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Keywords İnfertility ivf mitochondria ...

İnfertility ivf mitochondria sperm

https://read.qxmd.com/read/15256505/comparison-of-four-fluorochromes-for-the-detection-of-the-inner-mitochondrial-membrane-potential-in-human-spermatozoa-and-their-correlation-with-sperm-motility
#21
COMPARATIVE STUDY
Carole Marchetti, Nathalie Jouy, Brigitte Leroy-Martin, André Defossez, Pierre Formstecher, Philippe Marchetti
BACKGROUND: Sperm motility evaluation is associated with fertility in IVF programmes. The visual estimation of sperm motility is extremely subjective. Hence, alternative methods are required. Among them, determination of mitochondrial membrane potential (Deltapsi(m)) changes of spermatozoa using potentiometric dyes may be a reliable test to determine sperm quality. However, the use of the potentiometric dyes in sperm samples has not been compared. METHODS: We have studied sperm samples from 28 infertile patients enrolled in an IVF programme in flow cytometry after staining of spermatozoa with four commonly used potentiometric dyes...
October 2004: Human Reproduction
https://read.qxmd.com/read/13129747/-first-twins-born-in-mainland-china-by-autologous-granular-cell-mitochondria-transfer
#22
JOURNAL ARTICLE
Ling-hong Kong, Zhong Liu, Hong Li, Liang Zhu, Shi-ling Chen, Fu-qi Xing
A 37-year-old woman with the history of twice spontaneous abortion, who received unfruitful in vitro fertilization and embryo transfer (IVF-ET) for two cycles due to poor embryo quality, underwent the third cycle for autologous granular cell mitochondria transfer of 5 matured oocytes and intracytoplasmic sperm injection (ICSI) in another 4 oocytes. Better embryo quality was resulted by the former technique than by the latter, despite that fertilization was achieved in all the 4 oocytes with the latter operation...
September 2003: Di 1 Jun Yi da Xue Xue Bao, Academic Journal of the First Medical College of PLA
https://read.qxmd.com/read/12672125/ubiquitin-dependent-proteolysis-in-mammalian-spermatogenesis-fertilization-and-sperm-quality-control-killing-three-birds-with-one-stone
#23
REVIEW
Peter Sutovsky
Ubiquitin and ubiquitin-like proteins control the degradation of substrates as diverse as cyclins, viral envelope proteins, plasma membrane receptors, and mRNAs. The ubiquitinated substrates are targeted towards the lysosomal or proteasomal degradation sites. The number and position of ubiquitin molecules bound to substrates' lysine residues and the number and position of ubiquitin molecules in polyubiquitin chains determine the astonishing substrate specificity of ubiquitin-mediated proteolysis. Ubiquitin is likely to be expressed in mammalian gametes and embryos at any given developmental step, but the information on ubiquitin dependence of gametogenesis and fertilization is sketchy...
May 1, 2003: Microscopy Research and Technique
https://read.qxmd.com/read/12470525/successful-icsi-in-a-case-of-severe-asthenozoospermia-due-to-93-non-specific-axonemal-alterations-and-90-abnormal-or-absent-mitochondrial-sheaths
#24
JOURNAL ARTICLE
A Alosilla Fonttis, R Napolitano, M A Tomás
The use of IVF and intracytoplasmic sperm injection (ICSI) to treat male infertility due to severe asthenozoospermia in cases of electron microscopically confirmed axonemal abnormalities has been reported. However, even with these sophisticated technologies, very few pregnancies and births have been achieved with ejaculated spermatozoa in infertility due to this condition. The existence of sperm-derived defects preclude normal embryo development, and another reason for low success rates may be that the most commonly used method for selecting viable spermatozoa for ICSI is sperm motility, which is absent in affected men...
November 2002: Reproductive Biomedicine Online
https://read.qxmd.com/read/11980749/study-of-mitochondrial-membrane-potential-reactive-oxygen-species-dna-fragmentation-and-cell-viability-by-flow-cytometry-in-human-sperm
#25
JOURNAL ARTICLE
Carole Marchetti, Guillaume Obert, André Deffosez, Pierre Formstecher, Philippe Marchetti
BACKGROUND: Sperm cell death appears to be a cause of male infertility. The objective of this study was to determine the most reliable method for the evaluation of sperm quality in semen samples during sperm preparation for IVF. METHODS: Conventional analysis of semen samples was compared with several cytofluorometric methods detecting death-associated changes. Neat semen from infertile patients and sperm prepared by PureSperm gradient were studied by conventional microscopy and analysed for mitochondrial membrane potential (Delta Psi(m)), generation of reactive oxygen species, DNA fragmentation and cell viability...
May 2002: Human Reproduction
https://read.qxmd.com/read/11940662/asthenozoospermia-in-mice-with-targeted-deletion-of-the-sperm-mitochondrion-associated-cysteine-rich-protein-smcp-gene
#26
JOURNAL ARTICLE
Karim Nayernia, Ibrahim M Adham, Elke Burkhardt-Göttges, Jürgen Neesen, Mandy Rieche, Stephan Wolf, Ulrich Sancken, Kenneth Kleene, Wolfgang Engel
The sperm mitochondria-associated cysteine-rich protein (SMCP) is a cysteine- and proline-rich structural protein that is closely associated with the keratinous capsules of sperm mitochondria in the mitochondrial sheath surrounding the outer dense fibers and axoneme. To investigate the function of SMCP, we generated mice with a targeted disruption of the gene Smcp by homologous recombination. Homozygous mutant males on a mixed genetic background (C57BL/6J x 129/Sv) are fully fertile, while they are infertile on the 129/Sv background, although spermatogenesis and mating are normal...
May 2002: Molecular and Cellular Biology
https://read.qxmd.com/read/10407647/quantitative-ultramorphological-qum-analysis-of-human-sperm-diagnosis-and-management-of-male-infertility
#27
JOURNAL ARTICLE
B Bartoov, F Eltes, M Reichart, J Langzam, H Lederman, N Zabludovsky
The advantages of quantitative ultramorphological (QUM) sperm analysis in the diagnosis and treatment of male infertility are presented. QUM methodology is based on three elements: (1) complementary SEM and TEM observations of 7 sperm cell subcellular organelles: acrosome, postacrosomal lamina, nucleus, neck, axoneme, mitochondrial sheath, and outer dense fibers; (2) systematic classification of the specific ultramorphological malformations into 4 pathological and the normal categories, which indicate the morphological state of each subcellular organelle; and (3) comparison between well-defined reference groups with opposite fertility status or treatment conditions...
May 1999: Archives of Andrology
https://read.qxmd.com/read/9266524/the-effect-of-coenzyme-q10-on-sperm-motility-and-function
#28
JOURNAL ARTICLE
A Lewin, H Lavon
In sperm cells, the majority of coenzyme Q10 (CoQ10) an energy promoting agent and antioxidant, is concentrated in the mitochondria of the midpiece, so that the energy for movement and all other energy-dependent processes in the sperm cell also depend on the availability of CoQ10. The reduced form of CoQ10-ubiquinol also acts as an antioxidant, preventing lipid peroxidation in sperm membranes. The objective of the study was to evaluate the effect of CoQ10 on sperm motility in vitro, after incubation with 38 samples of asthenospermic and normal motility sperm, and to evaluate the effect of CoQ10 administration in vivo in 17 patients with low fertilization rates after in vitro fertilization with intracytoplasmic sperm injection (ICSI) for male factor infertility...
1997: Molecular Aspects of Medicine
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