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bfgf angiogenesis

Sourik S Ganguly, Paul G Daft, Jingchen Cao, Xiangqi Meng, Zhendong A Zhong, Alexandra Vander Ark, Austin Meadows, Zach Madaj, Bart Williams, Xiaohong Li
The role of myeloid cell-specific TGF-β signaling in non-small-cell lung cancer (NSCLC)-induced osteolytic bone lesion development is unknown. We used a genetically engineered mouse model, Tgfbr2LysMCre knockout (KO), which has a loss of TGF-β signaling specifically in myeloid lineage cells, and we found that the area of H1993 cell-induced osteolytic bone lesions was decreased in Tgfbr2LysMCre KO mice, relative to the area in control littermates. The bone lesion areas were correlated with tumor cell proliferation, angiogenesis, and osteoclastogenesis in the microenvironment...
November 22, 2018: Cancers
Charline Dubois, Natacha Rocks, Silvia Blacher, Irina Primac, Anne Gallez, Melissa García-Caballero, Céline Gérard, Laurent Brouchet, Agnes Noel, Francoise Lenfant, Didier Cataldo, Christel Péqueux
Estrogen signalling pathways are emerging targets for lung cancer therapy. Unravelling the contribution of estrogens in lung cancer development is a pre-requisite to support the development of sex-based treatments and to identify patients who could potentially benefit from anti-estrogen treatments. In this study, we highlight the contribution of lymphatic and blood endothelia in the sex-dependent modulation of lung cancer. The orthotopic graft of syngeneic lung cancer cells into immunocompetent mice showed that lung tumours grew faster in female mice than in males...
November 1, 2018: Endocrine-related Cancer
Kazunori Ono, Kenji Yanishi, Makoto Ariyoshi, Satoshi Kaimoto, Motoki Uchihashi, Keisuke Shoji, Satoaki Matoba
BACKGROUND: Therapeutic angiogenesis with basic fibroblast growth factor (bFGF) with atelocollagen was confirmed in a study using a limb ischemia mouse model. Because the number of elderly patients with critical limb ischemia (CLI) is increasing, particularly that caused by arteriosclerosis obliterans (ASO), the development of less invasive angiogenesis therapies desired. Methods and Results: This first-in-man clinical study was designed to assess the safety and efficacy of i.m. injection of bFGF with atelocollagen...
November 9, 2018: Circulation Journal: Official Journal of the Japanese Circulation Society
Jia Li, Jin Gong, Xiaobing Li, Li Shen, Yewei Xie, Rufang Zhang
OBJECTIVE: Recently, microRNA-34a (miR-34a) has been reported to lead to secretion of proinflammatory cytokine in endothelial cells, whereas whether miR-34a plays a protective role in damaged cardiac microvascular endothelial cells (CMECs) remains to be determined. Herein, the purpose of this study is to explore the effect of miR-34a in mediating Notch signaling pathway in apoptosis and angiogenesis of damaged CMECs. METHODS: The primary mice CMECs were isolated, cultivated, and identified before establishment of damaged CMEC model by incubation with homocysteine (HCY) for 24 hours...
October 18, 2018: Journal of Cellular Biochemistry
Toru Miyanaga, Yoshimichi Ueda, Aiko Miyanaga, Mikio Yagishita, Naoko Hama
To date, studies on mesenchymal tissue stem cells (MSCs) in the perichondrium have focused on in vitro analysis, and the dynamics of cartilage regeneration from the perichondrium in vivo remain largely unknown. We have attempted to apply cell and tissue engineering methodology for ear reconstruction using cultured chondrocytes. We hypothesized that by inducing angiogenesis with basic fibroblast growth factor (bFGF), MSCs or cartilage precursor cells would proliferate and differentiate into cartilage in vivo and that the regenerated cartilage would maintain its morphology over an extended period...
2018: Cellular & Molecular Biology Letters
Yumin Wang, Fan Zhang, Junjun Wang, Lijuan Hu, Feng Jiang, Jian Chen, Jie Chen, Liangxing Wang
Purpose: This study aimed to study the biological function and the molecular mechanisms associated with the promotion of angiogenesis by lncRNA LOC100132354 in lung adenocarcinoma (LAD). Patients and methods: The mRNA expression levels of 100 pairs of LAD and normal tissue samples of LOC100132354, vascular endothelial growth factor A (VEGFA), VEGF receptor-2 (VEGFR2), basic fibroblast growth factor (bFGF), and thrombospondin-1 (TSP-1) were analyzed by qPCR. LOC100132354 was knockdown and overexpressed in SPCA-1 and A549 cell lines to analyze the protein and mRNA expression levels of VEGFA, VEGFR2, bFGF, TSP-1, and changes in protein expression levels of Ras, P-A-Raf, P-B-Raf, P-C-Raf, P-Mekl/2, and P-Erk1/2...
2018: Cancer Management and Research
Denise R Cooper, Chunyan Wang, Rehka Patel, Andrea Trujillo, Niketa A Patel, Jamie Prather, Lisa J Gould, Mack H Wu
Objective: Chronically ill patients heal recalcitrant ulcerative wounds more slowly. Human adipose-derived stem cells (hADSCs) play an important role in tissue regeneration and exosomes secreted by hADSC contribute to their paracrine signaling. In addition to cytokines, lipids and growth factors, hADSC secrete mRNA, miRNA, and long noncoding (lnc) RNA into exosomes. In this study we examined the role of lncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1), an abundant lncRNA in exosomes from conditioned media (CM), on cell migration and ischemic wound healing...
September 1, 2018: Advances in Wound Care
Justyna Niderla-Bielińska, Krzysztof Bartkowiak, Bogdan Ciszek, Ewa Jankowska-Steifer, Alicja Krejner, Anna Ratajska
Sulodexide (SDX) is a mixed drug containing low-molecular-weight heparin sulfate and dermatan sulfate. It exerts mild anticoagulant action but can also affect leukocytes, macrophages, and cell-cell adhesion and may interact with growth factors although its direct influence on endothelial cells is not well described. Clinically, SDX is used for the treatment of cardiovascular diseases, where it exerts anti-inflammatory and endothelial protective effects. The aim of this study was to determine the influence of SDX on tubule formation and angiogenesis-related proteins' mRNA expression in endothelial cell line C166 and mouse proepicardial explants...
September 24, 2018: Fundamental & Clinical Pharmacology
Qinshuo Zhao, Jingqiu Huang, Dong Wang, Liang Chen, Dating Sun, Chunxia Zhao
BACKGROUND: Myocardial infarction (MI) contributes to the development of cardiac remodeling and heart failure. Insufficient post-MI myocardial angiogenesis has been identified as a non-negligible event which precipitates heart failure progression. Previous studies reported that cytochrome P450 epoxygenase and its metabolites exerted beneficial effects on cardiovascular diseases. However, the role of cytochrome P450 2J2 (CYP2J2) in post-MI heart failure is incompletely understood. METHODS AND RESULTS: First, western blot and real-time PCR analyses showed that CYP2J2 expression increased clearly in patients with acute MI and old MI, compared to control...
October 2018: Journal of Molecular and Cellular Cardiology
Qing-Hua Lin, Wei Qu, Jian Xu, Feng Feng, Ming-Fang He
Angiogenesis is a crucial process in the development of inflammatory diseases, including cancer, psoriasis and rheumatoid arthritis. Recently, several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model, and the results indicated that 1-methoxycarbony-β-carboline (MCC) could effectively inhibit blood vessel formation. In this study, we further confirmed that MCC can inhibit, in a concentration-dependent manner, the viability, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro, as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo...
August 2018: Chinese Journal of Natural Medicines
Ying Zhao, Qiang Wang, Yuan Jin, Yadan Li, Changjun Nie, Peipei Huang, Zhixin Li, Bihui Zhang, Zijian Su, An Hong, Xiaojia Chen
BACKGROUND/AIMS: How to aid recovery from severe skin injuries, such as burns, chronic or radiation ulcers, and trauma, is a critical clinical problem. Current treatment methods remain limited, and the discovery of ideal wound-healing therapeutics has been a focus of research. Functional recombinant proteins such as basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) have been developed for skin repair, however, some disadvantages in their use remain. This study reports the discovery of a novel small peptide targeting fibroblast growth factor receptor 2 IIIc (FGFR2IIIc) as a potential candidate for skin wound healing...
2018: Cellular Physiology and Biochemistry
Wangping Hao, Jie Han, Yun Chu, Lei Huang, Yan Zhuang, Jie Sun, Xiaoran Li, Yannan Zhao, Yanyan Chen, Jianwu Dai
Basic fibroblast growth factor (bFGF) is an important protein for wound healing and angiogenesis in tissue engineering, but the lack of a viable delivery system hampers its clinical application. This study aims to maintain the long-term controlled release of bFGF by utilizing a collagen/heparin bi-affinity multilayer delivery system (CHBMDS), which is fabricated by the alternate deposition of negatively charged heparin, positively charged collagen, and CBD-bFGF (a collagen-binding domain [CBD] was fused into the native bFGF) via specific or electrostatic interaction...
August 29, 2018: Macromolecular Bioscience
Fang Shi, Jinglei Li, Ziyang Ye, Liuqing Yang, Tingting Chen, Xue Chen, Ming Ye
In the present study, we investigated the anti-tumor activities of the intracellular homogeneous melanin (LM) of Lachnum YM226 and its derivative (ALM) on liver cancer using murine H22 hepatocarcinoma model. The results showed that LM and ALM (50 and 200 mg kg-1 ) could effectively inhibit tumor growth of H22 tumour-bearing mice. The body weight, liver, spleen and thymus indices also improved in the LM and ALM treated groups. Moreover, the levels of alanine aminotransferase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), creatinine (CRE), blood urea nitrogen (BUN) and uric acid (UA) were lowered...
June 1, 2018: MedChemComm
Jeanne Murphy, Ruth M Pfeiffer, Brittny C Davis Lynn, Ana I Caballero, Eva P Browne, Elizabeth C Punska, Hannah P Yang, Roni T Falk, Douglas L Anderton, Gretchen L Gierach, Kathleen F Arcaro, Mark E Sherman
BACKGROUND: Analysis of cytokines and growth factors in human milk offers a noninvasive approach for studying the microenvironment of the postpartum breast, which may better reflect tissue levels than testing blood samples. Given that Black women have a higher incidence of early-onset breast cancers than White women, we hypothesized that milk of the former contains higher levels of pro-inflammatory cytokines, adipokines, and growth factors. METHODS: Participants included 130 Black and 162 White women without a history of a breast biopsy who completed a health assessment questionnaire and donated milk for research...
November 2018: Breast Cancer Research and Treatment
Xiaojun Fei, Xu Zhang, Qi Wang, Jingbo Li, Hao Shen, Xuanye Wang, Hongquan Liu, Weiwei Tao
Ischemic stroke is an increasingly important public health problem, and no effective treatments are approved. Xijiao Dihuang Decoction (XDD), a famous herbal formula for treating hemorrhagic fever syndromes, has been shown to exert powerful neuroprotective property. The aim of this study was to identify the chemical constituents in XDD, observe the neuroprotective effect of XDD against acute ischemic stroke, and explore the specific mechanisms by which these effects were mediated. With UHPLC-Q/TOF-MS, 47 components in XDD were detected and 25 of them were identified...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Izumi Saito, Takumi Hasegawa, Takeshi Ueha, Daisuke Takeda, Eiji Iwata, Satomi Arimoto, Akiko Sakakibara, Masaya Akashi, Shunsuke Sakakibara, Yoshitada Sakai, Hiroto Terashi, Takahide Komori
INTRODUCTION: Skin flap procedures are widely used to reconstruct skin and soft tissue defects. Skin flap necrosis is a serious postoperative complication. Many researchers have introduced pharmacological agents to improve flap ischemia in experimental studies. However, outcomes of these studies remain controversial. We previously demonstrated that transcutaneous CO2 application improves hypoxia in fracture repair. In this study, we hypothesized that improving hypoxia by transcutaneous CO2 application can improve the blood flow in skin flaps and increase angiogenesis...
November 2018: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Xuefei Shao, Qianxin Hu, Sansong Chen, Qifu Wang, Pengcheng Xu, Xiaochun Jiang
Traumatic brain injury (TBI) is a primary cause of disability and mortality. Ghrelin, a gastrointestinal hormone, has been found to have protective effects for the brain, but the molecular mechanism of these neuroprotective effects of ghrelin remains unclear. In this study, an electronic cortical contusion impactor was used to establish a rat TBI model and we investigated the effect of ghrelin on brain repair by neurological severity score and histological examination. An antibody array was employed to uncover the molecular mechanism of ghrelin's neuroprotective effects by determining the alterations of multiple proteins in the brain cortex...
2018: Frontiers in Neuroscience
Ayaka Tanaka, Hitomi Nakamura, Yasuhiko Tabata, Yuka Fujimori, Keiichi Kumasawa, Tadashi Kimura
AIM: Ovarian tissue cryopreservation before cancer treatment is the only option to preserve fertility under some circumstances. However, tissue ischemia after transplantation while awaiting angiogenesis induces dysfunctional folliculogenesis and reduces ovarian reserve and is one of the disadvantages of frozen-thawed ovarian tissue transplantation. Basic fibroblast growth factor (bFGF) is a major regulator of angiogenesis. However, bFGF rapidly loses biological activity when its free form is injected in vivo...
October 2018: Journal of Obstetrics and Gynaecology Research
Chrissta X Maracle, Kim C M Jeucken, Boy Helder, Thomas M van Gulik, Anne Steins, Hanneke W M van Laarhoven, Sander W Tas
Angiogenesis is essential for colorectal cancer (CRC) progression, as demonstrated by the beneficial clinical effects of therapeutics inhibiting VEGF signaling. However, alternative mechanisms of neovascularization can develop, resulting in treatment failure. Previously we demonstrated NF-κB-inducing kinase (NIK) contributes to pathological angiogenesis. Here, we investigate NIK as a therapeutic target in endothelial cells (EC) in CRC. To determine NIK expression levels in CRC tissues, we immunostained both primary colorectal tumors and tumors metastasized to the liver...
June 19, 2018: Oncotarget
Jing Zhou, Tao Liu, Hao Guo, Hanjin Cui, Pengfei Li, Dandan Feng, En Hu, Qing Huang, Ali Yang, Jun Zhou, Jiekun Luo, Tao Tang, Yang Wang
Lactate accumulation has been observed in the brain with intracerebral hemorrhage (ICH). However, the outcome of lactate accumulation has not been well characterized. Here, we report that lactate accumulation contributes to angiogenesis and neurogenesis in ICH. In the first set of the experiment,  a rat model of ICH was induced by injecting collagenase into the brain. The effects of lactate accumulation on the neurological function, apoptosis, and numbers of newborn endothelial cells and neurons, as well as the proliferation-associated signaling pathway, were evaluated in the rat brain...
July 6, 2018: Experimental & Molecular Medicine
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