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Sensitized Fluorescence

Vera S Donnenberg, Mirko Corselli, Daniel P Normolle, E Michael Meyer, Albert D Donnenberg
Flow cytometry is often performed on adherent cells or solid tissues that have been released from their growth substrate or disaggregated by enzymatic digestion. Although detection of strongly expressed cell surface proteins following such procedures indicates that many survive treatment with proteolytic enzymes, applications such as cell surface proteomics involve assessment of the expression of more than 200 proteins and it is important to know how to interpret negative results. To address this problem, we performed flow cytometry-based cell surface proteomic analysis on two non-adherent cell lines, THP1 and K562, after mock and authentic trypsin treatment, according to a widely used protocol to remove adherent cells (0...
August 14, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Fahad M Aldakheel, Jane E Bourke, Paul S Thomas, Melanie C Matheson, Michael J Abramson, Garun S Hamilton, Caroline J Lodge, Bruce R Thompson, Haydn E Walters, Katrina J Allen, Bircan Erbas, Jennifer L Perret, Shyamali C Dharmage, Adrian J Lowe
BACKGROUND: Asthma and allergic diseases are heterogeneous. Measurement of biomarkers in exhaled breath condensate (EBC) may help to discriminate between different phenotypes and may assist with clinical prognostication. OBJECTIVES: We aimed to assess associations between total nitric oxide products (NOx ) in EBC and different allergic phenotypes and lung function in young and middle-aged adults. METHODS: Cross-sectional analyses were nested within two Australian longitudinal studies, the Melbourne Atopy Cohort Study (MACS, mean age 17...
August 14, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Emily Langron, Stella Prins, Paola Vergani
BACKGROUND AND PURPOSE: Cystic fibrosis (CF) is a debilitating hereditary disease caused by mutations in the CFTR gene, which encodes an anion channel. WT-CFTR gating is a non-equilibrium process. After ATP binding, CFTR enters a stable open state (O1 ). ATP hydrolysis leads it to a short-lived post-hydrolytic open state (O2 ), from which channels close. Here we use mutations to probe the mechanism of VX-770, the first compound directly targeting the CFTR protein approved for treatment of CF...
August 14, 2018: British Journal of Pharmacology
Kuo-Hwa Wang, Yun-Ming Wang, Li-Hsuan Chiu, Tze-Chien Chen, Yu-Hui Tsai, Chun S Zuo, Kuan-Chou Chen, Chun Austin Changou, Wen-Fu T Lai
[This corrects the article DOI: 10.1371/journal.pone.0192047.].
2018: PloS One
Shang Dai, Ye Jin, Tao Li, Yulan Weng, Xiaolin Xu, Genlin Zhang, Jiulong Li, Renjiang Pang, Bing Tian, Yuejin Hua
Iron acquisition by bacteria is well studied, but iron export from bacteria is less understood. Herein, we identified dr1440 with a P-type ATPase motif as a potential exporter of iron from Deinococcus radiodurans, a bacterium known for its extreme resistance to radiation and oxidants. The DR1440 was located in cell membrane as demonstrated by fluorescence labelling analysis. Mutation of dr1440 resulted in cellular accumulation of iron ions, and expression level of dr1440 was up-regulated significantly under iron ion or hydrogen peroxide stress in the wild-type strain, implicating DR1440 as a potential iron efflux protein...
2018: PloS One
Lu Zhao, Jiashan Mao, Bin Jiang, Xiantao Wei, Yonghu Chen, Min Yin
A new strategy of optical thermometry is realized by long persistent luminescence phosphor SrAl2 O4 :Eu2+ , Dy3+ , Tb3+ (SAEDT). Under different temperatures, SAEDT shows bright afterglow emissions after cessation of the UV excitation. The afterglow color of the SAEDT sample is blue at 60 K and gradually changed into green at 240 K. The normalized afterglow spectra at different temperatures give a dramatic change of fluorescence intensity ratio between the blue band and the green band. Not only has this material exhibited a high absolute sensitivity and relative sensitivity for temperature sensing, but it also has the great advantage of eliminating the heating effect due to avoidance of real-time direct excitation...
August 15, 2018: Optics Letters
Seung Yup Lee, Mary-Ann Mycek
We present a hybrid Monte Carlo simulation method with geometrical ray tracing (hMC-GRT) to model fluorescence excitation and detection in turbid media by optical imaging or spectroscopy systems employing a variety of optical components. hMC-GRT computational verification was achieved via reflectance and fluorescence simulations on epithelial tissue models in comparison with a standard Monte Carlo code. The mean difference between the two simulations was less than 5%. hMC-GRT experimental verification employed depth-sensitive steady-state fluorescence measurements using an aspherical lens on two-layered tissue phantoms...
August 15, 2018: Optics Letters
Vladimir M Kushnir, Daniel K Mullady, Koushik Das, Gabriel Lang, Thomas G Hollander, Faris M Murad, Sara A Jackson, Nicole A Toney, Sydney D Finkelstein, Steven A Edmundowicz
BACKGROUND: Routine cytology of biliary stricture brushings obtained during endoscopic retrograde cholangiopancreatography (ERCP) has suboptimal sensitivity for malignancy. We compared the individual and combined ability of cytology, fluorescence in situ hybridization (FISH) analysis and PCR-based mutation profiling (MP) to detect malignancy in standard biliary brushings. METHODS: We performed a prospective study of patients undergoing ERCP using histology or 1 year follow-up to determine patient outcomes...
August 13, 2018: Journal of Clinical Gastroenterology
Jian Sun, Fude Feng
We reported a near-infrared biosensor that features a dihydromethylene blue and an S-alkyl thiocarbamate linker to detect hypochlorous acid in a drastic fluorescence turn-on response. We achieved high sensitivity at the nanomolar level and high selectivity that resolves the interference issue with mercury ions and other transition metal ions. We demonstrated the response mechanism by analysing the released segments, and investigated the imaging capability to detect both exogenous and endogenous hypochlorous acid in living cells...
August 14, 2018: Analyst
Sushil Kumar Dwivedi, Ramesh C Gupta, Priyanka Srivastava, Priya Singh, Biplob Koch, Biswajit Maiti, Arvind Misra
The present work describes a new PET based molecular probe in which naphthalimide (NPI) and anthracene (AN) chromophores are linked through a molecular bridge of piperazine and triazole units by Click reaction. A typical meaningful structural variation has made the present probe highly selective for Cr3+ ion (LOD, 5.567x10-8 M) that displayed enhanced, "turn - on" emission (due to PET-Off photophysical mechanism) and naked-eye sensitive bright green color fluorescence in the environment of interfering and competitive ions, in Tris-HCl buffer...
August 14, 2018: Analytical Chemistry
Ehsan Soheyli, Reza Sahraei, Golamreza Nabiyouni, Fereshteh Nazari, Reza Tabaraki, Behnaz Ghaemi
In this paper, an aqueous-based approach is introduced for facile, fast, and green synthesis of gradient-alloyed Fe-doped ZnSe(S)@ZnSe(S) core:shell QDs with intense and stable emission. Co-utilizing of co-nucleation and growth doping strategies, along with systematic optimization of emission intensity, provide a well-controllable/general method to achieve internally doped-quantum dots (d-dots) with intense emission. Results indicated that the alloyed ZnSe(S)@ZnSe(S) core:shell QDs have a gradient structure which consists of the Se-rich core and the S-rich shell...
August 14, 2018: Nanotechnology
Akash Gulyani, Nilanjan Dey, Santanu Bhattacharya
Despite decades-long extensive research, probes that provide a comprehensive description of the lipid membrane microenvironment are still lacking. We report here a 'smart' pyrene-terpyridine probe for multiparametric sensing of lipid membranes; wherein the complexity of the local microenvironment can be described by distinct features of the probe fluorescence. The self-assembly of the probe molecules in phospholipid bilayers is sensitive to membrane order and phase state. The self-assembled probes show a unique emission, influenced by dye-dye interactions and excited state charge transfer...
August 14, 2018: Chemistry: a European Journal
Mengke Wang, Dandan Su, Guannan Wang, Xingguang Su
In this work, a facile, label-free, and sensitive fluorometric strategy for detection of trypsin and its inhibitor was established on the basis of the fluorescence resonance energy transfer (FRET) between mercaptoundecanoic acid functionalized gold nanoclusters (AuNCs) and gold nanoparticles (AuNPs) via protamine as a bridge. Protamine can trigger the aggregation of AuNPs and link AuNCs with aggregated AuNPs through electrostatic interaction. Compared with monodisperse AuNPs, the UV-vis absorption band of aggregated AuNPs overlapped considerably with the emission spectrum of AuNCs...
August 13, 2018: Analytical and Bioanalytical Chemistry
Na Liu, Kai Xu, Liquan Liu, Xin Chen, Yufan Zou, Xianjin Xiao
A star-shaped fluorescent DNA probe (S-probe) is described that can recognize target DNA and discriminate it from interfering DNA via strand displacement including branch migration and toehold exchange. The artificially designed S-probe does not harm the strand displacement while it allows the fluorescently labelled strand and the quencher-labelled strand to be shared among different S-probes targeting different genetic variations. Generally, multiplexed detection of different MT/WT pairs requires different fluorophore-labelled and quencher-labelled strands...
August 13, 2018: Mikrochimica Acta
Shan Wang, Ruiqing Liu, Chenchen Li
In the presence of Hg2+ , a fluorescence resonance energy transfer (FRET) system was constructed between CdSe quantum dots (QDs) (donor) and g-C3 N4 (receptors). Nanocomposites of g-C3 N4 supported by CdSe QDs (CdSe QDs/g-C3 N4 nanosheets) were fabricated through an electrostatic interaction route in an aqueous solution. The nanocomposites were characterized by X-ray photoelectron spectroscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and transmission electron microscopy. Results showed that the g-C3 N4 nanosheets were decorated randomly by CdSe QDs, with average diameter of approximately 7 nm...
August 13, 2018: Nanoscale Research Letters
Yong-Peng Li, Shu-Ni Li, Yu-Cheng Jiang, Man-Cheng Hu, Quan-Guo Zhai
Presented herein is a magnesium-organic framework (SNNU-88) incorporated with active hydroquinone groups, which exhibits not only remarkable semiconductor sensing for traces of ammonia vapor (5-100 ppm), but also extra-high fluorescence response to liquid NH3·H2O through an unusual turn-off (0-1.5 ppm) and turn-on (3.0-100 ppm) luminescence sensing mechanism at room temperature. Such bifunctional quantitative ammonia detection together with the extraordinary sensitivity and selectivity, fast response, and good reusability makes SNNU-88 one of the most powerful ammonia sensors...
August 14, 2018: Chemical Communications: Chem Comm
Junhou Hui, Yin Gu, Yuanshou Zhu, Yanjing Chen, Shu-Juan Guo, Sheng-Ce Tao, Yan Zhang, Peng Liu
A pipette-actuated capillary array comb (PAAC) system operated on a smartphone-based hand-held device has been successfully developed for the multiplex detection of bacteria in a "sample-to-answer" manner. The PAAC consists of eight open capillaries inserted into a cylindrical plastic base with a piece of chitosan-modified glass filter paper embedded in each capillary. During the sample preparation, a PAAC was mounted into a 1 mL pipette tip with an enlarged opening and was operated with a 1 mL pipette for liquid handling...
August 14, 2018: Lab on a Chip
Xiangqi Liu, Zhenzhen Zhang, Binbin Zhang, Yijie Zheng, Chao Zheng, Baihui Liu, Shan Zheng, Kuiran Dong, Rui Dong
Detecting circulating tumor cells (CTCs) has proven valuable for evaluating the prognosis of cancer patients and for studying the mechanisms of treatment resistance. Owing to the lack of universal and specific tumor markers for neuroblastoma (NB), in this prospective study, we adopted an EpCAM-independent method to detect CTCs in the peripheral blood of NB patients. We used an EpCAM-independent assay to delete leukocytes and to enrich the CTCs. CTCs were identified by immunostaining of CD45, DAPI and DNA fluorescence in situ hybridization (FISH) of the centromere of chromosome 8 probe (CEP8)...
August 10, 2018: EBioMedicine
Kazunori Sugiura, Yuta Nishimaki, Mikito Owa, Toru Hisabori, Ken-Ichi Wakabayashi
Cellular reducing-oxidizing (redox) potential is mainly determined by the concentration ratio between reduced and oxidized glutathiones. It is normally kept at a moderately reduced state but affected to some extent by metabolic activities such as respiration and/or photosynthesis. Changes in redox potential induce many cellular activities collectively called redox responses. For an understanding of the dynamics of the cellular redox responses, redox potential must be accurately assessed in vivo. In this study, we developed a method to measure the in vivo redox potential in the green alga Chlamydomonas reinhardtii, using Oba-Qc, a recently developed redox-monitoring protein...
August 10, 2018: Biochemical and Biophysical Research Communications
Nur Fadhila Fadzil, Alina Wagiran, Faezah Mohd Salleh, Shamsiah Abdullah, Nur Hazwani Mohd Izham
The present study demonstrated High Resolution Melting (HRM) analysis combined with DNA barcode (Bar-HRM) as a fast and highly sensitive technique for detecting adulterants in Eurycoma longifolia commercial herbal products. Targeting the DNA barcoding of the chloroplastic region-ribulose biphosphate carboxylase large chain (rbcL) and the nuclear ribosomal region- internal transcribed spacer 2 (ITS2), PCR amplification and HRM analysis using saturated Eva green dye as the source of fluorescence signals, was accomplished by employing a real-time cycler...
August 12, 2018: Genes
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