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developmental toxicity zebrafish

Jing Si, Rong Zhou, Baoquan Zhao, Yi Xie, Lu Gan, Jinhua Zhang, Yupei Wang, Xin Zhou, Xiaotang Ren, Hong Zhang
The health-related effects of ionizing radiation on embryonic development and their underlying mechanisms are still unclear. The aim of this study was to investigate the role of Wnt signaling in mediating the developmental toxicity induced by heavy ion and proton radiation using zebrafish embryos. Zebrafish embryos were radiated with carbon ions or protons. HLY78, an activator of the Wnt signaling pathway, was added immediately after radiation. Carbon ion radiation induced a significant increase of mortality, and activating Wnt signaling using HLY78 after radiation significantly alleviated this stress...
October 12, 2018: Toxicology
Alexandra Ulin, Jake Henderson, Minh-Tam Pham, James Meyo, Yuying Chen, Sibel I Karchner, Jared V Goldstone, Mark E Hahn, Larissa M Williams
Interactions between regulatory pathways allow organisms to adapt to their environment and respond to stress. One interaction that has been recently identified occurs between the aryl hydrocarbon receptor (AHR) and the nuclear factor erythroid-2 related factor (NRF) family. Each transcription factor regulates numerous downstream genes involved in the cellular response to toxicants and oxidative stress; they are also implicated in normal developmental pathways. The zebrafish model was used to explore the role of AHR regulation of nrf genes during development and in response to toxicant exposure...
October 13, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Jui-Hua Hsieh, Kristen Ryan, Alexander Sedykh, Ja-An Lin, Andrew J Shapiro, Frederick Parham, Mamta Behl
Over the past decade, the zebrafish is increasingly being used as a model to screen for chemical-mediated toxicities including developmental toxicity (DT) and neurotoxicity (NT). One of the major challenges is lack of harmonization in data analysis approaches, thereby posing difficulty in comparing findings across laboratories. To address this, we sought to establish a unified data analysis strategy for both DT and NT data, by adopting the benchmark concentration (BMC) analysis. There are two critical aspects in the BMC analysis: having a toxicity endpoint amenable for BMC and selecting a proper benchmark response (BMR) for the endpoint...
October 13, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Tara R Catron, Scott P Keely, Nichole E Brinkman, Todd J Zurlinden, Charles E Wood, Justin R Wright, Drake Phelps, Emily Anneken Wheaton, Allison Kvasnicka, Shaza Gaballah, Regina Lamendella, Tamara Tal
Host-associated microbiota can biotransform xenobiotics, mediate health effects of chemical exposure, and play important roles in early development. Bisphenol A (BPA) is a widespread environmental chemical that has been associated with adverse endocrine and neurodevelopmental effects, some of which may be mediated by microbiota. Growing public concern over the safety of BPA has resulted in its replacement with structurally similar alternatives. In this study, we evaluated whether BPA and BPA alternatives alter microbiota and modulate secondary adverse behavioral effects in zebrafish...
October 13, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Ainhoa Alzualde, Mamta Behl, Nisha S Sipes, Jui-Hua Hsieh, Aintzane Alday, Raymond R Tice, Richard S Paules, Arantza Muriana, Celia Quevedo
Following the voluntary phase-out of brominated flame retardants (BFRs) due to their environmental persistence and toxicity, the organophosphorus flame retardants (OPFRs) are emerging replacements. However, there is limited information on the potential human health effects of the OPFRs. Zebrafish embryos are a viable vertebrate model organism with many advantages for high throughput testing toward human hazard assessment. We utilized zebrafish embryos to assess developmental toxicity, neurotoxicity, cardiotoxicity and hepatotoxicity, of eight replacement OPFRs: (triphenyl phosphate [TPHP], isopropylated phenyl phosphate [IPP], 2-ethylhexyl diphenyl phosphate [EHDP], tert-butylated phenyl diphenyl phosphate [BPDP], trimethyl phenyl phosphate [TMPP], isodecyl diphenyl phosphate [IDDP], tris(1,3-dichloroisopropyl) phosphate [TDCIPP], and tris(2-chloroethyl) phosphate [TCEP]) and two BFRs (3,3',5,5'- tetrabromobisphenol A [TBBPA] and 2,2'4,4'-brominated diphenyl ether [BDE-47])...
October 9, 2018: Neurotoxicology and Teratology
Zhaoyang Yan, Xiaoyong Huang, Yangzhouyun Xie, Meirong Song, Kui Zhu, Shuangyang Ding
Macrolide antibiotics (MALs) are widely used for both human and animal health. Most MALs and their metabolites transfer into aquatic organisms and environment resulting in violent consequences. Previous studies show that MALs cause cardiotoxicity in humans and mammals. However, the potential risk of these chemicals in aquatic organisms remains unclear. Here, we used zebrafish embryos as a model to evaluate the toxicity of MALs. Zebrafish embryos were exposed to four typical MALs including azithromycin (AZM), clarithromycin (CLR), tilmicosin (TMS) and tylosin (TYL) to study their cardiotoxicity...
February 1, 2019: Science of the Total Environment
Zhongjie Bai, Jinlong Zhang, Qiuping Zhang, Taofeng Zhang, Jili Li, Quanyi Zhao, Zhen Wang, Dian He, Jie Cheng, Jingke Zhang, Bin Liu
A series of H2 SCO dual-donors [Mn(CO)4 CS2 NR1 R2 ] was synthesized, and evaluated from toxicity and bioactivity. The COH2 S measuring test showed all the complexes not only released CO, but released H2 S. The resulting data of cytotoxicity showed all the complexes had activities against the cell proliferation; among them, complexes 1, 2 and 7 displayed higher activities than the others, and their potencies were close to cis-platinum (DDP); whereas the precursors A1 -A22 had almost no activities against all five tumor cell lines and W138 cell line proliferation...
November 5, 2018: European Journal of Medicinal Chemistry
Kyeongnam Kim, Chi-Hwa Wang, Yong Sik Ok, Sung-Eun Lee
This study assessed the developmental toxicities of water-soluble carbon black wastes (CBW) extract (1:5, w/v) in zebrafish embryos (Danio rerio). Acute embryonic toxicity was performed following OECD guideline 236. Analysis using ICP-OES revealed that nickel (Ni) and vanadium (V) were predominant in CBW. Embryos exposed to CBW exhibited developmental delay, along with pericardial and yolk sac edemas. Malformed heart chambers were found in the CBW-exposed embryos and heart rates were significantly reduced since 48 h post fertilization (hpf)...
October 1, 2018: Journal of Hazardous Materials
Elisabet Teixidó, Tobias R Kießling, Eckart Krupp, Celia Quevedo, Arantza Muriana, Stefan Scholz
Detection of developmental phenotypes in zebrafish embryos typically involves a visual assessment and scoring of morphological features by an individual researcher. Subjective scoring could impact results and be of particular concern when phenotypic effect patterns are also used as a diagnostic tool to classify compounds. Here we introduce a quantitative morphometric approach based on image analysis of zebrafish embryos. A software called FishInspector was developed to detect morphological features from images collected using an automated system to position zebrafish embryos...
October 8, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Fan Wang, Fei Liu, Wanguang Chen
Triclosan (TCS) is a broad-spectrum antibacterial agent which is widely used in various personal care products and cosmetics. It has been found that TCS affects endocrine, immune, nervous, reproductive, and developmental system. Although microRNAs (miRNAs) act a pivotal part in lots of metabolic activities, whether and how they are related to the process of TCS-induced toxicity is unknown. In the present study, TCS induced changes in miRNAs and target gene expression in male zebrafish (Danio rerio) brain, and the potential mechanism was studied...
September 29, 2018: Chemosphere
Longsheng Chen, Minjie Xu, Zhunan Gong, Samkele Zonyane, Shuwen Xu, Nokwanda P Makunga
BACKGROUND: Sutherlandia frutescens is one of the most promising commercialized, indigenous and medicinal plants of South Africa that is used as an immune-booster, and a traditional treatment for cancer. However, few studies report on its toxicology and dosage in vivo. There is still room to better understand its cytotoxicity effects in animal systems. METHODS: We prepared two extracts, one with 80% (v/v) ethanol, and the other, with water. Both were studied to determine the maximum tolerable concentration when extracts were applied at 0 to 200 μg/ml to a Tuebingen zebrafish embryo line...
October 5, 2018: BMC Complementary and Alternative Medicine
Fangjie Cao, Christopher L Souders, Pengfei Li, Sen Pang, Lihong Qiu, Christopher J Martyniuk
The objectives of this study were to compare the biological responses in developing zebrafish to two organophosphate insecticides, chlorpyrifos (CPF) and diazinon (DZN). Zebrafish embryos were exposed to either solvent control (0.1% DMSO, v/v), or one dose of 0.1, 1.0, 10.0 and 25.0 μM CPF, as well as one dose of 0.1, 1.0, 10.0 and 100.0 μM DZN for 96 h. CPF at 10.0 and 25.0 μM caused 70-80% and 100% mortality in embryos after 96 h exposure, whereas embryos treated with 10.0 and 100.0 μM DZN showed 30-40% and 70-80% lethality...
October 2, 2018: Neurotoxicology and Teratology
Fangjie Cao, Christopher L Souders, Pengfei Li, Ondrej Adamovsky, Sen Pang, Lihong Qiu, Christopher J Martyniuk
Ziram is a broad spectrum pesticide that belongs to the class of dimethyl-dithiocarbamate (DTC) fungicides. The objectives of this study were to assess the effects of ziram in developing zebrafish. Ziram was highly toxic to zebrafish embryos, with a 96-h LC50 value of 1082.54 nM (∼0.33 mg/L). Zebrafish embryos at 6 h post-fertilization (hpf) were exposed to solvent control (0.1% DMSO), or one dose of 1, 10, 100, and 1000 nM ziram for 96 h. Ziram induced lethality in a dose-dependent manner, decreased hatching rate and heartbeat, and caused wavy deformities at 72 and 96 hpf at 100 and 1000 nM...
September 19, 2018: Chemosphere
Heidi Wirt, Roselea Botka, Kathryn E Perez, Tisha King-Heiden
The ubiquitous and persistent contaminant, triclosan (TCS), is known to cause developmental and behavioral toxicity in fish, but few studies have evaluated the long-term effects of these responses. Here we use a phenotypically anchored approach to evaluate the behavioral responses caused by early exposure to environmentally relevant concentrations of triclosan (TCS) to better understand the risk TCS poses to fish. Zebrafish were exposed to 0, 0.4, 4, or 40 μg TCS/L (nominal concentrations) for 5 days followed by depuration for 16 days to assess effects on mortality, development, and foraging efficiency...
September 28, 2018: Environmental Toxicology and Chemistry
Basuvannan Rangasamy, Devan Hemalatha, Chellappan Shobana, Bojan Nataraj, Mathan Ramesh
Ketoprofen a nonsteroidal anti-inflammatory drug (NSAID) is widely used in over-the-counter to treat pain, swelling and inflammation. Due to extensive application these drugs has been detected in surface waters which may create a risk to aquatic organisms. The aim of the present study is to assess the ecotoxicity of ketoprofen at different concentrations (1, 10 and 100 μg/ml) on embryos and adult zebrafish (1, 10 and 100 μg L-1 ) under laboratory conditions. In embryos, concentration dependent developmental changes such as edema, spinal curvature, slow heartbeat, delayed hatching, and mortality rate were observed...
December 2018: Chemosphere
Eduardo A González, Dennis R Carty, Franklin D Tran, Austin M Cole, Pamela J Lein
Silver nanoparticles (Ag-NP) are ubiquitous in household and medical products because of their antimicrobial activity. A consequence of the high volume of Ag-NP production and usage is increased amounts of Ag-NPs released into the environment. Their small size (between 1-100 nanometers) results in unique physiochemical properties that may increase toxicity relative to their bulk counterpart. Therefore, the goal of this study is to assess the potential toxicity of environmentally relevant concentrations of Ag-NPs in zebrafish (Danio rerio)...
September 22, 2018: Environmental Toxicology and Chemistry
Michaela Liegertová, Dominika Wrobel, Regina Herma, Monika Müllerová, Lucie Červenková Šťastná, Petra Cuřínová, Tomáš Strašák, Marek Malý, Jan Čermák, Jiří Smejkal, Marcel Štofik, Jan Maly
Glycodendrimers (Glyco-DDMs) represent a rapidly growing class of nanoparticles with promising properties for biomedical applications but concerns regarding the impact on human health and environment are still justified. Here we report, for the first time, the comparative study of in vivo developmental toxicity of carbosilane Glyco-DDMs and their cytotoxicity in vitro. Carbosilane Glyco-DDMs (generation 1-3) containing 4, 8, and 16 β-d-glucopyranosyl units at the periphery (DDM1 Glu, DDM2 Glu, and DDM3 Glu) were synthesized and characterized by 1 H, 13 C and 29 Si NMR, mass spectrometry, dynamic light scattering, atomic force microscopy (AFM), and computer modeling...
September 5, 2018: Nanotoxicology
Bangeppagari Manjunatha, Sung Ho Park, Kiwoong Kim, Rajesh R Kundapur, Sang Joon Lee
The multiple effect of pristine graphene (pG) toxicity on cardiovascular developmental defects was assessed using zebrafish as a model. Recently, the nanotoxicity is emerging as a critical issue, and it is more significant in embryogenesis. Especially, graphene and its derivatives have attracted a lot of interest in biomedical applications. However, very little is known about the toxic effects of pG which has been widely used carbon nanomaterial according to concentration and its effects on biological and cardiovascular development...
December 2018: Environmental Pollution
S Karthick Raja Namasivayam, R S Arvind Bharani, Kiruthiga Karunamoorthy
In the present study, enhanced pesticidal activity and biocompatibility of chitosan nanocomposite prepared with biocompatible polymer chitosan - insecticidal metabolites derived from potential fungal biopesticidal agent Nomuraea rileyi were studied. Insecticidal metabolites were isolated from the culture filtrate of fungal strain grown in sabouraud maltose yeast extract broth (SMYB) and the collected filtrate was extracted with ethyl acetate followed by purification using G-60 silica gel column. Chitosan nanocomposite was prepared with metabolites thus acquired by ionic gelation method...
December 2018: International Journal of Biological Macromolecules
Subham Dasgupta, Vanessa Cheng, Sara M F Vliet, Constance A Mitchell, David C Volz
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is an organophosphate flame retardant used around the world. Within zebrafish, we previously showed that initiation of TDCIPP exposure during cleavage (0.75 h post-fertilization, hpf) results in epiboly disruption at 6 hpf, leading to dorsalized embryos by 24 hpf, a phenotype that mimics the effects of dorsomorphin (DMP), a bone morphogenetic protein (BMP) antagonist that dorsalizes embryos in the absence of epiboly defects. The objective of this study was to (1) investigate the role of BMP signaling in TDCIPP-induced toxicity during early embryogenesis, (2) identify other pathways and processes targeted by TDCIPP, and (3) characterize the downstream impacts of early developmental defects...
September 18, 2018: Environmental Science & Technology
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