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https://www.readbyqxmd.com/read/30121040/ballast-water-treatment-and-bacteria-analysis-of-bacterial-activity-and-diversity-after-treatment-of-simulated-ballast-water-by-electrochlorination-and-uv-exposure
#1
Nanna B Petersen, Torben Madsen, Mikkel A Glaring, Fred C Dobbs, Niels O G Jørgensen
Effects of ballast water (BW) treatment by ultra-violet (UV) light and electrochlorination (EC) on survival, activity and diversity of marine bacterioplankton and release of organic matter from cell damage were examined at discharge in a large-scale BW test facility (250 m3 tanks) at Hundested harbour, Denmark. The tests were performed in accordance with the requirements for type approval testing by International Maritime Organization (IMO) and US Coast Guard. After treatment, the water was held in the tanks for one day (EC) before discharge, or 6 days (UV, including also a final UV re-treatment) before discharge...
August 6, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30121024/rapid-and-non-destructive-discrimination-of-special-grade-flat-green-tea-using-near-infrared-spectroscopy
#2
Chunlin Li, Haowei Guo, Bangzheng Zong, Puming He, Fangyuan Fan, Shuying Gong
Special-grade green tea is a premium tea product with the best rank and high value. Special-grade green tea is normally classified by panel sensory evaluation which is time and sample costly. Near-infrared spectroscopy is considered as a promising rapid and non-destructive analytical technique for food quality evaluation and grading. This study established a discrimination method of special-grade flat green tea using Near-infrared spectroscopy. Full spectrum was used for partial least squares (PLS) modelling to predict the sensory scores of green tea, while specific spectral regions were used for synergy interval-partial least squares (siPLS) modelling...
August 1, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/30121020/cloning-expression-analysis-and-functional-characterization-of-an-interleukin-1-receptor-associated-kinase-4-from-apostichopus-japonicus
#3
Yi Cui, Liting Jiang, Ronglian Xing, Zhengdong Wang, Zhenhui Wang, Yina Shao, Weiwei Zhang, Xuelin Zhao, Chenghua Li
Interleukin 1 receptor-associated kinase 4 (IRAK4) is a key factor in TLR-mediated host immune function. In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 1311 bp encoding a 436-amino-acid (aa) residue polyprotein with the typical death domain (10-113aa) and the kinase domain (160-426aa). The mRNA transcripts of AjIRAK4 displayed constitutively expressed in all examined tissues with highest expression in the muscles (7...
August 15, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/30120804/superoxide-dismutase-from-venom-of-the-ectoparasitoid-scleroderma-guani-inhibits-melanization-of-hemolymph
#4
Nai-Yong Liu, Jing-Mei Huang, Xue-Min Ren, Zhi-Wen Xu, Nai-Sheng Yan, Jia-Ying Zhu
Superoxide dismutase (SOD) known as an important antioxidative stress protein has been recently found in venoms of several parasitoid wasps. However, its functions and characteristics as a virulent factor remain scarcely described. Here, we report the characterization of two venomous SOD genes (SguaSOD1 and SguaSOD3) from the ectoparasitoid, Scleroderma guani. The metal binding sites, cysteine amino acid positions and signature sequences of the SOD family were conserved within SguaSOD1 and SguaSOD3. Relatively high levels of their transcripts were observed in pupae followed a decrease in early adults, after which they had the highest transcriptions, indicating that their productions would be regulated in venom apparatus...
August 17, 2018: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/30120602/genome-wide-analysis-of-oligopeptide-transporters-and-detailed-characterization-of-yellow-stripe-transporter-genes-in-hexaploid-wheat
#5
Anil Kumar, Gazaldeep Kaur, Parul Goel, Kaushal Kumar Bhati, Mandeep Kaur, Vishnu Shukla, Ajay Kumar Pandey
Oligopeptide transporters (OPT) are integral cell membrane proteins that play a critical role in the transport of small peptides, secondary amino acids, glutathione conjugates, and mineral uptake. In the present study, 67 putative wheat yellow stripe-like transporter (YSL) proteins belonging to the subfamily of OPT transporters were identified. Phylogeny analysis resulted in the distribution of wheat YSLs into four discrete clades. The highest number of YSLs was present on the A genome and the chromosome 2 of hexaploid wheat...
August 17, 2018: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/30120365/post-operative-aics-status-in-completely-resected-lung-cancer-patients-with-pre-operative-aics-abnormalities-predictive-significance-of-disease-recurrence
#6
Takashi Anayama, Masahiko Higashiyama, Hiroshi Yamamoto, Shinya Kikuchi, Atsuko Ikeda, Jiro Okami, Toshiteru Tokunaga, Kentaro Hirohashi, Ryohei Miyazaki, Kazumasa Orihashi
The AminoIndexTM Cancer Screening (AICS) system, a plasma-free amino acid (PFAA)-based multivariate discrimination index, is a blood screening test for lung cancer based on the comparison of PFAA concentrations between patients with lung cancer and healthy controls. Pre- and post-operative AICS values were compared among 72 patients who underwent curative resection for lung cancer. Post-operative changes in PFAA concentrations were also evaluated. AICS values were classified as rank A (0.0-4.9), B (5.0-7.9), or C (8...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120350/ectopic-expression-of-mutated-type-2c-protein-phosphatase-osabi-like2-decreases-abscisic-acid-sensitivity-in-arabidopsis-and-rice
#7
Akira Endo, Chika Egawa, Mihoko Oohashi, Ayano Meguro-Maoka, Etsuo Shimosaka, Yutaka Sato
Abscisic acid (ABA) is a phytohormone that is necessary for stress adaptation. Recent studies have reported that attenuated levels of ABA improved grain yield and seedling growth under low temperature in cereals. To improve plant growth under low temperature, we attempted to generate ABA-insensitive transgenic rice by expressing a clade A type 2C protein phosphatase (OsPP2C), OsABIL2, with or without the mutation equivalent to the Arabidopsis abi1-1 mutation. A yeast two-hybrid assay revealed that the interaction between OsABIL2 and a putative rice ABA receptor, OsPYL1, was ABA-dependent, and the interaction was lost with amino acid substitution from glycine to aspartic acid at the 183rd amino acid of the OsABIL2 protein, corresponding to abi1-1 mutation...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120231/multiple-domains-of-bacterial-and-human-lon-proteases-define-substrate-selectivity
#8
Lihong He, Dongyang Luo, Fan Yang, Chunhao Li, Xuegong Zhang, Haiteng Deng, Jing-Ren Zhang
The Lon protease selectively degrades abnormal proteins or certain normal proteins in response to environmental and cellular conditions in many prokaryotic and eukaryotic organisms. However, the mechanism(s) behind the substrate selection of normal proteins remains largely unknown. In this study, we identified 10 new substrates of F. tularensis Lon from a total of 21 candidate substrates identified in our previous work, the largest number of novel Lon substrates from a single study. Cross-species degradation of these and other known Lon substrates revealed that human Lon is unable to degrade many bacterial Lon substrates, suggestive of a "organism-adapted" substrate selection mechanism for the natural Lon variants...
August 17, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/30120214/homozygous-variants-in-kiaa1549-encoding-a-ciliary-protein-are-associated-with-autosomal-recessive-retinitis-pigmentosa
#9
Suzanne E de Bruijn, Sanne K Verbakel, Erik de Vrieze, Hannie Kremer, Frans P M Cremers, Carel B Hoyng, L Ingeborgh van den Born, Susanne Roosing
BACKGROUND: Retinitis pigmentosa (RP) shows substantial genetic heterogeneity. It has been estimated that in approximately 60%-80% of RP cases, the genetic diagnosis can be found using whole exome sequencing (WES). In this study, the purpose was to identify causative variants in individuals with genetically unexplained retinal disease, which included one consanguineous family with two affected siblings and one case with RP. METHODS: To identify the genetic defect, WES was performed in both probands, and clinical analysis was performed...
August 17, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/30120199/identification-and-characterization-of-ubiquitinylation-sites-in-tar-dna-binding-protein-of-43kda-tdp-43
#10
Friederike Hans, Marita Eckert, Felix von Zweydorf, Christian Johannes Gloeckner, Philipp J Kahle
TAR DNA binding protein of 43 kDa (TDP-43) forms pathological aggregates in neurodegenerative diseases, particularly in certain forms of frontotemporal dementia and amyotrophic lateral sclerosis. Pathological modifications of TDP-43 include proteolytic fragmentation, phosphorylation, and ubiquitinylation. A pathognomonic TDP-43 C-terminal fragment (CTF) spanning amino acids 193-414 contains only four lysine residues that could be potentially ubiquitinylated. Here, serial mutagenesis of these four lysines to arginine revealed that not a single residue is responsible for the ubiquitinylation of mCherry-tagged CTF...
August 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30120118/ddvk-a-novel-major-facilitator-superfamily-transporter-essential-for-5-5-dehydrodivanillate-uptake-by-sphingobium-sp-strain-syk-6
#11
Kosuke Mori, Koh Niinuma, Masaya Fujita, Naofumi Kamimura, Eiji Masai
Microbial conversion of lignin-derived aromatics is a promising strategy for industrial utilization of this large biomass resource. However, efficient application requires elucidation of relevant transport and catabolic pathways. In Sphingobium sp. strain SYK-6, most enzyme genes involved in 5,5'-dehydrodivanillate (DDVA) catabolism have been characterized but the transporter has not yet been identified. Here we identified SLG_07710 ( ddvK ) and SLG_07780 ( ddvR ), genes encoding a putative major facilitator superfamily (MFS) transporter and MarR-type transcriptional regulator, respectively...
August 17, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/30119885/effects-of-amino-acid-substitutions-on-the-biological-activity-of-anti-cd20-monoclonal-antibody-produced-by-transgenic-silkworms-bombyx-mori
#12
Michihiko Aoyama, Minoru Tada, Ken-Ichiro Tatematsu, Noritaka Hashii, Hideki Sezutsu, Akiko Ishii-Watabe
Recombinant monoclonal antibodies (mAbs) have been used in various therapeutic applications including cancer therapy. Fc-mediated effector functions play a pivotal role in the tumor-killing activities of some tumor-targeting mAbs, and Fc-engineering technologies with glyco-engineering or amino acid substitutions at the antibody Fc region have been used to enhance cytotoxic activities including antibody-dependent cellular cytotoxicity (ADCC). We previously reported that the mAbs produced using transgenic silkworms showed stronger ADCC activity and lower complement-dependent cytotoxicity (CDC) activity than mAbs derived from Chinese hamster ovary (CHO) cells due to their unique N-glycan structure (lack of core-fucose and non-reducing terminal galactose)...
August 14, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30119669/the-molecular-characteristic-analysis-of-prrsv-gsww-2015-strain-and-its-pathogenicity-to-pigs
#13
Weijie Bai, Zhijia Wang, Pu Sun, Jing Zhang, Huifang Bao, Yimei Cao, Yanyan Chang, Zaixin Liu, Dong Li, Zengjun Lu
BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) is a severe disease, causing great economic losses to the pig industry. The causative agent, porcine reproductive and respiratory syndrome virus (PRRSV) is highly variable. Since the emergence of highly pathogenic PRRSV (HP-PRRSV) in China in 2006, this virus strain has undergone extensive variation. To investigate the genetic variation and pathogenicity of currently isolated PRRSV GSWW/2015 strain, its whole genome was sequenced and analyzed for the specific variation in NSP2, GP3 and GP5 regions...
August 17, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30119658/the-nod1-and-nod2-in-mandarinfish-siniperca-chuatsi-molecular-characterization-tissue-distribution-and-expression-analysis
#14
Tiantian Gu, Lu Lu, Jingwen Wang, Lili Tian, Wenzhi Wei, Xinsheng Wu, Qi Xu, Guohong Chen
BACKGROUND: NOD-like receptors (NLRs) are a family of cytoplasmic pattern recognition receptors (PRRs), of which NOD1 and NOD2, are the main representative members. Many investigations have focused on the role of NOD1 and NOD2 in the innate immune response in Cypriniformes and Siluriformes. As an important economic fish in Perciformes, little is known about the function of NOD1 and NOD2 in mandarinfish (Siniperca chuatsi). RESULTS: The full-length NOD1 and NOD2 cDNA sequence was obtained using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE)...
August 17, 2018: BMC Genetics
https://www.readbyqxmd.com/read/30119035/metabolomics-changes-in-patients-with-papp-a2-deficiency-in-response-to-rhigf1-treatment
#15
Annalaura Mastrangelo, Gabriel Á Martos-Moreno, Francisco J Rupérez, Julie A Chowen, Coral Barbas, Jesús Argente
OBJECTIVE: Mutations in the pregnancy-associated plasma protein A2 (PAPP-A2) gene have recently been shown to cause postnatal growth failure in two prepubertal patients from a non-consanguineous Spanish family due to the resulting decrease in IGF1 bioavailability. Although a specific pharmacological treatment of this entity is yet to be established, both children received progressive subcutaneous doses (40 to 120 μg/kg) of rhIGF1 twice daily for 2 years. The improvements in growth, hyperinsulinemia and bone mineral density have been previously reported...
August 12, 2018: Growth Hormone & IGF Research
https://www.readbyqxmd.com/read/30119033/tmem9-mediates-il-6-and-il-1%C3%AE-secretion-and-is-modulated-by-the-wnt-pathway
#16
Wei Wei, Fei Jiang, Xiao-Chang Liu, Qian Su
Novel studies have shown that the Transmembrane protein 9 (TMEM9) gene is localized at 1q41 and encodes a protein consisting of 183 amino acids with an N-terminus containing many important domains. As a novel human transmembrane protein, TMEM9 is highly conserved in species from Caenorhabditis elegans to humans and is widely expressed in many tissues and cells. Moreover, TMEM9 may play an important role in intracellular transport and the growth of hepatoma cells. However, evidence for the function of TMEM9 in inflammation is still limited...
August 14, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/30118988/synthesis-and-biological-evaluation-of-n-aryl-2-phenyl-hydrazinecarbothioamides-experimental-and-theoretical-analysis-on-tyrosinase-inhibition-and-interaction-with-hsa
#17
Danilo Sousa-Pereira, Otávio Augusto Chaves, Camilla Moretto Dos Reis, Márcia C C de Oliveira, Carlos Maurício R Sant'Anna, José Carlos Netto-Ferreira, Aurea Echevarria
A series of N-aryl-2-phenyl-hydrazinecarbothioamides have been investigated as possible inhibitors of tyrosinase, an enzyme involved in the development of melanomas. The hydrazinecarbothioamides 1-6 were synthesized from the reaction between phenylhydrazine and isothiocyanates, for which three different methods have been employed, namely stirring at room temperature, by microwave irradiation or by mechanochemical grinding. Quantitative yields were obtained for the later technique. Compound 4 showed the best value for tyrosinase inhibition (IC50  = 22...
August 6, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/30118952/evaluation-of-daphnia-magna-metabolic-responses-to-organic-contaminant-exposure-with-and-without-dissolved-organic-matter-using-1-h-nuclear-magnetic-resonance-nmr-based-metabolomics
#18
Vera Kovacevic, André J Simpson, Myrna J Simpson
Previous studies have shown that contaminant toxicity to target organisms is altered by the presence of dissolved organic matter (DOM). Contaminants can bind to DOM and this may alter the bioavailability and subsequent toxicity of the contaminants. However, molecular-level techniques are needed to more closely evaluate the impact of DOM on the sub-lethal biochemical responses to emerging contaminants. To investigate how DOM may alter the metabolic response to organic contaminant exposure, 1 H nuclear magnetic resonance (NMR)-based metabolomics was used to investigate how the metabolome of Daphnia magna changes when Suwannee River DOM (5 mg organic carbon/L) is included in the acute exposure of four contaminants with varying hydrophobicity...
August 13, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/30118947/identification-and-expression-of-transcription-factor-sox2-in-large-yellow-croaker-larimichthys-crocea
#19
Yong-Hua Jiang, Kun-Huang Han, Shu-Hong Wang, Yun Chen, Yi-Lei Wang, Zi-Ping Zhang
As an important transcription and pluripotency factor, Sox2 plays its functions essentially in the regulation of self-renewal and pluripotency of embryonic and neural stem cells, as well as embryogenesis, organogenesis, neurogenesis and regeneration. The data is lacking on Sox2 in large yellow croaker (Larimichthys crocea) (Lc-Sox2) which is a limitation on the generation of induced pluripotent stem cells (iPSCs). In this study, Lc-sox2 was cloned by RACE (rapid amplification of cDNA ends) and analyzed by Bioinformatics...
July 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/30118861/expression-purification-and-molecular-characterization-of-a-novel-transcription-factor-kccbf3-from-kandelia-candel
#20
Zhaokui Du, Junmin Li
Kandelia candel, a major species of mangrove in the tropical and subtropical area, is susceptible to low temperature in winter. K. candel was introduced into Zhejiang Province (the northern margin of South China) several decades ago, and suffered from low temperature causing growth retardation, in server cases, even death. To explore the molecular mechanisms of cold acclimation in K. candel, a novel C-repeat binding factor gene KcCBF3 (Genbank accession no. KF111715) of 729 bp open reading frame (ORF) encoding a protein of 242 amino acid residues was isolated, expressed, purified and characterized...
August 14, 2018: Protein Expression and Purification
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