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https://www.readbyqxmd.com/read/30312344/severity-of-clinical-dry-eye-manifestations-influences-protein-expression-in-tear-fluid-of-patients-with-primary-sj%C3%A3-gren-s-syndrome
#1
Lara A Aqrawi, Xiangjun Chen, Janicke Liaaen Jensen, Mathias Kaurstad Morthen, Bernd Thiede, Øygunn Aass Utheim, Øyvind Palm, Behzod Tashbayev, Tor Paaske Utheim, Hilde Kanli Galtung
Ocular dryness is a characteristic feature of primary Sjögren's syndrome (pSS). This may result in dry eye disease (DED), leading to damage of the ocular surface. Additional, non-invasive diagnostic techniques are needed when evaluating pSS patients. Hence, screening for disease-specific biomarkers in biological fluid could be promising. We have previously examined the proteome of tear fluid from pSS patients through Liquid chromatography-mass spectrometry (LC-MS), and conducted a thorough ocular evaluation of patients with pSS...
2018: PloS One
https://www.readbyqxmd.com/read/30311616/structure-and-stimuli-responsiveness-of-all-dna-dendrimers-theory-and-experiment
#2
Clemens Jochum, Nataša AdŽić, Emmanuel Stiakakis, Thomas L Derrien, Dan Luo, Gerhard Kahl, Christos N Likos
We present a comprehensive theoretical and experimental study of the solution phase properties of a DNA-based family of nanoparticles - dendrimer-like DNA molecules (DL-DNA). These charged DNA dendrimers are novel macromolecular aggregates, which hold high promise in targeted self-assembly of soft matter systems in the bulk and at interfaces. To describe the behaviour of this family of dendrimers (with generations ranging from G1 to G7), we use a theoretical model in which base-pairs of a single DL-DNA molecule are modeled by charged monomers, whose interactions are chosen to mimic the equilibrium properties of DNA correctly...
October 12, 2018: Nanoscale
https://www.readbyqxmd.com/read/30309858/deep-learning-to-distinguish-recalled-but-benign-mammography-images-in-breast-cancer-screening
#3
Sarah S Aboutalib, Aly A Mohamed, Wendie A Berg, Margarita L Zuley, Jules H Sumkin, Shandong Wu
Purpose: False positives in digital mammography screening lead to high recall rates, resulting in unnecessary medical procedures to patients and health care costs. This study aimed to investigate the revolutionary deep learning methods to distinguish recalled but benign mammography images from negative exams and those with malignancy. Experimental Design: Deep learning convolutional neural network (CNN) models were constructed to classify mammography images into malignant (breast cancer), negative (breast cancer free), and recalled-benign categories...
October 11, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/30298715/characterization-of-mechanical-degradation-in-perfluoropolyether-film-for-its-application-to-anti-fingerprint-coatings
#4
Kyoungmin Min, Jungim Han, Byungha Park, Eunseog Cho
Enhancing the mechanical durability of anti-fingerprint films is critical for its industrial application on touch-screen devices to withstand friction damage from repeated rubbing in daily usage. Using reactive molecular dynamics simulations, we herein implement adhesion, mechanical, and deposition tests to investigate two durability-determining factors: intrachain and interchain strength, which affect the structural stability of the anti-fingerprint film (perfluoropolyether) on silica. From the intrachain perspective, it is found that the Si-C bond in the polymer chain is the weakest, and therefore prone to dissociation and potentially forming a C-O bond...
October 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30297812/polarity-governs-atomic-interaction-through-two-dimensional-materials
#5
Wei Kong, Huashan Li, Kuan Qiao, Yunjo Kim, Kyusang Lee, Yifan Nie, Doyoon Lee, Tom Osadchy, Richard J Molnar, D Kurt Gaskill, Rachael L Myers-Ward, Kevin M Daniels, Yuewei Zhang, Suresh Sundram, Yang Yu, Sang-Hoon Bae, Siddharth Rajan, Yang Shao-Horn, Kyeongjae Cho, Abdallah Ougazzaden, Jeffrey C Grossman, Jeehwan Kim
The transparency of two-dimensional (2D) materials to intermolecular interactions of crystalline materials has been an unresolved topic. Here we report that remote atomic interaction through 2D materials is governed by the binding nature, that is, the polarity of atomic bonds, both in the underlying substrates and in 2D material interlayers. Although the potential field from covalent-bonded materials is screened by a monolayer of graphene, that from ionic-bonded materials is strong enough to penetrate through a few layers of graphene...
October 8, 2018: Nature Materials
https://www.readbyqxmd.com/read/30296059/microfluidic-based-genosensor-to-detect-human-papillomavirus-hpv16-for-head-and-neck-cancer
#6
Andrey Coatrini Soares, Juliana Coatrini Soares, Valquiria Cruz Rodrigues, Heveline Dal Magro Follmann, Lidia Maria Rebolho Batista Arantes, Ana Carolina Carvalho, Matias Eliseo Melendez, José Humberto Tavares Guerreiro Fregnani, Rui M Reis, Andre L Carvalho, Osvaldo Novais Oliveira
High-risk HPV infection, mainly with HPV16 type, has been increasingly considered as an important etiologic factor in head and neck cancers. Detection of HPV16 is therefore crucial for these types of cancer, but clinical tests are not performed routinely in public health systems owing to the high cost and limitations of existing tests. In this paper, we report on a potentially low cost genosensor capable of detecting low concentrations of HPV16 in buffer samples and distinguishing, with high accuracy, head and neck cancer cell lines according to their HPV16 status...
October 8, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30295655/highly-sensitive-and-quantitative-detection-of-proteins-and-their-isoforms-by-capillary-isoelectric-focusing-method
#7
Narendra Padhan
Immunoblotting has become a routine technique in many laboratories for protein characterization from biological samples. The following protocol provides an alternative strategy, capillary isoelectric focusing (cIEF), with many advantages compared to conventional immunoblotting. This is an antibody-based, automated, rapid, and quantitative method in which a complete western blotting procedure takes place inside an ultrathin capillary. This technique does not require a gel to transfer to a membrane, stripping of blots, or x-ray films, which are typically required for conventional immunoblotting...
September 19, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/30293475/a-standardised-database-of-chinese-emotional-film-clips
#8
Yan Ge, Guozhen Zhao, Yulin Zhang, Rebecca J Houston, Jinjing Song
Film clips are widely used in emotion research due to their relatively high ecological validity. Although researchers have established various film clip sets for different cultures, the few that exist related to Chinese culture do not adequately address positive emotions. The main purposes of the present study were to establish a standardised database of Chinese emotional film clips that could elicit more categories of reported positive emotions compared to the existing databases and to expand the available film clips that can be used as neutral materials...
October 8, 2018: Cognition & Emotion
https://www.readbyqxmd.com/read/30291659/surface-enhanced-raman-spectroscopy-for-the-detection-of-a-metabolic-product-in-the-headspace-above-live-bacterial-cultures
#9
Jessica Kelly, Robin Patrick, Sheila Patrick, Steven E J Bell
In-situ surface-enhanced Raman spectroscopy of the headspace above cultures of 6 bacterial species allowed sensitive and selective detection of characteristic bands from chemisorbed methyl sulphide. This marker compound is created by dissociation of adsorbed dimethyl disulphide (DMDS) on the surface of the Au or Ag nanoparticle (NP) films which are used as the enhancing media. DMDS is a well-known fermentative metabolite of bacteria and was also detected here by GC-MS of the headspace of bacteria cultures. Kinetic binding plots of media spiked with DMDS and of live cultures, both showed that the more rapid adsorption of DMDS onto Au based substrates made them more suitable for the rapid detection of bacteria than Ag based substrates...
October 5, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/30272644/protocol-for-producing-three-dimensional-infrared-video-of-freezing-in-plants
#10
David P Livingston, Tan D Tuong, Mark Hoffman, Gina Fernandez
Freezing in plants can be monitored using infrared (IR) thermography, because when water freezes, it gives off heat. However, problems with color contrast make 2-dimensions (2D) infrared images somewhat difficult to interpret. Viewing an IR image or the video of plants freezing in 3 dimensions (3D) would allow a more accurate identification of sites for ice nucleation as well as the progression of freezing. In this paper, we demonstrate a relatively simple means to produce a 3D infrared video of a strawberry plant freezing...
September 12, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/30269960/reduced-graphene-oxide-transducers-for-biosensing-applications-beyond-the-debye-screening-limit
#11
Xiaoling Lu, Anna Miodek, Walid-Madhat Munief, Pawan Jolly, Vivek Pachauri, Xianping Chen, Pedro Estrela, Sven Ingebrandt
In the field of label-free biosensing, various transducer materials and strategies are under investigation to overcome the Debye-screening limitation of charged biomolecules. We demonstrate an in-line, impedimetric aptasensor with reduced graphene-oxide (rGO) thin films as transducers to detect prostate specific antigens (PSA) in a physiological buffer solution. Unlike classical electrochemical impedance spectroscopy (EIS), this direct, label-free and fully-electronic biosensor approach does not need any redox markers...
September 14, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30265778/toward-endothelialization-via-vascular-endothelial-growth-factor-immobilization-on-cell-repelling-functional-polyurethanes
#12
Shurui Liao, Qiang He, Lie Yang, Shuai Liu, Ze Zhang, Robert Guidoin, Qiang Fu, Xingyi Xie
We screened a family of nonspecific cell-repelling polyurethanes (PUs) whose backbones are attached with epoxy group-terminated polyethylene glycol (PEG) side chains. Water incubation of the PU films (with 9.2-31.1 wt % PEG) caused a surface enrichment of PEG chains where vascular endothelial growth factor (VEGF) was grafted by forming secondary amine linkages between VEGF molecules and the PEG spacer. These linkages are still ionizable similar to original primary amines in VEGF, thereby retaining the original charge distribution on VEGF macromolecules...
September 28, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/30262870/minority-and-majority-charge-carrier-mobility-in-cu-2-znsnse-4-revealed-by-terahertz-spectroscopy
#13
Hannes Hempel, Charles J Hages, Rainer Eichberger, Ingrid Repins, Thomas Unold
The mobilities of electrons and holes determine the applicability of any semiconductor, but their individual measurement remains a major challenge. Here, we show that time-resolved terahertz spectroscopy (TRTS) can distinguish the mobilities of minority and majority charge carriers independently of the doping-type and without electrical contacts. To this end, we combine the well-established determination of the sum of electron and hole mobilities from photo-induced THz absorption spectra with mobility-dependent ambipolar modeling of TRTS transients...
September 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30253913/magnesium-aluminum-layered-double-hydroxide-graphene-oxide-composite-mixed-matrix-membrane-for-the-thin-film-microextraction-of-diclofenac-in-biological-fluids
#14
Milad Ghani, Sayed Mehdi Ghoreishi, Mostafa Azamati
Herein, the applicability of Mg-Al-layered double hydroxide-graphene oxide (LDH/GO) mixed-matrix membrane (MMM) for microextraction purposes is reported for the first time. The LDH/GO MMM was used as sorbent for the thin film microextraction (TFME) of diclofenac in human body fluids. The prepared LDH/GO composite has been incorporated into a mechanically stable polyvinylidene difluoride (PVDF) membrane. The contribution of GO in LDH/GO composites significantly improved the extraction efficiency of the TFME sorbent...
September 17, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/30253827/integrated-three-electrode-screen-printed-sensor-modified-with-bismuth-film-for-voltammetric-determination-of-thallium-i-at-the-ultratrace-level
#15
K Domańska, K Tyszczuk-Rotko
In the present paper, for the first time, the integrated three-electrode screen-printed sensor with in situ plated bismuth film carbon working electrode was applied for the ultratrace determination of thallium(I) (Tl(I)). Under optimized conditions extremely low limits of detection were reached, 8.47 × 10-10 and 6.71 × 10-12  mol L-1 for the deposition times of 60 s and 300 s, respectively. The influences of foreign metal ions and surfactants on the voltammetric signal of thallium in natural samples were minimized using 1 × 10-5  mol L-1 EDTA and Amberlite XAD-7 resin added to the buffer solution (CH3 COONH4 , CH3 COOH and NH4 Cl) of pH = 4...
December 7, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/30247869/charge-carrier-lifetimes-in-cr-fe-al-o-thin-films
#16
Helge Stein, Sönke Müller, Klaus Schwarzburg, Dennis Friedrich, Alfred Ludwig, Rainer Eichberger
The effect of compositional variation on charge carrier lifetimes of Cr1 Fe0.84 Al0.16 O3 , a promising material for solar water splitting recently identified using combinatorial materials science, is explored using ultrafast time-resolved optical reflectance. The transient signal can be described by a biexponential decay where the shorter time constant varies over one order of magnitude with changing Cr content while the longer one stays constant. Intrinsic performance limitations such as a low charge carrier mobility on the order of 10-3 cm2 /(Vs) are identified...
September 24, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30226386/salt-induced-continuous-deposition-of-supramolecular-iron-iii-tannic-acid-complex
#17
Taegyun Park, Won Il Kim, Beom Jin Kim, Hojae Lee, Insung S Choi, Ji Hun Park, Woo Kyung Cho
One-step assembly of iron(III)-tannic acid (Fe3+ -TA) complex forms nanothin (∼10 nm) films on various substrates within minutes. In this deposition scheme, however, the film does not grow continuously over time even though Fe3+ -TA complex is still abundant in the coating solution. In this paper, we report that the salt addition dramatically changes the one-off coating characteristic to continuous one, and each salt has its optimum concentration ( CMFT ) that produces maximum film thickness. For detailed investigation of the salt effects, we employed various salts, including LiCl, NaCl, KCl, CaCl2 , SrCl2 , BaCl2 , NaBr, and NaNO3 , and found that only cations played an important role in the continuous deposition of the Fe3+ -TA complex, with smaller CMFT values for the cations of higher valency and larger size...
October 2, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/30223340/photodegradation-of-brominated-flame-retardants-in-polystyrene-quantum-yields-products-and-influencing-factors
#18
Amina Khaled, Claire Richard, Agnès Rivaton, Farouk Jaber, Mohamad Sleiman
Brominated flame retardants (BFRs) are widely used as additives in plastics, textiles and electronics materials. Here, we investigated the photodegradation of four BFRs including decabromobiphenylether (BDE-209), tetrabromobipsphenol A (TBBPA), tetrabromobisphenol A-bis(2,3-dibromopropylether) (TBBPA-DBPE) and tetrabromobisphenol A bis (allyl) ether (TBBPA-BAE). Experiments were carried out in polystyrene (PS) films using monochromatic and polychromatic irradiations. For comparison, irradiations were also carried in a solvent (tetrahydrofuran: THF)...
November 2018: Chemosphere
https://www.readbyqxmd.com/read/30214199/layer-by-layer-dna-films-incorporating-highly-transfecting-bioreducible-poly-amido-amine-and-polyethylenimine-for-sequential-gene-delivery
#19
Lingxiao Xie, Xiong Ding, Rachel Budry, Guangzhao Mao
Background: The layer-by-layer (LbL) assembly method offers a molecular level control of the amount and spatial distribution of bioactive molecules. However, successful clinical translation of LbL film technology will most certainly require a better understanding and control of not only the film assembly process, but also film disassembly kinetics in physiologic conditions. Purpose: This work focuses on the understanding and control of degradation properties of LbL films for localized gene delivery...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/30194447/maximum-blink-interval-is-associated-with-tear-film-breakup-time-a-new-simple-screening-test-for-dry-eye-disease
#20
Takenori Inomata, Masao Iwagami, Yoshimune Hiratsuka, Keiichi Fujimoto, Yuichi Okumura, Tina Shiang, Akira Murakami
The prevalence of dry eye disease (DED) is increasing worldwide, and its diagnosis often needs dedicated reagents and machines. We investigated the usefulness of maximum blink interval (MBI) (the length of time that participants could keep their eyes open) in screening for DED. This cross-sectional study included 292 patients (194 with DED and 98 without DED) recruited between September 2016 and September 2017. We compared the MBI between patients with and without DED; examined correlations between MBI and other clinical features of DED, including subjective symptoms (Dry Eye-Related Quality-of-Life Score), tear film breakup time (TFBUT), cornea fluorescence score (CFS), and Schirmer test I value; and determined the optimal cutoff value of MBI to suspect DED using a receiver operating characteristic (ROC) analysis...
September 7, 2018: Scientific Reports
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