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https://www.readbyqxmd.com/read/30097608/a-recombinant-newcastle-disease-virus-ndv-expressing-s-protein-of-infectious-bronchitis-virus-ibv-protects-chickens-against-ibv-and-ndv
#1
Edris Shirvani, Anandan Paldurai, Vinoth K Manoharan, Berin P Varghese, Siba K Samal
Infectious bronchitis virus (IBV) causes a highly contagious respiratory, reproductive and urogenital tract disease in chickens worldwide, resulting in substantial economic losses for the poultry industry. Currently, live-attenuated IBV vaccines are used to control the disease. However, safety, attenuation and immunization outcomes of current vaccines are not guaranteed. Several studies indicate that attenuated IBV vaccine strains contribute to the emergence of variant viruses in the field due to mutations and recombination...
August 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30085298/safety-and-efficacy-of-attenuated-classic-and-variant-2-infectious-bronchitis-virus-candidate-vaccines
#2
Ahmed Ali, Walid H Kilany, Mohamed A Zain El-Abideen, Magdy El Sayed, Magdy Elkady
Vaccination programs against infectious bronchitis virus (IBV) in Egypt depend on both classical and/or imported variant IBV strain vaccines. However, many IBV outbreaks associated with respiratory distress, nephropathy, and high mortalities were attributed to the circulation of both classical and new nephropathogenic IBV variant 2 strains. In the present study, we report the development of attenuated IBV candidate vaccines using the classic IBV strains (IBM41 and IB2) and a nephropathogenic strain (IBvar2)...
August 6, 2018: Poultry Science
https://www.readbyqxmd.com/read/30067172/deletion-of-accessory-genes-3a-3b-5a-or-5b-from-avian-coronavirus-infectious-bronchitis-virus-induces-an-attenuated-phenotype-both-in-vitro-and-in-vivo
#3
Andrea Laconi, Steven J van Beurden, Alinda J Berends, Annika Krämer-Kühl, Christine A Jansen, Dieuwertje Spekreijse, Gilles Chénard, Hans-Christian Philipp, Egbert Mundt, Peter J M Rottier, M Hélène Verheije
Avian coronavirus infectious bronchitis virus (IBV) infects domestic fowl, resulting in respiratory disease and causing serious losses in unprotected birds. Its control is mainly achieved by using live attenuated vaccines. Here we explored the possibilities for rationally attenuating IBV to improve our knowledge regarding the function of IBV accessory proteins and for the development of next-generation vaccines with the recently established reverse genetic system for IBV H52 based on targeted RNA recombination and selection of recombinant viruses in embryonated eggs...
August 1, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/30052648/surveillance-for-respiratory-and-diarrheal-pathogens-at-the-human-pig-interface-in-sarawak-malaysia
#4
Laura K Borkenhagen, Kerry A Mallinson, Rick W Tsao, Siaw-Jing Ha, Wei-Honn Lim, Teck-Hock Toh, Benjamin D Anderson, Jane K Fieldhouse, Sarah E Philo, Kuek-Sen Chong, William G Lindsley, Alejandro Ramirez, James F Lowe, Kristen K Coleman, Gregory C Gray
BACKGROUND: The large livestock operations and dense human population of Southeast Asia are considered a hot-spot for emerging viruses. OBJECTIVES: To determine if the pathogens adenovirus (ADV), coronavirus (CoV), encephalomyocarditis virus (EMCV), enterovirus (EV), influenza A-D (IAV, IBV, ICV, and IDV), porcine circovirus 2 (PCV2), and porcine rotaviruses A and C (RVA and RVC), are aerosolized at the animal-interface, and if humans working in these environments are carrying these viruses in their nasal airways...
2018: PloS One
https://www.readbyqxmd.com/read/30044776/susceptibility-of-influenza-viruses-to-hypothiocyanite-and-hypoiodite-produced-by-lactoperoxidase-in-a-cell-free-system
#5
Urmi Patel, Aaron Gingerich, Lauren Widman, Demba Sarr, Ralph A Tripp, Balázs Rada
Lactoperoxidase (LPO) is an enzyme found in several exocrine secretions including the airway surface liquid producing antimicrobial substances from mainly halide and pseudohalide substrates. Although the innate immune function of LPO has been documented against several microbes, a detailed characterization of its mechanism of action against influenza viruses is still missing. Our aim was to study the antiviral effect and substrate specificity of LPO to inactivate influenza viruses using a cell-free experimental system...
2018: PloS One
https://www.readbyqxmd.com/read/30041895/effects-of-an-ergonomic-program-on-the-quality-of-life-and-work-performance-of-university-staff-with-physical-disabilities-a-clinical-trial-with-three-month-follow-up
#6
Maria Ángela Ramalho-Pires de Almeida, Gracia Maria Ábalos-Medina, Carmen Villaverde-Gutiérrez, Neide Maria Gomes-de Lucena, Alecsandra Ferreira-Tomaz, Jose Manuel Perez-Marmol
BACKGROUND: Problems related to physical disability may have an extremely negative impact in the work environment, reducing productivity and contributing to health problems and a worsening quality of life. OBJECTIVE: To assess the effects of an ergonomic intervention program on the quality of life and the work performance of people with physical disabilities working in a university environment. METHODS: A pilot clinical trial with three-month follow-up was conducted at the Physiotherapy Clinic of the Federal University of Paraíba (Brazil)...
July 18, 2018: Disability and Health Journal
https://www.readbyqxmd.com/read/30022779/immune-protection-conferred-by-three-commonly-used-commercial-live-attenuated-vaccines-against-the-prevalent-local-strains-of-avian-infectious-bronchitis-virus-in-southern-china
#7
Wen-Sheng Fan, He-Ming Li, Yi-Ning He, Ning Tang, Li-Hua Zhang, Hai-Yong Wang, Lian Zhong, Jian-Cai Chen, Tian-Chao Wei, Teng Huang, Mei-Lan Mo, Ping Wei
Live attenuated vaccines are critical in the control of avian infectious bronchitis. It is necessary to know the protection conferred by commonly used commercial live vaccines. In this study, specific pathogen-free chicks were vaccinated with the commercial live vaccines H120, 4/91 and LDT3-A. Blood samples were collected at weekly intervals for the detection of IBV-specific antibodies and quantification of CD4+ and CD8+ T lymphocytes. At 21 days post-inoculation the vaccinated birds were challenged with the IBV prevalent local strains GX-YL5, GX-GL11079 and GX-NN09032, respectively...
July 18, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30021894/the-s2-subunit-of-infectious-bronchitis-virus-beaudette-is-a-determinant-of-cellular-tropism
#8
Erica Bickerton, Helena J Maier, Phoebe Stevenson-Leggett, Maria Armesto, Paul Britton
The spike glycoprotein (S) of the avian gammacoronavirus, infectious bronchitis virus (IBV), is comprised of two subunits, S1 and S2, and has a role in virulence in vivo as well as being responsible for cellular tropism in vitro We have previously demonstrated that replacement of the S glycoprotein ectodomain from the avirulent Beaudette strain of IBV with the corresponding region from the virulent M41-CK strain resulted in a recombinant virus, BeauR-M41(S), with the in vitro cell tropism of M41-CK. The IBV Beaudette strain is able to replicate in both primary chick kidney cells and Vero cells, whereas the IBV M41-CK strain replicates in primary cells only...
July 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29996007/influenza-b-viruses-in-pigs-taiwan
#9
Ching-Ping Tsai, Hsiang-Jung Tsai
BACKGROUND: Influenza B viruses (IBVs) have never been isolated from natural-infected pigs in clinical cases, although the susceptibility of domestic pigs to experimental IBV infections had been confirmed as well as IBV-specific antibodies were detected from pigs under natural and experimental conditions. OBJECTIVES: We aimed to assess and investigate the activities for infection and circulation of IBVs in pigs. METHODS: Annual active surveys for influenza have been implemented on swine populations in Taiwan since July 1998...
July 11, 2018: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/29987628/production-of-recombinant-n-protein-of-infectious-bronchitis-virus-using-the-baculovirus-expression-system-and-its-assessment-as-a-diagnostic-antigen
#10
Huseyin Yilmaz, Bonto Faburay, Nuri Turan, Maira Cotton-Caballero, Burhan Cetinkaya, Aydin Gurel, Aysun Yilmaz, Utku Y Cizmecigil, Ozge Aydin, Eda Altan Tarakci, Erhan Bayraktar, Juergen A Richt
The avian coronavirus-infectious bronchitis virus (AvCoV-IBV) is recognized as an important avian pathogen, and new viral variants are a continuous threat to the poultry industry worldwide. Sensitive diagnostics and efficacious vaccines are necessary to combat IBV infections in chickens. The aim of this study was to produce recombinant N protein of IBV in the baculovirus system to use in ELISA diagnostic tests in order to enable the assessment of the sero-prevalence and risk of IBV infections in chickens in Turkey...
July 9, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29954092/protection-against-virulent-infectious-bronchitis-virus-challenge-conferred-by-a-recombinant-baculovirus-co-expressing-s1-and-n-proteins
#11
Yuan Yuan, Zhi-Peng Zhang, Yi-Ning He, Wen-Sheng Fan, Zhi-Hua Dong, Li-Hua Zhang, Xin-Kuan Sun, Li-Li Song, Tian-Chao Wei, Mei-Lan Mo, Ping Wei
Avian infectious bronchitis virus (IBV) is the causative agent of infectious bronchitis, which results in considerable economic losses. It is imperative to develop safe and efficient candidate vaccines to control IBV infection. In the current study, recombinant baculoviruses co-expressing the S1 and N proteins and mono-expressing S1 or N proteins of the GX-YL5 strain of IBV were constructed and prepared into subunit vaccines rHBM-S1-N, rHBM-S1 and rHBM-N. The levels of immune protection of these subunit vaccines were evaluated by inoculating specific pathogen-free (SPF) chickens at 14 days of age, giving them a booster with the same dose 14 days later and challenging them with a virulent GX-YL5 strain of IBV 14 days post-booster (dpb)...
June 27, 2018: Viruses
https://www.readbyqxmd.com/read/29951960/non-invasive-cerebrovascular-autoregulation-assessment-using-the-volumetric-reactivity-index-prospective-study
#12
Vytautas Petkus, Aidanas Preiksaitis, Solventa Krakauskaite, Laimonas Bartusis, Romanas Chomskis, Yasin Hamarat, Erika Zubaviciute, Saulius Vosylius, Saulius Rocka, Arminas Ragauskas
BACKGROUND: This prospective study of an innovative non-invasive ultrasonic cerebrovascular autoregulation (CA) monitoring method is based on real-time measurements of intracranial blood volume (IBV) reactions following changes in arterial blood pressure. In this study, we aimed to determine the clinical applicability of a non-invasive CA monitoring method by performing a prospective comparative clinical study of simultaneous invasive and non-invasive CA monitoring on intensive care patients...
June 27, 2018: Neurocritical Care
https://www.readbyqxmd.com/read/29945221/effects-of-dietary-yeast-nucleotides-supplementation-on-intestinal-barrier-function-intestinal-microbiota-and-humoral-immunity-in-specific-pathogen-free-chickens
#13
Che Wu, Zhenwei Yang, Cailiang Song, Chao Liang, Hongxin Li, Weiguo Chen, Wencheng Lin, Qingmei Xie
Yeast nucleotides are a fine functional additive in human and animals. The effects of dietary yeast nucleotides supplementation on intestinal development, expression of intestinal barrier-related genes, intestinal microbiota, and infectious bronchitis virus (IBV) antibody titer of specific pathogen-free (SPF) chickens were investigated. A total of 60 1-d-old chickens were divided into 4 groups, each of which included 3 replicates of 5 chickens. Group 1 served as a control that was fed a basal diet. Groups 2 to 4 were fed the basal diet supplemented with 0...
June 26, 2018: Poultry Science
https://www.readbyqxmd.com/read/29944406/identification-of-novel-linear-epitopes-located-in-the-infectious-bronchitis-virus-spike-s2-region
#14
Kiyohiko Andoh, Kanako Ashikaga, Kiyotaka Suenaga, Shun Endo, Kenichi Yamazaki
We identified novel linear epitopes on the infectious bronchitis virus (IBV) spike S2 region. The conformational structure of the IBV spike protein was predicted from a homologous protein, human coronavirus NL63 spike. Although the obtained structure was incomplete, most of the IBV spike protein structure was predicted; the N-terminus of the S1 region could not be predicted due to its variability. In the model, the region located in the proximity of the fusion peptide appeared to be well conserved, and we evaluated the antigenicity of these domains, which are involved in the membrane fusion machinery...
June 2018: Avian Diseases
https://www.readbyqxmd.com/read/29944405/using-multinomial-and-space-time-permutation-models-to-understand-the-epidemiology-of-infectious-bronchitis-in-california-between-2008-and-2012
#15
O Alejandro Aleuy, Maurice Pitesky, Rodrigo Gallardo
Although infectious bronchitis virus (IBV) has been described as one of the most economically important viral respiratory diseases in poultry, there are few analyses of outbreaks that use spatial statistics. In order to better understand how the different genotypes of IBV behave spatially and temporally, we used geographic information system-based mapping coupled with spatial and spatial-temporal statistics to identify statistically significant clustering of multiple strains of infectious bronchitis (IB) between 2008 and 2012 in California...
June 2018: Avian Diseases
https://www.readbyqxmd.com/read/29943528/-research-advance-of-influenza-b-viruses
#16
Pengtao Jiao, Wenhui Fan, Yeping Sun, Wenjun Liu, Jing Li
Influenza B virus (IBV) is a segmented negative-strand RNA virus, which often causes local outbreak or seasonal epidemic along with influenza A virus (IAV) in the world. It is pathogenic to children, teenagers and elderly people and has a higher mortality rate in children and adolescents, so it poses a serious threat to public health and health. IBV is more likely to cause complications than IAV and the disease burden of IBV even exceeds IAV in the epidemic season. Recently, especially after winter of 2017, IBV has become the dominant strain in many areas of our country and seriously affects people's health...
June 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29940196/viral-interference-between-low-pathogenic-avian-influenza-h9n2-and-avian-infectious-bronchitis-viruses-in-vitro-and-in-ovo
#17
Rim Aouini, Nacira Laamiri, Abdeljelil Ghram
BACKGROUND: Low pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated chicken eggs (ECE). Chicken embryo lung (CEL) and ECE were simultaneously or sequentially infected with IBV vaccine strain (H120) and LPAIV-H9N2 (A/Ck/TUN/145/2012) to evaluate viral interactionsin vitro and in ovo, respectively...
September 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29922421/seroprevalence-and-genotyping-of-avian-infectious-bronchitis-virus-detected-from-iranian-unvaccinated-backyard-chickens
#18
Shima Shokri, Vahid Karimi, Arash Ghalyanchi Langeroudi, Mehdi Vasfi Marandi, Masoud Hashamzadeh, Taha Zabihipetroudi, Hamideh Najafi, Farshad Tehrani
Background and Objectives: Different epidemiological studies have found that backyard chickens are a reservoir for poultry diseases. Most backyard chicken flocks have a poor level of biosecurity, which increases the risk of spread of diseases. In recent years, the number of backyard chickens has been on the rise in Iran. However, the health status of backyard flocks is still poorly documented. Thus, this study aimed at examining the seroprevalence of antibodies against infectious bronchitis virus (IBV) and molecular surveillance and genotyping of IBV among backyard chickens (without vaccination history) in Mazandaran province, North of Iran, 2014...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29920114/in-vitro-phagocytosis-of-opsonized-latex-beads-by-hd11-cells-as-a-method-to-assess-the-general-opsonization-potential-of-chicken-serum
#19
Mohammed Naghizadeh, Eva Wattrang, Rikke B Kjærup, Maja Bakke, Sandra Shih, Tina S Dalgaard
Opsonins, an important arm of the innate immune system, are various soluble proteins, which play a critical role in destruction of invading pathogens directly or via engulfment of pathogens through the intermediate of phagocytosis. The diversity of opsonin profiles is under genetic influence and may be associated with variation in disease resistance. The aim of this study was to set up an assay to determine serum opsonophagocytic potential (OPp) for chicken sera by flow cytometry and to evaluate the assay using samples from different chicken lines...
July 9, 2018: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/29917155/molecular-characteristic-and-pathogenicity-analysis-of-a-virulent-recombinant-avain-infectious-bronchitis-virus-isolated-in-china
#20
K Y Feng, T Chen, X Zhang, G M Shao, Y Cao, D K Chen, W C Lin, F Chen, Q M Xie
A virulent infectious bronchitis virus (IBV), designated as CK/CH/GD/QY16 (referred as QY16), was isolated from a diseased chicken farm in Guangdong province, China, in 2016. The complete genome of the strain was sequenced and analyzed. The results show that the genome of QY16 consists of 27,670 nucleotides, excluding poly (A) tail, and that its genome organization is 5' UTR-1a-1b-S-3a-3b-E-M-4b-4c-5a-5b-N-6b-3' UTR-poly (A) tail. Sequence comparison among QY16 and other IBV strains was conducted and its results demonstrate that the S1 gene of QY16 has the highest nucleotide sequence identity with that of 4/91, and the other part of its genome is highly similar to that of YX10...
June 15, 2018: Poultry Science
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