Selin Jessa, Alexis Blanchet-Cohen, Brian Krug, Maria Vladoiu, Marie Coutelier, Damien Faury, Brice Poreau, Nicolas De Jay, Steven Hébert, Jean Monlong, W Todd Farmer, Laura K Donovan, Yixing Hu, Melissa K McConechy, Florence M G Cavalli, Leonie G Mikael, Benjamin Ellezam, Maxime Richer, Andréa Allaire, Alexander G Weil, Jeffrey Atkinson, Jean-Pierre Farmer, Roy W R Dudley, Valerie Larouche, Louis Crevier, Steffen Albrecht, Mariella G Filbin, Hervé Sartelet, Pierre-Eric Lutz, Corina Nagy, Gustavo Turecki, Santiago Costantino, Peter B Dirks, Keith K Murai, Guillaume Bourque, Jiannis Ragoussis, Livia Garzia, Michael D Taylor, Nada Jabado, Claudia L Kleinman
Childhood brain tumors have suspected prenatal origins. To identify vulnerable developmental states, we generated a single-cell transcriptome atlas of >65,000 cells from embryonal pons and forebrain, two major tumor locations. We derived signatures for 191 distinct cell populations and defined the regional cellular diversity and differentiation dynamics. Projection of bulk tumor transcriptomes onto this dataset shows that WNT medulloblastomas match the rhombic lip-derived mossy fiber neuronal lineage and embryonal tumors with multilayered rosettes fully recapitulate a neuronal lineage, while group 2a/b atypical teratoid/rhabdoid tumors may originate outside the neuroectoderm...
December 2019: Nature Genetics