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Fibroblast collagen

Jing Zong, Hao Zhang, Fang-Fang Li, Kai Liang, Jia-Li Liu, Lu-Hong Xu, Wen-Hao Qian
Nuclear localization leucine-rich-repeat protein 1 (NLRP1) is a member of Nod-like receptors (NLRs) family. Recent studies have reported that NLRP1 is involved in various diseases, especially in cardiovascular diseases. However, the effect of NLRP1 on cardiac fibrosis remains unclear. In this study, NLRP1 overexpression and NLRP1 silencing constructs were transfected into neonatal rat cardiac fibroblasts induced by TGF-β1 for 48 h to investigate the effect of NLRP1 in cardiac fibrosis and its molecular mechanisms...
August 18, 2018: Journal of Molecular Histology
Jia Tao, Min Zhang, Zhijie Wen, Baoxue Wang, Lei Zhang, Yu Ou, Xu Tang, Xiaoping Yu, Qinglin Jiang
OBJECTIVES: Pulmonary fibrosis is strongly correlated with inflammation factors, cytokine, and collagen secretion, whereby discoidin domain receptor 1 (DDR1) signaling plays an important role. EP300 is defined as an acetyltransferase that can acetylate histone and has been broadly studied in several chronic diseases, including cancer, inflammation and fibrosis. This study aimed to investigate the relationship between p300 and DDR1 in the pathological processes of pulmonary fibrosis. MATERIALS AND METHODS: Transcriptome analysis of single cell RNA-sequencing for idiopathic pulmonary fibrosis (IPF) bronchial epithelial cells demonstrated that both DDR1 and EP300 were up-regulated and involved in the regulation of autophagy, cellular response to organonitrogen compounds, and collagen metabolic pathways, respectively...
July 30, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Shanlong Huang, Chuance Yang, Min Li, Binxian Wang, Hongwei Chen, Delai Fu, Tie Chong
BACKGROUND: TGFβ1 and mTOR are considered to play important roles in fibrotic diseases. Rapamycin has been reported to inhibit urethral stricture formation in a rabbit model of urethral fibrosis. AIM: To evaluate if dual mTOR inhibitor has a superior efficacy compared with rapamycin on inhibiting cell proliferation and collagen expression in human urethral scar fibroblasts (HUSFs). METHODS: We established HUSF cultures from fresh surgical specimen...
July 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Chien-Chi Lin, Murray Korc
Pancreatic ductal adenocarcinoma (PDAC) is currently the third leading cause of cancer mortality in the United States, with a 5-year survival of ∼8%. PDAC is characterized by a dense and hypo-vascularized stroma consisting of proliferating cancer cells, cancer-associated fibroblasts, macrophages and immune cells, as well as excess matrices including collagens, fibronectin, and hyaluronic acid. In addition, PDAC has increased interstitial pressures and a hypoxic/acidic tumor microenvironment (TME) that impedes drug delivery and blocks cancer-directed immune mechanisms...
August 14, 2018: Cancer Letters
Seong-Wook Seo, Seul-Ki Park, Soo-Jin Oh, Ok Sarah Shin
Exposure to ultraviolet (UV) radiation is a major contributing factor to premature aging (photoaging) and skin cancer. In vitro models of cellular senescence have proven to be very useful for the study of slow and progressive accumulation of damage resulting in the growth arrest of aging skin cells. In this study, we compared UVA-induced cellular responses in non-senescent (NS) vs. senescent (S) human dermal fibroblasts (HDFs). HDFs were irradiated with a single dose of UVA (7.5 J/cm2) and QuantSeq 3' mRNA sequencing was performed to assess differential gene expression...
2018: PloS One
Chang-Jie Xiao, Xi-Jiao Yu, Jian-Li Xie, Shuang Liu, Shu Li
BACKGROUND: Curcumin exhibits anti-inflammatory effects and has been suggested as a treatment for inflammatory diseases. The aim of this study was to investigate the effects of curcumin on the lipopolysaccharide induced inflammatory response in rat gingival fibroblasts in vitro and ligation-induced experimental periodontitis in vivo, and to speculate the possible anti-inflammatory mechanism of curcumin. METHODS: The gingival fibroblasts were incubated with different concentrations of curcumin in the absence or presence of lipopolysaccharide (LPS)...
August 16, 2018: Head & Face Medicine
Wei-Wei Hao, Su-Li Zhang, Yan Sun, Li-Na Bai, Jing-Wei Bian, Hai-Cun Yu, Xiao-Chen Yin, Peng-Li Wang, Hui-Rong Liu
Myocardial fibrosis (MF) is an important pathological process of cardiac remodeling in patients with heart failure; however its etiology has not been clear. It has been known that the angiotensin II type 1 receptor autoantibody (AT1-AA) is present in patients with heart failure, but it is unclear whether this antibody directly causes MF. In this study, we investigated the role of AT1-AA in MF and its effects on cardiac fibroblasts (CFs). The AT1-AA positive rat model was established by active immunization method, and the measurement of indexes were made in the 8th week after active immunity...
August 25, 2018: Sheng Li Xue Bao: [Acta Physiologica Sinica]
Matthew J Cecchini, Karishma Hosein, Christopher J Howlett, Mariamma Joseph, Marco Mura
BACKGROUND: The clinical-radiographic distinction between idiopathic pulmonary fibrosis (IPF) and non-specific interstitial pneumonia (NSIP) is challenging. We sought to investigate the gene expression profiles of IPF and NSIP vs. normal controls. METHODS: Gene expression from explanted lungs of patients with IPF (n = 22), NSIP (n = 10) and from normal controls (n = 11) was assessed. Microarray analysis included Significance Analysis of Microarray (SAM), Ingenuity Pathway, Gene-Set Enrichment and unsupervised hierarchical clustering analyses...
August 15, 2018: Respiratory Research
Sei Won Lee, Lokesh Sharma, Young Ae Kang, Sang-Hun Kim, Sreelakshmi Chandrasekharan, Ashley Losier, Virginia Brady, Santos Bermejo, Nathaniel Andrews, Chang-Min Yoon, Wei Liu, Jung-Yeon Lee, Min-Jong Kang, Charles S Dela Cruz
Influenza viruses can result in significant lung injury with significant morbidity and mortality. In this study, we evaluated the impact of cigarette smoke (CS) exposure on the pulmonary fibroblastic response after influenza infection. We employed a murine model in which animals were exposed to cigarette smoke or room air and subsequently infected with H1N1 influenza virus. Inflammatory and fibrotic responses were measured at different time points post influenza infection. Primary fibroblasts were isolated from lung of mice and their characteristics were evaluated...
August 15, 2018: American Journal of Respiratory Cell and Molecular Biology
Won Il Choi, Youngmin Hwang, Abhishek Sahu, Kiyoon Min, Daekyung Sung, Giyoong Tae, Jeong Ho Chang
The use of injectable materials as a biofiller for soft tissue augmentation has been increasing worldwide. Levan is a biocompatible and inexpensive polysaccharide with great potential in biomaterial applications, but it has not been extensively studied. In this study, we evaluated the potential of levan as a new material for dermal fillers and prepared an injectable and physical levan-based hydrogel by combining levan with Pluronic and carboxymethyl cellulose (CMC). A sol state was prepared by mixing the polymers in a specific ratio at 4 °C for 2 days and the hydrogel was formed by increasing the temperature to 37 °C...
August 15, 2018: Biomaterials Science
Thayná Melo de Lima Morais, Patrícia Froes Meyer, Liliane Santos de Vasconcellos, Julio Costa E Silva, Ito Ferreira E Andrade, Vinicius Alves Freire de Farias, Igor Candido da Silva, Roberta Marinho Falcão Gondim Araújo, Rodrigo Marcel Valentim da Silva, Esteban Fortuny Pacheco, Ciro Dantas Soares
Extracorporeal shock wave therapy (ESWT) has been extensively studied for its multiple biological properties, and although it is widely applied in esthetical procedures, little is known about its effects on the epidermis and dermis. In this study, a histological and immunohistochemical study of the effects of ESWT was performed on rat skin. Forty-five female rats were treated with one or two sessions of ESWT and sacrificed on days 1, 7, 14, and 21 after treatment. The samples were histologically processed and then morphometric analyses were performed to assess the epidermis, dermis, and subcutaneous fat tissue thickness...
August 14, 2018: Lasers in Medical Science
Pei-Yuan Lee, Yen-Ching Liu, Mei-Xuan Wang, Jin-Jia Hu
The remodeling of fibroblast-seeded collagen gels in response to dynamic mechanical stimuli was investigated by using a newly developed biaxial culture system capable of cyclically stretching planar soft tissues. Fibroblast-seeded collagen gels were subjected to three distinct dynamic mechanical conditions for six days: Cyclic Equibiaxial Stretching at two constant strain magnitudes (CES-7% and CES-20%), and Cyclic Equibiaxial Stretching with incrementally Increasing stain magnitude (ICES, 7% → 15% → 20% each for two days)...
July 30, 2018: Journal of Biomechanics
X L Xu, J G Liu, M Sun, L Yu, Q Y Liu, Q M Bai, L J Wu, J Wang
Objective: To investigate the clinicopathologic and genetic features, pathologic diagnosis and differential diagnosis of angiofibroma of soft tissue(AFST). Methods: The clinicopathologic characteristics of 24 cases diagnosed at Fudan University Shanghai Cancer Center from 2011 to 2017 were analyzed; immunohistochemical staining and interphase fluorescence in situ hybridization (FISH) were performed, and the literatures were also reviewed. Results: There were 15 male and 9 female (male∶female=1.7∶1.0) patients with age of onset ranging from 8 to 68 years (mean, 45 years)...
August 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
Zhi-Yong Lei, Jing-Jing Chen, Zhi-Jian Cao, Ming-Zhang Ao, Long-Jiang Yu
ETHNOPHARMACOLOGICAL RELEVANCE: In traditional Chinese medicine, the aerial parts of Aeschynomene indica Linn. (AIL) have been used for wound healing, and to treat urinary tract infection, hepatitis, enteritis, dysentery, nyctalopia, conjunctivitis, urticaria, and furuncle. However, no scientific investigation has been conducted on its wound healing potential. AIM OF THE STUDY: To investigate the effects of AIL extract on wound healing, isolate the active constituent and reveal the possible mechanism of enhancing wound healing...
August 11, 2018: Journal of Ethnopharmacology
Tae-Rin Kwon, Chang Taek Oh, Dong-Ho Bak, Jong Hwan Kim, Joon Seok, Jong Hoon Lee, Su Hwan Lim, Kwang Ho Yoo, Beom Joon Kim, Heesu Kim
ETHNOPHARMACOLOGICAL RELEVANCE: Stichopus japonicus (sea cucumber), edible traditional food in Asia, and its extracts are renowned for their wound healing, pain relieving, and cosmetic effects in traditional medicine. Holothurins, toxins isolated from sea cucumber, are thought to be active components for their beneficial effects. However, researchers have yet to outline specific mechanisms thereof. AIM OF THE STUDY: The present study was designed to evaluate the anti-melanogenic and anti-wrinkle properties of S...
August 10, 2018: Journal of Ethnopharmacology
William H Trousdale, Christopher G Salib, Nicolas Reina, Eric A Lewallen, Anthony Viste, Daniel J Berry, Mark E Morrey, Joaquin Sanchez-Sotelo, Andre J van Wijnen, Matthew P Abdel
INTRODUCTION: The inflammatory cascade and production of prostaglandins may play a role in the pathogenesis of arthrofibrosis, a debilitating condition after joint replacement and other orthopedic procedures. Cyclooxygenase 2 (COX-2) inhibitors may mitigate the inflammatory response and formation of arthrofibrosis but oral delivery is associated with risk of systemic side effects in many patients. The non-steroidal anti-inflammatory drug (NSAID) drug celecoxib may have therapeutic benefits for arthrofibrosis, but current methods for its local delivery (e...
August 13, 2018: Tissue Engineering. Part C, Methods
Zun-En Xia, Juan-Li Xi, Lei Shi
3,3'-Diindolylmethane (DIM), a natural acid condensation extracted from cruciferous plants, exhibits anti-fibrotic effects in hepatic and cardiac fibrosis models. The effects of DIM on renal fibrosis, however, are unclear. This study aimed to explore the protective effects of DIM on renal fibrosis. Unilateral ureteral obstruction (UUO) and transforming growth factor (TGF)-β1-stimulated normal rat kidney (NRK)-49F fibroblast cell mouse models were established. The models were then treated with DIM for the assessment of its anti-fibrotic effects and mechanisms...
November 2018: Renal Failure
Amanda B Muir, Joshua X Wang, Hiroshi Nakagawa
Eosinophilic esophagitis (EoE) is a food allergen-induced inflammatory disorder. EoE is increasingly recognized as a cause of swallowing dysfunction, food impaction and esophageal stricture. Inflammation of the esophageal mucosa involves immune cell infiltrate, reactive epithelial changes and fibroblast activation, culminating in robust tissue remodeling toward esophageal fibrosis characterized by excess collagen deposition in the subepithelial lamina propria. Fibrosis contributes to a unique mechanical property of the EoE-affected esophagus that is substantially stiffer than the normal esophagus...
August 12, 2018: Journal of Gastroenterology
Maohua Shi, Jingnan Wang, Youjun Xiao, Cuicui Wang, Qian Qiu, Minxi Lao, Yangtao Yu, Zhifeng Li, Hongwei Zhang, Yujin Ye, Liuqin Liang, Xiuyan Yang, Guoqiang Chen, Hanshi Xu
Objective: To investigate the role of glycogen metabolism in regulating rheumatoid fibroblast-like synoviocyte (FLS)-mediated synovial inflammation and its underlying mechanism. Methods: FLSs were separated from synovial tissues (STs) obtained from rheumatoid arthritis (RA) patients. Glycogen content was determined by periodic acid Schiff staining. Protein expression was analyzed by Western blot or immunohistochemistry. Gene expression of cytokines and matrix metalloproteinases (MMPs) was evaluated by quantitative real-time PCR...
2018: Frontiers in Immunology
A K S Silva, T R N Silva, J R Nicoli, L M C Vasquez-Pinto, F S Martins
A healthy skin provides a protective barrier against pathogenic microorganisms. Recent studies have shown that probiotics, as those of Bifidobacterium genus, could act beneficially in dermatology, both when ingested and by topical use. In the present study, we evaluated by in vitro antagonism assays and using two skin cell lines the potential of four strains of Bifidobacterium spp. Among the four bifidobacteria, Bifidobacterium longum 51A was the only one able to inhibit the growth of the eight pathogenic indicators tested...
August 12, 2018: Letters in Applied Microbiology
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