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Haemaphysalis flava

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https://www.readbyqxmd.com/read/29422072/proteomics-analysis-of-faecal-proteins-in-the-tick-haemaphysalis-flava
#1
Lei Liu, Yi-Song Liu, Guo-Hua Liu, Tian-Yin Cheng
BACKGROUND: Ticks and tick-borne diseases are of major public health concern. Currently, development of vaccines against ticks is considered crucial for their control. A critical step in this process is the screening of viable antigens. Faeces are byproducts of digestion and blood meal utilization, and partly reflect the vitality and vector potential of ticks. However, an integrated analysis of proteins in tick faeces is lacking. The present study explored the protein components in the faeces of the tick Haemaphysalis flava, by liquid chromatography-tandem mass spectrometry (LC/MS-MS) to identify potential protein antigens for vaccine development against ticks...
February 8, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29371124/proteomic-profiling-of-the-midgut-contents-of-haemaphysalis-flava
#2
Lei Liu, Tian-Yin Cheng, Xiao-Ming He
Scant information is available regarding the proteins involved in blood meal processing in ticks. Here, we aimed to highlight the midgut proteins involved in preventing blood meal coagulation, and in facilitating intracellular digestion in the tick Haemaphysalis flava. Proteins were extracted from the midgut contents of fully engorged and partially engorged ticks. We used liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify 131 unique peptides, and 102 proteins. Of these, 15 proteins, each with at least two unique peptides, were recognized with high confidence...
March 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29197549/isolation-and-characterization-of-kabuto-mountain-virus-a-new-tick-borne-phlebovirus-from-haemaphysalis-flava-ticks-in-japan
#3
Hiroko Ejiri, Chang-Kweng Lim, Haruhiko Isawa, Yukie Yamaguchi, Ryosuke Fujita, Mutsuyo Takayama-Ito, Ryusei Kuwata, Daisuke Kobayashi, Madoka Horiya, Guillermo Posadas-Herrera, Itoe Iizuka-Shiota, Satsuki Kakiuchi, Yukie Katayama, Toshihiko Hayashi, Toshinori Sasaki, Mutsuo Kobayashi, Shigeru Morikawa, Ken Maeda, Tetsuya Mizutani, Koki Kaku, Masayuki Saijo, Kyoko Sawabe
In Japan, indigenous tick-borne phleboviruses (TBPVs) and their associated diseases first became evident in 2013 by reported human cases of severe fever with thrombocytopenia syndrome (SFTS). In this study, we report a novel member of the genus Phlebovirus designated as Kabuto Mountain virus (KAMV), which was isolated from the ixodid tick Haemaphysalis flava in Hyogo, Japan. A complete viral genome sequencing and phylogenetic analyses showed that KAMV is a novel member of TBPVs, which is closely related to the Uukuniemi and Kaisodi group viruses...
January 15, 2018: Virus Research
https://www.readbyqxmd.com/read/28981682/francisella-like-endosymbiont-detected-in-haemaphysalis-ticks-acari-ixodidae-from-the-republic-of-korea
#4
Ratree Takhampunya, Heung-Chul Kim, Sung-Tae Chong, Achareeya Korkusol, Bousaraporn Tippayachai, Silas A Davidson, Jeannine M Petersen, Terry A Klein
A total of 6,255 ticks belonging to three genera and six species (Haemaphysalis flava Neumann, Haemaphysalis longicornis Neumann, Haemaphysalis phasiana Saito, Ixodes nipponensis Kitaoka & Saito, Ixodes persulcatus Schulze, and Amblyomma testudinarium Koch) collected from May-August, 2013, at four southwestern provinces in the Republic of Korea (ROK) were submitted to the Armed Forces Research Institute of Medical Sciences and assayed for selected tick-borne pathogens. One pool each of H. flava and H. phasiana was positive by PCR and sequencing for a Francisella-like endosymbiont, while all pools were negative for Francisella tularensis, the causative agent of tularemia...
November 7, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28964878/isolation-and-characterization-of-tarumizu-tick-virus-a-new-coltivirus-from-haemaphysalis-flava-ticks-in-japan
#5
Ryosuke Fujita, Hiroko Ejiri, Chang-Kweng Lim, Shinichi Noda, Takeo Yamauchi, Mamoru Watanabe, Daisuke Kobayashi, Mutsuyo Takayama-Ito, Katsunori Murota, Guillermo Posadas-Herrera, Shohei Minami, Ryusei Kuwata, Yukie Yamaguchi, Madoka Horiya, Yukie Katayama, Hiroshi Shimoda, Masayuki Saijo, Ken Maeda, Tetsuya Mizutani, Haruhiko Isawa, Kyoko Sawabe
During the course of tick-borne virus surveillance in Japan, three independent isolates of probably the same virus were obtained from three geographically distant populations of the hard tick Haemaphysalis flava. Genome analyses of the three isolates demonstrated that they were closely related but distinct strains of a novel virus, designated Tarumizu tick virus (TarTV), which has a genome of 12 double-stranded RNA segments. The development of the virus-induced cytopathic effects on BHK cells significantly varied according to virus strains...
October 15, 2017: Virus Research
https://www.readbyqxmd.com/read/28506041/identification-of-tick-species-collected-from-wild-boars-and-habitats-of-wild-boars-and-domestic-pigs-in-the-republic-of-korea
#6
Jeong-Byoung Chae, Jun-Gu Kang, Heung-Chul Kim, Sung-Tae Chong, In-Yong Lee, Nam-Shik Shin, Joon-Seok Chae
Tick is one of the most important arthropods in the transmission of vector-borne diseases. In this study, we investigated the abundance and species of ticks associated with swine and their habitats to assess the risk of spread of tick-borne diseases in host species, such as wild boars. Ticks were collected from 24 grazing or traditionally reared domestic pig farms and 8 habitats of wild boars in 8 provinces and 1 city in the Republic of Korea, by using the dragging and flagging methods. Ticks were also collected directly from 49 wild boars by using fine forceps...
April 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28462495/cloning-and-expression-pattern-of-a-heat-shock-cognate-protein-70-gene-in-ticks-haemaphysalis-flava
#7
Lei Liu, Tian-Yin Cheng, Ya Yang
Ticks and tick-borne-diseases have serious public health implications, and screening feasible protein candidates for vaccines development is identified to be an effective alternative to control of tick infestations. In current study, we focused on cloning the full-length gene encoding a heat shock cognate protein 70 (Hsc70), a molecular chaperone of critical functional roles belonging to heat shock protein 70 (HSP70) family, in salivary glands of Haemaphysalis flava, namely Hf-Hsc70, and analyzing the expression of Hf-Hsc70 in different life phases, organs and ambient temperatures...
June 2017: Parasitology Research
https://www.readbyqxmd.com/read/28426403/pcr-denaturing-gradient-gel-electrophoresis-as-a-useful-method-to-identify-of-intestinal-bacteria-flora-in-haemaphysalis-flava-ticks
#8
Tian-Yin Cheng, Guo-Hua Liu
Ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. In the present study, the intestinal bacterial flora associated with Haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). Eleven distinct DGGE bands were found using PCR-DGGE method. Sequences analyses indicated that they belonged to Bacillus cereus, Candidatus rickettsia, Erwinia sp...
June 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28411028/extensive-diversity-of-rickettsiales-bacteria-in-ticks-from-wuhan-china
#9
Miao Lu, Jun-Hua Tian, Bin Yu, Wen-Ping Guo, Edward C Holmes, Yong-Zhen Zhang
Rickettsiales bacteria are important agents of (re)emerging infectious diseases, with ticks playing a key role in their evolution and transmission. We collected 1079 hard ticks belonging to five species (Ixodes sinensis, Rhipicephalus microplus, Haemaphysalis flava, Haemaphysalis hystricis and Haemaphysalis longicornis) from cattle and goats in Wuhan city, Hubei province, China. The dominant tick species was H. longicornis (578, 53.57%), followed by R. microplus (354, 32.81%), H. hystricis (62, 5.75%), H. flava (57, 5...
June 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28362864/epidemiological-study-of-relapsing-fever-borreliae-detected-in-haemaphysalis-ticks-and-wild-animals-in-the-western-part-of-japan
#10
Kiwa Furuno, Kyunglee Lee, Yukie Itoh, Kazuo Suzuki, Kenzo Yonemitsu, Ryusei Kuwata, Hiroshi Shimoda, Masahisa Watarai, Ken Maeda, Ai Takano
The genus Borrelia comprises arthropod-borne bacteria, which are infectious agents in vertebrates. They are mainly transmitted by ixodid or argasid ticks. In Hokkaido, Japan, Borrelia spp. were found in deer and Haemaphysalis ticks between 2011 and 2013; however, the study was limited to a particular area. Therefore, in the present study, we conducted large-scale surveillance of ticks and wild animals in the western part of the main island of Japan. We collected 6,407 host-seeking ticks from two regions and 1,598 larvae obtained from 32 engorged female ticks and examined them to elucidate transovarial transmission...
2017: PloS One
https://www.readbyqxmd.com/read/28091959/genetic-variation-in-mitochondrial-genes-of-the-tick-haemaphysalis-flava-collected-from-wild-hedgehogs-in-china
#11
Zhong-Bo Li, Tian-Yin Cheng, Xing-Li Xu, Lu-Lin Song, Guo-Hua Liu
The tick Haemaphysalis flava (Acari: Ixodidae) is an important ectoparasite, which causes direct damage to their hosts and also acts as a vector of various infectious disease agents in China. Despite its significance, the epidemiology, genetics and biology of H. flava has not been studied in detail. In the present study, the genetic variation in three mitochondrial (mt) DNA regions, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 and 4 (nad1 and nad4), was examined in H. flava ticks collected from wild hedgehogs in China...
February 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28069059/molecular-detection-of-rickettsia-species-in-ticks-collected-from-the-southwestern-provinces-of-the-republic-of-korea
#12
Yoontae Noh, Yeong Seon Lee, Heung-Chul Kim, Sung-Tae Chong, Terry A Klein, Ju Jiang, Allen L Richards, Hae Kyeong Lee, Su Yeon Kim
BACKGROUND: Rickettsiae constitute a group of arthropod-borne, Gram-negative, obligate intracellular bacteria that are the causative agents of diseases ranging from mild to life threatening that impact on medical and veterinary health worldwide. METHODS: A total of 6,484 ticks were collected by tick drag from June-October 2013 in the southwestern provinces of the Republic of Korea (ROK) (Jeollanam, n = 3,995; Jeollabuk, n = 680; Chungcheongnam, n = 1,478; and Chungcheongbuk, n = 331)...
January 10, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/27966221/determination-of-the-microbial-community-features-of-haemaphysalis-flava-in-different-developmental-stages-by-high-throughput-sequencing
#13
Deyong Duan, Tianyin Cheng
In this study, we analyzed the in vivo microbial community structure and diversity differences of Haemaphysalis flava (H. flava) in four developmental stages (egg, larva, nymph, and adult) to determine which bacterial genera could be propagated through transovarial transmission and transmitted by transstadial transmission during different developmental stages of H. flava. Paired-end sequencing of the V3 region of the 16S ribosomal DNA (16S rDNA) of H. flava in four developmental stage samples was conducted using the Illumina MiSeq sequencing platform...
April 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27631766/expression-pattern-of-suba-in-different-tissues-and-blood-feeding-status-in-haemaphysalis-flava
#14
Lei Liu, Tian-Yin Cheng, Fen Yan
Tick-borne-diseases (TBD) pose a huge threat to the health of both humans and animals worldwide. Tick vaccines constitute an attractive alternative for tick control, due to their cost-efficiency and environmental-friendliness. Subolesin, a protective antigen against ticks, is reported to be a promising candidate for the development of broad-spectrum vaccines. However, the entire length of its gene, subA, and its gene expression pattern in different tissues and blood-feeding status (or different levels of engorgement) have not been studied extensively...
December 2016: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/27432517/detection-of-francisella-tularensis-and-analysis-of-bacterial-growth-in-ticks-in-japan
#15
J Suzuki, M Hashino, S Matsumoto, A Takano, H Kawabata, N Takada, M Andoh, Y Oikawa, H Kajita, A Uda, K Watanabe, T Shimizu, M Watarai
UNLABELLED: Francisella tularensis is distributed in the Northern hemisphere and it is the bacterial agent responsible for tularaemia, a zoonotic disease. We collected 4 527 samples of DNA from ticks in Japan, which were then analysed by real-time PCR and nested PCR. Francisella DNA was detected by real-time PCR in 2·15% (45/2 093) of Ixodes ovatus, 0·66% (14/2 107) of I. persulcatus, 8·22% (6/73) of I. monospinosus and 0·72% (1/138) of Haemaphysalis flava specimens. Finally, Francisella DNA was detected by nested PCR in 42 and five samples I...
October 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/27211914/molecular-detection-of-severe-fever-with-thrombocytopenia-syndrome-and-tick-borne-encephalitis-viruses-in-ixodid-ticks-collected-from-vegetation-republic-of-korea-2014
#16
Seok-Min Yun, Ye-Ji Lee, WooYoung Choi, Heung-Chul Kim, Sung-Tae Chong, Kyu-Sik Chang, Jordan M Coburn, Terry A Klein, Won-Ja Lee
Ticks play an important role in transmission of arboviruses responsible for emerging infectious diseases, and have a significant impact on human, veterinary, and wildlife health. In the Republic of Korea (ROK), little is known about information regarding the presence of tick-borne viruses and their vectors. A total of 21,158 ticks belonging to 3 genera and 6 species collected at 6 provinces and 4 metropolitan areas in the ROK from March to October 2014 were assayed for selected tick-borne pathogens. Haemaphysalis longicornis (n=17,570) was the most numerously collected, followed by Haemaphysalis flava (n=3317), Ixodes nipponensis (n=249), Amblyomma testudinarium (n=11), Haemaphysalis phasiana (n=8), and Ixodes turdus (n=3)...
July 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27043361/detection-of-severe-fever-with-thrombocytopenia-syndrome-virus-from-wild-animals-and-ixodidae-ticks-in-the-republic-of-korea
#17
Sung-Suck Oh, Jeong-Byoung Chae, Jun-Gu Kang, Heung-Chul Kim, Sung-Tae Chong, Jeong-Hwa Shin, Moon-Suk Hur, Jae-Hwa Suh, Myoung-Don Oh, Soo-Myoung Jeong, Nam-Shik Shin, Kyoung-Seong Choi, Joon-Seok Chae
Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV), a novel bunyavirus reported to be endemic to central-northeastern China, southern Japan, and the Republic of Korea (ROK). To investigate SFTSV infections, we collected serum samples and ticks from wild animals. Using serum samples and ticks, SFTSV-specific genes were amplified by one-step RT-PCR and nested PCR and sequenced. Indirect immunofluorescence assay (IFA) was performed to analyze virus-specific antibody levels in wild animals...
June 2016: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/27035712/ecology-of-the-tick-borne-phlebovirus-causing-severe-fever-with-thrombocytopenia-syndrome-in-an-endemic-area-of-china
#18
Zhifeng Li, Changjun Bao, Jianli Hu, Wendong Liu, Xiaochen Wang, Lei Zhang, Zhengmin Ji, Zhi Feng, Luxun Li, Aihua Shen, Xuejian Liu, Hongjun Zhao, Wenwen Tan, Jiangang Zhou, Xian Qi, Yefei Zhu, Fenyang Tang, Carol J Cardona, Zheng Xing
BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV), a tick-borne phlebovirus in family Bunyaviridae. Studies have found that humans, domestic and wildlife animals can be infected by SFTSV. However, the viral ecology, circulation, and transmission remain largely unknown. METHODOLOGY/PRINCIPAL FINDINGS: Sixty seven human SFTS cases were reported and confirmed by virus isolation or immunofluorescence assay between 2011 and 2014...
April 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/26951985/prevalence-of-anaplasma-and-bartonella-spp-in-ticks-collected-from-korean-water-deer-hydropotes-inermis-argyropus
#19
Jun-Gu Kang, Sungjin Ko, Heung-Chul Kim, Sung-Tae Chong, Terry A Klein, Jeong-Byoung Chae, Yong-Sun Jo, Kyoung-Seong Choi, Do-Hyeon Yu, Bae-Keun Park, Jinho Park, Joon-Seok Chae
Deer serve as reservoirs of tick-borne pathogens that impact on medical and veterinary health worldwide. In the Republic of Korea, the population of Korean water deer (KWD, Hydropotes inermis argyropus) has greatly increased from 1982 to 2011, in part, as a result of reforestation programs established following the Korean War when much of the land was barren of trees. Eighty seven Haemaphysalis flava, 228 Haemaphysalis longicornis, 8 Ixodes nipponensis, and 40 Ixodes persulcatus (21 larvae, 114 nymphs, and 228 adults) were collected from 27 out of 70 KWD...
February 2016: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/26822735/enolase-a-plasminogen-receptor-isolated-from-salivary-gland-transcriptome-of-the-ixodid-tick-haemaphysalis-flava
#20
Xing-Li Xu, Tian-Yin Cheng, Hu Yang
Enolase, a multifunctional protein, is shown to act as a plasminogen receptor that contributes to fibrinolysis, which plays an important role in preventing the formation of blood clots during tick feeding. The study of enolase genes provides opportunities to develop a potential antigen target for tick control. So far, enolase has been identified in only a few species of ticks. Knowledge of the exact mechanisms of plasminogen activation and fibrinolysis by enolase as a plasminogen receptor is limited. Here, we cloned the enolase full-length complementary DNA (cDNA) from the salivary glands of Haemaphysalis flava, expressed it, and analyzed the function of the recombinant H...
May 2016: Parasitology Research
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