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H Esmaeili, M Hamedi, S A Madani
Enzootic ovine abortion is caused by Chlamydia abortus and may result in abortion among small ruminants during the last 2-3 weeks of pregnancy. Enzootic abortion is diagnosed by isolation of the agent or detection of its nucleic acid in the products of abortion or vaginal excretions of freshly aborted females. Isolation of chlamydial agents in cell culture is the gold standard, so in the present study this method was employed. Twenty-eight vaginal and conjunctival swab samples were selected from ewes and does that had recently aborted...
December 2017: Archives of Razi Institute
Kristina Hubbard, Min Wang, David R Smith
Canine brucellosis is an emerging disease and compatible with a One Health management approach. Previous research has found higher prevalence of Brucella canis in stray dog populations than in owned animals, and shelter dogs may represent a zoonotic risk to pet owners. Dogs may also contract other Brucella spp., including Brucella suis, which is carried by some feral swine in the United States and poses a public health risk. Diagnostic tests for Brucella spp. are imperfect. Misclassification of disease status can result in serious repercussions for canine and human health including the unnecessary euthanasia of false positive dogs or failure to identify and remove false negative dogs from susceptible populations...
November 1, 2018: Preventive Veterinary Medicine
David F Steiner, Robert MacDonald, Yun Liu, Peter Truszkowski, Jason D Hipp, Christopher Gammage, Florence Thng, Lily Peng, Martin C Stumpe
Advances in the quality of whole-slide images have set the stage for the clinical use of digital images in anatomic pathology. Along with advances in computer image analysis, this raises the possibility for computer-assisted diagnostics in pathology to improve histopathologic interpretation and clinical care. To evaluate the potential impact of digital assistance on interpretation of digitized slides, we conducted a multireader multicase study utilizing our deep learning algorithm for the detection of breast cancer metastasis in lymph nodes...
October 12, 2018: American Journal of Surgical Pathology
Mitchell S Cappell, Ismail Hader, Mitual Amin
AIM: To systematically review liver disease associated with hemophagocytic lymphohistiocytosis (HLH), propose reasonable contraindications for liver transplantation for liver failure in HLH, and report an illustrative case. METHODS: Systematic review according to PRISMA guidelines of hepatic manifestations of HLH using computerized literature search via PubMed of articles published since 1980 with keywords ("hemophagocytic lymphohistiocytosis" or "HLH") AND ("liver" or "hepatic")...
September 27, 2018: World Journal of Hepatology
Barnaly Rashid, Jiayu Chen, Ishtiaque Rashid, Eswar Damaraju, Jingyu Liu, Robyn Miller, Oktay Agcaoglu, Theo G M van Erp, Kelvin O Lim, Jessica A Turner, Daniel H Mathalon, Judith M Ford, James Voyvodic, Bryon A Mueller, Aysenil Belger, Sarah McEwen, Steven G Potkin, Adrian Preda, Juan R Bustillo, Godfrey D Pearlson, Vince D Calhoun
Multimodal, imaging-genomics techniques offer a platform for understanding genetic influences on brain abnormalities in psychiatric disorders. Such approaches utilize the information available from both imaging and genomics data and identify their association. Particularly for complex disorders such as schizophrenia, the relationship between imaging and genomic features may be better understood by incorporating additional information provided by advanced multimodal modeling. In this study, we propose a novel framework to combine features corresponding to functional magnetic resonance imaging (functional) and single nucleotide polymorphism (SNP) data from 61 schizophrenia (SZ) patients and 87 healthy controls (HC)...
October 6, 2018: NeuroImage
M Repko, M Filipovič, M Prýmek, M Latalski, A Šprláková-Puková, J Hořák
PURPOSE OF THE STUDY The Growth Guidance System (GGS) represent a relatively new alternative to the traditional growing systems used for the treatment of early onset scoliosis. Ranking among the main aims of this surgical treatment is a three-dimensional correction of the deformity, maintenance of spinal growth and postponement of the necessity of final treatment by spondylodesis. MATERIAL AND METHODS Our study retrospectively evaluates the results of surgical correction in a group of 35 patients treated by GGS technique...
2018: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
Yun Liu, Timo Kohlberger, Mohammad Norouzi, George E Dahl, Jenny L Smith, Arash Mohtashamian, Niels Olson, Lily H Peng, Jason D Hipp, Martin C Stumpe
CONTEXT.—: Nodal metastasis of a primary tumor influences therapy decisions for a variety of cancers. Histologic identification of tumor cells in lymph nodes can be laborious and error-prone, especially for small tumor foci. OBJECTIVE.—: To evaluate the application and clinical implementation of a state-of-the-art deep learning-based artificial intelligence algorithm (LYmph Node Assistant or LYNA) for detection of metastatic breast cancer in sentinel lymph node biopsies...
October 8, 2018: Archives of Pathology & Laboratory Medicine
Thomas George Olsen, B Hunter Jackson, Theresa Ann Feeser, Michael N Kent, John C Moad, Smita Krishnamurthy, Denise D Lunsford, Rajath E Soans
Background: Artificial intelligence is advancing at an accelerated pace into clinical applications, providing opportunities for increased efficiency, improved accuracy, and cost savings through computer-aided diagnostics. Dermatopathology, with emphasis on pattern recognition, offers a unique opportunity for testing deep learning algorithms. Aims: This study aims to determine the accuracy of deep learning algorithms to diagnose three common dermatopathology diagnoses...
2018: Journal of Pathology Informatics
M Mlika, O Lamzirbi, M Limam, N Mejri, S Ben Saad, N Chaouch, K Ben Miled, A Marghli, F Mezni
BACKGROUND: The malignant pleural mesothelioma (MPM) is a rare tumour usually associated to asbestos exposure. The delay between the exposure and the occurrence of the cancer can reach 40 years. This caused the pick of incidence described in many countries including Tunisia. The diagnosis is suspected based on clinical features but positive diagnosis is microscopic. Our aim was to describe the clinical and microscopic features of MPM through a single institution experience. PATIENTS AND METHODS: We conducted a retrospective study about 30 MPM diagnosed over a 20-year-period (1995-2015)...
October 4, 2018: Revue de Pneumologie Clinique
Felicidad Espinoza, Lluis Cecchini, Juan Morote, Ricard Marcos, Susana Pastor
It has been suggested that the frequency of micronuclei (MN) in defoliated urothelial cells could be used as a biomarker for both the potential risk of bladder cancer (BC) and its progression. To prove this we have carried out a large study evaluating the MN frequency in a group of 383 hospital patients submitted to cystoscopy. From them, 77 were negative in their first cystoscopy, and were considered as a reference group; 79 were positive and were classified as patients with tumor; and 227 with previous bladder cancer submitted to follow-up monitoring were negative and classified as BC patients without tumor...
October 4, 2018: Environmental and Molecular Mutagenesis
Prem Chand, Anubha Garg, Vandana Singla, Nisha Rani
Introduction: Breast cancer is leading cancer in women, and the incidence of breast cancer in India is on the rise. The most common histologic type of breast cancer is infiltrating ductal carcinoma. Prognostic and predictive factors are used in the management of breast cancer. Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2/neu) are immunohistochemical markers of prognosis as well as predictors of response to therapy. Aims and Objectives: The study was conducted to evaluate ER, PR, and HER2/neu expressions in invasive ductal carcinomas of the breast by immunohistochemistry, to explore the correlation of these markers to each other and to various clinicopathological parameters: age of the patient, histological grade, tumor size, and lymph node metastasis...
July 2018: Nigerian Journal of Surgery: Official Publication of the Nigerian Surgical Research Society
Evangelos Tsiambas, Christos Riziotis, Nicholas S Mastronikolis, Dimitrios Peschos, Alexandros Mortakis, Grigorios Kyroysis, Stylianos N Mastronikolis, Anna Batistatou, Andreas C Lazaris, Efstratios Patsouris, Vasileios Ragos
BACKGROUND/AIM: p16 (gene locus: 9p21) tumor suppressor gene is considered an important biomarker for the progression and prognosis in a variety of malignancies and pre-cancerous lesions, including high-risk (HR-) human papilloma virus (HPV)-mediated squamous intraepithelial lesions (SILs), based on cytological and the corresponding cervical intraepithelial neoplasia (CIN) histopathological categorization. p16 acts as a cyclin-dependent kinase-4 inhibitor negatively regulating the cell cycle...
October 2018: Anticancer Research
Changchun He, Yanchi Chen, Taorong Jian, Heng Chen, Xiaonan Guo, Jia Wang, Lijie Wu, Huafu Chen, Xujun Duan
Accumulating neuroimaging evidence suggests that abnormal functional connectivity of the default mode network (DMN) contributes to the social-cognitive deficits of autism spectrum disorder (ASD). Although most previous studies relied on conventional functional connectivity methods, which assume that connectivity patterns remain constant over time, understanding the temporal dynamics of functional connectivity during rest may provide new insights into the dysfunction of the DMN in ASD. In this work, dynamic functional connectivity analysis based on sliding time window correlation was applied to the resting-state functional magnetic resonance imaging data of 28 young children with ASD (age range: 3-7 years) and 29 matched typically developing controls (TD group)...
October 1, 2018: Autism Research: Official Journal of the International Society for Autism Research
Michele Boracchi, Salvatore Andreola, Federica Collini, Guendalina Gentile, Giorgio Lucchini, Francesca Maciocco, Gian Attilio Sacchi, Riccardo Zoja
INTRODUCTION: In a previous work, we wanted to evaluate if the histochemical determination of lead in Gunshot Residues (GSR) on firearm wounds could be misled due to possible environmental contamination produced by heavy metals and, in particular, by lead. The Sodium Rhodizonate test and its confirmation test with 5% HCl Sodium Rhodizonate resulted to be negative and therefore we wanted to verify if these techniques were sensible enough in order to evaluate this element. We have assessed, on these same samples, a more sensitive technique, as inductive coupled plasma mass spectrometry (ICP-MS) is...
September 13, 2018: Forensic Science International
Kristina Arion, Tuba Aksoy, Catherine Allaire, Heather Noga, Christina Williams, Mohamed A Bedaiwy, Paul J Yong
STUDY OBJECTIVE: To evaluate point-of-care pre-operative transvaginal ultrasound (TVUS) sliding sign, in comparison to palpation of a nodule on digital pelvic examination, for the prediction of pouch of Douglas (POD) obliteration. DESIGN: Analysis of data from a prospective data registry (Canadian Task Force Classification II-2). SETTING: Tertiary referral center. PATIENTS: Women with suspected endometriosis who had pre-operative pelvic examination and point-of-care TVUS, followed by laparoscopic surgery between August 2015 and December 2016...
September 26, 2018: Journal of Minimally Invasive Gynecology
María Cristina Almazán, Carlos Lorenzo Hoyos, Alejandro Javier Krolewiecki, Silvana Pamela Cajal, Griselda Noemí Copa, Pedro Emanuel Fleitas, Paola Andrea Barroso, Jorge Diego Marco, Julio Rubén Nasser, José Fernando Gil
Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. Leishmania spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through PCR-restriction fragment length polymorphism. One hundred smears positive for microscopy, classified in a semiquantitative scale for amastigote density, were analyzed. Also, Leishmanin skin test (LST) results were included. DNA extraction was carried out applying lysis buffer with proteinase K, and then DNA was amplified with ribosomal internal transcribed spacer 1 primers...
September 24, 2018: American Journal of Tropical Medicine and Hygiene
Irit Rosenhek-Goldian, Nir Kampf, Jacob Klein
Friction at hydrophobic surfaces in aqueous media is ubiquitous ( e.g., prosthetic implants, contact lenses, microfluidic devices, biological tissue) but is not well understood. Here, we measure directly, using a surface force balance, both normal stresses and sliding friction in an aqueous environment between a hydrophilic surface (single-crystal mica) and the stable, molecularly smooth, highly hydrophobic surface of a spin-cast fluoropolymer film. Normal force versus surface separation profiles indicate a high negative charge density at the water-immersed fluoropolymer surface, consistent with previous studies...
October 2, 2018: ACS Nano
Mohamed M Amer, Hoda M Mekky, Aziza M Amer, Hanaa S Fedawy
Aim: The aim of this study was to determine the relationship between phenotypic resistance and genotypic resistance of isolated serotyped pathogenic Escherichia coli isolates from the clinically diseased broiler. Materials and Methods: A total of 160 samples (heart, liver, kidney, and lung) were collected from 18 to 34 days old clinically diseased broiler from 40 broiler farms (3-5 birds/farm) reared in Giza and Kaluobaia Governorates for the isolation of pathogenic E...
August 2018: Veterinary World
Salvatore Tolone, Edoardo Savarino, Giovanni Zaninotto, C Prakash Gyawali, Marzio Frazzoni, Nicola de Bortoli, Leonardo Frazzoni, Gianmattia Del Genio, Giorgia Bodini, Manuele Furnari, Vincenzo Savarino, Ludovico Docimo
Background: Hiatal hernia is diagnosed by barium-swallow esophagogram or esophagogastroduodenoscopy, with possible suboptimal results. High-resolution manometry clearly identifies crural diaphragm and lower esophageal sphincter. Objectives: To assess the diagnostic accuracy of high-resolution manometry in detecting hiatal hernia compared to esophagogram and esophagogastroduodenoscopy, using as reference the surgical in vivo measurement. Methods: Patients were studied with esophagogram, esophagogastroduodenoscopy, high-resolution manometry and in vivo evaluation of the esophago-gastric junction...
August 2018: United European Gastroenterology Journal
Akihiko Yoshida, Kaishi Satomi, Makoto Ohno, Yuko Matsushita, Masamichi Takahashi, Yasuji Miyakita, Nobuyoshi Hiraoka, Yoshitaka Narita, Koichi Ichimura
AIMS: Intraoperative frozen section (FS) is an integral component of clinical practice. As a quality control measure, FS diagnosis is subsequently compared with the findings on a FS control slide. These control slides could be used for immunohistochemistry, and the immunohistochemical performance in FS controls is known to be antibody-dependent. Isocitrate dehydrogenase (IDH) is mutated in >80% of lower-grade infiltrating gliomas in adults and ~10% of glioblastomas, with IDH1 (R132H) being the most common...
September 17, 2018: Histopathology
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