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https://www.readbyqxmd.com/read/28428944/the-status-of-assisted-reproductive-technology-in-korea-in-2012
#1
Gyoung Hoon Lee, Hyun Jin Song, Young Min Choi, Hyuck Dong Han
OBJECTIVE: This study was designed to report the status of assisted reproductive technology (ART) therapy in South Korea between January 1, 2012 and December 31, 2012. METHODS: A localized online survey, originally developed by the International Committee Monitoring Assisted Reproductive Technologies, was first launched and provided to all available ART centers via email in 2015. Fresh embryo transfer (FET) cases were categorized as standard in vitro fertilization, intracytoplasmic sperm injection (ICSI), or half-ICSI...
March 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28428825/comprehensive-evaluation-of-genome-wide-5-hydroxymethylcytosine-profiling-approaches-in-human-dna
#2
Ksenia Skvortsova, Elena Zotenko, Phuc-Loi Luu, Cathryn M Gould, Shalima S Nair, Susan J Clark, Clare Stirzaker
BACKGROUND: The discovery that 5-methylcytosine (5mC) can be oxidized to 5-hydroxymethylcytosine (5hmC) by the ten-eleven translocation (TET) proteins has prompted wide interest in the potential role of 5hmC in reshaping the mammalian DNA methylation landscape. The gold-standard bisulphite conversion technologies to study DNA methylation do not distinguish between 5mC and 5hmC. However, new approaches to mapping 5hmC genome-wide have advanced rapidly, although it is unclear how the different methods compare in accurately calling 5hmC...
2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/28428226/rapid-throughput-analysis-of-gabaa-receptor-subtype-modulators-and-blockers-using-disbac1-3-membrane-potential-red-dye
#3
Atefeh Mousavi Nik, Brandon Pressly, Vikrant Singh, Shane Antrobus, Susan Hulsizer, Michael A Rogawski, Heike Wulff, Isaac N Pessah
Fluorometric Imaging Plate Reader (FLIPR®) membrane potential dye (FMP-Red-Dye) is a proprietary tool for basic discovery and high throughput drug screening for G-protein coupled receptors and ion channels. We optimized and validated this potentiometric probe to assay functional modulators of heterologous expressed GABAA receptor (GABAAR) isoforms (synaptic α1β3γ2, extrasynaptic α4β3δ, and β3 homopentomers). High-resolution mass spectrometry identified FMP-Red-dye as DisSBAC1(3). GABAAR expressing cells equilibrated with FMP-Red-Dye exhibited depolarized equilibrium membrane (Em) potentials compared to GABAAR-null cells...
April 20, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28411375/development-of-tetramethylenedisulfotetramine-tets-hapten-library-synthesis-electrophysiological-studies-and-immune-response-in-rabbits
#4
Bogdan Barnych, Natalia Vasylieva, Tom Joseph, Susan Hulsizer, Hai Nguyen, Tomas Cajka, Isaac Pessah, Heike Wulff, Shirley Gee, Bruce Hammock
There is a need for fast detection methods for the banned rodenticide tetramethylenedisulfotetramine (TETS), a highly potent blocker of the γ-aminobutyric acid (GABAA) receptors. General synthetic approach toward two groups of analogues was developed. Screening of the resulting library of compounds by FLIPR or whole-cell voltage-clamp revealed that despite the structural differences some of the TETS analogues retained GABAA receptor inhibition, however their potency was an order of magnitude lower. Antibodies raised in rabbits against some of the TETS analogues conjugated to protein recognized free TETS and will be used for the development of an immunoassay for TETS...
April 14, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28409505/dynamic-changes-in-dna-demethylation-in-the-tree-shrew-tupaia-belangeri-chinensis-brain-during-postnatal-development-and-aging
#5
Shu Wei, Hai-Rong Hua, Qian-Quan Chen, Ying Zhang, Fei Chen, Shu-Qing Li, Fan Li, Jia-Li Li
Brain development and aging are associated with alterations in multiple epigenetic systems, including DNA methylation and demethylation patterns. Here, we observed that the levels of the 5-hydroxymethylcytosine (5hmC) ten-eleven translocation (TET) enzyme-mediated active DNA demethylation products were dynamically changed and involved in postnatal brain development and aging in tree shrews (Tupaia belangeri chinensis). The levels of 5hmC in multiple anatomic structures showed a gradual increase throughout postnatal development, whereas a significant decrease in 5hmC was found in several brain regions in aged tree shrews, including in the prefrontal cortex and hippocampus, but not the cerebellum...
March 18, 2017: Zoological Research
https://www.readbyqxmd.com/read/28408905/tet-methylcytosine-oxidases-in-t-cell-and-b-cell-development-and-function
#6
REVIEW
Ageliki Tsagaratou, Chan-Wang J Lio, Xiaojing Yue, Anjana Rao
DNA methylation is established by DNA methyltransferases and is a key epigenetic mark. Ten-eleven translocation (TET) proteins are enzymes that oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and further oxidization products (oxi-mCs), which indirectly promote DNA demethylation. Here, we provide an overview of the effect of TET proteins and altered DNA modification status in T and B cell development and function. We summarize current advances in our understanding of the role of TET proteins and 5hmC in T and B cells in both physiological and pathological contexts...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28407691/tet2-and-meg3-promoter-methylation-is-associated-with-acute-myeloid-leukemia-in-a-hainan-population
#7
Hongxia Yao, Mengling Duan, Lie Lin, Congming Wu, Xiangjun Fu, Hua Wang, Li Guo, Wenting Chen, Li Huang, Dan Liu, Ruo Rao, Shuwen Wang, Yipeng Ding
The promoter of MEG3, which encodes the long non-coding RNA (lncRNA) MEG3, is often hypermethylated in acute myeloid leukemia (AML). Additionally, the Tet methylcytosine dioxygenase 2 gene (TET2) is frequently inactivated, which can lead to impaired DNA methylation and promote AML development. We examined the association between TET2 and MEG3 promoter hypermethylation in Hainan patients with AML. The expression of MEG3, TET2, miR-22-3p, and miR-22-5p was assessed in bone marrow samples from AML patients and healthy controls using real-time quantitative PCR...
March 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28402601/complementary-lock-and-key-ligand-binding-of-a-triplet-transmitter-to-a-nanocrystal-photosensitizer
#8
Xin Li, Alexander Fast, Zhiyuan Huang, Dmitry A Fishman, Ming Lee Tang
Owing to the difficulty in comprehensively characterizing nanocrystal (NC) surfaces, clear guidance for ligand design is lacking. In this work, a series of bidentate bis(pyridine) anthracene isomers (2,3-PyAn, 3,3-PyAn, 2,2-PyAn) that differ in their binding geometries were designed to find the best complementary fit to the NC surface. The efficiency of triplet energy transfer (TET) from the CdSe NC donor to a diphenylanthracene (DPA) acceptor mediated by these isomers was used as a proxy for the efficacy of orbital overlap and therefore ligand binding...
April 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28400889/evaluation-of-ocular-surface-disease-in-asian-patients-with-primary-angle-closure
#9
Tan Ee Ling, Khairuddin Othman, Ong Poh Yan, Rasdi Abdul Rashid, Cheong Min Tet, Azhany Yaakob, Liza-Sharmini Ahmad Tajudin
OBJECTIVE: To evaluate the incidence of ocular surface disease (OSD) and to determine the effects of topical pressure-lowering drugs on ocular surface disease in primary angle closure patients. METHODS: This was a cross-sectional comparative study comparing primary angle closure glaucoma (PACG) patients (Group A) with primary angle closure and primary angle closure suspect (Group B). Group A was treated with topical pressure-lowering drugs; Group B was not. Data on ocular diagnosis and details of treatment were obtained from medical records...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/28400429/genomic-analysis-of-thymic-epithelial-tumors-identifies-novel-subtypes-associated-with-distinct-clinical-features
#10
Hyun-Sung Lee, Hee-Jin Jang, Rohan Shah, David Yoon, Masatsugu Hamaji, Ori Wald, Ju-Seog Lee, David J Sugarbaker, Bryan M Burt
PURPOSE To reconcile the heterogeneity of thymic epithelial tumors (TETs) and gain deeper understanding of the molecular determinants of TETs, we set out to establish a clinically relevant molecular classification system for these tumors. <p>EXPERIMENTAL DESIGN Molecular subgrouping of TETs was performed in 120 patients from The Cancer Genome Atlas using a multidimensional approach incorporating analyses of DNA mutations, mRNA expression, and somatic copy number alterations (SCNA), and validated in two independent cohorts...
April 11, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28398746/sensitive-immunoassay-for-detection-and-quantification-of-the-neurotoxin-tetramethylenedisulfotetramine
#11
Natalia Vasylieva, Bogdan Barnych, Amy Rand, Bora Inceoglu, Shirley J Gee, Bruce D Hammock
Tetramethylenedisulfotetramine (TETS, tetramine) is a formerly used and highly neurotoxic rodenticide. Its lethality, recent history of intentional use for mass poisoning, and the absence of a known antidote raise public health concerns. Therefore, rapid, high throughput, and sensitive methods for detection and quantification of TETS are critical. Instrumental analysis method such as GC/MS is sensitive but not rapid or high throughput. Therefore, an immunoassay selective to TETS was developed. The assay shows an IC50 of 4...
April 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28396520/cytosine-modifications-modulate-the-chromatin-architecture-of-transcriptional-enhancers
#12
Elise A Mahé, Thierry Madigou, Aurélien A Sérandour, Maud Bizot, Stéphane Avner, Frédéric Chalmel, Gaëlle Palierne, Raphaël Métivier, Gilles Salbert
Epigenetic mechanisms are believed to play key roles in the establishment of cell-specific transcription programs. Accordingly, the modified bases 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) have been observed in DNA of genomic regulatory regions such as enhancers, and oxidation of 5mC into 5hmC by Ten Eleven Translocation (TET) proteins correlates with enhancer activation. However, the functional relationship between cytosine modifications and the chromatin architecture of enhancers remains elusive...
April 10, 2017: Genome Research
https://www.readbyqxmd.com/read/28395715/-subcellular-re-distribution-of-f-actin-in-mcf7-cells-upon-inducible-expression-of-cdkn2a-p16-ink4a
#13
Jianqing Liang, Manna Wang, Zhengrong Zhang, Long Yuan, You Zheng, Hongyan Huang, Qiang Sun, Jufang Wang, Xiaoning Wang
Objective To investigate the effect of CDKN2A/p16(INK4a), a cyclin-dependent kinase inhibitor, on cell morphology and cytoskeleton in MCF7 breast cancer cells by tetracycline operon (Tet-on) inducible gene expression system. Methods MCF7 cells, genetically null for p16(INK4a) expression, were sequentially infected with two viruses of Tet-on systems to make MCF7-pTet-on-p16(INK4a) cells, which were induced to express p16NK4a by doxycycline (Dox) treatment. Cell growth was evaluated by cell counting. To observe the effect of p16(INK4a) on cell adhesion and cytoskeleton, intercellular adhesion and intracellular F-actin were examined by fluorescent staining with anti-E-cadherin antibody and phalloidin, respectively...
April 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28393256/tetrandrine-inhibits-the-proliferation-of-human-osteosarcoma-cells-by-upregulating-the-pten-pathway
#14
Dong-Dong Tian, Ran-Xi Zhang, Nian Wu, Wei Yuan, Shuai-Hong Luo, Hou-Qing Chen, Yang Liu, Yang Wang, Bai-Cheng He, Zhong-Liang Deng
Tetrandrine (TET) is a natural product isolated from the Chinese herb Stephania tetrandra S. Moore and has been reported to have antiproliferation and apoptosis-inducing activity in various malignant tumor cells. However, the exact molecular mechanisms underlying these effects remain unclear. In the present study, we tested the antiproliferation effect of TET on osteosarcoma (OS) 143B cells and explored the possible potential molecular mechanism in this process. Using CCK-8 assay and flow cytometry, we found that TET inhibited proliferation, induced apoptosis and arrested the cell cycle of the 143B cells...
April 6, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28390956/the-structure-of-the-amorphous-matrix-of-keratins
#15
Murat Kadir, Xinwei Wang, Bowen Zhu, Jing Liu, Duane Harland, Crisan Popescu
Various keratin fibers, particularly human hairs, were investigated by transmission electron microscopy, TEM, solid-state 1HNMR and Transient Electro-Thermal Technique, TET. The results converge to suggest that the matrix of keratin fiber cortex, far from being amorphous, has a well-defined nano-scale grainy structure, the size of these grains being around 2 - 4 nm. The size of the grains appears to strongly depend on the chemical treatment of the fiber, on the temperature and on the relative humidity of the environment, as well as on the physiological factors at the level of fiber production in follicle...
April 5, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28390885/what-are-the-preoperative-outcome-measures-that-predispose-to-periprosthetic-fractures-after-primary-total-knee-arthroplasty
#16
Jason Beng Teck Lim, Hamid Rahmatullah Bin Abd Razak, Suraya Zainul-Abidin, John Carson Allen, Joyce Suang Bee Koh, Tet Sen Howe
BACKGROUND: There is little known in the literature about whether preoperative patient-reported outcome measures (PROMs) would affect the risk of periprosthetic fractures (PPFs) after primary total knee arthroplasty (TKA). Our study aims to evaluate the predictive values of PROMs on PPF after primary TKA. We hypothesize that poorer PROMs are associated with a higher risk of PPF after primary TKA. METHODS: We reviewed prospectively collected data in our hospital arthroplasty registry...
March 16, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28379186/establishment-of-the-inducible-tet-on-system-for-the-activation-of-the-silent-trichosetin-gene-cluster-in-fusarium-fujikuroi
#17
Slavica Janevska, Birgit Arndt, Leonie Baumann, Lisa Helene Apken, Lucas Maciel Mauriz Marques, Hans-Ulrich Humpf, Bettina Tudzynski
The PKS-NRPS-derived tetramic acid equisetin and its N-desmethyl derivative trichosetin exhibit remarkable biological activities against a variety of organisms, including plants and bacteria, e.g., Staphylococcus aureus. The equisetin biosynthetic gene cluster was first described in Fusarium heterosporum, a species distantly related to the notorious rice pathogen Fusarium fujikuroi. Here we present the activation and characterization of a homologous, but silent, gene cluster in F. fujikuroi. Bioinformatic analysis revealed that this cluster does not contain the equisetin N-methyltransferase gene eqxD and consequently, trichosetin was isolated as final product...
April 5, 2017: Toxins
https://www.readbyqxmd.com/read/28365311/clinical-impact-and-prevalence-of-mrsa-cc398-and-differences-between-mrsa-tet-r-and-mrsa-tet-s-in-an-area-of-spain-with-a-high-density-of-pig-farming-a-prospective-cohort-study
#18
E Reynaga, C Torres, M Garcia-Nuñez, M Navarro, A Vilamala, E Puigoriol, G E Lucchetti, M Sabrià
OBJETIVES: Tetracycline-resistance (Tet(R)) is a phenotypic marker of the livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) CC398 clone. The aim of this study was to analyze the prevalence of MRSA CC398 in patients in contact with healthcare facilities and differences between patients with MRSA-Tet(R) and MRSA tetracycline-susceptible (Tet(S)) strains. METHODS: Patients diagnosed with MRSA from January-2012 until December-2015 were divided into two groups, MRSA-Tet(R) and MRSA-Tet(S)...
March 29, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28364156/predicting-postoperative-complications-after-bariatric-surgery-the-bariatric-surgery-index-for-complications-basic
#19
Usha K Coblijn, Julian Karres, Christel A L de Raaff, Steve M M de Castro, Sjoerd M Lagarde, Willem F van Tets, H Jaap Bonjer, Bart A van Wagensveld
BACKGROUND: Around 20% of bariatric surgery patients develop a short- or long-term complication. OBJECTIVE: Aim of this study was to develop a risk model predicting complications: the Bariatric Surgery Index for Complications (BASIC). SETTING: The Obesity Center Amsterdam, located in a large teaching hospital, in Amsterdam, The Netherlands. METHODS: A prospective consecutive database including patients operated between November 2007 and February 2015 was used...
March 31, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28361400/comparison-of-two-modified-coal-ash-ferric-carbon-micro-electrolysis-ceramic-media-for-pretreatment-of-tetracycline-wastewater
#20
Kunlun Yang, Yang Jin, Qinyan Yue, Pin Zhao, Yuan Gao, Suqing Wu, Baoyu Gao
Application of modified sintering ferric-carbon ceramics (SFC) and sintering-free ferric-carbon ceramics (SFFC) based on coal ash and scrap iron for pretreatment of tetracycline (TET) wastewater was investigated in this article. Physical property, morphological character, toxic metal leaching content, and crystal component were studied to explore the application possibility of novel ceramics in micro-electrolysis reactors. The influences of operating conditions including influent pH, hydraulic retention time (HRT), and air-water ratio (A/W) on the removal of tetracycline were studied...
March 30, 2017: Environmental Science and Pollution Research International
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