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Stroma mesenchymal

Diego Alberti, Cristina Grange, Stefano Porta, Silvio Aime, Lorenzo Tei, Simonetta Geninatti Crich
Recent research results report that extracellular vesicles (EVs) have a central role in both physiological and pathological processes involving intercellular communication. Herein, a simple EVs labeling procedure based on the metabolic labeling of secreting cells (mesenchymal stroma cells, MSCs) with a fluorescein-containing bio-orthogonal dye is described. This procedure was carried out by incubating cells for 72 h with tetraacetylated N -azidoacetyl-d-mannosamine (Ac4 ManNAz), a modified sugar containing an azido group that, upon incorporation on the external surface of the cytoplasmatic cell membrane, is specifically conjugated with cyclooctyne-modified fluorescein isothiocyanate (ADIBO-FITC)...
July 31, 2018: ACS Omega
Eugene J Koay, Yeonju Lee, Vittorio Cristini, John S Lowengrub, Ya'an Kang, F Anthony San Lucas, Brian P Hobbs, Rong Ye, Dalia Elganainy, Muayad Almahariq, Ahmed M Amer, Shun Yu, Deyali Chatterjee, Huaming Yan, Peter C Park, Mayrim V Rios Perez, Dali Li, Naveen Garg, Kim Reiss, Anil Chauhan, Mohamed M Zaid, Newsha Nikzad, Robert A Wolff, Milind Javle, Gauri R Varadhachary, Rachna T Shroff, Prajnan Das, Jeffrey E Lee, Mauro Ferrari, Anirban Maitra, Cullen M Taniguchi, Michael P Kim, Christopher H Crane, Matthew H G Katz, Huamin Wang, Priya Bhosale, Eric P Tamm, Jason B Fleming
BACKGROUND AND AIMS: Pancreatic ductal adenocarcinoma (PDAC) is a heterogeneous disease with variable presentations and natural histories of disease. We hypothesized that different morphologic characteristics of PDAC tumors on diagnostic computed tomography (CT) scans would reflect their underlying biology. METHODS: We developed a quantitative method to categorize the PDAC morphology on pre-therapy CT scans from multiple datasets of patients with resectable and metastatic disease, and correlated these patterns with clinical/pathologic measurements...
August 6, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Belen Pan-Castillo, Salvatore A Gazze, Samantha Thomas, Christopher Lucas, Lavinia Margarit, Deyarina Gonzalez, Lewis W Francis, Robert Steven Conlan
During decidualization, human mesenchymal-like endometrial stromal cells undergo well characterized cellular and molecular transformations in preparation for accepting a developing embryo. Modulation of cellular biophysical properties during decidualization is likely to be important in receptivity and support of the embryo in the uterus. Here we assess the biophysical properties of human endometrial stromal cells including topography, roughness, adhesiveness and stiffness in cells undergoing in vitro decidualization...
July 20, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
Xingchen Pang, Yi Wang, Sijie Zhang, Zengqi Tan, Jia Guo, Feng Guan, Xiang Li
Mesenchymal stromal cells are an important component of the bone marrow microenvironment (niche), where they support hematopoiesis via direct cell‑cell interactions with hematopoietic stem and progenitor cells, and by releasing soluble factors. Glycans, including N‑glycans, are involved in numerous biological processes, including inflammation, cell‑cell interactions, as well as cancer development and progression. Lectin‑based microarray analysis has provided a powerful new tool in recent years, for the investigation of aberrantly expressed N‑glycans and their functions in the bone marrow microenvironment...
September 2018: Oncology Reports
Francesco De Francesco, Silvia Mannucci, Giamaica Conti, Elena Dai Prè, Andrea Sbarbati, Michele Riccio
Adipose tissue possesses phenotypic gene expression characteristics that are similar to human mesenchymal stem cells (hMSCs). Nevertheless, the multilineage potential may be inhibited, and cells may not expand adequately to satisfy the requirements of Good Manufacturing Practice (cGMP). An autologous hMSC-enriched fat product would fulfil the void from a biomedical and clinical perspective. In this study, we suggest a novel mechanism using a closed system without enzymes, additives or other modifications, which will produce non-expanded, accessible material...
July 15, 2018: International Journal of Molecular Sciences
Seon-Yeong Jeong, Jin-A Kim, Il-Hoan Oh
Accumulating studies have shown the cellular nature of hematopoietic stem cell (HSC) niche in bone marrow (BM) and their degenerative changes under leukemic conditions. However, the dynamic adaptation of niche cells to changes in physiological stimulatory signals remains largely uncharacterized. Here, we have established a niche stimulation model induced by 5-fluorouracil. This model reveals a rapid and reversible conversion of mesenchymal cells into niche-like stromal cells, which exhibit a platelet-derived growth factor receptor-alpha+ /leptin receptor+ (PL) phenotype...
July 13, 2018: Stem Cells
Tilman Ahlfeld, Falko Doberenz, David Kilian, Corina Vater, Paula Korn, Günter Lauer, Anja Lode, Michael Gelinsky
Due to their characteristic resemblance of the mineral component of bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on the development of pasty calcium phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion-based additive manufacturing, namely 3D plotting. While it could be demonstrated that sensitive substances like growth factors can be integrated in such printed CPC scaffolds without impairment of their biological activity live cells cannot be suspended in CPC as they may not be functional when enclosed in a solid and stiff matrix...
July 27, 2018: Biofabrication
Nicola Alessio, Antonietta Stellavato, Tiziana Squillaro, Stefania Del Gaudio, Giovanni Di Bernardo, Gianfranco Peluso, Mario De Rosa, Chiara Schiraldi, Umberto Galderisi
Mesenchymal stem cells, a subpopulation of mesenchymal stromal cells (MSCs), are present in the stroma of several tissues. MSC in vitro cultivation for clinical treatments may greatly affect MSC properties. A primary handicap is replicative senescence that impairs MSC functions. Hyaluronan (HA) is present in the extracellular matrix that composes the stem cell niche environment and is under investigation as a key factor for in vitro stem cell growth. We evaluated the effect on MSC cultivation of HA hybrid cooperative complexes (HCC) that are obtained from high (H) and low (L) weight molecules (NAHYCO™)...
July 12, 2018: Aging
Naohiro Seo, Kazunari Akiyoshi, Hiroshi Shiku
Exosomes are representative extracellular vesicles (EV) derived from multivesicular endosomes (MVE) and have been described as new particles in the communication of neighborhood and/or distant cells by serving as vehicles for transfer between cells of membrane and cytosolic proteins, lipids, and nucleotides including micro (mi) RNAs. Exosomes from immune cells and tumor cells act in part as a regulator in tumor immunology. CD8+ T cells that show potent cytotoxic activity against tumor cells reside as an inactive naïve form in the T-cell zone of secondary lymphoid organs...
July 12, 2018: Cancer Science
Ernestina M De Francesco, Marcello Maggiolini, Anna Maria Musti
The Notch signaling pathway acts in both physiological and pathological conditions, including embryonic development and tumorigenesis. In cancer progression, diverse mechanisms are involved in Notch-mediated biological responses, including angiogenesis and epithelial-mesenchymal-transition (EMT). During EMT, the activation of cellular programs facilitated by transcriptional repressors results in epithelial cells losing their differentiated features, like cell⁻cell adhesion and apical⁻basal polarity, whereas they gain motility...
July 10, 2018: International Journal of Molecular Sciences
X F Zhou, Q G Yan, X J Guo, X D Gou, J Q Han, J L Ye, H Y Zhang, F M Wang
Objective: To investigate the clinicopathologic features, immunophenotype, pathological diagnosis and treatment of malignant mixed tumor (MMT). Methods: Clinical and pathological features including immunohistochemical phenotypes were analyzed in a case of MMT accompanied with eccrine porocarcinoma (EP) involving both hands, diagnosed definitely in January 2018 along with review of relevant literature. Results: A 64-year-old man presented with multiple rash on both hands for 4 years. Three lesions of 0.5 to 2...
July 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
A K Roseweir, C Y Kong, J H Park, Lindsay Bennett, A G M T Powell, J Quinn, H C van Wyk, P G Horgan, D C McMillan, Joanne Edwards, C S Roxburgh
It is increasingly appreciated that host factors within the tumor centre and microenvironment play a key role in dictating colorectal cancer (CRC) outcomes. As a result, the metastatic process has now been defined as a result of epithelial-mesenchymal transition (EMT). Establishment of the role of EMT within the tumor centre and its effect on the tumor microenvironment would be beneficial for prognosis and therapeutic intervention in CRC. The present study assessed five immunohistochemical EMT markers within the tumor centre on a 185 stage II/III CRC patient TMA...
July 11, 2018: International Journal of Cancer. Journal International du Cancer
Daphne L van der Velden, Julia M Houthuijzen, Jeanine M L Roodhart, Erik van Werkhoven, Emile E Voest
Mesenchymal stem cells (MSCs) can play a vital role in tumor progression and anti-cancer therapy response, as demonstrated by various in vitro and in vivo model systems. Their ability to home to developing tumors and modulate the tumor microenvironment, by suppressing T-cell responses and contributing to the tumor stroma, is suggested to have a significant impact on disease progression, metastasis formation and therapy response. Most evidence, however, is derived from artificial models using exogenously administered MSCs...
July 11, 2018: International Journal of Cancer. Journal International du Cancer
Jun Chen, Lin Ma, Nianzhao Zhang, Yaofeng Zhu, Keqin Zhang, Zhishun Xu, Qian Wang
Background and aim: Mesenchymal stem cells (MSCs) are capable of impacting tumor progression but its role in tumor stroma remodeling still remains unclear. This present study was aimed to evaluate the potential function of MSCs on tumor stroma remodeling using rabbits VX2 bladder tumor model. Methods: The VX2 bladder tumor models were established by injecting mixed cell suspensions (106 of VX2 tumor cells and 0/106 /107 of autologous MSCs in group A, B, C, respectively) into the bladder mucosa using thirty male New Zealand white rabbits...
2018: International Journal of Biological Sciences
Janine Hensel, Antoinette Wetterwald, Ramzi Temanni, Irene Keller, Carsten Riether, Gabri van der Pluijm, Marco G Cecchini, George N Thalmann
Prostate and breast cancers frequently metastasize to bone. The physiological bone homeostasis is perturbed once cancer cells proliferate at the bone metastatic site. Tumors are complex structures consisting of cancer cells and numerous stroma cells. In this study, we show that osteolytic cancer cells (PC-3 and MDA-MB231) induce transcriptome changes in the bone/bone marrow microenvironment (stroma). This stroma transcriptome differs from the previously reported stroma transcriptome of osteoinductive cancer cells (VCaP)...
June 22, 2018: Oncotarget
Iftikhar Ali Khawar, Jong Kook Park, Eun Sun Jung, Myung Ah Lee, Suhwan Chang, Hyo-Jeong Kuh
Interactions between cancer cells and cancer-associated fibroblasts (CAFs) within the tumor microenvironment (TME) play an important role in promoting the profibrotic microenvironment and epithelial-mesenchymal transition (EMT), resulting in tumor progression and drug resistance in hepatocellular carcinoma (HCC). In the present study, we developed a mixed-cell spheroid model using Huh-7 HCC cells and LX-2 stellate cells to simulate the in vivo tumor environment with respect to tumor-CAF interactions. Spheroids were cultured from cancer cells alone (monospheroids) or as a mixture (mixed-cell spheroids) in ultra-low-attachment plates...
August 2018: Neoplasia: An International Journal for Oncology Research
Yoichiro Ikeda, Zhao Sun, Xiao Ru, Luk H Vandenberghe, Benjamin D Humphreys
BACKGROUND: After injury, mesenchymal progenitors in the kidney interstitium differentiate into myofibroblasts, cells that have a critical role in kidney fibrogenesis. The ability to deliver genetic material to myofibroblast progenitors could allow new therapeutic approaches to treat kidney fibrosis. Preclinical and clinical studies show that adeno-associated viruses (AAVs) efficiently and safely transduce various tissue targets in vivo ; however, protocols for transduction of kidney mesenchymal cells have not been established...
July 5, 2018: Journal of the American Society of Nephrology: JASN
Malgorzata A Komor, Linda Jw Bosch, Gergana Bounova, Anne S Bolijn, Pien Delis van-Diemen, Christian Rausch, Youri Hoogstrate, Andrew P Stubbs, Mark de Jong, Guido Jenster, Nicole Ct van Grieken, Beatriz Carvalho, Lodewyk Fa Wessels, Connie R Jimenez, Remond Ja Fijneman, Gerrit A Meijer, Natasja Dits, René Böttcher, Annemieke C Hiemstra, Bauke Ylstra, Daoud Sie, Evert van den Broek, David van der Meer, Floor Pepers, Eric Caldenhoven, Bart Janssen, Wilbert van Workum, Stef van Lieshout, Chris H Bangma, Geert van Leenders, Harmen van de Werken
Consensus molecular subtyping (CMS) is an RNA expression-based classification system for colorectal cancer (CRC). Genomic alterations accumulate during CRC pathogenesis, including the premalignant adenoma stage, leading to changes in RNA-expression. Only a minority of adenomas progress to malignancies, a transition associated with specific DNA copy number aberrations or microsatellite instability (MSI). We aimed to investigate whether colorectal adenomas can already be stratified into CMS classes, and whether specific CMS classes are related to presence of specific DNA copy number aberrations associated with progression to malignancy...
July 3, 2018: Journal of Pathology
Pratibha Sharma Shukla, Khushbakhat Mittal
CONTEXT: - Inflammatory myofibroblastic tumor is a mesenchymal neoplasm of low malignant potential. It was first described in lung, but is known to occur in many extrapulmonary sites including female genital organs, most commonly the uterus. It has a high recurrence rate and a low risk for metastasis. A more recently described aggressive variant, epithelioid myofibroblastic sarcoma with a predilection for the abdominal cavity of males, has also been recently reported to occur in ovary...
July 2, 2018: Archives of Pathology & Laboratory Medicine
Virgínea de Araújo Farias, Ana Belén Carrillo-Gálvez, Francisco Martín, Per Anderson
Multipotent mesenchymal stromal cells (MSCs) represent a promising cell-based therapy in regenerative medicine and for the treatment of inflammatory/autoimmune diseases. Importantly, MSCs have emerged as an important contributor to the tumor stroma with both pro- and anti-tumorigenic effects. However, the successful translation of MSCs to the clinic and the prevention of their tumorigenic and metastatic effect require a greater understanding of factors controlling their proliferation, differentiation, migration and immunomodulation in vitro and in vivo...
June 13, 2018: Cytokine & Growth Factor Reviews
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