Elena Fontana, Matilde Bongianni, Alberto Benussi, Erika Bronzato, Carlo Scialo, Luca Sacchetto, Annachiara Cagnin, Santina Castriciano, Emanuele Buratti, Fabrizio Gardoni, Maria Italia, Alberto Schreiber, Chiara Ferracin, Michele Fiorini, Kathy L Newell, Laura Cracco, Holly J Garringer, Maria Paola Cecchini, Magdalini Polymenidou, Alessandro Padovani, Salvatore Monaco, Giuseppe Legname, Bernardino Ghetti, Barbara Borroni, Gianluigi Zanusso
INTRODUCTION: We assessed TAR DNA-binding protein 43 (TDP-43) seeding activity and aggregates detection in olfactory mucosa of patients with frontotemporal lobar degeneration with TDP-43-immunoreactive pathology (FTLD-TDP) by TDP-43 seeding amplification assay (TDP43-SAA) and immunocytochemical analysis. METHODS: The TDP43-SAA was optimized using frontal cortex samples from 16 post mortem cases with FTLD-TDP, FTLD with tau inclusions, and controls. Subsequently, olfactory mucosa samples were collected from 17 patients with FTLD-TDP, 15 healthy controls, and three patients carrying MAPT variants...
October 31, 2023: Alzheimer's & Dementia: the Journal of the Alzheimer's Association