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developmental toxicity zebrafish

Miaomiao Teng, Wentao Zhu, Dezhen Wang, Jin Yan, Suzhen Qi, Min Song, Chengju Wang
Flutolanil, an amide fungicide, had been detected frequently in aquatic environments; it is thus potentially a great risk to aquatic organisms and human health. Therefore, we investigated the developmental toxicity and the potential mechanism of thyroid endocrine disruption induced by flutolanil based on 1 H NMR metabolomics analysis using a zebrafish model. Hatching of zebrafish embryo exposed to flutolanil was inhibited at 72 hpf (hour post-fertilization) and survival and body length at 96 hpf. In addition, increased teratogenic effects on embryos were observed, including pericardial edema, spine deformation, and tail malformation...
July 31, 2018: Environmental Pollution
Xishan Li, Guanghui Ding, Yijun Xiong, Xinrui Ma, Youmei Fan, Deqi Xiong
This study focused on comparing the lethal and sublethal toxicity of water-accommodated fractions (WAF) and chemically enhanced WAF (CEWAF) of crude oil to zebrafish (Danio rerio) on early life stages (ELS). Results showed that the addition of GM-2 dispersant caused an increase in the levels of total petroleum hydrocarbons (TPHs) and total priority polycyclic aromatic hydrocarbons (ΣPAHs). Based on ΣPAHs, the LC20 estimates for WAF and CEWAF were 4.88 µg L-1 and 1.19 µg L-1 , respectively, indicating that CEWAF was approximately four times more toxic...
August 13, 2018: Bulletin of Environmental Contamination and Toxicology
Javier Conde-Vancells, Mercedes Vazquez-Chantada, Catherine W McCollum, Maria Bondesson, Sharanya Maanasi Kalasekar, Bogdan J Wlodarczyk, Jan-Åke Gustafsson, Robert M Cabrera, Richard H Finnell
In this study we combine assays using mouse embryonic stem cells (mESCs) and zebrafish embryos to evaluate the potential developmental toxicity of industrial and pharmaceutical chemicals. A set of eleven chemicals of known mammalian in vivo teratogenicity were tested in the assays and correlations to mammalian data investigated. Using mESCs, proliferation, differentiation, and cytotoxicity of the chemicals were measured. In zebrafish embryos, lethality and the lowest effect level concentrations for morphological malformations were determined...
August 10, 2018: Reproductive Toxicology
Frank Alifui-Segbaya, Jasper Bowman, Alan R White, Sony Varma, Graham J Lieschke, Roy George
The paucity of information on the biological risks of photopolymers in additive manufacturing or three-dimensional printing (3DP) is a major challenge for the uptake of the technology in the construction of medical devices in dentistry. In this paper, the biocompatibility of additively manufactured methacrylates are evaluated using the innovative zebrafish (Danio rerio) embryo model. Data obtained confirmed gradations of toxicity primarily influenced by ethanol treatment, exposure scenarios, and extraction vehicles...
August 9, 2018: Acta Biomaterialia
Thayres S Andrade, Rhaul de Oliveira, Muriel Lopes, Marcus Vinicius Von Zuben, Cesar Koppe Grisolia, Inês Domingues, Eloisa Dutra Caldas, Aline Pic-Taylor
Ayahuasca is a psychoactive concoction prepared from the plants Banisteriopsis caapi and Psychotria viridis which are used ancestrally by Amazonian Indian populations and more recently, by Christian religious groups in Brazil and other countries. The aims of the present study were to identify the effects of ayahuasca on zebrafish embryo development and neurobehavior. Toxicity and developmental endpoints for zebrafish embryos were assessed from 0 to 1000 mg/L over 96 h of exposure. The effects on locomotor activity of zebrafish larvae were assessed using a video tracking system (ZebraBox) from 0 to 20 mg/L and after 120 and 144 h of exposure...
August 4, 2018: Chemico-biological Interactions
Fangjie Cao, Hui Li, Feng Zhao, Peizhuo Wu, Le Qian, Lan Huang, Sen Pang, Christopher J Martyniuk, Lihong Qiu
The fungicide azoxystrobin induces reproductive toxicity in adult zebrafish. However, data are lacking regarding the impact of azoxystrobin in the F1 generation after parental exposure. To address this knowledge gap, parental zebrafish (F0) were exposed to 2, 20 and 200 μg/L azoxystrobin for 21 days. Following this, fertilized F1 embryos from the exposed parents were either exposed to the same concentration as their corresponding exposed parents (F0+/F1+) or were reared in clean water (F0+/F1-) for 96 h ("+", exposed; "-" unexposed)...
July 24, 2018: Science of the Total Environment
Luís M Félix, Ana Luzio, Maria Themudo, Luís Antunes, Manuela Matos, Ana M Coimbra, Ana M Valentim
MS-222 has been widely used as an anaesthetic in fish, thus, raising the need to infer about its toxicological safety during development. In this study, MS-222 toxicity in zebrafish embryos was evaluated after a 20-min exposure at different stages of development. Embryos exposed during the 256-cell stage displayed an increase in mortality, associated with defective early developmental pathways. Following exposure during the 50% epiboly stage, an increase in mortality and abnormal cartilage development, as well as changes in noggin expression were observed...
July 24, 2018: Reproductive Toxicology
Supratik Kar, Shinjita Ghosh, Jerzy Leszczynski
Halogenated chemicals including perfluoroalkyl substances (PFASs) represent an emerging class of endocrine-disrupting pollutants for human populations across the globe. Distress related to their environmental fate and toxicity has initiated several research projects, but the amount of experimental data available for these pollutants is limited. The objective of this study is to assess the toxicity of potentially "safer" alternatives, in relation to their existing counterparts. Developmental toxicity data on zebrafish (Danio rerio) embryos of single and tertiary halogenated mixtures were modeled employing quantitative structure-toxicity relationship (QSTR) tool...
July 12, 2018: Chemosphere
Ying Ying Chen, King Ming Chan
Trace metal ions such as cadmium (Cd2+ ) and trace organics typified by 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) are common co-contaminants in the environment and cause toxic effects in aquatic organisms that pose serious health risks. We studied the effects of Cd2+ on the regulation of cytochrome P450 1A1 (cyp1a1) gene-induction by TCDD using zebrafish embryos and larvae and adult zebrafish tissues. Our results showed that TCDD induced the cyp1a1 gene in all developmental stages and tissues of zebrafish, and the induction was higher in females than males...
July 12, 2018: Chemosphere
Marjorie Alvarez, Myra N Chávez, Miguel Miranda, Geraldine Aedo, Miguel L Allende, José T Egaña
Cigarette smoke is associated with several pathologies including chronic respiratory diseases and cancer. In addition, exposure to cigarette smoke is correlated with impaired wound healing, where a significant decrease in the regenerative capacity of smokers is well documented and broadly considered a negative risk factor after trauma or surgery. So far, some in vitro and in vivo models have been described to study how exposure to cigarette smoke diminishes the regenerative potential in different organisms...
July 19, 2018: Scientific Reports
Andrey Massarsky, Nishad Jayasundara, Lilah Glazer, Edward D Levin, G L Prasad, Richard T Di Giulio
The effects of prenatal exposure to cigarette smoke remain a subject of major interest, especially as it relates to neural development and adverse behavioral outcomes. Several studies have investigated the developmental toxicity of cigarette smoke components in a zebrafish model, showing that developmental exposure to total particulate matter (TPM; particulate phase of cigarette smoke) leads to adverse physiological aberrations and locomotor hyperactivity. Thus, the current study examines whether developmental TPM exposure of zebrafish embryos/larvae (F0) leads to physiological and behavioral alterations, and whether adverse effects are observed in adult fish and the next generation (F1; i...
July 17, 2018: Neurotoxicology
Katharine A Horzmann, Leeah S Reidenbach, Devang H Thanki, Anna E Winchester, Brad A Qualizza, Geoffrey A Ryan, Kaitlyn E Egan, Victoria E Hedrick, Tiago J P Sobreira, Samuel M Peterson, Gregory J Weber, Sara E Wirbisky-Hershberger, Maria S Sepúlveda, Jennifer L Freeman
Atrazine (ATZ), the second most commonly used herbicide in the United States, is an endocrine disrupting chemical linked to cancer and a common drinking water contaminant. This study further investigates ATZ-related developmental toxicity by testing the following hypotheses in zebrafish: the effects of embryonic ATZ exposure are dependent on timing of exposure; embryonic ATZ exposure alters brain development and function; and embryonic ATZ exposure changes protein abundance in carcinogenesis-related pathways...
August 30, 2018: Journal of Proteomics
Danielle Hagstrom, Lisa Truong, Siqi Zhang, Robert Tanguay, Eva-Maria S Collins
There is a clear need to establish and validate new methodologies to more quickly and efficiently screen chemicals for potential toxic effects, particularly on development. The emergence of alternative animal systems for rapid toxicology screens presents valuable opportunities to evaluate how systems complement each other. In this article, we compare a chemical library of 87-compounds in two such systems, developing zebrafish and freshwater planarians, by screening for developmental neurotoxic effects. We show that the systems' toxicological profiles are complementary to each other, with zebrafish yielding more detailed morphological endpoints and planarians more behavioral endpoints...
July 11, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Abdolrahman Khezri, Juan G Herranz-Jusdado, Erik Ropstad, Thomas Wk Fraser
Mycotoxins are secondary metabolites produced by varieties of fungi that contaminate food and feed resources and are capable of inducing a wide range of toxicity. In the current study, we investigated developmental and behavioural toxicity in zebrafish larvae after exposure to six different mycotoxins; ochratoxin A (OTA), type A trichothecenes mycotoxin (T-2 toxin), type B trichothecenes mycotoxin (deoxynivalenol - DON), and zearalenone (ZEN) and its metabolites alpha-zearalenol (α-ZOL) and beta-zearalenol (β-ZOL)...
July 3, 2018: Environmental Pollution
Xiaoxi Yang, Zhendong Sun, Wanyi Wang, Qunfang Zhou, Guoqing Shi, Fusheng Wei, Guibin Jiang
Synthetic phenolic antioxidants (SPAs) have gained high concerns due to their extensive usages and unintended environmental release via various routes. Their contamination in water system could pose potential threat to aquatic organisms, therefore, the studies on the aquatic toxicology of this kind of chemicals are of high importance. In this research, the developmental toxicities of four commonly used SPAs, including butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tert-butyl hydroquinone (TBHQ), and 2,2'-methylenebis (6-tert-butyl-4-methylphenol) (AO2246) were investigated using the zebrafish embryo toxicity test (ZFET)...
June 23, 2018: Science of the Total Environment
Vincent Laizé, Paulo J Gavaia, Marco Tarasco, Michael N Viegas, Joana Caria, Nuno Luis, M Leonor Cancela
Many chemicals produced by human activities end up in the aquatic ecosystem causing adverse developmental and reproductive effects in aquatic organisms. There is evidence that some anthropogenic chemicals disturb bone formation and skeletal development but the lack of suitable in vitro and in vivo systems for testing has hindered the identification of underlying mechanisms of osteotoxicity. Several fish systems - an in vitro cell system to study extracellular matrix mineralization and in vivo systems to evaluate bone formation and skeletogenesis - were combined to collect data on the osteotoxic activity of 3-methylcholanthrene (3-MC), a polycyclic aromatic hydrocarbon...
June 22, 2018: Ecotoxicology and Environmental Safety
Yongmei Fan, Weiguo Miao, Kehua Lai, Weikang Huang, RuiXue Song, Qing X Li
Hymexazol is an efficacious and widely used fungicide. However, its environmental toxicological assessment has not been well documented. It had no report of its toxicity to fish embryo. Fish embryo acute toxicity tests are highly predictive of aquatic embryotoxicity outcome. In this study, zebrafish (Danio rerio) embryos were exposed to hymexazol at varying concentrations for the study of the developmental toxicity, melanin biosynthesis, biochemical and transcriptional endpoints. The embryotoxicity tests indicated that the 96h LC50 value of hymexazol was 649mg/L with a 95% confidence interval range of 632-667mg/L...
May 2018: Pesticide Biochemistry and Physiology
Tianyu Yu, Yue Jiang, Sijie Lin
The zebrafish is a globally recognized fresh water organism frequently used in developmental biology, environmental toxicology, and human disease related research fields. Thanks to its unique features, including large fecundity, embryo translucency, rapid and simultaneous development, etc., zebrafish embryos are often used for large scale toxicity assessment of chemicals and drug/compound screening. A typical screening procedure involves adult zebrafish spawning, embryos selection, and arraying the embryos into multi-well plates...
June 1, 2018: Journal of Visualized Experiments: JoVE
Yi Liu, Muhammad Junaid, Yan Wang, Yu-Mei Tang, Wan-Ping Bian, Wen-Xu Xiong, Hai-Yang Huang, Chun-Di Chen, De-Sheng Pei
Tetracycline hydrochloride (TH), indomethacin (IM), and bezafibrate (BF) belong to the three different important classes of pharmaceuticals, which are well known for their toxicity and environmental concerns. However, studies are still elusive to highlight the mechanistic toxicity of these pharmaceuticals and rank them using both, the toxicity prediction and confirmation approaches. Therefore, we employed the next generation toxicity testing in 21st century (TOX21) tools and estimated the in vitro/vivo toxic endpoints of mentioned pharmaceuticals, and then confirmed them using in vitro/vivo assays...
August 2018: Aquatic Toxicology
Xiyan Mu, Ying Huang, Jia Li, Ke Yang, Wenbo Yang, Gongming Shen, Xuxing Li, Yunlei Lei, Sen Pang, Chengju Wang, Xuefeng Li, Yingren Li
To investigate the biological pathways involved in phthalate-induced developmental effects, zebrafish embryos were exposed to different concentrations of di-(2-ethylhexyl) (DEHP) and di-butyl phthalate (DBP) for 96 h. Embryonic exposure to DEHP and DBP induced body length decrease, yolk sac abnormities, and immune responses (up-regulation of immune proteins and genes). The lipidomic results showed that at a concentration of 50 μg/L, DEHP and DBP significantly reduced the levels of fatty acids, triglycerides, diacylglycerol, and cholesterol...
October 2018: Environmental Pollution
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