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Saartje Bloemen, Suzanne Zwaveling, Jonathan Douxfils, Mark Roest, Romy Kremers, François Mullier
INTRODUCTION: Calibrated automated thrombinography (CAT) is a sensitive method to assess coagulation. Dabigatran inhibits both free thrombin and the α2 macroglobulin (α2 M)-thrombin complex, which results in an erroneously increased peak and endogenous thrombin potential (ETP) without affecting lag time and time-to-peak. The aim of this study was to elucidate the artefacts in CAT when dabigatran is present. MATERIALS AND METHODS: Thrombin generation (TG) was measured in vitro by using CAT in the presence or absence of 6 μM idarucizumab in plasma spiked with dabigatran...
October 6, 2018: Thrombosis Research
Juliana Aparecida Cerqueira, Wilmer Alejandro Zamora Restan, Mayara Gonçalves Fonseca, Lucas Azevedo Catananti, Maria Luiza Mendes de Almeida, Walter Heinz Feringer Junior, Gener Tadeu Pereira, Aulus Cavalieri Carciofi, Guilherme de Camargo Ferraz
Few data exist on the physiological consequences of an incremental exercise test (IET) and training in dogs. Here we evaluated the effect of an endurance-training program (ETP) on the kinetics of the serum biomarkers: creatine kinase (CK), aspartate aminotransferase (AST), myoglobin, cardiac troponin I (cTnI), and creatine kinase isoenzyme MB (CK-MB). Eighteen Beagle dogs were assigned to three groups: sedentary (S), untrained (U), and trained (T). The dogs from the groups T and U underwent IET, and their lactate threshold (LT) was determined...
October 3, 2018: Research in Veterinary Science
Ping Wang, Xian'gui Peng, Xiaojuan Deng, Li Gao, Xi Zhang, Yimei Feng
RATIONALE: The diagnosis of hematological malignancies depends on laboratory analysis and often requires multiple experimental methods to judge, otherwise misdiagnosis is apt to happen. Lymph node biopsy immunohistochemistry (IHC) for T-lymphoblastic lymphoma (T-LBL) requires the establishment of antibody set screening. For identifying T-LBL and early T-cell precursor acute lymphoblastic leukemia (ETP-ALL) by lymph node biopsy and IHC, WHO has not yet proposed a better IHC antibody combination...
October 2018: Medicine (Baltimore)
M A Khosa, Aman Ullah
In this review, we discussed the micro-level aspects of protein supported biosorption. The mechanism, surface chemistry in terms of energy interactions and electron transfer process (ETP) of peptide systems within protein are three important areas that provide mechanistic insight into protein supported biosorption. The functional groups in proteinous material like hydroxyl (-OH), carbonyl (>C=O), carboxyl (-COOH) and sulfhydryl (-SH) play a significant role in the biosorption of variety of pollutants such as metal ions, metalloids, and organic matters in wastewaters...
September 30, 2018: Advances in Colloid and Interface Science
Brandon E Haines, Brandon M Nelson, Jessica M Grandner, Justin Kim, K N Houk, Mohammad Movassaghi, Djamaladdin G Musaev
The mechanism of permanganate-mediated dual C-H oxidation of complex diketopiperazines has been examined with density functional theory computations. The products of these oxidations are enabling intermediates in the synthesis of structurally diverse ETP natural products. We evaluated, for the first time, the impact of ion-pairing and aggregation states of the permanganate ion and counter-cations, such as bis(pyridine)-silver(I) (Ag+ ) and tetra- n-butylammonium (TBA+ ), on the C-H oxidation mechanism. The C-H abstraction occurs through an open shell singlet species, as noted previously, followed by O-rebound and a competing OH-rebound pathway...
October 17, 2018: Journal of the American Chemical Society
Subhasis Barik, Alexis N Cattin-Roy, Mindy M Miller, Tobechukwu K Ukah, Habib Zaghouani
Recently we reported that IL-4 and IL-13 signaling in murine early thymic progenitors (ETPs) expressing the heteroreceptor (HR) comprising IL-4 receptor α (IL-4Rα) and IL-13 receptor α 1 (IL-13Rα1) activate STAT6 and inhibit ETP maturation potential toward T cells. In this study, we asked whether IL-4 and IL-13 signaling through the HR mobilizes other STAT molecules to shape ETP fate decision. The findings indicate that HR+ ETPs undergoing cytokine signaling display increased STAT1, but not STAT3, phosphorylation in addition to STAT6 activation...
October 5, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
M Palova, L Slavik, A Hlusi, T Szotkowski, J Ulehlova, M Divoka, J Navratilova, T Papajik
BACKGROUND: Primary myelofibrosis (PMF) is a chronic clonal myeloid disorder. Together with essential thrombocythemia (ET) and polycythemia vera (PV), it belongs to a group of Philadelphia chromosome-negative myeloproliferative neoplasms. Thrombotic events are serious complications negatively influencing the quality and length of these patients' lives. The confirmed risk factors for venous thromboembolism are age over 60 years, a positive history of thromboembolism, presence of common cardiovascular risks, JAK2 V617F mutation and, according to some authors, leukocytosis...
September 1, 2018: Clinical Laboratory
Elias Kyriakou, Konstantinos Katogiannis, Ignatios Ikonomidis, George Giallouros, Georgios K Nikolopoulos, Evdoxia Rapti, Maria Taichert, Katerina Pantavou, Argiri Gialeraki, Foteini Kousathana, Aristarchos Poulis, Andreas G Tsantes, Stefanos Bonovas, Violetta Kapsimali, Georgios Tsivgoulis, Argirios E Tsantes
Our aim is to determine the most appropriate laboratory tests, besides anti-factor Xa (anti-FXa) chromogenic assays, to estimate the degree of anticoagulation with apixaban and compare it with that of rivaroxaban in real-world patients. Twenty patients with nonvalvular atrial fibrillation treated with apixaban 5 mg twice daily and 20 patients on rivaroxaban 20 mg once daily were studied. Conventional coagulation tests, thrombin generation assay (TGA), and thromboelastometry (nonactivated TEM [NATEM] assay) were performed in the 40 patients and 20 controls...
October 1, 2018: Clinical and Applied Thrombosis/hemostasis
Hui Zhang, Haishen Kong, Yunsong Yu, Anhua Wu, Qiong Duan, Xiaofeng Jiang, Shufang Zhang, Ziyong Sun, Yuxing Ni, Weiping Wang, Yong Wang, Kang Liao, Huayin Li, Chunxia Yang, Wenxiang Huang, Bingdong Gui, Bin Shan, Robert Badal, Qiwen Yang, Yingchun Xu
BACKGROUND: To evaluate the susceptibility rates of aerobic and facultative Gram-negative bacterial isolates from Chinese intra-abdominal infections (IAI) and urinary tract infections (UTI) focusing on carbapenems and comparing their effectiveness between 2014 and 2015. METHODS: A total of 2318 strains in 2015 (1483 from IAI and 835 from UTI) and 2374 strains in 2014 (1438 from IAI and 936 from UTI) were included in the analysis. Antimicrobial susceptibilities were determined at a central laboratory using CLSI broth microdilution and interpretive standards...
September 29, 2018: BMC Infectious Diseases
Nidhi Rohatgi, Wei Zou, Patrick L Collins, Jonathan R Brestoff, Timothy H Chen, Yousef Abu-Amer, Steven L Teitelbaum
Additional sex comb-like 1 ( ASXL1 ) mutations are commonly associated with myeloid malignancies and are markers of aggressive disease. The fact that ASXL1 is necessary for myeloid differentiation raises the possibility it also regulates osteoclasts. We find deletion of ASXL1 in myeloid cells results in bone loss with increased abundance of osteoclasts. Because ASXL1 is an enhancer of trithorax and polycomb (ETP) protein, we asked if it modulates osteoclast differentiation by maintaining balance between positive and negative epigenetic regulators...
October 9, 2018: Blood Advances
Mayada M Elgohary, Maged W Helmy, Sana M Mortada, Ahmed O Elzoghby
AIM: A Nano-in-Nano approach was exploited to facilitate incorporation of the chemotherapeutic drug etoposide (ETP) as nanosuspension, synergistically with berberine (BER) into hydrophilic albumin nanoparticles (HSA NPs). METHODS: For maximal tumor targeting, HSA was modified with mannose and phenyl-boronic acid. Furthermore, different crosslinkers were investigated for sustained release of water soluble BER from HSA NPs. RESULTS: The elaborated dual-targeted HSA NPs (216...
September 28, 2018: Nanomedicine
Jinjun Cheng, Matthew M Klairmont, John K Choi
BACKGROUND: Recent data have demonstrated the high sensitivity and specificity of peripheral blood flow cytometry (PBFC) for the diagnosis of pediatric leukemia; however, diagnostically significant immunophenotypic discrepancies between PBFC and bone marrow (BM) evaluation, which result in different lineage assignment and treatment protocols, can rarely occur. Here, we sought to further characterize the performance of PBFC for pediatric leukemia and highlight the exceptions when PBFC can result in misdiagnosis...
September 25, 2018: Pediatric Blood & Cancer
Changhu Lee, Min Kim, Jun Ho Lee, Jiyoung Oh, Hyun-Hee Shin, Sang Min Lee, Philipp E Scherer, Hyug Moo Kwon, Jang Hyun Choi, Jiyoung Park
Extracellular matrix dysregulation is associated with chronic liver disease. CollagenVI-alpha3 chain (COL6A3) is a biomarker for hepatic fibrosis and poor prognosis of hepatocellular carcinoma (HCC), but its function in liver pathology remains unknown. High levels of COL6A3 and its cleaved product, endotrophin (ETP) in tumor-neighboring regions are strongly associated with poor prognosis in HCC patients. Here, we report that the high levels of ETP in injured hepatocytes induce JNK-dependent hepatocyte apoptosis and activate non-parenchymal cells to lead further activation of hepatic inflammation, fibrosis, and apoptosis...
September 23, 2018: Journal of Pathology
Marina Panova-Noeva, Marie A Neu, Susan Eckerle, Claudia Spix, Astrid Schneider, Irene Schmidtmann, Henri M Spronk, Norbert Pfeiffer, Manfred Beutel, Karl J Lackner, Thomas Münzel, Hugo Ten Cate, Hiltrud Merzenich, Jörg Faber, Philipp S Wild
Cardiovascular disease is the most frequent non-malignant cause of morbidity and mortality in adult survivors of childhood or adolescent cancer. Thrombin generation (TG) analysis gives insight in hypercoagulability as an important mechanism linked to cardiovascular risk factors (CVRFs). In 200 individuals, from the cardiac and vascular late sequelae in long-term survivors of childhood cancer study, TG in platelet-rich plasma (PRP) and platelet-free plasma (PFP) at 1pM tissue factor was investigated. Endogenous thrombin potential (ETP) and peak height were the analysed parameters of a TG curve...
September 22, 2018: Clinical Research in Cardiology: Official Journal of the German Cardiac Society
Jerry A Fereiro, Gilad Porat, Tatyana Bendikov, Israel Pecht, Mordechai Sheves, David Cahen
Making biomolecular electronics a reality will require control over charge transport across biomolecules. Here we show that chemical modulation of the coupling between one of the electronic contacts and the biomolecules in a solid-state junction allows controlling electron transport (ETp) across the junction. Employing the protein azurin (Az), we achieve such modulation as follows: Az is covalently bound by Au-S bonding to a lithographically prepared Au electrode (Au-Az). Au nanowires (AuNW) onto which linker molecules, with free carboxylic group, are bound via Au-S bonds serve as top electrode...
October 17, 2018: Journal of the American Chemical Society
Qi Yan, Mingqun Lin, Weiyan Huang, Omid Teymournejad, Jennifer M Johnson, Franklin A Hays, Zhimin Liang, Guangpu Li, Yasuko Rikihisa
Ehrlichia chaffeensis , an obligatory intracellular bacterium, infects monocytes/macrophages by sequestering a regulator of endosomal traffic, the small GTPase RAB5, on its membrane-bound inclusions to avoid routing to host-cell phagolysosomes. How RAB5 is sequestered on ehrlichial inclusions is poorly understood, however. We found that native Ehrlichia translocated factor-2 (Etf-2), a previously predicted effector of the Ehrlichia type IV secretion system, and recombinant Etf-2 (cloned into the Ehrlichia genome) are secreted into the host-cell cytoplasm and localize to ehrlichial inclusions...
September 18, 2018: Proceedings of the National Academy of Sciences of the United States of America
Dajana Lendak, Dunja Mihajlovic, Gorana Mitic, Milan Ubavic, Aleksandra Novakov-Mikic, Jasmina Boban, Snežana Brkic
INTRODUCTION: The aim of this study was to investigate the role of C3 and C4 complement components in prediction of sepsis outcome. The secondary aim was to determine relationship between complement components and other inflammatory parameters, and parameters of hemostasis. METHODS: One-hundred-thirty-seven patients with sepsis (Sepsis-3 criteria) were included in the study. Routine laboratory markers, predictive APACHEII and SOFA scores, concentrations of C3 and C4, activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), fibrinogen, antithrombin (AT), protein C (PC), protein S (PS), endogenous thrombin potential (ETP), thrombomodulin, and D-dimer were available...
October 2018: Thrombosis Research
Omar Valencia-Méndez, Fabián Alejandro Rodríguez-Zaragoza, Luis Eduardo Calderon-Aguilera, Omar Domínguez-Domínguez, Andrés López-Pérez
Gobies are the most diverse marine fish family. Here, we analysed the gamma-diversity (γ-diversity) partitioning of gobiid fishes to evaluate the additive and multiplicative components of α and β-diversity, species replacement and species loss and gain, at four spatial scales: sample units, ecoregions, provinces and realms. The richness of gobies from the realm Eastern Tropical Pacific (ETP) is represented by 87 species. Along latitudinal and longitudinal gradients, we found that the γ-diversity is explained by the β-diversity at both spatial scales, ecoregions and provinces...
2018: PloS One
Oladunni Oluwoye, Bryan Stiles, Maria Monroe-DeVita, Lydia Chwastiak, Jon M McClellan, Dennis Dyck, Leopoldo J Cabassa, Michael G McDonell
OBJECTIVE: This study examined racial and ethnic differences in treatment outcomes among participants in a randomized controlled trial of an intervention for first-episode psychosis called NAVIGATE. METHODS: Secondary data analyses were conducted for participants randomly assigned to usual community care (N=181) and NAVIGATE (N=223). Generalized estimating equations assessed whether race and ethnicity were associated with psychiatric symptoms and service use (medication management, family psychoeducation, and individual therapy) over a 24-month treatment period, accounting for baseline symptoms, duration of untreated psychosis, and insurance status...
August 28, 2018: Psychiatric Services: a Journal of the American Psychiatric Association
Jing Li, Yaru Zhang, Bruno Da Silva Sil Dos Santos, Feng Wang, Yuyong Ma, Christian Perez, Yanling Yang, Junmin Peng, Seth M Cohen, Tsui-Fen Chou, Stephen T Hilton, Raymond J Deshaies
The 26S proteasome is the major proteolytic machine for breaking down cytosolic and nuclear proteins in eukaryotes. Due to the lack of a suitable assay, it is difficult to measure routinely and quantitatively the breakdown of proteins by the 26S proteasome in vitro. In the present study, we developed an assay to monitor proteasome-mediated protein degradation. Using this assay, we discovered that epidithiodiketopiperazine (ETPs) blocked the degradation of our model substrate in vitro. Further characterization revealed that ETPs inhibited proteasome function by targeting the essential proteasomal deubiquitinase Rpn11 (POH1/PSMD14)...
August 22, 2018: Cell Chemical Biology
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