Ze-Hua Wu, Xingfu Zhu, Qiqi Yang, Yulian Zagranyarski, Krishna Mishra, Hilmar Strickfaden, Ronald P Wong, Thomas Basché, Kaloian Koynov, Mischa Bonn, Chen Li, Xiaomin Liu, Klaus Müllen
Organic near-infrared (NIR) photoblinking fluorophores are highly desirable for live-cell super-resolution imaging based on single-molecule localization microscopy (SMLM). Herein we introduce a novel small chromophore, PMIP , through the fusion of perylenecarboximide with 2,2-dimetheylpyrimidine. PMIP exhibits an emission maximum at 732 nm with a high fluorescence quantum yield of 60% in the wavelength range of 700-1000 nm and excellent photoblinking without any additives. With resorcinol-functionalized PMIP ( PMIP-OH ), NIR SMLM imaging of lysosomes is demonstrated for the first time in living mammalian cells under physiological conditions...
March 5, 2024: Journal of the American Chemical Society