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Diagnostic markers

Tatsuo Hata, Masamichi Mizuma, Fuyuhiko Motoi, Masaharu Ishida, Takanori Morikawa, Tatsuyuki Takadate, Kei Nakagawa, Hiroki Hayashi, Atsushi Kanno, Atsushi Masamune, Takashi Kamei, Toru Furukawa, Takeshi Naitoh, Michiaki Unno
OBJECTIVES: The aim of this study was to evaluate the diagnostic and prognostic impact of systemic inflammatory markers for IPMN with high-grade dysplasia (HGD)/invasive carcinoma. METHODS: Neutrophil-to-lymphocyte ratio (NLR), derived NLR, platelet-to-lymphocyte ratio, and C-reactive protein-to-albumin ratio were compared across the different histological grades of 205 IPMN cases. We also tested the diagnostic performance for IPMN with HGD/invasive carcinoma. RESULTS: The median (interquartile range) preoperative NLR was higher in IPMN with HGD/invasive carcinoma (2...
January 2019: Pancreas
Clémentine Sarkozy, Christiane Copie-Bergmand, Diane Damotte, Susana Ben-Neriah, Barbara Burroni, Jérome Cornillon, Richard Lemal, Camille Golfier, Bettina Fabiani, Catherine Chassagne-Clément, Marie Parrens, Charles Herbaux, Luc Xerri, Celine Bossard, Camille Laurent, Morgane Cheminant, Guillaume Cartron, Jose Cabecadas, Thierry Molina, Gilles Salles, Christian Steidl, Hervé Ghesquières, Anja Mottok, Alexandra Traverse-Glehen
Gray-zone lymphoma (GZL) with features intermediate between classic Hodgkin lymphoma (cHL) and large B-cell lymphoma (LBCL) was introduced as a provisional entity into the World Health Organization classification in 2008. However, as diagnostic criteria are imprecise, reliable identification of GZL cases remains challenging. Here, we describe the histopathologic features of 139 GZL cases from a retrospective Lymphoma Study Association (LYSA) study with the goal to improve classification accuracy. Inclusion criteria were based on literature review and an expert consensus opinion of the LYSA hematopathologist panel...
December 11, 2018: American Journal of Surgical Pathology
Monica Maia de Oliveira Barros, Karla Regina Oliveira de Moura Ronchini, Rosa Leonôra Salerno Soares
BACKGROUND: Hepatitis B and C are diseases with high morbimortality and constitute a global public health problem. In Brazil, the prevalence is not homogeneous, oscillating among different regions, but it is estimated that currently about 1% of the population present chronic disease related to the B virus and that there are 1.5 million infected with the C virus. Despite the development of hepatitis B vaccine, improvement in diagnostic methods and therapeutic advances in the field of viral hepatitis, there is still a large number of people who continues to be infected by these viruses, especially in populations at risk and also due to several factors, including vaccination and migration policies...
July 2018: Arquivos de Gastroenterologia
Julie-Myrtille Bourgognon, Joern R Steinert
Neurodegenerative disorders are often associated with cellular dysfunction caused by underlying protein-misfolding signalling. Numerous neuropathologies are diagnosed at late stage symptomatic changes which occur in response to these molecular malfunctions and treatment is often too late or restricted only to the slowing of further cell death. Important new strategies to identify early biomarkers with predictive value to intervene with disease progression at stages where cell dysfunction has not progressed irreversibly is of paramount importance...
March 2019: Neural Regeneration Research
Anci Verlemyr, Lennart Truedsson, Lillemor Skattum
Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions...
2019: Methods in Molecular Biology
Shino Magaki, Seyed A Hojat, Bowen Wei, Alexandra So, William H Yong
Immunohistochemistry (IHC) is a powerful technique that exploits the specific binding between an antibody and antigen to detect and localize specific antigens in cells and tissue, most commonly detected and examined with the light microscope. A standard tool in many fields in the research setting, IHC has become an essential ancillary technique in clinical diagnostics in anatomic pathology (Lin F, Chen Z. Arch Pathol Lab Med 138:1564-1577, 2014) with the advent of antigen retrieval methods allowing it to be performed conveniently on formalin fixed paraffin embedded (FFPE) tissue (Taylor CR, Shi S-R, Barr NJ...
2019: Methods in Molecular Biology
Thurid Ahlenstiel-Grunow, Lars Pape
After pediatric kidney transplantation BK polyomavirus (BKPyV) infections are associated with an increased risk of graft loss by BKPyV-associated nephropathy (BkPyVAN). However, suitable prognostic markers for the individual outcome of BKPyV infections are missing and the management of therapeutic interventions remains a challenge to the success of pediatric kidney transplantation. This review gives an overview on current diagnostic and therapeutic strategies in the field of BKPyV infections after pediatric kidney transplantation...
December 11, 2018: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
Ju-Yi Li, Biao Cheng, Xiu-Fang Wang, Zhong-Jing Wang, Hong-Mei Zhang, Shu-Fen Liu, Li-Sha Chen, Wen-Jing Huang, Jue Liu, Ai-Ping Deng
Critical limb ischemia (CLI) is the most severe manifestation of peripheral artery disease, which is common but rarely diagnosed. Noninvasive biomarkers are urgently required to assist in the diagnosis of CLI. Accumulating evidence indicates that miRNAs play an important role in the development of various diseases. In this study, microarray profiling revealed 11 miRNAs with significantly altered expression in four T2DM patients with CLI compared with that in four sex- and age-matched T2DM patients without CLI...
2018: BioMed Research International
Saikou Y Bah, Collins Misita Morang'a, Jonas A Kengne-Ouafo, Lucas Amenga-Etego, Gordon A Awandare
Genomics and bioinformatics are increasingly contributing to our understanding of infectious diseases caused by bacterial pathogens such as Mycobacterium tuberculosis and parasites such as Plasmodium falciparum . This ranges from investigations of disease outbreaks and pathogenesis, host and pathogen genomic variation, and host immune evasion mechanisms to identification of potential diagnostic markers and vaccine targets. High throughput genomics data generated from pathogens and animal models can be combined with host genomics and patients' health records to give advice on treatment options as well as potential drug and vaccine interactions...
2018: Frontiers in Genetics
Luis Carlos Nacul, Kathleen Mudie, Caroline C Kingdon, Taane G Clark, Eliana Mattos Lacerda
Background: The diagnosis of myalgic encephalomyelitis (ME/CFS) in research and clinical practice has largely relied on clinical history, which can be subjective in nature. Clinical signs are often subtle, overlap with other conditions, and are not formally included as part of diagnostic workup. The characterization of clinical signs and biomarkers is needed for better diagnosis and classification of patients and to monitor treatment response. Hand grip strength (HGS) has been used as an objective measure of muscle strength and fatigue, which is a primary symptom of ME/CFS...
2018: Frontiers in Neurology
Wenjie Zheng, Junling Yang, Zhizhen Dong, Li Wang, Miao Fang, Wei Wu, Dengfu Yao, Min Yao
Purpose: High mobility group box 3 (HMGB3) is associated with hepatocytes malignant transformation by our previous work. We continued to investigate the diagnostic and prognostic values of HMGB3 for hepatocellular carcinoma (HCC). Patients and methods: Circulating HMGB3 levels were quantitatively detected in a cohort of 225 patients with chronic liver diseases by ELISA and compared with alpha-fetoprotein by the receiver operating characteristic curve. HMGB3 expression in tissues of 170 HCC was detected by tissue microarray and immunohistochemistry...
2018: Cancer Management and Research
Anas A Youssef, Hisham A Issa, Maha Z Omar, Eman G Behiry, Asmaa A Elfallah, Ali Hasaneen, Mohamed Darwish, Dalia B Ibrahim
Objectives: The aim of this study was to assess serum levels of endocan & VEGF in patients with hepatitis C virus-related HCC and their diagnostic and predictive value of mortality. Methods: A total of 195 patients with CHC were subdivided into the following two groups: 105 HCV cirrhotic patients with HCC and 90 HCV cirrhotic patients without HCC. Sixty apparently healthy subjects served as the control group. The serum VEGF and endocan were assessed by ELISA...
2018: Clinical and Experimental Gastroenterology
Kitti Intuyod, Paula Saavedra-García, Stefania Zona, Chun-Fui Lai, Yannasittha Jiramongkol, Kulthida Vaeteewoottacharn, Chawalit Pairojkul, Shang Yao, Jay-Sze Yong, Sasanan Trakansuebkul, Sakda Waraasawapati, Vor Luvira, Sopit Wongkham, Somchai Pinlaor, Eric W-F Lam
Fluorouracil (5-FU) is the first-line chemotherapeutic drug for cholangiocarcinoma (CCA), but its efficacy has been compromised by the development of resistance. Development of 5-FU resistance is associated with elevated expression of its cellular target, thymidylate synthase (TYMS). E2F1 transcription factor has previously been shown to modulate the expression of FOXM1 and TYMS. Immunohistochemical (IHC) analysis revealed a strong correlated upregulation of FOXM1 (78%) and TYMS (48%) expression at the protein levels in CCA tissues...
December 11, 2018: Cell Death & Disease
Paulina Krawiec, Elżbieta Pac-Kożuchowska
BACKGROUND: There is no single reliable marker of iron homeostasis in inflammatory bowel disease. AIMS: To determine diagnostic usefulness of soluble transferrin receptor and soluble transferrin receptor/log ferritin index in iron deficiency anemia in children with inflammatory bowel disease. METHODS: We assessed soluble transferrin receptor in serum and calculated soluble transferrin receptor/log ferritin index in 75 children with inflammatory bowel disease...
November 22, 2018: Digestive and Liver Disease
Karel Lábr, Jindřich Špinar, Jiří Pařenica, Lenka Špinarová, Filip Málek, Monika Špinarová, Ondřej Ludka, Jiří Jarkovský, Klára Benešová, Monika Goldbergová-Pávková, Růžena Lábrová
BACKGROUND/AIMS: The rate of incidence and prevalence of acute kidney injury is increasing due to an increased number of patients with heart failure. Therefore it is very pertinent to early detect the level of renal injuries and to make necessary heart failure predictions. Thus the aim of this study is to determine renal functions and prognosis stratification in chronic heart failure patients and importance of Neutrophil Gelatinase-Associated Lipocalin (NGAL), an early diagnostic marker of acute kidney injury, as well as stratification of cardiovascular risk in heart failure patients...
December 7, 2018: Kidney & Blood Pressure Research
Nancy Charo, Emilia Scharrig, María F Ferrer, Norberto San Juan, Eugenio A Carrera Silva, Mirta Schattner, Ricardo M Gómez
Leptospirosis is a global zoonosis caused by pathogenic Leptospira. Neutrophils are key cells against bacterial pathogens but can also contribute to tissue damage. Since the information regarding the role of human neutrophils in leptospirosis is scant, we comparatively analyzed the human neutrophil's response to saprophytic Leptospira biflexa serovar Patoc (Patoc) and the pathogenic Leptospira interrogans serovar Copenhageni (LIC). Both species triggered neutrophil responses involved in migration, including the up-regulation of CD11b expression, adhesion to collagen and the release of IL-8...
December 10, 2018: Cellular Microbiology
Yuan Liao, Jiao Gong, Wenying Zhou, Huimin Dong, Jiayin Liang, Minqi Luo, Bo Hu
Chronic liver inflammation caused by chronic hepatitis B virus (CHB) infection leads to liver cirrhosis and hepatocellular carcinoma. Recently, the role of alanine aminotransferase (ALT) as a predictor of liver inflammation has been questioned. The aim of this study was to investigate the utility of noninvasive fibrosis markers including hyaluronic acid (HA), collagen type IV (CIV), N-terminal propeptide of type III procollagen (PIIINP), and laminin (LN) in identifying significant liver inflammation in patients with CHB, especially in patients with normal or near-normal ALT...
December 10, 2018: Journal of Medical Virology
Nina Homayoon, Lukas Pirpamer, Sebastian Franthal, Petra Katschnig-Winter, Mariella Kögl, Stephan Seiler, Karoline Wenzel, Edith Hofer, Hannes Deutschmann, Franz Fazekas, Christian Langkammer, Stefan Ropele, Reinhold Schmidt, Petra Schwingenschuh
OBJECTIVE: We investigated R2* relaxation rates as a marker of iron content in the substantia nigra in patients with common tremor disorders and explored their diagnostic properties. METHODS: Mean nigral R2* rates were measured in 40 patients with tremor-dominant Parkinson's disease (PD), 15 with tremor in dystonia, 25 with essential tremor, and 25 healthy controls. RESULTS: Tremor-dominant PD patients had significantly higher nigral R2* values (34...
December 10, 2018: Movement Disorders: Official Journal of the Movement Disorder Society
Kavitha Prasad, Roopa Rao, Dominic Augustine, Samudrala Venkatesiah Sowmya, Vanishri Haragannavar, Parimala Sagar, Prathibha Sreedhar
BACKGROUND: The objective was to study comprehensive mRNA expression profiles of buccal mucosa oral squamous cell carcinoma (OSCC-BM) and gingivo-buccal OSCC (OSCC-GB) in smokeless tobacco chewers to understand the biological behavior of OSCC at these specific sites and identify diagnostic and prognostic markers. METHODS: High throughput RNA sequencing transcriptome of fresh buccal mucosa (4 samples) and gingivo-buccal (4 samples) OSCC with normal oral mucosa (3 samples) was performed on Illumina NextSeq500 paired end sequencing with 75x2bp...
December 11, 2018: Head & Neck
Wen-Bin Liu, Fei Han, Yong-Sheng Huang, Hong-Qiang Chen, Jian-Ping Chen, Dan-Dan Wang, Xiao Jiang, Li Yin, Jia Cao, Jin-Yi Liu
Emerging evidences have revealed tumor-specific gene methylation is considered to be a promising non-invasive biomarker for many different types of cancers. This study was determined whether TMEM196 gene hypermethylation and downregulation are considered to be promising biomarkers for early diagnosis and prognosis in lung cancer. Methylation status was detected with methylation-specific PCR. Kaplan-Meier survival curves and Cox regression analysis were used to determine the significance of prognosis. TMEM196 gene was hypermethylated in 68...
December 11, 2018: Molecular Carcinogenesis
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