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colon stem cell

Marcelo D Goissis, Mariana I Giassetti, Robinson A Worst, Camilla M Mendes, Pedro V Moreira, Mayra E O A Assumpção, Jose A Visintin
Spermatogonial stem cells (SSC) have the potential to restore spermatogenesis when transplanted into testes depleted of germ cells. Due to this property, SSC could be used in breeding programs and in transgenic animal research. Particularly in cattle, SSC are not as well characterized as in mice or humans. In mice, C-X-C Motif Chemokine Receptor 4 positive (CXCR4+) testicular cells have high SSC potential. It, therefore, was hypothesized that CXCR4 is a marker of undifferentiated spermatogonia in cattle. Using samples from pre-pubertal calves, the CXCR4 protein was detected by immunohistochemistry in a few cells of the seminiferous tubules...
August 11, 2018: Animal Reproduction Science
Xiao Tan, Zhongqiang Zhang, Hongliang Yao, Liangfang Shen
T-cell immunoglobulin domain and mucin domain-4 (Tim-4) is overexpressed in several tumors and is correlated with enhanced tumor development and metastasis. In this study, we investigated the physiological alterations and molecular events related to Tim-4 overexpression in a mouse model of colorectal cancer (CRC). In the current study, we observed that Tim-4 is upregulated in CRC tissues compared with neighboring normal tissues. In addition, statistical analysis revealed that elevated Tim-4 expression was strongly linked to distant metastasis, TNM stage and reduced overall survival duration in CRC patients...
August 14, 2018: Cancer Letters
Ya-Ling Hsu, Yi-Jen Chen, Wei-An Chang, Shu-Fang Jian, Hsiao-Li Fan, Jaw-Yuan Wang, Po-Lin Kuo
Crosstalk of a tumor with its microenvironment is a critical factor contributing to cancer development. This study investigates the soluble factors released by tumor-associated dendritic cells (TADCs) responsible for increasing cancer stem cell (CSC) properties, cell mobility, and epithelial-to-mesenchymal transition (EMT). Dendritic cells (DCs) of colon cancer patients were collected for phenotype and CXCL1 expression by flow cytometry and Luminex assays. The transcriptome of CXCL1-treated cancer cells was established by next generation sequencing...
August 16, 2018: International Journal of Molecular Sciences
Guillemette Fouquet, Tereza Coman, Olivier Hermine, Francine Côté
A large number of studies have focused on the role of serotonin as a neurotransmitter in the central nervous system, although only a small percentage of the body's serotonin (∼5%) can be found in the mature brain of mammals. In the gut, the enterochromaffin cells are scattered in the enteric epithelium from the stomach through the colon and produce over 95% of the body's serotonin. Since the generation of tryptophan hydroxylase (Tph1 and Tph2) knockout mice, unsuspected roles have been identified for serotonin synthesized outside the brain...
August 11, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Dongchang Zhao, Yeung-Hyen Kim, Seihwan Jeong, Joel K Greenson, Mohammed S Chaudhry, Matthias Hoepting, Erik R Anderson, Marcel Rm van den Brink, Jonathan U Peled, Antonio Lc Gomes, Ann E Slingerland, Michael J Donovan, Andrew C Harris, John E Levine, Umut Özbek, Lora V Hooper, Thaddeus S Stappenbeck, Aaron M Ver Heul, Ta-Chiang Liu, Pavan Reddy, James Lm Ferrara
Graft-versus-host disease (GVHD) in the gastrointestinal (GI) tract remains the major cause of morbidity and non-relapse mortality after bone marrow transplantation (BMT). The Paneth cell protein, regenerating islet-derived 3-alpha (REG3α), is a biomarker specific for GI GVHD. REG3α serum levels rose in the systematic circulation as GVHD progressively destroyed Paneth cells and reduced GI epithelial barrier function. Paradoxically, GVHD suppressed intestinal REG3γ (the mouse homologue of human REG3α), and the absence of REG3γ in BMT recipients intensified GVHD but did not change the composition of the microbiome...
August 14, 2018: Journal of Clinical Investigation
Guo Chao Niu, Lei Liu, Libo Zheng, Hong Zhang, David Q Shih, Xiaolan Zhang
BACKGROUND: A variety of extra-intestinal manifestations (EIMs), including hepatobiliary complications, are associated with inflammatory bowel disease (IBD). Mesenchymal stem cells (MSCs) have been shown to play a potential role in the therapy of IBD. This study was designed to investigate the effect and mechanism of MSCs on chronic colitis-associated hepatobiliary complications using mouse chronic colitis models induced by dextran sulfate sodium (DSS). METHODS: DSS-induced mouse chronic colitis models were established and treated with MSCs...
August 13, 2018: BMC Gastroenterology
Oluwaseun Adebayo Bamodu, Ching-Kuo Yang, Wei-Hong Cheng, David T W Tzeng, Kuang-Tai Kuo, Chun-Chih Huang, Li Deng, Michael Hsiao, Wei-Hwa Lee, Chi-Tai Yeh
BACKGROUND: Colorectal cancer (CRC) remains a leading cause of cancer-related morbidity and mortality in both sexes globally. This is not unconnected with the heterogeneity and plasticity of CRC stem cells (CRC-SCs) which stealthily exploit the niche-related and (epi)genetic factors to facilitate metastasis, chemoresistance, tumor recurrence, and disease progression. Despite the accumulating evidence of the role of dysregulated microRNAs in malignancies, the therapeutic efficacy of pharmacological-targeting of CRC-SC-associated microRNAs is relatively under-explored...
August 10, 2018: Cancers
Qiliang Zhou, Xulu Ye, Qingsong Ran, Akihiko Kitahara, Yoshifumi Matsumoto, Masato Moriyama, Yoichi Ajioka, Yasuo Saijo
The outcomes of tracheal transplantation for the treatment of airway stenosis are unsatisfactory. We investigated the feasibility of regeneration of the trachea using a rat decellularized tracheal scaffold and mouse induced pluripotent stem (iPS) cells for in vivo transplantation. The rat trachea was first decellularized using a detergent-enzymatic treatment method. We successfully established a centrifugation method that can transplant cells onto the luminal surface of the decellularized rat tracheal scaffold circumferentially...
August 13, 2018: Tissue Engineering. Part C, Methods
Christina K Cajigas-Du Ross, Shannalee R Martinez, Leanne Woods-Burnham, Alfonso M Durán, Sourav Roy, Anamika Basu, Joshua A Ramirez, Greisha L Ortiz-Hernández, Leslimar Ríos-Colón, Evgeny Chirshev, Evelyn S Sanchez-Hernandez, Ubaldo Soto, Celine Greco, Claude Boucheix, Xin Chen, Juli Unternaehrer, Charles Wang, Carlos A Casiano
Patients with metastatic castration-resistant prostate cancer (mCRPC) develop resistance to conventional therapies including docetaxel (DTX). Identifying molecular pathways underlying DTX resistance is critical for developing novel combinatorial therapies to prevent or reverse this resistance. To identify transcriptomic signatures associated with acquisition of chemoresistance we profiled gene expression in DTX-sensitive and -resistant mCRPC cells using RNA sequencing (RNA-seq). PC3 and DU145 cells were selected for DTX resistance and this phenotype was validated by immunoblotting using DTX resistance markers (e...
July 13, 2018: Oncotarget
Jun Hee Lee, Chul Won Yun, Yong-Seok Han, SangMin Kim, Dongjun Jeong, Hyog Young Kwon, Hyeongjoo Kim, Moo-Jun Baek, Sang Hun Lee
Melatonin suppresses tumor development. However, the exact relationship between melatonin and cancer stem cells (CSCs) is poorly understood. This study found that melatonin inhibits colon CSCs by regulating the PrPC -Oct4 axis. In specimens from patients with colorectal cancer, the expressions of cellular prion protein (PrPC ) and Oct4 were significantly correlated with metastasis and tumor stages. Co-treatment with 5-fluorouracil (5-FU) and melatonin inhibited the stem cell markers Oct4, Nanog, Sox2, and ALDH1A1 by downregulating PrPC ...
August 9, 2018: Journal of Pineal Research
Jing Ren, Liang Ding, Dongya Zhang, Guoping Shi, Qianyun Xu, Sunan Shen, Yaping Wang, Tingting Wang, Yayi Hou
Long non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). The crosstalk between carcinoma- associated fibroblasts (CAFs) and cancer cells in the tumor microenvironment promotes tumor development and confers chemoresistance. In this study, we further investigated the underlying tumor-promoting roles of CAFs and the molecular mediators involved in these processes. Methods: The AOM/DSS-induced colitis-associated cancer (CAC) mouse model was established, and RNA sequencing was performed...
2018: Theranostics
Hwa Jin Kim, Sue Jin Moon, Seok-Hyung Kim, Kyu Heo, Jeong Hoon Kim
Metastasis-associated in colon cancer 1 (MACC1) has been reported to be overexpressed in multiple cancers and promote proliferation, metastasis, cancer stem cell-like properties, and drug resistance of cancer cells. Despite its significance and the considerable knowledge accumulated on the function of MACC1 in various types of human malignancies, regulatory mechanisms underlying MACC1 expression remain unclear. Here we report that MACC1 is a direct target of Wnt/β-catenin signaling pathway in colon cancer cells and that DBC1 functions as a coactivator for Wnt-mediated MACC1 expression by promoting the activity of a LEF1/β-catenin-dependent enhancer located in intron 1 of MACC1 gene...
August 6, 2018: Cell Death & Disease
Xiyue Chen, Peixia Song, Peixin Fan, Ting He, Devin Jacobs, Crystal L Levesque, Lee J Johnston, Linbao Ji, Ning Ma, Yiqiang Chen, Jie Zhang, Jinshan Zhao, Xi Ma
Appropriate protein concentration is essential for animal at certain stage. This study evaluated the effects of different percentages of dietary protein restriction on intestinal health of growing pigs. Eighteen barrows were randomly assigned to a normal (18%), low (15%), and extremely low (12%) dietary protein concentration group for 30 days. Intestinal morphology and permeability, bacterial communities, expressions, and distributions of intestinal tight junction proteins, expressions of biomarkers of intestinal stem cells (ISCs) and chymous bacterial metabolites in ileum and colon were detected...
2018: Frontiers in Cellular and Infection Microbiology
Yusuke Kanda, Tokuichi Kawaguchi, Mitsuhiko Osaki, Kunishige Onuma, Takahiro Ochiya, Tomoyuki Kitagawa, Futoshi Okada
OBJECTIVE: In sporadic colon tumors, multistep process of well-known genetic alterations accelerates carcinogenesis; however, this does not appear to be the case in inflammation-related ones. We previously established a model of inflammation-related colon carcinogenesis using human colonic adenoma cells, and identified fascin as a driver gene of this process. We analyzed the microRNAs involved in the stable fascin expression in colon adenocarcinoma cells. MATERIALS AND METHODS: miRNA microarray analysis was performed using FPCK-1-1 adenoma cells and its-derived FPCKpP1 -4 adenocarcinoma cells through chronic inflammation...
July 28, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Peng Chen, Toshihiro Aso, Ryuichiro Sasaki, Maki Ashida, Yusuke Tsutsumi, Hisashi Doi, Takao Hanawa
To clarify the effects of grid topographies with different scales on cell morphology and functionalization, we investigated the adhesion and differentiation of human mesenchymal stem cells (hMSCs) to titanium surfaces with micron, nano, and micron/nano (hybrid) grid topographies created by femtosecond laser irradiation. The results showed that cellular adhesion and differentiation strongly depended on the scales of the grid topography. hMSCs cultured on micron and hybrid grid topographies showed regulation of cellular adhesion plaques following the surface topography and were vinculin-positive, whereas filamentous vinculin was evident at the filopodia of hMSCs cultured on nanogrids...
July 28, 2018: Journal of Biomedical Materials Research. Part A
Hamid Tanzadehpanah, Hanie Mahaki, Pouria Samadi, Jamshid Karimi, Neda Hosseinpour Moghadam, Sadegh Salehzadeh, Dara Dastan, Massoud Saidijam
In this study, Farnesiferol C was introduced as an anti-colon cancer agent. Its cytotoxicity was investigated on two cancer cell lines, HCT116 and CT26, and mesenchymal stem cells (MSCs) as normal cells employing MTT assay. Moreover, Farnesiferol C interactions with ct-DNA and HSA were investigated by various techniques. The IC50 values of Farnesiferol C on HCT116 and CT26 cells were 42 and 46 µM, respectively, while its IC50 value on MSCs cells was 92 µM, indicating that Farnesiferol C was more efficacious against cancer cell lines than normal cells...
July 27, 2018: Journal of Biomolecular Structure & Dynamics
Sebastian Scheich, Sarah Weber, Rosalie König, Anne C Wilke, Sarah Lindner, Claudia Reinheimer, Thomas A Wichelhaus, Michael Hogardt, Volkhard A J Kempf, Johanna Kessel, Hans Martin, Gesine Bug, Hubert Serve, Björn Steffen
BACKGROUND: In haematology and oncology, in particular in the setting of allogeneic hematopoietic stem cell transplantation (allo-HSCT), VRE colonization rates are high due to previous hospital stays and preceding antibiotic treatment and colonized patients have a lower overall survival (OS). OBJECTIVE: We reanalysed our previously published cohort, to unravel which colonization timepoints before and during allo-HSCT might be predictive for the subsequent outcome...
July 26, 2018: European Journal of Haematology
Tilahun Ayane Debele, Lu-Yi Yu, Cheng-Sheng Yang, Yao-An Shen, Chun-Liang Lo
A dual-sensitive polymeric drug conjugate (HA-SS-MP) was synthesized by conjugating hydrophobic 6-mercaptopurine (MP) to thiolated hyaluronic acid (HA) as the carrier and ligand to deliver doxorubicin (Dox) to parental colon cancer and colon cancer stem cells. Because of the amphiphilic nature of HA-SS-MP, it was self-assembled in the aqueous media, and Dox was physically encapsulated in the core of the micelles. The particle size and the zeta potential of the micelle were analyzed by dynamic light scattering (DLS), and the morphology of the micelle was investigated using transmission electron microscopy (TEM)...
August 9, 2018: Biomacromolecules
Tomoyuki Nakajima, Takeshi Uehara, Yukihiro Kobayashi, Yasuhiro Kinugawa, Kazuhiro Yamanoi, Yasuhiro Maruyama, Tomoaki Suga, Hiroyoshi Ota
LGR5 is expressed in various tumors and has been identified as a putative intestinal stem cell marker. Here we investigated LGR5 expression in colorectal neuroendocrine neoplasms and analyzed the correlation with pathological characteristics. We evaluated the clinicopathological features of 8 neuroendocrine tumor (NET) grade 1 (NET G1), 4 NET Grade 2 (NET G2), and 8 NET Grade 3 (NET G3; also termed neuroendocrine carcinoma, or NEC) cases. We examined LGR5 expression using an RNAscope, a newly developed RNA in situ hybridization technique, with a tissue microarray of the neuroendocrine neoplasm samples...
July 24, 2018: Pathology International
Yukiko Miyatake, Yusuke Ohta, Shunji Ikeshita, Masanori Kasahara
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal refractory cancers. Aggressive features in PDAC cells have been well studied, but those exhibited by a population of PDAC cells are largely unknown. We show here that coculture with epithelial-like feeder cells confers more malignant phenotypes upon PDAC cells forming anchorage-dependent multicellular aggregates (Ad-MCAs, a behavior of collective cells), in vitro . When CD44v3-10high /CD44slow PDAC cell lines, which exhibited an epithelial phenotype before the onset of epithelial-mesenchymal transition (EMT), were cocultured with a monolayer of HEK293T cells overnight, they formed Ad-MCAs on the feeder layer and acquired gemcitabine resistance...
July 6, 2018: Oncotarget
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