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https://www.readbyqxmd.com/read/30516498/effectiveness-of-nonalcoholic-steatohepatitis-correction-on-the-ba%C3%B0-kground-of-obesity-with-concomitant-chronic-kidney-disease
#1
O Khukhlina, A Antoniv, L Kanovska, O Mandryk, V Smandych
The article presents a theoretical generalization of the research results the effectiveness of heparisin on the state of the carbohydrate-protein components of the extracellular matrix of connective tissue in liver in patients with non-alcoholic steatohepatitis with obesity I-II degree and chronic kidney disease І-ІІ stage. The purpose of the study is to find out the effectiveness of heparisin (glycyrizine 40 mg, glycine 400 mg, L-cysteine hydrochloride 20 mg) on the state of the carbohydrate-protein components of the extracellular matrix in connective tissue of the liver in patients with non-alcoholic steatohepatitis (NASH) with obesity I-II degree and chronic kidney disease (CKD) І-ІІ stage...
October 2018: Georgian Medical News
https://www.readbyqxmd.com/read/30513680/spacia1-saal1-deletion-results-in-a-moderate-delay-in-collagen-induced-arthritis-activity-along-with-mrna-decay-of-cyclin-dependent-kinase-6-gene
#2
Ryoji Fujii, Rie Komatsu, Tomoo Sato, Iwao Seki, Koji Konomi, Hiroyuki Aono, Hisateru Niki, Kazuo Yudoh, Kusuki Nishioka, Toshihiro Nakajima
This study was performed to elucidate the molecular function of the synoviocyte proliferation-associated in collagen-induced arthritis (CIA) 1/serum amyloid A-like 1 (SPACIA1/SAAL1) in mice CIA, an animal model of rheumatoid arthritis (RA), and human RA-synovial fibroblasts (RASFs). SPACIA1/SAAL1 -deficient mice were generated and used to create mouse models of CIA in mild or severe disease conditions. Cell cycle-related genes, whose expression levels were affected by SPACIA1/SAAL1 small interfering RNA (siRNA), were screened...
November 30, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30511978/assisting-rapid-soft-tissue-expansion-with-adipose-derived-stem-cells-an-experimental-study-in-a-pig-model
#3
Qianwen Wang, Lu Zhou, Tailing Wang, Xin Guo, Hao Yu, Jiaqi Wang
BACKGROUND: Rapid tissue expansion has been attempted, aiming at shortening the period of conventional expansion. However, it has scarcely been clinically applied because of its drawbacks such as low expansion efficiency and tissue destruction. Adipose-derived stem cell transplantation is a promising therapeutic method in regenerative medicine. However, its effects on rapid expansion remain poorly understood. METHODS: Twenty-four expanders were implanted in the dorsum of 12 pigs...
November 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/30511903/the-potential-chondrogenic-effect-of-e-autumnalis-aqueous-extracts-on-porcine-adipose-derived-mscs
#4
Makwese Johannes Maepa, Cornelius Canu Ssemakulu, Keolebogile Shirley Caroline M Motaung
Articular cartilage defects are common, causing significant pain and morbidities. To date, tissue engineering techniques using adipose-derived mesenchymal stem cells have offered a promising modality for cartilage repair. In this study, we investigated the effects of Eucomis autumnalis (E. autumnalis) aqueous crude extracts in porcine adipose-derived mesenchymal stem cells (pADMSCs) to determine whether they can differentiate pADMSCs into chondrocytes. Porcine ADMSCs were prepared and treated with different concentrations of E...
December 4, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/30511808/simulating-inflammation-in-a-wound-microenvironment-using-a-dermal-wound-on-a-chip-model
#5
Sahar Biglari, Thi Y L Le, Richard P Tan, Steven G Wise, Alessandro Zambon, Gaia Codolo, Marina De Bernard, Majid Warkiani, Aaron Schindeler, Sina Naficy, Peter Valtchev, Ali Khademhosseini, Fariba Dehghani
Considerable progress has been made in the field of microfluidics to develop complex systems for modeling human skin and dermal wound healing processes. While microfluidic models have attempted to integrate multiple cell types and/or 3D culture systems, to date they have lacked some elements needed to fully represent dermal wound healing. This paper describes a cost-effective, multicellular microfluidic system that mimics the paracrine component of early inflammation close to normal wound healing. Collagen and Matrigel are tested as materials for coating and adhesion of dermal fibroblasts and human umbilical vein endothelial cells (HUVECs)...
December 4, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/30510412/enhancement-of-wound-healing-by-single-wall-multi-wall-carbon-nanotubes-complexed-with-chitosan
#6
Naim Kittana, Mohyeddin Assali, Hanood Abu-Rass, Susanne Lutz, Rama Hindawi, Lina Ghannam, Marah Zakarneh, Ahmad Mousa
Background: Impaired wound healing is commonly associated with many health problems, including diabetes, bedsores and extensive burns. In such cases, healing often takes a long time, which subjects patients to various complications. This study aims to investigate whether single-wall or multi-wall carbon nanotubes complexed with chitosan hydrogel can improve wound healing. Materials and methods: Initially, the effects of the complexes on the viability and functionality of fibroblasts were investigated in engineered connective tissues...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/30509215/short-term-dexamethasone-treatment-transiently-but-not-permanently-attenuates-fibrosis-after-acute-to-chronic-kidney-injury
#7
Lies Moonen, Hilde Geryl, Patrick C D'Haese, Benjamin A Vervaet
BACKGROUND: Acute kidney injury (AKI) is an underestimated, yet important, risk factor for the development of chronic kidney disease (CKD). Persistence of inflammation after a renal ischemic injury has been observed, both in experimental models and patients, and is thought to be an important mechanisms underlying progression of acute-to-chronic renal injury. Temporary suppression of inflammation immediately after AKI might therefore be a good first-line therapeutic strategy towards a better long term outcome...
December 3, 2018: BMC Nephrology
https://www.readbyqxmd.com/read/30507548/titanium-dioxide-nanoparticle-gelatin-a-potential-burn-wound-healing-biomaterial
#8
Sara Javanmardi, Amaneh Ghojoghi, Baharak Divband, Javad Ashrafi
INTRODUCTION: Over the past few decades, the application of nanotechnology has gained progressive interest for the regeneration of injured and burned tissues. OBJECTIVE: This study evaluates the effects of titanium dioxide (TiO₂) nanoparticle (NP)/gelatin on burn wound healing in mice. MATERIALS AND METHODS: Sixty healthy male BALB/c mice with a full-thickness burn wound were randomized into 4 experimental groups of 15 animals each: (1) control group was treated with normal saline; (2) gelatin group was treated with gelatin-based ointment; (3) silver sulfadiazine group was treated with silver sulfadiazine 1% ointment; and (4) TiO₂ NP/gelatin group (TNG) received TiO₂ NP/gelatin...
December 2018: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/30505446/cell-secreted-extracellular-matrix-independent-of-cell-source-promotes-the-osteogenic-differentiation-of-human-stromal-vascular-fraction
#9
Jenna N Harvestine, Hakan Orbay, Jonathan Y Chen, David E Sahar, J Kent Leach
Lipoaspirates contain a readily accessible heterogeneous cell source for use in bone regeneration collectively referred to as the stromal vascular fraction (SVF). However, the osteogenic potential of SVF is inferior to other progenitor cell populations, thereby requiring alternative strategies to potentiate its effective use in cell-based therapies of bone repair. Cell-secreted extracellular matrix (ECM) is a promising substrate to guide cell phenotype or for use in biomaterial design, yet the instructional capacity of ECMs produced by various cell types is unknown...
June 28, 2018: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/30504760/hypoxia-inducible-factor-1-%C3%AE-hif-1%C3%AE-induces-apoptosis-of-human-uterosacral-ligament-fibroblasts-through-the-death-receptor-and-mitochondrial-pathways
#10
Xinrui Zhao, Lidong Liu, Rui Li, Xuan Wei, Wenqing Luan, Peishu Liu, Jing Zhao
BACKGROUND Hypoxia induces cell apoptosis in the uterosacral ligaments of patients with pelvic organ prolapse by upregulation of hypoxia-inducible factor-1α (HIF-1α). This study aimed to investigate the effects of HIF-1α on human uterosacral ligament fibroblasts (hUSLFs) following treatment with the chemical inducer of hypoxia, cobalt chloride (CoCl2), and to explore the underlying mechanisms. MATERIAL AND METHODS Ten women who underwent hysterectomy for benign disease provided uterosacral ligament tissue for cell extraction...
December 3, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/30501565/3d-co-culture-models-underline-metastatic-melanoma-cell-sensitivity-to-vemurafenib
#11
Delphine Morales, Florian Lombart, Agathe Truchot, Pauline Maire, Marwa Hussein, Warda Hamitou, Pascale Vigneron, Antoine Galmiche, Catherine Lok, Muriel Vayssade
Melanoma cell sensitivity to targeted therapy molecules is dependent on the tumor microenvironment (cell-cell and cell-extracellular matrix interactions). Three dimensional (3D) in vitro cell culture systems better reflect the native structural architecture of tissues and are attractive to investigate cellular interactions. We have developed and compared several metastatic melanoma models: melanoma cells (SK-MEL-28 and SK-MEL-3, BRAF V600E mutant and SK-MEL-2 BRAF wt) cultured as a monolayer (2D) and co-cultured on 3D dermal equivalents with fibroblasts to better unravel factors modulating cell sensitivity to a BRAF inhibitor (BRAFi, vemurafenib)...
December 1, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/30501564/primary-mucosal-epithelial-cell-cultivation-a-reliable-and-accelerated-isolation-routine
#12
Heide-Katharina Bauer, Daniela Flesch, Stefan Walenta, Ronald E Unger, Roxana Schwab, Sandra Nezi-Cahn, Annette Hasenburg, Martin Heller, Walburgis Brenner
BACKGROUND: Our aim was to establish a reliable protocol for the isolation of primary human oral mucosa epithelial cells that ensured a successful, efficient and reproducible cell culture for the utilization in subsequent experiments and for tissue engineering purposes. Therefore, we analyzed and evaluated various isolation routines and cultivation protocols. Furthermore, we investigated the functionality of isolated epithelial cells for tissue engineering approaches in an in vitro mucosa equivalent cell culture model system...
December 1, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/30500508/controlled-release-of-lawsone-from-polycaprolactone-gelatin-electrospun-nano-fibers-for-skin-tissue-regeneration
#13
Mahboobeh Adeli-Sardou, Mohammad Mehdi Yaghoobi, Masoud Torkzadeh-Mahani, Masoumeh Dodel
In this study, we aimed to evaluate the role of electrospun nanofibers containing lawsone (2-hydroxy-1,4-naphthoquinone) in wound healing. Different concentrations of lawsone (0.5%, 1%, 1.5%) were electrospun in polycaprolactone-gelatin (PCL/Gel) polymers in core-shell architecture. Nanofibers were characterized using scanning electron microscopy (SEM), Transmission electron microscopy (TEM). Coaxial electrospinning of PCL/Gel/lawsone (PCL/Gel/Law) scaffolds prolonged the release of lawsone over a period of 20 days...
November 27, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30500159/soft-hyaluronic-gels-promote-cell-spreading-stress-fibers-focal-adhesion-and-membrane-tension-by-phosphoinositide-signaling-not-traction-force
#14
Kalpana Mandal, Dikla Raz-Ben Aroush, Zachary Tobias Graber, Bin Wu, Chan Young Park, Jeffrey J Fredberg, Wei Guo, Tobias Baumgart, Paul A Janmey
Cells respond to both physical and chemical aspects of their substrate. Whether intracellular signals initiated by physical stimuli are fundamentally different from those elicited by chemical stimuli is an open question. Here we show that the requirement for a stiff substrate and therefore high cellular tension in order for cells to produce large focal adhesions and stress fibers is obviated when a soft substrate contains both hyaluronic acid (HA) and an integrin ligand (collagen I). HA is a major ECM component that is often upregulated during wound healing and tumor growth...
November 30, 2018: ACS Nano
https://www.readbyqxmd.com/read/30500138/a-double-blind-randomized-pilot-study-evaluating-the-safety-and-efficacy-of-topical-mep-in-the-facial-appearance-improvement-of-estrogen-deficient-females
#15
Zoe Diana Draelos
Facial skin aging is accelerated in postmenopausal females due to decreased collagen, reduced hydration, and loss of skin elasticity constituting the characteristics of estrogen deficient skin (EDS). The presence of estrogen receptors on dermal fibroblasts and epidermal keratinocytes confirms the role of estrogen in skin health. This research examined the efficacy and tolerability of topical methyl estradiolpropanoate (MEP) as an anti-aging cosmeceutical with estrogen like cutaneous effects in postmenopausal women who had never taken hormone replacement therapy (HRT)...
November 1, 2018: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/30486363/proteomics-of-bronchoalveolar-lavage-fluid-reveals-a-lung-oxidative-stress-response-in-murine-herpesvirus-68-infection
#16
Eric Bortz, Ting-Ting Wu, Parthive Patel, Julian P Whitelegge, Ren Sun
Murine herpesvirus-68 (MHV-68) productively infects mouse lungs, exhibiting a complex pathology characteristic of both acute viral infections and chronic respiratory diseases. We sought to discover proteins differentially expressed in bronchoalveolar lavage (BAL) from mice infected with MHV-68. Mice were infected intranasally with MHV-68. After nine days, as the lytic phase of infection resolved, differential BAL proteins were identified by two-dimensional (2D) electrophoresis and mass spectrometry. Of 23 unique proteins, acute phase proteins, vitamin A transport, and oxidative stress response factors Pdx6 and EC-SOD (Sod3) were enriched...
November 27, 2018: Viruses
https://www.readbyqxmd.com/read/30485610/pathogenesis-investigation-of-mir-199-5p-in-oral-submucous-fibrosis-based-on-bioinformatics-analysis
#17
Yongxiang Yuan, Ning Li, Lijuan Zeng, Zhengzhong Shen, Canhua Jiang
OBJECTIVES: Fibrosis diseases are one of the leading causes of suffering and death. However, no systematic investigation has been carried out on fibrosis-related genes. MATERIALS AND METHODS: By querying PubMed using keywords "fibrosis" and "gene" or "protein", we identified fibrosis-related genes in the last decade. Bioinformatics analysis was performed by MAS 3.0 software. Key miRNA was selected to assesse its relationship with oral submucous fibrosis (OSF) and fibroblast functions...
November 28, 2018: Oral Diseases
https://www.readbyqxmd.com/read/30485485/a-c-type-cpg-odn-accelerates-wound-healing-via-regulating-fibroblasts-and-immune-response
#18
Lina Li, Zhenyu Xu, Jian Zuo, Jin Ding
Abolished or delayed wound healing is a serious problem in clinical surgery, therefore, the new therapy for wound healing is needed. Synthetic oligodeoxynucleotides containing one or more CpG motifs (CpG ODN) has been reported to activate the immune system and improves skin wound healing. The aim of the present study was to evaluate the role of a new C-type CpG ODN in wound healing. We found that the CpG ODN promoted cell proliferation and collagen I production in human skin fibroblasts cells. Besides, we also investigated the effect of CpG ODN on the activation of immune cells...
November 28, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30485339/novel-high-throughput-myofibroblast-assays-identify-agonists-with-therapeutic-potential-in-pulmonary-fibrosis-that-act-via-ep2-and-ep4-receptors
#19
Patrick Sieber, Anny Schäfer, Raphael Lieberherr, François Le Goff, Manuel Stritt, Richard W D Welford, John Gatfield, Oliver Peter, Oliver Nayler, Urs Lüthi
Pathological features of pulmonary fibrosis include accumulation of myofibroblasts and increased extracellular matrix (ECM) deposition in lung tissue. Contractile α-smooth muscle actin (α-SMA)-expressing myofibroblasts that produce and secrete ECM are key effector cells of the disease and therefore represent a viable target for potential novel anti-fibrotic treatments. We used primary normal human lung fibroblasts (NHLF) in two novel high-throughput screening assays to discover molecules that inhibit or revert fibroblast-to-myofibroblast differentiation...
2018: PloS One
https://www.readbyqxmd.com/read/30484494/yap-activation-promotes-the-transdifferentiation-of-cardiac-fibroblasts-to-myofibroblasts-in-matrix-remodeling-of-dilated-cardiomyopathy
#20
Bo Jin, Jun Zhu, Hai-Ming Shi, Zhi-Chao Wen, Bang-Wei Wu
Yes-associated protein (YAP) is an important regulator of cellular proliferation and transdifferentiation. However, little is known about the mechanisms underlying myofibroblast transdifferentiation in dilated cardiomyopathy (DCM). We investigated the role of YAP in the pathological process of cardiac matrix remodeling. A classic model of DCM was established in BALB/c mice by immunization with porcine cardiac myosin. Cardiac fibroblasts were isolated from neonatal Sprague-Dawley rats by density gradient centrifugation...
November 23, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
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