Zhiqiang Duan, Yafeng Liang, Jialei Sun, Hongjin Zheng, Tong Lin, Pengyu Luo, Mengge Wang, Ruiheng Liu, Ying Chen, Shuhua Guo, Nannan Jia, Hongtao Xie, Meili Zhou, Minghui Xia, Kaijun Zhao, Shuhui Wang, Na Liu, Yongling Jia, Wei Si, Qitong Chen, Yechun Hong, Ruilin Tian, Jian-Kang Zhu
The type V-I CRISPR-Cas system is becoming increasingly more attractive for genome editing. However, natural nucleases of this system often exhibit low efficiency, limiting their application. Here, we used structure-guided rational design and protein engineering to optimize an uncharacterized Cas12i nuclease, Cas12i3. As a result, we developed Cas-SF01, a Cas12i3 variant that exhibits significantly improved gene editing activity in mammalian cells. Cas-SF01 shows comparable or superior editing performance compared to SpCas9 and other Cas12 nucleases...
March 4, 2024: The innovation