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aroma profiles transcript

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https://www.readbyqxmd.com/read/30018610/-gpd1-and-adh3-natural-variants-underlie-glycerol-yield-differences-in-wine-fermentation
#1
Sebastián M Tapia, Mara Cuevas, Valentina Abarca, Verónica Delgado, Vicente Rojas, Verónica García, Claire Brice, Claudio Martínez, Francisco Salinas, Luis F Larrondo, Francisco A Cubillos
Glycerol is one of the most important by-products of alcohol fermentation, and depending on its concentration it can contribute to wine flavor intensity and aroma volatility. Here, we evaluated the potential of utilizing the natural genetic variation of non-coding regions in budding yeast to identify allelic variants that could modulate glycerol phenotype during wine fermentation. For this we utilized four Saccharomyces cerevisiae strains (WE - Wine/European, SA - Sake, NA - North American, and WA - West African), which were previously profiled for genome-wide Allele Specific Expression (ASE) levels...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29967718/transcriptome-profiling-by-rna-seq-reveals-differentially-expressed-genes-related-to-fruit-development-and-ripening-characteristics-in-strawberries-fragaria-%C3%A3-ananassa
#2
Panpan Hu, Gang Li, Xia Zhao, Fengli Zhao, Liangjie Li, Houcheng Zhou
Strawberry ( Fragaria × ananassa ) is an ideal plant for fruit development and ripening research due to the rapid substantial changes in fruit color, aroma, taste, and softening. To gain deeper insights into the genes that play a central regulatory role in strawberry fruit development and ripening characteristics, transcriptome profiling was performed for the large green fruit, white fruit, turning fruit, and red fruit stages of strawberry. A total of 6,608 differentially expressed genes (DEGs) with 2,643 up-regulated and 3,965 down-regulated genes were identified in the fruit development and ripening process...
2018: PeerJ
https://www.readbyqxmd.com/read/29706329/study-of-lactococcus-lactis-during-advanced-ripening-stages-of-model-cheeses-characterized-by-gc-ms
#3
Marianna Ruggirello, Manuela Giordano, Marta Bertolino, Ilario Ferrocino, Luca Cocolin, Paola Dolci
Lactococcus lactis, is extensively used as starter culture in dairy products. Nevertheless, it has recently been detected in cheese, as metabolically active cells, in advanced ripening stages. In this study, we assessed the viability of L. lactis subsp. lactis in model cheeses up to 180 days of ripening by both culture-dependent and -independent methods. In addition, we studied the expression of metC and als genes involved in the production of aroma compounds detected by Gas Chromatography-Mass Spectrometry (GC-MS)...
September 2018: Food Microbiology
https://www.readbyqxmd.com/read/29371663/high-resolution-spatiotemporal-transcriptome-mapping-of-tomato-fruit-development-and-ripening
#4
Yoshihito Shinozaki, Philippe Nicolas, Noe Fernandez-Pozo, Qiyue Ma, Daniel J Evanich, Yanna Shi, Yimin Xu, Yi Zheng, Stephen I Snyder, Laetitia B B Martin, Eliel Ruiz-May, Theodore W Thannhauser, Kunsong Chen, David S Domozych, Carmen Catalá, Zhangjun Fei, Lukas A Mueller, James J Giovannoni, Jocelyn K C Rose
Tomato (Solanum lycopersicum) is an established model for studying fruit biology; however, most studies of tomato fruit growth and ripening are based on homogenized pericarp, and do not consider the internal tissues, or the expression signatures of individual cell and tissue types. We present a spatiotemporally resolved transcriptome analysis of tomato fruit ontogeny, using laser microdissection (LM) or hand dissection coupled with RNA-Seq analysis. Regulatory and structural gene networks, including families of transcription factors and hormone synthesis and signaling pathways, are defined across tissue and developmental spectra...
January 25, 2018: Nature Communications
https://www.readbyqxmd.com/read/29338343/molecular-analysis-of-anthocyanin-biosynthesis-pathway-genes-and-their-differential-expression-in-mango-peel
#5
Anju Bajpai, Kasim Khan, M Muthukumar, S Rajan, N K Singh
Mango fruit is cherished by masses for its taste and nutrition, contributed by color, flavor, and aroma. Among these, peel color is an important trait contributing to fruit quality and market value. We attempted to elucidate the role of key genes of the anthocyanin biosynthesis pathway related to fruit peel color from the leaf transcriptome of mango cultivar Amrapali. A total of 108 mined transcript sequences were assigned to the phenylpropanoid-flavonoid pathway from which 15 contigs representing anthocyanin biosynthesis genes were annotated...
March 2018: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28764010/effects-of-sunlight-exclusion-on-the-profiles-of-monoterpene-biosynthesis-and-accumulation-in-grape-exocarp-and-mesocarp
#6
Erpeng Zhang, Fengmei Chai, Haohao Zhang, Shaohua Li, Zhenchang Liang, Peige Fan
Terpenes are important aroma compounds in table Muscat grape and wine, and their content in the berry can be affected by sunlight. The effects of sunlight exclusion on monoterpene profiles and relevant gene expression profiles in the exocarp and mesocarp of table Muscat grape 'Jingxiangyu' at different development stages were thoroughly surveyed by bagging pre-veraison clusters in special opaque boxes. The responses of monoterpenes to sunlight treatments varied in three types, representatively linalool, ocimene and geraniol...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28633312/the-influence-of-dekkera-bruxellensis-on-the-transcriptome-of-saccharomyces-cerevisiae-and-on-the-aromatic-profile-of-synthetic-wine-must
#7
Janez Kosel, Neža Cadež, Dorit Schuller, Laura Carreto, Ricardo Franco-Duarte, Peter Raspor
A double compartment membrane system was constructed in order to systematically study possible microbial interactions between yeasts Saccharomyces cerevisiae and Dekkera bruxellensis and their impact on wine aroma. The presence of D. bruxellensis induced 77 transcripts of S. cerevisiae. These were mostly of unknown function; however, some were involved in thiamine biosynthesis and in amino acid and polyamine transport, suggesting a competitive relationship between the two yeast species. Among the transcripts with no biological function, 14 of them were found to be the members of the PAU gene family that is associated with response to anaerobiosis stress...
June 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28559906/drawing-links-from-transcriptome-to-metabolites-the-evolution-of-aroma-in-the-ripening-berry-of-moscato-bianco-vitis-vinifera-l
#8
Laura Costantini, Christian D Kappel, Massimiliano Trenti, Juri Battilana, Francesco Emanuelli, Maddalena Sordo, Marco Moretto, Céline Camps, Roberto Larcher, Serge Delrot, Maria S Grando
Monoterpenes confer typical floral notes to "Muscat" grapevine varieties and, to a lesser extent, to other aromatic non-Muscat varieties. Previous studies have led to the identification and functional characterization of some enzymes and genes in this pathway. However, the underlying genetic map is still far from being complete. For example, the specific steps of monoterpene metabolism and its regulation are largely unknown. With the aim of identifying new candidates for the missing links, we applied an integrative functional genomics approach based on the targeted metabolic and genome-wide transcript profiling of Moscato Bianco ripening berries...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28338755/red-blotch-disease-alters-grape-berry-development-and-metabolism-by-interfering-with-the-transcriptional-and-hormonal-regulation-of-ripening
#9
Barbara Blanco-Ulate, Helene Hopfer, Rosa Figueroa-Balderas, Zirou Ye, Rosa M Rivero, Alfonso Albacete, Francisco Pérez-Alfocea, Renata Koyama, Michael M Anderson, Rhonda J Smith, Susan E Ebeler, Dario Cantu
Grapevine red blotch-associated virus (GRBaV) is a major threat to the wine industry in the USA. GRBaV infections (aka red blotch disease) compromise crop yield and berry chemical composition, affecting the flavor and aroma properties of must and wine. In this study, we combined genome-wide transcriptional profiling with targeted metabolite analyses and biochemical assays to characterize the impact of the disease on red-skinned berry ripening and metabolism. Using naturally infected berries collected from two vineyards, we were able to identify consistent berry responses to GRBaV across different environmental and cultural conditions...
February 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28329733/comparison-of-transcriptional-expression-patterns-of-carotenoid-metabolism-in-cabernet-sauvignon-grapes-from-two-regions-with-distinct-climate
#10
Wei-Kai Chen, Ke-Ji Yu, Bin Liu, Yi-Bin Lan, Run-Ze Sun, Qiang Li, Fei He, Qiu-Hong Pan, Chang-Qing Duan, Jun Wang
The downstream flux of carotenoid metabolism in grape berries includes the biosynthesis of norisoprenoids, a group of important aroma compounds, and the production of ABA, a well-known plant hormone. This study focused on the transcriptional profiling comparison of genes participating in the biosynthesis of carotenoids, norisoprenoids, and ABA in Vitis vinifera 'Cabernet Sauvignon' grapes at pea size, veraison, and ripening stages. The grapes were obtained from Changli (CL, eastern China) and Gaotai (GT, western China) regions and analyzed using RNA-sequencing technology...
June 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28162767/transcriptome-profiling-of-the-australian-arid-land-plant-eremophila-serrulata-a-dc-druce-scrophulariaceae-for-the-identification-of-monoterpene-synthases
#11
Octavia Natascha Kracht, Ann-Christin Ammann, Julia Stockmann, Daniel Wibberg, Jörn Kalinowski, Markus Piotrowski, Russell Kerr, Thomas Brück, Robert Kourist
Plant terpenoids are a large and highly diverse class of metabolites with an important role in the immune defense. They find wide industrial application as active pharmaceutical ingredients, aroma and fragrance compounds. Several Eremophila sp. derived terpenoids have been documented. To elucidate the terpenoid metabolism, the transcriptome of juvenile and mature Eremophila serrulata (A.DC.) Druce (Scrophulariaceae) leaves was sequenced and a transcript library was generated. We report on the first transcriptomic dataset of an Eremophila plant...
April 2017: Phytochemistry
https://www.readbyqxmd.com/read/28002787/diterpenes-biochemical-profile-and-transcriptional-analysis-of-cytochrome-p450s-genes-in-leaves-roots-flowers-and-during-coffea-arabica-l-fruit-development
#12
Suzana T Ivamoto, Leonardo M Sakuray, Lucia P Ferreira, Cíntia S G Kitzberger, Maria B S Scholz, David Pot, Thierry Leroy, Luiz G E Vieira, Douglas S Domingues, Luiz F P Pereira
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450)...
February 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27744211/functional-genomics-provides-insights-into-the-role-of-propionibacterium-freudenreichii-ssp-shermanii-js-in-cheese-ripening
#13
Teija Ojala, Pia K S Laine, Terhi Ahlroos, Jarna Tanskanen, Saara Pitkänen, Tuomas Salusjärvi, Matti Kankainen, Soile Tynkkynen, Lars Paulin, Petri Auvinen
Propionibacterium freudenreichii is a commercially important bacterium that is essential for the development of the characteristic eyes and flavor of Swiss-type cheeses. These bacteria grow actively and produce large quantities of flavor compounds during cheese ripening at warm temperatures but also appear to contribute to the aroma development during the subsequent cold storage of cheese. Here, we advance our understanding of the role of P. freudenreichii in cheese ripening by presenting the 2.68-Mbp annotated genome sequence of P...
January 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27245662/combined-physiological-transcriptome-and-cis-regulatory-element-analyses-indicate-that-key-aspects-of-ripening-metabolism-and-transcriptional-program-in-grapes-vitis-vinifera-l-are-differentially-modulated-accordingly-to-fruit-size
#14
D C J Wong, R Lopez Gutierrez, N Dimopoulos, G A Gambetta, S D Castellarin
BACKGROUND: In wine grape production, management practices have been adopted to optimize grape and wine quality attributes by producing, or screening for, berries of smaller size. Fruit size and composition are influenced by numerous factors that include both internal (e.g. berry hormone metabolism) and external (e.g. environment and cultural practices) factors. Combined physiological, biochemical, and transcriptome analyses were performed to improve our current understanding of metabolic and transcriptional pathways related to berry ripening and composition in berries of different sizes...
May 31, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27043529/two-%C3%AF-3-fads-are-associated-with-peach-fruit-volatile-formation
#15
Jiao-Jiao Wang, Hong-Ru Liu, Jie Gao, Yu-Ji Huang, Bo Zhang, Kun-Song Chen
Aroma-related volatiles, together with sugars and acids, play an important role in determining fruit flavor quality. Characteristic volatiles of peach fruit are mainly derived from fatty acids such as linoleic acid (18:2) and linolenic acid (18:3). In the present study, six genes encoding fatty acid desaturases (FAD) were cloned, including two ω-6 FAD genes (PpFAD2, PpFAD6) and four ω-3 FAD genes (PpFAD3-1, PpFAD3-2, PpFAD7 and PpFAD8). Heterologous expression of peach FADs in tobacco plants showed that PpFAD3-1, and PpFAD3-2 significantly reduced contents of 18:2, and accumulated significant higher levels of 18:3...
March 29, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26846510/the-peach-hecate3-like-gene-fleshy-plays-a-double-role-during-fruit-development
#16
Alessandro Botton, Angela Rasori, Fiorenza Ziliotto, Annick Moing, Mickaël Maucourt, Stéphane Bernillon, Catherine Deborde, Anna Petterle, Serena Varotto, Claudio Bonghi
Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model able to depict the regulatory networks governing this relevant part of fruit development is still missing. Here we described the peach fruit as a system to link the phenotype of a slow ripening (SR) selection to an altered transcriptional regulation of genes involved in determination of mesocarp cell identity providing insight toward molecular regulation of fruit tissue formation...
May 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/26719904/identification-of-differentially-expressed-genes-associated-with-apple-fruit-ripening-and-softening-by-suppression-subtractive-hybridization
#17
Zongying Zhang, Shenghui Jiang, Nan Wang, Min Li, Xiaohao Ji, Shasha Sun, Jingxuan Liu, Deyun Wang, Haifeng Xu, Sumin Qi, Shujing Wu, Zhangjun Fei, Shouqian Feng, Xuesen Chen
Apple is one of the most economically important horticultural fruit crops worldwide. It is critical to gain insights into fruit ripening and softening to improve apple fruit quality and extend shelf life. In this study, forward and reverse suppression subtractive hybridization libraries were generated from 'Taishanzaoxia' apple fruits sampled around the ethylene climacteric to isolate ripening- and softening-related genes. A set of 648 unigenes were derived from sequence alignment and cluster assembly of 918 expressed sequence tags...
2015: PloS One
https://www.readbyqxmd.com/read/26654856/lilium-floral-fragrance-a-biochemical-and-genetic-resource-for-aroma-and-flavor
#18
Timothy S Johnson, Michael L Schwieterman, Joo Young Kim, Keun H Cho, David G Clark, Thomas A Colquhoun
Hybrid Lilium (common name lily) cultivars are among the top produced domestic fresh cut flowers and potted plants in the US today. Many hybrid Lilium cultivars produce large and showy flowers that emit copious amounts of volatile molecules, which can negatively affect a consumer's appreciation or limit use of the plant product. There are few publications focused on the biochemistry, genetics, and/or molecular regulation of floral volatile biosynthesis for Lilium cultivars. In an initial pursuit to provide breeders with molecular markers for floral volatile biosynthesis, a total of five commercially available oriental and oriental-trumpet hybrid Lilium cultivars were selected for analytical characterization of floral volatile emission...
February 2016: Phytochemistry
https://www.readbyqxmd.com/read/26573226/five-omic-technologies-are-concordant-in-differentiating-the-biochemical-characteristics-of-the-berries-of-five-grapevine-vitis-vinifera-l-cultivars
#19
Ryan Ghan, Steven C Van Sluyter, Uri Hochberg, Asfaw Degu, Daniel W Hopper, Richard L Tillet, Karen A Schlauch, Paul A Haynes, Aaron Fait, Grant R Cramer
BACKGROUND: Grape cultivars and wines are distinguishable by their color, flavor and aroma profiles. Omic analyses (transcripts, proteins and metabolites) are powerful tools for assessing biochemical differences in biological systems. RESULTS: Berry skins of red- (Cabernet Sauvignon, Merlot, Pinot Noir) and white-skinned (Chardonnay, Semillon) wine grapes were harvested near optimum maturity (°Brix-to-titratable acidity ratio) from the same experimental vineyard...
November 16, 2015: BMC Genomics
https://www.readbyqxmd.com/read/26515796/de-novo-assembly-and-characterization-of-the-welsh-onion-allium-fistulosum-l-transcriptome-using-illumina-technology
#20
Xiu-Dong Sun, Xin-Hui Yu, Shu-Mei Zhou, Shi-Qi Liu
Welsh onion (Allium fistulosum L.) has long been cultivated as a vegetable and spice for its flavor and aroma. However, transcriptomic and genomic data for A. fistulosum remain scarce. The goal of this study was to generate transcript sequences for functional genomic analyses, and identify genes potentially involved in sulfur, selenium, and vitamin metabolism. In total, 53,378,674 high-quality reads were generated, and de novo assembly resulted in 103,286 contigs and 53,837 unigenes. The average unigene length was 619 bp with an N50 of 832 bp...
April 2016: Molecular Genetics and Genomics: MGG
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