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https://www.readbyqxmd.com/read/30110560/microrna-204-5p-suppresses-il6-mediated-inflammatory-response-and-chemokine-generation-in-hk-2-renal-tubular-epithelial-cells-by-targeting-il6r
#1
Hua Li, Jibo Wang, Xiaoru Liu, Qiang Cheng
During the pathogenetic process of varied kidney diseases, renal tubules are the major sites in response to detrimental insults, including pro-inflammatory stimuli. MicroRNA-204-5p (miR-204-5p) can be detected in the renal tubular epithelial cells in the normal kidney, its expression however is downregulated in the kidney with pathological changes. This study aimed to investigate the role of miR-204-5p in interleukin 6 (IL6)-mediated inflammatory response and chemokine production in HK-2 renal tubular cells...
August 15, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/30098377/nlrp3-deficiency-ameliorates-renal-inflammation-and-fibrosis-in-diabetic-mice
#2
Ming Wu, Weixia Han, Shan Song, Yunxia Du, Chao Liu, Nan Chen, Haijiang Wu, Yonghong Shi, Huijun Duan
Diabetic nephropathy (DN) is the leading cause of end-stage renal disease. Activation of the nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome has been reported in diabetic kidney, yet the potential role of NLRP3 inflammasome in DN is not well known. In this study, we explored the role of NLRP3 inflammasome on inflammation and fibrosis in diabetic kidney using NLRP3 knockout mice. Renal expression of NLRP3, caspase-1 p10, interleukin-18 (IL-18) and cleaved IL-1β was increased in diabetic wild-type (WT) mice at 24 weeks...
August 8, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/30093705/fenbendazole-acts-as-a-moderate-microtubule-destabilizing-agent-and-causes-cancer-cell-death-by-modulating-multiple-cellular-pathways
#3
Nilambra Dogra, Ashok Kumar, Tapas Mukhopadhyay
Drugs that are already clinically approved or experimentally tested for conditions other than cancer, but are found to possess previously unrecognized cytotoxicity towards malignant cells, may serve as fitting anti-cancer candidates. Methyl N-(6-phenylsulfanyl-1H benzimidazol-2-yl) carbamate [Fenbendazole, FZ], a benzimidazole compound, is a safe and inexpensive anthelmintic drug possessing an efficient anti-proliferative activity. In our earlier work, we reported a potent growth-inhibitory activity of FZ caused partially by impairment of proteasomal function...
August 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30081092/the-tgf%C3%AE-erk-pathway-contributes-to-notch3-upregulation-in-the-renal-tubular-epithelial-cells-of-patients-with-obstructive-nephropathy
#4
Mei Huang, Jin Zhang, Hui Xu, Ting Ding, Damu Tang, Qiongjing Yuan, Lijian Tao, Zunlong Ye
Renal interstitial fibrosis is a common renal injury resulted from a variety of chronic kidney conditions and an array of factors. We report here that Notch3 is a potential contributor. In comparison to 6 healthy individuals, a robust elevation of Notch3 expression was observed in the renal tubular epithelial cells of 18 patients with obstructive nephropathy. In a rat unilateral ureteral obstruction (UUO) model which mimics the human disease, Notch3 upregulation closely followed the course of renal injury, renal fibrosis, TGFβ expression, and alpha-smooth muscle actin (α-SMA) expression, suggesting a role of Notch3 in promoting tubulointerstitial fibrosis...
August 3, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/30072956/combining-sglt2-inhibition-with-a-thiazolidinedione-additively-attenuate-the-very-early-phase-of-diabetic-nephropathy-progression-in-type-2-diabetes-mellitus
#5
Eugene Han, Eugene Shin, Gyuri Kim, Ji-Yeon Lee, Yong-Ho Lee, Byung-Wan Lee, Eun Seok Kang, Bong-Soo Cha
Although both sodium glucose co-transporter 2 inhibition by dapagliflozin and thiazolidinedione, pioglitazone have glucose-lowering and anti-inflammatory effects, the therapeutic efficacy of their combination on diabetic nephropathy has not been investigated. 9-week-old male db/db mice were randomly assigned to 4 groups and administrated with (1) vehicle, (2) dapagliflozin, (3) pioglitazone, or (4) dapagliflozin and pioglitazone combination. Human proximal tubule (HK-2) cells were treated with glucose or palmitate acid in the presence of medium, dapagliflozin, pioglitazone, or both...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/30066965/preparation-and-evaluation-of-anti-renal-fibrosis-activity-of-novel-truncated-tgf-%C3%AE-receptor-type-ii
#6
Haifeng Liu, Zhongmin Zhang, Yuting Li, Xiaoli Wang, Yufei Zhang, Yanhui Chu, Xiaohuan Yuan, Xiaohua Wang
Production of excessive transforming growth factor-beta 1 (TGF-β1) with elevated TGF-β1 activity has been implicated in renal fibrosis via renal epithelial cells activation and collagen deposition. As such, attenuating the binding of TGF-β1 to its receptor transforming growth factor-beta receptor type II (TGF-βRII) in TGF-β1-dependent signaling is an attractive target for the control of renal fibrosis. Here, we verified the interaction between novel truncated human TGF-βRII (thTβRII, Thr23-Gln166) and TGF-β1, prepared thTβRII in Escherichia coli, and assessed the effects of thTβRII on TGF-β1-induced human kidney epithelial cells (HK-2) and unilateral ureteral obstruction (UUO) model of renal fibrosis...
August 1, 2018: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/30062051/autophagy-activation-contributes-to-lipid-accumulation-in-tubular-epithelial-cells-during-kidney-fibrosis
#7
Qi Yan, Yuan Song, Lu Zhang, Zhaowei Chen, Cheng Yang, Shan Liu, Xiaohan Yuan, Hongyu Gao, Guohua Ding, Huiming Wang
Sustained activation of autophagy and lipid accumulation in tubular epithelial cells (TECs) are both associated with the kidney fibrosis progression. Autophagy has been found involved in the lipid metabolism regulation through a bi-directional mechanism of inducing lipolysis as well as promoting lipid droplet formation. However, whether and how autophagy influences lipid accumulation in kidney fibrosis remain unclear. In the current study, we show that UUO-induced lipid accumulation in tubular cells was significantly reduced when the pharmacological inhibitor 3-MA or CQ was administrated both in vivo and in vitro...
2018: Cell Death Discovery
https://www.readbyqxmd.com/read/30061948/comparison-of-exosomal-micrornas-secreted-by-786-o-clear-cell-renal-carcinoma-cells-and-hk-2-proximal-tubule-derived-cells-in-culture-identifies-microrna-205-as-a-potential-biomarker-of-clear-cell-renal-carcinoma
#8
Victor C Crentsil, Hui Liu, Donald F Sellitti
Previous reports have indicated that the abundance of specific microRNAs (miRNA) contained within the exosome/microvesicle compartment of patient biofluids may be useful in diagnosing specific types of cancer. In the present study, the 786-O cell line, which is derived from a clear cell renal cell carcinoma (ccRCC), was used as an in vitro ccRCC tumor model and the human renal proximal tubule cell line HK-2 was used as its normal renal tissue control to investigate the similarities of exosomal content of selected ccRCC miRNA biomarkers in the supernatant with the content of those markers in the cells themselves...
July 2018: Oncology Letters
https://www.readbyqxmd.com/read/30061164/hexokinase-2-as-a-novel-selective-metabolic-target-for-rheumatoid-arthritis
#9
Marta F Bustamante, Patricia G Oliveira, Ricard Garcia-Carbonell, Adam P Croft, Jeff M Smith, Ramon L Serrano, Elsa Sanchez-Lopez, Xiao Liu, Tatiana Kisseleva, Nissim Hay, Christopher D Buckley, Gary S Firestein, Anne N Murphy, Shigeki Miyamoto, Monica Guma
OBJECTIVES: Recent studies indicate that glucose metabolism is altered in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS). Hexokinases (HKs) catalyse the first step in glucose metabolism, and HK2 constitutes the principal HK inducible isoform. We hypothesise that HK2 contributes to the synovial lining hypertrophy and plays a critical role in bone and cartilage damage. METHODS: HK1 and HK2 expression were determined in RA and osteoarthritis (OA) synovial tissue by immunohistochemistry...
July 30, 2018: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/30057316/pioglitazone-induces-hypoxia-inducible-factor-1-activation-in-human-renal-proximal-tubular-epithelial-cell-line-hk-2
#10
Yumiko Urakami-Takebayashi, Yukimi Kuroda, Tadashi Murata, Makoto Miyazaki, Junya Nagai
We previously reported that fatty acid-bearing albumin but not fatty acid-depleted albumin induces hypoxia-inducible factor-1 (HIF-1) activation in human renal proximal tubular epithelial cell line HK-2. Then, an increase in mRNA expression of peroxisome proliferator-activated receptor gamma (PPARγ) was observed on treatment with fatty acid-bearing albumin but not fatty acid-depleted albumin. The aim of this study was to determine whether a PPARγ agonist, pioglitazone, induces HIF-1 activation or not. Treatment with pioglitazone induced HIF-1α mRNA as well as PPARγ mRNA expression in a concentration dependent manner...
July 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30054990/acetyl-11-keto-%C3%AE-boswellic-acid-ameliorates-renal-interstitial-fibrosis-via-klotho-tgf-%C3%AE-smad-signalling-pathway
#11
Minna Liu, Tianlong Liu, Peijin Shang, Yikai Zhang, Limin Liu, Ting Liu, Shiren Sun
Acetyl-11-keto-β-boswellic acid (AKBA), an active triterpenoid compound from the extract of Boswellia serrate, has been reported previously in our group to alleviate fibrosis in vascular remodelling. This study aimed to elucidate the in vivo and in vitro efficacy and mechanism of AKBA in renal interstitial fibrosis. The experimental renal fibrosis was produced in C57BL/6 mice via unilateral ureteral obstruction (UUO). Hypoxia-induced HK-2 cells were used to imitate the pathological process of renal fibrosis in vitro...
July 28, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/30054507/histone-deacetylase-8-protects-human-proximal-tubular-epithelial-cells-from-hypoxia-mimetic-cobalt-and-hypoxia-reoxygenation-induced-mitochondrial-fission-and-cytotoxicity
#12
Soon-Duck Ha, Ori Solomon, Masoud Akbari, Alp Sener, Sung Ouk Kim
Cell death by hypoxia followed by reoxygenation (H/R) is responsible for tissue injury in multiple pathological conditions. Recent studies found that epigenetic reprogramming mediated by histone deacetylases (HDACs) is implicated in H/R-induced cell death. However, among 18 different isoforms comprising 4 classes (I-IV), the role of each HDAC in cell death is largely unknown. This study examined the role of HDAC8, which is the most distinct isoform of class I, in the hypoxia mimetic cobalt- and H/R-induced cytotoxicity of human proximal tubular HK-2 cells...
July 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30041725/self-assembled-honokiol-loaded-microbubbles-in-the-treatment-of-ovarian-cancer-by-ultrasound-irradiation
#13
Qiong Wu, Meiqin Zhang, Hong Luo, Tao Yi
To observe the growth inhibition of subcutaneous ovarian cancer xenografts in nude mice and mechanisms by ultrasound irradiation with honokiol-loaded poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) microbubbles (HK-PCEC-MB) as a promising therapeutic approach to ovarian tumor. HK-PCEC-MB were prepared using the double-emulsion solvent evaporation technique. Particles were characterized with regard to shape, size, distribution and surface potential using dynamic light scattering and the Malvern Zetasizer ZS90 system...
October 1, 2018: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/30038056/-trichosanthes-kirilowii-lectin-alleviates-diabetic-nephropathy-by-inhibiting-the-lox1-nf-%C3%AE%C2%BAb-caspase-9-signaling-pathway
#14
Jiandong Lu, Jinting Peng, Min Xiang, Liangping He, Dongcai Wang, Guoliang Xiong, Shunmin Li
Trichosanthes kirilowii lectin (TKL) has been reported to exert hypoglycemic effects in alloxan-induced diabetic mice. However, there is no evidence showing that its helps to prevent diabetic nephropathy (DN). We used a high glucose (HG)-induced HK-2 cell model and a streptozocin (STZ)-induced Wistar rat model to investigate the effects of TKL on DN, as well as the mechanisms for those effects. Our results showed that TKL significantly increased the viability of HG-treated HK-2 cells and inhibited cell apoptosis...
July 23, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/30037846/toll-like-receptor-4-induced-glycolytic-burst-in-human-monocyte-derived-dendritic-cells-results-from-p38-dependent-stabilization-of-hif-1%C3%AE-and-increased-hexokinase-ii-expression
#15
Laure Perrin-Cocon, Anne Aublin-Gex, Olivier Diaz, Christophe Ramière, Francesco Peri, Patrice André, Vincent Lotteau
Cell metabolism now appears as an essential regulator of immune cells activation. In particular, TLR stimulation triggers metabolic reprogramming of dendritic cells (DCs) with an increased glycolytic flux, whereas inhibition of glycolysis alters their functional activation. The molecular mechanisms involved in the control of glycolysis upon TLR stimulation are poorly understood for human DCs. TLR4 activation of human monocyte-derived DCs (MoDCs) stimulated glycolysis with an increased glucose consumption and lactate production...
July 23, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/30029111/silica-a-trace-geogenic-element-with-emerging-nephrotoxic-potential
#16
Starlaine Mascarenhas, Srikanth Mutnuri, Anasuya Ganguly
Silica is a trace-geogenic compound with limited-bioavailability. It inflicts health-perils like pulmonary-silicosis and chronic kidney disease (CKD), when available via anthropogenic-disturbances. Amidst silica-imposed pathologies, pulmonary toxicological-mechanisms are well-described, ignoring the renal-pathophysiological mechanisms. Hence, the present-study aimed to elucidate cellular-cum-molecular toxicological-mechanisms underlying silica-induced renal-pathology in-vitro. Various toxicity-assessments were used to study effects of silica on the physiological-functions of HK-cells (human-kidney proximal-tubular cells - the toxin's prime target) on chronic (1-7 days) sub-toxic (80 mg/L) and toxic (100-120 mg/L) dosing...
July 16, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30026885/percutaneous-cryoablation-for-stage-t1b-renal-cell-carcinoma-in-a-patient-with-horseshoe-kidney
#17
Kanichiro Shimizu, Hisayo Furube, Kenkichi Michimoto, Takafumi Yanagisawa, Jun Miki, Koichi Kishimoto, Shunichi Sadaoka
We report the first case of percutaneous cryoablation (PCA) for stage T1b renal cell carcinoma (RCC) in a horseshoe kidney (HK). A 76-year-old man with an HK underwent computed tomography-guided PCA for RCC measuring 42 mm (stage T1b) in diameter. Although transcatheter embolization before the PCA and hydrodissection were required to avoid complication and incomplete ablation, PCA was successfully performed without complication. The complete ablation was confirmed on computed tomography images 1 month after the procedure...
June 2018: Radiology Case Reports
https://www.readbyqxmd.com/read/30024615/mir-192-prevents-renal-tubulointerstitial-fibrosis-in-diabetic-nephropathy-by-targeting-egr1
#18
F Liu, Z-P Zhang, G-D Xin, L-H Guo, Q Jiang, Z-X Wang
OBJECTIVE: Diabetic nephropathy (DN), as the most common and serious diabetic microvascular complication, has become the first cause of end-stage renal disease (ESRD) in many countries and regions. However, the pathogenesis of renal fibrosis during the development of DN remains unknown. MATERIALS AND METHODS: The expression levels of miR-192 and early growth response factor 1 (Egr1) were determined by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blotting in the renal tissues of Otsuka-Long-Evans-Tokushima-Fatty (OLETF) and Long-Evans-Tokushima-Otsuka (LETO) rats...
July 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/30023760/effect-of-crystal-shape-and-aggregation-of-calcium-oxalate-monohydrate-on-cellular-toxicity-in-renal-epithelial-cells
#19
Xin-Yuan Sun, Meng Xu, Jian-Ming Ouyang
Renal epithelial cell injury is a key step in inducing kidney stone formation. This injury induced by crystallites with different shapes and aggregation states has been receiving minimal research attention. To compare the shape and aggregation effects of calcium oxalate crystals on their toxicity, we prepared calcium oxalate monohydrate (COM) crystals with the morphology of a hexagonal lozenge, a thin hexagonal lozenge, and their corresponding aggregates. We then compared their toxicities toward human kidney proximal tubular epithelial (HK-2) cells...
September 30, 2017: ACS Omega
https://www.readbyqxmd.com/read/30021352/oncogenic-mir-663a-is-associated-with-cellular-function-and-poor-prognosis-in-renal-cell-carcinoma
#20
Liang Zhou, Xiang Pan, Zuwei Li, Peijie Chen, Jing Quan, Canbin Lin, Yulin Lai, Jinling Xu, Weijie Xu, Xin Guan, Hang Li, Yaoting Gui, Yongqin Lai
BACKGROUND: MicroRNA(miRNA) plays a key regulatory role in various stages of tumorigenesis, including cell growth, cell cycle control, apoptosis avoidance, tissue invasion, and metastasis. Several microRNAs are involved in the development of renal cell carcinoma (RCC) and the malignant transformation process. However, the effects of miR-663a on RCC have rarely been reported. METHODS: In the present study, the expression of miR-663a was examined in RCC using matched normal kidney tissues and four cell lines (786-O, Caki-1, ACHN and HK-2)...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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