plasma from these healthy donors showed a strong correlation between complement activation and plasma IgM levels (r = 0...
October 11, 2018: Blood
Takashi Ashida
"Guidelines for the use of red blood cell products, platelet transfusion concentrates and fresh frozen plasma based on scientific evidence" was published by the Japan Society of Transfusion Medicine and Cell Therapy. Each transfused blood product carries a certain risk of an acute or late adverse event. Hematologists must be familiar with transfusion-associated adverse events, such as transfusion-related acute lung injury, transfusion-associated circulatory overload, post-transfusion graft-versus-host disease, transfusion-transmitted viral infection, etc...
2018: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
Isa Sincer, Yilmaz Gunes, Asli Kurtar Mansiroglu, Mehmet Cosgun, Gulali Aktas
Introduction: The prognostic value of hematological indices in cardiovascular diseases and the association between these parameters and cardiovascular conditions have been established in the literature. Aim: In this study, we aimed to investigate the relation of mean platelet volume (MPV), MPV to platelet ratio (MPR) and red cell distribution width (RDW) with degree of coronary collateral development (CCD) in stable coronary artery disease (CAD) subjects with established critical coronary artery stenosis...
2018: Postępy W Kardiologii Interwencyjnej, Advances in Interventional Cardiology
Natalia I Ossetrova, Paul Stanton, Katya Krasnopolsky, Mohammed Ismail, Arpitha Doreswamy, Kevin P Hieber
The risk of potential radiation exposure scenarios that include detonation of nuclear weapons, terrorist attacks on nuclear reactors, and the use of conventional explosives to disperse radioactive substances has increased in recent years. The majority of radiation biodosimetry and countermeasure studies have been performed using photon radiation even though many exposure scenarios predict mixed-field (neutron and photon) radiation. Hence, there is a need to evaluate biomarkers and accurately determine exposure levels of mixed-field combinations of neutrons and photons for an individual...
October 5, 2018: Health Physics
Vlad M Anghelescu, Ioana Neculae, Octavian Dincă, Cristian Vlădan, Claudiu Socoliuc, Mirela Cioplea, Luciana Nichita, Cristiana Popp, Sabina Zurac, Alexandru Bucur
Introduction: The clinical use of bioactive materials for bone augmentation has remained a challenge because of predictability and effectiveness concerns, as well as increased costs. The purpose of this study was to analyse the ability to integrate bone substitutes by evaluating the immunohistochemical expression of the platelet endothelial cell adhesion molecules, vascular endothelial growth factor, collagen IV, laminin, and osteonectin, in the vicinity of bone grafts, enabling tissue revascularization and appearance of bone lamellae...
2018: Journal of Immunology Research
Yaqiong Tang, Xinyou Zhang, Shiyu Han, Tiantian Chu, Jiaqian Qi, Hong Wang, Xiaowen Tang, Huiying Qiu, Chengcheng Fu, Changgeng Ruan, Depei Wu, Yue Han
Severe thrombocytopenia is a serious condition that frequently arises in patients with myelodysplastic syndrome (MDS) and is associated with poor prognosis. Few studies have investigated the prognostic significance of platelet recovery in patients with MDS having thrombocytopenia. We retrospectively analyzed 117 patients with de novo MDS complicated with thrombocytopenia (platelet count [PLT] < 100 × 109 /L). Patients received decitabine treatment (schedule A) or decitabine followed by allogeneic hematopoietic stem cell transplantation (allo-HSCT; schedule B)...
October 8, 2018: Clinical and Applied Thrombosis/hemostasis
Naomi Martin, Alice C Smith, Maurice R Dungey, Hannah M L Young, James O Burton, Nicolette C Bishop
Hemodialysis patients have dysfunctional immune systems, chronic inflammation and comorbidity-associated risks of cardiovascular disease (CVD) and infection. Microparticles are biologically active nanovesicles shed from activated endothelial cells, immune cells, and platelets; they are elevated in hemodialysis patients and are associated with chronic inflammation and predictive of CVD mortality in this group. Exercise is advocated in hemodialysis to improve cardiovascular health yet acute exercise induces an increase in circulating microparticles in healthy populations...
September 2018: Physiological Reports
Giovanni Cimmino, Roberta Tarallo, Stefano Conte, Andrea Morello, Grazia Pellegrino, Francesco S Loffredo, Gaetano Calì, Nicola De Luca, Paolo Golino, Bruno Trimarco, Plinio Cirillo
INTRODUCTION: Platelets activation/aggregation with subsequent thrombus formation is the main event in the pathophysiology of acute coronary syndrome. Once activated, platelets show an extensive cytoskeleton rearrangement that leads to recruitment of additional platelets to finally cause haemostatic plug formation. Thus, the cytoskeleton plays a pivotal role in this phenomenon. Colchicine (COLC) is an anti-inflammatory drug proven to reduce major cardiovascular events in patients with coronary artery disease...
October 1, 2018: Vascular Pharmacology
Jessica Morales-Ortíz, Victoria Deal, Fiorella Reyes, Gerónimo Maldonado-Martínez, Nahomy Ledesma, Franklin Staback, Cheyanne Croft, Amanda Pacheco, Humberto Ortiz-Zuazaga, C Christian Yost, Jesse W Rowley, Bismark Madera, Alex St John, Junmei Chen, Jose Lopez, Matthew T Rondina, Robert Hunter, Angela Gibson, A Valance Washington
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) affect over 200,000 individuals yearly with a 40% mortality rate. While platelets are implicated in the progression of ALI/ARDS, their exact role remains undefined. Triggering Receptor Expressed in Myeloid cells (TREM)-Like Transcript (TLT)-1 is found on platelets, binds fibrinogen, and mediates clot formation. We hypothesized that platelets use TLT-1 to manage the progression of ALI/ARDS. Here we retrospectively measure plasma levels of sTLT-1 from the ARDSNET clinical trial and show that patients whose sTLT-1 levels were >1200pg/mL had nearly twice the mortality risk as those with <1200 pg/mL (p<0...
October 3, 2018: Blood
Takao Ukaji, Ai Takemoto, Ryohei Katayama, Kengo Takeuchi, Naoya Fujita
Hematogenous metastases are enhanced by platelet aggregation induced by tumor cell-platelet interaction. Podoplanin is a key molecule to enhance the platelet aggregation and interacts with C-type lectin-like receptor 2 (CLEC-2) on platelet via PLAG domains. Our previous reports have shown that blocking podoplanin binding to platelets by neutralizing antibody specific to PLAG4 domain strongly reduces hematogenous metastasis. However, podoplanin is expressed in a variety of normal tissues such as lymphatic vessels and the question remains whether treatment of tumors with anti-podoplanin neutralizing antibodies would be toxic...
September 7, 2018: Oncotarget
Masafumi Kusunoki, Eri Sata, Kensuke Nishio, Takayoshi Tanaka, Tetsuya Nishida, Naoyuki Sugano, Shuichi Sato, Masatake Asano
Extracellular matrix metalloproteinase inducer (EMMPRIN) secretion was induced in the oral squamous cell carcinoma cell line HSC3 cell by acid-electrolyzed functional water (FW) stimulation. Augmented EMMPRIN secretion was not under transcriptional control; rather, it was derived from the intracellular storages. EMMPRIN secretion was also induced under oxidative stress and accompanied by the release of lactate dehydrogenase (LDH). The molecules released from cells undergoing necrosis are called as alarmins, and the secretion of IL-1α, a typical alarmin, was induced by FW stimulation and oxidative stress...
2018: International Journal of Medical Sciences
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