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https://www.readbyqxmd.com/read/30101757/an-excitation-emission-fluorescence-lifetime-spectrometer-eefls-using-a-frequency-doubled-supercontinuum-laser-source
#1
Dzmitry Melnikau, Saioa Elcoroaristizabal, Alan George Ryder
The accurate fluorescence analysis of complex, multi-fluorophore containing proteins requires the use of multi-dimensional measurement techniques. For the measurement of intrinsic fluorescence from tyrosine (Tyr) and tryptophan (Trp) one needs tunable UV excitation and for steady-state measurements like Excitation Emission Matrix (EEM) simple pulsed Xe lamps are commonly used. Unfortunately, simultaneous multi-dimensional wavelength and time resolved measurement of intrinsic protein fluorescence in the 260 to 400 nm spectral range are challenging and typically required the use of very complex tunable laser systems or multiple single excitation wavelength sources...
August 13, 2018: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/30100106/preserved-anabolic-threshold-and-capacity-as-estimated-by-a-novel-stable-tracer-approach-suggests-no-anabolic-resistance-or-increased-requirements-in-weight-stable-copd-patients
#2
Renate Jonker, Nicolaas E P Deutz, Gerdien C Ligthart-Melis, Anthony J Zachria, Eugene A Veley, Rajesh Harrykissoon, Mariëlle P K J Engelen
BACKGROUND & AIMS: Assessing the ability to respond anabolic to dietary protein intake during illness provides important insight in the capacity of lean body mass maintenance. We applied a newly developed stable tracer approach to assess in one session in patients with chronic obstructive pulmonary disease (COPD) and healthy older adults both the minimal amount of protein intake to obtain protein anabolism (anabolic threshold) and the efficiency of dietary protein to promote protein anabolism (anabolic capacity)...
July 31, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/30076556/patient-specific-image-based-bone-marrow-dosimetry-in-lu-177-dota-0-tyr-3-octreotate-and-lu-177-dkfz-psma-617-therapy-investigation-of-a-new-hybrid-image-approach
#3
Astrid Gosewisch, Andreas Delker, Sebastian Tattenberg, Harun Ilhan, Andrei Todica, Julia Brosch, Lena Vomacka, Anika Brunegraf, Franz Josef Gildehaus, Sibylle Ziegler, Peter Bartenstein, Guido Böning
BACKGROUND: The bone marrow (BM) is a main organ at risk in Lu-177-PSMA-617 therapy of prostate cancer and Lu-177-Octreotate therapy of neuroendocrine tumours. BM dosimetry is challenging and time-consuming, as different sequential quantitative measurements must be combined. The BM absorbed dose from the remainder of the body (ROB) can be determined from sequential whole-body planar (WB-P) imaging, while quantitative Lu-177-SPECT allows for more robust tumour and organ absorbed doses...
August 3, 2018: EJNMMI Research
https://www.readbyqxmd.com/read/30057257/moderation-of-the-relationship-between-toxoplasma-gondii-seropositivity-and-trait-impulsivity-in-younger-men-by-the-phenylalanine-tyrosine-ratio
#4
Xiaoqing Peng, Lisa A Brenner, Ashwin J Mathai, Thomas B Cook, Dietmar Fuchs, Nadine Postolache, Maureen W Groer, Janardan P Pandey, Farooq Mohyuddin, Ina Giegling, Abhishek Wadhawan, Annette M Hartmann, Bettina Konte, Lena Brundin, Marion Friedl, John W Stiller, Christopher A Lowry, Dan Rujescu, Teodor T Postolache
Previously, we reported that Toxoplasma gondii (T. gondii)-seropositivity is associated with higher impulsive sensation seeking in younger men. As dopaminergic and serotonergic signaling regulate impulsivity, and as T. gondii directly and indirectly affects dopaminergic signaling and induces activation of the kynurenine pathway leading to the diversion of tryptophan from serotonin production, we investigated if dopamine and serotonin precursors or the tryptophan metabolite kynurenine interact with the T. gondii-impulsivity association...
March 22, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/30043079/plasma-profile-and-urine-excretion-of-amino-acids-in-children-with-celiac-disease-on-gluten-free-diet-after-oligofructose-enriched-inulin-intervention-results-of-a-randomised-placebo-controlled-pilot-study
#5
Natalia Drabińska, Urszula Krupa-Kozak, Ewa Ciska, Elżbieta Jarocka-Cyrta
The circulating amino acid (AAs) concentrations are indicators of dietary protein intake and metabolic status. In celiac disease (CD), the AA imbalance is frequently observed. Prebiotics are found to alleviate nutrient deficiencies. Therefore, the aim of this study was to analyse the impact of oligrofructose-enriched inulin (Synergy 1), administered for 3 months as a gluten-free diet (GFD) supplement to children with CD, on the plasma and urine concentrations of AAs. CD children (N = 34) were randomised into two groups, receiving Synergy 1 (10 g/day) or placebo (maltodextrin) for 3 months...
July 24, 2018: Amino Acids
https://www.readbyqxmd.com/read/30033019/molecular-characterization-of-%C3%AE-endoglucanase-from-antagonistic-trichoderma-saturnisporum-isolate-gitx-panog-c-induced-under-mycoparasitic-conditions
#6
Vivek Sharma, Richa Salwan, V Shanmugam
The endoglucanase belonging to glycoside hydrolase family 61 are little studied. In present study, a β-endoglucanase of ~37 kDa induced on autoclaved mycelium of Fusarium oxysporum was cloned and characterized. The molecular characterization of β-endoglucanase encoding gene revealed presence of a single intron and an open reading frame of 1044-bp which encoded a protein of 347 amino acid residues. The phylogenetic analysis of Eglu revealed its similarity to endo-β-glucanases of other Trichoderma spp. The catalytic site of β-endoglucanase contained Asp, Asn, His and Tyr residues...
July 2018: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/30023297/salivary-serotonin-does-not-correlate-with-central-serotonin-turnover-in-adult-phenylketonuria-pku-patients
#7
Joseph Leung, Caroline Selvage, Taryn Bosdet, Jennifer Branov, Annie Rosen-Heath, Carole Bishop, Sandra Sirrs, Gabriella Horvath
Introduction: Phenylketonuria (PKU) is an inborn error of metabolism associated with an increased risk of behavioural and mood disorders. There are currently no reliable markers for monitoring mood in PKU. The purpose of this study was to evaluate salivary serotonin as a possible non-invasive marker of long-term mood symptoms and central serotonin activity in patients with PKU. Methods: 20 patients were recruited from our Adult Metabolic Diseases Clinic. Age, sex, plasma phenylalanine (Phe) level, DASS (Depression Anxiety Stress Scales) depression score, DASS anxiety score, BMI, salivary serotonin, salivary cortisol, 2-year average Phe, 2-year average tyrosine (Tyr), and 2-year average Phe:Tyr ratio were collected for each patient...
June 2018: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/30010851/magnetic-resonance-imaging-surrogates-of-molecular-subgroups-in-atypical-teratoid-rhabdoid-tumor-atrt
#8
Johannes Nowak, Karolina Nemes, Annika Hohm, Lindsey A Vandergrift, Martin Hasselblatt, Pascal D Johann, Marcel Kool, Michael C Frühwald, Monika Warmuth-Metz
Background: Recently, three molecular subgroups of atypical teratoid/rhabdoid tumor (ATRT) were identified, but little is known on their clinical and magnetic resonance imaging (MRI) characteristics. Methods: A total of 43 patients with known molecular subgroup status (ATRT-SHH n=17, ATRT-TYR n=16, ATRT-MYC n=10) were retrieved from the EU-RHAB registry and analyzed for clinical and MRI features. Results: On MRI review, differences in preferential tumor location were confirmed, with ATRT-TYR being predominantly located infratentorially (p<...
July 13, 2018: Neuro-oncology
https://www.readbyqxmd.com/read/30003472/amino-acid-supplementations-enhance-the-stress-resistance-and-fermentation-performance-of-lager-yeast-during-high-gravity-fermentation
#9
Hongjie Lei, Li Feng, Fei Peng, Huaide Xu
The effects of different wort gravity or ethanol concentration in initial wort on the fermentation performance of lager yeast and assimilation of free amino acids (FAAs) were studied. Results showed that compared with high wort gravity (24°P), high ethanol concentration (10%, v/v) decreased yeast growth, cell viability, and wort fermentability significantly. The assimilation of FAAs was changed dramatically by high ethanol toxicity, and positive correlations between the assimilation amounts of 10 FAAs (Asp, Ser, Gly, Arg, Tyr, Val, Met, Lys, Ile, and Leu) and fermentation performance (cell viability, fermentability, and ethanol production) were identified, especially for Arg and Lys exhibiting extremely significant positive correlations...
July 13, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/30002107/targeting-of-the-cholecystokinin-2-receptor-with-the-minigastrin-analog-177-lu-dota-pp-f11n-does-the-use-of-protease-inhibitors-further-improve-in-vivo-distribution
#10
Alexander Walter Sauter, Rosalba Mansi, Ulrich Hassiepen, Lionel Muller, Tania Panigada, Stefan Wiehr, Anna-Maria Wild, Susanne Geistlich, Martin Béhé, Christof Rottenburger, Damian Wild, Melpomeni Fani
Patients with metastatic medullary thyroid cancer (MTC) have limited systemic treatment options. The use of radiolabeled gastrin analogs targeting the cholecystokinin-2 receptor (CCK2R) is an attractive approach. However, their therapeutic efficacy is presumably decreased by their enzymatic degradation in vivo. We aimed to investigate whether the chemically stabilized analog 177 Lu-DOTA-PP-F11N (177 Lu-DOTA-(DGlu)6-Ala-Tyr-Gly-Trp-Nle-Asp-Phe-NH2) performs better than reference analogs with varying in vivo stability, namely 177 Lu-DOTA-MG11 (177 Lu-DOTA-DGlu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2) and 177 Lu-DOTA-PP-F11 (177 Lu-DOTA-(DGlu)6-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2), and if the use of protease inhibitors further improves CCKR2-targeting...
July 12, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29981683/veronica-persica-poir-extract-antibacterial-antifungal-and-scolicidal-activities-and-inhibitory-potential-on-acetylcholinesterase-tyrosinase-lipoxygenase-and-xanthine-oxidase
#11
Javad Sharifi-Rad, Ghazaleh S Tayeboon, Fereshteh Niknam, Majid Sharifi-Rad, Maryam Mohajeri, Bahare Salehi, Marcello Iriti, Mehdi Sharifi-Rad
Veronica persica (Persian speedwell) is a flowering plant local to Eurasia. In this study, several analyses were done to discover the antimicrobial and scolicidal activities and acetyl cholinesterase (AChE), tyrosinase (TYR), lipoxygenase (LOX), and xanthine oxidase (XO) inhibitory activities of V. persica extract. The results presented that B. subtilis was the most susceptible to the extract (MIC = 40.3 μg/mL), while P. aeruginosa was the most resistant strain (MIC = 250.9 μg/mL) among all bacteria evaluated...
June 25, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29979040/functional-evaluation-of-the-%C3%AF-helix-in-the-nad-p-h-fmn-reductase-of-the-alkanesulfonate-monooxygenase-system
#12
Jonathan M Musila, Dianna L Forbes, Holly R Ellis
A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 helix. The Tyr insertional residue of SsuE makes specific contacts across the dimer interface that may assist in the altered mechanistic properties of this enzyme. The Y118F SsuE variant maintained the π-π stacking interactions at the tetramer interface and had kinetic parameters similar to those of wild-type SsuE...
July 31, 2018: Biochemistry
https://www.readbyqxmd.com/read/29976562/an-outward-facing-aromatic-amino-acid-is-crucial-for-signaling-between-the-membrane-spanning-and-nucleotide-binding-domains-of-multidrug-resistance-protein-1-mrp1-abcc1
#13
Kevin E Weigl, Gwenaëlle Conseil, Alice J Rothnie, May Arama, Yossi Tsfadia, Susan P C Cole
The 190-kDa human MRP1 is an ATP-binding cassette multidrug and multiorganic anion efflux transporter. The 17 transmembrane helices of its three membrane-spanning domains, together with its two nucleotide binding domains (NBDs), form a stabilizing network of domain-domain interactions that ensure substrate binding in the cytoplasm is efficiently coupled to ATP binding and hydrolysis to effect solute efflux into the extracellular milieu. Here we show that Ala substitution of Phe583 in an outward-facing loop between the two halves of the transporter essentially eliminates the binding of multiple organic anions by MRP1...
September 2018: Molecular Pharmacology
https://www.readbyqxmd.com/read/29974532/genetic-variants-associated-with-skin-photosensitivity-in-a-southern-european-population-from-spain
#14
Barbara Hernando, Elena Sanz-Page, Gerard Pitarch, Laura Mahiques, Francisca Valcuende-Cavero, Conrado Martinez-Cadenas
BACKGROUND/PURPOSE: Recent GWAS studies, mostly performed in populations of North European origin, have identified the genetic loci associated with pigmentation, sun sensitivity, freckling and skin cancer susceptibility. Here, we aimed at addressing the genetic determinants of sunlight sensitivity in Spain, a southern European population. METHODS: Nine SNPs located in 8 pigmentation-related genes (IRF4, TYR, ASP, HERC2, OCA2, BNC2, SLC24A4 and SLC45A2) were genotyped in 456 Spaniards...
July 5, 2018: Photodermatology, Photoimmunology & Photomedicine
https://www.readbyqxmd.com/read/29953888/the-dual-role-of-the-2-oh-group-of-a76-trna-tyr-in-the-prevention-of-d-tyrosine-mistranslation
#15
Mariia Yu Rybak, Oksana P Kovalenko, Michael A Tukalo
Aminoacyl-tRNA-synthetases are crucial enzymes for initiation step of translation. Possessing editing activity, they protect living cells from misincorporation of non-cognate and non-proteinogenic amino acids into proteins. Tyrosyl-tRNA synthetase (TyrRS) does not have such editing properties, but it shares weak stereospecificity in recognition of d-/l-tyrosine (Tyr). Nevertheless, an additional enzyme, d-aminoacyl-tRNA-deacylase (DTD), exists to overcome these deficiencies. The precise catalytic role of hydroxyl groups of the tRNATyr A76 in the catalysis by TyrRS and DTD remained unknown...
August 17, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29948654/the-influence-of-blood-phenylalanine-levels-on-neurocognitive-function-in-adult-pku-patients
#16
A Bartus, F Palasti, E Juhasz, E Kiss, E Simonova, Cs Sumanszki, P Reismann
It is well known that hyperphenylalaninemia caused by phenylketonuria (PKU) negatively influences cognitive performance. Several tests have been used to study these functions. Until now, no universal, optimal tool has been developed for detecting PKU-caused brain dysfunctions. Using computerized neuropsychological tests during daily routine would be helpful for screening subclinical brain deficits in adult PKU patients. In a monocentric, cross-sectional study, adult patients with PKU (n = 46; median age = 29...
June 12, 2018: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29942080/cleavage-of-a-carbon-fluorine-bond-by-an-engineered-cysteine-dioxygenase
#17
Jiasong Li, Wendell P Griffith, Ian Davis, Inchul Shin, Jiangyun Wang, Fahui Li, Yifan Wang, Daniel J Wherritt, Aimin Liu
Cysteine dioxygenase (CDO) plays an essential role in sulfur metabolism by regulating homeostatic levels of cysteine. Human CDO contains a post-translationally generated Cys93-Tyr157 cross-linked cofactor. Here, we investigated this Cys-Tyr cross-linking by incorporating unnatural tyrosines in place of Tyr157 via a genetic method. The catalytically active variants were obtained with a thioether bond between Cys93 and the halogen-substituted Tyr157, and we determined the crystal structures of both wild-type and engineered CDO variants in the purely uncross-linked form and with a mature cofactor...
June 25, 2018: Nature Chemical Biology
https://www.readbyqxmd.com/read/29933512/protein-kinase-d-displays-intrinsic-tyr-autophosphorylation-activity-insights-into-mechanism-and-regulation
#18
Mathias Cobbaut, Rita Derua, Peter J Parker, Etienne Waelkens, Veerle Janssens, Johan Van Lint
The protein kinase D (PKD) family is regulated through multi-site phosphorylation, including autophosphorylation. For example, PKD displays in vivo autophosphorylation on Ser-742 (and Ser-738 in vitro) in the activation loop and Ser-910 in the C-tail (hPKD1 numbering). In this paper, we describe the surprising observation that PKD also displays in vitro autocatalytic activity towards a Tyr residue in the P + 1 loop of the activation segment. We define the molecular determinants for this unusual activity and identify a Cys residue (C705 in PKD1) in the catalytic loop as of utmost importance...
June 22, 2018: FEBS Letters
https://www.readbyqxmd.com/read/29910687/atg7-inhibits-warburg-effect-by-suppressing-pkm2-phosphorylation-resulting-reduced-epithelial-mesenchymal-transition
#19
Yanling Feng, Jingwei Liu, Wendong Guo, Yi Guan, Hongde Xu, Qiqiang Guo, Xiaoyu Song, Fei Yi, Ting Liu, Wenyu Zhang, Xiang Dong, Longyue L Cao, Brian P O'Rourke, Liu Cao
Metabolic reprogramming is a distinct hallmark in tumorigenesis. Autophagy can rewire cell metabolism by regulating intracellular homeostasis. Warburg effect is a specific energy metabolic process that allows tumor cells to metabolize glucose via glycolysis into lactate even in the presence of oxygen. Although both autophagy and Warburg effect are involved in the stress response to energy crisis in tumor cells, their molecular relationship has remained largely elusive. We found that Atg7, a key molecule involved in autophagy, inhibits the Warburg effect...
2018: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29899551/proscillaridin-a-induces-apoptosis-and-suppresses-non-small-cell-lung-cancer-tumor-growth-via-calcium-induced-dr4-upregulation
#20
Run-Ze Li, Xing-Xing Fan, Fu-Gang Duan, Ze-Bo Jiang, Hu-Dan Pan, Lian-Xiang Luo, Yan-Ling Zhou, Ying Li, Ying-Jia Yao, Xiao-Jun Yao, Elaine Lai-Han Leung, Liang Liu
Non-small-cell lung cancer (NSCLC) is the predominant histological type of lung cancer and is characterized by the highest mortality and incidence rates among these types of malignancies. Cardiac glycosides, a class of natural products, have been identified as a potential type of chemotherapeutic agent. This study aims to investigate the anti-cancer effects and the mechanisms of action of Proscillaridin A (P.A) in NSCLC cells. In vitro sodium-potassium pump (Na+ /K+ ATPase) enzyme assays indicated that P.A is a direct Na+ /K+ ATPase inhibitor...
June 13, 2018: Cell Death & Disease
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