Teressa M Shaw, Samuel T Dettle, Andres Mejia, Jennifer M Hayes, Heather A Simmons, Puja Basu, Jens H Kuhn, Mitchell D Ramuta, Cody J Warren, Peter B Jahrling, David H O'Connor, Liupei Huang, Misbah Zaeem, Jiwon Seo, Igor I Slukvin, Matthew E Brown, Adam L Bailey
Genetically diverse simian arteriviruses (simarteriviruses) naturally infect geographically and phylogenetically diverse monkeys, and cross-species transmission and emergence are of considerable concern. Characterization of most simarteriviruses beyond sequence analysis has not been possible because the viruses fail to propagate in the laboratory. We attempted to isolate 4 simarteriviruses, Kibale red colobus virus 1, Pebjah virus, simian hemorrhagic fever virus, and Southwest baboon virus 1, by inoculating an immortalized grivet cell line (known to replicate simian hemorrhagic fever virus), primary macaque cells, macrophages derived from macaque induced pluripotent stem cells, and mice engrafted with macaque CD34+-enriched hematopoietic stem cells...
April 2024: Emerging Infectious Diseases