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https://www.readbyqxmd.com/read/30118903/ala-pdt-promotes-hpv-positive-cervical-cancer-cells-apoptosis-and-dcs-maturation-via-mir-34a-regulated-hmgb1-exosomes-secretion
#1
Yichao Jin, Zhen Guan, Xiaochuan Wang, Zhiqiong Wang, Rong Zeng, Liangheng Xu, Ping Cao
5-Aminolevulinic acid photodynamic therapy(ALA-PDT) has been widely used for cervical cancer treatment, but the mechanisms are still not fully delineated. Here we showed that ALA-PDT significantly upregulated HMGB1 while downregulated miR-34a expression levels in cervical cancer tissues, and the percentages of mature DCs(mDCs) were increased in ALA-PDT treated patients' peripheral blood. After treating HPV-positive Hela, SiHa, Caski and HPV-negative C33 A cervical cancer cell lines with ALA-PDT, HPV-positive cells' proliferative ability was significantly inhibited and apoptosis rates were elevated, while no significant changes were found in HPV-negative C33 A cell line...
August 14, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/30118482/a-novel-recombinant-human-plasminogen-activator-efficient-expression-and-hereditary-stability-in-transgenic-goats-and-in-vitro-thrombolytic-bioactivity-in-the-milk-of-transgenic-goats
#2
Zhengyi He, Rui Lu, Ting Zhang, Lei Jiang, Minya Zhou, Daijin Wu, Yong Cheng
BACKGROUND: Thromboses is a rapidly growing medical problem worldwide. Low-cost, high-scale production of thrombotic drugs is needed to meet the demand. The production of biomolecules in transgenic animals might help address this issue. To our knowledge, the expression of recombinant human plasminogen activator (rhPA) in goat mammary glands has never been reported before. METHODS: We constructed a mammary gland-specific expression vector, BLC14/rhPA, which encodes only the essential K2 fibrin-binding and P domains of wild-type tPA (deletion mutant of tPA lacking the F, E, and K1 domains), along with the goat β-lactoglobulin gene signal peptide-coding sequence...
2018: PloS One
https://www.readbyqxmd.com/read/30117420/-exploration-and-characterization-of-a-universal-piggybac-transposon-vector-for-efficient-transgene-studies-in-sheep
#3
Guang Dong Hu, Ke Xing Hao, Tao Huang, Wei Bin Zeng, Xin Li Gu, Jing Wang
piggyBac (PB) is a DNA transposon that mediates transposition in various animal cells. As an efficient tool, PB transposons are applied to various transgenic research and optimized for different species, which is an essential means to enhance its versatility. To construct a universal PB transposase (PBase) vector for ovine transgene manipulation, the gene expression box of PBase with optimized bias for sheep codons was inserted into the pBNW-TP1 vector. The vector pBNW-TP2 with a single plasmid was successfully constructed...
August 16, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/30117040/cervico-vaginal-mucus-cvm-an-accessible-source-of-immunologically-informative-biomolecules
#4
Mounir Adnane, Kieran G Meade, Cliona O'Farrelly
Cervico-vaginal mucus (CVM), the product of epithelial cells lining the uterus, cervix and vagina, is secreted to facilitate uterine lubrication and microbial clearance. Predominantly composed of water and mucins, CVM also contains high levels of immuno-active proteins such as immunoglobulin A (IgA), lactoferrin and lysozyme which protect against infection by blocking adhesion and mediating microbial killing. The repertoire of cytokines, chemokines and antimicrobial peptides is predominantly generated by the secretions of endometrial epithelial cells into the uterine lumen and concentrated in the CVM...
August 16, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/30116759/construction-and-characterization-of-adenovirus-vectors-encoding-aspartate-%C3%AE-hydroxylase-to-preliminary-application-in-immunotherapy-of-hepatocellular-carcinoma
#5
Yujiao Zhou, Feifei Liu, Chengmin Li, Guo Shi, Xiaolei Xu, Xue Luo, Yuanling Zhang, Jingjie Fu, Aizhong Zeng, Limin Chen
Dendritic cells (DCs) harboring tumor-associated antigen are supposed to be a potential immunotherapy for hepatocellular carcinoma (HCC). Aspartate- β -hydroxylase (AAH), an overexpressed tumor-associated cell surface protein, is considered as a promising biomarker and therapeutic target for HCC. In this study, we constructed adenovirus vector encoding AAH gene by gateway recombinant cloning technology and preliminarily explored the antitumor effects of DC vaccines harboring AAH. Firstly, the total AAH mRNA was extracted from human HCC tissues; the cDNA was amplified by RT-PCR, verified, and sequenced after TA cloning...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/30116365/effect-of-lanthanum-chloride-on-tumor-growth-and-apoptosis-in-human-ovarian-cancer-cells-and-xenograft-animal-models
#6
Fen Wang, Yuanfang Zhu, Shanyu Fang, Shuya Li, Sisun Liu
Ovarian cancer is the leading cause of mortality resulting from gynecologic cancer. A common anti-ovarian tumor drug is cisplatin; however, repeated use of cisplatin causes severe resistance and leads to poor long-term survival rate in ovarian cancer patients. Recently, it was reported that lanthanum chloride (LaCl3 ) may inhibit tumor growth and induce apoptosis in certain cancer cells. In the present study, the effect of LaCl3 on ovarian cancer was determined in vivo and in vitro . A cisplatin-sensitive human ovarian cancer cell line, COC1, was used in the current study...
August 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30116204/magnitude-assessment-of-adult-neurogenesis-in-the-octopus-vulgaris-brain-using-a-flow-cytometry-based-technique
#7
Anna Di Cosmo, Carla Bertapelle, Antonio Porcellini, Gianluca Polese
Adult neurogenesis is widespread among metazoans, it occurs in animals with a network nervous system, as cnidarians, and in animals with a complex and centralized brain, such as mammals, non-mammalian vertebrates, ecdysozoans, and a lophotrochozoan, Octopus vulgaris . Nevertheless, there are important differences among taxa, especially in the number of the regions involved and in cell proliferation rate during the life-cycle. The comparative evaluation of adult neurogenesis among different brain regions is an arduous task to achieve with only stereological techniques...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/30115995/desmoglein-2-but-not-desmocollin-2-protects-intestinal-epithelia-from-injury
#8
Annika Gross, Lotta A P Pack, Gabriel M Schacht, Sebastian Kant, Hanna Ungewiss, Michael Meir, Nicolas Schlegel, Christian Preisinger, Peter Boor, Nurdan Guldiken, Claudia A Krusche, Gernot Sellge, Christian Trautwein, Jens Waschke, Arnd Heuser, Rudolf E Leube, Pavel Strnad
Desmosomes are the least understood intercellular junctions in the intestinal epithelia and provide cell-cell adhesion via the cadherins desmoglein (Dsg)2 and desmocollin (Dsc)2. We studied these cadherins in Crohn's disease (CD) patients and in newly generated conditional villin-Cre DSG2 and DSC2 knockout mice (DSG2ΔIEC ; DSC2ΔIEC ). CD patients exhibited altered desmosomes and reduced Dsg2/Dsc2 levels. The intestines of both transgenic animal lines were histopathologically inconspicuous. However, DSG2ΔIEC , but not DSC2ΔIEC mice displayed an increased intestinal permeability, a wider desmosomal space as well as alterations in desmosomal and tight junction components...
August 16, 2018: Mucosal Immunology
https://www.readbyqxmd.com/read/30114639/honokiol-an-anticancer-lignan
#9
REVIEW
Abdur Rauf, Seema Patel, Muhammad Imran, AneelaMaalik, Muhammad Umair Arshad, Farhan Saeed, Yahia N Mabkhot, Salim S Al-Showiman, Nazir Ahmad, Eman Elsharkawy
BACKGROUND: Honokiol ((3',5-di-(2-propenyl)-1,1'-biphenyl-2,2'-diol), a lignan, is a promising antitumor compound, having exerted activity against a number of human cancer cell lines. Honokiol has inhibitory role on the proliferation, invasion and survival of cancer cells in in vitro as well as in vivo studies. It interferes with signaling pathways components in order to elicit the anticancer effect. SCOPE AND APPROACH: In present review, the published data on the efficacy of honokiol against various cancer cell lines and tumor-bearing animal models has been presented and discussed...
August 13, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30113770/gmbtb-poz-a-novel-btb-poz-domain-containing-nuclear-protein-positively-regulates-the-response-of-soybean-to-phytophthora-sojae-infection
#10
Chuanzhong Zhang, Hong Gao, Rongpeng Li, Dan Han, Le Wang, Junjiang Wu, Pengfei Xu, Shuzhen Zhang
Phytophthora sojae is a destructive pathogen of soybean [Glycine max (L.) Merr.] causing stem and root rot to soybean plants worldwide. However, the pathogenesis and molecular mechanism of plant defense responses against P. sojae are largely unclear. Herein, we document the underlying mechanisms and function of a novel BTB/POZ protein in soybean, GmBTB/POZ, which contains a BTB/POZ domain found in certain animal transcriptional regulators, in host soybean plants in response to P. sojae. It is located in the cell nucleus and transcriptionally up-regulated by P...
August 16, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/30111625/models-and-approaches-describing-metabolism-transport-and-toxicity-of-drugs-administered-by-the-ocular-route
#11
Jennifer L Dumouchel, Nagendra Chemuturi, Mark N Milton, Gian Camenisch, James E Chastain, Markus Walles, Vito Sasseville, Mithat Gunduz, Ganesh Iyer, Upendra Argikar
The eye is a complex organ with a series of anatomical barriers that provide protection from physical and chemical injury while maintaining homeostasis and function. The physiology of the eye is multifaceted with dynamic flows and clearance mechanisms. This review highlights that in vitro ocular transport and metabolism models are confined by the availability of clinically relevant absorption, distribution, metabolism, and excretion (ADME) data. In vitro ocular transport models used for pharmacology and toxicity poorly predict ocular exposure...
August 15, 2018: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/30111512/mir-590-5p-suppresses-hepatocellular-carcinoma-chemoresistance-by-targeting-yap1-expression
#12
Minjiang Chen, Liming Wu, Jianfei Tu, Zhongwei Zhao, Xiaoxi Fan, Jianting Mao, Qiaoyou Weng, Xulu Wu, Li Huang, Min Xu, Jiansong Ji
BACKGROUND: Resistance to chemotherapeutic treatment is a common phenomenon in cancers, especially in hepatocellular carcinoma (HCC). The Hippo signaling pathway has been demonstrated to play a role in tumor initiation, development, and progression. However, little is known about its roles in the HCC chemoresistance. METHODS: In this study, real-time PCR and western blotting were used to identify the expression profile of key components of Hippo signaling pathway between chemoresistant and chemosensitive HCC cell lines...
August 13, 2018: EBioMedicine
https://www.readbyqxmd.com/read/30110089/hypericum-perforatum-chronic-treatment-affects-cognitive-parameters-and-brain-neurotrophic-factor-levels
#13
Samira S Valvassori, Cenita Borges, Daniela V Bavaresco, Roger B Varela, Wilson R Resende, Bruna R Peterle, Camila O Arent, Josiane Budni, João Quevedo
OBJECTIVE: To evaluate the effects of Hypericum perforatum (hypericum) on cognitive behavior and neurotrophic factor levels in the brain of male and female rats. METHODS: Male and female Wistar rats were treated with hypericum or water during 28 days by gavage. The animals were then subjected to the open-field test, novel object recognition and step-down inhibitory avoidance test. Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell-line derived neurotrophic factor (GDNF) levels were evaluated in the hippocampus and frontal cortex...
August 9, 2018: Revista Brasileira de Psiquiatria
https://www.readbyqxmd.com/read/30109780/apatinib-targets-both-tumor-and-endothelial-cells-in-hepatocellular-carcinoma
#14
Chaoxu Yang, Shukui Qin
Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed malignancies worldwide with poor prognosis and tends to be hypervascular. Aberrant expression of the vascular endothelial growth factor 2 (VEGFR-2) has been implicated in the progression of HCC and represents a valid target for anticancer therapy. Apatinib, a small molecule inhibitor of VEGFR-2 tyrosine kinase, shows strong antitumor activity in various tumors. This study is designed to evaluate the activity of apatinib on both human umbilical vein vascular endothelial cells (HUVECs) and HCC cell lines (in vitro and in vivo), and also to investigate the characteristics and possible mechanisms underlying these effects by molecular biology methods...
August 14, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/30108970/development-of-a-peptide-based-bifunctional-chelator-conjugated-to-a-cytotoxic-drug-for-the-treatment-of-melanotic-melanoma
#15
Raghuvir H Gaonkar, Rinku Baishya, Brahamacharry Paul, Saikat Dewanjee, Shantanu Ganguly, Mita C Debnath, Soumya Ganguly
The cytotoxic drug gemcitabine (GEM) has been conjugated to receptor-binding peptides to target melanoma tumors. A hexapeptide having a Lys-Gly-His-Lys sequence (pep-1), an octapeptide with an Arg-Gly-Asp-Lys-Gly-His-Lys sequence (pep-2), a GEM-conjugated Lys-Gly-His-Lys peptide (GEM-pep-3) and a GEM-conjugated Asp-Gly-Arg peptide (GEM-pep-4) were synthesized and characterized. In vitro uptake of fluorescently labeled GEM-pep-3 and GEM-pep-4 on B16F10 cells was investigated. Fluorescence microscopy studies demonstrated significant uptake of GEM-pep-3 in the B16F10 mouse melanoma cell line...
May 1, 2018: MedChemComm
https://www.readbyqxmd.com/read/30108683/viral-tumor-antigen-expression-is-no-longer-required-in-radiation-resistant-subpopulation-of-jcv-induced-mouse-medulloblastoma-cells
#16
Martina Donadoni, Rahsan Sariyer, Hassen Wollebo, Anna Bellizzi, Ilker Kudret Sariyer
The human neurotropic polyomavirus JC, JC virus (JCV), infects the majority of human population during early childhood and establishes a latent/persistent infection for the rest of the life. JCV is the etiologic agent of the fatal demyelinating disease of the central nervous system, progressive multifocal leukoencephalopathy (PML) that is seen primarily in immunocompromised individuals. In addition to the PML, JCV has also been shown to transform cells in culture systems and cause a variety of tumors in experimental animals...
March 2018: Genes & Cancer
https://www.readbyqxmd.com/read/30105196/chemopreventive-and-anti-breast-cancer-activity-of-compounds-isolated-from-leaves-of-abrus-precatorius-l
#17
Mohammed Shafi Sofi, M K Sateesh, Mohsin Bashir, Mohd Ashraf Ganie, Shabnum Nabi
The present study focuses on isolation and evaluation of the anti-cancer activity of compounds from the leaves of Abrus precatorius . The bioassay-directed strategy was adopted using chromatographic, gas chromatographic-mass spectrum analysis, nuclear magnetic resonance and X-ray crystallography techniques for purification and characterization of active cytotoxic compounds. Further, MDA-MB-231 breast cancer cell lines and 7,12-dimethylbenz (a) anthracene (DMBA) induced virgin female Sprague Dawley (SD) rats were used for in vitro and in vivo cytotoxicity evaluation...
August 2018: 3 Biotech
https://www.readbyqxmd.com/read/30104209/isomeric-separation-and-recognition-of-anionic-and-zwitterionic-n-glycans-from-royal-jelly-glycoproteins
#18
Alba Hykollari, Daniel Malzl, Barbara Eckmair, Jorick Vanbeselaere, Patrick Scheidl, Chunsheng Jin, Niclas G Karlsson, Iain B H Wilson, Katharina Paschinger
Royal jelly has received attention due to its necessity for the development of queen honeybees as well as claims of benefits on human health; this product of the hypopharyngeal glands of worker bees contains a large number of proteins, some of which have been claimed to have various biological effects only in their glycosylated state. However, although there have been glycomic and glycoproteomic analyses in the past, none of the glycan structures previously defined would appear to have potential to trigger specific biological functions...
August 13, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/30104076/correlation-between-adenosine-triphosphate-atp-binding-cassette-transporter-g2-abcg2-and-drug-resistance-of-esophageal-cancer-and-reversal-of-drug-resistance-by-artesunate
#19
Lei Wang, Liang Liu, Yuetong Chen, Yu Du, Jing Wang, Jianghui Liu
The present study investigated the correlation between the abnormal expression of adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2) in esophageal cancer and the drug resistance of esophageal cancer, the reversal effect in drug resistance of artesunate (Art), and the mechanism underlying esophageal cancer using nude mice with subcutaneous xenograft as an animal model. The gene and protein expression of ABCG2 was detected in 80 cases of esophageal cancer, atypical dysplasia, and normal mucosa...
August 7, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/30103946/upregulated-circular-rna-circ_0016760-indicates-unfavorable-prognosis-in-nsclc-and-promotes-cell-progression-through-mir-1287-gage1-axis
#20
Yongsheng Li, Jiahang Hu, Lijuan Li, Shengyan Cai, Hengchun Zhang, Xianfeng Zhu, Guijie Guan, Xiangmei Dong
Non-small cell lung cancer (NSCLC) is an aggressive malignancy with increasing worldwide incidence and is characterized by dismal prognosis due to its early recurrence and metastasis. Accumulating evidence documented that aberrantly expressed circular RNAs (circRNAs) are critically involved in tumorigenesis. In the current study, we focused on a novel circRNA, circ_0016760 in NSCLC. qRT-PCR was carried out to determine the expression level of circ_0016760 in tissue samples and cell lines. The clinical value of circ_0016760 was also investigated...
August 6, 2018: Biochemical and Biophysical Research Communications
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