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https://www.readbyqxmd.com/read/28911317/generation-of-ips-derived-model-cells-for-analyses-of-hair-shaft-differentiation
#1
Takumi Kido, Tomoatsu Horigome, Minori Uda, Naoki Adachi, Yohei Hirai
Biological evaluation of hair growth/differentiation activity in vitro has been a formidable challenge, primarily due to the lack of relevant model cell systems. To solve this problem, we generated a stable model cell line in which successive differentiation via epidermal progenitors to hair components is easily inducible and traceable. Mouse induced pluripotent stem (iPS) cell-derived cells were selected to stably express a tetracycline (Tet)-inducible bone morphogenic protein-4 (BMP4) expression cassette and a luciferase reporter driven by a hair-specific keratin 31 gene (krt31) promoter (Tet-BMP4-KRT31-Luc iPS)...
September 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28910979/crispr-cas9-based-efficient-genome-editing-in-staphylococcus-aureus
#2
Qi Liu, Yu Jiang, Lei Shao, Ping Yang, Bingbing Sun, Sheng Yang, Daijie Chen
Staphylococcus aureus is an important pathogenic bacterium prevalent in nosocomial infections and associated with high morbidity and mortality rates, which arise from the significant pathogenicity and multi-drug resistance. However, the typical genetic manipulation tools used to explore the relevant molecular mechanisms of S. aureus have multiple limitations: leaving a scar in the genome, comparatively low gene-editing efficiency, and prolonged experimental period. Here, we present a single-plasmid based on the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system which allows rapid and efficient chromosomal manipulation in S...
September 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28910723/sorption-desorption-equilibrium-and-diffusion-of-tetracycline-in-poultry-litter-and-municipal-biosolids-soil-amendments
#3
E D'Angelo
Tetracycline (TET) is commonly used to treat bacterial diseases in humans and chickens (Gallus gallus domesticus), is largely excreted, and is found at elevated concentrations in treated sewage sludge (biosolids) and poultry litter (excrement plus bedding materials). Routine application of these nutrient-and carbon-enriched materials to soils improves fertility and other characteristics, but the presence of antibiotics (and other pharmaceuticals) in amendments raises questions about potential adverse effects on biota and development of antibiotic resistance in the environment...
September 8, 2017: Chemosphere
https://www.readbyqxmd.com/read/28910443/spatiotemporally-controlled-ablation-of-klf5-results-in-dysregulated-epithelial-homeostasis-in-adult-mouse-corneas
#4
Chelsea L Loughner, Anil Tiwari, Doreswamy Kenchegowda, Sudha Swamynathan, Shivalingappa K Swamynathan
Purpose: Corneal epithelial (CE) homeostasis requires coordination between proliferation and differentiation. Here we examine the role of cell proliferation regulator Krüppel-like factor 5 (Klf5) in adult mouse CE homeostasis. Methods: Klf5 was ablated in a spatiotemporally restricted manner by inducing Cre expression in 8-week-old ternary transgenic Klf5LoxP/LoxP/Krt12rtTA/rtTA/Tet-O-Cre (Klf5Δ/ΔCE) mouse CE by administering doxycycline via chow. Normal chow-fed ternary transgenic siblings served as controls...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28910387/epstein-barr-virus-latent-membrane-protein-1-enhances-dendritic-cell-therapy-lymph-node-migration-activation-and-il-12-secretion
#5
James M Termini, Sachin Gupta, Francesca N Raffa, Elizabeth Guirado, Margaret A Fischl, Liguo Niu, Saravana Kanagavelu, Geoffrey W Stone
Dendritic cells (DC) are a promising cell type for cancer vaccines due to their high immunostimulatory capacity. However, improper maturation of DC prior to treatment may account for the limited efficacy of DC vaccine clinical trials. Latent Membrane Protein-1 (LMP1) of Epstein-Barr virus was examined for its ability to mature and activate DC as a gene-based molecular adjuvant for DC vaccines. DC were transduced with an adenovirus 5 vector (Ad5) expressing LMP1 under the control of a Tet-inducible promoter...
2017: PloS One
https://www.readbyqxmd.com/read/28904565/class-1-integrons-and-plasmid-mediated-multiple-resistance-genes-of-the-campylobacter-species-from-pediatric-patient-of-a-university-hospital-in-taiwan
#6
Yi-Chih Chang, Ni Tien, Jai-Sing Yang, Chi-Cheng Lu, Fuu-Jen Tsai, Tsurng-Juhn Huang, I-Kuan Wang
BACKGROUND: The Campylobacter species usually causes infection between humans and livestock interaction via livestock breeding. The studies of the Campylobacter species thus far in all clinical isolates were to show the many kinds of antibiotic phenomenon that were produced. Their integrons cause the induction of antibiotic resistance between bacterial species in the Campylobacter species. RESULTS: The bacterial strains from the diarrhea of pediatric patient which isolated by China Medical University Hospital storage bank...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28904263/concerning-increase-in-antimicrobial-resistance-in-shiga-toxin-producing-escherichia-coli-isolated-from-young-animals-during-1980-2016
#7
Flore Chirila, Alexandra Tabaran, Nicodim Fit, George Nadas, Marian Mihaiu, Flaviu Tabaran, Cornel Cătoi, Oana Lucia Reget, Sorin Daniel Dan
This study was conducted in order to assess the antimicrobial resistance patterns of E. coli isolated from young animals affected between 1980 and 2016. The selected isolates for this study (n=175) carried stx1/stx2 genes and the most prevalent type of pathogenic E. coli found belonged to serogroup O101, antigen (K99)-F41 positive. All STEC-positive isolates were tested for susceptibility to 11 antimicrobials. Multidrug resistance (MDR) increased from 11% during the 1980s to 40% between 2000 and 2016. Resistance to tetracycline and streptomycin was the most frequent co-resistance phenotype (37%)...
September 12, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28900741/occurrence-and-distribution-of-antibiotic-resistance-genes-in-water-supply-reservoirs-in-jingjinji-area-china
#8
Kai Zhang, Zhi-Guang Niu, Zhiwei Lv, Ying Zhang
Jingjinji area occupies important position in developing of the Chinese economy, while there exists a sharp conflict between economic growth and limited water resources in this area. To ensure the safety of water consumption of cities in Jingjinji area, we investigated the abundance of three classes of antibiotic resistance genes (ARGs) in water and sediment of six water supply reservoirs in this area. The results showed that the detection frequency of sul1, tetM and ermB were 100%. However, the content ranges of these genes were different (10(-5) to 10(-2)/16S gene copies for sul1, 10(-5) to 10(-3)/16S gene copies for ermB, and 10(-5) to 10(-3)/16S gene copies for tetM)...
September 12, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28900542/mitochondrial-dna-haplotypes-induce-differential-patterns-of-dna-methylation-that-result-in-differential-chromosomal-gene-expression-patterns
#9
William T Lee, Xin Sun, Te-Sha Tsai, Jacqueline L Johnson, Jodee A Gould, Daniel J Garama, Daniel J Gough, Matthew McKenzie, Ian A Trounce, Justin C St John
Mitochondrial DNA copy number is strictly regulated during development as naive cells differentiate into mature cells to ensure that specific cell types have sufficient copies of mitochondrial DNA to perform their specialised functions. Mitochondrial DNA haplotypes are defined as specific regions of mitochondrial DNA that cluster with other mitochondrial sequences to show the phylogenetic origins of maternal lineages. Mitochondrial DNA haplotypes are associated with a range of phenotypes and disease. To understand how mitochondrial DNA haplotypes induce these characteristics, we used four embryonic stem cell lines that have the same set of chromosomes but possess different mitochondrial DNA haplotypes...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28900196/characterization-of-a-new-pathway-that-activates-lumisterol-in-vivo-to-biologically-active-hydroxylumisterols
#10
Andrzej T Slominski, Tae-Kang Kim, Judith V Hobrath, Zorica Janjetovic, Allen S W Oak, Arnold Postlethwaite, Zongtao Lin, Wei Li, Yukimasa Takeda, Anton M Jetten, Robert C Tuckey
Using LC/qTOF-MS we detected lumisterol, 20-hydroxylumisterol, 22-hydroxylumisterol, 24-hydroxylumisterol, 20,22-dihydroxylumisterol, pregnalumisterol, 17-hydroxypregnalumisterol and 17,20-dihydroxypregnalumisterol in human serum and epidermis, and the porcine adrenal gland. The hydroxylumisterols inhibited proliferation of human skin cells in a cell type-dependent fashion with predominant effects on epidermal keratinocytes. They also inhibited melanoma proliferation in both monolayer and soft agar. 20-Hydroxylumisterol stimulated the expression of several genes, including those associated with keratinocyte differentiation and antioxidative responses, while inhibiting the expression of others including RORA and RORC...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28898804/application-of-manure-containing-tetracyclines-slowed-down-the-dissipation-of-tet-resistance-genes-and-caused-changes-in-the-composition-of-soil-bacteria
#11
Wenguang Xiong, Mei Wang, Jinjun Dai, Yongxue Sun, Zhenling Zeng
Manure application contributes to the increased environmental burden of antibiotic resistance genes (ARGs). We investigated the response of tetracycline (tet) resistance genes and bacterial taxa to manure application amended with tetracyclines over two months. Representative tetracyclines (oxytetracycline, chlorotetracycline and doxycycline), tet resistance genes (tet(M), tet(O), tet(W), tet(S), tet(Q) and tet(X)) and bacterial taxa in the untreated soil, +manure, and +manure+tetracyclines groups were analyzed...
September 9, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28895735/reduction-sensitive-paclitaxel-prodrug-self-assembled-nanoparticles-with-tetrandrine-effectively-promote-synergistic-therapy-against-drug-sensitive-and-multidrug-resistant-breast-cancer
#12
Mengjuan Jiang, Ruoshi Zhang, Yingli Wang, Wenna Jing, Ying Liu, Yan Ma, Bingjun Sun, Menglin Wang, Peizhuo Chen, Hongzhuo Liu, Zhonggui He
Co-delivery of multiple drugs with complementary anticancer mechanisms by nano-carriers offers an effective strategy to treat cancers. Herein, conjugation (PTX-SS-VE) of paclitaxel (PTX) to vitamin E succinate (VE) self-assembled nanoparticles were used to load tetrandrine (TET) for combinational treatment against breast carcinoma. The ratio of PTX-SS-VE and TET was optimized. Compared with PTX, the TET/PTX-SS-VE co-loaded nanoparticles (TPNPs) demonstrated superior cytotoxicity against both MCF-7 cells and MCF-7/Adr cells...
September 12, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28894047/the-role-of-vitamin-c-in-epigenetic-regulation
#13
Jolanta Guz, Ryszard Oliński
Vitamin C (L-ascorbic acid) is a micronutrient best known for its anti-scurvy activity in humans. Vitamin C is involved in many biological processes involving enzymatic reactions that are catalyzed by members of dioxygenases which use Fe(II) and 2-oxoglutarate as a co-substrate.The article reviews recent data that suggest the involvement of ascorbate in dioxygenases catalyzed chromatin and DNA modifications which thereby contribute to epigenetic regulation. Concerning chromatin modification, the dioxygenases are involved in distinct demethylation reactions with varying specificity for the position of the lysine on the target histone...
August 24, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28892700/modification-of-biochar-derived-from-sawdust-and-its-application-in-removal-of-tetracycline-and-copper-from-aqueous-solution-adsorption-mechanism-and-modelling
#14
Yaoyu Zhou, Xiaocheng Liu, Yujia Xiang, Pei Wang, Jiachao Zhang, Fengfeng Zhang, Jianhong Wei, Lin Luo, Ming Lei, Lin Tang
Highly efficient simultaneous removal of Cu(II) and tetracycline (TET) from aqueous solution was accomplished by iron and zinc doped sawdust biochar (Fe/Zn-biochar). The mutual effects and inner mechanisms of their adsorption onto Fe/Zn-biochar were systematically investigated via sole and binary systems, sorption isotherm and adsorption kinetics models. The liquid-film diffusion step might be the rate-limiting step for tetracycline, the interaction of Cu(II) was more likely controlled by both intra particle diffusion model and liquid film diffusion model...
September 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28889481/a-review-of-the-biologic-and-clinical-significance-of-genetic-mutations-in-angioimmunoblastic-t-cell-lymphoma
#15
REVIEW
Kota Fukumoto, Tran B Nguyen, Shigeru Chiba, Mamiko Sakata-Yanagimoto
Angioimmunoblastic T-cell lymphoma (AITL) is an age-related malignant lymphoma, characterized by immune system-dysregulated symptoms. Recent sequencing studies have clarified the recurrent mutations in ras homology family member A (RHOA) and in genes encoding epigenetic regulators, tet methyl cytosine dioxygenase 2 (TET2), DNA methyl transferase 3 alpha (DNMT3A), and isocitrate dehydrogenase 2, mitochondrial (IDH2), as well as those related to the T-cell receptor signaling pathway in AITL. In this review, we will focus on how this genetic information has changed the understanding of the developmental process of AITL and will in future lead to individualized therapies for AITL patients...
September 10, 2017: Cancer Science
https://www.readbyqxmd.com/read/28888881/prevalence-and-genetic-profiling-of-tetracycline-resistance-tet-r-genes-and-transposable-element-tn916-in-environmental-enterococcus-species
#16
Sindhu Zahid, Hassan Bin-Asif, Khwaja Ali Hasan, Marium Rehman, Syed Abid Ali
Resistance against antimicrobial agents in enterococci is a global concern that not only challenges infection therapy but also make them reservoir of antibiotic resistance in human and animal alike. This study was conducted to establish tetracycline resistance profiles, prevalence of tet genes and transposable element (Tn916) in enterococcal soil and clinical isolates. Enterococci (n = 1210) from different environmental niche were collected and subjected to molecular identification. In total, 361 isolates showed tetracycline resistance at the breakpoint of 32 μg ml(-1)...
September 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28888643/nasal-carriage-of-methicillin-resistant-coagulase-negative-staphylococci-in-healthy-humans-is-associated-with-occupational-pig-contact-in-a-dose-response-manner
#17
Ling Li, Zhiyao Chen, Dan Guo, Shunming Li, Jingya Huang, Xiaolin Wang, Zhenjiang Yao, Sidong Chen, Xiaohua Ye
This study aimed to explore the association between occupational pig contact and human methicillin-resistant coagulase-negative staphylococci (MRCoNS) carriage. We conducted a cross-sectional study of pig exposed participants and controls in Guangdong, China, using a multi-stage sampling design. Participants provided a nasal swab for MRCoNS analysis and resulting isolates were tested for antibiotic susceptibility. The dose-response relation was examined using log binomial regression or Poisson regression models...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28888623/effect-of-different-oral-oxytetracycline-treatment-regimes-on-selection-of-antimicrobial-resistant-coliforms-in-nursery-pigs
#18
Ana Herrero-Fresno, Camilla Zachariasen, Nanna Nørholm, Anders Holm, Lasse Engbo Christiansen, John Elmerdahl Olsen
A major concern derived from using antimicrobials in pig production is the development of resistance. This study aimed to assess the impact of selected combinations of oral dose and duration of treatment with oxytetracycline (OTC) on selection of tetracycline resistant (TET-R) coliforms recovered from swine feces. The work encompassed two studies: 1) OTC 5mg/kg and 20mg/kg were administered to nursery pigs for 3 and 10days, respectively, under controlled experimental conditions, and 2) 10mg/kg, 20mg/kg and 30mg/kg OTC were given to a higher number of pigs for 6, 3 and 2days, respectively, under field conditions...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28888139/tet-proteins-in-natural-and-induced-differentiation
#19
REVIEW
James P Scott-Browne, Chan-Wang J Lio, Anjana Rao
The ten-eleven-translocation (TET) proteins oxidize 5-methylcytosine in DNA. Alterations in TET protein function have been linked to cancer, but TETs have also been observed to influence many cell differentiation processes. Here we review recent work assessing the contribution of TET proteins to natural and induced differentiation. Altogether these analyses have helped characterize how TETs and their enzymatic products influence DNA methylation patterns, regulatory element activity, DNA binding protein specificity and gene expression...
September 6, 2017: Current Opinion in Genetics & Development
https://www.readbyqxmd.com/read/28887421/impact-of-raised-without-antibiotics-beef-cattle-production-practices-on-occurrences-of-antimicrobial-resistance
#20
Amit Vikram, Pablo Rovira, Getahun E Agga, Terrance M Arthur, Joseph M Bosilevac, Tommy L Wheeler, Paul S Morley, Keith E Belk, John W Schmidt
The specific antimicrobial resistance (AMR) decreases that can be expected from reducing antimicrobial (AM) use in United States (US) beef production have not been defined. To address this data gap, feces were recovered from 36 lots of "raised without antibiotics" (RWA) and 36 lots of "conventional" (CONV) beef cattle. Samples (N = 719) were collected during harvest and distributed over a year. AMR was assessed by: i) culture of six AM-resistant bacteria (ARB); ii) qPCR for 10 AMR genes (ARGs); iii) an qPCR array of 84 ARGs; and iv) metagenomic sequencing...
September 8, 2017: Applied and Environmental Microbiology
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