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T H Johannsen, K M Main, M L Ljubicic, T K Jensen, H R Andersen, M S Andersen, J H Petersen, A-M Andersson, A Juul
CONTEXT: The early activation of the hypothalamic-pituitary-gonadal axis during infancy can be used in the evaluation of infants suspected of disorders of sex development (DSD). However, few data exists on sex-specific reference ranges for these hormones during early life. OBJECTIVE: To evaluate sex-differences in reproductive hormone concentrations in serum from healthy infants in order to define sex-specific cut-off values and to apply these in infants with DSD...
June 15, 2018: Journal of Clinical Endocrinology and Metabolism
Timothy S McHale, Wai-Chi Chee, Ka-Chun Chan, David T Zava, Peter B Gray
A large body of research links testosterone and cortisol to male-male competition. Yet, little work has explored acute steroid hormone responses to coalitional, physical competition during middle childhood. Here, we investigate testosterone, dehydroepiandrosterone (DHEA), androstenedione, and cortisol release among ethnically Chinese boys in Hong Kong (N = 102), aged 8-11 years, during a soccer match (n = 84) and an intrasquad soccer scrimmage (n = 81), with 63 participants competing in both treatments. The soccer match and intrasquad soccer scrimmage represented out-group and in-group treatments, respectively...
June 16, 2018: Human Nature: An Interdisciplinary Biosocial Perspective
Yu V Ukraintseva, K M Liaukovich, А A Polishchuk, О V Martynova, D A Belov, E S Simenel, М Meira E Cruz, А N Nizhnik
OBJECTIVES: Levels of steroid hormones such as androgens and cortisol exhibit circadian variation, and their fluctuations are related to the sleep-wake cycle. Currently, the functional role of different stages of sleep in steroid hormone secretion remains unclear. The present study aims to explore the effect of slow-wave sleep (SWS) suppression on morning levels of cortisol and androgens. METHODS: Twelve healthy male volunteers participated in two experimental sessions: a session with selective SWS suppression during night sleep and a session with regular night sleep (control)...
May 12, 2018: Sleep Medicine
Carina Ankarberg-Lindgren, Jovanna Dahlgren, Mats X Andersson
BACKGROUND: Androgen and estrogen determinations serve as important diagnostic markers in a variety of clinical conditions. However, one challenge is to enhance assay sensitivity for determination in the lowest range, such as in prepubertal children. We here present a recently developed gas chromatography-tandem mass spectrometry (GC-MS/MS) method for determination of androstenedione (A4 ), dihydrotestosterone (DHT), testosterone (T), estrone (E1 ), and estradiol (E2 ) in children, which we have compared with the sensitive radioimmunoassays; E2 extraction-RIA and T-RIA...
June 9, 2018: Journal of Steroid Biochemistry and Molecular Biology
Osama Adnan Kensara
Aims: The association between vitamin D and polycystic ovary syndrome (PCOS) is an active area of growing research. However, data in Saudi Arabia are scarce. This study aimed to define serum 25-hydroxyvitamin D (25(OH)D) levels among Saudi women with naïve PCOS, and to investigate the associations of their 25(OH)D status with their serum adiponectin and follistatin levels, along with indices of insulin resistance and hormonal deteriorations. Methods: In this case-control observational study, 63 women with PCOS and 65 age-and body mass index (BMI)-matched control women were assessed...
June 2018: Journal of Clinical & Translational Endocrinology
Heng Wang, Yong Pu, Lei Luo, Yunsheng Li, Yunhai Zhang, Zubing Cao
Excessive secretion of androgens including androstenedione and testosterone in theca cells frequently causes female infertility in mammals. Melatonin is a potent inhibitor of androgen production in gonadal cells of several species in a membrane receptor-dependent manner. However, the function of melatonin in steroidogenesis of porcine theca cells remains unclear. Here we report that melatonin inhibits androgen biosynthesis independently of its membrane receptors in pigs. Using flow cytometry, immunofluorescence and RT-PCR we showed that the vast majority of cells isolated from the theca layer of antral follicles are indeed theca cells...
June 1, 2018: Theriogenology
Abdul Basit, John K Amory, Bhagwat Prasad
Development of an oral testosterone (T) therapy has proven extremely challenging because of extensive and variable first-pass metabolism. We investigated the in vivo metabolism of T with increasing oral doses of T, both alone and with the co-administration of dutasteride (5α-reductase inhibitor) by LC-MS/MS. In eugonadal men prior to dosing, the circulating concentration of T, androstenedione, etiocholanolone-glucuronide, and androsterone-glucuronide was 8.6, 20.9, 9.1 and 55.3%, respectively, of the total T-related species, whereas T-glucuronide was ∼1%...
June 7, 2018: Clinical and Translational Science
Nguyen Thi Phuong Oanh, Teruhiko Kido, Seijiro Honma, Yuko Oyama, Le Thai Anh, Hoang Duc Phuc, Nguyen Hoang Viet, Ho Dung Manh, Rie Okamoto, Hideaki Nakagawa, Shoji F Nakayama, Dang Duc Nhu, Dao Van Tung, Vo Van Chi, Nguyen Hung Minh, Ngo Van Toan
Dioxins have been suspected to be potential substances causing endocrine disruptions in humans. We are conducting the research in one of three dioxin exposure areas (hotspots) in Vietnam. We previously reported that the salivary dehydroepiandrosterone (DHEA) level decreased in 3-year-old Vietnamese children and that it was significantly inversely correlated with polychlorinated dibenzodioxin/dibenzofuran levels in their mother's breast milk. In this study, we investigated the influence of exposure to dioxin on steroid hormone biosynthesis in the same children when they reached 5 years of age, focusing on androgens...
June 1, 2018: Science of the Total Environment
Rahel M Büttler, Esra Bagci, Henk S Brand, Martin den Heijer, Marinus A Blankenstein, Annemieke C Heijboer
BACKGROUND: Recently, measurements of steroids like testosterone, androstenedione, cortisol and cortisone in saliva are more and more applied in diagnostics and scientific studies. This is mainly due to the simple and non-invasive collection of saliva. We aimed to evaluate the optimal way to collect saliva for steroid hormone measurement. METHODS: We investigated in twenty volunteers whether there is a difference between steroid hormone concentrations in unstimulated and stimulated saliva collected while chewing, using cotton and synthetic Salivettes®, citric acid or chewing gum...
June 1, 2018: Steroids
Daniel Hettel, Ao Zhang, Mohammad Alyamani, Michael Berk, Nima Sharifi
3βHSD1 enzymatic activity is essential for synthesis of potent androgens from adrenal precursor steroids in prostate cancer. A germline variant in HSD3B1, the gene that encodes 3βHSD1, encodes for a stable enzyme, regulates adrenal androgen dependence, and is a predictive biomarker of poor clinical outcomes after gonadal testosterone deprivation therapy. However, little is known about HSD3B1 transcriptional regulation. Generally, it is thought that intratumoral androgen synthesis is up-regulated after gonadal testosterone deprivation, enabling development of castration-resistant prostate cancer (CRPC)...
May 29, 2018: Endocrinology
Joseph A Majzoub, Lisa Swartz Topor
We propose that the normal adrenarche-related rise in dehydroepiandrosterone (DHEA) secretion is ultimately caused by the rise in cortisol production occurring during childhood and adolescent growth, by the following mechanisms. (1) The onset of childhood growth leads to a slight fall in serum cortisol concentration due to growth-induced dilution and a decrease in the negative feedback of cortisol upon ACTH secretion. (2) In response, ACTH rises and stimulates increased cortisol synthesis and secretion in the growing body to restore the serum cortisol concentration to normal...
May 30, 2018: Hormone Research in Pædiatrics
Emma H Allott, Elizabeth M Masko, Alexis R Freedland, Everardo Macias, Kristine Pelton, Keith R Solomon, Elahe A Mostaghel, George V Thomas, Salvatore V Pizzo, Michael R Freeman, Stephen J Freedland
BACKGROUND: Some, but not all, epidemiologic evidence supports a role for cholesterol, the precursor for steroid hormone synthesis, in prostate cancer. Using a PTEN-null transgenic mouse model of prostate cancer, we tested the effect of modifying serum cholesterol levels on prostate tumor development and growth. We hypothesized that serum cholesterol reduction would lower tumor androgens and slow prostate cancer growth. METHODS: PTENloxP/loxP -Cre+ mice consuming ad libitum high fat, high cholesterol diets (40% fat, 1...
May 23, 2018: Prostate Cancer and Prostatic Diseases
Michal Mokáň, František Nehaj, Juraj Sokol, Peter Vaňuga, Marián Mokáň
Hypertestosteronism as part of hyperandrogenic states in women is generally defined as abundance of male hormones (in this case abundance of testosterone). Spectrum of clinical symptoms include menstrual disorders, amenorrhoea, different range of hirsutism and virilization. Statistically, most androgen secreting tumors are ovarian aetiology (testosterone secreting tumors located in suprarenal gland are very rare). This rare tumor may produce excess amounts of testosterone, as well as its precursor androstenedione...
2018: Vnitr̆ní Lékar̆ství
Catharina Olivius, Kerstin Landin-Wilhelmsen, Daniel S Olsson, Gudmundur Johannsson, Åsa Tivesten
PURPOSE: Women with hypopituitarism have increased morbidity and mortality, and hypogonadism has been suggested to be a contributing mechanism. The purpose of this study was to investigate the prevalence of central hypogonadism and hypoandrogenism in women with hypopituitarism at a single Swedish center. METHODS: All consecutive women (n = 184) who commenced growth hormone (GH) replacement therapy at Sahlgrenska University Hospital in Gothenburg between 1995 and 2015 were included...
May 22, 2018: Pituitary
Robyn E Ellerbrock, Igor F Canisso, Fabio S Lima, Clifford F Shipley, Donald L Thompson, Alan J Conley, Kevin H Kline
The objectives of this study were to determine the concentrations of reproductive and metabolic hormones during the peripubertal period and to assess their relationship with testicular development and body fat deposition. Blood samples were collected from 23 healthy Standardbred colts every four weeks for twelve months. Colts were weighed monthly, and percent of body fat and testicular volume estimated by ultrasound. Onset of puberty was determined as the month when testosterone was two standard deviations above the previous mean...
May 14, 2018: Theriogenology
Angelo Spinello, Matic Pavlin, Lorenzo Casalino, Alessandra Magistrato
Cytochromes P450 (CYP450) are involved in the metabolism of exogenous compounds and in the synthesis of signaling molecules. Among the latter, human aromatase (HA) promotes estrogen biosynthesis, being a key pharmacological target against breast cancers. After decades of debate, the interest in gaining a comprehensive picture of HA catalysis has been renewed by the recent discovery that Compound I (Cpd I) is the reactive species of the peculiar aromatization step. Here, we provide for the first time a complete atomic-level picture of all controversial steps of estrogen biosynthesis...
May 17, 2018: Chemistry: a European Journal
Piotr J Mak, Ruchia Duggal, Ilia G Denisov, Michael C Gregory, Stephen G Sligar, James R Kincaid
The multifunctional enzyme, cytochrome P450 (CYP17A1), plays a crucial role in the production of androgens, catalyzing two key reactions on pregnenolone (PREG) and progesterone (PROG), the first being a 17-hydroxylation to generate 17-OH PREG and 17-OH PROG, with roughly equal efficiencies. The second is a C-C bond scission or "lyase" reaction in which the C17-C20 bond is cleaved, leading to the eventual production of powerful androgens, whose involvement in the proliferation of prostate cancer has generated intense interest in developing inhibitors of CYP17A1...
May 15, 2018: Journal of the American Chemical Society
Ratchanok Pingaew, Veda Prachayasittikul, Nuttapat Anuwongcharoen, Supaluk Prachayasittikul, Somsak Ruchirawat, Virapong Prachayasittikul
A three series of thioureas, monothiourea type I (4a-g), 1,4-bisthiourea type II (5a-h) and 1,3-bisthiourea type III (6a-h) were synthesized. Their aromatase inhibitory activities have been evaluated. Interestingly, eight thiourea derivatives (4e, 5f-h, 6d, 6f-h) exhibited the aromatase inhibitory activities with IC50 range of 0.6-10.2 μM. The meta-bisthiourea bearing 4-NO2 group (6f) and 3,5-diCF3 groups (6h) were shown to be the most potent compounds with sub-micromolar IC50 values of 0.8 and 0.6 μM, respectively...
May 4, 2018: Bioorganic Chemistry
Ewa Kozłowska, Natalia Hoc, Jordan Sycz, Monika Urbaniak, Monika Dymarska, Jakub Grzeszczuk, Edyta Kostrzewa-Susłow, Łukasz Stępień, Elżbieta Pląskowska, Tomasz Janeczko
BACKGROUND: Steroid compounds are very interesting substrates for biotransformation due to their high biological activity and a high number of inactivated carbons which make chemical modification difficult. Microbial transformation can involve reactions which are complicated and uneconomical in chemical synthesis, and searching for a new effective biocatalyst is necessary. The best known entomopathogenic species used in steroid modification is Beauveria bassiana. In this study we tested the ability of Isaria farinosa, another entomopathogenic species, to transform several steroids...
May 12, 2018: Microbial Cell Factories
Chihiro Miyashita, Atsuko Araki, Takahiko Mitsui, Sachiko Itoh, Houman Goudarzi, Seiko Sasaki, Jumboku Kajiwara, Tsuguhide Hori, Kazutoshi Cho, Kimihiko Moriya, Nobuo Shinohara, Katsuya Nonomura, Reiko Kishi
BACKGROUND: Prenatal exposure to dioxin-like compounds (DLCs) irreversibly affects fetal reproductive and steroid hormone synthesis. OBJECTIVE: This study aimed to assess the relationships between maternal DLCs and cord blood reproductive and steroid hormones. METHODS: Participants in this study were pregnant women who enrolled in the Sapporo Cohort of the Hokkaido Study between 2002 and 2005. We quantified 29 DLCs during the 2nd and 3rd trimesters in maternal blood...
May 9, 2018: Environment International
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