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https://www.readbyqxmd.com/read/30554113/manilkara-zapota-l-p-royen-leaf-water-extract-triggered-apoptosis-and-activated-caspase-dependent-pathway-in-ht-29-human-colorectal-cancer-cell-line
#1
Bee Ling Tan, Mohd Esa Norhaizan
Manilkara zapota (L.) P. Royen (Family: Sapotaceae), commonly called as sapodilla, has been applied as traditional folk medicine for diarrhea and pulmonary infections. Conventional therapy in colorectal cancer is not likely effective due to undesirable outcomes. The anti-colon cancer properties of Manilkara zapota leaf water extract have yet to be investigated thus far. Therefore, our present study aimed to evaluate the ability to induce apoptosis and the underlying mechanisms of Manilkara zapota leaf water extract against human colorectal cancer (HT-29) cells...
December 13, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30553146/surface-plasmon-resonance-of-gold-nanoparticles-as-a-colorimetric-sensor-for-indirect-detection-of-cefixime
#2
Zeinab Masoudyfar, Shahla Elhami
This study reports a colorimetric sensor with excellent sensitivity to detect Cefixime base on gold nanoparticles. Cefixime is an antibiotic which has a wide range of applications in medicine. Cefixime did not change the surface plasmon resonance bond in gold nanoparticles solution; therefore, there was no change in the color solution of gold nanoparticles. The presence of Alizarin Red S in the system was necessary for the degradation of Cefixime, resulting in the aggregation of gold nanoparticles and a color change from red to blue...
December 4, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/30552515/magnetic-core-dual-functional-shell-nanocomposites-with-peroxidase-mimicking-properties-for-use-in-colorimetric-and-electrochemical-sensing-of-hydrogen-peroxide
#3
Yuqing Li, Jing Liu, Yingchun Fu, Qingji Xie, Yanbin Li
A self-sacrificing catalytic method is described for the preparation of magnetic core/dual-functional-shell nanocomposites composed of magnetite, gold and Prussian Blue (type Fe3 O4 @Au-PB). Two reaction pathways are integrated. The first involves chemical dissolution of Fe3 O4 (the self-sacrificing step) by acid to release ferrous ions which then reacts with hexacyanoferrate(IV) to generate PB in the proximity of the magntic nanoparticles (MNPs). The second involves the reduction of tetrachloroaurate by hydroxylamine to generate gold under the catalytic effect of the MNPs...
December 14, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/30551611/carbon-quantum-dots-prepared-with-chitosan-for-synthesis-of-cqds-aunps-for-iodine-ions-detection
#4
Juanjuan Song, Li Zhao, Yesheng Wang, Yun Xue, Yujia Deng, Xihui Zhao, Qun Li
Water-soluble and reductive carbon quantum dots (CQDs) were fabricated by the hydrothermal carbonization of chitosan. Acting as a reducing agent and stabilizer, the as-prepared CQDs were further used to synthesize gold nanoparticles (AuNPs). This synthetic process was carried out in aqueous solution, which was absolutely "green". Furthermore, the CQDs/AuNPs composite was used to detect iodine ions by the colorimetric method. A color change from pink to colorless was observed with the constant addition of I- ions, accompanied by a decrease in the absorbance of the CQDs/AuNPs composite...
December 13, 2018: Nanomaterials
https://www.readbyqxmd.com/read/30551301/filter-paper-grafted-with-epoxide-based-copolymer-brushes-for-activation-free-peptide-nucleic-acid-conjugation-and-its-application-for-colorimetric-dna-detection
#5
Malinee Leekrajang, Pornpen Sae-Ung, Tirayut Vilaivan, Voravee P Hoven
Epoxide-bearing filter paper was first prepared by surface-initiated reversible addition-fragmentation chain transfer (RAFT) copolymerization of glycidyl methacrylate (GMA) and poly(ethylene glycol)methacrylate (PEGMA). Without the need for activation step, the capture peptide nucleic acid (PNA) probes carrying a C-terminal lysine modification can be directly immobilized on the surface-grafted poly[glycidyl methacrylate-ran-poly(ethylene glycol)methacrylate] (P(GMA-ran-PEGMA)) through ring-opening of epoxide groups in the GMA repeating units by amino groups in the PNA's structure...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30551062/biosensing-methods-for-determination-of-creatinine-a-review
#6
REVIEW
C S Pundir, Parveen Kumar, Ranjana Jaiwal
Creatinine is a metabolic product of creatine phosphate in muscles, which provides energy to muscle tissues. Creatinine has been considered as indicator of renal function specifically after dialysis, thyroid malfunction and muscle damage. The normal level of creatinine in the serum and its excretion through urine in apparently healthy individuals is 45-140 μM and 0.8-2.0 gm/day respectively. The level of creatinine reaches >1000 μM in serum during renal, thyroid and kidney dysfunction or muscle disorder...
November 25, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30550906/the-sources-and-dynamics-of-fine-grained-sediment-degrading-the-freshwater-pearl-mussel-margaritifera-margaritifera-beds-of-the-river-torridge-devon-uk
#7
S Pulley, A Goubet, I Moser, S Browning, A L Collins
The Freshwater Pearl Mussel (Margaritifera margaritifera) is an endangered organism across its entire range. It has a complex life cycle and stringent habitat requirements and is therefore an indicator species for the general ecosystem health of host rivers. Whereas historical intensive pearl fishing contributed to population declines, excess nutrient and sediment loss associated with current land use pressures in host river catchments, including modern intensive farming practices, are now highlighted as primary contributory factors...
November 28, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30550712/unbiased-measurement-of-phosphate-and-phosphorus-speciation-in-surface-waters
#8
Vlastimil Packa, Stefanie Maedler, Todd Howell, Vadim Bostan, Ngan Diep, Robert J Tooley, Vasile I Furdui
Trace-level phosphate analysis and phosphorus speciation in surface water remained challenging due to adsorption and phosphate uptake by microorganisms. In this study a two-dimensional ion chromatography separation coupled to electrospray ionization high-resolution mass spectrometry (2D-IC-ESI-MS) allowed isotope dilution quantitation of phosphate with simultaneous analysis of eleven phosphate-containing metabolites and two inorganic condensed phosphates. Samples were collected from Lake St. Clair, Lake Ontario and Georgian Bay (ON, Canada)...
December 14, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/30550042/salivary-creatinine-and-urea-in-patients-with-end-stage-chronic-kidney-disease-could-not-be-used-as-diagnostic-biomarkers-for-the-effectiveness-of-dialysis-treatment
#9
Anna Nenova Nogalcheva, Desislava Konstantinova, Petia Pechalova
Introduction: End-stage chronic kidney disease (CKD) is characterized by kidney failure with the organ's functions reduced or lost completely, where the kidneys are incapable of filtering excess fluids. Renal replacement therapy may be provided by peritoneal dialysis, hemodialysis or renal transplantation. Among the key indicators for tracking patients' current status are urea and creatinine levels. Aim: The study analyzed saliva as a medium to detect and measure urea and creatinine levels in end-stage CKD patients as well as to use it as criteria for the effectiveness of the dialysis treatment by comparing salivary urea and creatinine levels with their blood levels...
December 2018: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/30549502/-leucocyte-esterase-as-a-reliable-diagnostic-cost-effective-and-fast-in-knee-infections
#10
S U Ceja-Picazo, S Fuentes-Figueroa, A H Rivera-Villa, A R Hernández-Salgado, R Torres-González, A Miguel-Pérez, J A Hernández-García, J M Pérez-Atanasio
BACKGROUND: The articular infection represents a challenge due to its complexity and its devastating effect when not treated promptly. We have various diagnostic studies: cultures, ESR, CRP, count of leukocytes, among others but none is specific, it takes more than 30 minutes to complete and require complex infrastructure. In this study we determine the sensitivity and specificity of the leukocyte esterase for detection of an infectious process joint in Mexican population. MATERIAL AND METHODS: From November 2015 to April 2016 was obtained synovial fluid from patients with diagnosis of knee infection with or without implant and without infection with degenerative pathology of the knee...
July 2018: Acta Ortopédica Mexicana
https://www.readbyqxmd.com/read/30547588/colorimetric-sensor-array-for-monitoring-co-and-ethylene
#11
Zheng Li, Kenneth S Suslick
Developing miniaturized and inexpensive detectors remains an important and practical goal for field-deployable monitoring of toxic gases and other bioactive volatiles. CO (a common toxic pollutant) and ethylene (the phytohormone primarily responsible for fruit ripening) share the capability of strong back-π-bonding to low-oxidation-state metal ions, which has proved important in the development of metal-ion-based sensors for these gases. We report herein cumulative colorimetric sensor arrays based on Pd(II)-silica porous microsphere sensors and their application as an optoelectronic nose for rapid colorimetric quantification of airborne CO and ethylene...
December 14, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30545099/magnetic-nanoparticle-based-biosensing-assay-quantitatively-enhances-acid-fast-bacilli-count-in-paucibacillary-pulmonary-tuberculosis
#12
Cristina Gordillo-Marroquín, Anaximandro Gómez-Velasco, Héctor J Sánchez-Pérez, Kasey Pryg, John Shinners, Nathan Murray, Sergio G Muñoz-Jiménez, Allied Bencomo-Alerm, Adriana Gómez-Bustamante, Letisia Jonapá-Gómez, Natán Enríquez-Ríos, Miguel Martín, Natalia Romero-Sandoval, Evangelyn C Alocilja
A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB)...
December 12, 2018: Biosensors
https://www.readbyqxmd.com/read/30544951/sensory-polymeric-foams-as-a-tool-for-improving-sensing-performance-of-sensory-polymers
#13
Blanca S Pascual, Saúl Vallejos, Cipriano Ramos, María Teresa Sanz, José A Reglero Ruiz, Félix C García, José M García
Microcellular sensory polymers prepared from solid sensory polymeric films were tested in an aqueous Hg(II) detection process to analyze their sensory behavior. First, solid acrylic-based polymeric films of 100 µm thickness were obtained via radical copolymerization process. Secondly, dithizone sensoring motifs were anchored in a simple five-step route, obtaining handleable colorimetric sensory films. To create the microporous structure, films were foamed in a ScCO₂ batch process, carried out at 350 bar and 60 °C, resulting in homogeneous morphologies with cell sizes around 5 µm...
December 11, 2018: Sensors
https://www.readbyqxmd.com/read/30544942/determination-of-flavonoid-and-proanthocyanidin-profile-of-hungarian-sour-cherry
#14
Andrea Nemes, Erzsébet Szőllősi, László Stündl, Attila Biró, Judit Rita Homoki, Mária Magdolna Szarvas, Péter Balogh, Zoltán Cziáky, Judit Remenyik
Hungarian sour cherries (SC) are excellent source of anthocyanin (concentrations (100⁻300 mg in 100 g fresh fruit) and melatonin (0.15 mg in 100 g fresh fruit), but other flavonoid derivatives also can be isolated by aqueous alcoholic extraction. We have developed a new process for extracting non-extractable procyanidines bound to the membrane, proteins, and fibers. These compounds were seperated with UHPLC-MS methods, and the structure of individual components were identified on the basis of their mass fragmentation spectra...
December 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30544788/evaluation-of-the-potential-of-collagen-from-codfish-skin-as-a-biomaterial-for-biomedical-applications
#15
Ana M Carvalho, Alexandra P Marques, Tiago H Silva, Rui L Reis
Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines...
December 8, 2018: Marine Drugs
https://www.readbyqxmd.com/read/30544695/indicator-layers-based-on-ethylene-vinyl-acetate-copolymer-eva-and-dicyanovinyl-azobenzene-dyes-for-fast-and-selective-evaluation-of-vaporous-biogenic-amines
#16
Tinkara Mastnak, Aleksandra Lobnik, Gerhard J Mohr, Matjaž Finšgar
The article presents naked-eye methods for fast, sensitive, and selective detection of isopentylamine and cadaverine vapours based on 4- N , N -dioctylamino-4'-dicyanovinylazobenzene (CR-528) and 4- N , N -dioctylamino-2'-nitro-4'-dicyanovinylazobenzene (CR-555) dyes immobilized in ethylene-vinyl acetate copolymer (EVA). The reaction of CR-528/EVA and CR-555/EVA indicator layers with isopentylamine vapours caused a vivid colour change from pink/purple to yellow/orange-yellow. Additionally, CR-555/EVA showed colour changes upon exposure to cadaverine...
December 10, 2018: Sensors
https://www.readbyqxmd.com/read/30543186/quantitative-measurement-of-urinary-proteins-in-2018-advantages-disadvantages-limits
#17
Anne Hay-Lombardie, Nicolas Pallet, Edith Bigot-Corbel
Today, there is no reference method for the measurement of urinary proteins. The difficulties are that urine is a very complex biological fluid, and that there are a high intra-and inter-individual variability in the protein excretion rate. Progress has been made during the last thirty years, but high analytical variability persists among the colorimetric or turbidimetric methods used for urinary proteins measurement.
December 1, 2018: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/30542946/progress-in-antibiotic-susceptibility-tests-a-comparative-review-with-special-emphasis-on-microfluidic-methods
#18
REVIEW
Zeeshan A Khan, Mohd F Siddiqui, Seungkyung Park
Antibiotic susceptibility test (AST) is an umbrella term for techniques to determine the susceptibility of bacteria to antibiotics. The antibiotic-resistant bacteria are a major threat to public health and a directed therapy based on accurate AST results is paramount in resistance control. Here we have briefly covered the progress of conventional, molecular, and automated AST tools and their limitations. Various aspects of microfluidic AST such as optical, electrochemical, colorimetric, and mechanical methods have been critically reviewed...
December 12, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/30542813/a-highly-selective-and-sensitive-colorimetric-detection-of-uric-acid-in-human-serum-based-on-mos-2-catalyzed-oxidation-tmb
#19
Xue Wang, Qin Yao, Xiaomin Tang, Huiping Zhong, Ping Qiu, Xiaolei Wang
A simple, rapid, and effective colorimetric assay for the detection of uric acid (UA) has been built, using the MoS2 nanoflakes-catalyzed 3,3',5,5'-tetramethylbenzidine (TMB)-H2 O2 system. In the presence of oxygen and uricase, uric acid was oxidized specifically to produce H2 O2 . MoS2 nanoflakes synthesized by hydrothermal reaction could catalyze the oxidation of TMB by H2 O2 , and engendering the colorimetric signal. Finally, the change in the color from colorless to blue was seen with naked eye, indicating the presence or absence of UA...
December 12, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30539253/colorimetric-determination-of-glucose-in-solution-and-via-the-use-of-a-paper-strip-by-exploiting-the-peroxidase-and-oxidase-mimicking-activity-of-bimetallic-cu-pd-nanoparticles-deposited-on-reduced-graphene-oxide-graphitic-carbon-nitride-or-mos-2-nanosheets
#20
Gitashree Darabdhara, Purna K Boruah, Manash R Das
This work describes the preparation of bimetallic Cu-Pd nanoparticles (NPs) on supports like reduced graphene oxide (rGO), graphitic carbon nitride (g-C3 N4 ) and MoS2 sheets with a size of <10 nm. rGO is found to be the best support for synthesizing Cu-Pd NPs with controlled shape, size and oxidation state. The Cu-Pd/rGO nanocomposite also demonstrated the best peroxidase and oxidase mimicking activity compared to Cu-Pd/g-C3 N4 and Cu-Pd/MoS2 nanocomposites. The peroxidase mimicking activity of Cu-Pd/rGO was investigated in more detail, and a glucose oxidase (GOx) based glucose sensor was constructed that is based on the enzymatic formation of H2 O2 and the Cu-Pd NPs-assisted oxidation of tetramethylbenzidine by H2 O2 to give a blue-green coloration with absorption maxima at 652 nm...
December 11, 2018: Mikrochimica Acta
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