keyword
MENU ▼
Read by QxMD icon Read
search

CCM

keyword
https://www.readbyqxmd.com/read/29155332/unusual-features-of-the-large-linear-plasmid-psa3239-from-streptomyces-aureofaciens-ccm-3239
#1
Erik Mingyar, Renata Novakova, Renata Knirschova, Lubomira Feckova, Carmen Bekeova, Jan Kormanec
We previously identified the aur1 gene cluster, responsible for the production of the angucycline antibiotic auricin in Streptomyces aureofaciens CCM 3239. Pulse-field gel electrophoresis showed a single, 241kb linear plasmid, pSA3239, in this strain, and several approaches confirmed the presence of the aur1 cluster in this plasmid. We report here the nucleotide sequence of this 241,076-bp plasmid. pSA3239 contains an unprecedentedly small (13bp) telomeric sequence CCCGCGGAGCGGG, which is identical to the conserved Palindrome I sequence involved in the priming of end-patching replication...
November 16, 2017: Gene
https://www.readbyqxmd.com/read/29143212/rhodococcus-psychrotolerans-sp-nov-isolated-from-rhizosphere-of-deschampsia-antarctica
#2
Leonardo Jose Silva, Danilo Tosta Souza, Diego Bonaldo Genuario, Harold Alexander Vargas Hoyos, Suikinai Nobre Santos, Luiz Henrique Rosa, Tiago Domingues Zucchi, Itamar Soares Melo
A novel actinobacterium, designated strain CMAA 1533(T), was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533(T) was found to grow over a wide range of temperatures (4-28 °C) and pH (4-10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533(T) belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus...
November 15, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29139540/neurosphere-formation-potential-resides-not-in-the-caudal-cell-mass-but-in-the-secondary-neural-tube
#3
Ji Yeoun Lee, Eun Sun Lee, Saet Pyoul Kim, Myung-Sook Lee, Ji Hoon Phi, Seung-Ki Kim, Young-Il Hwang, Kyu-Chang Wang
The caudal cell mass (CCM) is known as the main player in secondary neurulation, forming the secondary neural tube (2NT). This suggests that the CCM may have the character of neural progenitor cells. The neural potential of the CCM and the 2NT (CCM + 2NT) was assessed by in vitro culture of neurospheres during Hamburger and Hamilton stages (HH) of secondary neurulation (HH16 to HH32). We also analyzed the neural potential of the developing central nervous system (CNS) by comparing the neurosphere culture from the brain, upper / lower spinal cord, and CCM + 2NT from various HH stages...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/29137672/the-primary-health-care-in-the-emirate-of-abu-dhabi-are-they-aligned-with-the-chronic-care-model-elements
#4
Marília Silva Paulo, Tom Loney, Luís Velez Lapão
BACKGROUND: Abu Dhabi is the capital of the United Arab Emirates (UAE) and the largest of the seven emirates in terms of land mass and population. Abu Dhabi emirate has three different geographical regions: the Central Capital District, the Eastern Region, and the Western Region. The health system has been regulated by the Health Authority - Abu Dhabi (HAAD), and has been provided by the Abu Dhabi Health Services Company (SEHA), since 2007. The UAE has a high population-burden of morbidity and mortality related to chronic diseases...
November 14, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/29131301/genome-wide-identification-of-tolerance-mechanisms-towards-p-coumaric-acid-in-pseudomonas-putida
#5
Patricia Calero, Sheila I Jensen, Klara Bojanovič, Rebecca Lennen, Anna Koza, Alex T Nielsen
The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 towards eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant towards three of the eleven compounds when compared to Escherichia coli. Increased tolerance was for example found towards p-coumaric acid, an interesting precursor for polymerization with a significant industrial relevance...
November 13, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29127620/potential-of-the-strain-raoultella-sp-kdf8-for-removal-of-analgesics
#6
Andrea Palyzová, Jiří Zahradník, Helena Marešová, Lucie Sokolová, Eva Kyslíková, Michal Grulich, Václav Štěpánek, Tomáš Řezanka, Pavel Kyslík
The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited in the Czech Collection of Microorganisms under the number CCM 8678. A growing culture efficiently removed diclofenac (92% removal) and partially also codeine (about 30% degradation) from culture supernatants within 72 h at 28 °C...
November 11, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29125512/a-chronic-care-model-for-spina-bifida-transition
#7
Ellen Fremion, Melissa Morrison-Jacobus, Jonathan Castillo, Heidi Castillo, Kathryn Ostermaier
Providing comprehensive transition care for adolescents and young adults with spina bifida (AYASB) requires a structured approach to addressing chronic condition management, self-management, care coordination, and health care navigation that is adaptable to the various levels of cognitive ability, physical function, and family/community environments within the population. This commentary (1) highlights AYASB transition program needs identified in the literature and within a local community, (2) analyzes advantages and limitations of published AYASB transition care models in addressing these needs, (3) demonstrates how a SB transition clinic used the Chronic Care Model (CCM) to develop a comprehensive AYASB transition program, and (4) examines the potential feasibility in adapting this model to other SB clinics...
October 20, 2017: Journal of Pediatric Rehabilitation Medicine
https://www.readbyqxmd.com/read/29120011/four-year-long-term-follow-up-of-diabetes-patients-after-implementation-of-the-chronic-care-model-in-primary-care-a-cross-sectional-study
#8
Corinne Chmiel, Irina Giewer, Anja Frei, Thomas Rosemann
AIMS: Implementing the Chronic Care Model (CCM) via involvement of specially trained practice nurses improves cardiovascular risk profiles and perception of care among type 2 diabetes patients in small primary care practices (PCPs) in the short term. Little is known about the long-term effects of this intervention. METHODS: Cross-sectional survey among the participants of the cluster randomised controlled CARAT trial (30 PCPs, 303 diabetes patients), 3 years after its completion...
November 9, 2017: Swiss Medical Weekly
https://www.readbyqxmd.com/read/29116040/fictibacillus-aquaticus-sp-nov-isolated-from-downstream-river-water
#9
Deepika Pal, Ayanka Bhardwaj, Navjot Kaur, Sarabjeet Kour Sudan, Bhawana Bisht, Munesh Kumari, Bhawna Vyas, Srinivasan Krishnamurthi, Shanmugam Mayilraj
A Gram-stain-positive, facultatively anaerobic bacterial strain, GDSW-R2A3(T), was isolated from a downstream water sample collected from the river Ganges, India. Analysis of the 16S rRNA gene sequence of strain GDSW-R2A3(T) revealed its affiliation to the family Bacillaceae. Further analysis using a polyphasic approach revealed that strain GDSW-R2A3(T) was most closely related to the genus Fictibacillus. Analysis of the almost-complete (1488 bp) 16S rRNA gene sequence of strain GDSW-R2A3(T) revealed the highest level of sequence similarity with Fictibacillus phosphorivorans CCM 8426(T) (98...
November 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29100780/amphiphilic-derivatives-of-3%C3%AE-17%C3%AE-3-hydroxyandrost-5-ene-17-carboxylic-acid
#10
Zülal Özdemir, Uladzimir Bildziukevich, David Šaman, Libor Havlíček, Lucie Rárová, Lucie Navrátilová, Zdeněk Wimmer
A series of amphiphilic derivatives of (3β,17β)-3-hydroxyandrost-5-ene-17-carboxylic acid (1) with the polyamine spermine and three other diamines, 1,2-diaminoethane, piperazine and cadaverine, were synthesized and their antimicrobial activity and cytotoxicity were investigated. Among the target compounds, several ones showed antimicrobial activity on Gram positive and Gram negative microorganisms. The most active compounds were 20 (Streptococcus mutans CCM 7409, 3.125 µM), 16 (Streptococcus mutans CCM 7409, 12...
October 31, 2017: Steroids
https://www.readbyqxmd.com/read/29096714/isolation-and-prolonged-expansion-of-oral-mesenchymal-stem-cells-under-clinical-grade-gmp-compliant-conditions-differentially-affects-stemness-properties
#11
Athina Bakopoulou, Danae Apatzidou, Eleni Aggelidou, Evangelia Gousopoulou, Gabriele Leyhausen, Joachim Volk, Aristeidis Kritis, Petros Koidis, Werner Geurtsen
BACKGROUND: Development of clinical-grade cell preparations is central to meeting the regulatory requirements for cellular therapies under good manufacturing practice-compliant (cGMP) conditions. Since addition of animal serum in culture media may compromise safe and efficient expansion of mesenchymal stem cells (MSCs) for clinical use, this study aimed to investigate the potential of two serum/xeno-free, cGMP culture systems to maintain long-term "stemness" of oral MSCs (dental pulp stem cells (DPSCs) and alveolar bone marrow MSCs (aBMMSCs)), compared to conventional serum-based expansion...
November 2, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29090935/discovery-of-the-membrane-binding-domain-in-trifunctional-proline-utilization-a
#12
Shelbi Christgen, Weidong Zhu, Nikhilesh Sanyal, Bushra Mariam Bibi, John J Tanner, Donald Frederick Becker
Escherichia coli proline utilization A (EcPutA) is the archetype of trifunctional PutA flavoproteins, which function both as regulators of the proline utilization operon and bifunctional enzymes that catalyze the four-electron oxidation of proline to glutamate. EcPutA shifts from a self-regulating transcriptional repressor to a bifunctional enzyme in a process known as functional switching. The flavin redox state dictates the function EcPutA. Upon proline oxidation, the flavin becomes reduced, triggering a conformational change that causes EcPutA to dissociate from the put regulon and bind to the cellular membrane...
November 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/29079617/staphylococcus-edaphicus-sp-nov-isolated-in-antarctica-harbours-mecc-gene-and-genomic-islands-with-suspected-role-in-adaptation-to-extreme-environment
#13
Roman Pantůček, Ivo Sedláček, Adéla Indráková, Veronika Vrbovská, Ivana Mašlaňová, Vojtěch Kovařovic, Pavel Švec, Stanislava Králová, Lucie Krištofová, Jana Kekláková, Petr Petráš, Jiří Doškař
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources, stone fragments and sandy soil in James Ross Island, Antarctica. Here we describe properties of novel species of the genus Staphylococcus that has near identical 16S rRNA gene sequence to Staphylococcus saprophyticus However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at whole genome level, average nucleotide identity <85 %, and inferred DNA-DNA hybridization <30%...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29077965/phosphorus-bioavailability-in-increased-protein-reduced-fiber-canola-meal-conventional-canola-meal-and-soybean-meal-fed-to-crossbred-chicks
#14
C D Hanna, C K Foran, P L Utterback, H H Stein, C M Parsons
Three experiments were conducted to evaluate 3 increased-protein, reduced-fiber canola meals (CM) (CMA, CMB, and Test CM), 2 conventional CM (CCM), and 2 soybean meals (SBM). For determination of P bioavailability in CM and SBM, a P-deficient cornstarch-dextrose-SBM basal diet was fed as Diet 1. The latter basal diet was then supplemented with 0.05 and 0.10% P from KH2PO4 or 12.5 and 25% of a CM or SBM. In addition, the effect of phytase enzyme on bioavailability of the P in CMA, Test CM, and one of the CCM was determined using P-deficient 45% CM-cornstarch-dextrose diets (0...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29058658/winogradskyella-haliclonae-sp-nov-isolated-from-a-marine-sponge-of-the-genus-haliclona
#15
Johannes Schellenberg, Hans-Jürgen Busse, Martin Hardt, Patrick Schubert, Thomas Wilke, Peter Kämpfer, Stefanie P Glaeser
A yellow-pigmented, Gram-stain-negative, motile and rod-shaped bacterium, strain M1A16(T), was isolated from the internal tissue of a sponge of the genus Haliclona, which was long-term cultured in the CEMarin aquaria system at Justus Liebig University of Giessen. The strain grew well at 20-32 °C (optimum 25 °C), in the presence of 0-6 % NaCl (optimum 3 %), and at pH 5.5-9.0 (optimum pH 7.0-8.0). Phylogenetic analysis based on its 16S rRNA gene sequence placed the strain within the monophyletic cluster of the genus Winogradskyella with highest sequence similarity to Winogradskyella jejuensis CP32(T) (98...
October 23, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29051912/evaluating-diabetes-care-for-patients-with-serious-mental-illness-using-the-chronic-care-model-a-pilot-study
#16
Kelly Vaez, Lauren Diegel-Vacek, Catherine Ryan, Pamela Martyn-Nemeth
People with serious mental illness (SMI) have a higher incidence of type 2 diabetes mellitus (T2DM) and shorter life span due to medical health problems. The chronic care model (CCM) has been used to improve care of patients with T2DM. One clinical organization that provided primary care to patients with SMI had excellent diabetes outcomes but did not have information on how they achieved those outcomes. Thus, we conducted a pilot study chart review for 30 patients with T2DM and SMI to determine how well the clinic's system aligned with the overall CCM components and which components correlated with diabetes control...
January 2017: Health Services Research and Managerial Epidemiology
https://www.readbyqxmd.com/read/29046973/clinical-management-of-cavernous-malformations
#17
REVIEW
Kelly D Flemming
PURPOSE OF REVIEW: This study aims to review the current epidemiology and clinical management of patients with cavernous malformations (CM). RECENT FINDINGS: Hemorrhage is the most feared complication and leads to morbidity in patients with CM. Multiple studies including three meta-analyses have provided useful estimates of hemorrhage risk, but have failed to identify a modifiable risk factor for prevention of cavernous malformation related hemorrhage. In treating the CM itself, surgical risk is weighed against the natural history...
October 18, 2017: Current Cardiology Reports
https://www.readbyqxmd.com/read/29044354/protocol-for-the-evaluation-of-a-chronic-care-model-experience-in-rome
#18
D Mipatrini, A Sinopoli, C Sestili, M Di Marcoberardino, P Giuliani, G Grasso, A Lancia, E Megli, R Mete, M G Pennafina, M Pirrò, S Tartaglia, F Vero, G La Torre
INTRODUCTION: Chronic diseases are the leading cause of death and disability in almost all over the world; in Europe causing over 9 million deaths per year according to WHO estimates. A promising health organization model for chronic disease management is represented by the Chronic Care Model (CCM). In the 12th district of the ASL Roma 2 since 4 years was implemented a CCM for the management of patients affected by diabetes and/or at high cardiovascular risk. OBJECTIVE: Aim of this study is to evaluate the effectiveness of the Chronic Care Model (CCM) for the management of chronic disease in terms of mortality reduction, avoidable hospitalizations reduction and improvement of clinical parameters...
September 2017: La Clinica Terapeutica
https://www.readbyqxmd.com/read/29031980/quality-by-design-qbd-approach-to-optimize-the-formulation-of-a-bilayer-combination-tablet-telmiduo-%C3%A2-manufactured-via-high-shear-wet-granulation
#19
Ah Ram Lee, Seok Young Kwon, Du Hyung Choi, Eun Seok Park
A bilayer tablet, which consisted of telmisartan and amlodipine besylate, was formulated based on a Quality by Design (QbD) approach. The control and response factors were determined based on primary knowledge and the target values of the control tablet (Twynsta(®)). A D-optimal mixture design was used to obtain the optimal formulations in terms of D-mannitol, crospovidone, and MCC for the telmisartan layer, and CCM-Na, PVP K25, and Prosolv for the amlodipine layer. The quantitative effects of the different formulation factors on the response factors were accurately predicted using the equations of best fit and a strong linearity was observed between the predicted and actual values of the response factors...
October 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29031933/relevant-models-and-elements-of-integrated-care-for-multi-morbidity-results-of-a-scoping-review
#20
Verena Struckmann, Fenna R M Leijten, Ewout van Ginneken, Markus Kraus, Miriam Reiss, Anne Spranger, Melinde R S Boland, Thomas Czypionka, Reinhard Busse, Maureen Rutten-van Mölken
BACKGROUND: In order to provide adequate care for the growing group of persons with multi-morbidity, innovative integrated care programmes are appearing. The aims of the current scoping review were to i) identify relevant models and elements of integrated care for multi-morbidity and ii) to subsequently identify which of these models and elements are applied in integrated care programmes for multi-morbidity. METHODS: A scoping review was conducted in the following scientific databases: Cochrane, Embase, PubMed, PsycInfo, Scopus, Sociological Abstracts, Social Services Abstracts, and Web of Science...
October 6, 2017: Health Policy
keyword
keyword
16898
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"