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https://www.readbyqxmd.com/read/30552671/downregulation-of-long-noncoding-rna-dgcr5-contributes-to-the-proliferation-migration-and-invasion-of-cervical-cancer-by-activating-wnt-signaling-pathway
#1
Yun Liu, Yue Chang, Shuxiong Lu, Yuan-Yuan Xiang
Accumulating research works have reported that long noncoding RNAs (lncRNAs) are involved in various cancers, including cervical cancer. LncRNA DGCR5 has been identified in many cancers. However, the biological role of DGCR5 in cervical cancer remains barely known. We aimed to investigate the biological function of DGCR5 in cervical cancer progression. Here, in our current study, we observed that DGCR5 was downregulated in human cervical cancer cell lines (MS751, SiHa, HeLa, and HT-3) compared with the primary normal cervical squamous cells (NCSC1 and NCSC2)...
December 14, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30551667/the-impact-of-coffee-and-its-selected-bioactive-compounds-on-the-development-and-progression-of-colorectal-cancer-in-vivo-and-in-vitro
#2
REVIEW
Rafał J Bułdak, Tomasz Hejmo, Marcin Osowski, Łukasz Bułdak, Michał Kukla, Renata Polaniak, Ewa Birkner
Coffee is one of the most popular beverages worldwide. Coffee contains bioactive compounds that affect the human body such as caffeine, caffeic acid, chlorogenic acids, trigonelline, diterpenes, and melanoidins. Some of them have demonstrated potential anticarcinogenic effects in animal models and in human cell cultures, and may play a protective role against colorectal cancer. Colorectal cancer (CRC) is the third leading cause of cancer-related mortality in the USA and other countries. Dietary patterns, as well as the consumption of beverages, may reduce the risk of CRC incidence...
December 13, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30551427/investigation-of-the-antitumor-activity-and-toxicity-of-long-circulating-and-fusogenic-liposomes-co-encapsulating-paclitaxel-and-doxorubicin-in-a-murine-breast-cancer-animal-model
#3
Marina Santiago Franco, Marjorie Coimbra Roque, André Luís Branco de Barros, Juliana de Oliveira Silva, Geovanni Dantas Cassali, Mônica Cristina Oliveira
To associate paclitaxel (PTX) with doxorubicin (DXR) is one of the main chemotherapy strategies for breast cancer (BC) management. Despite the high response rates for this combination, it presents a cardiotoxic synergism, attributed to pharmacokinetic interactions between PTX and both DXR and its metabolite, doxorubicinol. One of the main strategies to minimize the cardiotoxicity of the combination is to extend the interval of time between DXR and PTX administration. However, it has been previously suggested that their co-administration leads to better efficacy compared to their sequential administration...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30550341/distinction-of-sporadic-and-familial-forms-of-als-based-on-mitochondrial-characteristics
#4
Jarosław Walczak, Grażyna Dębska-Vielhaber, Stefan Vielhaber, Jędrzej Szymański, Agata Charzyńska, Jerzy Duszyński, Joanna Szczepanowska
Bioenergetic failure, oxidative stress, and changes in mitochondrial morphology are common pathologic hallmarks of amyotrophic lateral sclerosis (ALS) in several cellular and animal models. Disturbed mitochondrial physiology has serious consequences for proper functioning of the cell, leading to the chronic mitochondrial stress. Mitochondria, being in the center of cellular metabolism, play a pivotal role in adaptation to stress conditions. We found that mitochondrial dysfunction and adaptation processes differ in primary fibroblasts derived from patients diagnosed with either sporadic or familial forms of ALS...
December 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30549031/artocarpin-induces-cell-apoptosis-in-human-osteosarcoma-cells-through-endoplasmic-reticulum-stress-and-reactive-oxygen-species
#5
Chiang-Wen Lee, Miao-Ching Chi, Tsung-Ming Chang, Ju-Fang Liu
Osteosarcoma is a malignant primary bone tumor that responds poorly to both chemotherapy and radiation therapy. However, because of side effects and drug resistance in chemotherapy and the insufficiency of an effective adjuvant therapy for osteosarcoma, it is necessary to research novel treatments. This study was the first to investigate the anticancer effects of the flavonoid derivative artocarpin in osteosarcoma. Artocarpin induced cell apoptosis in three human osteosarcoma cell lines-U2OS, MG63, and HOS...
December 13, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30548797/toxicological-evaluation-of-metal-oxide-nanoparticles-and-mixed-exposures-at-low-doses-using-zebra-fish-and-thp1-cell-line
#6
Jasreen Kaur, Madhu Khatri, Sanjeev Puri
Metal and metal oxide nanoparticles are being used in different industries now-a-days leading to their unavoidable exposure to humans and animals. In the present study, toxicological testing was done using nanoparticles of copper oxide, cerium oxide and their mixture (1:1 ratio) on zebra fish embryos and THP-1 cell line. Zebrafish embryos were exposed to 0.01 μg/ml to 50 μg/ml concentrations of dispersed nanoparticles using a 96 well plate and their effects were studied at different hours post fertilization (hpf) i...
December 12, 2018: Environmental Toxicology
https://www.readbyqxmd.com/read/30546830/inhibition-of-breast-cancer-metastasis-to-the-lungs-with-ubs109
#7
Mamoru Shoji, Wei Ping Qian, Ganji Purnachandra Nagaraju, Daniel J Brat, Danielle Pessolano, Rick Luzietti, Spandan Chennamadhavuni, Masayoshi Yamaguchi, Lily Yang, Dennis C Liotta
Synthetic monocarbonyl analogs of curcumin (MACs) are cytotoxic against several cancers including head and neck cancer, pancreatic cancer, colon cancer, and breast cancer. Mechanisms of action include depolarization of the mitochondrial membrane potential and inhibition of NF-κB, leading to apoptosis. We previously demonstrated that UBS109 (MAC), has preventive effects on bone loss induced by breast cancer cell lines. We determined whether UBS109 could inhibit and prevent lung metastasis, since lung metastasis of breast cancer is a major problem in addition to bone metastasis...
November 16, 2018: Oncotarget
https://www.readbyqxmd.com/read/30544702/bojungikgi-tang-a-traditional-herbal-formula-exerts-neuroprotective-effects-and-ameliorates-memory-impairments-in-alzheimer-s-disease-like-experimental-models
#8
Hye-Sun Lim, Yu Jin Kim, Eunjin Sohn, Jiyeon Yoon, Bu-Yeo Kim, Soo-Jin Jeong
Bojungikgi-tang (BJIGT; Bu Zhong Yi Qi Tang in China, Hochuekkito in Japan) is a traditional Oriental herbal formula comprised of eight medicinal herbs that has long been used for the treatment of digestive disorders. A recent clinical study from South Korea reported that BJIGT-gamibang administration may be effective in treating dementia. We aimed to establish scientific evidence for the anti-dementia effects of BJIGT using in vitro and in vivo experimental models. We measured amyloid- β (Aβ) aggregation, β-secretase (BACE), and antioxidant activity in a cell free system...
December 10, 2018: Nutrients
https://www.readbyqxmd.com/read/30543842/adaptation-of-a-skin-sensitization-assay-to-a-chemically-defined-culture
#9
Bianca Marigliani, Josemar Vinícius Maiworm Abreu Silva, Luciene Bottentuit López Balottin, Karina Ribeiro da Silva, Leandra Santos Baptista, Claudia Barbosa Ladeira de Campos, Elisabeth de Fátima Pires Augusto
There are currently three in vitro methods adopted by the Organization for the Economic Co-operation and Development for testing chemicals based on the third key event of the skin sensitization adverse outcome pathway, the activation of dendritic cells. All of them use culture medium supplemented with fetal bovine serum (FBS), which brings technical disadvantages and animal welfare concerns. The objective of this study was to analyze the possibility of eliminating the use of FBS in the human Cell Line Activation Test (h-CLAT)...
December 10, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30543053/engineering-chlamydomonas-reinhardtii-for-expression-of-functionally-active-human-interferon-%C3%AE
#10
Yassin El-Ayouty, Islam El-Manawy, Sherif Nasih, Emad Hamdy, Rashad Kebeish
Human interferon (IFN) are secreted cytokines that play a major regulatory role in response to various infections. Commercially, IFN-α has been approved to treat many chronic viral diseases as well as a variety of cancers and different types of leukemia. In this study, a binary vector containing human IFN-α2a gene under the regulation of the cauliflower mosaic virus 35S promoter was constructed. IFN-œ2a expression cassette was transferred to Chlamydomonas reinhardtii cells via Agrobacterium-mediated transformation method...
December 12, 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/30542709/apoptosis-induced-by-ursodeoxycholic-acid-in-human-melanoma-cells-through-the-mitochondrial-pathway
#11
Huan Yu, Qi-Rui Fu, Zhi-Jie Huang, Jia-Yu Lin, Qing-Xi Chen, Qin Wang, Dong-Yan Shen
Ursodeoxycholic acid (UDCA) is a type of hydrophilic bile acid extracted from animal bile with a wide range of biological functions. The present results demonstrated that UDCA could effectively inhibit the proliferation of two human melanoma cell line (M14 and A375) with time‑ and concentration‑dependence. Following exposure to various concentrations of UDCA, M14 cells exhibited typical morphological changes and weaker ability of colony forming. Flow cytometry analysis demonstrated that UDCA could induce a decrease of mitochondrial membrane potential and an increase in reactive oxygen species (ROS) levels in M14 cells...
October 29, 2018: Oncology Reports
https://www.readbyqxmd.com/read/30539003/functional-probiotic-assessment-and-in-vivo-cholesterol-lowering-efficacy-of-weissella-sp-associated-with-arid-lands-living-hosts
#12
Imene Fhoula, Amel Rehaiem, Afef Najjari, Donatella Usai, Abdellatif Boudabous, Leonardo Antonio Sechi, Ouzari Hadda-Imene
The research and the selection of novel probiotic strains from novel niches are receiving increased attention on their proclaimed health benefits to both humans and animals. This study aimed to evaluate the functional properties of Weissella strains from arid land living-hosts and to select strains with cholesterol-lowering property in vitro and in vivo , for use as probiotics. They were assessed for acid and bile tolerance, antibiotic susceptibility, membrane properties, antibacterial activity, antiadhesive effect against pathogens to host cell lines, and cholesterol assimilation in vitro ...
2018: BioMed Research International
https://www.readbyqxmd.com/read/30538805/c-met-signaling-protects-from-nonalcoholic-steatohepatitis-nash-induced-fibrosis-in-different-liver-cell-types
#13
Hannah K Drescher, Fabienne Schumacher, Teresa Schenker, Maike Baues, Twan Lammers, Thomas Hieronymus, Christian Trautwein, Konrad L Streetz, Daniela C Kroy
Nonalcoholic steatohepatitis (NASH) is the most common chronic, progressive liver disease in Western countries. The significance of cellular interactions of the HGF/c-Met axis in different liver cell subtypes and its relation to the oxidative stress response remains unclear so far. Hence, the present study is aimed at investigating the role of c-Met and the interaction with the oxidative stress response during NASH development in mice and humans. Conditional c-Met knockout (KO) lines (LysCre for Kupffer cells/macrophages, GFAPCre for α -SMA+ and CK19+ cells and MxCre for bone marrow-derived immune cells) were fed chow and either methionine-choline-deficient diet (MCD) for 4 weeks or high-fat diet (HFD) for 24 weeks...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/30538492/icotinib-inhibits-the-proliferation-of-hepatocellular-carcinoma-cells-in-vitro-and-in-vivo-dependently-on-egfr-activation-and-pdl1-expression
#14
Jisan Sun, Wentao Jiang, Dazhi Tian, Qingjun Guo, Zhongyang Shen
Purpose: Hepatocellular carcinoma (HCC) accounts for one of the most prevalent tumor types in the world and still lacks an effective treatment regimen. The EGFR tyrosine-kinase inhibitor icotinib is capable of inhibiting proliferation of several kinds of cancer cells, but its anticancer effect in HCC is still not verified. Methods: In the current study, ten HCC cell lines were selected to test their original EGFR-activation status and PDL1 protein level, and in vitro antiproliferation assays were also conducted to analyze the IC50 and further investigate the correlation between IC50 and protein level of phosphorylated EGFR and PDL1...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/30537967/tel-evax-a-genetic-vaccine-targeting-telomerase-for-treatment-of-canine-lymphoma
#15
Joseph A Impellizeri, Alessandra Gavazza, Eliana Greissworth, Anna Crispo, Maurizio Montella, Gennaro Ciliberto, George Lubas, Luigi Aurisicchio
BACKGROUND: we have recently shown that Tel-eVax, a genetic vaccine targeting dog telomerase (dTERT) and based on Adenovirus (Ad)/DNA Electro-Gene-Transfer (DNA-EGT) technology can induce strong immune response and increase overall survival (OS) of dogs affected by multicentric Diffuse Large B cell Lymphoma (DLBCL) when combined to COP therapy in a double-arm study. Here, we have utilized a clinically validated device for veterinary electroporation called Vet-ePorator™, based on Cliniporator™ technology currently utilized and approved in Europe for electrochemotherapy applications and adapted to electrogenetransfer (EGT)...
December 11, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/30535317/kidney-toxicity-and-response-of-selenium-containing-protein-glutathione-peroxidase-gpx3-to-cdte-qds-on-different-levels
#16
Lining Zhao, Wansong Zong, Hao Zhang, Rutao Liu
The toxic mechanism of cadmium-quantum dot (Cd-QDs) to organisms is still debating. In this paper, it was found that Cd-QDs could induce adverse effects to kidney by entering into cells in a time and dose manner and disturbing the redox balance in vivo. As a selenium containing protein, glutathione peroxidase3 (Gpx3) plays a crucial role in maintaining the balance of redox system. The decrease of Gpx3 activity might be related to the imbalance of redox system. Similar to the animal results, it was demonstrated that Gpx3 activity is also inhibited by Cd-QDs in vitro...
December 10, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/30533582/indole-alkaloid-derivative-b-a-novel-bifunctional-agent-that-mitigates-5-fluorouracil-induced-cardiotoxicity
#17
Wei Bi, Yue Bi, Pengfei Li, Shanshan Hou, Xin Yan, Connor Hensley, Catherine E Bammert, Yanrong Zhang, K Michael Gibson, Jingfang Ju, Lanrong Bi
Clinically approved therapeutics that mitigate chemotherapy-induced cardiotoxicity, a serious adverse effect of chemotherapy, are lacking. The aim of this study was to determine the putative protective capacity of a novel indole alkaloid derivative B ( IADB ) against 5-fluorouracil (5-FU)-induced cardiotoxicity. To assess the free-radical scavenging activities of IADB , the acetylcholine-induced relaxation assay in rat thoracic aorta was used. Further, IADB was tested in normal and cancer cell lines with assays gauging autophagy induction...
November 30, 2018: ACS Omega
https://www.readbyqxmd.com/read/30531868/gdnf-mediated-rescue-of-the-nigrostriatal-system-depends-on-the-degree-of-degeneration
#18
Luis Quintino, Martino Avallone, Emil Brännstrom, Patrick Kavanagh, Marcus Lockowandt, Patricia Garcia Jareño, Ludivine S Breger, Cecilia Lundberg
Glial cell-line derived neurotrophic factor (GDNF) is a promising therapeutic molecule to treat Parkinson's disease. Despite an excellent profile in experimental settings, clinical trials testing GDNF have failed. One of the theories to explain these negative outcomes is that the clinical trials were done in late-stage patients that have advanced nigrostriatal degeneration and may therefore not respond to a neurotrophic factor therapy. Based on this idea, we tested if the stage of nigrostriatal degeneration is important for GDNF-based therapies...
December 7, 2018: Gene Therapy
https://www.readbyqxmd.com/read/30531713/chromatin-immunoprecipitation-of-murine-brown-adipose-tissue
#19
Maria Dafne Cardamone, Joseph Orofino, Adam Labadorf, Valentina Perissi
Most cellular processes are regulated by transcriptional modulation of specific gene programs. Such modulation is achieved through the combined actions of a wide range of transcription factors (TFs) and cofactors mediating transcriptional activation or repression via changes in chromatin structure. Chromatin immunoprecipitation (ChIP) is a useful molecular biology approach for mapping histone modifications and profiling transcription factors/cofactors binding to DNA, thus providing a snapshot of the dynamic nuclear changes occurring during different biological processes...
November 21, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/30531185/ferroptosis-contributes-to-neuronal-death-and-functional-outcome-after-traumatic-brain-injury
#20
Elizabeth M Kenny, Emin Fidan, Qin Yang, Tamil S Anthonymuthu, Lee Ann New, Elizabeth A Meyer, Hong Wang, Patrick M Kochanek, C Edward Dixon, Valerian E Kagan, Hülya Bayır
OBJECTIVES: Traumatic brain injury triggers multiple cell death pathways, possibly including ferroptosis-a recently described cell death pathway that results from accumulation of 15-lipoxygenase-mediated lipid oxidation products, specifically oxidized phosphatidylethanolamine containing arachidonic or adrenic acid. This study aimed to investigate whether ferroptosis contributed to the pathogenesis of in vitro and in vivo traumatic brain injury, and whether inhibition of 15-lipoxygenase provided neuroprotection...
December 6, 2018: Critical Care Medicine
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