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Sensitized Fluorescence

Zheng Guo, Xiaohua Zhu, Shigong Wang, Chunyang Lei, Yan Huang, Zhou Nie, Shouzhuo Yao
As an emerging two-dimensional material, MXenes have attracted much attention due to their unique physicochemical properties, but their application in biosensing has been lagging far behind because of their poor salt tolerance. Herein, a titanium carbide MXene quantum dot (Ti3C2 QD)-based fluorescent probe for the alkaline phosphatase (ALP) activity assay and embryonic stem cell (ESC) identification was developed by taking advantage of the inner filter effect (IFE). Ti3C2 QDs with ∼4.2 nm in diameter were prepared from Ti3C2 MXenes by hydrothermal treatment, exhibiting excellent salt tolerance, anti-photobleaching and dispersion stability in aqueous solution...
October 16, 2018: Nanoscale
Arathyram Ramachandra Kurup Sasikala, Afeesh Rajan Unnithan, Reju George Thomas, Tumurbaatar Batgerel, Yong Yeon Jeong, Chan Hee Park, Cheol Sang Kim
In order to meet the unmet medical needs for effective cancer treatment, multifunctional nanocarriers based on iron oxide nanoparticles hold tremendous promise. Here we report a superparamagnetic iron oxide nanoparticles based hexa-functional nanosystem for synergistic cancer theranostic applications by offering active tumour targeting, accumulation and complementary imaging capability by combining magnetic resonance imaging as well as near-infrared fluorescence, magnetophotothermia and chemotherapy. The uniquely designed nanosystem exhibited a paramount increase in the antitumour efficacy through the simultaneous application of multiple thermal effects called magnetophotothermia, which outweighed the therapeutic efficacy of the current thermo-chemo therapies or stand-alone therapies...
October 16, 2018: Nanoscale
Hu Ou, Jing Chen, Pengfei Gao, Gangfeng Li, Shijie Du, Haiyan Fu, Qiong Shi, Lu Xu
BACKGROUND: This paper investigated the feasibility of data fusion of NIR and fluorescence spectroscopy for rapid analysis of cheap vegetable oils in Chinese Camellia Oleifera Abel. (COA) oil. Because practical frauds usually involve adulterations of multiple known and unknown cheap oils, traditional analytical methods aimed at detecting one or more known adulterants are insufficient to identify adulterated COA oil. Therefore, untargeted analysis was performed by developing class models of pure COA oil using robust one-class partial least squares (OCPLS)...
October 16, 2018: Journal of the Science of Food and Agriculture
Youli Qiu, Yu Li
A highly sensitive method for the detection of phthalate acid ester (PAE) derivatives by fluorescence spectroscopy based on a double-substitution modification is reported. The fluorescence intensities of 18 PAE derivatives and the template PAEs after docking with the protein bovine serum albumin (BSA) are compared; the fluorescence intensities of the PAE derivatives increase significantly (28.20 to 110.00 times) with high sensitivities, but the functionalities (stability and insulation) are not affected. The persistence, mobility, and toxicity of the PAE derivatives are reduced by varying degrees, and their amounts of bioaccumulation are not significantly changed, indicating that the derivatives are more environmentally friendly...
October 15, 2018: Environmental Science and Pollution Research International
Mepur H Ravindranath, Edward J Filippone, Grace Mahowald, Carly Callender, Adarsh Babu, Susan Saidman, Soldano Ferrone
The accurate measurement of anti-HLA alloantibodies in transplant candidates is required for determining the degree of sensitization and for the listing of unacceptable antigens for organ allocation. Both the configuration of the HLA molecules coated on the beads and the nature of detection antibodies may impede assessment of the presence and strength of anti-HLA IgG- with the Luminex single-antigen-bead assay. Sera antibodies of the end-stage renal disease patients were compared using LIFECODES (LC) and LABScreen (LS) beadsets monitored with polyclonal-Fab (IgHPolyFab) and monoclonal-IgG (FcMonoIgG) second antibodies...
October 16, 2018: Immunologic Research
Dana Elbers, Alexander Scholten, Karl-Wilhelm Koch
Primary steps in vertebrate vision occur in rod and cone cells of the retina and require precise molecular switches in excitation, recovery, and adaptation. In particular, recovery of the photoresponse and light adaptation processes are under control of neuronal Ca2+ sensor (NCS) proteins. Among them, the Ca2+ sensor recoverin undergoes a pronounced Ca2+ -dependent conformational change, a prototypical so-called Ca2+ -myristoyl switch, which allows selective targeting of G protein-coupled receptor kinase. Zebrafish ( Danio rerio ) has gained attention as a model organism in vision research...
2018: Frontiers in Molecular Neuroscience
Yuanyuan Zheng, Hong Zhang, Yuping Hu, Lu Bai, Jingyi Xue
Background: Myocardial infarction (MI) is a leading cause of death worldwide. Therefore, nanoparticles that applied for specific diagnosis of the infarcted area and/or local myocardial delivery of therapeutic agents, are highly desired. Materials and methods: Herein, we developed the MnO-based nanoparticles, with magnetic resonance (MR) and near-infrared fluorescence imaging modalities as an MR imaging contrast agent and potential drug vehicle for the detection and treatment of MI...
2018: International Journal of Nanomedicine
Ashley Mooneyham, Yoshie Iizuka, Qing Yang, Courtney Coombes, Mark McClellan, Vijayalakshmi Shridhar, Edith Emmings, Mihir Shetty, Liqiang Chen, Teng Ai, Joyce Meints, Michael K Lee, Melissa Gardner, Martina Bazzaro
UNC-45A, a highly conserved member of the UCS (UNC45A/CRO1/SHE4P) protein family of co-chaperones, plays an important role in regulating cytoskeletal-associated functions in invertebrates and mammalian cells including cytokinesis, exocytosis, cell motility, and neuronal development. Here, for the first time UNC-45A is demonstrated to function as a mitotic-spindle-associated protein that destabilizes microtubules (MTs) activity. Using in vitro biophysical reconstitution and total internal reflection fluorescence (TIRF) microscopy analysis, reveal that UNC-45A directly binds to taxol-stabilized MTs in the absence of any additional cellular co-factors or other microtubule-associated proteins (MAPs) and acts as an ATP-independent microtubule destabilizer...
October 15, 2018: Molecular Cancer Research: MCR
Zhengyi Qu, Weidan Na, Yixin Nie, Xingguang Su
In this paper, we developed a sensitive sensor for phytic acid (PA) and hydrogen peroxide (H2 O2 ) detection based on glutathione-functionalized graphene quantum dots (GQDs@GSH). The fluorescence of GQDs@GSH was found to be effectively quenched by Fe3+ ions via photo-induced electron transfer (PET) process. Upon the addition of PA to GQDs@GSH/Fe3+ system, the fluorescence of GQDs@GSH was significantly restored due to the strong chelating and reducing ability of PA, Fe3+ ions could be reduced to Fe2+ ions by PA and formed PA/Fe2+ complex...
December 18, 2018: Analytica Chimica Acta
M Amin Farahmand Nejad, Forough Ghasemi, M Reza Hormozi-Nezhad
Owing to its dual role as a hormone and neurotransmitter, norepinephrine (NE) detection is of great significance to biomedical diagnosis. In the present work, we have explored intense green fluorescence of poly (norepinephrine) (PNE) nanoparticles synthesized by oxidizing NE in alkaline condition, in combination with red fluorescent bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) for naked-eye detection of NE. The effect of sodium hydroxide on the emission behavior of NE was studied. The surface morphology and optical properties of PNE nanoparticles were characterized by UV-Vis, fluorescence, FTIR, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) techniques...
December 18, 2018: Analytica Chimica Acta
Li Yan Chan, Joshua Ding Wei Teo, Kevin Shyong-Wei Tan, Keitaro Sou, Wei Lek Kwan, Chi-Lik Ken Lee
Chloroquine was among the first of several effective drug treatments against malaria until the onset of chloroquine resistance. In light of diminished clinical efficacy of chloroquine as an antimalarial therapeutic, there is potential in efforts to adapt chloroquine for other clinical applications, such as in combination therapies and in diagnostics. In this context, we designed and synthesized a novel asymmetrical squaraine dye coupled with chloroquine (SQR1-CQ). In this study, SQR1-CQ was used to label live Plasmodium falciparum ( P...
October 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Qiaona Xue, Rui Wang, Wenjing Xu, Jiangtao Wang, Liju Tan
The allelopathic effects of extracts isolated from the culture filtrate of diatom Phaeodactylum triconutum Bohlin on typical marine microalgae Prorocentrum donghaiense Lu and Dunaliella salina Teodoresco were investigated by determining different physiological and biochemical parameters, such as growth rate, membrane systems and esterase activity under controlled laboratory conditions. The growth of P. donghaiense was significantly inhibited immediately after exposure to the allelochemicals, while the algae density of D...
October 9, 2018: Aquatic Toxicology
Nannan Wang, Yue Wang, Jinhua Gao, Xin Ji, Jinling He, Jian Zhang, Weili Zhao
Biological thiols, especially low molecular weight thiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play a pivotal role in physiological and pathological systems. Thus, the detection of biothiols is highly important for early diagnosis of diseases and evaluation of disease progression. Herein, we developed a highly selective and sensitive ratiometric fluorescent 8-Cl BODIPY-based probe with high fluorescence quantum yields. The probe displayed a sensitive response to Cys and Hcy over other biothiols, which can be visualized colorimetrically and/or fluorescently...
October 15, 2018: Analyst
Jung-Shen B Tai, Ivan I Smalyukh
Extending the optical imaging of director structures in liquid crystals (LCs) beyond the diffraction limit is poised to provide insights into previously elusive LC physics at the nanoscale. Here, we develop and characterize super-resolution stimulated emission depletion microscopy with molecular orientation sensitivity and apply it to reveal spatially localized director structures in chiral nematic and smectic LCs. As examples, we demonstrate director imaging and reconstruction of nanoscale LC configurations, including solitonic Bloch walls and two-dimensional skyrmions, both observed in sub-micrometer-thick strongly confined LC films, and focal conic domains in smectic LCs...
October 15, 2018: Optics Letters
Gregor Langer, Thomas Berer
In this Letter, we theoretically describe photoacoustic signal generation of molecules, for which triplet relaxation can be neglected, by considering the excited state lifetime, the fluorescence quantum yield, and the fast vibrational relaxation. We show that the phase response of the photoacoustic signal can be exploited to determine the excited state lifetime of dark molecules. For fluorescent molecules, the phase response can be used to determine the fluorescence quantum yield directly without the need of reference samples...
October 15, 2018: Optics Letters
Jing Peng, Ivan J Santiago, Matthew Y Pecot
Recent improvements in the sensitivity of next generation sequencing have facilitated the application of transcriptomic and genomic analyses to small numbers of cells. Utilizing this technology to study development in the Drosophila visual system, which boasts a wealth of cell type-specific genetic tools, provides a powerful approach for addressing the molecular basis of development with precise cellular resolution. For such an approach to be feasible, it is crucial to have the capacity to reliably and efficiently purify cells present at low abundance within the brain...
September 26, 2018: Journal of Visualized Experiments: JoVE
Michael Ronzetti, Bolormaa Baljinnyam, Adam Yasgar, Anton Simeonov
Drug plasma protein binding remains highly relevant to research and drug development, making the assessment and profiling of compound affinity to plasma proteins essential to drug discovery efforts. Although there are a number of fully-characterized methods, they lack the throughput to handle large numbers of compounds. As the evaluation of adsorption, distribution, metabolism, and excretion is addressed earlier in the drug development timeline, the need for higher-throughput methods has grown. Areas Covered: This review will highlight recent developments on methods for profiling drug plasma binding, with an emphasis on fluorescent probes and emerging high-throughput methodologies...
October 15, 2018: Expert Opinion on Drug Discovery
Chenlin Yang, Rui Hu, Qian Li, Shuang Li, Junfeng Xiang, Xudong Guo, Shuangqing Wang, Yi Zeng, Yi Li, Guoqiang Yang
G-quadruplexes (G4s) are unique four-stranded nucleic acid secondary structures formed by G-rich nucleic acid sequences which are prevalent in gene promoter and telomere regions and deemed to play essential roles in many biological and pathological processes. Although attentions to G4s have been paid for nearly 40 years, G4 selectivity and its topology discrimination in cells is still pending. Small fluorescence molecules are emerging as a versatile tool of interrogation of cellular features in vivo. Herein, a new class of bis(4-aminobenzylidene)acetone derivatives GD1, GD2, and GD3 with excellent environment-sensitive emission properties were developed and used for fluorescent detection of G4s...
September 30, 2018: ACS Omega
João L Lagarto, Benjamin T Dyer, Clifford B Talbot, Nicholas S Peters, Paul M W French, Alexander R Lyon, Chris Dunsby
Autofluorescence spectroscopy is a promising label-free approach to characterize biological samples with demonstrated potential to report structural and biochemical alterations in tissues in a number of clinical applications. We report a characterization of the ex vivo autofluorescence fingerprint of cardiac tissue, exploiting a Langendorff-perfused isolated rat heart model to induce physiological insults to the heart, with a view to understanding how metabolic alterations affect the autofluorescence signals...
October 1, 2018: Biomedical Optics Express
Yeye Xu, Guorong Wang, Xibo Wang, Jie Yu, Jian Wang, Zeyu Zhang, Rui Li
The sensitivity of soy protein isolate (SPI) to trypsin was characterized by its flexibility. The effects of different homogenization conditions on soy protein isolate flexibility and emulsifying properties were investigated. Set the homogenization pressure was 120 MPa (megapascal) and the homogenous number of times is 0-4 times, the flexibility increases with the increase of the homogenization times (0-3 times), the change trend of flexibility is not obvious (3-4 times). When the homogenization times was 0-3 times, the emulsifying activity increases, and the emulsifying activity was the strongest at 3 times, after homogenization 3 times, the change trend of emulsifying activity is not obvious, the trend of emulsification stability and emulsification activity were similar...
October 2018: Food Science and Biotechnology
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