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developmental toxicity zebrafish

Guanghua Xiong, Lufang Zou, Yunyun Deng, Yunlong Meng, Xinjun Liao, Huiqiang Lu
Clethodim is one of the most widely used herbicides in agriculture, but its potential negative effects on aquatic organisms are still poorly understood. This study examined the effects of clethodim on zebrafish at aspects of early stage embryonic development, immune toxicity, cell apoptosis and locomotor behavior. Firstly, clethodim exposure markedly decreased the survival rate, body length, and heart rate and resulted in a series of morphological abnormalities, primarily spinal deformities (SD) and yolk sac edema, in zebrafish larvae...
December 2, 2018: Fish & Shellfish Immunology
Hong-Jie Sun, Yu Zhang, Jing-Ying Zhang, Hongjun Lin, Jianrong Chen, Huachang Hong
2,6-dichlorobenzoquinone (2,6-DCBQ), an emerging disinfection by-production, frequently occurs in reclaimed water and drinking water. However, limited information was available regarding its toxicity. To evaluate its impact, zebrafish at early life stage were exposed to 0, 10, 30, 60, 90, or 120 μg L-1 2,6-BDCQ for 72 h. Our results indicated that 2,6-BDCQ decreased zebrafish's survival rate to 65% and 44% at 90 and 120 μg L-1 treatments and increased its aberration rate to 11% and 26% at 90 μg L-1 and 120 μg L-1 treatments...
November 22, 2018: Environmental Pollution
Guijin Sun, Yingqiu Li
Dibutyl phthalate (DBP) is one of the most ubiquitous plasticizers used worldwide and has been frequently detected in soil, water, atmosphere, and other environmental media. DBP has become a ubiquitous environment contaminant and causes serious pollution. However, much attention has been paid to the toxicity of DBP, with only limited attention paid to its detrimental effects on the heart. In the present study, we investigated the toxicity of DBP in zebrafish embryo development, especially adverse effects on cardiac development...
November 15, 2018: Chemosphere
Juanjuan Li, Yun Zhang, Kechun Liu, Qiuxia He, Chen Sun, Jian Han, Liwen Han, Qingping Tian
The aim of the study was to determine the developmental toxicity of the traditional Chinese medicine Xiaoaiping (XAP) and to investigate its underlying mechanism of action. Zebrafish embryos were incubated with 0.4, 0.8, 1.2, and 1.6 mg/mL XAP. Endpoints such as mortality, hatching rate, malformation, body length, morphology score, swimming behavior, histological changes, reactive oxygen species (ROS) production, total superoxide dismutase (T-SOD) activity, and the mRNA expression of genes related to oxidative stress, endoplasmic reticulum (ER) stress, apoptosis, and the Wnt pathway were evaluated...
2018: Frontiers in Pharmacology
Karthiga P, Ponnanikajamideen M, R Samuel Rajendran, Gurusamy Annadurai, S Rajeshkumar
Zirconia oxide nanoparticles (ZrO2 NPs) are known to be one of the neutral bioceramic metal compounds that has been widely used for their beneficial applications in many biomedical areas, in dental implants, bone joint replacements, drug delivery vehicles, and in various industrial applications. To study the effects of ZrO2 NPs on zebrafish model, we used early life stages of the zebrafish (Danio rerio) to examine such effects on embryonic development in this species. ZrO2 NPs were synthesized by the sol-gel method, size about 15-20 nm and characterized by SEM, EDX, XRD, FTIR, UV-Vis Spectra...
November 20, 2018: Drug and Chemical Toxicology
Lianguo Chen, Chenyan Hu, Yongyong Guo, Qipeng Shi, Bingsheng Zhou
Titanium dioxide (TiO2 ) nanoparticles and bisphenol A (BPA) in aquatic environments interact reciprocally to enhance the maternal transfer of pollutants to offspring, thus varying the innate toxicities during early embryonic development. However, it remains unexplored regarding the molecular mechanisms of developmental toxicity in offspring after parental coexposure. In the present study, adult zebrafish were exposed to TiO2 nanoparticles (100 μg/L), BPA (20 μg/L) or their binary mixture for four months...
November 12, 2018: Chemosphere
Samantha Carrillo-Rosas, Chantal Weber, Lorraine Fievet, Nadia Messaddeq, Alice Karam, Yvon Trottier
Polyglutamine expansion in Ataxin-7 results in Spinocerebellar ataxia type 7 (SCA7) and causes visual impairment. SCA7 photoreceptors progressively lose their outer segments, a structure essential for their visual function. Ataxin-7 is a subunit of the transcriptional coactivator Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex, implicated in the development of the visual system in flies. To determine the function of Ataxin-7 in the vertebrate eye, we have inactivated Ataxin-7 in zebrafish. While Ataxin-7 depletion in flies led to gross retinal degeneration, in zebrafish it primarily results in ocular coloboma, a structural malformation responsible for pediatric visual impairment in human...
November 16, 2018: Human Molecular Genetics
Xuefang Liang, Ondrej Adamovsky, Christopher L Souders, Christopher J Martyniuk
Among the benzotriazole ultraviolet stabilizers (BUVSs), UV-234 and UV-320 are frequently detected in aquatic ecosystem. Despite the fact that these chemicals are present in low ng/L levels in surface water, they show high bio-accumulation potential and pose exposure risks to aquatic organisms. However, there are limited toxicological data available in fish. In this study, zebrafish embryos were exposed to 0.01, 0.1 and 1 μM UV-234 or UV-320 for up to 6 days. Developmental toxicity as well as effects on mitochondrial bioenergetics, immune system responses, and locomotor activity in zebrafish were measured...
November 2, 2018: Environmental Pollution
Omar Issa, Ezdihar A Hassoun, Frederick E Williams
To assess the developmental toxicity of trichloroacetate (TCA), zebrafish embryos were exposed to 8 to 48 mM of TCA and evaluated for developmental milestones from 8- to 144-hour postfertilization (hpf). All developmental toxicities are reported in this paper. Embryos were found to have developed edema in response to 16 to 48 mM of TCA exposure at 32- to 80-hpf, experienced delay in hatching success in response to 24 to 48 mM at 80-hpf. Lordosis was observed in developing embryos exposed to 40 to 48 mM at 55- to 144-hpf...
November 15, 2018: Journal of Biochemical and Molecular Toxicology
Vani Joshi, Katti Pancharatna
In this study, we tested the teratogenic/embryotoxic potentials of food colorant, Sunset Yellow (E110) using zebrafish embryos as a model. Laboratory-raised developing embryos of Danio rerio were exposed to graded concentrations (00, 0.1, 1.0, 2.0, 3.0, 4.0, 5.0, 10, 20, 30, 40, 50 and 100 mm) of E110 from gastrulation stage (~6 hours post-fertilization [hpf]) up until hatching. The developmental trajectory of each embryo and post-hatched larva was traced from 24 to 168 hpf. The no observed effect concentration (NOEC), median effective concentration (EC50 ), median lethal concentration (LC50 ) and teratogenic index were determined...
November 13, 2018: Journal of Applied Toxicology: JAT
Elodie Falisse, Bertrand Ducos, Peter A Stockwell, Ian M Morison, Aniruddha Chatterjee, Frédéric Silvestre
There is increasing evidence that toxicant exposure can alter DNA methylation profile, one of the main epigenetic mechanisms, particularly during embryogenesis when DNA methylation patterns are being established. In order to investigate the effects of the antibacterial agent Triclosan on DNA methylation and its correlation with gene expression, zebrafish embryos were exposed during 7 days post-fertilization (starting at maximum 8-cells stage) to 50 and 100 μg/l, two conditions for which increased sensitivity and acclimation have been respectively reported...
December 2018: Environmental Pollution
Života Selaković, Julie P Tran, Krishna P Kota, Marija Lazić, Cary Retterer, Robert Besh, Rekha G Panchal, Veronica Soloveva, Vantongreen A Sean, Wells B Jay, Aleksandar Pavić, Tatjana Verbić, Branka Vasiljević, Kathleen Kuehl, Allen J Duplantier, Sina Bavari, Rajini Mudhasani, Bogdan A Šolaja
Ebola virus (EBOV) causes a deadly hemorrhagic fever in humans and non-human primates. There is currently no FDA-approved vaccine or medication to counter this disease. Here, we report on the design, synthesis and anti-viral activities of two classes of compounds which show high potency against EBOV in both in vitro cell culture assays and in vivo mouse models Ebola viral disease. These compounds incorporate the structural features of cationic amphiphilic drugs (CAD), i.e they possess both a hydrophobic domain and a hydrophilic domain consisting of an ionizable amine functional group...
October 31, 2018: European Journal of Medicinal Chemistry
Xing Chen, Mingliang Fang, Melissa Chernick, Feng Wang, Jingfeng Yang, Yongli Yu, Na Zheng, Hiroki Teraoka, Satomi Nanba, Takeo Hiraga, David E Hinton, Wu Dong
Thiram, a pesticide in the dithiocarbamate chemical family, is widely used to prevent fungal disease in seeds and crops. Its off-site movement to surface waters occurs and may place aquatic organisms at potential harm. Zebrafish embryos were used for investigation of acute (1 h) thiram exposure (0.001-10 µM) at various developmental stages. Survival decreased at 1 µM and 10 µM and hatching was delayed at 0.1 µM and 1 µM. Notochord curvatures were seen at 0.1 and 1 μM thiram when exposure was initiated at 2 and at 10 hpf...
November 5, 2018: General and Comparative Endocrinology
David E Hogan, Fei Tian, Scott W Malm, Christopher Olivares, Ricardo Palos Pacheco, Michael T Simonich, Anoop S Hunjan, Robert L Tanguay, Walter T Klimecki, Robin Polt, Jeanne E Pemberton, Joan E Curry, Raina M Maier
Synthetic monorhamnolipids differ from biologically produced material because they are produced as single congeners, depending on the β-hydroxyalkanoic acid used during synthesis. Each congener is produced as one of four possible diastereomers resulting from two chiral centers at the carbinols of the lipid tails [(R,R), (R,S), (S,R) and (S,S)]. We compare the biodegradability (CO2 respirometry), acute toxicity (Microtox assay), embryo toxicity (Zebrafish assay), and cytotoxicity (xCELLigence and MTS assays) of synthetic rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-C10-C10) monorhamnolipids against biosynthesized monorhamnolipid mixtures (bio-mRL)...
October 31, 2018: Journal of Hazardous Materials
Tao Chen, Hongmei Jin, Huimin Wang, Yugang Yao, Stanley Aniagu, Jian Tong, Yan Jiang
Ambient fine particulate matter (PM2.5 ) has been found to be associated with congenital heart defects, but the molecular mechanisms remain to be elucidated. Our previous study revealed that extractable organic matter (EOM) from PM2.5 exerted cardiac developmental toxicity in zebrafish embryos. The aim of the current study is to explore the effects of EOM on cardiac differentiation of P19 mouse embryonic carcinoma stem cells. We found that EOM at 10 μg/ml (a non-cytotoxic dose level) significantly reduced the proportion of cardiac muscle troponin (cTnT) positive cells and the percentage of spontaneously beating embryoid bodies, indicating a severe inhibition of cardiac differentiation...
February 2019: Chemosphere
Ava Faridi, Yunxiang Sun, Yutaka Okazaki, Guotao Peng, Jie Gao, Aleksandr Kakinen, Pouya Faridi, Mei Zhao, Ibrahim Javed, Anthony W Purcell, Thomas P Davis, Sijie Lin, Reiko Oda, Feng Ding, Pu Chun Ke
Amyloid fibrils generally display chirality, a feature which has rarely been exploited in the development of therapeutics against amyloid diseases. This study reports, for the first time, the use of mesoscopic chiral silica nanoribbons against the in vivo amyloidogenesis of human islet amyloid polypeptide (IAPP), the peptide whose aggregation is implicated in type 2 diabetes. The thioflavin T assay and transmission electron microscopy show accelerated IAPP fibrillization through elimination of the nucleation phase and shortening of the elongation phase by the nanostructures...
November 2018: Small
Katharina Dach, Bianca Yaghoobi, Martin R Schmuck, Dennis R Carty, Kelly M Morales, Pamela J Lein
To screen the tens of thousands of chemicals for which no toxicity data currently exists, it is necessary to move from in vivo rodent models to alternative models, such as zebrafish. Here, we used dechorionated Tropical 5D wildtype zebrafish embryos to screen a 91-compound library provided by the National Toxicology Program (NTP) for developmental toxicity. This library contained 86 unique chemicals that included negative controls, flame retardants, polycyclic aromatic hydrocarbons (PAHs), drugs, industrial chemicals and pesticides...
October 26, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Fan Wang, Ruiling Wang, Fei Liu, Wanguang Chen
Triclosan (TCS) is a broad-spectrum antibacterial agent and has been widely used in a diversity of personal care products. However, recent studies suggest that TCS has some adverse effects, and some evidences suggest in vitro neurotoxicity and developmental neurotoxicity of embryos. There are currently few studies concerning the mechanisms of TCS induced late developmental neurotoxic effect. Therefore, we investigated effects of juvenile zebrafish (Danio rerio) exposure to TCS. Male juvenile zebrafish were exposed to 68...
October 16, 2018: Toxicology and Applied Pharmacology
Jing Si, Rong Zhou, Baoquan Zhao, Yi Xie, Lu Gan, Jinhua Zhang, Yupei Wang, Xin Zhou, Xiaotang Ren, Hong Zhang
The health-related effects of ionizing radiation on embryonic development and their underlying mechanisms are still unclear. The aim of this study was to investigate the role of Wnt signaling in mediating the developmental toxicity induced by heavy ion and proton radiation using zebrafish embryos. Zebrafish embryos were radiated with carbon ions or protons. HLY78, an activator of the Wnt signaling pathway, was added immediately after radiation. Carbon ion radiation induced a significant increase of mortality, and activating Wnt signaling using HLY78 after radiation significantly alleviated this stress...
October 12, 2018: Toxicology
Alexandra Ulin, Jake Henderson, Minh-Tam Pham, James Meyo, Yuying Chen, Sibel I Karchner, Jared V Goldstone, Mark E Hahn, Larissa M Williams
Interactions between regulatory pathways allow organisms to adapt to their environment and respond to stress. One interaction that has been recently identified occurs between the aryl hydrocarbon receptor (AHR) and the nuclear factor erythroid-2 related factor (NRF) family. Each transcription factor regulates numerous downstream genes involved in the cellular response to toxicants and oxidative stress; they are also implicated in normal developmental pathways. The zebrafish model was used to explore the role of AHR regulation of nrf genes during development and in response to toxicant exposure...
October 13, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
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