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molecularly imprinted

Wan Qiu Xia, Hui Cai Zhang, Geng Nan Wang, Jing Liu, Jian Ping Wang
The residues of phenothiazines and benzodiazepines in foods of animal origin are dangerous to consumers. For inspection of their abuses, this study for the first time reported on the use of a chemiluminescence array sensor for the simultaneous determination of four phenothiazines and five benzodiazepines in pig urine. Two molecularly imprinted polymers were coated in different wells of a conventional 96-well microtiter plate as the recognition reagents. After sample loading, the absorbed analytes were initiated directly by using an imidazole enhanced bis(2,4,6-trichlorophenyl)oxalate-hydrogen peroxide system to emit light...
December 12, 2018: Luminescence: the Journal of Biological and Chemical Luminescence
Zhong Zhang, Xin Ma, Mengfan Jia, Bowei Li, Jianhui Rong, Xingbin Yang
Rapid detection of pesticides in fruits is an ongoing challenge. The objective of the present study was to develop novel fluorescent microfluidic paper chips for specific recognition and sensitive detection of the pesticide 2,4-D through the electron-transfer-induced fluorescence quenching mechanism. CdTe quantum dots (QDs) were deposited onto cellulose paper (base material) to yield imprinted paper chips (paper@QDs@MIPs). This method allows the transferability of the molecularly imprinted fluorescence sensor from the liquid phase to the solid phase (paper base) for rapid and portable analysis...
December 14, 2018: Analyst
Wenhui Lu, Jie Liu, Jinhua Li, Xiaoyan Wang, Min Lv, Rong Cui, Lingxin Chen
Novel dual-template molecularly imprinted polymers (dt-MIPs) were prepared by simple and facile precipitation polymerization using norfloxacin (NOR) and enrofloxacin (ENR) as templates for simultaneous selective recognition and extraction of the two fluoroquinolones (FQs). The as-prepared dt-MIPs exhibited high adsorption capacity and excellent selectivity towards NOR and ENR. Several main parameters affecting the efficiency of dt-MIP based dispersive solid-phase extraction (DSPE) were systematically investigated, coupled with high performance liquid chromatography determination...
December 14, 2018: Analyst
Si Min Lai, Jianpeng Sheng, Pravesh Gupta, Laurent Renia, Kaibo Duan, Francesca Zolezzi, Klaus Karjalainen, Evan W Newell, Christiane Ruedl
Inflammation-induced disappearance of tissue-resident macrophages represents a key pathogen defense mechanism. Using a model of systemic blood-stage malaria, we studied the dynamics of tissue-resident macrophages in multiple organs to determine how they are depleted and refilled during the course of disease. We show that Plasmodium infection results in a transient loss of embryonically established resident macrophages prior to the parasitemia peak. Fate-mapping analysis reveals that inflammatory monocytes contribute to the repopulation of the emptied niches of splenic red pulp macrophages and hepatic Kupffer cells, while lung alveolar macrophages refill their niche predominantly through self-renewal...
December 11, 2018: Cell Reports
Guoliang Lyu, Le Zong, Chao Zhang, Xiaoke Huang, Wenbing Xie, Junnan Fang, Yiting Guan, Lijun Zhang, Ting Ni, Jun Gu, Wei Tao
Stimulatory regulators for DNA methyltransferase activity, such as Dnmt3L and some Dnmt3b isoforms, affect DNA methylation patterns, thereby maintaining gene body methylation and maternal methylation imprinting, as well as the methylation landscape of pluripotent cells. Here we show that metastasis-related methyltransferase 1 (Merm1), a protein deleted in individuals with Williams-Beuren syndrome, acts as a repressive regulator of Dnmt3a. Merm1 interacts with Dnmt3a and represses its methyltransferase activity with the requirement of the binding motif for S-adenosyl-L-methionine...
March 21, 2018: Journal of Molecular Cell Biology
Anushi Shukla, Neha Bunkar, Rajat Kumar, Arpit Bhargava, Rajnarayan Tiwari, Koel Chaudhury, Irina Y Goryacheva, Pradyumna K Mishra
Air pollution is one of the leading causes of deaths in Southeast Asian countries including India. Exposure to air pollutants affects vital cellular mechanisms and is intimately linked with the etiology of a number of chronic diseases. Earlier work from our laboratory has shown that airborne particulate matter disturbs the mitochondrial machinery and causes significant damage to the epigenome. Mitochondrial reactive oxygen species possess the ability to trigger redox-sensitive signaling mechanisms and induce irreversible epigenomic changes...
November 28, 2018: Science of the Total Environment
Jia-Wei Zhang, Ling Tan, Yu-Zhen Zhang, Guo-Can Zheng, Zhi-Ning Xia, Chong-Zhi Wang, Lian-Di Zhou, Qi-Hui Zhang, Chun-Su Yuan
In this work, surface molecularly imprinted polymers (SMIPs) were prepared as a specific sorbent to remove the limonin from the lemon juice for the first time, and then the MIPs containing limonin were directly made into a water-soluble gel to treat inflammation of mice. The resulting polymers were characterized by scanning electron microscopy, transmission electron microscopy and Fourier transform infrared spectrometer spectra. And the polymerization conditions and adsorption performances of the resultant nanomaterials were further investigated in detail...
November 22, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Huaqing Yan, Jiangfeng Li, Yufan Ying, Haiyun Xie, Hong Chen, Xin Xu, Xiangyi Zheng
Emerging research has suggested that miRNAs play a significant role in oncogenesis and tumor progression by regulating multiple molecular pathways. Here, we investigated miR-300, which inhibited bladder cancer (BCa) migration by regulating the SP1/MMP9 pathway. miR-300, belonging to the DLK1-DIO3 miRNA cluster, is frequently expressed at lower levels in BCa tissue than in adjacent normal tissue due to DNA methylation. Reinforced expression of miR-300 significantly suppressed the migration of BCa cells. We carried out a search of online databases to predict potential targets of miR-300...
December 9, 2018: Cell Cycle
Lianghui Luo, Wenbao Feng, Wanting Hu, Chuanyan Chen, Hang Gong, Changqun Cai
A novel designed fluorescence molecularly imprinted polymer (MIP) probe made from CdTe/CdS quantum dot (QD)-based silica nanoparticles (SiO2@MPS-CdTe/CdS) was successfully created via a sol-gel process. The target virus - hepatitis A virus (HAV) was selectively captured by imprinted polymer layer, resulting in the fluorescence quenching of the QDs within 20 min, which could be explained by the energy transfer mechanism. Under optimized conditions, the limit of detection for the SiO2@MPS-CdTe/CdS MIP was as low as 88 pmol∙L-1, and excellent linearity was obtained from 0...
November 14, 2018: Methods and Applications in Fluorescence
Xiaoli Sun, Muhua Wang, Luoxing Yang, Huiping Wen, Lianggui Wang, Ting Li, Chunlan Tang, Jiajia Yang
Dummy molecularly imprinted polymer (DMIP) for imidazole fungicides was prepared for the first time using alpha-(2,4-Dichlorophenyl)-1H-imidazole-1-ethanol (DCE) as the fragment template. The imprinting selectivity of DCE-DMIP was evaluated for climbazole (CBZ), clotrimazole (CMZ) and miconazole (MNZ) by liquid chromatography, imprinting factors of 10.9, 10.8 and >10.7 were achieved, respectively. Heterogeneous binding sites were found in the DCE-DMIP, the corresponding saturation capacity and dissociation constant for the high affinity binding sites were 13...
November 29, 2018: Journal of Chromatography. A
Kisho Mori, Mitsuhiro Hirase, Takahiro Morishige, Eri Takano, Hirobumi Sunayama, Yukiya Kitayama, Sachiko Inubushi, Ryohei Sasaki, Masakazu Yashiro, Toshifumi Takeuchi
Exosomes are small (30-100 nm) membrane vesicles serving as regulatory agents for intercellular communication in cancers. Currently, exosomes are detected by immuno-based assays with appropriate pretreatments like ultracentrifugation and are time consuming (>12 h). We present a novel pretreatment-free fluorescence-sensing platform for intact exosomes, wherein exchangeable antibodies and fluorescent reporter molecules were aligned inside exosome-binding cavities. Such antibody-containing fluorescent reporter-grafted nanocavities were prepared on a substrate by well-designed molecular imprinting and post-imprinting modifications to introduce antibodies and fluorescent reporter molecules only inside the binding nanocavities, enabling sufficiently high sensitivity to detect intact exosomes without pretreatment...
December 4, 2018: Angewandte Chemie
Mark A Pianka, Alec T McIntosh, Sahaj D Patel, Pegah R Bakhshi, Mira Jung
Genetic imprinting is the process of epigenetic labelling or silencing of particular genes, based on the maternal or paternal origin of the gene, in a heritable pattern. The incidence of imprinting disorders has become a growing concern due to the potential association between these congenital syndromes and assisted reproductive technologies (ARTs). This review presents a general summary of the imprinting process as well as the current knowledge surrounding the genetic and epigenetic underpinnings of the most prevalent imprinting disorders: Beckwith-Wiedemann syndrome (BWS), Silver-Russell syndrome (SRS), Prader-Willi syndrome (PWS), and Angelman syndrome (AS)...
2018: American Journal of Stem Cells
L C Houtepen, R Hardy, J Maddock, D Kuh, E L Anderson, C L Relton, M J Suderman, L D Howe
Childhood adversity affects later health, but the underlying molecular mechanisms are unclear. Although there is some evidence from animal models and case-control studies of a role for DNA methylation, evidence from human population-based studies is limited. In two cohorts (mothers from the Avon Longitudinal Study of Parents and Children, ALSPAC, n = 780 and women from the MRC National Survey of Health and Development, NSHD, n = 552), we assessed the association of seven adverse childhood experiences (ACEs: parental physical illness, parental mental illness, parental death, parental separation, suboptimal maternal bonding, childhood illness and child maltreatment) as well as their combination (ACE score) with genome-wide DNA methylation levels measured using the Illumina Infinium HumanMethylation450 BeadChip in peripheral blood at mean age 47 years (ALSPAC) and in buccal cells at age 53 years (NSHD)...
December 3, 2018: Translational Psychiatry
Hidenori Hidaka, Ken Higashimoto, Saori Aoki, Hiroyuki Mishima, Chisa Hayashida, Toshiyuki Maeda, Yasuo Koga, Hitomi Yatsuki, Keiichiro Joh, Hirokazu Noshiro, Ryuichi Iwakiri, Atsushi Kawaguchi, Koh-Ichiro Yoshiura, Kazuma Fujimoto, Hidenobu Soejima
BACKGROUND: Imprinted genes are regulated by DNA methylation at imprinting-associated differentially methylated regions (iDMRs). Abnormal expression of imprinted genes is implicated in imprinting disorders and tumors. In colorectal cancer (CRC), methylation and imprinting status of the IGF2/H19 domain have been studied. However, no comprehensive methylation analysis of iDMRs in CRC has been reported. Furthermore, the relationship between iDMR methylation status and other methylation-related issues, such as CpG island methylator phenotype (CIMP) and long interspersed element-1 (LINE-1) methylation, remains unclear...
December 4, 2018: Clinical Epigenetics
Sayyed Hossein Hashemi, Massoud Kaykhaii, Ahmad Jamali Keikha, Zahra Sajjadi
In this paper, a rapid, selective and effective technique, pipette-tip solid phase extraction based on molecularly imprinted polymer, was used for extraction and pre-concentration of methyl red (MR) prior to its determination by spectrophotometer. Variables influencing extraction efficiency including type and volume of eluent solvent, sample volume, number of cycles of extraction and elution, amount of sorbent and pH of the sample solution were optimized with two methods of one-variable-at-a-time and response surface methodology (RSM)...
December 2018: Marine Pollution Bulletin
Özgecan Erdem, Yeşeren Saylan, Nilüfer Cihangir, Adil Denizli
Human fecal contamination poses a crucial environmental and health threat in recent years, resulting in the difficulties of access to clean water. According to the World Health Organization, several fecal bacteria, particularly Enterococci species, are present in human intestinal flora. Enterococcus faecalis (E. faecalis) is one of the indicator bacteria that have been utilized as a pollution indicator in water. However, existing technologies and detection strategies face multiple challenges in terms of low affinity for detection and labelling requirements that limit their access to large scale applications...
November 19, 2018: Biosensors & Bioelectronics
Dianwei Zhang, Huilin Liu, Weitao Geng, Yanping Wang
Molecular imprinting is a technique for polymerization using a template molecule to produce cavities in the polymer with high selectivity. The successful synthesis of a dual-function molecularly-imprinted optopolymer (MIOP) based on quantum dots-grafted covalent-organic frameworks as adsorbents has allowed the simultaneous detection of tyramine by optosensing and solid-phase extraction coupled with high performance liquid chromatography (SPE-HPLC). The MIOP, obtained by a one-pot reverse microemulsion polymerization, selectively measured tyramine from fermented meat products...
March 30, 2019: Food Chemistry
Xiao-Yu Sun, Run-Tian Ma, Juan Chen, Yan-Ping Shi
Boronate-affinity based molecularly imprinted polymers (MIPs) are beset by the unsatisfied adsorption capacity and narrow working pH ranges. A magnetic molecularly imprinted polymer containing phenylboronic acid groups was placed on the surface of Fe3 O4 (magnetite) microspheres coated with porous TiO2 (Fe3 O4 @pTiO2 @MIP). In contrast to its silica analog (Fe3 O4 @SiO2 @MIP), the flowerlike Fe3 O4 @pTiO2 offers more binding sites for templates. Thus, the adsorption capacity of the Fe3 O4 @pTiO2 @MIP is strongly enhanced...
November 29, 2018: Mikrochimica Acta
Guichun Mo, Xinxin He, Chunqin Zhou, Dongmei Ya, Jinsu Feng, Chunhe Yu, Biyang Deng
In this work, a boronate-affinity sandwich electrochemiluminescence (ECL) sensor was constructed to detect alpha-fetoprotein (AFP) based on a multiple signal amplification strategy. Gold nanoparticles (AuNPs) were utilized and modified on the surface with chitosan in order to facilitate electron transfer. The composite of the molecularly imprinted polymer (MIP) enhanced the selectivity of alpha-fetoprotein detection. 4-mercaptophenylboronic acid (MPBA) was used as the tracing tag for capture of alpha-fetoprotein...
November 20, 2018: Biosensors & Bioelectronics
Guifen Zhu, Guohao Cheng, Li Wang, Wenna Yu, Peiyun Wang, Jing Fan
Toward improving the selective adsorption performance of molecularly imprinted polymers in strong polar solvents, in this work, a new ionic liquid functional monomer, 1-butyl-3-vinylimidazolium bromide, was used to synthesize sulfamethoxazole imprinted polymer in methanol. The resulting molecularly imprinted polymer was characterized by Fourier transform infrared spectra and scanning electron microscopy, and the rebinding mechanism of the molecularly imprinted polymer for sulfonamides was studied. A static equilibrium experiment revealed that the as-obtained molecularly imprinted polymer had higher molecular recognition for sulfonamides (e...
November 28, 2018: Journal of Separation Science
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