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Samiran Bandyopadhyay, Jaydeep Banerjee, Debaraj Bhattacharyya, Indranil Samanta, Achintya Mahanti, Tapan K Dutta, Sarbaswarup Ghosh, Pramod K Nanda, Premanshu Dandapat, Subhasish Bandyopadhyay
We investigated the occurrence of extended-spectrum β-lactamase (ESBL) and AmpC-type β-lactamase (ACBL) producing quinolone-resistant Klebsiella pneumoniae (KP) in milk samples of apparently healthy buffaloes (n = 348) and buffaloes (n = 19) with evidence of subclinical mastitis from seven districts of West Bengal, India. In total, 12 ESBL producing KP were isolated with blaCTX-M-15 gene and 7 of them were ACBL producers, as well. The blaCTX-M-15 genes were carried by transposable element ISEcp1. The plasmid-mediated quinolone resistance genes-qnrS, qnrA, qnrB, qepA, and aac(6')-Ib-cr were detected in five, one, three, four, and one isolate (s), respectively...
March 22, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Doniparthi Venkata Pavan Kumar, Jesinth Mohan, P S Rakesh, Jasmine Prasad, Lenikumar Joseph
Introduction: Neonatal sepsis is a leading cause of neonatal mortality and morbidity in the world. The objective of the current study was to detect the common causative microorganisms of neonatal sepsis and their antimicrobial resistance patterns in a rural secondary hospital in Tamil Nadu, India. Materials and Methods: Neonates (0-28 days) admitted to this newborn care unit from October 2013 to September 2015, with a diagnosis of probable sepsis were studied. All the enrolled babies had blood cultures taken and were followed up till final outcome, which was discharge or death, irrespective of culture result...
October 2017: Journal of Family Medicine and Primary Care
Jiang Wei, Yang Wenjie, Liu Ping, Wang Na, Ren Haixia, Zhao Xuequn
Overuse and misuse of antibiotics leads to rapid evolution of antibiotic-resistant bacteria and antibiotic resistance genes. Klebsiella pneumoniae has become the most common pathogenic bacterium accountable for nosocomial infections due to its high virulence factor and general occurrence of resistance to most antibiotics. The β-lactamase signaling pathway has been suggested to be involved in antibiotic resistance against β-lactams in Klebsiella pneumoniae . In the present study, the molecular mechanism of the antibiotic resistance of Klebsiella pneumoniae was investigated and the results indicated involvement of the β-arrestin recruitment-induced β-lactamase signaling pathway...
March 2018: Experimental and Therapeutic Medicine
Nayeem Ahmad, Shamsi Khalid, Syed M Ali, Asad U Khan
Carbapenem-resistance among enterobacteriaceae has become a global health concern. The objective of this study was to understand NDM producing enterobacteriaceae and their genetic basis of resistance, spreading in neonatal intensive care unit. Carbapenem resistant NDM producing enterobacteriaceae isolates were recovered from rectal swab and blood sample of infants admitted in NICU. These were determined by using Carba-NP test. All isolates were identified using BD PhoenixTM-100 and MICs were determined by broth microdilution method...
2018: Frontiers in Microbiology
Manesh Abi, Shankar Chaitra, Leb Nabarro, M Varghese George, Veeraraghavan Balaji
No abstract text is available yet for this article.
January 2018: Journal of Global Infectious Diseases
Neeraj Kumar Tulara
Urinary tract infection (UTI) is a common and painful human illness that, unfortunately not responsive to commonly used antibiotics in current practice. The role of fosfomycin and nitrofurantoin in the era of growing bacteria resistance has been widely discussed. In this study, we aimed to know the local antimicrobial susceptibilities, fosfomycin and nitrofurantoin susceptibility in particular, for urinary extended-spectrum-beta-lactamase-producing Escherichia coli and Escherichia pneumoniae (ESBL-EC and ESBL-KP) isolates in our hospital...
January 2018: Journal of Global Infectious Diseases
E Yoko Furuya, Bevin Cohen, Haomiao Jia, Elaine L Larson
OBJECTIVE To evaluate the impact of universal contact precautions (UCP) on rates of multidrug-resistant organisms (MDROs) in intensive care units (ICUs) over 9 years DESIGN Retrospective, nonrandomized observational study SETTING An 800-bed adult academic medical center in New York City PARTICIPANTS All patients admitted to 6 ICUs, 3 of which instituted UCP in 2007 METHODS Using a comparative effectiveness approach, we studied the longitudinal impact of UCP on MDRO incidence density rates, including methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and carbapenem-resistant Klebsiella pneumoniae...
March 22, 2018: Infection Control and Hospital Epidemiology
Rika Narashima, Yuya Hasunuma, Yoshikazu Tokuoka
In order to develop a new method to detect β-lactamase, we determined degradation of β-lactam antibiotics, ampicillin sodium (ABPC) and cefotaxime sodium (CTX), by β-lactamase-producing Klebsiella pneumoniae ( K. pneumoniae ) in terms of high performance liquid chromatography (HPLC). Using HPLC with an ODS column and an eluent composed of phosphate buffer and methanol, we could detect ABPC and CTX within 10 min. After cultured with K. pneumoniae , ABPC and CTX were degraded. The degradation rate corresponding to the rate of peak area incubated with and without bacteria increased with increasing McFarland No...
March 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
Karen E Bowker, Alan R Noel, Sharon Tomaselli, Marie Attwood, Alasdair P MacGowan
Background: The pharmacodynamics of inhaled antimicrobials are poorly studied. Amikacin is being developed for inhalational therapy as BAY 41-6551. Objectives: We employed an in vitro pharmacokinetic model to study the pharmacokinetics/pharmacodynamics of amikacin. Methods: A dose-ranging design was used to establish fAUC/MIC and fCmax/MIC targets for static, -1 log drop and -2 log drop effects for strains of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa...
March 19, 2018: Journal of Antimicrobial Chemotherapy
Svetlana Ferreira Lima, Marcela Lucas de Souza Bicalho, Rodrigo Carvalho Bicalho
Amplicon sequencing technique has been increasingly applied to the clinical setting as a sensitive diagnostic tool. Therefore, it is of great importance to develop a DNA extraction method that accurate isolates DNA from complex host-associated microbiota. Given the multifactorial etiology of clinical mastitis and the diversified lifestyle of bacterial species harboring in milk, here four distinct milk sample fractions: raw whole milk, milk fat, casein-pellet, and casein-pellet + fat from healthy cows and cows with clinical mastitis, were subjected to bead-beating DNA extraction, followed by high-throughput sequencing...
2018: PloS One
Irene Galani, Konstantina Nafplioti, Marianthi Chatzikonstantinou, Maria Souli
PURPOSE: The aim of this study was to evaluate the in vitro activity of double-carbapenem combinations against OXA-48-producing Klebsiella pneumoniae clinical isolates. METHODOLOGY: Double combinations of ertapenem, meropenem and imipenem were evaluated for synergy and bactericidal activity using the time-kill methodology. All antibiotics were tested at 10 mg l-1 and at a sub-inhibitory concentration of 0.5× minimum inhibitory concentration (MIC) for isolates with a carbapenem MIC≤8 mg l-1 ...
March 21, 2018: Journal of Medical Microbiology
Nadège Bossuet-Greif, Julien Vignard, Frédéric Taieb, Gladys Mirey, Damien Dubois, Claude Petit, Eric Oswald, Jean-Philippe Nougayrède
Colibactins are hybrid polyketide-nonribosomal peptides produced by Escherichia coli , Klebsiella pneumoniae , and other Enterobacteriaceae harboring the pks genomic island. These genotoxic metabolites are produced by pks -encoded peptide-polyketide synthases as inactive prodrugs called precolibactins, which are then converted to colibactins by deacylation for DNA-damaging effects. Colibactins are bona fide virulence factors and are suspected of promoting colorectal carcinogenesis when produced by intestinal E...
March 20, 2018: MBio
Xingyu Zhang, Di Chen, Guifeng Xu, Weichun Huang, Xing Wang
Infection by carbapenem-resistant Klebsiella pneumoniae (CR-KP) is a public health challenge worldwide, in particular among children, which was associated with high morbidity and mortality rates. There was limited data in pediatric populations, thus this study aimed to investigate molecular epidemiology and drug resistant mechanism of CR-KP strains from pediatric patients in Shanghai, China. A total of 41 clinical CR-KP isolates from sputum, urine, blood or drainage fluid were collected between July 2014 and May 2015 in Shanghai Children's Medical Center...
2018: PloS One
Yanbin Li, Qin Zhang, Li Deng, Zhanwen Liu, Hui Jiang, Fang Wang
A new hydrogen-producing bacterium was isolated from the intestine of wild carp (Cyprinus carpio L.) of the Tarim River Basin. The isolate was identified as Klebsiella sp. based on 16S rDNA gene sequencing and examination of physiological and biochemical characteristics. The isolated strain, Klebsiella sp. WL1316, could effectively produce a high yield of hydrogen by using cotton stalk hydrolysate as substrate. The optimum fermentation conditions for hydrogen production were determined as follows: an initial sugar concentration of 40 g/L, a fermentation temperature of 37 °C and an initial pH value of 8...
March 19, 2018: Applied Microbiology and Biotechnology
Ram Chandra, Vineet Kumar, Sonam Tripathi
The extracted sugarcane molasses-melanoidins showed the presence of Mn (8.20), Cr (2.97), Zn (16.61), Cu (2.55), Fe (373.95), Pb (2.59), and Ni (4.18 mg L-1 ) along with mixture of other organic compounds which have endocrine-disrupting chemicals (EDCs) properties. A consortium of aerobic bacteria comprising Klebsiella pneumoniae (KU321273), Salmonella enteric (KU726954), Enterobacter aerogenes (KU726955), and Enterobacter cloacae (KU726957) showed the optimum decolourisation of molasses-melanoidins up to 81% through co-metabolism in the presence of glucose (1...
April 2018: 3 Biotech
Nikos Ulrich, Petra Gastmeier, Ralf-Peter Vonberg
Background: Identifying the source of an outbreak is the most crucial aspect of any outbreak investigation. In this review, we address the frequently discussed question of whether (rectal) screening of health care workers (HCWs) should be carried out when dealing with outbreaks caused by gram negative bacteria (GNB).A systematic search of the medical literature was performed, including the Worldwide Outbreak Database and PubMed. Outbreaks got included if a HCW was the source of the outbreak and the causative pathogen was an Escherichia coli , Klebsiella spp ...
2018: Antimicrobial Resistance and Infection Control
Dragos Ciocan, Vinciane Rebours, Cosmin Sebastian Voican, Laura Wrzosek, Virginie Puchois, Anne-Marie Cassard, Gabriel Perlemuter
Excessive alcohol consumption leads to severe alcoholic hepatitis (sAH) or chronic alcoholic pancreatitis (CAP) only in a subset of patients. We aimed to characterize the intestinal microbiota profiles of alcoholic patients according to the presence and nature of the complications observed: sAH or CAP. Eighty two alcoholic patients were included according to their complications: CAP (N = 24), sAH (N = 13) or no complications (alcoholic controls, AC, N = 45). We analyzed the intestinal microbiota by high-throughput sequencing...
March 19, 2018: Scientific Reports
Alessandro Russo, Simone Giuliano, Giancarlo Ceccarelli, Francesco Alessandri, Alessandra Giordano, Grazia Brunetti, Mario Venditti
Objectives: a significant cause of mortality in intensive care unit (ICU) is represented by multidrug-resistant (MDR) gram-negative bacteria, such as MDR Acinetobacter baumannii (MDR-AB) and Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae (KPC-Kp). Aim of the present study was the comparison of clinical features, therapy and outcome of patients who developed septic shock due to either MDR-AB or KPC-Kp. Methods: were retrospectively analyzed patients admitted to the intensive care unit (ICU) of a teaching hospital, from November 2010 to December 2015, who developed septic shock due to MDR-AB or KPC-Kp infection...
March 19, 2018: Antimicrobial Agents and Chemotherapy
Mengyuan Qi, Bin Liang, Rongrong Chen, Xun Sun, Zhiling Li, Xiaodan Ma, Youkang Zhao, Deyong Kong, Jun Wang, Aijie Wang
The bioelectrotransformation efficiency of various organic matters and corresponding electrode biofilm community formation as well as electron transfer efficiency in bioelectrochemical systems (BESs) with different modified electrodes has been extensively studied on the anode side. However, the effects of cathode interface characteristics towards the BESs bioelectrotransformation performance remain poorly understood. In this study, the nitrobenzene-reducing biocathode catalytic efficiency and community structure in response to different modified electrodes (control: hydrophobic and no charge; -SH: hydrophobic and single negative charge; -NH2 : hydrophilic and single positive charge -NH-NH2 : hydrophilic and double positive charges) were investigated...
March 13, 2018: Chemosphere
Isaac Turner, Kiran V Garimella, Zamin Iqbal, Gil McVean
Motivation: The de Bruijn graph is a simple and efficient data structure that is used in many areas of sequence analysis including genome assembly, read error correction and variant calling. The data structure has a single parameter k, is straightforward to implement and is tractable for large genomes with high sequencing depth. It also enables representation of multiple samples simultaneously to facilitate comparison. However, unlike the string graph, a de Bruijn graph does not retain long range information that is inherent in the read data...
March 15, 2018: Bioinformatics
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