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Skeletal Muscle Regeneration

Nancy Lee, Rachel P Spearry, Carolyn E Rydyznski, A John MacLennan
Expression of the ciliary neurotrophic factor (CNTF) receptor essential ligand binding subunit, CNTF receptor α (CNTFRα), is induced in motor neurons and skeletal muscle following peripheral nerve lesion. We previously found muscle CNTFRα promotes motor neuron axon regeneration post-lesion. Both nerve lesion and CNTF administration activate motor neuron signal transducer and activator of transcription 3 (STAT3), a transcription factor implicated in axon growth, suggesting CNTF receptors may contribute to the lesion-induced STAT3 activation...
December 16, 2018: European Journal of Neuroscience
Ju Kyeong Park, Yeon Soo Kim, Sung Un Kang, Yun Sang Lee, Ho-Ryun Won, Chul-Ho Kim
The use of nonthermal atmospheric plasma (NTP) in the biomedical field has recently expanded into cell death induction in cancer, infection prevention, inflammation treatment, and wound-healing enhancement. NTP has been demonstrated to enhance skin and muscle regeneration, but its effects on tissue regeneration, following deep tissue or muscle damage, remains underinvestigated. In this study, we determined the effects of NTP on muscle differentiation and the mechanisms of NTP's contribution to differentiation and regeneration...
December 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Hong Niu, Xiaofei Li, Haichang Li, Zhaobo Fan, Jianjie Ma, Jianjun Guan
Stem cell therapy is a promising approach to regenerate ischemic cardiovascular tissues yet experiences low efficacy. One of the major causes is inferior cell retention in tissues. Injectable cell carriers that can quickly solidify upon injection into tissues so as to immediately increase viscosity have potential to largely improve cell retention. A family of injectable, fast gelling, and thermosensitive hydrogels were developed for delivering stem cells into heart and skeletal muscle tissues. The hydrogels were also photoluminescent with low photobleaching, allowing for non-invasively tracking hydrogel biodistribution and retention by fluorescent imaging...
January 1, 2019: Acta Biomaterialia
Chieh Tseng, Krishna Sinha, Haiying Pan, Yan Cui, Ping Guo, Chih Yi Lin, Fan Yang, Zhenhan Deng, Holger K Eltzschig, Aiping Lu, Johnny Huard
The "super-healing" Murphy Roths Large (MRL/MpJ) mouse possesses a superior regenerative capacity for repair of many tissues, which makes it an excellent animal model for studying molecular and cellular mechanisms during tissue regeneration. Since the role of muscle progenitor cells (MPCs) in muscle healing capacity of MRL/MpJ mice has not been previously studied, we investigated the muscle regenerative capacity of MRL/MpJ mice following muscle injury, and the results were compared to results from C57BL/6 J (B6) age-matched control mice...
December 10, 2018: Stem Cells
Davide Randazzo, Umara Khalique, Joseph J Belanto, Aster Kenea, Dana M Talsness, John T Olthoff, Michelle D Tran, Kristien J Zaal, Katherine Pak, Iago Pinal-Fernandez, Andrew L Mammen, Dan Sackett, James M Ervasti, Evelyn Ralston
In healthy adult skeletal muscle fibers microtubules form a three-dimensional grid-like network. In the mdx mouse, a model of Duchenne muscular dystrophy (DMD), microtubules are mostly disordered, without periodicity. These microtubule defects have been linked to the mdx mouse pathology. We now report that increased expression of the beta 6 class V β-tubulin (tubb6) contributes to the microtubule changes of mdx muscles. Wild-type muscle fibers overexpressing GFP-tubb6 (but not GFP-tubb5) have disorganized microtubules whereas mdx muscle fibers depleted of tubb6 (but not of tubb5) normalize their microtubules, suggesting that increasing tubb6 is toxic...
December 6, 2018: Human Molecular Genetics
Howard D Wang, Denver M Lough, David E Kurlander, Joseph Lopez, Amy Quan, Anand R Kumar
BACKGROUND: Volumetric muscle loss (VML) secondary to traumatic or surgical etiologies can lead to functional and aesthetic impairments. We hypothesize that an implantable muscle-derived stem cell (MDSC) enriched collagen scaffold could significantly augment muscle regeneration in a murine model of VML. METHODS: Murine MDSC were isolated using a modified pre-plating technique and seeded onto type-1 collagen scaffolds to create the MDSC-enriched collagen scaffolds (M-CS)...
November 26, 2018: Plastic and Reconstructive Surgery
Eleonora Guadagnin, Debalina Bagchi, Indranil Sinha, Ronald L Neppl
Skeletal muscle regeneration following injury is a complex multi-stage process involving the recruitment of inflammatory cells, the activation of muscle resident fibroblasts, and the differentiation of activated myoblasts into myocytes. Dysregulation of these cellular processes is associated with ineffective myofiber repair and excessive deposition of extracellular matrix proteins leading to fibrosis. PI3K/Akt signaling is a critical integrator of intra- and intercellular signals connecting nutrient availability to cell survival and growth...
December 7, 2018: Biochemical and Biophysical Research Communications
Baolin Guo, Jin Qu, Xin Zhao, Mengyao Zhang
Injectable conductive hydrogels have great potential as tissue engineering scaffolds and delivery vehicles for electrical signal-sensitive cell therapy. In this work, we present the synthesis of a series of injectable electroactive degradable hydrogels with rapid self-healing ability and their potential application as cell delivery vehicles for skeletal muscle regeneration. Self-healable conductive injectable hydrogels based on dextran-graft-aniline tetramer-graft-4-formylbenzoic acid and N-carboxyethyl chitosan were synthesized under physiological conditions...
December 5, 2018: Acta Biomaterialia
Sung Sup Park, Young-Kyo Seo, Ki-Sun Kwon
The loss of skeletal muscle, called sarcopenia, is an inevitable event during the aging process, and significantly impacts quality of life. Autophagy is known to reduce muscle atrophy caused by dysfunctional organelles, even though the molecular mechanism remains unclear. Here, we have discuss the current understanding of exercise-induced autophagy activation in skeletal muscle regeneration and remodeling, leading to sarcopenia intervention. With aging, dysregulation of autophagy flux inhibits lysosomal storage processes involved in muscle biogenesis...
December 11, 2018: BMB Reports
Hema Bye-A-Jee, Dhamayanthi Pugazhendhi, Samuel Woodhouse, Patrick Brien, Rachel Watson, Martin Turner, Jennifer Pell
BACKGROUND: Members of the ZFP36 family of RNA-binding proteins regulate gene expression post-transcriptionally by binding to AU-rich elements in the 3'UTR of mRNA and stimulating mRNA degradation. The proteins within this family target different transcripts in different tissues. In particular, ZFP36 targets myogenic transcripts and may have a role in adult muscle stem cell quiescence. Our study examined the requirement of ZFP36L1 and ZFP36L2 in adult muscle cell fate regulation. METHODS: We generated single and double conditional knockout mice in which Zfp36l1 and/or Zfp36l2 were deleted in Pax7-expressing cells...
December 7, 2018: Skeletal Muscle
Xiaofan Yang, Pingping Xue, Xin Liu, Xiang Xu, Zhenbing Chen
BACKGROUND: Transforming growth factor beta 1 (TGF-β1) is a classical modulator of skeletal muscle and regulates several processes, such as myogenesis, regeneration and muscle function in skeletal muscle diseases. Skeletal muscle atrophy, characterized by the loss of muscle strength and mass, is one of the pathological conditions regulated by TGF-β1, but the underlying mechanism involved in the atrophic effects of TGF-β1 is not fully understood. METHODS: Mice sciatic nerve transection model was created and gastrocnemius were analysed by western blot, immunofluorescence staining and fibre diameter quantification after 2 weeks...
December 7, 2018: Cell Communication and Signaling: CCS
Naosuke Kamei, Nobuo Adachi, Mitsuo Ochi
Magnetic targeting is a cell delivery system using the magnetic labeling of cells and the magnetic field; it has been developed for minimally invasive cell transplantation. Cell transplantation with both minimal invasiveness and high efficacy on tissue repair can be achieved by this system. Magnetic targeting has been applied for the transplantation of bone marrow mesenchymal stem cells, blood CD133-positive cells, neural progenitor cells, and induced pluripotent stem cells, and for the regeneration of bone, cartilage, skeletal muscles, and the spinal cord...
December 2018: Regenerative Therapy
André V Cordeiro, Vagner R R Silva, José R Pauli, Adelino S R da Silva, Dennys E Cintra, Leandro P Moura, Eduardo R Ropelle
Sphingolipids were discovered more than a century ago and were simply considered as a class of cell membrane lipids for a long time. However, after the discovery of several intracellular functions and their role in the control of many physiological and pathophysiological conditions, these molecules have gained much attention. For instance, the sphingosine-1-phosphate (S1P) is a circulating bioactive sphingolipid capable of triggering strong intracellular reactions through the family of S1P receptors (S1PRs) spread in several cell types and tissues...
December 6, 2018: Journal of Cellular Physiology
Carolyn A Meyers, Joan Casamitjana, Leslie Chang, Lei Zhang, Aaron W James, Bruno Péault
Besides seminal functions in angiogenesis and blood pressure regulation, microvascular pericytes possess a latent tissue regenerative potential that can be revealed in culture following transition into mesenchymal stem cells. Endowed with robust osteogenic potential, pericytes and other related perivascular cells extracted from adipose tissue represent a potent and abundant cell source for refined bone tissue engineering and improved cell therapies of fractures and other bone defects. The use of diverse bone formation assays in vivo, which include mouse muscle pocket osteogenesis and calvaria replenishment, rat and dog spine fusion, and rat non-union fracture healing, has confirmed the superiority of purified perivascular cells for skeletal (re)generation...
2018: Advances in Experimental Medicine and Biology
Miao Zhang, Meng-Zhou Zhang, Shu-Heng Wen, Ying-Fu Sun, Peng-Hao Jiang, Lin-Lin Wang, Rui Zhao, Chang-Liang Wang, Shu-Kun Jiang, Da-Wei Guan
HIPK2 is an evolutionarily conserved serine/threonine kinase and is considered a co-regulator of an increasing number of transcription factors modulating a variety of cellular processes, including inflammation, proliferation and fibrosis. Skeletal muscle injuries repair is an overlapping event between inflammation and tissue repair. There are no reports about HIPK2 expression in skeletal muscles after trauma. A foundational study on distribution and time-dependent expression of HIPK2 was performed by immunohistochemical staining, Western blotting and quantitative real-time PCR, which is expected to obtain a preliminary insight into the functions of HIPK2 during the repair of contused skeletal muscle in mice...
December 5, 2018: Histology and Histopathology
Abdulrahman Jama, Dengtong Huang, Abdullah Ali A Alshudukhi, Roman Chrast, Hongmei Ren
KEY POINTS: Lipin1 is critical for skeletal muscle development. Lipin1 regulates MyoD and MEF2c expression through PKC/HDAC5 mediated pathway. Inhibition of PKCμ activity suppresses myoblast differentiation through inhibiting MyoD and MEF2c expression. ABSTRACT: Our previous characterization of global lipin1-deficient (fld) mice demonstrated that lipin1 played a novel role in skeletal muscle (SM) regeneration. This study using cell type-specific Myf5-cre;Lipin1fl/fl conditional knockout mice (Lipin1Myf5cKO ) shows that lipin1 is a major determinant of SM development...
December 4, 2018: Journal of Physiology
Kuo Zhang, Yuying Zhang, Lijie Gu, Miaomiao Lan, Chuncheng Liu, Meng Wang, Yang Su, Mengxu Ge, Tongtong Wang, Yingying Yu, Chang Liu, Lei Li, Qiuyan Li, Yaofeng Zhao, Zhengquan Yu, Fudi Wang, Ning Li, Qingyong Meng
Satellite cells are crucial for skeletal muscle regeneration, but the molecular mechanisms regulating satellite cells are not entirely understood. Here, we show that the immunoglobulin superfamily containing leucine-rich repeat (Islr), a newly identified marker for mesenchymal stem cells, stabilizes canonical Wnt signaling and promote skeletal muscle regeneration. Loss of Islr delays skeletal muscle regeneration in adult mice. In the absence of Islr, myoblasts fail to develop into mature myotubes due to defective differentiation...
December 3, 2018: Nature Communications
Juan Martin Silva Garcia, Alyssa Panitch, Sarah Calve
Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair. Typically, fibrotic tissue fills the void, which reduces muscle functionality and limb movement. Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML. Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment...
November 30, 2018: Acta Biomaterialia
Klemen Čamernik, Janja Marc, Janja Zupan
The most studied sources of mesenchymal stem/stromal cells (MSCs) are bone marrow and adipose tissue. However skeletal muscle represents an interesting source of diverse subpopulations of MSCs, such as paired box 7 (Pax-7)-positive satellite cells, fibro-/adipogenic progenitors, PW1-positive interstitial cells and others. The specific properties of some of these muscle-derived cells have encouraged the development of cell therapies for muscle regeneration. However, the identity and multilineage potential of the diverse muscle-resident cells should first be evaluated in vitro, followed by in vivo clinical trials to predict their regenerative capacity...
November 30, 2018: Methods in Molecular Biology
Liping Guo, Huanxian Cui, Guiping Zhao, Ranran Liu, Qinghe Li, Maiqing Zheng, Yuming Guo, Jie Wen
BACKGROUND: Skeletal muscle satellite cells (MSC) are crucial for postnatal growth and regeneration of skeletal muscle. An interaction exists between MSC and intramuscular preadipocytes (IMPA). This study is the first to investigate the effects of IMPA on MSC in chickens and unveil the molecular mechanisms by transcriptome analysis. RESULTS: Primary MSC and IMPA were isolated from the pectoralis major muscle of 7-day-old chickens. After both cell types reached confluence, MSC were cultured alone or co-cultured with IMPA for 2 or 4 d...
November 26, 2018: BMC Genomics
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