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https://www.readbyqxmd.com/read/30105182/genetic-diversity-of-chilli-veinal-mottle-virus-infecting-different-chilli-landraces-in-north-east-india-indicates-the-possibility-of-transboundary-movement-of-virus
#1
Rakesh Sanabam, Ng Taibangnganbi Chanu, Susheel Kumar Sharma, S S Roy, M A Ansari, N Prakash
Diverse chilli genetic resources of North East India occupy a unique niche in the Nation's chilli gene pool. Widely cultivated chilli landraces (King chilli: Capsicum chinense , bird eye chilli: Capsicum frutescens and Capsicum annuum ) of North East India in general and Manipur in particular suffer from decline complex due to high incidence of viral diseases. With this background and the known prominent distribution of Chilli veinal mottle virus (ChiVMV) in Asian region, we studied its prevalence and association with diverse symptoms of chilli landraces...
August 2018: 3 Biotech
https://www.readbyqxmd.com/read/30104765/clinical-manifestations-and-ophthalmic-outcomes-of-ocular-syphilis-at-a-time-of-re-emergence-of-the-systemic-infection
#2
João M Furtado, Tiago E Arantes, Heloisa Nascimento, Daniel V Vasconcelos-Santos, Natalia Nogueira, Rafael de Pinho Queiroz, Luana P Brandão, Thaís Bastos, Ricardo Martinelli, Rodrigo C Santana, Cristina Muccioli, Rubens Belfort, Justine R Smith
Recent reports from different world regions suggest ocular syphilis is re-emerging, in parallel with an increasing incidence of the systemic infection globally. We conducted a large observational study of 127 persons consecutively treated for ocular syphilis at public medical centers in Brazil over a 2.5-year period ending July 2015. Of 104 individuals serologically tested for human immunodeficiency virus (HIV), 34.6% were positive. Ophthalmological evaluations included measurement of Snellen visual acuity and intraocular pressure, and assessment of inflammation by slit lamp examination and dilated posterior eye examination...
August 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30104566/visual-and-modular-detection-of-pathogen-nucleic-acids-with-enzyme-dna-molecular-complexes
#3
Nicholas R Y Ho, Geok Soon Lim, Noah R Sundah, Diana Lim, Tze Ping Loh, Huilin Shao
Rapid, visual detection of pathogen nucleic acids has broad applications in infection management. Here we present a modular detection platform, termed enzyme-assisted nanocomplexes for visual identification of nucleic acids (enVision). The system consists of an integrated circuit of enzyme-DNA nanostructures, which function as independent recognition and signaling elements, for direct and versatile detection of pathogen nucleic acids from infected cells. The built-in enzymatic cascades produce a rapid color readout for the naked eye; the assay is thus fast (<2 h), sensitive (<10 amol), and readily quantified with smartphones...
August 13, 2018: Nature Communications
https://www.readbyqxmd.com/read/30102483/a-novel-homozygous-w99g-mutation-in-cldn-16-gene-causing-familial-hypomagnesemic-hypercalciuric-nephrocalcinosis-in-turkish-siblings
#4
Caner Alparslan, Elif Perihan Öncel, Sinem Akbay, Demet Alaygut, Fatma Mutlubaş, Mansur Tatlı, Martin Konrad, Önder Yavaşcan, Belde Kasap-Demir
Alparslan C, Öncel EP, Akbay S, Alaygut D, Mutlubaş F, Tatlı M, Konrad M, Yavaşcan Ö, Kasap-Demir B. A novel homozygous W99G mutation in CLDN-16 gene causing familial hypomagnesemic hypercalciuric nephrocalcinosis in Turkish siblings. Turk J Pediatr 2018; 60: 76-80. Familial hypomagnesemic hypercalciuric nephrocalcinosis (FHHNC) (OMIM: 248250) is characterized by hypomagnesemia, hypercalciuria and nephrocalcinosis. FHHNC inevitably progresses to end-stage renal disease in decades. Mutations in CLDN-16 and CLDN-19 genes are associated with disrupted magnesium handling in the thick ascending limp of Henle`s loop...
2018: Turkish Journal of Pediatrics
https://www.readbyqxmd.com/read/30099802/bilateral-intra-orbital-masses-in-a-child
#5
Parikshaa Gupta, Gurwinder Kaur, Arvind Rajwanshi, Sandeep Bansal, Rajeev Kamble, R Ranjini
16-year-old male child. Presented with bilateral proptosis (more on right than left) over the last 8 years, which was painless and gradually increasing in size. He had slight blurring of vision in right eye with restriction of eye movements. In addition, he had difficulty in breathing from nose. There was no history of fever, fatigue, weight loss, night sweats or joint pains. The clinical suspicion was of a lymphoma or an invasive fungal infection. On examination, there was no significant cervical, axillary or inguinal lymphadenopathy or hepatosplenomegaly...
August 12, 2018: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/30099393/village-integrated-eye-worker-trial-view-rationale-and-design-of-a-cluster-randomised-trial-to-prevent-corneal-ulcers-in-resource-limited-settings
#6
Kieran S O'Brien, Raghunandan Byanju, Ram Prasad Kandel, Bimal Poudyal, Mariya Gautam, John A Gonzales, Travis C Porco, John P Whitcher, Muthiah Srinivasan, Madan Upadhyay, Thomas M Lietman, Jeremy D Keenan
INTRODUCTION: Corneal opacity is a leading cause of blindness worldwide. In resource-limited settings, untreated traumatic corneal abrasions may result in infection and ultimately, opacity. Although antimicrobial treatment of corneal ulcers may successfully cure infections, the scarring that accompanies the resolution of infection can still result in visual impairment. Prevention may be the optimal approach for reducing corneal blindness. Studies have employed community health workers to provide prompt administration of antimicrobials after corneal abrasions to prevent infections, but these studies were not designed to determine the effectiveness of such a programme...
August 10, 2018: BMJ Open
https://www.readbyqxmd.com/read/30098351/the-asia-cornea-society-infectious-keratitis-study-a-prospective-multicenter-study-of-infectious-keratitis-in-asia
#7
Wei-Boon Khor, Venkatesh N Prajna, Prashant Garg, Jodhbir S Mehta, Lixin Xie, Zuguo Liu, Ma Dominga B Padilla, Choun-Ki Joo, Yoshitsugu Inoue, Panida Goseyarakwong, Fung-Rong Hu, Kohji Nishida, Shigeru Kinoshita, Vilavun Puangsricharern, Ai-Ling Tan, Roger Beuerman, Alvin Young, Namrata Sharma, Benjamin Haaland, Francis S Mah, Elmer Y Tu, Fiona J Stapleton, Richard L Abbott, Donald Tiang-Hwee Tan
PURPOSE: To survey the demographics, risk factors, microbiology and outcomes for infectious keratitis in Asia. DESIGN: Prospective, non-randomized clinical study METHODS: Thirteen study centers and 30 sub-centers recruited consecutive subjects over 12 - 18 months, and performed standardized data collection. A microbiological protocol standardized the processing and reporting of all isolates. Treatment of the infectious keratitis was decided by the managing ophthalmologist...
August 8, 2018: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/30098249/-analysis-of-clinical-manifestation-and-genetic-mutation-in-a-child-with-x-linked-chondrodysplasia-punctata-2
#8
Guoying Chang, Yunfang Zhou, Lei Yin, Longjun Gu, Daming Ying, Huijin Chen, Xiumin Wang, Jian Wang
OBJECTIVE: To analyze clinical manifestations and genetic mutation in a child with severe short stature and other malformations. METHODS: The child has undergone history taking and physical examination. Genome DNA was extracted from peripheral blood samples of the proband and her family members. Candidate genes were captured with Agilent SureSelect and sequenced on an Illumina platform. Suspected mutation was verified by Sanger sequencing. RESULTS: The patient, a six-year-and-10-month old girl, presented with non-symmetrical short stature, dysmorphism, abnormalities of limbs and spine, amblyopia of left eye, and cataract of right eye, in addition with frequent respiratory infection and micturition...
August 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/30095666/dengue-fever-associated-necrotizing-scleritis-a-case-report-with-long-term-follow-up
#9
Koju Kamoi, Manabu Mochizuki, Kyoko Ohno-Matsui
RATIONALE: Dengue fever is a notable emerging infectious disease that is now seen worldwide, with an estimated incidence of approximately 390 million cases per year. Although ocular complications are uncommon among dengue fever-infected patients, caution is needed to prevent vision loss. Here we report a potentially serious sight-threatening complication of dengue fever, dengue fever-associated necrotizing scleritis. PATIENT CONCERNS AND DIAGNOSIS: After being bitten by mosquitoes, a 60-year-old Japanese female exhibited positive serologic tests of immunoglobulin M and G enzyme immunoassays for dengue viral infection along with a decrease of leukocytes and platelets...
August 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/30094541/contact-lens-related-polymicrobial-keratitis-acanthamoeba-spp-genotype-t4-and-candida-albicans
#10
Maria Luiza Carneiro Buchele, Débora Borgert Wopereis, Fabiana Casara, Jefferson Peres de Macedo, Marilise Brittes Rott, Fabíola Branco Filippin Monteiro, Maria Luiza Bazzo, Fernando Dos Reis Spada, Jairo Ivo Dos Santos, Karin Silva Caumo
A 31-year-old female daily user of contact lenses sought medical attention, reporting blurred vision and irritation of the left eye. Slit-lamp examination revealed hyperemia and an irregular corneal epithelium surface, and empirical treatment was started. A corneal scrape was obtained and examined for the presence of fungi, bacteria, and Acanthamoeba spp. The results of the microbial culture revealed growth of Acanthamoeba spp. and Candida albicans. The Acanthamoeba isolate was characterized by cyst morphology as belonging to group II according to Pussard and Pons...
August 10, 2018: Parasitology Research
https://www.readbyqxmd.com/read/30094156/post-primary-pulmonary-tb-haemoptysis-when-there-is-more-than-meets-the-eye
#11
Ubaid Feroze Seedat, Faheem Seedat
Haemoptysis is concerning for both patient and healthcare provider and points to the presence of severe underlying lung disease warranting investigation. Approximately 8% of patients with pulmonary tuberculosis (PTB) infection will experience haemoptysis at some point during their life [1;2] . The aetiology of haemoptysis in the setting of PTB is diverse and may occur during active or following prior PTB infection due to pulmonary complications. We describe the case of a 33-year-old female who presented with massive haemoptysis on two separate occasions within a five-month period...
2018: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/30094132/bilateral-candida-keratitis-in-an-hiv-patient-with-asymptomatic-genitourinary-candidiasis-in-uganda
#12
Simon Arunga, Teddy Kwaga, Astrid Leck, Victor H Hu, Matthew J Burton
A 35-year-old male presented with Candida keratitis in the left eye. He was HIV positive with a CD4 of 352 cells/µL. The eye quickly deteriorated, despite intensive antifungal treatment and was eviscerated. Five months later, he re-presented with Candida keratitis in his right eye. A focal source of Candida infection was suspected and a urine culture identified Candida spp, despite being asymptomatic for genitourinary candidiasis. He was subsequently treated with good outcome (max. 75 words).
December 2018: Medical Mycology Case Reports
https://www.readbyqxmd.com/read/30092813/smith-lemli-opitz-syndrome-presenting-as-acute-adrenal-crisis-in-a-child-a-case-report
#13
Chamara Jayamanne, Sajith Sandamal, Kasun Jayasundara, Mayoorathy Saranavabavananthan, Sachith Mettananda
BACKGROUND: Smith-Lemli-Opitz syndrome is a rare autosomal recessive disorder of cholesterol biosynthesis which is characterized by multiple congenital malformations and global developmental delay. Here we report the case of a 3-year-old, previously undiagnosed, child with Smith-Lemli-Opitz syndrome presenting with acute adrenal crisis, which is an extremely rare and atypical presentation of this disease. CASE PRESENTATION: A 3-year-old Sri Lankan Sinhalese boy without evidence of infection presented with circulatory collapse...
August 10, 2018: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/30091534/the-use-of-direct-immunofluorescence-and-nested-polymerase-chain-reaction-in-diagnosing-perinatal-infections-of-chlamydia-trachomatis
#14
Magdalena Frej-Mądrzak, Dorota Teryks-Wołyniec, Piotr Jankowski, Paulina Krochmal, Jolanta Sarowska, Agnieszka Jama-Kmiecik, Irena Choroszy-Król
BACKGROUND: Chlamydia infection is the most frequently reported infectious, sexually transmitted disease (STD). Generally, Chlamydia trachomatis (C. trachomatis) infection of neonates is the result of perinatal exposure to the mother's infected cervix. OBJECTIVES: The aim of the study was to estimate the frequency of infection caused by C. trachomatis in newborn infants. In this study of C. trachomatis perinatal infection, 107 infants born at the Wroclaw Medical University Clinic of Gynecology and Obstetrics (Poland) were tested to investigate whether C...
August 9, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/30083656/an-eye-for-azithromycin-review-of-the-literature
#15
REVIEW
Kostas A Kagkelaris, Olga E Makri, Constantine D Georgakopoulos, George D Panayiotakopoulos
Azithromycin is used widely in clinical practice and recently it is available in topical solution for ophthalmic use. The purpose of the current publication is to summarize the newest information on azithromycin's clinical usefulness over ocular diseases. A PubMed (National Library of Medicine) and a ScienceDirect search was conducted using the key phrases 'azithromycin', 'meibomian', 'blepharitis', 'trachoma', 'toxoplasmosis' from 2010 to 2017. Articles were limited to articles published in English or at least having an English abstract...
January 2018: Therapeutic advances in ophthalmology
https://www.readbyqxmd.com/read/30081842/periorbital-infections-and-conjunctivitis-due-to-panton-valentine-leukocidin-pvl-positive-staphylococcus-aureus-in-children
#16
Pia-Alice Hoppe, Leif G Hanitsch, Rasmus Leistner, Michaela Niebank, Christoph Bührer, Horst von Bernuth, Renate Krüger
BACKGROUND: Colonisation with Panton-Valentine Leukocidin expressing strains of Staphylococcus aureus (PVL + SA) is characterised by recurrent skin and soft tissue infections. While periorbital and orbital infections are common in children and frequently caused by S. aureus the role of PVL + SA in recurrent eye infections has not been studied. This study aimed to detect and report frequency and recurrence of periorbital or orbital infections as additional symptoms of PVL + SA colonisation in children...
August 6, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/30080662/occurrence-and-molecular-composition-of-methicillin-resistant-staphylococcus-aureus-isolated-from-ocular-surfaces-of-horses-presented-with-ophthalmologic-disease
#17
Tanawan Soimala, Antina Lübke-Becker, Stefan Schwarz, Andrea T Feßler, Charlotte Huber, Torsten Semmler, Roswitha Merle, Heidrun Gehlen, Johanna C Eule, Birgit Walther
Severe infections due to methicillin-resistant Staphylococcus aureus (MRSA) have been increasingly recognized in virtually all fields of veterinary medicine. Our objective was to study the occurrence, phylogenetic relationships and antimicrobial resistance properties of MRSA isolated from ocular surfaces of horses prior to invasive procedures. Within a 49-week sampling period, ocular swabs obtained from 46 eyes of 44 horses, including eyes with clinical signs of conjunctivitis/blepharitis, keratitis or uveitis were screened for the presence of S...
August 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/30075555/retinitis-associated-with-double-infection-of-epstein-barr-virus-and-varicella-zoster-virus-a-case-report
#18
Tomohito Sato, Riki Kitamura, Toshikatsu Kaburaki, Masaru Takeuchi
RATIONALE: Chronic uveitis with immunosuppressive agents could develop chronic herpetic retinitis with varicella-zoster virus (VZV) or herpes simplex virus (HSV). Ocular Epstein-Barr virus (EBV) infection develops uveitis and vitritis, but the clinical feature of EBV retinitis is not typical as a viral retinitis. EBV retinitis is rare, and only a few cases of EBV retinitis have been reported. Herein, we describe a case of retinitis with EBV and VZV which were the primary viruses verified by multiplex polymerase chain reaction (PCR)...
August 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/30073760/outcomes-of-combined-keratoplasty-and-pars-plana-vitrectomy-for-endophthalmitis-with-compromised-corneal-clarity
#19
Abhishek Dave, Manisha Acharaya, Manisha Agarwal, Prachi A Dave, Manisha Singh, Umang Mathur
IMPORTANCE: Studies evaluating combined penetrating keratoplasty and pars plana vitrectomy for infectious etiologies are limited. BACKGROUND: To evaluate the outcomes of combined penetrating keratoplasty and pars plana vitrectomy in patients with endophthalmitis and poor corneal clarity. DESIGN: Retrospective interventional case series conducted at a tertiary care eye hospital in North India. PARTICIPANTS: Review of records of 43 eyes of 43 patients...
August 2, 2018: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/30073142/avian-trichomonosis-mortality-events-in-band-tailed-pigeons-patagioenas-fasciata-in-california-during-winter-2014-2015
#20
Krysta H Rogers, Yvette A Girard, Leslie W Woods, Christine K Johnson
Avian trichomonosis is an upper digestive tract disease of birds typically caused by the protozoan parasite Trichomonas gallinae . In California (U.S.A), trichomonosis is known to cause periodic epidemics in the Pacific Coast band-tailed pigeon ( Patagioenas fasciata monolis ), a migratory upland game bird. We summarize the mortality events that occurred during winter 2014-2015 including the duration, estimated mortality, pathology, and genetic identity of infecting parasites. Increased mortality was reported from locations in 25 counties between November 2014 and June 2015...
December 2018: International Journal for Parasitology. Parasites and Wildlife
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