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Ying Xu, Yachen Hu, Yaxin Guo, Zihao Zhou, Dan Xiong, Chuang Meng, Qiuchun Li, Shizhong Geng, Zhiming Pan, Xinan Jiao
Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) and biovar Pullorum (S. Pullorum) are gram-negative bacteria, members of the most important infectious pathogens, and have caused common problems in the poultry industry, especially in the developing countries. O- and H-antigen specific anti-sera are commonly for slide and tube agglutination tests to identify Salmonella serovars. However, it is both labor intensive and time consuming, so there is an urgent need for a new technique for the rapid detection of the major Salmonella serovars...
August 7, 2018: Poultry Science
Katharine Spens, Lance Bird, Philip Bright
BACKGROUND: Caesarean section is common in the UK with post-procedural adhesions causing life-long clinical symptoms and impacting future pregnancies. This study's aim was to explore associations between these surgical adhesions, via transabdominal ultrasound findings, and perceived symptoms. METHOD: Females demonstrating 1-3 transverse, lower-segment Caesareans were included. Visceral slide transabdominal ultrasound elicited positive adhesions (<1 cm movement) and negative adhesions (>1 cm movement)...
July 2018: Journal of Bodywork and Movement Therapies
Audree B Tadros, Hannah Y Wen, Monica Morrow
BACKGROUND/OBJECTIVE: Cancers classified as "special histologic subtypes" are felt to have a good prognosis. We used the 21-gene Oncotype DX Breast Recurrence Score® multigene assay to examine prognostic variation within special histologic subtypes. We also examined the Recurrence Score® (RS) distribution among the more common ductal (IDC) and lobular (ILC) cancers. METHODS: 610,350 tumor specimens examined in the Genomic Health clinical laboratory from 2/2004 to 8/2017 were included...
August 9, 2018: Annals of Surgical Oncology
Juan Carlos Quiroz, Elena Geangu, Min Hooi Yong
BACKGROUND: Research in psychology has shown that the way a person walks reflects that person's current mood (or emotional state). Recent studies have used mobile phones to detect emotional states from movement data. OBJECTIVE: The objective of our study was to investigate the use of movement sensor data from a smart watch to infer an individual's emotional state. We present our findings of a user study with 50 participants. METHODS: The experimental design is a mixed-design study: within-subjects (emotions: happy, sad, and neutral) and between-subjects (stimulus type: audiovisual "movie clips" and audio "music clips")...
August 8, 2018: JMIR Mental Health
D Zingue, P Weber, F Soltani, D Raoult, M Drancourt
The laboratory diagnosis of lung mycobacterioses including tuberculosis comprises the microscopic examination of sputum smear after appropriate staining such as Ziehl-Neelsen staining to observe acid-fast bacilli. This standard procedure is operator-dependant and its sensitivity depends on the duration of observation. We developed and evaluated an operator-independent microscopic examination of sputum smears for the automated detection and enumeration of acid-fast bacilli using a ZEISS Axio Scan.Z1 microscope...
July 27, 2018: Scientific Reports
Tonya M Santaus, Shan Li, Paula Ladd, Amanda Harvey, Shannon Cole, O Colin Stine, Chris D Geddes
Sample preparation is a leading bottleneck in rapid detection of pathogenic bacteria. Here, we use Lyse-It® for bacterial cellular lysis, genomic DNA fragmentation, and protein release and degradation for both Listeria monocytogenes and Vibrio cholerae. The concept of Lyse-It® employs a conventional microwave and Lyse-It® slides for intensely focused microwave irradiation onto the sample. High microwave power and a <60 second irradiation time allow for rapid cellular lysis and subsequent intracellular component release...
2018: PloS One
Xunda Luo, Nirag Jhala, Jasvir S Khurana, Christopher Fundakowski, Darshana N Jhala, He Wang
CONTEXT: - Despite the clinical utility of fine-needle aspiration for the diagnosis of salivary pathologies, salivary lesions remain one of the most challenging areas in cytopathology. This is partially because there is no consensus on how to report salivary gland cytopathology, which has resulted in inconsistent terminology among institutions and individual cytopathologists and in confusion in communication among cytopathologists and ordering providers. OBJECTIVE: - To summarize our experience with an institutional salivary gland cytopathology reporting system, as an initiative to promote collaborative work towards a consensus on a reporting system...
July 25, 2018: Archives of Pathology & Laboratory Medicine
Masumi Osugi, Kosei Kinoshita, Atsuro Sugita, Katsumi Kito, Toshiharu Maeda
BACKGROUND: We performed p63 immunostaining to detect myoepithelial cells on BD SurePath liquid-based cytology (LBC) slides and examined whether this improved the diagnostic accuracy in breast fine-needle aspiration cytology (FNAC). METHODS: We examined the diagnostic accuracy using the LBC-p63 immunostaining slides of 298 lesions obtained from July 2010 to August 2016. RESULTS: We defined the cutoff values for malignancy as follows: (1) the percentage of p63+ cluster was <30%, (2) the percentage of p63+ single cells in cell clusters was <3%, (3) the number of p63+ single cells in the background was <20 when the total number of the cell clusters was <100, or the number was <120 when the total number was ≥100...
July 25, 2018: Diagnostic Cytopathology
Tim J van Oostenbrugge, Willemien Runneboom, Elise Bekers, Jan Heidkamp, Johan F Langenhuijsen, Andor Veltien, Arie Maat, Peter F A Mulders, Christina A Hulsbergen-van de Kaa, Jurgen J Fütterer
PURPOSE: To evaluate the feasibility of ex vivo 7T MRI to assess surgical margins (SMs) and pseudocapsule (PC) features after partial nephrectomy (PN). MATERIALS AND METHODS: In this prospective, IRB-approved study, seven patients undergoing a PN for nine tumours between November 2014 and July 2015 were included for analysis after obtaining informed consent. MRI of the specimen was acquired using a 7T small bore scanner. The imaging protocol consisted of anatomical T1-, T2- and diffusion-weighted imaging...
July 24, 2018: European Radiology
Piyumali Sandareka Arachchi, Manjula Manoji Weerasekera, Bimalka Seneviratne, Deepaka Weerasekera, Neluka Fernando, Chinthika Prabhashinie Gunasekara
OBJECTIVE: The study aimed to compare the usefulness of two staining methods for imprint cytology for diagnosis of Helicobacter pylori infection. Gastric biopsy specimens (from dyspeptic patients attending routine upper gastrointestinal endoscopy) were placed on glass slides to obtain imprints. The imprints were stained with Toluidine blue and Giemsa stains separately and observed under ×400 magnification using a light microscope. Imprinted biopsies were sectioned and stained with H & E stain and Giemsa stain for histological analysis...
July 16, 2018: BMC Research Notes
Meru Sheel, Sarah Sheridan, Katherine Gass, Kimberly Won, Saipale Fuimaono, Martyn Kirk, Amor Gonzales, Shannon M Hedtke, Patricia M Graves, Colleen L Lau
INTRODUCTION: Under the Global Programme to Eliminate Lymphatic Filariasis (LF), American Samoa conducted seven rounds of mass drug administration (MDA) from 2000-2006. The World Health Organization recommends systematic post-MDA surveillance using Transmission Assessment Surveys (TAS) for epidemiological assessment of recent LF transmission. We compared the effectiveness of two survey designs for post-MDA surveillance: a school-based survey of children aged 6-7 years, and a community-based survey targeting people aged ≥8 years...
July 2018: PLoS Neglected Tropical Diseases
Shannon Reid, Mercedes Espada, Chuan Lu, George Condous
INTRODUCTION: The study aim was to evaluate the transvaginal sonography (TVS) "sliding sign" alone, direct visualization of the bowel with TVS, and the combination of both methods (i.e. "sliding sign" and direct visualization of the bowel), to determine the optimal TVS method for the prediction of rectal/rectosigmoid deep endometriosis (DE). MATERIAL AND METHODS: Multicentre prospective observational study (January 2009- February 2017). All women underwent TVS to determine whether the "sliding sign" was positive/negative and whether rectal/rectosigmoid DE was present, followed by laparoscopic surgery...
July 14, 2018: Acta Obstetricia et Gynecologica Scandinavica
Ivan Varga, David Kachlík, Marianna Žišková, Michal Miko
The mucosa of uterine tube forms multiple and branched longitudinal mucosal folds and takes part in many reproduction events, such as oocyte pick-up, gamete transport, sperm capacitation, fertilization, and early embryonic development. In the habilitation thesis of German physician Paul Kroemer (1904) was the first to describe the lymphatic lacunae inside the tubal folds (by injection of Indian ink), which the author named the öLymphbahnen" (ölymphatic channels"). Despite the fact that this first description has existed for 110 years, there is no mention of these lacunae in most of the current literature...
July 7, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
Zhengqiang Jiang, Du Q Huynh
We present a multiple pedestrian tracking method for monocular videos captured by a fixed camera in an Interacting Multiple Model (IMM) framework. Our tracking method involves multiple IMM trackers running in parallel which are tied together by a robust data association component. We investigate two data association strategies which take into account both the target appearance and motion errors. We use a 4-dimensional colour histogram as the appearance model for each pedestrian returned by a people detector that is based on the Histogram of Oriented Gradients (HOG) features...
December 4, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
Issam Bennis, Kristien Verdonck, Nora El Khalfaoui, Myriam Riyad, Hajiba Fellah, Jean-Claude Dujardin, Hamid Sahibi, Souad Bouhout, Gert Van der Auwera, Marleen Boelaert
In rural areas in Morocco, diagnosing cutaneous leishmaniasis (CL) can be challenging. We evaluated the accuracy of a rapid diagnostic test (RDT) based on antigen detection, CL Detect Rapid Test™ (Inbios International Inc., Seattle, WA), in this setting. We consecutively recruited patients with new skin ulcers in nine primary health centers. We took a dental broach sample for the RDT and two other tissue samples by scraping the border and center of the lesion with a scalpel and smearing it on a slide. We duplicated each smear by pressing a clean slide against it and processed the slides by using microscopy, polymerase chain reaction (PCR) internal transcribed spacer 1, and kDNA minicircle PCR...
July 9, 2018: American Journal of Tropical Medicine and Hygiene
L Bonetti, N T Haumann, E Brattico, M Kliuchko, P Vuust, T Särkämö, R Näätänen
OBJECTIVE: Memory is the faculty responsible for encoding, storing and retrieving information, comprising several sub-systems such as sensory memory (SM) and working memory (WM). Some previous studies exclusively using clinical population revealed associations between these two memory systems. Here we aimed at investigating the relation between modality-general WM performance and auditory SM formation indexed by magnetic mismatch negativity (MMN) responses in a healthy population of young adults...
July 5, 2018: Brain Research
Maria Beatriz Pena E Silva Leite Nacife, Liliane Maria Vidal Siqueira, Rafael Martins, Valeska Natiely Vianna, Keila Furbino Barbosa, Cássio Zumerle Masioli, Jaime Costa da Silva, George Luiz Lins Machado-Coelho
Intestinal parasitic infections are a common health problem among Amerindian populations and schistosomiasis represents one of the most prevalent diseases in Maxakali people. The Kato-Katz is the diagnostic method recommended by WHO for epidemiological studies; however, one of the technique's limitations is the failure to detect parasites in individuals with low parasite load. The aim of this study was to establish the prevalence of Schistosoma mansoni in indigenous Maxakali villages, evaluating the TF-Test® performance for diagnosis compared to the Kato-Katz technique...
2018: Revista do Instituto de Medicina Tropical de São Paulo
Jonathan J Davick, Henry F Frierson, Mark Smolkin, Alejandro A Gru
Programmed death ligand 1 (PD-L1) is a transmembrane protein that plays a major role in immune suppression. Its interaction with the receptor PD-1 results in downregulation of antitumoral immunity. Humanized monoclonal antibodies that interrupt the PD-L1/PD-1 interaction have shown therapeutic efficacy in patients with advanced urothelial cancer. However, immunohistochemical staining of PD-L1 in bladder tumors and its relationship to tumor histologic type, grade, and overall survival has been incompletely analyzed...
June 30, 2018: Human Pathology
Pushp Lata Sachan, Meenakshi Singh, Munna Lal Patel, Rekha Sachan
Objective: The objective of the study is to evaluate the use of the Pap smear screening method for detection of precancerous lesions. Methods: All women who visited the outpatient gynecology clinic of the Department of Obstetrics and Gynaecology at King Georges Medical University, Lucknow, UP, India, over 1 year for different clinical problems were recruited for the study. A total of 1650 women who were sexually active and over 21 years of age were enrolled in the study...
July 2018: Asia-Pacific Journal of Oncology Nursing
Zhe Zhang, Guangyao Liu, Zhijun Yao, Weihao Zheng, Yuanwei Xie, Tao Hu, Yu Zhao, Yue Yu, Ying Zou, Jie Shi, Jing Yang, Tiancheng Wang, Jing Zhang, Bin Hu
Time-varying connectivity analyses have indicated idiopathic generalized epilepsy (IGE) could cause significant abnormalities in dynamic connective pattern within and between resting-state sub-networks (RSNs). However, previous studies mainly focused on the IGE-induced dynamic changes of functional connectivity (FC) in specific frequency band (0.01-0.08 Hz or 0.01-0.15 Hz), ignoring the changes across different frequency bands. Here, 24 patients with IGE characterized by juvenile myoclonic epilepsy (JME) and 24 matched healthy controls were studied using a data-driven frequency decomposition approach and a sliding window approach...
2018: Frontiers in Neurology
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