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Kun Li, Chunmei Xiu, Qiang Zhou, Li Ni, Jun Du, Tingting Gong, Meng Li, Saijilafu, Huilin Yang, Jianquan Chen
Osteoblasts, the chief bone-forming cells, are differentiated from mesenchymal stromal/stem cells. Disruption of this differentiation process can cause osteoporosis, a bone disease characterized by low bone mass and deteriorated bone structure. Cholesterol has been implicated in pathogenesis of osteoporosis, and was recently identified as an endogenous activator of Hedgehog (Hh) signaling. However, its pathological and physiological roles in osteoblast differentiation are still poorly understood. Moreover, it is unclear whether these potential roles played by cholesterol are related to its capability to modulate Hh pathway...
October 14, 2018: Journal of Cellular Physiology
Fashui Hong, Yingjun Zhou, Lingqun Ye, Yuguan Ze, Jianhui Ji, Juan Zhuang, Ling Wang
Nanosized titanium dioxide (Nano TiO₂) has been widely used in daily lives, medicine, industry, and caused the potential reproduction toxicity for animals and human, however, the underlying molecular mechanisms for the reproductive toxicity of nano TiO₂ are still largely unclear. In the present study, when primary cultured rat Sertoli cells (SCs) were exposed to nano TiO₂, cell injury and alterations in wingless related MMTV integration site (Wnt) pathway-related factors including Wnt1, Wnt3a, Wnt5a, Wnt11, β -catenin, and p-GSK-3 β expression were investigated...
December 1, 2018: Journal of Biomedical Nanotechnology
Mario Vallon, Kanako Yuki, Thi D Nguyen, Junlei Chang, Jenny Yuan, Dirk Siepe, Yi Miao, Markus Essler, Makoto Noda, K Christopher Garcia, Calvin J Kuo
WNT7A and WNT7B control CNS angiogenesis and blood-brain barrier formation by activating endothelial Wnt/β-catenin signaling. The GPI-anchored protein RECK and adhesion G protein-coupled receptor GPR124 critically regulate WNT7-specific signaling in concert with FZD and LRP co-receptors. Here, we demonstrate that primarily the GPR124 ectodomain, but not its transmembrane and intracellular domains, mediates RECK/WNT7-induced canonical Wnt signaling. Moreover, RECK is the predominant binding partner of GPR124 in rat brain blood vessels in situ...
October 9, 2018: Cell Reports
Mingzhi Shen, Danna Bai, Bei Liu, Xiaozhao Lu, Rongrong Hou, Chao Zeng, Na Li, Zhenhong Fu, Congye Li, Ling Tao, Haichang Wang, Tao Yin
Hyperglycemia-induced impairment of angiogenesis contributes to the unfavorable prognosis of myocardial ischemia in long-standing diabetes mellitus. The underlying mechanism remains largely unknown and therapeutic strategies thereby limited. In the present study, we investigated the possible involvement of thioredoxin-interacting protein (TXNIP) and Wnt/β-catenin signaling in the context, and their possible relation was also explored. STZ induced diabetic mice were subjected to myocardial infarction (MI). Adenovirus expressing shTXNIP, shCtnnb1 (β-catenin) driven by VE-Cadherin promoter was administered intramyocardially immediately after MI...
September 24, 2018: Biochimica et biophysica acta. Molecular basis of disease
Sheng-Qun Liu, Jing-Liang Zhang, Zhan-Wen Li, Zhen-Hua Hu, Zhe Liu, Yi Li
BACKGROUND: Propofol is a commonly used anaesthetic with controversial effects on cancer cells. We aimed to explore the functional roles of propofol in hepatocellular carcinoma (HCC) cells as well as the underlying mechanisms. METHODS: HepG2 and SMMC-7721 cells were used in this study. Firstly, the effects of propofol on cell viability, migration, invasion, apoptosis, and involved proteins were assessed by Cell Counting Kit-8 assay, Transwell assay, flow cytometry assay and Western blot analysis, respectively...
2018: Cellular Physiology and Biochemistry
Cameron C Scott, Stefania Vossio, Jacques Rougemont, Jean Gruenberg
How trafficking pathways and organelle abundance adapt in response to metabolic and physiological changes is still mysterious, although a few transcriptional regulators of organellar biogenesis have been identified in recent years. We previously found that the Wnt signaling directly controls lipid droplet formation, linking the cell storage capacity to the established functions of Wnt in development and differentiation. In the present paper, we report that Wnt-induced lipid droplet biogenesis does not depend on the canonical TCF/LEF transcription factors...
September 26, 2018: ELife
Hongying Wang, Yan Bai, Chengdong Liu, Jianfeng Zhou, Xiaozhi Rong
Ror family of receptor tyrosine kinases ROR1 and ROR2 plays crucial roles in animal development by regulating cell proliferation, differentiation, and migration, as well as survival and death by acting as a receptor or co-receptor for Wnt5a and mediating Wnt5a-induced activation. Compared with our extensive understanding of ROR2, our knowledge of ROR1 is limited. In this study, we characterized the zebrafish ror1 gene and determined its temporal and spatial expression and biological activity. Sequence comparison and phylogenetic analyses indicate that its protein structure is similar to its mammalian orthologs...
September 21, 2018: Fish Physiology and Biochemistry
Qiushi Li, Chen Li, Si Xi, Xianjing Li, Lina Ding, Meihua Li
Photobiomodulation therapy (PBMT) has been demonstrated as regulating osteoblast proliferation. MicroRNAs (miRNAs) are involved in various pathophysiologic processes in osteoblast, but the role of miRNAs in the PBMT-based promotion of osteoblast proliferation remains unclear. This study aimed to investigate the effects of PBMT treatment (3.75 J/cm2 ) on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via the miR-503/Wnt3a pathway; meanwhile, detect the expressions of miR-503 and Wnt3a after PBMT treatment and the role of miR-503 in regulating Wnt signaling molecules Wnt3a, β-catenin, Runx2, apoptotic proteins caspase-3, and Bcl-2 in vitro...
September 15, 2018: Lasers in Medical Science
Youpei Wang, Peipei Lin, Qing Wang, Meiqin Zheng, Lingxia Pang
The Wnt and Hedgehog signalling pathways serve key roles in diverse developmental processes. However, the molecular associations between these two signalling pathways remains unclear. Previous transcriptome studies on human foreskin fibroblasts have indicated that Wnt signalling activation induces the expression of key Hedgehog signalling genes, including smoothened, frizzled class receptor ( Smo ) and GLI family zinc finger 1 ( Gli1 ). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) results revealed that Wnt3a treatment induced the expression of the key Hedgehog signalling genes, including Smo , patched ( PTCH ), Gli1, Gli2 and Gli3 ...
September 2018: Experimental and Therapeutic Medicine
Mohamed M Elseweidy, Hoda E Mohamed, Rania A Elrashidy, Hebatallah H Atteia, Gehad M Elnagar, Abd El-Monem Ali
The present study aimed to investigate the inhibitory effects of 10-dehydrogingerdione (10-DHGD) and pentoxifylline (PTX) either individually or in combined form on calcium deposition in high cholesterol diet (HCD)-fed rabbits as compared to atorvastatin (ATOR), and to clarify the underlying mechanisms. Three-months-old male New Zealand white rabbits received either normal chow or HCD for 12 weeks. The latter group was subdivided into five groups and concurrently treated either with vehicle (dyslipidemic control), ATOR, 10-DHGD, PTX or combined 10-DHGD and PTX...
September 1, 2018: Molecular and Cellular Biochemistry
Fengfei Lu, Yongyi Ye, Hongbo Zhang, Xiongjun He, Xiang Sun, Chen Yao, Hengxu Mao, Xiaozheng He, Chen Qian, Baoyan Wang, Linghai Xie, Tao Xie, Guohui Lu, Shizhong Zhang
Glioma is one of the most frequent intracranial malignant tumors. Abnormal expression of microRNAs usually contributes to the development and progression of glioma. In the current study, we explored the role and underlying mechanism of miR-497 in glioma. We revealed that miR-497 expression was significantly down-regulated in glioma tissues and cell lines. Reduced expression of miR-497 was associated with poor disease-free and over-all survival rate. Restoration of miR-497 decreased glioma cell growth and invasion both in vitro and in vivo...
December 2018: International Journal of Biological Macromolecules
Hilda Merino, Dinender K Singla
Doxorubicin (Dox) is a potent chemotherapeutic drug known for its dose-dependent and serious adverse effects, such as cardiotoxicity and myotoxicity. Dox-induced cardiotoxicity (DIC) and muscle toxicity (DIMT) have been studied; however, the mechanisms of Dox-induced apoptosis in soleus muscle are not well defined. Our data shows that with Dox treatment, there is a significant increase in oxidative stress, apoptosis, proapoptotic protein BAX, pPTEN levels, and wnt3a and β -catenin activity ( p < 0.05). Moreover, Dox treatment also resulted in decreased antioxidant levels, antiapoptotic BCL2, pAKT, p-mTOR, and endogenous levels of sFRP2 in the soleus muscle tissue ( p < 0...
2018: Oxidative Medicine and Cellular Longevity
Yu You, Keting Que, Yun Zhou, Zhen Zhang, Xiaoping Zhao, Jianpin Gong, Zuojin Liu
Recent studies have indicated that microRNAs (miRNAs) play an important role in hepatocellular carcinoma (HCC) progression. In this study, we showed that miR-766-3p was decreased in approximately 72% of HCC tissues and cell lines, and its low expression level was significantly correlated with tumour size, TNM stage, metastasis, and poor prognosis in HCC. Ectopic miR-766-3p expression inhibited HCC cell proliferation, colony formation, migration and invasion. In addition, we showed that miR-766-3p repressed Wnt3a expression...
August 27, 2018: Molecules and Cells
Yajun Luo, Wang Tan, Weikun Jia, Zilin Liu, Pengcheng Ye, Zhiming Fu, Fei Lu, Wanping Xiang, Linghan Tang, Lin Yao, Qingmei Huang, Jiangwei Xiao
The dysregulation of long non-coding RNA (lncRNA) has increasingly been linked to human gastric cancer (GC). However, the LINC01606 expression level and clinical values, and its role in the molecular mechanism underlying GC remain largely unknown. In our research, we found that LINC010606 was elevated aberrantly and correlated with metastasis and invasion in GC patients. Moreover, we found that LINC01606 expression level was associated with Wnt/β-catenin signaling. In addition, subsequent functional experiments showed that JW74, a specific Wnt/β-catenin signaling inhibitor, inhibited the transcription of LINC01606 and suppressed migration and invasion in GC cell lines...
October 2018: International Journal of Biochemistry & Cell Biology
Carolyn A Meyers, Jia Shen, Amy Lu, Aaron W James
Perivascular stem cells (PSC) are a progenitor population defined by their perivascular residence. Recent studies have examined the relative difference in Wnt ligands to induce PSC differentiation, including Wnt16. Here, we examine the role of Wnt16 in the proliferation and osteogenic differentiation of human PSC. Treatment of PSC with WNT16 significantly increased cell proliferation to a greater extent than did WNT3A. In addition, WNT16 showed a significant increase in osteogenic gene expression among PSC...
September 2018: Journal of Orthopaedics
Jinxia Peng, Rui Liu, Ling Peng, Hongtao Jia
The present study was performed to determine the molecular mechanism of calcium gluconate (CG) in alleviating the toxic effect of hydrofluoric (HF) acid on human dermal fibroblasts (HDFs). HDF morphology was observed by optical microscopy and the vimentin immunofluorescence assay. Cell viability and apoptosis were evaluated by the Cell Counting Kit-8 and Annexin V/propidium iodide assays, respectively. The levels of apoptosis-associated factors, as well as Wnt2, Wnt3a and β-catenin were detected by reverse transcription-quantitative polymerase chain reaction and western blotting...
September 2018: Oncology Letters
Wei Wei, Fei Jiang, Xiao-Chang Liu, Qian Su
Novel studies have shown that the Transmembrane protein 9 (TMEM9) gene is localized at 1q41 and encodes a protein consisting of 183 amino acids with an N-terminus containing many important domains. As a novel human transmembrane protein, TMEM9 is highly conserved in species from Caenorhabditis elegans to humans and is widely expressed in many tissues and cells. Moreover, TMEM9 may play an important role in intracellular transport and the growth of hepatoma cells. However, evidence for the function of TMEM9 in inflammation is still limited...
October 2018: International Immunopharmacology
Alex Dobzanski, Syed Muaz Khalil, Andrew P Lane
BACKGROUND: While essential to the normal differentiation of ciliated airway epithelial cells, upregulated Wnt signaling in chronic rhinosinusitis with nasal polyps (CRSwNP) has been proposed to result in abnormal epithelial morphology and dysfunctional mucociliary clearance. The mechanism of epithelial Wnt signaling dysregulation in CRSwNP is unknown, and importantly cellular sources of Wnt ligands in CRSwNP have not yet been investigated. METHODS: Human sinonasal epithelial cells (hSNECs) and human sinonasal fibroblasts (hSNFs) were collected from 34 human subjects (25 control and 9 CRSwNP) and differentiated as primary air-liquid interface (ALI) and organoid co-cultures...
August 17, 2018: International Forum of Allergy & Rhinology
Alexey Koval, Constant A Pieme, Emerson Ferreira Queiroz, Simone Ragusa, Kamal Ahmed, Artem Blagodatski, Jean-Luc Wolfender, Tatiana V Petrova, Vladimir L Katanaev
Triple-negative breast cancer (TNBC) and colon cancer (CC) are two stigmatic examples of poorly treatable tumors, whose progression critically depends upon hyperactivation of the Wnt signaling. Development of specific anti-Wnt inhibitors is required to develop drugs against these and other Wnt-dependent cancers. Natural products, especially plants, have been used for the treatment of various diseases from ancient times. We examined extracts from several indigenous Cameroonian herbs and tested their effects on proliferation and Wnt signaling in TNBC and CC cells...
October 28, 2018: Cancer Letters
Wei Xie, Yan Zhang, Yuan He, Kunchi Zhang, Guoqing Wan, Yanjuan Huang, Zhaoli Zhou, Gang Huang, Jin Wang
Triple negative breast cancer (TNBC) is one of the most difficult malignancy to treat due to a lack of targeted therapy. Studies have demonstrated that the activation of Wnt/β-catenin signaling was preferentially found in TNBC. Frizzled-7 (Fzd7), one of the Wnt receptors, was significantly up-regulated in TNBC and modulated TNBC tumorigenesis through the Wnt signaling pathway, indicating Fzd7 is a biomarker and a potential therapeutic target for TNBC. Here, we designed a recombinant soluble peptide fragment (rhFzd7) to antagonize Fzd7 by competitively binding with Wnt ligands...
August 7, 2018: International Journal of Biochemistry & Cell Biology
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