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https://www.readbyqxmd.com/read/30181126/analysis-of-pilin-antigenic-variation-in-neisseria-meningitidis-by-next-generation-sequencing
#1
Jing Xu, H Steven Seifert
Many pathogenic microbes evade host immune surveillance by varying surface antigens, a process termed antigenic variation. While the process of pilin antigenic variation has been extensively studied in the human pathogen Neisseria gonorrhoeae (Gc), relatively few studies of pilin antigenic variation been conducted with Neisseria meningitidis (Mc). Mc is usually a commensal organism that colonizes the human nasopharynx, but when it translocates into the blood stream or meninges, it results in the severe and often deadly meningococcal disease...
September 4, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/30135438/phyllostachys-edulis-forest-reduces-atmospheric-pm-2-5-and-pahs-on-hazy-days-at-suburban-area
#2
Yu Fang Bi, Fei Yan Guo, Liu Yang, Hao Zhong, An Ke Wang, Yu Kui Wang, Zhi Zhuang Wu, Xu Hua Du
This study is aim to illustrate Phyllostachys edulis' role in affecting air quality under hazy day and solar day. P. edulis is a crucial plants growing well at suburban area at China Southern. In this manuscript, on 2 weather conditions (hazy day; solar day), changes in atmospheric particulate matter (PM), polycyclic aromatic hydrocarbons (PAHs), associated volatile organic compounds (VOCs), and PAHs in leaves and soils were measured, with PM-detection equipment and the GC-MC method, in a typical bamboo forest at suburban areas...
August 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29948940/rapid-intracellular-zn-2-dysregulation-via-membrane-corticosteroid-receptor-activation-affects-in-vivo-ca1-ltp
#3
Miki Suzuki, Yuichi Sato, Kotaro Tamura, Haruna Tamano, Atsushi Takeda
Involvement of membrane mineralocorticoid (MC) and glucocorticoid (GC) receptors in synaptic Zn2+ dynamics remains unclear. Here, we tested whether synaptic plasticity is affected by rapid intracellular Zn2+ dysregulation via membrane MC and GC receptor activation, in comparison with intracellular Ca2+ dysregulation. In anesthetized rats, extracellular Zn2+ level was increased under local perfusion of the hippocampal CA1 with 500 ng/ml corticosterone. In vivo CA1 long-term potentiation (LTP) at Schaffer collateral-CA1 pyramidal cell synapses was attenuated by the pre-perfusion with corticosterone prior to tetanic stimulation, and the attenuation was canceled by co-perfusion with CaEDTA, an extracellular Zn2+ chelator, suggesting that corticosterone-induced increase in extracellular Zn2+ is involved in the subsequent attenuation of LTP...
June 8, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29879358/hg-compound-specific-isotope-analysis-at-ultratrace-levels-using-an-on-line-gas-chromatographic-preconcentration-and-separation-strategy-coupled-to-multicollector-inductively-coupled-plasma-mass-spectrometry
#4
Sylvain Bouchet, Sylvain Bérail, David Amouroux
Stable Hg isotope analyses are nowadays widely employed to discriminate Hg sources and understand its biogeochemical cycle. Until now, total Hg isotopic compositions have been mainly used but Hg compound-specific isotopic analysis (CSIA) methodologies are emerging. Online Hg-CSIA were limited to samples containing high concentrations, but in this work we overcome this limitation for the measurement of inorganic (IHg) and monomethylmercury (MMHg) by gas chromatography hyphenated to multicollector-inductively coupled plasma mass spectrometry (GC/MC-ICPMS) through the use of an automated online preconcentration strategy, allowing injection volumes up to 100 times larger than usual...
July 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29849872/methyl-chavicol-and-its-synthetic-analogue-as-possible-antioxidant-and-antilipase-agents-based-on-the-in-vitro-and-in-silico-assays
#5
Bruna Celeida Silva Santos, Andressa Soares Pires, Célia Hitomi Yamamoto, Mara Rubia Costa Couri, Alex Gutterres Taranto, Maria Silvana Alves, Ana Lúcia Dos Santos de Matos Araújo, Orlando Vieira de Sousa
This study investigated the in vitro and in silico biological properties of the methyl chavicol (MC) and its analogue 2-[(4-methoxyphenyl)methyl]oxirane (MPMO), emphasizing the antioxidant and antilipase effects. MPMO was synthesized from MC that reacted with meta -chloroperbenzoic acid and, after separation and purification, was identified by 1 H and 13 C NMR and GC-MS. The antioxidant activity was investigated by DPPH, cooxidation β -carotene/linoleic acid, and thiobarbituric acid assays. With the use of colorimetric determination, the antilipase effect on the pancreatic lipase was tested, while the molecular interaction profiles were evaluated by docking molecular study...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29727931/strengthening-calcium-alginate-microspheres-using-polysulfone-and-its-performance-evaluation-preparation-characterization-and-application-for-enhanced-biodegradation-of-chlorpyrifos
#6
Saira Khalid, Jong-In Han, Imran Hashmi, Ghalib Hasnain, Muhammad Ajaz Ahmed, Sher Jamal Khan, Muhammad Arshad
Bacterial cell immobilization offer considerable advantages over traditional biotreatment systems using free cells. Calcium alginate matrix usually used for bacterial immobilization is susceptible to biodegradation in harsh environment. Current study aimed to produce and characterize stable macrocapsules (MCs) of Chlorpyrifos (CP) degrading bacterial consortium using biocompatible calcium alginate matrix coupled with environmentally stable polysulfone. In current study bacterial consortium capable of CP biodegradation was immobilized using calcium alginate in a form of microcapsule (MC) reinforced by being coated with a synthetic polymer polysulfone (PSf) through phase inversion...
August 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29725133/ultra-capacitive-carbon-neural-probe-allows-simultaneous-long-term-electrical-stimulations-and-high-resolution-neurotransmitter-detection
#7
Surabhi Nimbalkar, Elisa Castagnola, Arvind Balasubramani, Alice Scarpellini, Soshi Samejima, Abed Khorasani, Adrien Boissenin, Sanitta Thongpang, Chet Moritz, Sam Kassegne
We present a new class of carbon-based neural probes that consist of homogeneous glassy carbon (GC) microelectrodes, interconnects and bump pads. These electrodes have purely capacitive behavior with exceptionally high charge storage capacity (CSC) and are capable of sustaining more than 3.5 billion cycles of bi-phasic pulses at charge density of 0.25 mC/cm2 . These probes enable both high SNR (>16) electrical signal recording and remarkably high-resolution real-time neurotransmitter detection, on the same platform...
May 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29580053/occurrence-of-glucocorticoids-mineralocorticoids-and-progestogens-in-various-treated-wastewater-rivers-and-streams
#8
Alexander Weizel, Michael P Schlüsener, Georg Dierkes, Thomas A Ternes
In the current study a high sensitive analytical method was developed for the determination of 60 steroids including glucocorticoids (GC), mineralocorticoids (MC), and progestogens (PG) in WWTP effluents and surface water using liquid chromatography with tandem mass spectrometry detection (LC-MS/MS). The limits of quantification (LOQ) ranged between 0.02 ng/L (cortisone) to 0.5 ng/L (drospirenone) in surface water and from 0.05 ng/L (betamethasone) to 5 ng/L (chlormadinone) in treated wastewater. After optimization, the developed method was applied to WWTP effluents, rivers, and streams around Germany...
May 1, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29551963/cannabinoid-receptors-modulate-excitation-of-an-olfactory-bulb-local-circuit-by-cortical-feedback
#9
Frederic Pouille, Nathan E Schoppa
Recent studies have provided evidence that corticofugal feedback (CFF) from the olfactory cortex to the olfactory bulb (OB) can significantly impact the state of excitation of output mitral cells (MCs) and tufted cells (TCs) and also modulate neural synchrony. Interpreting these effects however has been complicated by the large number of cell targets of CFF axons in the bulb. Within the granule cell layer (GCL) alone, CFF axons target both GABAergic granule cells (GCs) as well as GABAergic deep short-axon cells (dSACs) that inhibit GCs...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29545386/%C3%AE-2-adrenergic-receptor-activation-promotes-long-term-potentiation-at-excitatory-synapses-in-the-mouse-accessory-olfactory-bulb
#10
Guang-Zhe Huang, Mutsuo Taniguchi, Ye-Bo Zhou, Jing-Ji Zhang, Fumino Okutani, Yoshihiro Murata, Masahiro Yamaguchi, Hideto Kaba
The formation of mate recognition memory in mice is associated with neural changes at the reciprocal dendrodendritic synapses between glutamatergic mitral cell (MC) projection neurons and GABAergic granule cell (GC) interneurons in the accessory olfactory bulb (AOB). Although noradrenaline (NA) plays a critical role in the formation of the memory, the mechanism by which it exerts this effect remains unclear. Here we used extracellular field potential and whole-cell patch-clamp recordings to assess the actions of bath-applied NA (10 µM) on the glutamatergic transmission and its plasticity at the MC-to-GC synapse in the AOB...
April 2018: Learning & Memory
https://www.readbyqxmd.com/read/29456621/men-seeking-counselling-in-a-breast-cancer-risk-evaluation-clinic
#11
Ana Catarina Freitas, Ana Opinião, Sofia Fragoso, Hugo Nunes, Madalena Santos, Ana Clara, Sandra Bento, Ana Luis, Jorge Silva, Cecília Moura, Bruno Filipe, Patrícia Machado, Sidónia Santos, Saudade André, Paula Rodrigues, Joana Parreira, Fátima Vaz
Background: Hereditary breast and ovary cancer syndrome affects both genders but little is known about the uptake of genetic services by men. The objective of this study is to characterise the male population counselled through a multidisciplinary breast/ovarian program. Methods: Descriptive analysis of male patients counselled from January 2000 to December 2015. Data in this analysis include new cancer diagnoses during prospective follow up. Results: From 4,320 families registered, 362 male patients were identified: 236 (65...
2018: Ecancermedicalscience
https://www.readbyqxmd.com/read/29409675/features-of-fracture-of-prosthetic-tooth-endocrown-constructions-by-means-of-acoustic-emission-analysis
#12
Valentyn Skalskyi, Valentyn Makeev, Olena Stankevych, Roman Pavlychko
OBJECTIVE: The study aims at comparing the fracture resistance of different restorative materials used in dental endocrown restorations and respective endocrown restorations under a quasi-static compressive load using acoustic emission (AE) method. METHODS: Five restorative materials were used in this study. The restorative materials were manufactured into discs 13mm in diameter and 5mm thick, which were then divided into 5 groups and included into Type 1: Group B: zirconium dioxide (Prettau zirconia); Group C: ceramics (IPS e...
March 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29291262/evidence-for-the-initial-steps-of-dha-biohydrogenation-by-mixed-ruminal-microorganisms-from-sheep-involves-formation-of-conjugated-fatty-acids
#13
Noelia Aldai, Pierluigi Delmonte, Susana P Alves, Rui J B Bessa, John K G Kramer
Incubation of DHA with sheep rumen fluid resulted in 80% disappearance in 6 h. The products were analyzed as their fatty acid (FA) methyl esters by GC-FID on SP-2560 and SLB-IL111 columns. The GC-online reduction × GC and GC-MS techniques demonstrated that all DHA metabolites retained the C22 structure (no evidence of chain-shortening). Two new transient DHA products were identified: mono-trans methylene interrupted-DHA and monoconjugated DHA (MC-DHA) isomers. Identification of MC-DHA was confirmed by their predicted elution using equivalent chain length differences from C18 FA, their molecular ions, and the 22:5 products formed which were the most abundant at 6 h...
January 31, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29190953/distribution-pattern-of-tumor-associated-macrophages-predicts-the-prognosis-of-gastric-cancer
#14
Jiu-Yang Liu, Chun-Wei Peng, Gui-Fang Yang, Wen-Qing Hu, Xiao-Jun Yang, Chao-Qun Huang, Bin Xiong, Yan Li
Purpose: As mayor biomarkers in tumor microenvironment (TME), tumor associated macrophages (TAMs) of gastric cancer (GC) still needs further studies in terms of the number and distribution pattern. Methods: Herein, tissue microarrays (TMA) incorporating 494 GC surgical samples in duplicate were stained for TAMs infiltration analysis. TAMs number was counted according to the locations, including infiltrating macrophages in cancer nest (MC), in invasive front (MF) and in stroma (MS)...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29178727/performance-of-cad-cam-fabricated-fiber-posts-in-oval-shaped-root-canals-an-in-vitro-study
#15
Nino Tsintsadze, Jelena Juloski, Michele Carrabba, Marella Tricarico, Cecilia Goracci, Alessandro Vichi, Marco Ferrari, Simone Grandini
PURPOSE: To assess the push-out strength, the cement layer thickness and the interfacial nanoleakage of prefabricated fiber posts, CAD/CAM fiber posts and metal cast posts cemented into oval-shaped root canals. METHODS: Oval-shaped post spaces were prepared in 30 single-rooted premolars. Roots were randomly assigned to three groups (n=10), according to the post type to be inserted: Group 1: Prefabricated fiber post (D.T. Light-Post X-RO Illusion); Group 2: Cast metal post; Group 3: CAD/CAM-fabricated fiber post (experimental fiber blocks)...
October 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29026857/repellency-effect-of-essential-oils-of-some-native-plants-and-synthetic-repellents-against-human-flea-pulex-irritans-siphonaptera-pulicidae
#16
Mohammad Bagher Ghavami, Fahimeh Poorrastgoo, Behrooz Taghiloo, Jamshid Mohammadi
BACKGROUND: Fleas are important vectors of human and animal disease, and control measures for protection against their bites and flea-borne diseases are necessary. METHODS: The essential oils (EOs) of four native medicinal plants, Ziziphora tenuiore , Myrtus communis , Achillea wilhelmsii and Mentha piperita were isolated by hydrodistillation technique and analyzed by GC-MC. The repellent activity of EOs and synthetic compounds, DEET and permethrin, were assayed on human subjects against field collected fleas...
March 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/28917754/quantification-of-7-aminoflunitrazepam-in-human-urine-by-polymeric-monolith-based-capillary-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#17
Yu-Ru Wu, Hsiang-Yu Liu, Shu-Ling Lin, Ming-Ren Fuh
Using a simple liquid-liquid extraction (LLE) procedure for sample pretreatment, 7-Aminoflunitrazepam (7-aminoFM2), a major metabolite of flunitrazepam (FM2), was determined in urine samples by polymeric monolith-based capillary liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The linearity was found in the range of 0.1-50ngmL-1 with a method detection limit (signal-to-noise ratio of 3) estimated at 0.05ngmL-1 . Using the proposed method, good precision and recovery were also found in spiked urine samples at the levels of 0...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28837312/compound-specific-stable-chlorine-isotopic-analysis-of-volatile-aliphatic-compounds-using-gas-chromatography-hyphenated-with-multiple-collector-inductively-coupled-plasma-mass-spectrometry
#18
Axel Horst, Julian Renpenning, Hans-Hermann Richnow, Matthias Gehre
Stable chlorine isotope analysis is increasingly used to characterize sources, transformation pathways, and sinks of organic aliphatic compounds, many of them being priority pollutants in groundwater and the atmosphere. A wider use of chlorine isotopes in environmental studies is still inhibited by limitations of the different analytical techniques such as high sample needs, offline preparation, confinement to few compounds and mediocre precision, respectively. Here we present a method for the δ(37)Cl determination in volatile aliphatic compounds using gas chromatography coupled with multiple-collector inductively coupled plasma mass spectrometry (GC-MC-ICPMS), which overcomes these limitations...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28817805/ltp-at-hilar-mossy-cell-dentate-granule-cell-synapses-modulates-dentate-gyrus-output-by-increasing-excitation-inhibition-balance
#19
Yuki Hashimotodani, Kaoutsar Nasrallah, Kyle R Jensen, Andrés E Chávez, Daniel Carrera, Pablo E Castillo
Excitatory hilar mossy cells (MCs) in the dentate gyrus receive inputs from dentate granule cells (GCs) and project back to GCs locally, contralaterally, and along the longitudinal axis of the hippocampus, thereby establishing an associative positive-feedback loop and connecting functionally diverse hippocampal areas. MCs also synapse with GABAergic interneurons that mediate feed-forward inhibition onto GCs. Surprisingly, although these circuits have been implicated in both memory formation (e.g., pattern separation) and temporal lobe epilepsy, little is known about activity-dependent plasticity of their synaptic connections...
August 16, 2017: Neuron
https://www.readbyqxmd.com/read/28800525/chemical-analysis-and-potential-endocrine-activities-of-aluminium-coatings-intended-to-be-in-contact-with-cosmetic-water
#20
Elias Bou-Maroun, Laurence Dahbi, Marie-Pierre Gomez-Berrada, Philippine Pierre, Sandrine Rakotomalala, Pierre-Jacques Ferret, Marie-Christine Chagnon
The objective of the work was to check the presence of Non-Intended Added Substances (NIAS) with hormonal activities in aluminium coatings extracts coded: AA, BBF, MC and RR, furnished by four different suppliers. Water samples were prepared at room temperature or at 40°C for three months to verify the storage effect on the coatings. Solid phase extraction was used to concentrate and to extract coating substances. Hormonal activities were checked in vitro using reporter gene bioassays. Except BBF, all extracts induced a weak but significant estrogenic agonist activity in the human cell line...
October 25, 2017: Journal of Pharmaceutical and Biomedical Analysis
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