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Vascular endothelial growth factors

Priya K Prasai, Bandana Shrestha, A Wayne Orr, Christopher B Pattillo
The angiogenic capacity of local tissue critically regulates the response to ischemic injury. Elevated reactive oxygen species production, commonly associated with ischemic injury, has been shown to promote phosphorylation of the vascular endothelial growth factor receptor 2 (VEGFR2), a critical regulator of angiogenesis. Previous data from our lab demonstrated that diminished levels of the antioxidant glutathione positively augment ischemic angiogenesis. Here, we sought to determine the relationship between glutathione levels and oxidative stress in VEGFR2 signaling...
July 25, 2018: Redox Biology
Yasmina M Abd-Elhakim, Mohamed M Hashem, Abeer E El-Metwally, Abeer Anwar, Khaled Abo-El-Sooud, Gihan G Moustafa, Haytham A Ali
The haemato-immunotoxic effects of the food colourants tartrazine and chlorophyll were evaluated. Thirty adult Sprague Dawley rats were distributed into three groups and orally administered water, tartrazine (1.35 mg/kg), or chlorophyll (1.35 mg/kg) daily for 90 days. Erythrogram and leukogram profiles were evaluated. The lysozyme, nitric oxide, phagocytic activity, and immunoglobulin levels were measured. Histological and immunohistochemical evaluations of splenic tissues were conducted. Changes in the interleukin (IL) 1β, 6, and 10 mRNA expression levels were assessed...
August 6, 2018: International Immunopharmacology
Vida Naderi-Boldaji, Siyavash Joukar, Ali Noorafshan, Alireza Raji-Amirhasani, Samaneh Naderi-Boldaji, Mohammad-Abbas Bejeshk
AIMS: Low-intensity aerobic training along with limbs blood flow restriction can improve mass and strength of skeletal muscle, but its effects on aging heart structure and performance is unidentified. We investigated the effects of this model of training on myocardial function, histology and angiogenesis in old male rats. MAIN METHODS: Animals randomly were divided into control (Ctl), sham-operated (Sh), limbs blood flow restriction (BFR), sham-operated plus 10 weeks low intensity treadmill exercise (Sh + Ex), and BFR plus exercise (BFR + Ex) groups...
August 7, 2018: Life Sciences
Jennifer K Sun, Adam R Glassman, Wesley T Beaulieu, Cynthia R Stockdale, Neil M Bressler, Christina Flaxel, Jeffrey G Gross, Michel Shami, Lee M Jampol
OBJECTIVE: Present rationale, guidelines, and results of ranibizumab treatment for proliferative diabetic retinopathy (PDR) in Diabetic Retinopathy Clinical Research Network ( Protocol S. DESIGN: Post hoc analyses from a randomized clinical trial. PARTICIPANTS: Three hundred and five participants with 394 study eyes having PDR without prior PRP. INTERVENTION: Post hoc analyses from a randomized clinical trial of 0...
August 7, 2018: Ophthalmology
Yong-Chao Jiang, Xiao-Feng Wang, Yi-Yang Xu, Yu-Hui Qiao, Xin Guo, Dong-Fang Wang, Qian Li, Lih-Sheng Turng
During the regeneration of tissues and organs, growth factors (GFs) play a vital role by affecting cell behavior. However, because of the low half-life time and quick degradation of GFs, their stimulations on cells are relatively short and discontinuous. In this study, a releasing scaffold platform, consisting of polycaprolactone (PCL) nanofibers and vascular endothelial growth factor (VEGF)-encapsulated gelatin particles, was developed to extend the influence of GFs on mesenchymal stem cells (MSCs) and endothelial cells (ECs)...
August 10, 2018: Biomacromolecules
Bing-Qin Guo, Wen-Qiao Lu
Background & aim: At present, numerous reports have shown that high/positive expression of tissue vascular endothelial growth factor (VEGF) may be associated with the prognosis of patients with ovarian cancer. However, their results still remained controversy. Thus, this meta-analysis was designed to analyze and assess the prognostic value of tissue VEGF expression in patients with ovarian cancer. Method: We searched PubMed, Embase, Cochrane Library and Web of Science to October, 2017...
July 17, 2018: Oncotarget
Yona Nicolau, Fayez Bany-Mohammed, Charles L Cai, Jacob V Aranda, Kay D Beharry
Vascular endothelial growth factor (VEGF) is a potent mitogen that regulates proliferation, migration, and tube formation of endothelial cells (EC). VEGF has recently become a target for severe retinopathy of prematurity (ROP) therapy. We tested the hypothesis that a specific VEGF isoform and/or receptor acts synergistically with insulin-like growth factor (IGF)-I to alter normal retinal microvascular EC angiogenesis and RNA interference can be used to reverse VEGF effects. We used small interfering RNA (SiRNA) transfection to target VEGF isoforms, IGFs, and their receptors in human retinal microvascular endothelial cells (HRECs)...
2018: American Journal of Translational Research
Jingrui Chen, Jing Wei, Yuting Huang, Yuling Ma, Jingyu Ni, Min Li, Yan Zhu, Xiumei Gao, Guanwei Fan
Stem cell-based therapies have the potential to dramatically transform the treatment and prognosis of myocardial infarction (MI), and mesenchymal stem cells (MSCs) have been suggested as a promising cell population to ameliorate the heart remodeling in post-MI. However, poor implantation and survival in ischemic myocardium restrict its efficacy and application. In this study, we sought to use the unique mode of action of Chinese medicine to improve this situation. Surrounding the myocardial infarct area, we performed a multi-point MSC transplantation and administered in conjunction with Danhong injection, which is mainly used for the treatment of MI...
2018: Frontiers in Physiology
Feng Zhang, Georgia Zarkada, Jinah Han, Jinyu Li, Alexandre Dubrac, Roxana Ola, Gael Genet, Kevin Boyé, Pauline Michon, Steffen E Künzel, Joao Paulo Camporez, Abhishek K Singh, Guo-Hua Fong, Michael Simons, Patrick Tso, Carlos Fernández-Hernando, Gerald I Shulman, William C Sessa, Anne Eichmann
Excess dietary lipid uptake causes obesity, a major global health problem. Enterocyte-absorbed lipids are packaged into chylomicrons, which enter the bloodstream through intestinal lymphatic vessels called lacteals. Here, we show that preventing lacteal chylomicron uptake by inducible endothelial genetic deletion of Neuropilin1 ( Nrp1 ) and Vascular endothelial growth factor receptor 1 ( Vegfr1 ; also known as Flt1 ) renders mice resistant to diet-induced obesity. Absence of NRP1 and FLT1 receptors increased VEGF-A bioavailability and signaling through VEGFR2, inducing lacteal junction zippering and chylomicron malabsorption...
August 10, 2018: Science
Matthew D Young, Thomas J Mitchell, Felipe A Vieira Braga, Maxine G B Tran, Benjamin J Stewart, John R Ferdinand, Grace Collord, Rachel A Botting, Dorin-Mirel Popescu, Kevin W Loudon, Roser Vento-Tormo, Emily Stephenson, Alex Cagan, Sarah J Farndon, Martin Del Castillo Velasco-Herrera, Charlotte Guzzo, Nathan Richoz, Lira Mamanova, Tevita Aho, James N Armitage, Antony C P Riddick, Imran Mushtaq, Stephen Farrell, Dyanne Rampling, James Nicholson, Andrew Filby, Johanna Burge, Steven Lisgo, Patrick H Maxwell, Susan Lindsay, Anne Y Warren, Grant D Stewart, Neil Sebire, Nicholas Coleman, Muzlifah Haniffa, Sarah A Teichmann, Menna Clatworthy, Sam Behjati
Messenger RNA encodes cellular function and phenotype. In the context of human cancer, it defines the identities of malignant cells and the diversity of tumor tissue. We studied 72,501 single-cell transcriptomes of human renal tumors and normal tissue from fetal, pediatric, and adult kidneys. We matched childhood Wilms tumor with specific fetal cell types, thus providing evidence for the hypothesis that Wilms tumor cells are aberrant fetal cells. In adult renal cell carcinoma, we identified a canonical cancer transcriptome that matched a little-known subtype of proximal convoluted tubular cell...
August 10, 2018: Science
Nafiye Yılmaz, Banu Seven, Hakan Timur, Ayçağ Yorgancı, Hasan Ali İnal, Müberra Namlı Kalem, Ziya Kalem, Özge Han, Banu Bilezikçi
BACKGROUND: Ginger (Zingiber officinale) is a well known and extensively used antioxidant in traditional remedies. In this study, we aimed to investigate the effects of ginger powder on ovarian folliculogenesis and implantation in rats. METHODS: There were two study groups. In the 5-day treatment group (one estrous cycle), 100 mg ginger powder, 200 mg ginger powder or distilled water was given for 5 days to the three subgroups each containing seven rats. In the 10-day treatment group, same doses were given for 10 days (two estrous cycle) to the three subgroups each containing seven rats...
August 7, 2018: Journal of the Chinese Medical Association: JCMA
Jordi Pedragosa, Angélica Salas-Perdomo, Mattia Gallizioli, Roger Cugota, Francesc Miró-Mur, Ferran Briansó, Carles Justicia, Fernando Pérez-Asensio, Leonardo Marquez-Kisinousky, Xabier Urra, Anna Gieryng, Bozena Kaminska, Angel Chamorro, Anna M Planas
The central nervous system (CNS) contains several types of immune cells located in specific anatomic compartments. Macrophages reside at the CNS borders surrounding the brain vessels, in leptomeningeal spaces and the choroid plexus, where they interact with the vasculature and play immunological surveillance and scavenging functions. We investigated the phenotypic changes and role of these macrophages in response to acute ischemic stroke. Given that CD163 expression is a hallmark of perivascular and meningeal macrophages in the rat and human brain, we isolated CD163+ brain macrophages by fluorescence activated cell sorting...
August 9, 2018: Acta Neuropathologica Communications
Kei Miwata, Hirokazu Okamoto, Taku Nakashima, Daisuke Ihara, Yasushi Horimasu, Takeshi Masuda, Shintaro Miyamoto, Hiroshi Iwamoto, Kazunori Fujitaka, Hironobu Hamada, Ayumi Shibata, Takaaki Ito, Tomoyuki Okuda, Noboru Hattori
Inhalation therapy using small-interfering RNA (siRNA) is a potentially effective therapeutic strategy for lung cancer because of its high gene-silencing effects and sequence specificity. Previous studies reported that intratracheal administration of siRNA using pressurized metered dose inhalers or nebulizers could suppress tumor growth in murine lung metastatic models. Although dry powder inhalers are promising devices due to their low cost, good portability, and preservability, the anti-tumor effects of siRNA dry powder have not been elucidated...
July 17, 2018: Molecular Therapy. Nucleic Acids
Jia-Peng Wu, Xue-Zhi Li, Ying Wang, Lin Ma, Tai-Wan Yao, Yong-Yue Zhang, Fei Long
OBJECTIVE: To observe the effect of electroacupuncture (EA) and EA combined with intracerebral injection of vascular endothelial growth factor (VEGF) on endoplasmic reticulum stress (ERS) related proteins and genes as activating transcription factor (ATF 6), inositol requiring enzyme-1 (IRE 1), CCAAT/enhancer binding protein homologous protein (CHOP), X box-binding protein-1 (XBP 1) of cerebral ischemia reperfusion injury (CIRI) rats, so as to study its repair effect for CIRI. METHODS: Forty male SD rats were equally and randomly divided into 5 groups: sham operation, model, EA, VEGF and EA+VEGF groups ( n =8)...
June 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
Sang-Pil Choi, Yong-Chan Choi, Jun Yang, Chang-Yong Choi, Chang-Hee Lee, Seok-Jin Kang, In-Byung Park, Taehoon Chun
The reproductive success of mammals is largely dependent on the interaction between maternal and foetal interfaces during early pregnancy. Particularly, immune cells which reside at the maternal endometrium can modulate the conception and placental vascularization. In this study, we analysed the transcription of genes involved in early pregnancy from endometrium and peripheral blood mononuclear cells (PBMCs) of pregnant pigs with different parity. Briefly, three groups of female pigs were divided based on parity (0, 2 and 5) and each group was artificially inseminated...
August 9, 2018: Reproduction in Domestic Animals, Zuchthygiene
Victoria A Flower, Shaney L Barratt, Stephen Ward, John D Pauling
The pathological hallmarks of Systemic sclerosis (SSc) constitute an inter-related triad of autoimmunity, vasculopathy and tissue remodeling. Many signaling mediators have been implicated in SSc pathology; most focusing on individual components of this pathogenic triad and current treatment paradigms tend to approach management of such as distinct entities. The present review shall examine the role of vascular endothelial growth factor (VEGF) in SSc pathogenesis. We shall outline potential mechanisms whereby differential vascular endothelial growth factor-A (VEGF-A) isoform expression (through conventional and alternative VEGF-A splicing,) may influence the relevant burden of vasculopathy and fibrosis offering novel insight into clinical heterogeneity and disease progression in SSc...
August 9, 2018: Current Rheumatology Reviews
Francesco Moccia, Roberto Berra-Romani, Vittorio Rosti
Endothelial progenitor cells (EPCs) are mobilized in peripheral blood to rescue blood perfusion in ischemic tissues. Several approaches were, therefore, designed to inject autologous EPCs and induce therapeutic angiogenesis in patients affected by cardiovascular disorders. Endothelial colony forming cells (ECFCs) represent the only truly endothelial precursor and are regarded as the most suitable substrate for cell based therapy of ischemic diseases. Intracellular Ca2+ signalling drives ECFC proliferation, migration, homing and neovessel formation...
August 8, 2018: Current Pharmaceutical Biotechnology
Jae Byoung Chae, Chang Rae Rho, Jeong Ah Shin, Jungmook Lyu, Seungbum Kang
PURPOSE: Anti-vascular endothelial growth factor (VEGF) agents have been used for the last 10 years, but their safety profile, including cytotoxicity against various ocular cells such as retinal pigment epithelial (RPE) cells, remains a serious concern. Safety studies of VEGF agents conducted to date have primarily relied on healthy RPE cells. In this study, we assessed the safety of three anti-VEGF agents, namely, ranibizumab, bevacizumab, and aflibercept, on senescent RPE cells. METHODS: Senescent human induced pluripotent stem cell-derived RPE cells were generated by continuous replication and confirmed with senescence biomarkers...
August 2018: Korean Journal of Ophthalmology: KJO
Margrit Hollborn, Charlotte Ackmann, Heidrun Kuhrt, Fabian Doktor, Leon Kohen, Peter Wiedemann, Andreas Bringmann
Purpose: Variants of complement factor genes, hypoxia and oxidative stress of the outer retina, and systemic hypertension affect the risk of age-related macular degeneration. Hypertension often results from the high intake of dietary salt that increases extracellular osmolarity. We determined the effects of extracellular hyperosmolarity, hypoxia, and oxidative stress on the expression of complement genes in cultured (dedifferentiated) human RPE cells and investigated the effects of C9 siRNA and C9 protein on RPE cells...
2018: Molecular Vision
Natalia Ziemkiewicz, Muhamed Talovic, Josh Madsen, Lindsay Hill, Robert Scheidt, Anjali Patel, Gabriel Haas, Madison Marcinczyk, Silviya Zustiak, Koyal Garg
Skeletal muscle has a remarkable regenerative capability following mild physical or chemical insult. However, following a critical loss of muscle tissue, the regeneration process is impaired due to the inadequate myogenic activity of muscle resident stem cells (i.e., satellite cells). Laminin (LM) is a heterotrimeric structural protein in the satellite cell niche that is crucial for maintaining its function. In this study, we created hydrogels composed of poly (ethylene glycol) (PEG) and LM-111 to provide an elastic substrate for satellite cell proliferation at the site of injury...
August 9, 2018: Biomedical Materials
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