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grape metabolomic

Claudia Schueuermann, Christopher C Steel, John W Blackman, Andrew C Clark, Lachlan J Schwarz, Javier Moraga, Isidro G Collado, Leigh M Schmidtke
Fungal bunch rot of grapes leads to production of detrimental flavour compounds, some of which are well characterised but others remain unidentified. The current study uses an untargeted metabolomics approach to classify volatile profiles of grape juices based on the presence of different fungal pathogens. Individual grape berries were inoculated with Botrytis cinerea, Penicillium expansum, Aspergillus niger or A. carbonarius. Grape bunches were inoculated and blended with healthy fruit, to provide 10% (w/w) infected juice...
January 1, 2019: Food Chemistry
Yan-Lun Ju, Xiao-Feng Yue, Xian-Fang Zhao, Hui Zhao, Yu-Lin Fang
Grapes are one of the most important fruits because of their economic and nutritional benefits, and grapevines are widely cultivated in arid and semi-arid areas. Therefore, it is critical to study the mechanism by which grapevines respond to water stress. In this research, micro-morphological and metabolomic analyses were conducted to evaluate the effects of water stress on stomatal morphology and volatile compounds extracted from the leaves of grapevine plants. There were two treatments: well-watered plants (watered daily) and drought-stressed plants (no irrigation)...
September 2018: Plant Physiology and Biochemistry: PPB
Baltasar Zepeda, Patricio Olmedo, Troy Ejsmentewicz, Pablo Sepúlveda, Iván Balic, Cristóbal Balladares, Joaquín Delgado-Rioseco, Claudia Fuentealba, Adrián A Moreno, Bruno G Defilippi, Claudio Meneses, Romina Pedreschi, Reinaldo Campos-Vargas
Firm berries are highly appreciated by table grape consumers. Cell wall composition is one of the main factors influencing the firmness of table grape berries. Nevertheless, the biological factors driving changes in berry firmness remain unclear. In the present work, we evaluated the firmness of berries of Vitis vinifera cv. Thompson Seedless. We selected two orchards displaying contrasting berry firmness and evaluated polar metabolites and cell wall composition. Our results suggest that berries from the soft orchard exhibited a higher accumulation of sugars at veraison whereas berries from the hard orchard accumulated the same sugars at harvest plus a higher amount of glucose monosaccharide at the cell wall...
December 1, 2018: Food Chemistry
Julien Bensalem, Stéphanie Dudonné, Nicole Etchamendy, Hermine Pellay, Camille Amadieu, David Gaudout, Séverine Dubreuil, Marie-Eve Paradis, Sonia Pomerleau, Lucile Capuron, Carol Hudon, Sophie Layé, Yves Desjardins, Véronique Pallet
Polyphenols are promising nutritional bioactives exhibiting beneficial effect on age-related cognitive decline. The present study evaluated the effect of a polyphenol-rich extract from grape and blueberry (PEGB) on memory of healthy elderly subjects (60-70 years-old). A bicentric, randomized, double-blind, placebo-controlled trial was conducted with 215 volunteers receiving 600 mg/day of PEGB (containing 258 mg flavonoids) or a placebo for 6 months. The primary outcome was the CANTAB Paired Associate Learning (PAL), a visuospatial learning and episodic memory test...
July 19, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Jiukai Zhang, Qiuhao Yu, Haiyan Cheng, Yiqiang Ge, Han Liu, Xingqian Ye, Ying Chen
Berry fruit juice, which is represented by blueberry and cranberry juice, has become increasingly popular due to its reported nutritional and health benefits. However, in markets, adulteration of berry fruit juice with cheaper substitutes is frequent. In the present study, a metabolomic approach for authentication of berry fruit juices by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) was established. The global characterization of the berry fruit metabolome by information dependent acquisition directed LC-MS/MS coupled to a peak mining workflow by isotope pattern matching was reported...
August 1, 2018: Journal of Agricultural and Food Chemistry
Kévin Billet, Benjamin Houillé, Thomas Dugé de Bernonville, Sébastien Besseau, Audrey Oudin, Vincent Courdavault, Guillaume Delanoue, Laurence Guérin, Marc Clastre, Nathalie Giglioli-Guivarc'h, Arnaud Lanoue
Grape accumulates numerous polyphenols with abundant health benefit and organoleptic properties that in planta act as key components of the plant defense system against diseases. Considerable advances have been made in the chemical characterization of wine metabolites particularly volatile and polyphenolic compounds. However, the metabotyping (metabolite-phenotype characterization) of grape varieties, from polyphenolic-rich vineyard by-product is unprecedented. As this composition might result from the complex interaction between genotype, environment and viticultural practices, a field experiment was setting up with uniform pedo-climatic factors and viticultural practices of growing vines to favor the genetic determinism of polyphenol expression...
2018: Frontiers in Plant Science
Eric Soubeyrand, Sophie Colombié, Bertrand Beauvoit, Zhanwu Dai, Stéphanie Cluzet, Ghislaine Hilbert, Christel Renaud, Lilly Maneta-Peyret, Martine Dieuaide-Noubhani, Jean-Michel Mérillon, Yves Gibon, Serge Delrot, Eric Gomès
Anthocyanin biosynthesis is regulated by environmental factors (such as light, temperature, and water availability) and nutrient status (such as carbon, nitrogen, and phosphate nutrition). Previous reports show that low nitrogen availability strongly enhances anthocyanin accumulation in non carbon-limited plant organs or cell suspensions. It has been hypothesized that high carbon-to-nitrogen ratio would lead to an energy excess in plant cells, and that an increase in flavonoid pathway metabolic fluxes would act as an "energy escape valve," helping plant cells to cope with energy and carbon excess...
2018: Frontiers in Plant Science
Jonathan Parrilla, Cécile Gaillard, Jérémy Verbeke, Mickaël Maucourt, Radoslav A Aleksandrov, Florence Thibault, Pierrette Fleurat-Lessard, Yves Gibon, Dominique Rolin, Rossitza Atanassova
A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media. Cytological characterization, 1 H-NMR analysis of intracellular metabolites, and glycolytic enzyme activities provided evidence for the marked metabolic differences between EC and NEC. The EC were characterized by a moderate and organized cell proliferation, coupled with a low flux through glycolysis, high capacity of phosphoenolpyruvate carboxylase and glucokinase, and high oxygen consumption...
May 2018: FEBS Open Bio
Li-Hua Huang, Ming-Quan Yuan, Xiu-Jin Ao, An-Yun Ren, Han-Bo Zhang, Ming-Zhi Yang
Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work, we used high pressure liquid chromatography (HPLC) to analyze the metabolites of fruit flesh cells of grape treated with dual culture of different endophytic fungal strains (EFS). We observed that the dual-culture with different fungal strains show different metabolites composition in grape cells...
2018: PloS One
Jun Niimi, Oliver Tomic, Tormod Næs, David W Jeffery, Susan E P Bastian, Paul K Boss
The current study determined the applicability of sequential and orthogonalised-partial least squares (SO-PLS) regression to relate Cabernet Sauvignon grape chemical composition to the sensory perception of the corresponding wines. Grape samples (n = 25) were harvested at a similar maturity and vinified identically in 2013. Twelve measures using various (bio)chemical methods were made on grapes. Wines were evaluated using descriptive analysis with a trained panel (n = 10) for sensory profiling. Data was analysed globally using SO-PLS for the entire sensory profiles (SO-PLS2), as well as for single sensory attributes (SO-PLS1)...
August 1, 2018: Food Chemistry
Artur Conde, Andreia Neves, Richard Breia, Diana Pimentel, Lia-Tânia Dinis, Sara Bernardo, Carlos Manuel Correia, Ana Cunha, Hernâni Gerós, José Moutinho-Pereira
Water scarcity is associated with extreme temperatures and high irradiance, and significantly and increasingly affects grapevine yield and quality. In this context, the foliar application of kaolin, a chemically inert mineral that greatly reflects ultraviolet and infrared radiations, as well as, in part, photosynthetically active radiation, has recently been shown to decrease photoinhibition in mature leaves. Here, the influence of this particle film on grapevine leaf metabolome and carbohydrate metabolism was evaluated...
April 2018: Journal of Plant Physiology
Violetta Aru, Klavs Martin Sørensen, Bekzod Khakimov, Torben Bo Toldam-Andersen, Søren Balling Engelsen
This study investigates the metabolome of 26 experimental cool-climate wines made from 22 grape varieties using two different protocols for wine analysis by proton nuclear magnetic resonance (¹H-NMR) spectroscopy. The wine samples were analyzed as-is (wet) and as dried samples. The NMR datasets were preprocessed by alignment and mean centering. No normalization or scaling was performed. The "wet" method preserved the inherent properties of the samples and provided a fast and effective overview of the molecular composition of the wines...
January 13, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
N Reshef, N Agam, A Fait
Warm viticulture regions are associated with inferior wines, resulting from the interaction between microclimate and fruit biochemistry. Solar irradiance triggers biosynthetic processes in the fruit and dominates its thermal balance. Therefore, deciphering its impact on fruit metabolism is pivotal to develop strategies for fruit protection and ameliorate its quality traits. Here, we modified light quality and intensity in the fruit-zone and integrated micrometeorology with grape and wine metabolomics, allowing a complete assessment, from field to bottle...
April 11, 2018: Journal of Agricultural and Food Chemistry
Lucie Pinasseau, Anna Vallverdú-Queralt, Arnaud Verbaere, Maryline Roques, Emmanuelle Meudec, Loïc Le Cunff, Jean-Pierre Péros, Agnès Ageorges, Nicolas Sommerer, Jean-Claude Boulet, Nancy Terrier, Véronique Cheynier
Phenolic compounds represent a large family of plant secondary metabolites, essential for the quality of grape and wine and playing a major role in plant defense against biotic and abiotic stresses. Phenolic composition is genetically driven and greatly affected by environmental factors, including water stress. A major challenge for breeding of grapevine cultivars adapted to climate change and with high potential for wine-making is to dissect the complex plant metabolic response involved in adaptation mechanisms...
2017: Frontiers in Plant Science
Beatriz Vallejo, Cecilia Picazo, Helena Orozco, Emilia Matallana, Agustín Aranda
Glufosinate ammonium (GA) is a widely used herbicide that inhibits glutamine synthetase. This inhibition leads to internal amino acid starvation which, in turn, causes the activation of different nutrient sensing pathways. GA also inhibits the enzyme of the yeast Saccharomyces cerevisiae in such a way that, although it is not used as a fungicide, it may alter yeast performance in industrial processes like winemaking. We describe herein how GA indeed inhibits the yeast growth of a wine strain during the fermentation of grape juice...
September 29, 2017: Scientific Reports
Alejandra Serrano, Carmen Espinoza, Grace Armijo, Claudio Inostroza-Blancheteau, Evelyn Poblete, Carlos Meyer-Regueiro, Anibal Arce, Francisca Parada, Claudia Santibáñez, Patricio Arce-Johnson
Grapevine fruit development is a dynamic process that can be divided into three stages: formation (I), lag (II), and ripening (III), in which physiological and biochemical changes occur, leading to cell differentiation and accumulation of different solutes. These stages can be positively or negatively affected by multiple environmental factors. During the last decade, efforts have been made to understand berry development from a global perspective. Special attention has been paid to transcriptional and metabolic networks associated with the control of grape berry development, and how external factors affect the ripening process...
2017: Frontiers in Plant Science
Maryline Magnin-Robert, Marielle Adrian, Sophie Trouvelot, Alessandro Spagnolo, Lucile Jacquens, Patricia Letousey, Fanja Rabenoelina, Mourad Harir, Chloé Roullier-Gall, Christophe Clément, Philippe Schmitt-Kopplin, Armelle Vallat, Eliane Abou-Mansour, Florence Fontaine
Esca disease is one of the major grapevine trunk diseases in Europe and the etiology is complex, since several inhabiting fungi are identified to be associated with this disease. Among the foliar symptom expressions, the apoplectic form may be distinguished and characterized by sudden dieback of shoots, leaf drop, and shriveling of grape clusters in a few days that can ultimately induce the plant death. To further understand this drastic event, we conducted transcriptomic and metabolomic analyses to characterize responses of leaves during the period preceding symptom appearance (20 and 7 days before foliar symptom expression) and at the day of apoplexy expression...
December 2017: Molecular Plant-microbe Interactions: MPMI
Lei Wang, Xiaoliang Sun, Jakob Weiszmann, Wolfram Weckwerth
Grapevine is a fruit crop with worldwide economic importance. The grape berry undergoes complex biochemical changes from fruit set until ripening. This ripening process and production processes define the wine quality. Thus, a thorough understanding of berry ripening is crucial for the prediction of wine quality. For a systemic analysis of grape berry development we applied mass spectrometry based platforms to analyse the metabolome and proteome of Early Campbell at 12 stages covering major developmental phases...
2017: Frontiers in Plant Science
Silvia Ruocco, Marco Stefanini, Jan Stanstrup, Daniele Perenzoni, Fulvio Mattivi, Urska Vrhovsek
Wild American genotypes represent an important part of the Vitis germplasm in relation to grape improvement. Today, these genotypes are currently involved in breeding programmes in order to introgress traits resistant to pests and diseases in V. vinifera cultivars. Nevertheless, the metabolic composition of their grapes has not been widely investigated. This study aimed to explore in detail the metabolomic profile in terms of simple phenolic, proanthocyanidin, anthocyanin and lipid compounds in two hybrids and five American genotypes...
August 2017: Food Research International
Alessandra Rinaldi, Clizia Villano, Carmine Lanzillo, Angelo Tamburrino, Michael Jourdes, Pierre-Louis Teissedre, Luigi Moio, Luigi Frusciante, Domenico Carputo, Riccardo Aversano
Aglianico grapes are known for their high content of proanthocyanidins (PAs), which are responsible for the astringency of wines derived from this cultivar. However, the accumulation of PAs and their genetic control during berry development remain largely unexplored. This work aimed to monitor astringency-causing PAs in Aglianico berries and correlate them with the expression of 14 key genes. Berries were collected during ripening and dissected in skins and seeds. PAs were fractionated and the content of total phenolics, flavans, anthocyanins, tannins reactive towards salivary proteins and tannin structural composition were evaluated...
October 15, 2017: Food Chemistry
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