keyword
https://read.qxmd.com/read/36893861/the-human-small-intestine-contains-two-functionally-distinct-regulatory-t-cell-subsets
#1
JOURNAL ARTICLE
Sudhir Kumar Chauhan, Raquel Bartolomé Casado, Ole J B Landsverk, Hanna Johannessen, Danh Phung, Hogne Røed Nilsen, Frank Sætre, Jørgen Jahnsen, Rune Horneland, Sheraz Yaqub, Einar Martin Aandahl, Knut E A Lundin, Espen S Bækkevold, Frode L Jahnsen
BACKGROUND: Regulatory CD4 T cells (Tregs) in the mice gut are mainly specific for intestinal antigens and play an important role in the suppression of immune responses against harmless dietary antigens and members of the microbiota. However, information about the phenotype and function of Tregs in the human gut is limited. OBJECTIVE: Here, we performed a detailed characterization of Foxp3+ CD4 Tregs in human normal small intestine (SI) as well as from transplanted duodenum and celiac disease lesions...
March 7, 2023: Journal of Allergy and Clinical Immunology
https://read.qxmd.com/read/36426347/cd4-cd25-t-regulatory-cells-in-renal-transplantation
#2
REVIEW
Jason Cheung, Beata Zahorowska, Michael Suranyi, Jeffrey K W Wong, Jason Diep, Stephen T Spicer, Nirupama D Verma, Suzanne J Hodgkinson, Bruce M Hall
The immune response to an allograft activates lymphocytes with the capacity to cause rejection. Activation of CD4+ CD25+ Foxp3+ T regulatory cells (Treg) can down-regulate allograft rejection and can induce immune tolerance to the allograft. Treg represent <10% of peripheral CD4+ T cells and do not markedly increase in tolerant hosts. CD4+ CD25+ Foxp3+ T cells include both resting and activated Treg that can be distinguished by several markers, many of which are also expressed by effector T cells. More detailed characterization of Treg to identify increased activated antigen-specific Treg may allow reduction of non-specific immunosuppression...
2022: Frontiers in Immunology
https://read.qxmd.com/read/35681539/differential-expression-of-cd45ro-and-cd45ra-in-bovine-t-cells
#3
JOURNAL ARTICLE
Anmol Kandel, Lei Li, Akanksha Hada, Zhengguo Xiao
Effective vaccination induces immune memory to protect animals upon pathogen re-encounter. Despite contradictory reports, bovine memory T cells are identified based on two isoforms of CD45, expression of CD45RO plus exclusion of CD45RA. In this report, we contrasted CD45RA/RO expression on circulatory T cells with IFNγ and IL4 expression induced by a conventional method. To our surprise, 20% of cattle from an enclosed herd did not express CD45RO on T cells without any significant difference on CD45RA expression and IFNγ or IL4 induction...
June 4, 2022: Cells
https://read.qxmd.com/read/32650575/sj%C3%A3-gren-s-syndrome-minor-salivary-gland-cd4-memory-t-cells-associate-with-glandular-disease-features-and-have-a-germinal-center-t-follicular-helper-transcriptional-profile
#4
JOURNAL ARTICLE
Michelle L Joachims, Kerry M Leehan, Mikhail G Dozmorov, Constantin Georgescu, Zijian Pan, Christina Lawrence, M Caleb Marlin, Susan Macwana, Astrid Rasmussen, Lida Radfar, David M Lewis, Donald U Stone, Kiely Grundahl, R Hal Scofield, Christopher J Lessard, Jonathan D Wren, Linda F Thompson, Joel M Guthridge, Kathy L Sivils, Jacen S Moore, A Darise Farris
To assess the types of salivary gland (SG) T cells contributing to Sjögren's syndrome (SS), we evaluated SG T cell subtypes for association with disease features and compared the SG CD4+ memory T cell transcriptomes of subjects with either primary SS (pSS) or non-SS sicca (nSS). SG biopsies were evaluated for proportions and absolute numbers of CD4+ and CD8+ T cells. SG memory CD4+ T cells were evaluated for gene expression by microarray. Differentially-expressed genes were identified, and gene set enrichment and pathways analyses were performed...
July 8, 2020: Journal of Clinical Medicine
https://read.qxmd.com/read/31904703/a-classifier-to-predict-viral-control-after-antiretroviral-treatment-interruption-in-chronic-hiv-1-infected-patients
#5
JOURNAL ARTICLE
Csaba FehÉr, Montserrat Plana, Alberto Crespo Guardo, Nuria Climent, Lorna Leal, Ainoa Ugarte, Irene fernÁndez, María Florencia Etcheverry, Josep María Gatell, Sonsoles SÁnchez-Palomino, Felipe GarcÍa, Patrick Aloy
OBJECTIVES: To construct a classifier that predicts the probability of viral control after analytical antiretroviral treatment interruptions (ATI) in HIV research trials. METHODS: Participants of a dendritic cell-based therapeutic vaccine trial (DCV2) constituted the derivation cohort. One of the primary end-points of DCV2 was the drop of viral load (VL) set point after 12 weeks of ATI (delta VL12). We classified cases as "controllers" (delta VL12 >1 log10 copies/mL, n=12) or"non-controllers" (delta VL12 < 0...
January 3, 2020: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://read.qxmd.com/read/31695692/first-universal-newborn-screening-program-for-severe-combined-immunodeficiency-in-europe-two-years-experience-in-catalonia-spain
#6
JOURNAL ARTICLE
Ana Argudo-Ramírez, Andrea Martín-Nalda, Jose L Marín-Soria, Rosa M López-Galera, Sonia Pajares-García, Jose M González de Aledo-Castillo, Mónica Martínez-Gallo, Marina García-Prat, Roger Colobran, Jacques G Riviere, Yania Quintero, Tatiana Collado, Judit García-Villoria, Antonia Ribes, Pere Soler-Palacín
Severe combined immunodeficiency (SCID), the most severe form of T-cell immunodeficiency, can be screened at birth by quantifying T-cell receptor excision circles (TRECs) in dried blood spot (DBS) samples. Early detection of this condition speeds up the establishment of appropriate treatment and increases the patient's life expectancy. Newborn screening for SCID started in January 2017 in Catalonia, the first Spanish and European region to universally include this testing. The results obtained in the first 2 years of experience are evaluated here...
2019: Frontiers in Immunology
https://read.qxmd.com/read/31393160/receptor-occupancy-measurement-of-anti-pd-1-antibody-drugs-in-support-of-clinical-trials
#7
JOURNAL ARTICLE
Jie Fu, Fang Wang, Li-Hou Dong, Mei-Jing Xing, Xue Cheng, Su Wei, Jing-Zhi Xu, Min Han, Kelly Dong, Hai-Feng Song
Aim: The reliable measurement of receptor occupancy (RO) provides informative data for efficacy and safety evaluation. This study aimed to assess factors affecting RO measurement of anti-PD-1 antibodies in clinical studies. Materials & methods: RO performance was assessed using different T-cell activation markers measured by flow cytometry. The validated methodology was then used in support of a clinical study. Results: The optimized active cell population was comprised of CD45RO+ or CD45RA- T cells. The bioanalytical method was validated for inter- and intra-assay precision (coefficient of variation ≤30%) and sample storage stability for 3 days...
July 2019: Bioanalysis
https://read.qxmd.com/read/31358406/quantification-of-a-cell-mediated-immune-response-against-varicella-zoster-virus-by-assessing-responder-cd4-high-memory-cell-proliferation-in-activated-whole-blood-cultures
#8
JOURNAL ARTICLE
Ahmad M Haredy, Michihiro Takei, Shin-Ichi Iwamoto, Masafumi Ohno, Mitsuyo Kosaka, Kazue Hirota, Ritsuko Koketsu, Toshiomi Okuno, Kazuyoshi Ikuta, Koichi Yamanishi, Hirotaka Ebina
BACKGROUND: Herpes zoster (HZ) is caused by reactivation of a latent varicella zoster virus (VZV). The potential to develop HZ increases with age due to waning of memory cell-mediated immunity (CMI), mainly the CD4 response. Therefore, VZV-CD4-memory T cells (CD4-M) count in blood could serve as a barometer for HZ protection. However, direct quantification of these cells is known to be difficult because they are few in number in the blood. We thus developed a method to measure the proliferation level of CD4-M cells responding to VZV antigen in whole blood culture...
August 23, 2019: Vaccine
https://read.qxmd.com/read/31164285/altered-thymic-cd4-t-cell-recovery-after-allogeneic-hematopoietic-stem-cell-transplantation-is-critical-for-nocardiosis
#9
JOURNAL ARTICLE
Xavier Roussel, Etienne Daguindau, Ana Berceanu, Yohan Desbrosses, Philippe Saas, Christophe Ferrand, Estelle Seilles, Fabienne Pouthier, Eric Deconinck, Fabrice Larosa
PURPOSE OF THE STUDY: Nocardia affects immunocompromised human host exhibiting an altered cell-mediated immunity. Infectious risk after allogeneic hematopoietic cell transplantation (AHCT) is significantly correlated to the recovery status of donor-derived immune system, especially CD4+ T-cells reconstitution and thymopoiesis. The purpose of this paper is to highlight a lack of cell-mediated immunity recovery for patients presenting a nocardiosis compared to a control cohort. PATIENTS AND METHODS: This is a case control retrospective monocentric study...
November 2019: Current Research in Translational Medicine
https://read.qxmd.com/read/30559746/atypical-human-effector-memory-cd4-t-cells-with-a-naive-like-phenotype
#10
REVIEW
Nadia Caccamo, Simone A Joosten, Tom H M Ottenhoff, Francesco Dieli
The induction of adaptive immunological memory, mediated by T and B cells, plays an important role in protective immunity to pathogens induced by previous infections or vaccination. Naive CD4+ T cells that have been primed by antigen develop into memory or effector cells, which may be distinguished by their capability to exert a long-term and rapid response upon re-challenge by antigen, to produce distinct cytokines and surface marker expression phenotypes such as CD45RA/RO, CD27, CD62L, and CCR7. Moreover, a distinct lineage of memory T cells populates tissues (tissue-resident memory T cells or TRM cells) which orchestratea the response to pathogens re encountered at tissue sites...
2018: Frontiers in Immunology
https://read.qxmd.com/read/30096879/sanger-and-next-generation-sequencing-approaches-to-evaluate-hiv-1-virus-in-blood-compartments
#11
JOURNAL ARTICLE
Andrea Arias, Pablo López, Raphael Sánchez, Yasuhiro Yamamura, Vanessa Rivera-Amill
The implementation of antiretroviral treatment combined with the monitoring of drug resistance mutations improves the quality of life of HIV-1 positive patients. The drug resistance mutation patterns and viral genotypes are currently analyzed by DNA sequencing of the virus in the plasma of patients. However, the virus compartmentalizes, and different T cell subsets may harbor distinct viral subsets. In this study, we compared the patterns of HIV distribution in cell-free (blood plasma) and cell-associated viruses (peripheral blood mononuclear cells, PBMCs) derived from ART-treated patients by using Sanger sequencing- and Next-Generation sequencing-based HIV assay...
August 9, 2018: International Journal of Environmental Research and Public Health
https://read.qxmd.com/read/29150407/disease-trends-over-time-and-cd4-ccr5-t-cells-expansion-predict-carotid-atherosclerosis-development-in-patients-with-systemic-lupus-erythematosus
#12
JOURNAL ARTICLE
A Baragetti, G A Ramirez, M Magnoni, K Garlaschelli, L Grigore, M Berteotti, I Scotti, E Bozzolo, A Berti, P G Camici, A L Catapano, A A Manfredi, E Ammirati, G D Norata
BACKGROUND AND AIM: Patients with Systemic Lupus Erythematosus (SLE) present increased cardiovascular mortality compared to the general population. Few studies have assessed the long-term development and progression of carotid atherosclerotic plaque in SLE patients. Our aim was to investigate the association of clinical and laboratory markers of disease activity and classical cardiovascular risk factors (CVRF) with carotid atherosclerosis development in SLE patients in a prospective 5-year study...
January 2018: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://read.qxmd.com/read/28194440/transient-antibody-targeting-of-cd45rc-induces-transplant-tolerance-and-potent-antigen-specific-regulatory-t-cells
#13
JOURNAL ARTICLE
Elodie Picarda, Séverine Bézie, Laetitia Boucault, Elodie Autrusseau, Stéphanie Kilens, Dimitri Meistermann, Bernard Martinet, Véronique Daguin, Audrey Donnart, Eric Charpentier, Laurent David, Ignacio Anegon, Carole Guillonneau
Rat and human CD4+ and CD8+ Tregs expressing low levels of CD45RC have strong immunoregulatory properties. We describe here that human CD45 isoforms are nonredundant and identify distinct subsets of cells. We show that CD45RC is not expressed by CD4+ and CD8+ Foxp3+ Tregs, while CD45RA/RB/RO are. Transient administration of a monoclonal antibody (mAb) targeting CD45RC in a rat cardiac allotransplantation model induced transplant tolerance associated with inhibition of allogeneic humoral responses but maintained primary and memory responses against cognate antigens...
February 9, 2017: JCI Insight
https://read.qxmd.com/read/27486885/inherent-low-erk-and-p38-activity-reduce-fas-ligand-expression-and-degranulation-in-t-helper-17-cells-leading-to-activation-induced-cell-death-resistance
#14
JOURNAL ARTICLE
Doureradjou Peroumal, Thiruvaimozhi Abimannan, Ravichandra Tagirasa, Jyothi Ranjan Parida, Santosh Kumar Singh, Prasantha Padhan, Satish Devadas
Activation Induced Cell Death of T helper cells is central to maintaining immune homeostasis and a perturbation often manifests in aberrant T helper cells that is associated with immunopathologies. Significant presence of T cells positive for IL-17A (Th17) and dual positive for IFN-γ/IL-17A (Th1/Th17) in both effector (CD45RA+RO+) and memory (CD45RA-RO+) compartments with differential FasL protein in RA peripheral blood suggested their differential TCR AICD sensitivity. Lowered active caspase-3 in Th17 and Th1/Th17 over Th1 cells confirmed their capability to resist AICD and pointed to early upstream events...
August 23, 2016: Oncotarget
https://read.qxmd.com/read/27358913/single-cell-analysis-of-glandular-t-cell-receptors-in-sj%C3%A3-gren-s-syndrome
#15
JOURNAL ARTICLE
Michelle L Joachims, Kerry M Leehan, Christina Lawrence, Richard C Pelikan, Jacen S Moore, Zijian Pan, Astrid Rasmussen, Lida Radfar, David M Lewis, Kiely M Grundahl, Jennifer A Kelly, Graham B Wiley, Mikhail Shugay, Dmitriy M Chudakov, Christopher J Lessard, Donald U Stone, R Hal Scofield, Courtney G Montgomery, Kathy L Sivils, Linda F Thompson, A Darise Farris
CD4+ T cells predominate in salivary gland (SG) inflammatory lesions in Sjögren's syndrome (SS). However, their antigen specificity, degree of clonal expansion, and relationship to clinical disease features remain unknown. We used multiplex reverse-transcriptase PCR to amplify paired T cell receptor α (TCRα) and β transcripts of single CD4+ CD45RA- T cells from SG and peripheral blood (PB) of 10 individuals with primary SS, 9 of whom shared the HLA DR3/DQ2 risk haplotype. TCRα and β sequences were obtained from a median of 91 SG and 107 PB cells per subject...
June 2, 2016: JCI Insight
https://read.qxmd.com/read/27100180/cd45-isoform-profile-identifies-natural-killer-nk-subsets-with-differential-activity
#16
JOURNAL ARTICLE
Ewelina Krzywinska, Amelie Cornillon, Nerea Allende-Vega, Dang-Nghiem Vo, Celine Rene, Zhao-Yang Lu, Christine Pasero, Daniel Olive, Nathalie Fegueux, Patrick Ceballos, Yosr Hicheri, Michal Sobecki, Jean-François Rossi, Guillaume Cartron, Martin Villalba
The leucocyte-specific phosphatase CD45 is present in two main isoforms: the large CD45RA and the short CD45RO. We have recently shown that distinctive expression of these isoforms distinguishes natural killer (NK) populations. For example, co-expression of both isoforms identifies in vivo the anti tumor NK cells in hematological cancer patients. Here we show that low CD45 expression associates with less mature, CD56bright, NK cells. Most NK cells in healthy human donors are CD45RA+CD45RO-. The CD45RA-RO+ phenotype, CD45RO cells, is extremely uncommon in B or NK cells, in contrast to T cells...
2016: PloS One
https://read.qxmd.com/read/26696529/differences-in-t-cell-distribution-and-ccr5-expression-in-hiv-positive-and-hiv-exposed-seronegative-persons-who-inject-drugs
#17
JOURNAL ARTICLE
Eveli Kallas, Kristi Huik, Silver Türk, Merit Pauskar, Ene-Ly Jõgeda, Marina Šunina, Tõnis Karki, Don Des Jarlais, Anneli Uusküla, Radko Avi, Irja Lutsar
Some individuals remain uninfected despite repeated exposure to HIV. This protection against HIV has been partly associated with altered T cell subset distributions and CCR5 expression levels. However, the majority of studies have been conducted in sexually exposed subjects. We aimed to assess whether HIV infection and intravenous drug use were associated with differences in CCR5 expression, immune activation on the CD4+ and CD8+ T cells and T cell distribution among Caucasian persons who inject drugs (PWIDs)...
June 2016: Medical Microbiology and Immunology
https://read.qxmd.com/read/26629531/identification-of-anti-tumor-cells-carrying-natural-killer-nk-cell-antigens-in-patients-with-hematological-cancers
#18
JOURNAL ARTICLE
Ewelina Krzywinska, Nerea Allende-Vega, Amelie Cornillon, Dang-Nghiem Vo, Laure Cayrefourcq, Catherine Panabieres, Carlos Vilches, Julie Déchanet-Merville, Yosr Hicheri, Jean-François Rossi, Guillaume Cartron, Martin Villalba
Natural killer (NK) cells, a cytotoxic lymphocyte lineage, are able to kill tumor cells in vitro and in mouse models. However, whether these cells display an anti-tumor activity in cancer patients has not been demonstrated. Here we have addressed this issue in patients with several hematological cancers. We found a population of highly activated CD56(dim)CD16(+) NK cells that have recently degranulated, evidence of killing activity, and it is absent in healthy donors. A high percentage of these cells expressed natural killer cell p46-related protein (NKp46), natural-killer group 2, member D (NKG2D) and killer inhibitory receptors (KIRs) and a low percentage expressed NKG2A and CD94...
October 2015: EBioMedicine
https://read.qxmd.com/read/24875455/diagnosis-and-management-of-nocardiosis-after-bone-marrow-stem-cell-transplantation-in-adults-lack-of-lymphocyte-recovery-as-a-major-contributing-factor
#19
JOURNAL ARTICLE
L Mansi, E Daguindau, P Saas, F Pouthier, C Ferrand, A Dormoy, I Patry, F Garnache, P-S Rohrlich, E Deconinck, F Larosa
Hematopoietic cell transplantation (HCT) is a curative treatment for hematological malignancies. This therapeutic approach is associated with a profound immune deficiency and an increased rate of opportunistic infections. Nocardiosis is a rare bacterial infection occurring mainly in patients with deficient cell-mediated immunity, such as AIDS patients or transplant recipients. Diagnosis of nocardiosis can be challenging, as signs and symptoms are non-specific. Routine prophylaxis with trimethoprin/sulfamethoxazole (TMP/SMZ) does not prevent the risk of infection...
June 2014: Pathologie-biologie
https://read.qxmd.com/read/24760139/molecular-signatures-differentiate-immune-states-in-type-1-diabetic-families
#20
JOURNAL ARTICLE
Yi-Guang Chen, Susanne M Cabrera, Shuang Jia, Mary L Kaldunski, Joanna Kramer, Sami Cheong, Rhonda Geoffrey, Mark F Roethle, Jeffrey E Woodliff, Carla J Greenbaum, Xujing Wang, Martin J Hessner
Mechanisms associated with type 1 diabetes (T1D) development remain incompletely defined. Using a sensitive array-based bioassay where patient plasma is used to induce transcriptional responses in healthy leukocytes, we previously reported disease-specific, partially interleukin (IL)-1-dependent signatures associated with preonset and recent onset (RO) T1D relative to unrelated healthy control subjects (uHC). To better understand inherited susceptibility in T1D families, we conducted cross-sectional and longitudinal analyses of healthy autoantibody-negative (AA(-)) high HLA-risk siblings (HRS) (DR3 and/or DR4) and AA(-) low HLA-risk siblings (LRS) (non-DR3/non-DR4)...
November 2014: Diabetes
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