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Tyr p

Meihua Gong, Pan Zhang, Chunyang Li, Xu Ma, Daping Yang
BACKGROUND/AIMS: Skin photoaging is primarily caused by the functional attrition of skin stem cells. The skin stem cell niche plays an important role in maintaining stem cell survival and behaviour. In our study, we hypothesized that UVB irradiation induces skin photoaging by changing skin stem cell niches and that transferred adipose-derived stem cells (ADSCs) can remodel the niches by affecting the BMP signalling pathway and transdifferentiating into skin stem cells. METHODS: Sixty-four C57BL/6J mice were divided into the following groups: a control group, the UVB group and the UVB+ADSCs group...
2018: Cellular Physiology and Biochemistry
Maximilian Klingler, Dominik Summer, Christine Rangger, Roland Haubner, Julie Foster, Jane Sosabowski, Irene Virgolini, Clemens Decristoforo, Elisabeth von Guggenberg
Molecular imaging and targeted radiotherapy (TRT) with radiolabeled cholecystokinin-2-receptor (CCK2R) targeting peptide probes holds high promise to improve the clinical management of patients with metastatic medullary thyroid carcinoma (MTC) and other CCK2R-expressing malignancies. Low stability and suboptimal targeting of currently available radiolabeled peptide analogs has prompted us to seek for new stabilization strategies. In this study we present a new minigastrin analog with site-specific C-terminal modifications showing a highly optimized targeting profile...
December 7, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Yuanjun Deng, Yang Cai, Lele Liu, Xueping Lin, Pingfan Lu, Yiyan Guo, Min Han, Gang Xu
Protein tyrosine (Tyr) nitration, the covalent addition of a nitro group (• NO2 ) to Tyr residues, is emerging as a candidate mechanism of endothelial dysfunction. Previous studies have shown that Tyr nitration is primarily induced by nitrosative stress, a process characterized by the production of reactive nitrogen species, especially peroxynitrite anion (ONOO- ), which is considered a secondary product of NO in the presence of superoxide radicals (O2 •- ). However, the impact of nitrosative stress-induced Tyr nitration on endothelial dysfunction has not been thoroughly elucidated to date...
December 6, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Hui-Hua Ge, Yue Qiu, Zhi-Wei Yi, Run-Ying Zeng, Guang-Ya Zhang
Acidic xylanases possess the unique features necessary for the tolerance of acidic environments, which may have great potentials for industrial purposes. However, factors controlling the pH-dependent stability of xylanases are only partially known. Here we proposed a residue interaction networks based method to analyze the differences of residue interactions between 6 pairs of experimentally verified acidic and neutral xylanases. They had very close numbers of aromatic amino acids, however extremely significant more (p < 0...
December 2, 2018: International Journal of Biological Macromolecules
Alicia DiBattista, Nathan McIntosh, Monica Lamoureux, Osama Y Al-Dirbashi, Pranesh Chakraborty, Philip Britz-McKibbin
Cystic fibrosis (CF) is a complex multi-organ disorder that is among the most common fatal genetic diseases benefiting from therapeutic interventions early in life. Newborn screening (NBS) for pre-symptomatic detection of CF currently relies on a two-stage immunoreactive trypsinogen (IRT) and cystic fibrosis transmembrane conductance regulator (CFTR) mutation panel algorithm that is sensitive, but not specific for identifying affected neonates with a low positive predictive value. For the first time, we report the discovery of a panel of CF-specific metabolites from a single 3...
December 3, 2018: Journal of Proteome Research
Xiaoming Zhou, Fangfei Yang, Qin Zhang, Yuan Miao, Xuejun Hu, Ailin Li, Gang Hou, Qiuyue Wang, Jian Kang
Background: Family with sequence similarity 129, member B ( FAM129B ), also called MINERVA, is upregulated and promotes tumor invasion in multiple types of cancer. However, the mechanism and clinicopathological significance of FAM129B remains unclear. Materials and methods: Online KM-plotter tool and immunohistochemistry were used to predict the prognostic value of FAM129B expression in lung cancer tissues. Western blotting analysis, MTT, colony formation assay and matrigel invasion assay were performed after overexpressing or depleting FAM129B ...
2018: OncoTargets and Therapy
Natalie Landry, Morvarid S Kavosh, Krista L Filomeno, Sunil G Rattan, Michael P Czubryt, Ian M C Dixon
Many etiologies of heart disease are characterized by expansion and remodeling of the cardiac extracellular matrix (ECM or matrix) which results in cardiac fibrosis. Cardiac fibrosis is mediated in cardiac fibroblasts by TGF-β1 /R-Smad2/3 signaling. Matrix component proteins are synthesized by activated resident cardiac fibroblasts known as myofibroblasts (MFB). These events are causal to heart failure with diastolic dysfunction and reduced cardiac filling. We have shown that exogenous Ski, a TGF-β1 /Smad repressor, localizes in the cellular nucleus and deactivates cardiac myofibroblasts...
November 2018: Physiological Reports
Huadong Liu, Haiming Huang, Courtney Voss, Tomonori Kaneko, Wentao Qin, Sachdev Sidhu, Shawn S-C Shun-Cheng Li
Src homology 2 (SH2) domains play an essential role in cellular signal transduction by binding to proteins phosphorylated on Tyr residue. Although Tyr phosphorylation (pY) is a prerequisite for binding for essentially all SH2 domains characterized to date, different SH2 domains prefer specific sequence motifs C-terminal to the pY residue. Because all SH2 domains adopt the same structural fold, it is not well understood how different SH2 domains have acquired the ability to recognize distinct sequence motifs...
November 27, 2018: Molecular & Cellular Proteomics: MCP
Stefan A P Lenz, Stacey D Wetmore
Parasitic protozoa rely on nucleoside hydrolases that play key roles in the purine salvage pathway by catalyzing the hydrolytic cleavage of the N-glycosidic bond that connects nucleobases to ribose sugars. Cytidine-uridine nucleoside hydrolase (CU-NH) is generally specific toward pyrimidine nucleosides; however, previous work has shown that replacing two active site residues with Tyr, specifically the Thr223Tyr and Gln227Tyr mutations, allows CU-NH to process inosine. The current study uses molecular dynamics (MD) simulations to gain atomic-level insight into the activity of wild-type and mutant E...
November 26, 2018: Journal of Computer-aided Molecular Design
Solene Monfermé, Eulalie Lasseaux, Catherine Duncombe-Poulet, Christian Hamel, Sabine Defoort-Dhellemmes, Isabelle Drumare, Xavier Zanlonghi, Hélène Dollfus, Yaurama Perdomo, Dominique Bonneau, Jean-François Korobelnik, Claudio Plaisant, Vincent Michaud, Perrine Pennamen, Caroline Rooryck-Thambo, Fanny Morice-Picard, Clement Paya, Benoit Arveiler
AIM: Oculocutaneous albinism type 1 (OCA1) is due to TYR mutations. c.1205G>A/p.Arg402Gln (R402Q) is a thermosensitive variant of the TYR gene that has been reported to be responsible for mild forms of OCA1. The aim of our study was to define the phenotype associated with this variant. METHODS: In our retrospective series, among 268 patients diagnosed with OCA1, 122 (45.5%) harboured one pathogenic variant of TYR , and the R402Q variant ensured to be in trans by segregation analysis in 69 patients (25...
November 24, 2018: British Journal of Ophthalmology
Feifei Yu, Bingliang Qu, Dandan Lin, Yuewen Deng, Ronglian Huang, Zhiming Zhong
The paired-box 3 ( Pax3 ) is a transcription factor and it plays an important part in melanin synthesis. In this study, a new Pax3 gene was identified from Pteria penguin ( Röding , 1798 ) ( P. penguin ) by RACE-PCR (rapid-amplification of cDNA ends-polymerase chain reaction) and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of PpPax3 was 2250 bp long, containing an open reading fragment of 1365 bp encoding 455 amino acids. Amino acid alignment and phylogenetic tree showed PpPax3 shared the highest (69...
November 22, 2018: International Journal of Molecular Sciences
Patricia Sanmartín-Salinas, Luis G Guijarro
We reported that insulin receptor substrate 4 (IRS-4) levels increased in tissue from colorectal cancer (CRC) patients and promoted retinoblastoma-cyclin-dependent kinase activation. The aim of the present study was to evaluate the effect of IRS-4 on IGF-1 receptor pathway and its impact on procaspase 3 and PARP expression in RKO and HepG2 cancer cell lines. The results obtained in vitro were compared with those obtained from biopsies of patients with CRC (n = 18), tubulovillous adenomas (TA) (n = 2) and in matched adjacent normal colorectal (MANC) tissue (n = 20)...
2018: Journal of Oncology
Rudolf A Werner, Heribert Hänscheid, Jeffrey P Leal, Mehrbod S Javadi, Takahiro Higuchi, Martin A Lodge, Andreas K Buck, Martin G Pomper, Constantin Lapa, Steven P Rowe
PURPOSE: As has been previously reported, the somatostatin receptor (SSTR) imaging agent [68 Ga]-labeled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetraacetic acid-d-Phe(1)-Tyr(3)-octreotate ([68 Ga]DOTATATE) demonstrates lower uptake in normal organs in patients with a high neuroendocrine tumor (NET) burden. Given the higher SSTR affinity of [68 Ga] DOTATATE, we aimed to quantitatively investigate the biodistribution of [68 Ga]-labeled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetraacetic acid-d-Phe(1)-Tyr(3)-octreotide ([68 Ga]DOTATOC) to determine a potential correlation between uptake in normal organs and NET burden...
November 7, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Marzena Tylicka, Ewa Matuszczak, Maria Karpińska, Adam Hermanowicz, Wojciech Dębek, Halina Ostrowska
Surgical tissue damage and the accompanying inflammatory response lead to proteasome activation, initiation of damaged protein degradation, and induction of acute-phase inflammatory response. The aim of this study was to investigate the rate of change in proteasome chymotrypsin-like (ChT-L) activity and C-reactive protein concentration depending on the degree of tissue damage and their correlation with prealbumin concentrations in children before and after abdominal surgery. This experimental study included children who underwent abdominal surgery between 2015 and 2017...
2018: Mediators of Inflammation
Carolina Castaño, Mariana P Serrano, Carolina Lorente, Claudio D Borsarelli, Andrés H Thomas
Unconjugated oxidized pterins accumulate in the skin of patients suffering from vitiligo and, under UV-A irradiation, photosensitize the oxidation of amino acids. In this work we study the interaction of the singlet and triplet excited states of pterin (Ptr), the parent compound of oxidized pterins, with four oxidizable amino acids: tryptophan (Trp), tyrosine (Tyr), histidine (His) and methionine (Met). Steady-state and time-resolved fluorescence measurements and laser flash photolysis experiments were performed to investigate the quenching of the Ptr excited states by the amino acids in aqueous solution...
November 7, 2018: Photochemistry and Photobiology
Xue-Ying Wang, Hui-Ru Zhou, Sha Wang, Chao-Yang Liu, Guang-Cheng Qin, Qing-Qing Fu, Ji-Ying Zhou, Li-Xue Chen
BACKGROUND: Although the mechanism of chronic migraine (CM) is unclear, it might be related to central sensitization and neuronal persistent hyperexcitability. The tyrosine phosphorylation of NR2B (NR2B-pTyr) reportedly contributes to the development of central sensitization and persistent pain in the spinal cord. Central sensitization is thought to be associated with an increase in synaptic efficiency, but the mechanism through which NR2B-pTyr regulates synaptic participation in CM-related central sensitization is unknown...
November 6, 2018: Journal of Headache and Pain
Gabriella Donà, Alessandra Andrisani, Elena Tibaldi, Anna Maria Brunati, Chiara Sabbadin, Decio Armanini, Guido Ambrosini, Eugenio Ragazzi, Luciana Bordin
Astaxanthin (Asta), red pigment of the carotenoid family, is known for its anti-oxidant, anti-cancer, anti-diabetic, and anti-inflammatory properties. In this study, we evaluated the effects of Asta on isolated human sperm in the presence of human papillomavirus (HPV) 16 capsid protein, L1. Sperm, purified by gradient separation, were treated with HPV16-L1 in both a dose and time-dependent manner in the absence or presence of 30 min-Asta pre-incubation. Effects of HPV16-L1 alone after Asta pre-incubation were evaluated by rafts (CTB) and Lyn dislocation, Tyr-phosphorylation (Tyr-P) of the head, percentages of acrosome-reacted cells (ARC) and endogenous reactive oxygen species (ROS) generation...
November 2, 2018: Marine Drugs
Michael T Ringel, Thomas Brüser
Pyoverdines are fluorescent siderophores of pseudomonads that play important roles for growth under iron-limiting conditions. The production of pyoverdines by fluorescent pseudomonads permits their colonization of hosts ranging from humans to plants. Prominent examples include pathogenic or non-pathogenic species such as Pseudomonas aeruginosa , P. putida , P. syringae , or P. fluorescens . Many distinct pyoverdines have been identified, all of which have a dihydroxyquinoline fluorophore in common, derived from oxidative cyclizations of non-ribosomal peptides...
August 28, 2018: Microbial Cell
Maria Cerrato-Alvarez, Elena Bernalte, María Josefa Bernalte-García, Eduardo Pinilla-Gil
In this work it is explored a real applicability of miniaturised and portable biosensing technology for the estimation of total phenolic content in 15 different commercial beers by applying direct amperometry. Gold nanoparticles screen-printed electrodes were thoroughly modified with tyrosinase (Tyr-AuNPS-SPCEs), which was immobilised on the surface by crosslinking with glutaraldehyde. All chemical and instrumental variables involved in the electrochemical method were optimised to develop a reliable and powerful tool to estimate rapidly the content of phenolic compounds in complex beer samples...
February 1, 2019: Talanta
Ighovie F Onojafe, Lucyanne H Megan, Madeline G Melch, Joseph O Aderemi, Ramakrishna P Alur, Mones S Abu-Asab, Chi-Chao Chan, Isa M Bernardini, Jessica S Albert, Tiziana Cogliati, David R Adams, Brian P Brooks
Purpose: Oral nitisinone has been shown to increase fur and ocular pigmentation in a mouse model of oculocutaneous albinism (OCA) due to hypomorphic mutations in tyrosinase (TYR), OCA1B. This study determines if nitisinone can improve ocular and/or fur pigmentation in a mouse model of OCA type 3 (OCA3), caused by mutation of the tyrosinase-related protein 1 (Tyrp1) gene. Methods: Mice homozygous for a null allele in the Tyrp1 gene (C57BL/6J-Tyrp1 b-J/J) were treated with 8 mg/kg nitisinone or vehicle every other day by oral gavage...
October 1, 2018: Investigative Ophthalmology & Visual Science
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