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"Amino acid"

Alessio Lorusso, Valeria Marini, Annapia Di Gennaro, Gaetano Federico Ronchi, Claudia Casaccia, Grazia Carelli, Giuseppe Passantino, Nicola D'Alterio, Vincenzo D'Innocenzo, Giovanni Savini, Federica Monaco, Daniel L Horton
Here we report studies of the antigenic relationships of West Nile virus (WNV) and Usutu virus (USUV), two zoonotic flaviviruses from Italy, together with a Japanese encephalitis virus (JEV) strain and compared them with their genetic relationships using the immunodominant viral E protein. Thirty-nine isolates and reference strains were inactivated and used to immunize rabbits to produce hyper immune sera. Serum samples were tested by neutralization against all isolates and results visualized by generating antigenic map...
December 2, 2018: Infection, Genetics and Evolution
Zhimeng Lv, Ming Guo, Chenghua Li, Yina Shao, Xuelin Zhao, Weiwei Zhang
Vascular endothelial growth factor (VEGF) is a key conservative regulator of inflammation response by promoting cell proliferation, migration, and vascular permeability. It also induces the release of inflammatory factors in vertebrates. We previously characterized NLR family pyrin domain containing 3 and HMGB3 homology in Apostichopus japonicus, providing the occurrence of inflammation in this species. However, to our knowledge, other inflammation-related molecules, such as VEGF, have rarely been investigated...
December 2, 2018: Developmental and Comparative Immunology
Hui-Hua Ge, Yue Qiu, Zhi-Wei Yi, Run-Ying Zeng, Guang-Ya Zhang
Acidic xylanases possess the unique features necessary for the tolerance of acidic environments, which may have great potentials for industrial purposes. However, factors controlling the pH-dependent stability of xylanases are only partially known. Here we proposed a residue interaction networks based method to analyze the differences of residue interactions between 6 pairs of experimentally verified acidic and neutral xylanases. They had very close numbers of aromatic amino acids, however extremely significant more (p < 0...
December 2, 2018: International Journal of Biological Macromolecules
Tsutomu Ishimaru, Sabiha Parween, Yuhi Saito, Takanari Shigemitsu, Hiromoto Yamakawa, Mikio Nakazono, Takehiro Masumura, Naoko K Nishizawa, Motohiko Kondo, Nese Sreenivasulu
Heat stress occurrence during seed filling forms chalky portion in the limited zone of starchy endosperm of rice grains. In this study, isolation of aleurone, dorsal, central and lateral tissues of developing endosperm by laser-microdissection (LM) coupled with gene expression analysis of 44K microarray was performed to identify key regulatory genes involved in the formation of milky-white (MW) and white-back (WB) grains during heat stress. Gene regulatory network analysis classified the genes changed under heat stress into five modules...
December 4, 2018: Plant & Cell Physiology
Robert J DiMario, Asaph B Cousins
Phosphoenolpyruvate (PEP) carboxylase (PEPc) catalyzes the first committed step of C4 photosynthesis generating oxaloacetate from bicarbonate (HCO3-) and PEP. It is hypothesized that PEPc affinity for HCO3- has undergone selective pressure for a lower KHCO3 (Km for HCO3-) to increase the carbon flux entering the C4 cycle, particularly during conditions that limit CO2 availability. However, the decrease in KHCO3 has been hypothesized to cause an unavoidable increase in KPEP (Km for PEP). Therefore, the amino acid residue S774 in the C4 enzyme, which has been shown to increase KPEP, should lead to a decrease in KHCO3...
December 4, 2018: Journal of Experimental Botany
Jiangying Kong, Zhuo Liu, Feng Cai, Xiaocheng Xu, Jun LiuI
OBJECTIVES: To study the relationship between the Asp1104His polymorphism of the nucleotide excision repair gene ERCC5 and treatment sensitivity to oxaliplatin in patients with advanced colorectal cancer (CRC) in China. METHODS: A group of 226 patients in the Department of Gastrointestinal Oncology at Zhejiang Xiaoshan Hospital from July 2011∼December 2016 and a control group of 226 normal healthy individuals were involved in this study. All patients were first diagnosed with advanced CRC and were treated with oxaliplatin-based chemotherapy...
December 3, 2018: Clinics
Naglezi M Lovatto, Bruno B Loureiro, Dirleise Pianesso, Taida J Adorian, Fernanda R Goulart, Caroline S Speroni, Ana Betine B Bender, Jucieli Müller, Leila P DA Silva
The objective of this study was to evaluate growth and metabolic parameters of silver catfish fed with protein concentrates of sunflower meal (SMPC) and crambe meal (CrMPC). The study evaluated two levels of substitution, where 25 or 50% of animal protein was replaced with plant-based protein. A total of 300 silver catfish (14 ± 0.26 g) were used in five treatments and three replications, in fifteen 280-liter experimental units. The results were submitted to analysis of variance and the means of the control diet was compared to the remaining treatments by Dunnett's test at 5% significance level...
October 2018: Anais da Academia Brasileira de Ciências
Yousheng Rao, Zhangfeng Wang, Wen Luo, Wentao Sheng, Rendian Zhang, Xuewen Chai
In the present study, we carried out an examination of the amino acid usage in the zebra finch (Taeniopygia guttata) proteome. We found that tRNA abundance, base composition, hydrophobicity and aromaticity, protein second structure, cysteine residue (Cys) content and protein molecular weight had significant impact on the amino acid usage of the zebra finch. The above factors explained the total variability of 22.85%, 25.37%, 10.91%, 5.06%, 4.21%, and 3.14%, respectively. Altogether, approximately 70% of the total variability in zebra finch could be explained by such factors...
2018: PloS One
Erich Michel, Oliver Zerbe, Andreas Plückthun
Designed armadillo repeat proteins (dArmRPs) are modular peptide binders composed of N- and C-terminal capping repeats Y and A and a variable number of internal modules M that each specifically recognize two amino acids of the target peptide. Complementary fragments of dArmRPs obtained by splitting the protein between helices H1 and H2 of an internal module show conditional and specific assembly only in presence of a target peptide (Michel et al., 2018). Here, we investigate dArmRP fragments that already spontaneously assemble with high affinity, e...
December 1, 2018: Biological Chemistry
Carlos Rp Dechandt, Tatiane M Vicentini, Guilherme Pauperio Lanfredi, Rui M P da Silva, Enilza Maria Espreafico, José A C de Oliveira, Vitor Marcel Faça, Norberto Garcia-Cairasco, Luciane C Alberici
The Wistar Audiogenic Rat (WAR) is an animal model of tonic-clonic epileptic seizures, developed after genetic selection by sister x brother inbreeding of Wistar rats susceptible to sound stimuli. Although metabolic changes have been described in this strain, nothing, however, is known about its mitochondrial metabolism. Here, we addressed mitochondrial aspects of OXPHOS, oxidative stress, biogenesis and dynamics in liver, skeletal muscle and heart of male WAR, correlating then to physiological aspects of body metabolism...
December 5, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Zhou-Li Huang, Jing Dai, Wen-Hua Luo, Xiang-Gui Wang, Jia-Heng Tan, Shuo-Bin Chen, Zhi-Shu Huang
The arginine/glycine-rich region termed the RGG domain is usually found in G-quadruplex (G4)-binding proteins and is im-portant in G4-protein interactions. Studies on the binding mechanism of RGG domains found that small segments (RGG motif) inside the domain contribute greatly to the G4 binding affinity. However, unlike the entire RGG domains that have been broadly explored, the role of the RGG motif remains obscure, with very limited study. Herein, to clarify the role of the RGG motif in G4-protein interactions, we systematically investigated the binding affinity and mode between RGG-motif peptides and G4s...
December 5, 2018: Journal of the American Chemical Society
Jeong-Mo Choi, Rohit V Pappu
Experimentally derived, amino acid specific backbone dihedral angle distributions are invaluable for modeling data-driven conformational equilibria of proteins and for enabling quantitative assessments of the accuracies of molecular mechanics forcefields. The protein coil library that is extracted from analysis of high-resolution structures of proteins, has served as a useful proxy for quantifying intrinsic and context-dependent conformational distributions of amino acids. However, data that go into coil libraries will have hidden biases, and ad hoc procedures must be used to remove these biases...
December 5, 2018: Journal of Chemical Theory and Computation
Linan Zhang, Jinku Li, Hao Wu, Yingxia Li
Saccharina japonica undergoes an alternating life cycle during which the diploid sporophyte generation alternates with the monoploid gametophyte generation. Saccharina japonica uses the UV sex determination system to determine the sex of its haploid gametophytes. However, the sex-determining genes and the sex-determining mechanisms of kelp gametophytes have not been thoroughly elucidated to date. In this study, a kelp HMG-box-containing gene (SjHMG), which is located within the sex determination region (SDR) of S...
December 5, 2018: Journal of Phycology
H Kramar, H Stepaniuk, N Voloshchuk, I Taran, S Kovalenko
An in-depth study of the pharmacological properties of 4-[4-oxo-(4h)-quinazolin-3-yl]-benzoic acid as an analgesic agent established that it had a sufficiently high analgesic effect on models of somatic and neuropathic pain syndromes. Study objective was to study the mechanisms of analgesic action of PK-66 compound in rats using the pharmacological analysis. We evaluated the mechanisms of analgesic effect of PK-66 (1 mg/kg, intraperitoneal) compound on the thermal irritation model on Hours 1, 2, 4 and 6 after administration of study compounds...
October 2018: Georgian Medical News
Takuya Osakada, Takumi Itakura, Ryo Kenmochi, Kazushige Touhara
Exocrine gland-secreting peptide 22 (ESP22) is a 10-kDa protein secreted in tears of juvenile mice. ESP22 inhibits sexual behaviors in adults, leading to a reduction in reproduction rate. We herein identified the 24 amino acid sequence within ESP22 that was essential for exhibiting sexual rejection activity. This synthesizable peptide can be useful for controlling mouse overpopulation.
December 5, 2018: Bioscience, Biotechnology, and Biochemistry
Mostafa M Amer, Ana C Carrasco, Daniel J Leonard, John W Ward, Jonathan Clayden
Imidazolidinone derivatives of a range of aromatic α-amino acids, on treatment with phosgene and potassium iodide, undergo a mild Bischler-Napieralski-style cyclocarbonylation reaction that generates a tricyclic lactam by insertion of a C═O group between amino acid nitrogen and the ortho position of the aryl substituent. Regio- and diastereoselective functionalization of the lactam generates a library of substituted dihydroisoquinolinones and their congeners in enantioenriched form.
December 5, 2018: Organic Letters
Vaughn Mangal, Benjamin Robert Stenzler, Alexandre J Poulain, Celine Gueguen
The biological mobilization of mercury (Hg) into microbes capable of Hg methylation is one of the limiting steps in the formation of the neurotoxin methylmercury (MeHg). Although algal dissolved organic matter (DOM) has been associated with increased MeHg production, the relationship between bacterial Hg uptake and algal DOM remains unexplored. In this study, we aimed to address how the quantity and quality of DOM freshly harvested from several algae affected the bacterial uptake of Hg using a biosensor capable of functioning both aerobically and anaerobically...
December 5, 2018: Environmental Science & Technology
Alexander J Boddy, Dominic P Affron, Christopher J Cordier, Emma Rivers, Alan C Spivey, James Adam Bull
Methods that provide rapid access to new heterocyclic structures in biologically relevant chemical space provide important opportunities in drug discovery. Here, a strategy is described for the preparation of 2,2-disubstituted azetidines, pyrrolidines, piperidines and azepanes bearing ester and diverse aryl substituents. A one-pot rhodium catalyzed N-H insertion and cyclization sequence uses diazo compounds to stitch together linear 1,m-haloamines (m = 2 to 5) to rapidly assemble 4-, 5-, 6- and 7-membered saturated nitrogen heterocycles in excellent yields...
December 5, 2018: Angewandte Chemie
Congshan Liu, Jiaqing Yao, Jianhai Yin, Jian Xue, Haobing Zhang
Echinococcosis, which causes a high disease burden and is of great public health significance, is caused by the larval stage of Echinococcus species. It has been suggested that tubulin is the target of benzimidazoles, the only drugs for the treatment of echinococcosis. This study evaluated the characteristics of tubulins from Echinococcus granulosus. The full-length cDNAs of E. granulosus α- and β-tubulin isoforms were cloned by reverse transcription PCR from protoscolex RNA. Then, these two tubulin isoforms (α9 and β4 ) were recombinantly expressed as insoluble inclusion bodies in Escherichia coli...
2018: Parasite: Journal de la Société Française de Parasitologie
Guadalupe Navarro, Anukriti Sharma, Lara R Dugas, Terrence Forrester, Jack A Gilbert, Brian T Layden
Malnutrition remains a major health problem in low- and middle-income countries. During low protein intake, <0.67 g/kg/day, there is a loss of nitrogen (N2 ) balance, due to the unavailability of amino acid for metabolism and unbalanced protein catabolism results. However, there are individuals, who consume the same low protein intake, and preserve N2 balance for unknown reasons. A novel factor, the gut microbiota, may account for these N2 balance differences. To investigate this, we correlated gut microbial profiles with the growth of four murine strains (C57Bl6/J, CD-1, FVB, and NIH-Swiss) on protein deficient (PD) diet...
December 2018: Physiological Reports
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