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Rinkesh Kumar Gupta, Akanksha Sharma, Ajay Verma, Irfan Ahmad Ansari, Premendra D Dwivedi
In the course of analyzing amino acid sequence of an allergen (≈20 kDa), we found this protein has a homology with the amino acid sequence of putative α-Dioxygenase fragment (ADF). Allergy caused by many allergens having an enzymatic activity have been reported previously, but allergenicity to neither α-Dioxygenase enzyme nor to it's any constituents has been reported. We sought to purify an ADF (≈19.5 kDa) from chickpea to investigate it's inherent allergic potential in BALB/c mice. The ADF showed IgE-affinity in sera of sensitized BALB/c mice and allergic patients...
November 15, 2018: Immunobiology
Edmond S Chan, Matthew J Greenhawt, David M Fleischer, Jean-Christoph Caubet
Peanut is an allergenic legume that can cross-react with other plant-based foods, notably other legumes and tree nuts. Peanut allergic individuals can be both co-sensitized as well as co-allergic to such items, requiring foresight when eliciting a clinical history of reaction, in the diagnostic evaluation of such allergies, and in the counseling of patients as to food avoidances after a diagnosis is made. Legume allergens belong to the Fabaceae family and encompass the cupin, prolamin, PR-10, and LTP families, which mediate cross-sensitization including that between peanut and tree nut...
November 22, 2018: Journal of Allergy and Clinical Immunology in Practice
Zhankui Wang, Ming Chen, Yaqin Zhang, Liyan Huang, Shuang Wang, Yuan Tao, Peipei Qian, Abudoukeyumu Mijiti, Aixing Gu, Hua Zhang, Shubing Shi, Hui Cheng, Yun Wu, Langtao Xiao, Hao Ma
Proteinaceous α-amylase inhibitors have specialized activities that make some strong inhibition of α-amylases. New α-amylase inhibitors continue to be discovered so far. A proteinaceous α-amylase inhibitor CL-AI was isolated and identified from chickpea seeds. CL-AI, encoded by Q9SMJ4, was a storage legumin precursor containing one α-chain and one β-chain, and each chain possessed a same conserved cupin domain. Amino acid mutation and deficiency of cupin domain would lead to loss of α-amylase inhibitory activity, indicating that it was essential for inhibitory activity...
December 2018: Plant Science: An International Journal of Experimental Plant Biology
Gulshan Mahajan, Navneet Kaur Mutti, Prashant Jha, Michael Walsh, Bhagirath Singh Chauhan
Brassica tournefortii is an important broadleaf weed of the winter season in the northern grain region of Australia. Knowledge of germination ecology of this weed would help in implementing effective weed control programs. A series of experiments were conducted to study the germination and dormancy behavior of four biotypes of B. tournefortii seeds, biotypes A (collected from barley crop), B (barley fence lines), C (chickpea crop), and D (chickpea fence lines), collected from the St George region of Queensland...
November 20, 2018: Scientific Reports
Sreya Ghosh, Amy Watson, Oscar E Gonzalez-Navarro, Ricardo H Ramirez-Gonzalez, Luis Yanes, Marcela Mendoza-Suárez, James Simmonds, Rachel Wells, Tracey Rayner, Phon Green, Amber Hafeez, Sadiye Hayta, Rachel E Melton, Andrew Steed, Abhimanyu Sarkar, Jeremy Carter, Lionel Perkins, John Lord, Mark Tester, Anne Osbourn, Matthew J Moscou, Paul Nicholson, Wendy Harwood, Cathie Martin, Claire Domoney, Cristobal Uauy, Brittany Hazard, Brande B H Wulff, Lee T Hickey
'Speed breeding' (SB) shortens the breeding cycle and accelerates crop research through rapid generation advancement. SB can be carried out in numerous ways, one of which involves extending the duration of plants' daily exposure to light, combined with early seed harvest, to cycle quickly from seed to seed, thereby reducing the generation times for some long-day (LD) or day-neutral crops. In this protocol, we present glasshouse and growth chamber-based SB approaches with supporting data from experimentation with several crops...
December 2018: Nature Protocols
Antonio Carapelli, Abir Soltani, Chiara Leo, Matteo Vitale, Moez Amri, Jouda Mediouni-Ben Jemâa
Leafminer insects of the genus Liriomyza are small flies whose larvae feed on the internal tissue of some of the most important crop plants for the human diet. Several of these pest species are highly uniform from the morphological point of view, meaning molecular data represents the only reliable taxonomic tool useful to define cryptic boundaries. In this study, both mitochondrial and nuclear molecular markers have been applied to investigate the population genetics of some Tunisian populations of the polyphagous species Liriomyza cicerina , one of the most important pest of chickpea cultivars in the whole Mediterranean region...
November 15, 2018: Genes
Wahiba Amri-Tiliouine, Meriem Laouar, Aissa Abdelguerfi, Joanna Jankowicz-Cieslak, Ljupcho Jankuloski, Bradley J Till
In order to increase genetic variability for chickpea improvement, the Kabuli genotype, variety Ghab4, was treated with 280 Grays of gamma rays (Cobalt 60). Field characterization began with the M2 generation. A total of 135 M2 families were sown in the field resulting in approximately 4,000 plants. Traits related to phenology (days to flowering, days to maturity), plant morphology of vegetative parts (plant height, height of first pod, number of primary branches per plant) and yield (number of seeds per pod, total number of pods per plant, total number of seeds per plant, seed yield and hundred seed weight) were recorded and analyzed to evaluate genetic variability...
2018: Frontiers in Plant Science
Mohan Singh Rajkumar, Rohini Garg, Mukesh Jain
Seed size/weight is a major agronomic trait which determine crop productivity in legumes. To understand the genetic basis of seed size determination, we sought to identify DNA polymorphisms between two small (Himchana 1 and Pusa 362) and two large-seeded (JGK 3 and PG 0515) chickpea cultivars via whole genome resequencing. We identified a total of 75535 single nucleotide polymorphisms (SNPs), 6486 insertions and deletions (InDels), 1938 multi-nucleotide polymorphisms (MNPs) and 5025 complex variants between the two small and two large-seeded chickpea cultivars...
November 14, 2018: Scientific Reports
József Vuts, Christine M Woodcock, Lisa König, Stephen J Powers, John A Pickett, Árpád Szentesi, Michael A Birkett
The mechanisms of host shift in phytophagous insects are poorly understood. Among the many proposed processes involved, sexual selection via semiochemicals has recently been suggested. This hypothesizes that sexual communication using pheromones is modified as a result of development on a new host, and such plant-induced phenotypic divergence in mate recognition cues can lead to reproductive isolation between host lines. We tested this hypothesis on Acanthoscelides obtectus, an oligophagous bruchid of Phaseolus vulgaris beans worldwide, which also develops in acceptable non-hosts, such as chickpea (Cicer arietinum L...
2018: PloS One
Sameer Suresh Bhagyawant, Ajay Kumar Gautam, Dakshita Tanaji Narvekar, Neha Gupta, Amita Bhadkaria, Nidhi Srivastava, Hari D Upadhyaya
The seeds of chickpea provide an exceptional source of dietary proteins and is one of the important legumes in both developed and developing countries over the world. The available germplasm of cultivated chickpea is deficient in desired biochemical signatures. To identify new sources of variations for breeding, reduced subsets of germplasm such as mini-core collection can be explored as an effective resource. In the present investigation, mini-core collections consisting of 215 accessions of chickpea were extensively evaluated for tapping biochemical diversity...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Ahmed M S Hussein, Mona M Hussein, Manal F Salama, Ibrahim M Hamed, Karem Aly Fouda, Rasha S Mohamed
BACKGROUND AND OBJECTIVE: School children especially in Egypt need a safe meal which is able to meet their daily nutrient needs and ameliorate cognition. So, the current study aimed to evaluate formula prepared as cookies to be served as a meal for primary school children. MATERIALS AND METHODS: Wheat, roasted chickpea, milk protein concentrate, cinnamon and brewer's yeast were used to prepare cookies which have been exposed to sensory, chemical and biological evaluation...
January 2018: Pakistan Journal of Biological Sciences: PJBS
Elif Yaver, Nermin Bilgiçli
The influence of different dephytinisation methods such as malt flour addition, phytase enzyme application and citric acid addition on some properties of commercial bread and traditional flat bread containing 25% of cereal-legume (rye, barley, oat, chickpea, soy and lupin) flours was investigated. Ash, protein, crude fat, antioxidant activity, total phenolic content and mineral matters of both breads increased with the usage of cereal-legume flour blend. Cereal-legume flour blend also resulted in increments on phytic acid content of commercial bread (330 mg/100 g) and traditional flat bread (422 mg/100 g) compared to its controls (98 and 152 mg/100 g, respectively)...
March 15, 2019: Food Chemistry
Marielle Margier, Stéphane Georgé, Noureddine Hafnaoui, Didier Remond, Marion Nowicki, Laure Du Chaffaut, Marie-Josèphe Amiot, Emmanuelle Reboul
Pulses display nutritional benefits and are recommended in sustainable diets. Indeed, they are rich in proteins and fibers, and can contain variable amounts of micronutrients. However, pulses also contain bioactive compounds such as phytates, saponins, or polyphenols/tannins that can exhibit ambivalent nutritional properties depending on their amount in the diet. We characterized the nutritional composition and bioactive compound content of five types of prepared pulses frequently consumed in France (kidney beans, white beans, chickpeas, brown and green lentils, flageolets), and specifically compared the effects of household cooking vs...
November 4, 2018: Nutrients
Drishti Mandal, Senjuti Sinharoy
Mesorhizobium sp. produces root nodules in chickpea. Chickpea and model legume Medicago truncatula are members of inverted repeat lacking clade (IRLC). The rhizobia after internalization into the plant cell are called 'bacteroid'. Nodule Specific Cysteine-rich (NCR) peptides in IRLC legumes guide bacteroids to a 'terminally differentiated swollen (TDS)' form. Bacteroids in chickpea are less TDS than those in Medicago. Nodule development in chickpea indicates recent evolutionary diversification and merits further study...
November 6, 2018: Molecular Plant-microbe Interactions: MPMI
Ha Duc Chu, Kien Huu Nguyen, Yasuko Watanabe, Dung Tien Le, Thu Ly Thi Pham, Keiichi Mochida, Lam-Son Phan Tran
In plants, the Nuclear Factor-Y (NF-Y) transcription factors (TFs), which include three distinct types of NF-YA, NF-YB, and NF-YC TFs, have been identified to play key roles in the regulation of various plant growth and developmental processes under both normal and environmental stress conditions. In this work, a total of 40 CaNF-Y-encoding genes, including eight CaNF-YA s, 21 CaNF-YB s, and 11 CaNF-YC s, were identified in chickpea, and their major gene and protein characteristics were subsequently obtained using various web-based tools...
October 23, 2018: International Journal of Molecular Sciences
Joydeep Chakraborty, Prithwi Ghosh, Senjuti Sen, Sampa Das
Promoters of many defense related genes are enriched with W-box elements serving as binding sites for plant specific WRKY transcription factors. In this study, expression of WRKY40 transcription factor was analyzed in two contrasting susceptible (JG62) and resistant (WR315) genotypes of chickpea infected with Foc1. The resistant plants showed up-regulation of WRKY40 under Fusarium stress, whereas in susceptible plants WRKY40 expression was absent. Additionally, global changes in the histone modification patterns were studied in above two chickpea genotypes by immunoblotting and real-time PCR analyses under control and Fusarium infected conditions...
November 2018: Plant Science: An International Journal of Experimental Plant Biology
Joydeep Chakraborty, Prerna Priya, Shubhra Ghosh Dastidar, Sampa Das
Fusarium wilt is one of the most serious diseases affecting chickpea (Cicer arietinum L.). Here, we identified a putative Resistance Gene Analog (CaRGA) from chickpea, encoding a coiled-coil (CC) nucleotide-binding oligomerization domain (NB-ARC) containing leucine-rich repeat (LRR) protein (CC-NLR protein) that confers resistance against Fusarium oxysporum f. sp. ciceri race1 (Foc1). Over-expression and silencing of CaRGA in chickpea resulted in enhanced resistance and hyper-susceptibility, respectively against Foc1...
November 2018: Plant Science: An International Journal of Experimental Plant Biology
Sahar Dandachy, Hiba Mawlawi, Marwan Chedid, Carla El-Mallah, Omar Obeid
Recently, there has been an increasing interest in integrating pulse flours into pastries and baked products to improve their nutritional and health benefits. " Mankoushe ," a popular Lebanese pastry made up of refined wheat flour was enriched with chickpea flour that is of better nutritional value, and its postprandial glycemia, insulinemia, lipidemia and appetite measures were monitored. A randomized cross-over study was performed on sixteen healthy Lebanese females, age (years): 22.90 ± 3.00, and BMI (kg/m²): 22...
October 19, 2018: Foods (Basel, Switzerland)
Kevin Walsh, Nuala Calder, Peter Olupot-Olupot, Tonny Ssenyondo, William Okiror, Charles Bernard Okalebo, Rita Muhindo, Ayub Mpoya, Elaine Holmes, Julian Marchesi, Gael Delamare de la Villenaise de Chenevarin, Gary Frost, Kathryn Maitland
Background: Changes in intestinal mucosal integrity and gut microbial balance occur in severe acute malnutrition (SAM), resulting in treatment failure and adverse clinical outcomes (gram-negative sepsis, diarrhoea and high case-fatality). Transient lactose intolerance, due to loss of intestinal brush border lactase, also complicates SAM, thus milk based feeds may not be optimal for nutritional rehabilitation. Since the gut epithelial barrier can be supported by short chain fatty acids, derived from microbiota fermentation by particular fermentable carbohydrates, we postulated that an energy-dense nutritional feed comprising of legume-based fermentable carbohydrates, incorporated with lactose-free versions of standard World Health Organization (WHO) F75/F100 nutritional feeds will enhance epithelial barrier function in malnourished children, reduce and promote resolution of diarrhoea and improve overall outcome...
2018: Wellcome Open Research
Vanika Garg, Aamir W Khan, Himabindu Kudapa, Sandip M Kale, Annapurna Chitikineni, Sun Qiwei, Mamta Sharma, Chuanying Li, Baohong Zhang, Xin Liu, P B Kavi Kishor, Rajeev K Varshney
Ascochyta blight (AB) is one of the major biotic stresses known to limit the chickpea production worldwide. To dissect the complex mechanisms of AB resistance in chickpea, three approaches, namely, transcriptome, small RNA and degradome sequencing were used. The transcriptome sequencing of 20 samples including two resistant genotypes, two susceptible genotypes and one introgression line under control and stress conditions at two time points (3rd and 7th day post inoculation) identified a total of 6767 differentially expressed genes (DEGs)...
October 16, 2018: Plant Biotechnology Journal
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