Adam Eckhardt, Tomas Novotny, Martina Doubkova, Lucia Hronkova, Ludek Vajner, Statis Pataridis, Daniel Hadraba, Lucie Kulhava, Martin Plencner, Jarmila Knitlova, Jana Liskova, Jiri Uhlik, Marie Zaloudikova, David Vondrasek, Ivan Miksik, Martin Ostadal
Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro-proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side; n = 16), with non-contracted tissue (lateral side; n = 13). We used label-free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin-C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot...
March 2019: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society